ZA202204756B - Compositions and methods for rna-encoded dna-replacement of alleles - Google Patents
Compositions and methods for rna-encoded dna-replacement of allelesInfo
- Publication number
- ZA202204756B ZA202204756B ZA2022/04756A ZA202204756A ZA202204756B ZA 202204756 B ZA202204756 B ZA 202204756B ZA 2022/04756 A ZA2022/04756 A ZA 2022/04756A ZA 202204756 A ZA202204756 A ZA 202204756A ZA 202204756 B ZA202204756 B ZA 202204756B
- Authority
- ZA
- South Africa
- Prior art keywords
- type
- extended
- crispr
- nucleic acid
- methods
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 108700028369 Alleles Proteins 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 abstract 6
- 102000039446 nucleic acids Human genes 0.000 abstract 6
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 239000012636 effector Substances 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 102100034343 Integrase Human genes 0.000 abstract 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 2
- 108091033409 CRISPR Proteins 0.000 abstract 1
- 108091079001 CRISPR RNA Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/85—Fusion polypeptide containing an RNA binding domain
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962930836P | 2019-11-05 | 2019-11-05 | |
| PCT/US2020/059045 WO2021092130A1 (en) | 2019-11-05 | 2020-11-05 | Compositions and methods for rna-encoded dna-replacement of alleles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA202204756B true ZA202204756B (en) | 2024-10-30 |
Family
ID=75687119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA2022/04756A ZA202204756B (en) | 2019-11-05 | 2022-04-28 | Compositions and methods for rna-encoded dna-replacement of alleles |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US11926834B2 (https=) |
| EP (1) | EP4054621A4 (https=) |
| JP (1) | JP7785002B2 (https=) |
| KR (1) | KR20220110748A (https=) |
| CN (1) | CN114901302A (https=) |
| AU (1) | AU2020380319A1 (https=) |
| BR (1) | BR112022008415A2 (https=) |
| CA (1) | CA3160186A1 (https=) |
| CL (1) | CL2022001145A1 (https=) |
| IL (1) | IL292434A (https=) |
| MX (1) | MX2022005328A (https=) |
| NZ (1) | NZ787446A (https=) |
| WO (1) | WO2021092130A1 (https=) |
| ZA (1) | ZA202204756B (https=) |
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| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| AU2020369599A1 (en) | 2019-10-23 | 2022-05-12 | Monsanto Technology Llc | Compositions and methods for RNA-templated editing in plants |
| CA3160186A1 (en) | 2019-11-05 | 2021-05-14 | Pairwise Plants Services, Inc. | Compositions and methods for rna-encoded dna-replacement of alleles |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| US20220145334A1 (en) * | 2020-11-06 | 2022-05-12 | Pairwise Plants Services, Inc. | Compositions and methods for rna-encoded dna-replacement of alleles |
| EP4347818A2 (en) * | 2021-06-01 | 2024-04-10 | Arbor Biotechnologies, Inc. | Gene editing systems comprising a crispr nuclease and uses thereof |
| US20220403475A1 (en) | 2021-06-14 | 2022-12-22 | Pairwise Plants Services, Inc. | Reporter constructs, compositions comprising the same, and methods of use thereof |
| AU2022343268A1 (en) | 2021-09-08 | 2024-03-28 | Flagship Pioneering Innovations Vi, Llc | Methods and compositions for modulating a genome |
| WO2023049728A1 (en) | 2021-09-21 | 2023-03-30 | Pairwise Plants Services, Inc. | Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same |
| EP4596696A3 (en) | 2021-09-28 | 2025-08-27 | Acrigen Biosciences | Compositions and methods for nucleic acid modifications |
| WO2023102329A2 (en) * | 2021-11-30 | 2023-06-08 | Mammoth Biosciences, Inc. | Effector proteins and uses thereof |
| CN114317492A (zh) * | 2021-12-06 | 2022-04-12 | 北京大学 | 一种改造的人工核酸酶系统及其应用 |
| CN116064512A (zh) * | 2022-07-15 | 2023-05-05 | 山西大学 | 一种改进的引导编辑系统及其应用 |
| JP2025525569A (ja) * | 2022-07-18 | 2025-08-05 | レナゲード セラピューティクス マネージメント インコーポレイテッド | 遺伝子編集成分、システム、及び使用方法 |
| US12264328B2 (en) | 2022-07-18 | 2025-04-01 | ReNAgade Therapeutics Management, Inc. | Gene editing components, systems, and methods of use |
| CA3268411A1 (en) * | 2022-09-23 | 2024-03-28 | Flagship Pioneering Innovations Vi, Llc | METHODS FOR ENRICHING TARGET NUCLEIC ACID SEQUENCES |
| WO2024096790A1 (en) * | 2022-10-31 | 2024-05-10 | Soledits Ab | Modular crispr system |
| EP4627083A1 (en) * | 2022-11-30 | 2025-10-08 | Arbor Biotechnologies, Inc. | Reverse transcriptase-mediated genetic editing of transthyretin (ttr) and uses thereof |
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| EP4634375A2 (en) | 2022-12-16 | 2025-10-22 | Pairwise Plants Services, Inc. | Fusion proteins comprising a cas12a polypeptide and an intein and methods of use therof |
| US20240271109A1 (en) | 2022-12-21 | 2024-08-15 | Pairwise Plants Services, Inc. | Engineered proteins and methods of use thereof |
| AU2024239266A1 (en) | 2023-03-20 | 2025-09-25 | Pioneer Hi-Bred International, Inc. | Cas polypeptides with altered pam recognition |
| AU2024348958A1 (en) | 2023-09-26 | 2026-04-02 | Pairwise Plants Services, Inc. | Fusion proteins, compositions comprising the same, and methods of use thereof |
| AU2024357941A1 (en) | 2023-10-09 | 2026-04-02 | Pairwise Plants Services, Inc. | Circular permutants, compositions comprising the same, and methods of use thereof |
| WO2026019871A1 (en) | 2024-07-17 | 2026-01-22 | Pairwise Plants Services, Inc. | Engineered cytidine deaminases |
| US20260022361A1 (en) | 2024-07-17 | 2026-01-22 | Pairwise Plants Services, Inc. | Engineered proteins and methods of use thereof |
| US12297441B1 (en) | 2024-09-11 | 2025-05-13 | Pairwise Plants Services, Inc. | Methods and compositions for modifying endocarp structure in Rubus plants |
| WO2026085100A1 (en) | 2024-10-15 | 2026-04-23 | Pairwise Plants Services, Inc. | Methods for high density planting of rubus plants comprising a mutation in the terminal flower (tfl) gene |
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| US20220145334A1 (en) | 2020-11-06 | 2022-05-12 | Pairwise Plants Services, Inc. | Compositions and methods for rna-encoded dna-replacement of alleles |
-
2020
- 2020-11-05 CA CA3160186A patent/CA3160186A1/en active Pending
- 2020-11-05 US US17/090,334 patent/US11926834B2/en active Active
- 2020-11-05 NZ NZ787446A patent/NZ787446A/en unknown
- 2020-11-05 EP EP20884787.1A patent/EP4054621A4/en active Pending
- 2020-11-05 WO PCT/US2020/059045 patent/WO2021092130A1/en not_active Ceased
- 2020-11-05 BR BR112022008415A patent/BR112022008415A2/pt unknown
- 2020-11-05 IL IL292434A patent/IL292434A/en unknown
- 2020-11-05 AU AU2020380319A patent/AU2020380319A1/en active Pending
- 2020-11-05 CN CN202080084407.0A patent/CN114901302A/zh active Pending
- 2020-11-05 JP JP2022538683A patent/JP7785002B2/ja active Active
- 2020-11-05 MX MX2022005328A patent/MX2022005328A/es unknown
- 2020-11-05 KR KR1020227018853A patent/KR20220110748A/ko active Pending
-
2022
- 2022-04-28 ZA ZA2022/04756A patent/ZA202204756B/en unknown
- 2022-05-03 CL CL2022001145A patent/CL2022001145A1/es unknown
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2024
- 2024-01-26 US US18/423,374 patent/US20240167045A1/en active Pending
- 2024-01-26 US US18/423,378 patent/US20240158804A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2020380319A1 (en) | 2022-05-12 |
| CN114901302A (zh) | 2022-08-12 |
| JP7785002B2 (ja) | 2025-12-12 |
| JP2023505600A (ja) | 2023-02-09 |
| US20240167045A1 (en) | 2024-05-23 |
| EP4054621A1 (en) | 2022-09-14 |
| US20240158804A1 (en) | 2024-05-16 |
| WO2021092130A1 (en) | 2021-05-14 |
| US11926834B2 (en) | 2024-03-12 |
| EP4054621A4 (en) | 2023-12-27 |
| CL2022001145A1 (es) | 2023-02-03 |
| IL292434A (en) | 2022-06-01 |
| CA3160186A1 (en) | 2021-05-14 |
| NZ787446A (en) | 2026-02-27 |
| US20210130835A1 (en) | 2021-05-06 |
| BR112022008415A2 (pt) | 2022-09-06 |
| KR20220110748A (ko) | 2022-08-09 |
| MX2022005328A (es) | 2022-07-21 |
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