AR087189A1 - Polinucleotidos que codifican trehalosa-6-fosfato fosfatasa y sus metodos de uso - Google Patents
Polinucleotidos que codifican trehalosa-6-fosfato fosfatasa y sus metodos de usoInfo
- Publication number
- AR087189A1 AR087189A1 ARP120102555A ARP120102555A AR087189A1 AR 087189 A1 AR087189 A1 AR 087189A1 AR P120102555 A ARP120102555 A AR P120102555A AR P120102555 A ARP120102555 A AR P120102555A AR 087189 A1 AR087189 A1 AR 087189A1
- Authority
- AR
- Argentina
- Prior art keywords
- plant
- accordance
- increased
- t6pp
- sample
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01216—Trehalose 6-phosphate phosphorylase (2.4.1.216)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente se refiere generalmente al campo de la biología molecular y considera varios polinucleótidos, polipéptidos y métodos que se pueden emplear para aumentar el rendimiento en las plantas transgénicas. En forma específica, las plantas transgénicas pueden exhibir cualquiera de los rasgos que consisten en aumento del rendimiento, aumento de tolerancia al estrés abiótico, aumento de crecimiento celular y aumento de la eficiencia de uso de nutrientes.Reivindicación 1: Un polipéptido de T6PP aislado que comprende una modificación con alguna o una combinación de las siguientes modificaciones a) conversión de un residuo His a un residuo Asp en la posición de aminoácido 244 o una posición homóloga; b) conversión de Ala a Val en la posición 102 o a posición homóloga; y/o c) conversión de Ile a Phe en la posición 129 o a posición homóloga. Reivindicación 6: Una planta, célula de planta, parte de planta o muestra de planta que comprende el polipéptido de T6PP que se describe de acuerdo con cualquiera de las reivindicaciones 1 - 5. Reivindicación 8: Una muestra de planta que comprende el polipéptido de T6PP que se describe de acuerdo con cualquiera de las reivindicaciones 1 - 5. Reivindicación 9: Un polinucleótido que codifica el polipéptido de T6PP que se describe de acuerdo con cualquiera de las reivindicaciones 1 - 5. Reivindicación 22: Un método para aumentar el rendimiento en una planta, el método que comprende: a) introducir en una célula de planta el casete de expresión que se describe de acuerdo con cualquiera de las reivindicaciones 17 - 21; b) regenerar una planta de la célula de planta de a); y c) producir una planta con aumento del rendimiento. Reivindicación 28: Un método para aumentar la tolerancia de una planta al estrés abiótico, el método que comprende: a) introducir en una célula de planta el casete de expresión que se describe de acuerdo con cualquiera de las reivindicaciones 17 - 21; b) regenerar una planta de la célula de planta de a); y c) producir una planta con aumento de tolerancia al estrés abiótico. Reivindicación 34: Un kit para detectar ácidos nucleicos que son únicos para el polinucleótido de acuerdo con la reivindicación 9 ó 10, que comprende al menos una molécula de ácido nucleico de suficiente longitud de polinucleótidos contiguos para actuar como un cebador o sonda en un método de detección de ácido nucleico y que después de la amplificación de o hibridación a una secuencia de ácidos nucleicos blanco en una muestra seguido por la detección del amplicón o hibridación con la secuencia blanco, son diagnóstico para la presencia de las secuencias de ácidos nucleicos únicas para el polinucleótido de acuerdo con la reivindicación 9 ó 10 en la muestra.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161508605P | 2011-07-15 | 2011-07-15 | |
| US201161522549P | 2011-08-11 | 2011-08-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR087189A1 true AR087189A1 (es) | 2014-02-26 |
Family
ID=46584358
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP120102555A AR087189A1 (es) | 2011-07-15 | 2012-07-13 | Polinucleotidos que codifican trehalosa-6-fosfato fosfatasa y sus metodos de uso |
| ARP120102579A AR087206A1 (es) | 2011-07-15 | 2012-07-16 | Evento de maiz mzdt09y |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP120102579A AR087206A1 (es) | 2011-07-15 | 2012-07-16 | Evento de maiz mzdt09y |
Country Status (3)
| Country | Link |
|---|---|
| US (2) | US9121033B2 (es) |
| AR (2) | AR087189A1 (es) |
| WO (2) | WO2013012643A1 (es) |
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| WO2013012788A2 (en) * | 2011-07-15 | 2013-01-24 | Syngenta Participations Ag | Methods of increasing yield and stress tolerance in a plant |
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- 2012-07-13 AR ARP120102555A patent/AR087189A1/es unknown
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- 2012-07-16 US US13/549,794 patent/US9121033B2/en active Active
- 2012-07-16 US US13/549,693 patent/US20130019334A1/en not_active Abandoned
- 2012-07-16 WO PCT/US2012/046852 patent/WO2013012775A1/en active Application Filing
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| US9121033B2 (en) | 2015-09-01 |
| US20130019334A1 (en) | 2013-01-17 |
| US20130019342A1 (en) | 2013-01-17 |
| WO2013012643A1 (en) | 2013-01-24 |
| AR087206A1 (es) | 2014-02-26 |
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