AR083611A1 - Purificacion de anticuerpos mediante unidad simple de cromatografia de intercambio ionico - Google Patents

Purificacion de anticuerpos mediante unidad simple de cromatografia de intercambio ionico

Info

Publication number
AR083611A1
AR083611A1 ARP110104019A ARP110104019A AR083611A1 AR 083611 A1 AR083611 A1 AR 083611A1 AR P110104019 A ARP110104019 A AR P110104019A AR P110104019 A ARP110104019 A AR P110104019A AR 083611 A1 AR083611 A1 AR 083611A1
Authority
AR
Argentina
Prior art keywords
exchange chromatography
ion exchange
purification
antibodies
unit
Prior art date
Application number
ARP110104019A
Other languages
English (en)
Inventor
Marijke Yvonne Dorst
Diderik Reinder Kremer
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of AR083611A1 publication Critical patent/AR083611A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Método para la purificación de anticuerpos a partir de una mezcla de proteína producida en un biorreactor, que comprende por lo menos las etapas de purificación intermedia y de pulido, en donde la etapa intermedia y de pulido comprende cualquiera de las etapas de cromatografía en línea de cromatografía de intercambio aniónico (AEX) y cromatografía de intercambio catiónico (CEX) en modo de flujo pasante. Adicionalmente se provee una unidad operativa simple que comprende una parte de cromatografía de intercambio aniónico y una parte de cromatografía de intercambio catiónico en cualquier orden, que se conecta en serie, en donde la unidad comprende una entrada en el extremo anterior de la primera parte de cromatografía de intercambio jónico y una salida en el extremo posterior de la segunda parte de cromatografía de intercambio iónico y en donde la unidad también comprende una entrada entre la primera parte de cromatografía de intercambio iónico y la segunda parte de cromatografía de intercambio iónico.
ARP110104019A 2010-11-01 2011-10-28 Purificacion de anticuerpos mediante unidad simple de cromatografia de intercambio ionico AR083611A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP10189563 2010-11-01

Publications (1)

Publication Number Publication Date
AR083611A1 true AR083611A1 (es) 2013-03-06

Family

ID=43274560

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110104019A AR083611A1 (es) 2010-11-01 2011-10-28 Purificacion de anticuerpos mediante unidad simple de cromatografia de intercambio ionico

Country Status (13)

Country Link
US (1) US20130289247A1 (es)
EP (1) EP2635599A1 (es)
JP (1) JP2013540787A (es)
KR (1) KR20130131352A (es)
CN (1) CN103189390A (es)
AR (1) AR083611A1 (es)
AU (1) AU2011325341B2 (es)
CA (1) CA2814781A1 (es)
EA (1) EA201300524A1 (es)
IL (1) IL225720A0 (es)
MX (1) MX2013004720A (es)
TW (1) TW201229058A (es)
WO (1) WO2012059308A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL212911A0 (en) * 2011-05-16 2011-07-31 Omrix Biopharmaceuticals Ltd Immunoglobulin reduced in thrombogenic contaminants and preparation thereof
CN103665098B (zh) * 2012-09-20 2015-08-05 中国科学院大连化学物理研究所 双相柱膜蛋白质微反应器及其应用
EP2833139A1 (en) 2013-08-01 2015-02-04 SuppreMol GmbH In vitro method for determining the stability of compositions comprising soluble Fc gamma receptor(s)
GB201506868D0 (en) * 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method for protein purification
US20190366236A1 (en) * 2016-11-10 2019-12-05 Sandoz Ag Process for desalting of a protein solution
US20210122783A1 (en) * 2018-06-19 2021-04-29 Bristol-Myers Squibb Company Methods of purifying proteins using chromatography
WO2020183332A1 (en) * 2019-03-11 2020-09-17 Intas Pharmaceuticals Ltd. Purification of adalimumab using tandem chromatography
CN113518654B (zh) * 2019-03-21 2023-12-15 新加坡科技研究局 捕获和/或纯化靶标的方法
CN114040815B (zh) * 2019-09-05 2024-06-28 生物辐射实验室股份有限公司 阴离子交换-疏水混合模式色谱树脂
KR20220101168A (ko) 2019-11-22 2022-07-19 모르포시스 아게 이온 교환 크로마토그래피 동안 항체의 수율을 증가시키는 방법
US12050448B2 (en) 2021-01-29 2024-07-30 Tata Consultancy Services Limited Method and system to control a continuous bioprocessing

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4118912C1 (es) * 1991-06-08 1992-07-02 Biotest Pharma Gmbh, 6072 Dreieich, De
DK2270044T3 (en) * 1998-06-09 2015-01-26 Csl Behring Ag Liquid immunoglobulin G (IgG) product
US20040002081A1 (en) * 2001-12-18 2004-01-01 Boehringer Ingelheim International Gmbh And Bia Separations D.O.O. Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
JPWO2004087761A1 (ja) * 2003-03-31 2006-07-27 麒麟麦酒株式会社 ヒトモノクローナル抗体およびヒトポリクローナル抗体の精製
PT1678208E (pt) * 2003-10-27 2013-07-10 Wyeth Llc Remoção de agregados de elevado peso molecular utilizando cromatografia de hidroxiapatite
EP1963367A4 (en) * 2005-12-06 2009-05-13 Amgen Inc POLISHING STEPS IN MULTI-STAGE PROTEIN PURIFICATION TREATMENTS
CN101437839A (zh) * 2006-03-20 2009-05-20 米德列斯公司 蛋白质纯化
CN101541825B (zh) * 2006-08-28 2013-08-14 阿雷斯贸易股份有限公司 Fc融合蛋白的纯化方法
JP2010501622A (ja) * 2006-08-28 2010-01-21 アレス トレーディング ソシエテ アノニム Ｆｃ−融合タンパク質の精製法
WO2008051448A2 (en) * 2006-10-19 2008-05-02 Tolerx, Inc. Methods and compositions for efficient removal of protein a from binding molecule preparations
WO2008073620A2 (en) * 2006-11-02 2008-06-19 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines
US20080167450A1 (en) * 2007-01-05 2008-07-10 Hai Pan Methods of purifying proteins
MX2009007632A (es) * 2007-01-22 2009-07-24 Genentech Inc Precipitacion de polielectrolito y purificacion de proteinas.
JP5064558B2 (ja) * 2007-06-01 2012-10-31 エフ．ホフマン−ラ ロシュ アーゲー 免疫グロブリン精製
US9433922B2 (en) * 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
WO2009058769A1 (en) * 2007-10-30 2009-05-07 Schering Corporation Purification of antibodies containing hydrophobic variants
US20110105725A1 (en) * 2008-05-15 2011-05-05 Novo Nordisk A/S Antibody purification process
SG195574A1 (en) * 2008-10-20 2013-12-30 Abbott Lab Antibodies that bind to il-18 and methods of purifying the same
CA2738499A1 (en) * 2008-10-20 2010-04-29 Abbott Laboratories Viral inactivation during purification of antibodies
CN102224160A (zh) 2008-11-25 2011-10-19 通用电气健康护理生物科学股份公司 用于纯化治疗蛋白的水性两相萃取加强的沉淀方法
US9527010B2 (en) * 2009-09-25 2016-12-27 Ge Healthcare Bio-Sciences Corp. Separation system and method

Also Published As

Publication number Publication date
AU2011325341A1 (en) 2013-05-02
WO2012059308A1 (en) 2012-05-10
US20130289247A1 (en) 2013-10-31
IL225720A0 (en) 2013-06-27
MX2013004720A (es) 2013-05-28
AU2011325341B2 (en) 2015-12-17
CN103189390A (zh) 2013-07-03
EA201300524A1 (ru) 2013-08-30
KR20130131352A (ko) 2013-12-03
EP2635599A1 (en) 2013-09-11
CA2814781A1 (en) 2012-05-10
TW201229058A (en) 2012-07-16
JP2013540787A (ja) 2013-11-07

Similar Documents

Publication Publication Date Title
AR083611A1 (es) Purificacion de anticuerpos mediante unidad simple de cromatografia de intercambio ionico
BR112012020254A2 (pt) método para a purificação de anticorpos a partir de uma mistura de proteína e, unidade operacional única.
Kristensen et al. Ordered organelle degradation during starvation-induced autophagy
AU2012283775A8 (en) Inhibitors of Bruton's tyrosine kinase
NO20171811A1 (no) Fremgangsmåte for fremstilling av (1R,2R)-3-(3-dimetylamino-1-etyl-2-metyl-propyl)-fenol
BR112014012275A2 (pt) purificação de proteína com a utilização de tampão bis-tris
AU2016222301A1 (en) Isolation and purification of antibodies using Protein A affinity chromatography
AR078778A1 (es) Proteinas de union a antigenos de il-23
WO2008087184A3 (en) Process for the purification of fc-containing proteins
AU2010273974A8 (en) Simultaneous, integrated selection and evolution of antibody/protein performance and expression in production hosts
WO2016100261A3 (en) Method of treating cancer with cgamp or cgasmp
EA201301180A1 (ru) Основанная на bcma стратификация и терапия пациентов, страдающих множественной миеломой
WO2009021754A3 (en) Monospecific and multispecific antibodies and method of use
BR112012021576A2 (pt) composições estáveis contendo anticorpo.
GB0911870D0 (en) Optimised coding sequence and promoter
AR063840A1 (es) Anticuerpos humanos monoclonales para btla y metodos de uso
AR071242A1 (es) Anticuerpos anti-cd37
CO6731130A2 (es) Proceso para la preparación de 4-amino-5-fluoro-3-halo-6-picolinatos(sustituidos)
MX2012004711A (es) Aislamiento y purificacion de los anticuerpos anti-il-13 al usar cromatografia de afinidad con proteina a.
PE20150996A1 (es) Etanercept correctamente plegado de alta pureza y excelente rendimiento
TR201911082T4 (tr) K vitaminine bağımlı proteinlerin saflaştırılması için bir işlem.
RS53745B1 (en) 6-AMINO-2 - {[(1S) -1-METYLBUTIL] OKSI} -9- [5- (1-PIPERIDINIL) PENTIL] -7,9-DIHIDRO-8H-PURIN-8-ON-MALEAT
BR112014004239A2 (pt) inibidores de enzima ativadora de nedd8
WO2012054929A3 (en) Use of human serum albumin to decrease antigenicity of therapeutic proteins
CL2012002082A1 (es) Proceso para la preparacion de lacosamida, por hidroximetilación; compuestos intermediarios.

Legal Events

Date Code Title Description
FB Suspension of granting procedure