AR050201A1 - NUCLEIC ACIDS THAT CODIFY ANTIHELMINTIC AGENTS AND PLANTS OBTAINED FROM THESE. - Google Patents

NUCLEIC ACIDS THAT CODIFY ANTIHELMINTIC AGENTS AND PLANTS OBTAINED FROM THESE.

Info

Publication number
AR050201A1
AR050201A1 ARP050103261A ARP050103261A AR050201A1 AR 050201 A1 AR050201 A1 AR 050201A1 AR P050103261 A ARP050103261 A AR P050103261A AR P050103261 A ARP050103261 A AR P050103261A AR 050201 A1 AR050201 A1 AR 050201A1
Authority
AR
Argentina
Prior art keywords
polypeptide
plant
plants
fatty acid
transgenic plants
Prior art date
Application number
ARP050103261A
Other languages
Spanish (es)
Original Assignee
Divergence Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Divergence Inc filed Critical Divergence Inc
Publication of AR050201A1 publication Critical patent/AR050201A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se provee construcciones de ADN, plantas transgénicas que contienen tales construcciones, y métodos para obtener las plantas. Las construcciones de ADN codifican un polipéptido que cuandio se expresa da lugar a la produccion de ácidos grasos que tienen actividad antihelmíntica. Las plantas transgénicas que expresan tal polipéptido pueden exhibir mayor resistencia a los nematodos parasitarios de las plantas, en particular cuando se expresa en tejidos vegetativos. Las plantas transgénicas que expresan tal polipéptido también pueden ser utiles para usos industriales no plaguicidas. Reivindicacion 1: Una planta transgénica que contiene por lo menos una cosntruccion de ADN, comprendiendo dicha construccion: a) un ácido nucleico que codifica un polipéptido efectivo para catalizar la conversion de un sustrato en un ácido graso producto monoinsaturado de C16, C18 o C20 seleccionado del grupo formado por: (i) X-O-(O)C-CH2-CH2-CH2-CH2-CH2-CH2-CH2-C=C-CH2-C(R1)-C(R2)-CH2-R3 en el cual X es H, CoA, glicerol, un monoglicérido, un diglicérido, ACP, metilo, Na+, fosfatidilcolina, o fosfatidiletanolamina, donde ambos R1 y R2 son hidroxilo, uno de R1 y R2 es hidroxilo y el otro es H, o uno de R1 y R2 es ceto y el otro es H, y donde R3 es alquilo C2, C4, o C6; y ii) X-O-C(O)-CH2-CH2-CH2-CH2-CH2-CH2-CH2-C=C-CH2-C-(O)-C-CH2-R3 en el cual X es H, CoA, glicerol, un monoglicérido, un diglicérido, ACP, metilo, Na+, fosfatidilcolina, o fosfatidiletanolamina, y donde R3 es alquilo C2, C4, o C6; y (b) un elemento regulador ligado de manera funcional a dicho ácido nucleico que codifica dicho polipéptido, donde dicho elemento regulador confiere expresion en un tejido vegetativo de dicha planta. Reivindicacion 115: La planta de la reivindicacion 114, en la cual dicho ácido graso hidroxilado es ácido ricinileico. Reivindicacion 117: La planta de la reivindicaion 116, en la cual dicho ácido graso epoxidado es ácido vernolico.DNA constructs, transgenic plants containing such constructs, and methods for obtaining the plants are provided. The DNA constructs encode a polypeptide which when expressed results in the production of fatty acids that have anthelmintic activity. Transgenic plants that express such a polypeptide may exhibit greater resistance to parasitic nematodes of plants, particularly when expressed in vegetative tissues. Transgenic plants that express such a polypeptide can also be useful for non-pesticide industrial uses. Claim 1: A transgenic plant containing at least one DNA construction, said construction comprising: a) a nucleic acid encoding an effective polypeptide to catalyze the conversion of a substrate into a selected C16, C18 or C20 monounsaturated fatty acid. from the group consisting of: (i) XO- (O) C-CH2-CH2-CH2-CH2-CH2-CH2-CH2-C = C-CH2-C (R1) -C (R2) -CH2-R3 in the which X is H, CoA, glycerol, a monoglyceride, a diglyceride, ACP, methyl, Na +, phosphatidylcholine, or phosphatidylethanolamine, where both R1 and R2 are hydroxyl, one of R1 and R2 is hydroxyl and the other is H, or one of R1 and R2 is keto and the other is H, and where R3 is C2, C4, or C6 alkyl; and ii) XOC (O) -CH2-CH2-CH2-CH2-CH2-CH2-CH2-C = C-CH2-C- (O) -C-CH2-R3 in which X is H, CoA, glycerol, a monoglyceride, a diglyceride, ACP, methyl, Na +, phosphatidylcholine, or phosphatidylethanolamine, and where R3 is C2, C4, or C6 alkyl; and (b) a regulatory element linked functionally to said nucleic acid encoding said polypeptide, wherein said regulatory element confers expression in a vegetative tissue of said plant. Claim 115: The plant of claim 114, wherein said hydroxylated fatty acid is ricinileic acid. Claim 117: The plant of claim 116, wherein said epoxidized fatty acid is vernolic acid.

ARP050103261A 2004-08-04 2005-08-04 NUCLEIC ACIDS THAT CODIFY ANTIHELMINTIC AGENTS AND PLANTS OBTAINED FROM THESE. AR050201A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10/912,534 US7368629B2 (en) 2004-02-04 2004-08-04 Nucleic acids encoding anthelmintic agents and plants made therefrom

Publications (1)

Publication Number Publication Date
AR050201A1 true AR050201A1 (en) 2006-10-04

Family

ID=35839881

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP050103261A AR050201A1 (en) 2004-08-04 2005-08-04 NUCLEIC ACIDS THAT CODIFY ANTIHELMINTIC AGENTS AND PLANTS OBTAINED FROM THESE.

Country Status (12)

Country Link
US (4) US7368629B2 (en)
EP (2) EP1773110A4 (en)
JP (1) JP2008508877A (en)
CN (1) CN101076588A (en)
AR (1) AR050201A1 (en)
AU (1) AU2005271476A1 (en)
BR (1) BRPI0513080A (en)
CA (1) CA2575748A1 (en)
IL (6) IL181103A0 (en)
MX (1) MX2007001346A (en)
WO (1) WO2006017577A2 (en)
ZA (1) ZA200700960B (en)

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US7368629B2 (en) 2004-02-04 2008-05-06 Divergence, Inc. Nucleic acids encoding anthelmintic agents and plants made therefrom
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US8431772B1 (en) 2008-11-19 2013-04-30 University Of Kentucky Research Foundation Diacylglycerol acyltransferase sequences and related methods
WO2012071439A1 (en) * 2010-11-22 2012-05-31 The Regents Of The University Of California Host cells and methods for producing diacid compounds
AU2013255080A1 (en) * 2012-04-30 2014-12-18 Commonwealth Scientific And Industrial Research Organisation Fatty acid modification and tag assembly genes
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CN116530497A (en) * 2018-01-12 2023-08-04 吉武亨将 Method for removing harmful organisms, and method and apparatus for producing hypochlorous acid aqueous solution

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