AR083085A1 - Construcciones geneticas utiles en la produccion de plantas transgenicas con contenido de nitrato reducido - Google Patents

Construcciones geneticas utiles en la produccion de plantas transgenicas con contenido de nitrato reducido

Info

Publication number
AR083085A1
AR083085A1 ARP110103463A ARP110103463A AR083085A1 AR 083085 A1 AR083085 A1 AR 083085A1 AR P110103463 A ARP110103463 A AR P110103463A AR P110103463 A ARP110103463 A AR P110103463A AR 083085 A1 AR083085 A1 AR 083085A1
Authority
AR
Argentina
Prior art keywords
promoter
transgenic plants
plants
leaves
nitrate
Prior art date
Application number
ARP110103463A
Other languages
English (en)
Inventor
Lay Pascaline Le
Tamburrino Juan Pablo Sanchez
Susan Davenport
Original Assignee
Cambridge Advanced Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1015875.6A external-priority patent/GB201015875D0/en
Priority claimed from GBGB1109073.5A external-priority patent/GB201109073D0/en
Application filed by Cambridge Advanced Tech filed Critical Cambridge Advanced Tech
Publication of AR083085A1 publication Critical patent/AR083085A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B13/00—Tobacco for pipes, for cigars, e.g. cigar inserts, or for cigarettes; Chewing tobacco; Snuff
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cultivation Of Plants (AREA)
  • Manufacture Of Tobacco Products (AREA)

Abstract

Construcciones genéticas que pueden usarse para elaborar plantas transgénicas. Las construcciones pueden reducir la concentración de nitrato en las plantas, particularmente en las hojas de las plantas, y pueden inducir un fenotipo similar a la senescencia. Células vegetales que han sido transformadas con estas construcciones. Plantas transgénicas. Métodos para producir plantas transgénicas y métodos para reducir el contenido de nitrato en las plantas. Hojas cosechadas, por ejemplo, hojas de tabaco, que han sido transformadas con las construcciones genéticas. Diversos artículos de tabaco, tales como artículos para fumar, que comprenden las hojas cosechadas mencionadas.Reivindicación 1: Una construcción genética caracterizada porque comprende un promotor unido operativamente a una secuencia codificante que codifica un polipéptido que presenta actividad de transporte de nitrato, con la condición de que el promotor no sea un promotor del virus en mosaico de la coliflor 35S. Reivindicación 3: Una construcción genética de acuerdo con la reivindicación 1 o la reivindicación 2, caracterizada porque el promotor es el promotor del virus del anillo grabado del clavel (CERV), el promotor de la plastocianina de la arveja, el promotor de la rubisco, el promotor de la nopalina sintetasa, el promotor de la proteína que se une a la clorofila a/b, el promotor de la glutenina de gran peso molecular, el promotor de la a,b-gliadina, el promotor de la hordeína, el promotor de la patatina o un promotor específico para la senescencia.
ARP110103463A 2010-09-22 2011-09-22 Construcciones geneticas utiles en la produccion de plantas transgenicas con contenido de nitrato reducido AR083085A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1015875.6A GB201015875D0 (en) 2010-09-22 2010-09-22 Transgenic plants
GBGB1109073.5A GB201109073D0 (en) 2011-05-31 2011-05-31 Transgenic plants

Publications (1)

Publication Number Publication Date
AR083085A1 true AR083085A1 (es) 2013-01-30

Family

ID=45873488

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110103463A AR083085A1 (es) 2010-09-22 2011-09-22 Construcciones geneticas utiles en la produccion de plantas transgenicas con contenido de nitrato reducido

Country Status (9)

Country Link
US (1) US9322029B2 (es)
EP (1) EP2619220A1 (es)
JP (1) JP5905011B2 (es)
CN (1) CN103403021A (es)
AP (1) AP2013006825A0 (es)
AR (1) AR083085A1 (es)
BR (1) BR112013006306A2 (es)
CA (1) CA2812380A1 (es)
WO (1) WO2012038717A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201204862D0 (en) * 2012-03-20 2012-05-02 Cambridge Advanced Tech Transgenic plants
GB201204869D0 (en) 2012-03-20 2012-05-02 Cambridge Advanced Tech Transgenic plants
GB201204871D0 (en) 2012-03-20 2012-05-02 Cambridge Advanced Tech Transgenic plants
US9670497B2 (en) * 2014-05-19 2017-06-06 Azargen Biotechnologies (Pty) Ltd. Synthetic promoter construct for transgene expression
US11666082B2 (en) * 2014-09-26 2023-06-06 Philip Morris Products S.A. Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway
GB201501941D0 (en) * 2015-02-05 2015-03-25 British American Tobacco Co Method
EP3414332B1 (en) * 2016-02-08 2022-06-29 Centre National De La Recherche Scientifique -Cnrs- Improvement of photosynthetic organisms through the modulation of guanosine tetraphosphate homeostatis
CN111212562B (zh) 2017-09-01 2023-06-27 奥驰亚客户服务有限公司 与烟草中提高的氮利用效率有关的方法和组合物
CN115786337A (zh) * 2022-08-26 2023-03-14 华中农业大学 一种来源于杨树的根特异启动子及其应用
CN117025626A (zh) * 2023-06-29 2023-11-10 中国烟草总公司郑州烟草研究院 烟草硝酸盐转运蛋白NtNPF7.4及其编码基因、基因编辑载体和应用

Family Cites Families (15)

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EP0320500B1 (en) 1983-01-13 2004-11-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Non-oncogenic ti plasmid vector system and recombinant DNA molecules for the introduction of expressible genes into plant cell genomes
US4643290A (en) 1985-12-04 1987-02-17 Sundstrand Corporation Bi-directional overtravel stop
ES2018274T5 (es) 1986-03-11 1996-12-16 Plant Genetic Systems Nv Celulas vegetales resistentes a los inhibidores de glutamina sintetasa, preparadas por ingenieria genetica.
EP0265556A1 (en) 1986-10-31 1988-05-04 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Stable binary agrobacterium vectors and their use
GB8626862D0 (en) 1986-11-11 1986-12-10 Jefferson R A Gene fusion
GB8825402D0 (en) 1988-10-31 1988-11-30 Cambridge Advanced Tech Sulfonamide resistance genes
AU2001289843A1 (en) 2001-08-28 2002-02-13 Bayer Cropscience Ag Polypeptides for identifying herbicidally active compounds
US20090183270A1 (en) 2002-10-02 2009-07-16 Adams Thomas R Transgenic plants with enhanced agronomic traits
CN1246463C (zh) * 2004-03-03 2006-03-22 清华大学 低氧诱导微生物表达载体及其构建方法与应用
EP1926818A2 (en) 2005-08-15 2008-06-04 E.I.Du pont de nemours and company Nitrate transport components
CN101395275A (zh) * 2005-08-15 2009-03-25 纳幕尔杜邦公司 硝酸盐转运组分
US8853492B2 (en) * 2005-11-07 2014-10-07 Cropdesign N.V. Plants having improved growth characteristics and a method for making the same
GB0715916D0 (en) * 2007-08-15 2007-09-26 Cambridge Advanced Tech Production of modified plants
US20100206317A1 (en) * 2007-09-28 2010-08-19 Vector Tobacco, Inc. Reduced risk tobacco products and use thereof
MX2011004216A (es) * 2008-11-04 2011-05-25 Pioneer Hi Bred Int Gen at1g67330 novedoso en la eficiencia de captacion de nitrato alterada.

Also Published As

Publication number Publication date
US20130206155A1 (en) 2013-08-15
CN103403021A (zh) 2013-11-20
US9322029B2 (en) 2016-04-26
AP2013006825A0 (en) 2013-04-30
BR112013006306A2 (pt) 2016-06-07
JP2013543377A (ja) 2013-12-05
EP2619220A1 (en) 2013-07-31
WO2012038717A1 (en) 2012-03-29
CA2812380A1 (en) 2012-03-29
JP5905011B2 (ja) 2016-04-20

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