AR051837A1 - Produccion de proteinas de fusion recombinante dentro de ensamblajes similares a cuerpos proteicos recombinantes (escpr) - Google Patents

Produccion de proteinas de fusion recombinante dentro de ensamblajes similares a cuerpos proteicos recombinantes (escpr)

Info

Publication number
AR051837A1
AR051837A1 ARP050104995A ARP050104995A AR051837A1 AR 051837 A1 AR051837 A1 AR 051837A1 AR P050104995 A ARP050104995 A AR P050104995A AR P050104995 A ARP050104995 A AR P050104995A AR 051837 A1 AR051837 A1 AR 051837A1
Authority
AR
Argentina
Prior art keywords
recombinant
protein
fusion protein
escpr
sequence
Prior art date
Application number
ARP050104995A
Other languages
English (en)
Inventor
Mugica Maria Dolores Ludevid
Virgili Miriam Bastida
Royo Blanca Llompart
Luna Pablo Marzabal
Quetglas Margarita Torrent
Original Assignee
Era Plantech S L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Era Plantech S L filed Critical Era Plantech S L
Publication of AR051837A1 publication Critical patent/AR051837A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/425—Zeins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se describe un procedimiento para formar una proteína de fusion que se expresa como un ensamblaje recombinante similar a cuerpos proteicos en las células eucariotas hospedadores y organismos como sistemas hospedadores, excepto en plantas superiores. Más específicamente, los péptidos y proteínas se funden en secuencias de proteínas que median en la induccion de la formacion de ensamblajes similares a cuerpos proteicos recombinantes (ESCPR), y se expresan y acumulan con estabilidad en estas células hospedadoras después de la transformacion con un vector adecuado. También se desvelan procedimientos para preparar la proteína de fusion. Reivindicacion 1: Una célula hospedadora eucariota de plantas no superiores que contiene proteína de fusion recombinante dentro de ensamblajes similares a cuerpos proteicos recombinantes (ESCPR), conteniendo dicha proteína de fusion dos secuencias enlazadas entre sí en las que una secuencia es una secuencia inductora de cuerpos proteicos heterologa con respecto a la célula hospedadora y la otra es la secuencia de un producto de interés. Reivindicacion 19: Una molécula de ácido nucleico recombinante que comprende un vector que contiene una o más secuencias reguladoras enlazadas operativamente a un segmento de ácido nucleico exogeno que define un gen que codifica una proteína de fusion que comprende i) una secuencia inductora de cuerpos proteicos que está enlazada a ii) un producto de interés polipéptido.
ARP050104995A 2004-11-29 2005-11-29 Produccion de proteinas de fusion recombinante dentro de ensamblajes similares a cuerpos proteicos recombinantes (escpr) AR051837A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0426160.8A GB0426160D0 (en) 2004-11-29 2004-11-29 Production of proteins

Publications (1)

Publication Number Publication Date
AR051837A1 true AR051837A1 (es) 2007-02-14

Family

ID=33561506

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP050104995A AR051837A1 (es) 2004-11-29 2005-11-29 Produccion de proteinas de fusion recombinante dentro de ensamblajes similares a cuerpos proteicos recombinantes (escpr)

Country Status (12)

Country Link
US (1) US8822181B2 (es)
EP (1) EP1819725B1 (es)
JP (1) JP2008521391A (es)
KR (1) KR101430081B1 (es)
CN (1) CN101098886B (es)
AR (1) AR051837A1 (es)
AU (1) AU2005308921B2 (es)
BR (1) BRPI0517121A (es)
CA (1) CA2589164C (es)
GB (1) GB0426160D0 (es)
RU (1) RU2429243C2 (es)
WO (1) WO2006056483A1 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
GB0426161D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Protein isolation and purification
US8163880B2 (en) 2006-02-23 2012-04-24 Era Biotech S.A. Production of biologically active proteins
JP5667062B2 (ja) 2008-10-10 2015-02-12 エラ、ビオテック、ソシエダッド、アノニマＥｒａ Ｂｉｏｔｅｃｈ， Ｓ．Ａ． 免疫原特異的アジュバントとしての組換えタンパク粒
EP2418284A1 (en) 2010-08-13 2012-02-15 ERA Biotech, S.A. Protein body-inducing polypeptide sequences
EP2468872A1 (en) * 2010-12-23 2012-06-27 Philip Morris Products S.A. Method for producing mullerian inhibitor substance in plants
EP2665818B1 (en) 2011-01-17 2017-03-22 Philip Morris Products S.a.s. Protein expression in plants
CN103502455A (zh) 2011-01-17 2014-01-08 菲利普莫里斯生产公司 用于在植物中核酸表达的载体
FI20115704L (fi) 2011-07-01 2013-01-02 Teknologian Tutkimuskeskus Vtt Oy Vierasproteiinien tuoton parantaminen
GB201514648D0 (en) * 2015-08-18 2015-09-30 Univ Cape Town Synthetic BTV VP2 fusion protein
GB201514652D0 (en) * 2015-08-18 2015-09-30 Univ Cape Town Synthetic BTV VP2 multiepitope peptide vaccine
TWI794170B (zh) * 2016-05-06 2023-03-01 英商葛蘭素史密斯克藍智慧財產發展有限公司 製造重組蛋白質之方法
CN112048518B (zh) * 2020-08-22 2022-06-24 江西农业大学 一种玉米醇溶蛋白降解酶的制备方法及其应用
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
CA3191387A1 (en) 2020-09-30 2022-04-07 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4215040A (en) 1978-12-22 1980-07-29 The Procter & Gamble Company Density separation process
US5650554A (en) * 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
ES2276475T5 (es) 1997-09-30 2014-07-11 The Regents Of The University Of California Producción de proteínas en semillas de plantas
AU2002256310A1 (en) 2001-04-18 2002-11-05 New Mexico State University Technology Transfer Corporation Co-expression of zein proteins
EP1532261B1 (en) 2002-05-24 2010-02-10 Medtronic, Inc. Methods and dna constructs for high yield production of polypeptides
DE60330564D1 (de) * 2002-05-24 2010-01-28 Restoragen Inc Verfahren und konstrukte zur expression von clostripain mit hoher ausbeute
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
GB0426161D0 (en) 2004-11-29 2004-12-29 Era Plantech S L Protein isolation and purification
US8163880B2 (en) 2006-02-23 2012-04-24 Era Biotech S.A. Production of biologically active proteins
JP5667062B2 (ja) 2008-10-10 2015-02-12 エラ、ビオテック、ソシエダッド、アノニマＥｒａ Ｂｉｏｔｅｃｈ， Ｓ．Ａ． 免疫原特異的アジュバントとしての組換えタンパク粒
EP2418284A1 (en) 2010-08-13 2012-02-15 ERA Biotech, S.A. Protein body-inducing polypeptide sequences

Also Published As

Publication number Publication date
EP1819725A1 (en) 2007-08-22
US20060121573A1 (en) 2006-06-08
AU2005308921A1 (en) 2006-06-01
AU2005308921B2 (en) 2011-11-24
JP2008521391A (ja) 2008-06-26
CA2589164A1 (en) 2006-06-01
KR20070091156A (ko) 2007-09-07
WO2006056483A1 (en) 2006-06-01
CN101098886A (zh) 2008-01-02
KR101430081B1 (ko) 2014-08-13
RU2429243C2 (ru) 2011-09-20
BRPI0517121A (pt) 2008-09-30
GB0426160D0 (en) 2004-12-29
EP1819725B1 (en) 2013-03-27
US8822181B2 (en) 2014-09-02
CA2589164C (en) 2014-10-28
CN101098886B (zh) 2012-09-26
RU2007124369A (ru) 2009-01-10

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