AR034413A1 - Metodo para determinar la actividad de las enzimas lisosomales - Google Patents

Metodo para determinar la actividad de las enzimas lisosomales

Info

Publication number
AR034413A1
AR034413A1 ARP010104070A ARP010104070A AR034413A1 AR 034413 A1 AR034413 A1 AR 034413A1 AR P010104070 A ARP010104070 A AR P010104070A AR P010104070 A ARP010104070 A AR P010104070A AR 034413 A1 AR034413 A1 AR 034413A1
Authority
AR
Argentina
Prior art keywords
alpha
beta
activity
galactosidase
lysosomal
Prior art date
Application number
ARP010104070A
Other languages
English (en)
Inventor
Nestor Abel Chamoles
Original Assignee
Nestor Abel Chamoles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nestor Abel Chamoles filed Critical Nestor Abel Chamoles
Publication of AR034413A1 publication Critical patent/AR034413A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

Abstract

Un método para determinar la actividad de las enzimas lisosomales presentes en muestras secas de fluidos corporales y de tejidos celulares tales como alfa-L-iduronidasa, beta-D-galactosidasa, beta-D-glucosidasa, citotriosidasa, alfa-D-galactosidasa total y alfa-D-galactosidasa A, hexosaminidasa A y B, alfa-D-manosidasa, beta-D-manosidasa, alfa-L-fucosidasa, N-acetil-alfa-galactosaminidasa, arilsulfatasa, esfingomielinasa, beta-galactocerebrosidasa, iduronato-2-sulfatasa y beta-D-glucuronidasa, entre otras. El método incluye: a) combinar una muestra seca de fluidos corporales o tejidos celulares conteniendo por lo menos un tipo de enzima lisosomal con: 1) un eluyente, 2) una solución amortiguadora de incubación y 3) un sustrato o sustratos capaces de reaccionar con las enzimas lisosomales ensayadas y de producir un producto enzimático correspondiente, b) permitir que la muestra seca de fluido corporal o tejido celular reaccione con el eluyente, la solución amortiguadora de incubación y el sustrato o sustratos durante un tiempo y temperatura adecuados, y c) detectar la mencionada enzima lisosomal. La presente también se refiere a kit de diagnóstico para la determinación de la actividad de las enzimas lisosomales.
ARP010104070A 2000-08-25 2001-08-24 Metodo para determinar la actividad de las enzimas lisosomales AR034413A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22757300P 2000-08-25 2000-08-25
PCT/US2001/026259 WO2002018539A2 (en) 2000-08-25 2001-08-24 Method for assaying the activity of lysosomal enzymes

Publications (1)

Publication Number Publication Date
AR034413A1 true AR034413A1 (es) 2004-02-25

Family

ID=22853625

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010104070A AR034413A1 (es) 2000-08-25 2001-08-24 Metodo para determinar la actividad de las enzimas lisosomales

Country Status (4)

Country Link
US (2) US7563591B2 (es)
AR (1) AR034413A1 (es)
AU (1) AU2001286636A1 (es)
WO (1) WO2002018539A2 (es)

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CA2661789C (en) * 2006-04-07 2014-06-03 The Scripps Research Institute Modified .alpha.-galactosyl ceramides for staining and stimulating natural killer t cells
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AU2017206687B2 (en) * 2016-01-14 2021-12-23 Jamlet MONASELIDZE Differential scanning microcalorimeter device for detecting disease and monitoring therapeutic efficacy
CN106282231B (zh) * 2016-09-06 2020-01-03 陕西慧康生物科技有限责任公司 粘多糖贮积症ii型动物模型的构建方法及应用
CN107014934B (zh) * 2017-04-14 2020-12-08 广州金域医学检验中心有限公司 尿液有机酸滤纸片质控品及其制备方法
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Also Published As

Publication number Publication date
WO2002018539A3 (en) 2002-07-18
US20040166554A1 (en) 2004-08-26
US7563591B2 (en) 2009-07-21
AU2001286636A1 (en) 2002-03-13
WO2002018539A2 (en) 2002-03-07
US20090325206A1 (en) 2009-12-31

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