AR085912A1 - Celulas mutantes para la secrecion de proteinas y degradacion de lignocelulosa - Google Patents

Celulas mutantes para la secrecion de proteinas y degradacion de lignocelulosa

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Publication number
AR085912A1
AR085912A1 ARP120100853A ARP120100853A AR085912A1 AR 085912 A1 AR085912 A1 AR 085912A1 AR P120100853 A ARP120100853 A AR P120100853A AR P120100853 A ARP120100853 A AR P120100853A AR 085912 A1 AR085912 A1 AR 085912A1
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AR
Argentina
Prior art keywords
cell
protein
cells
mutant cell
cellulosic biomass
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Application number
ARP120100853A
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English (en)
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Univ California
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Publication of AR085912A1 publication Critical patent/AR085912A1/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
    • C12Y101/99018—Cellobiose oxidase (1.1.99.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

También se proporcionan métodos para el uso de estas células. Específicamente se divulga la utilidad de las deleciones genéticas combinadas de b-glucosidasas y el gen represor de catabolitos creA/cre-1 para la secreción de proteínas en células de hongos y levaduras.Reivindicación 1: Un método para aumentar la secreción de una proteína desde una célula, método que comprende: (a) proporcionar una célula mutante, donde la célula mutante comprende mutaciones desactivantes en dos o más genes de b-glucosidasa; y (b) poner en contacto a dicha célula mutante con biomasa celulósica, donde dicha biomasa celulósica induce a la célula mutante a segregar dicha proteína. Reivindicación 56: Una célula mutante que comprende mutaciones desactivantes en dos o más genes de b-glucosidasa, donde la biomasa celulósica induce a la célula a segregar niveles más elevados de una proteína que los de una célula correspondiente que carece de dicha mutación en los dos genes o más de b-glucosidasa.
ARP120100853A 2011-03-15 2012-03-15 Celulas mutantes para la secrecion de proteinas y degradacion de lignocelulosa AR085912A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161453086P 2011-03-15 2011-03-15

Publications (1)

Publication Number Publication Date
AR085912A1 true AR085912A1 (es) 2013-11-06

Family

ID=45894690

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120100853A AR085912A1 (es) 2011-03-15 2012-03-15 Celulas mutantes para la secrecion de proteinas y degradacion de lignocelulosa

Country Status (9)

Country Link
US (2) US20140045243A1 (es)
EP (1) EP2686426A1 (es)
JP (1) JP2014508534A (es)
CN (1) CN103890168A (es)
AR (1) AR085912A1 (es)
AU (1) AU2012229063A1 (es)
BR (1) BR112013023756A2 (es)
CA (1) CA2829842A1 (es)
WO (1) WO2012125865A1 (es)

Families Citing this family (24)

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CN103305426B (zh) * 2012-03-16 2015-12-02 中国科学院天津工业生物技术研究所 产纤维素酶的突变菌株、高效表达目的蛋白的突变菌株及其构建方法与应用
CN104364358A (zh) * 2012-04-09 2015-02-18 Bp生物燃料英国有限公司 用于生产生物燃料和其他可再生材料的低多糖微生物
JP2014150745A (ja) * 2013-02-06 2014-08-25 Nagaoka Univ Of Technology トリコデルマ属に属する微生物の変異株および該変異株の使用
JP6103584B2 (ja) * 2013-03-04 2017-03-29 国立研究開発法人産業技術総合研究所 ｃｒｅ１遺伝子が機能しない糸状菌変異株
WO2015093467A1 (ja) * 2013-12-16 2015-06-25 味の素株式会社 セルラーゼ生産微生物
CN103911296B (zh) * 2014-04-21 2016-02-24 山东大学 一种提高纤维素酶和半纤维素酶酶活性的草酸青霉菌株
WO2016112238A1 (en) * 2015-01-09 2016-07-14 University Of Cincinnati Neurospora crassa strains with amplified expression of cellulases and production of biofuel therefrom
CN106119137B (zh) * 2015-05-06 2020-06-26 中国科学院天津工业生物技术研究所 一种改善丝状真菌蛋白分泌能力的方法
CN105671021B (zh) * 2016-03-01 2019-06-28 山东大学 甘露糖苷酶i的表达基因的应用
WO2018009806A1 (en) * 2016-07-07 2018-01-11 Novozymes A/S Methods of producing a fermentation product in trichoderma
CN106520726B (zh) * 2016-11-25 2017-09-19 山东大学 一种胞外醛糖酸内酯酶PoALAC及其应用
CN106755052B (zh) * 2016-12-15 2021-03-16 湖北工业大学 一种光调控启动子降低黑曲霉β-葡萄糖苷酶表达酶活的方法
CN106520822B (zh) * 2016-12-15 2019-10-11 湖北工业大学 一种磷酸盐诱导启动子降低黑曲霉β-葡萄糖苷酶表达酶活的方法
CN108795988B (zh) * 2017-05-04 2022-07-29 中国科学院分子植物科学卓越创新中心 木聚糖酶专性转录抑制因子及其在里氏木霉改造中的应用
CN107227261A (zh) * 2017-05-24 2017-10-03 陕西省微生物研究所 一种食用菌菌渣促腐复合菌剂及其制备方法
JP7384035B2 (ja) * 2017-09-25 2023-11-21 味の素株式会社 タンパク質の製造法および二糖の製造法
CN109694861B (zh) * 2017-10-24 2021-04-16 朱一民 人工纤维小体、利用其分解纤维素及生产酒精的方法
US11492603B2 (en) * 2018-03-26 2022-11-08 Toray Industries, Inc. Trichoderma reesei mutant and protein production method
CN109837232A (zh) * 2019-03-27 2019-06-04 黑龙江大学 一种粗糙脉胞菌外切葡聚糖酶突变菌株的构建方法和应用
AU2020295036A1 (en) * 2019-06-17 2022-01-06 Migal Galilee Research Institute Ltd. Stabilized mutants of quorum quenching lactonase and use thereof in treatment of pathogens
CN113106114B (zh) * 2020-01-13 2024-04-12 中国科学院分子植物科学卓越创新中心 调控里氏木霉蛋白表达效率的因子、调控方法及应用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN114409062A (zh) * 2021-12-31 2022-04-29 浙江巨能环境工程有限公司 一种用于强化废水处理生物膜的药剂与方法
CN115896049B (zh) * 2022-11-28 2024-02-02 中南林业科技大学 纤维二糖脱氢酶基因、载体、重组菌及它们的应用

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EP1225227B1 (en) * 1990-12-10 2009-02-18 Genencor International, Inc. Improved saccharification of cellulose by cloning and amplification of the beta-glucosidase gene of trichoderma reesei
US6982159B2 (en) * 2001-09-21 2006-01-03 Genencor International, Inc. Trichoderma β-glucosidase
JP4744879B2 (ja) * 2002-11-07 2011-08-10 ジェネンコー・インターナショナル・インク ＢＧＬ６β−グルコシダーゼ及びそれをエンコードする核酸
WO2004081201A1 (en) * 2003-03-12 2004-09-23 Vib Vzw Improved protein secretion in eukaryotic cells
WO2011123715A1 (en) 2010-03-31 2011-10-06 The United States Of America As Represented By The Secretary Of Agriculture Metabolically engineered yeasts for the production of ethanol and other products from xylose and cellobiose

Also Published As

Publication number Publication date
CA2829842A1 (en) 2012-09-20
US9822373B2 (en) 2017-11-21
US20150247152A1 (en) 2015-09-03
CN103890168A (zh) 2014-06-25
AU2012229063A1 (en) 2013-09-19
WO2012125865A1 (en) 2012-09-20
AU2012229063A2 (en) 2014-01-09
JP2014508534A (ja) 2014-04-10
EP2686426A1 (en) 2014-01-22
BR112013023756A2 (pt) 2016-09-20
US20140045243A1 (en) 2014-02-13

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