WO2012168743A3 - Electrogenic bacteria with heterologous mtr or omc genes - Google Patents

Electrogenic bacteria with heterologous mtr or omc genes Download PDF

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Publication number
WO2012168743A3
WO2012168743A3 PCT/GB2012/051319 GB2012051319W WO2012168743A3 WO 2012168743 A3 WO2012168743 A3 WO 2012168743A3 GB 2012051319 W GB2012051319 W GB 2012051319W WO 2012168743 A3 WO2012168743 A3 WO 2012168743A3
Authority
WO
WIPO (PCT)
Prior art keywords
electrogenic
heterologous
micro
omc
mtr
Prior art date
Application number
PCT/GB2012/051319
Other languages
French (fr)
Other versions
WO2012168743A2 (en
Inventor
Stephen R.P. JAFFÉ
Gregory J.S. FOWLER
Ana G. PEREIRA-MEDRANO
Phillip C. WRIGHT
Original Assignee
The University Of Sheffield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University Of Sheffield filed Critical The University Of Sheffield
Publication of WO2012168743A2 publication Critical patent/WO2012168743A2/en
Publication of WO2012168743A3 publication Critical patent/WO2012168743A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
    • H01M8/00—Fuel cells; Manufacture thereof
    • H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
    • Y02E60/30—Hydrogen technology
    • Y02E60/50—Fuel cells

Abstract

There is provided an electrogenic micro-organism host cell comprising at least one heterologous electrogenic pathway gene. The electrogenic micro-organism host cell may be especially characterised in that, in use in a microbial fuel cell, a greater power density is obtainable from the microbial fuel cell compared to the power density obtainable from a microbial fuel cell comprising an equivalent host cell not comprising the heterologous electrogenic pathway protein. There is also provided microbial fuel cells comprising such an electrogenic micro-organism host cell and methods of obtaining such electrogenic micro-organism host cells.
PCT/GB2012/051319 2011-06-10 2012-06-11 Bacteria WO2012168743A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1109756.5 2011-06-10
GBGB1109756.5A GB201109756D0 (en) 2011-06-10 2011-06-10 Bacteria

Publications (2)

Publication Number Publication Date
WO2012168743A2 WO2012168743A2 (en) 2012-12-13
WO2012168743A3 true WO2012168743A3 (en) 2013-03-21

Family

ID=44357530

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2012/051319 WO2012168743A2 (en) 2011-06-10 2012-06-11 Bacteria

Country Status (2)

Country Link
GB (1) GB201109756D0 (en)
WO (1) WO2012168743A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2706120B1 (en) * 2012-09-05 2016-04-20 Karlsruher Institut für Technologie Biosensor comprising a mtr-gene construct
CN104577143B (en) * 2015-01-13 2017-04-26 浙江大学 Method for preparing antibacterial air cathode
GB201501570D0 (en) * 2015-01-30 2015-03-18 Univ Bristol Apparatus
WO2016187195A1 (en) * 2015-05-21 2016-11-24 University Of Georgia Research Foundation, Inc. Engineered photosynthetic organisms, photosynthetic electrodes including the engineered photosynthetic organisms, photosynthetic bioelectrochemical cells and photosynthetic fuel cells
JP7039847B2 (en) * 2017-03-08 2022-03-23 東洋紡株式会社 Electronic mediator
CN110628689B (en) * 2019-10-18 2021-10-26 南京工业大学 Escherichia coli genetic engineering bacterium for producing succinic acid and construction method and application thereof
CN112921047A (en) * 2021-03-16 2021-06-08 天津大学 Shewanella electrogenesis strain for over-expressing cytochrome protein and construction method and application thereof
CN115976089A (en) * 2022-09-09 2023-04-18 天津大学 Method for improving electric energy output by engineering reinforced Shewanella vesicle secretion

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100279178A1 (en) * 2009-02-23 2010-11-04 Barkeloo Jason E Microbial fuel cell
US20100304189A1 (en) * 2009-05-27 2010-12-02 University Of Massachusetts Geobacteraceae strains and methods
WO2012037123A1 (en) * 2010-09-13 2012-03-22 Regents Of The University Of Minnesota Microbes, methods, and devices for redox-imbalanced metabolism
WO2012115278A1 (en) * 2011-02-24 2012-08-30 ソニー株式会社 Microbial fuel cell, fuel and microbes for said fuel cell, bioreactor and biosensor

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100279178A1 (en) * 2009-02-23 2010-11-04 Barkeloo Jason E Microbial fuel cell
US20100304189A1 (en) * 2009-05-27 2010-12-02 University Of Massachusetts Geobacteraceae strains and methods
WO2012037123A1 (en) * 2010-09-13 2012-03-22 Regents Of The University Of Minnesota Microbes, methods, and devices for redox-imbalanced metabolism
WO2012115278A1 (en) * 2011-02-24 2012-08-30 ソニー株式会社 Microbial fuel cell, fuel and microbes for said fuel cell, bioreactor and biosensor

Non-Patent Citations (6)

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Title
BUECKING CLEMENS ET AL: "Involvement and specificity of Shewanella oneidensis outer membrane cytochromes in the reduction of soluble and solid-phase terminal electron acceptors", FEMS MICROBIOLOGY LETTERS, vol. 306, no. 2, May 2010 (2010-05-01), pages 144 - 151, XP002683902, ISSN: 0378-1097 *
JENSEN: "Engineering of a synthetic electron conduit in living cells", 1 January 2010 (2010-01-01), http://www.pnas.org/content/early/2010/10/11/1009645107.full.pdf+html, XP055016322, Retrieved from the Internet <URL:http://www.pnas.org/content/early/2010/10/11/1009645107.full.pdf#page=1&view=FitH> [retrieved on 20120112], DOI: 10.1073/pnas.1009645107/-/DCSupplemental *
K. E. PITTS ET AL: "Characterization of the Shewanella oneidensis MR-1 Decaheme Cytochrome MtrA: EXPRESSION IN ESCHERICHIA COLI CONFERS THE ABILITY TO REDUCE SOLUBLE FE(III) CHELATES", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 30, 1 July 2003 (2003-07-01), pages 27758 - 27765, XP055016028, ISSN: 0021-9258, DOI: 10.1074/jbc.M302582200 *
MEITL L A ET AL: "Electrochemical interaction of Shewanella oneidensis MR-1 and its outer membrane cytochromes OmcA and MtrC with hematite electrodes", GEOCHIMICA ET COSMOCHIMICA ACTA, PERGAMON PRESS, NEW YORK, NY, US, vol. 73, no. 18, 15 September 2009 (2009-09-15), pages 5292 - 5307, XP026446762, ISSN: 0016-7037, [retrieved on 20090628] *
PHAM T H ET AL: "Bioanode performance in bioelectrochemical systems: recent improvements and prospects", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 27, no. 3, 1 March 2009 (2009-03-01), pages 168 - 178, XP025958234, ISSN: 0167-7799, [retrieved on 20090131], DOI: 10.1016/J.TIBTECH.2008.11.005 *
ROSENBAUM M ET AL: "Recombinant Engineering of Shewanella oneidensis MR-1 c-type Cytochromes in Escherichia coli", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, THE SOCIETY, WASHINGTON, DC, US, vol. 108, 1 June 2008 (2008-06-01), pages 621, XP002666976, ISSN: 1060-2011 *

Also Published As

Publication number Publication date
GB201109756D0 (en) 2011-07-27
WO2012168743A2 (en) 2012-12-13

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