AR042538A1 - Metodo para produccion de acidos grasos poliinsaturados - Google Patents

Metodo para produccion de acidos grasos poliinsaturados

Info

Publication number
AR042538A1
AR042538A1 ARP030104718A ARP030104718A AR042538A1 AR 042538 A1 AR042538 A1 AR 042538A1 AR P030104718 A ARP030104718 A AR P030104718A AR P030104718 A ARP030104718 A AR P030104718A AR 042538 A1 AR042538 A1 AR 042538A1
Authority
AR
Argentina
Prior art keywords
delta
nucleic acid
acid sequence
desaturase
encoding
Prior art date
Application number
ARP030104718A
Other languages
English (en)
Inventor
B Qi
E Heinz
T Zank
U Zaehringer
J A Napier
O Sayanova
C M Lazarus
Original Assignee
Univ Bristol
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0229578.0A external-priority patent/GB0229578D0/en
Priority claimed from GB0316989A external-priority patent/GB0316989D0/en
Application filed by Univ Bristol filed Critical Univ Bristol
Publication of AR042538A1 publication Critical patent/AR042538A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fats And Perfumes (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Saccharide Compounds (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Un procedimiento para la producción de compuestos de la fórmula general (1), en organismos transgénicos con un contenido de por lo menos 1% en peso de dichos compuestos respecto al contenido de lípidos total de dicho organismo, que comprende las etapas siguientes: a) introducción de por lo menos una secuencia de ácido nucleico en un organismo transgénico codificando a una delta-9-elongasa, y b) introducción de por lo menos una segunda secuencia de ácido nucleico codificando a una delta-8-desaturasa, y c) si es necesario, se introduce por lo menos una tercer secuencia de ácido nucleico codificando a una delta-5-desaturasa, y d) cultivo y recolección de dicho organismo; y en donde las variables y sustituyentes en la fórmula (1) poseen los significados siguientes: R1 = hidroxilo, Coenzima A-(Tioéster) fosfatidilcolina, fosfatidiletanolamina, fosfatidilglicerol, difosfatidilglicerol, fosfadilserina, fosfatidilinositol, esfingolípido, glicoshingolípido o un residuo de la fórmula general (2), R2 = hidrógeno, fosfatidilcolina, fosfatidiletanolamina, fosfatidilglierol, difosfatidilglierol, fosfadilserina, fosfatidilinositol, esingolípido, glicoshingolípido o un alquilamino C2-24 saturado o no saturado, R3 = hidrógeno, alquilcarbonilo C2-24 saturado o R2 y R3 independientemente entre sí son un residuo de la fórmula (3), donde n = 3, 4 ó 6, m = 3, 4 ó 5 y p = 0 ó 3, preferentemente = 3, m = 4 ó 5 y p = 0 ó 3. Secuencia de ácido nucleico específicas codificando proteínas con actividad delta-8-desaturasa, delta-9-elongasa o delta-desaturasa, constructos de ácido nucleico, vectores y organismos conteniendo dichas secuencias de ácido nucleico.
ARP030104718A 2002-12-19 2003-12-18 Metodo para produccion de acidos grasos poliinsaturados AR042538A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0229578.0A GB0229578D0 (en) 2002-12-19 2002-12-19 Novel method for the production of polyunsaturated fatty acids
GB0316989A GB0316989D0 (en) 2003-07-21 2003-07-21 Novel method for the production of polyunsaturated fatty acids

Publications (1)

Publication Number Publication Date
AR042538A1 true AR042538A1 (es) 2005-06-22

Family

ID=32683975

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP030104718A AR042538A1 (es) 2002-12-19 2003-12-18 Metodo para produccion de acidos grasos poliinsaturados

Country Status (12)

Country Link
US (1) US7714185B2 (es)
EP (1) EP1576166B1 (es)
JP (1) JP4781817B2 (es)
AR (1) AR042538A1 (es)
AU (1) AU2003296638B2 (es)
BR (1) BR0317304A (es)
CA (1) CA2510462A1 (es)
CL (1) CL2003002667A1 (es)
ES (1) ES2525212T3 (es)
IL (2) IL168783A (es)
MX (1) MXPA05005777A (es)
WO (1) WO2004057001A2 (es)

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CA2510462A1 (en) 2004-07-08
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CL2003002667A1 (es) 2005-04-08
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EP1576166A2 (en) 2005-09-21
WO2004057001A3 (en) 2004-11-04
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JP4781817B2 (ja) 2011-09-28
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