AR050201A1 - Acidos nucleicos que codifican agentes antihelminticos y plantas obtenidas a partir de estos. - Google Patents

Acidos nucleicos que codifican agentes antihelminticos y plantas obtenidas a partir de estos.

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Publication number
AR050201A1
AR050201A1 ARP050103261A ARP050103261A AR050201A1 AR 050201 A1 AR050201 A1 AR 050201A1 AR P050103261 A ARP050103261 A AR P050103261A AR P050103261 A ARP050103261 A AR P050103261A AR 050201 A1 AR050201 A1 AR 050201A1
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AR
Argentina
Prior art keywords
polypeptide
plant
plants
fatty acid
transgenic plants
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Application number
ARP050103261A
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English (en)
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Divergence Inc
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Application filed by Divergence Inc filed Critical Divergence Inc
Publication of AR050201A1 publication Critical patent/AR050201A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00Production of fats or fatty oils from raw materials
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223Vegetative tissue-specific promoters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se provee construcciones de ADN, plantas transgénicas que contienen tales construcciones, y métodos para obtener las plantas. Las construcciones de ADN codifican un polipéptido que cuandio se expresa da lugar a la produccion de ácidos grasos que tienen actividad antihelmíntica. Las plantas transgénicas que expresan tal polipéptido pueden exhibir mayor resistencia a los nematodos parasitarios de las plantas, en particular cuando se expresa en tejidos vegetativos. Las plantas transgénicas que expresan tal polipéptido también pueden ser utiles para usos industriales no plaguicidas. Reivindicacion 1: Una planta transgénica que contiene por lo menos una cosntruccion de ADN, comprendiendo dicha construccion: a) un ácido nucleico que codifica un polipéptido efectivo para catalizar la conversion de un sustrato en un ácido graso producto monoinsaturado de C16, C18 o C20 seleccionado del grupo formado por: (i) X-O-(O)C-CH2-CH2-CH2-CH2-CH2-CH2-CH2-C=C-CH2-C(R1)-C(R2)-CH2-R3 en el cual X es H, CoA, glicerol, un monoglicérido, un diglicérido, ACP, metilo, Na+, fosfatidilcolina, o fosfatidiletanolamina, donde ambos R1 y R2 son hidroxilo, uno de R1 y R2 es hidroxilo y el otro es H, o uno de R1 y R2 es ceto y el otro es H, y donde R3 es alquilo C2, C4, o C6; y ii) X-O-C(O)-CH2-CH2-CH2-CH2-CH2-CH2-CH2-C=C-CH2-C-(O)-C-CH2-R3 en el cual X es H, CoA, glicerol, un monoglicérido, un diglicérido, ACP, metilo, Na+, fosfatidilcolina, o fosfatidiletanolamina, y donde R3 es alquilo C2, C4, o C6; y (b) un elemento regulador ligado de manera funcional a dicho ácido nucleico que codifica dicho polipéptido, donde dicho elemento regulador confiere expresion en un tejido vegetativo de dicha planta. Reivindicacion 115: La planta de la reivindicacion 114, en la cual dicho ácido graso hidroxilado es ácido ricinileico. Reivindicacion 117: La planta de la reivindicaion 116, en la cual dicho ácido graso epoxidado es ácido vernolico.
ARP050103261A 2004-08-04 2005-08-04 Acidos nucleicos que codifican agentes antihelminticos y plantas obtenidas a partir de estos. AR050201A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10/912,534 US7368629B2 (en) 2004-02-04 2004-08-04 Nucleic acids encoding anthelmintic agents and plants made therefrom

Publications (1)

Publication Number Publication Date
AR050201A1 true AR050201A1 (es) 2006-10-04

Family

ID=35839881

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP050103261A AR050201A1 (es) 2004-08-04 2005-08-04 Acidos nucleicos que codifican agentes antihelminticos y plantas obtenidas a partir de estos.

Country Status (12)

Country Link
US (4) US7368629B2 (es)
EP (2) EP2182062A3 (es)
JP (1) JP2008508877A (es)
CN (1) CN101076588A (es)
AR (1) AR050201A1 (es)
AU (1) AU2005271476A1 (es)
BR (1) BRPI0513080A (es)
CA (1) CA2575748A1 (es)
IL (6) IL181103A0 (es)
MX (1) MX2007001346A (es)
WO (1) WO2006017577A2 (es)
ZA (1) ZA200700960B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
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US7368629B2 (en) 2004-02-04 2008-05-06 Divergence, Inc. Nucleic acids encoding anthelmintic agents and plants made therefrom
AU2005323136A1 (en) * 2004-12-20 2006-07-13 Basf Plant Science Gmbh Nucleic acid molecules encoding fatty acid desaturase genes from plants and methods of use
AU2007249224B2 (en) 2006-05-12 2013-03-21 Monsanto Technology Llc Methods and compositions for obtaining marker-free transgenic plants
US8431772B1 (en) 2008-11-19 2013-04-30 University Of Kentucky Research Foundation Diacylglycerol acyltransferase sequences and related methods
WO2012071439A1 (en) * 2010-11-22 2012-05-31 The Regents Of The University Of California Host cells and methods for producing diacid compounds
AU2013255080A1 (en) * 2012-04-30 2014-12-18 Commonwealth Scientific And Industrial Research Organisation Fatty acid modification and tag assembly genes
AR091774A1 (es) 2012-07-16 2015-02-25 Dow Agrosciences Llc Proceso para el diseño de las secuencias de adn repetidas, largas, divergentes de codones optimizados
JP7362487B2 (ja) * 2018-01-12 2023-10-17 亨将 吉武 有害生物忌避方法、及び次亜塩素酸水溶液の製造方法並びに製造装置

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EP2182062A3 (en) 2010-10-06
WO2006017577A3 (en) 2007-07-26
US20080250530A1 (en) 2008-10-09
EP1773110A2 (en) 2007-04-18
CN101076588A (zh) 2007-11-21
CA2575748A1 (en) 2006-02-16
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IL208559A0 (en) 2010-12-30
US7919680B2 (en) 2011-04-05
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US7667095B2 (en) 2010-02-23
IL208561A0 (en) 2010-12-30
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