AR070608A2 - Plantas morfologicamente normales, completas y fertiles que comprenden un gen de epsps modificado - Google Patents
Plantas morfologicamente normales, completas y fertiles que comprenden un gen de epsps modificadoInfo
- Publication number
- AR070608A2 AR070608A2 ARP090100472A ARP090100472A AR070608A2 AR 070608 A2 AR070608 A2 AR 070608A2 AR P090100472 A ARP090100472 A AR P090100472A AR P090100472 A ARP090100472 A AR P090100472A AR 070608 A2 AR070608 A2 AR 070608A2
- Authority
- AR
- Argentina
- Prior art keywords
- rice
- epsps
- transcription
- enhancer
- transformed
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
- C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01019—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Un material de planta que ha sido transformado con un polinucleotido que comprende los siguientes componentes en la direccion 5' a 3' de la transcripcion: (i) al menos un potenciador de transcripcion donde la region potenciadora que está corriente arriba del inicio de la transcripcion de la secuencia a partir del cual se obtiene el potenciador y cuyo potenciador per se no funciona como un promotor ya sea en la secuencia en la cual está comprendido en forma endogena o cuando está presente heterologamente como parte de una construccion, (ii) el promotor del gen de la EPSPS de arroz, (iii) la secuencia genomica de arroz que codifica el péptido de tránsito a cloroplasto de la EPSPS de arroz, (iv) la secuencia genomica que codifica la EPSPS de arroz, (v) un terminador de transcripcion, donde la secuencia que codifica la EPSPS de arroz está modificada en que una primera posicion está mutada de tal modo que el residuo en esa posicion es Ile en lugar de Thr y una segunda posicion está mutada de tal modo que el residuo en esa posicion es Ser en lugar de Pro, donde las mutaciones se introducen en las secuencias de EPSPS que comprenden la siguiente region conservada GNAGTAMRPLTAAV en la enzima salvaje de modo que la secuencia modificada se lee GNAGIAMRSLTAAV; o que ha sido transformado con un vector que comprende el polinucleotido descripto anteriormente; o que ha sido, o es, además transformado con un polinucleotido que comprende las regiones que codifican proteínas capaces de conferir a un material vegetal que contiene uno de siguientes rasgos agronomicamente deseables: resistencia a insectos, a hongos, a virus, a bacteria, a nematodos, a stress, a desecacion, y a herbicidas; y plantas morfologicamente normales, completas y fértiles.
Applications Claiming Priority (11)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9930213.5A GB9930213D0 (en) | 1999-04-29 | 1999-04-29 | Improvements in or relating to organic compounds |
GBGB9917834.5A GB9917834D0 (en) | 1999-04-29 | 1999-04-29 | Improvements in or relating to organic compounds |
GBGB9909968.1A GB9909968D0 (en) | 1999-04-29 | 1999-04-29 | Improvements in or relating to organic compounds |
GBGB9917840.2A GB9917840D0 (en) | 1999-07-29 | 1999-07-29 | Improvements in or relating to organic compounds |
GBGB9917847.7A GB9917847D0 (en) | 1999-07-29 | 1999-07-29 | Improvements in or relating to organic compounds |
GBGB9917839.4A GB9917839D0 (en) | 1999-07-29 | 1999-07-29 | Improvements in or relating to organic compounds |
GBGB9917846.9A GB9917846D0 (en) | 1999-07-29 | 1999-07-29 | Improvements in or relating to organic compounds |
GBGB9930200.2A GB9930200D0 (en) | 1999-12-21 | 1999-12-21 | Improvements in or relating to organic compounds |
GBGB9930209.3A GB9930209D0 (en) | 1999-12-21 | 1999-12-21 | Improvements in or relating to organic compounds |
GBGB9930204.4A GB9930204D0 (en) | 1999-12-21 | 1999-12-21 | Improvements in or relating to organic compounds |
GBGB9930207.7A GB9930207D0 (en) | 1999-12-21 | 1999-12-21 | Improvements in or relating to organic compounds |
Publications (1)
Publication Number | Publication Date |
---|---|
AR070608A2 true AR070608A2 (es) | 2010-04-21 |
Family
ID=27581692
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP090100472A AR070608A2 (es) | 1999-04-29 | 2009-02-11 | Plantas morfologicamente normales, completas y fertiles que comprenden un gen de epsps modificado |
Country Status (16)
Country | Link |
---|---|
US (1) | US6867293B2 (es) |
EP (1) | EP1173582B1 (es) |
JP (1) | JP2003528571A (es) |
CN (1) | CN1360634A (es) |
AR (1) | AR070608A2 (es) |
AT (1) | ATE330015T1 (es) |
AU (1) | AU4134000A (es) |
CA (1) | CA2365592C (es) |
CZ (1) | CZ20013860A3 (es) |
DK (1) | DK1173582T3 (es) |
ES (1) | ES2262514T3 (es) |
HU (1) | HUP0300187A3 (es) |
IL (1) | IL146062A0 (es) |
MX (1) | MXPA01010921A (es) |
PL (1) | PL357523A1 (es) |
WO (1) | WO2000066748A1 (es) |
Families Citing this family (132)
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IL146062A0 (en) | 1999-04-29 | 2002-07-25 | Marlow Foods Ltd | Herbicide resistant plants |
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US5310667A (en) * | 1989-07-17 | 1994-05-10 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases |
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CN1262661C (zh) * | 1997-08-07 | 2006-07-05 | 奥本大学 | 通用的叶绿体整合和表达载体、其转化植物及产物 |
IL146062A0 (en) | 1999-04-29 | 2002-07-25 | Marlow Foods Ltd | Herbicide resistant plants |
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- 2000-04-20 DK DK00920929T patent/DK1173582T3/da active
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- 2000-04-20 EP EP00920929A patent/EP1173582B1/en not_active Expired - Lifetime
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PL357523A1 (en) | 2004-07-26 |
CZ20013860A3 (cs) | 2002-04-17 |
CN1360634A (zh) | 2002-07-24 |
JP2003528571A (ja) | 2003-09-30 |
HUP0300187A3 (en) | 2004-11-29 |
IL146062A0 (en) | 2002-07-25 |
AU4134000A (en) | 2000-11-17 |
EP1173582B1 (en) | 2006-06-14 |
CA2365592C (en) | 2011-11-15 |
HUP0300187A2 (en) | 2003-05-28 |
ES2262514T3 (es) | 2006-12-01 |
WO2000066748A1 (en) | 2000-11-09 |
MXPA01010921A (es) | 2003-06-24 |
EP1173582A1 (en) | 2002-01-23 |
US20030077801A1 (en) | 2003-04-24 |
ATE330015T1 (de) | 2006-07-15 |
CA2365592A1 (en) | 2000-11-09 |
US6867293B2 (en) | 2005-03-15 |
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