AR067135A1 - Metodos para aislar arn de fragmento largo de muestras fijas - Google Patents

Metodos para aislar arn de fragmento largo de muestras fijas

Info

Publication number
AR067135A1
AR067135A1 ARP080102695A ARP080102695A AR067135A1 AR 067135 A1 AR067135 A1 AR 067135A1 AR P080102695 A ARP080102695 A AR P080102695A AR P080102695 A ARP080102695 A AR P080102695A AR 067135 A1 AR067135 A1 AR 067135A1
Authority
AR
Argentina
Prior art keywords
methods
long fragment
fixed samples
fragment rna
isolate long
Prior art date
Application number
ARP080102695A
Other languages
English (en)
Inventor
Kathleen Danenberg
Original Assignee
Response Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Response Genetics Inc filed Critical Response Genetics Inc
Publication of AR067135A1 publication Critical patent/AR067135A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La presente se relaciona con métodos para la extraccion de ARN desde especimenes de tejido fijado en formalina incrustado en parafina para su uso en aplicaciones biologicas, que incluyen ensayos basados en la hibridacion de oligonucleotidos.
ARP080102695A 2007-06-22 2008-06-23 Metodos para aislar arn de fragmento largo de muestras fijas AR067135A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US94578507P 2007-06-22 2007-06-22

Publications (1)

Publication Number Publication Date
AR067135A1 true AR067135A1 (es) 2009-09-30

Family

ID=39951594

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP080102695A AR067135A1 (es) 2007-06-22 2008-06-23 Metodos para aislar arn de fragmento largo de muestras fijas

Country Status (12)

Country Link
US (1) US20090092979A1 (es)
EP (1) EP2173874A2 (es)
JP (1) JP2010530761A (es)
KR (1) KR20100012056A (es)
CN (1) CN101784663A (es)
AR (1) AR067135A1 (es)
AU (1) AU2008268467A1 (es)
CA (1) CA2691209A1 (es)
IL (1) IL202889A0 (es)
NZ (1) NZ582219A (es)
TW (1) TW200911988A (es)
WO (1) WO2009002937A2 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011203450B2 (en) * 2010-01-04 2016-01-07 Qiagen Gaithersburg, Inc. Methods, compositions, and kits for recovery of nucleic acids or proteins from fixed tissue samples
GB201002717D0 (en) * 2010-02-18 2010-04-07 Rolls Royce Plc An apparatus and a method of determining the quality of a friction weld
WO2011104027A1 (en) * 2010-02-26 2011-09-01 Qiagen Gmbh Process for parallel isolation and/or purification of rna and dna
CN102822341A (zh) * 2010-04-08 2012-12-12 恰根有限公司 选择性分离和纯化核酸的方法
US20130095473A1 (en) * 2010-06-14 2013-04-18 Qiagen Gmbh Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples
TWI425977B (zh) 2010-11-24 2014-02-11 Ind Tech Res Inst 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組
EP2744916A4 (en) 2011-07-13 2015-06-17 Primeradx Inc MULTIMODAL METHODS FOR SIMULTANEOUS DETECTION AND QUANTIFICATION OF MULTIPLE NUCLEIC ACIDS IN A SAMPLE
US9416356B2 (en) 2013-03-15 2016-08-16 Abbott Molecular Inc. Compositions and methods for nucleic acid extraction
CN103725672B (zh) * 2013-10-29 2016-04-27 厦门艾德生物医药科技股份有限公司 一种从福尔马林固定石蜡包埋组织中共分离dna和rna的试剂盒及方法
US9771572B2 (en) 2014-02-28 2017-09-26 Gen-Probe Incorporated Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde
US9771571B2 (en) 2014-02-28 2017-09-26 Gen-Probe Incorporated Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde
USRE48665E1 (en) * 2014-02-28 2021-08-03 Gen-Probe Incorporated Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde
JP2023521579A (ja) * 2020-03-31 2023-05-25 キアゲン ゲーエムベーハー 固定された生体試料からの核酸精製

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5705336A (en) 1995-03-07 1998-01-06 The United States Of America As Represented By The Department Of Health And Human Services Assay for sensitivity of tumors to DNA-platinating chemotherapy
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US6518416B1 (en) 2000-12-01 2003-02-11 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
US7005278B2 (en) 2001-03-02 2006-02-28 Danenberg Kathleen D Method of determining dihydropyrimidine dehydrogenase gene expression
US6582919B2 (en) 2001-06-11 2003-06-24 Response Genetics, Inc. Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates
US7049059B2 (en) 2000-12-01 2006-05-23 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression
US20050059054A1 (en) 2003-07-25 2005-03-17 Richard Conrad Methods and compositions for preparing RNA from a fixed sample
US7544471B2 (en) 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen

Also Published As

Publication number Publication date
CA2691209A1 (en) 2008-12-31
US20090092979A1 (en) 2009-04-09
CN101784663A (zh) 2010-07-21
JP2010530761A (ja) 2010-09-16
KR20100012056A (ko) 2010-02-04
EP2173874A2 (en) 2010-04-14
TW200911988A (en) 2009-03-16
NZ582219A (en) 2012-03-30
WO2009002937A3 (en) 2009-02-12
IL202889A0 (en) 2011-08-01
WO2009002937A2 (en) 2008-12-31
AU2008268467A1 (en) 2008-12-31

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Legal Events

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FA Abandonment or withdrawal