IL292434A - Compositions and methods for rna-encoded dna-replacement of alleles - Google Patents
Compositions and methods for rna-encoded dna-replacement of allelesInfo
- Publication number
- IL292434A IL292434A IL292434A IL29243422A IL292434A IL 292434 A IL292434 A IL 292434A IL 292434 A IL292434 A IL 292434A IL 29243422 A IL29243422 A IL 29243422A IL 292434 A IL292434 A IL 292434A
- Authority
- IL
- Israel
- Prior art keywords
- crispr
- type
- nucleic acid
- cas effector
- effector protein
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 145
- 108700028369 Alleles Proteins 0.000 title claims description 9
- 238000002360 preparation method Methods 0.000 title description 2
- 150000007523 nucleic acids Chemical class 0.000 claims description 462
- 102000039446 nucleic acids Human genes 0.000 claims description 453
- 108020004707 nucleic acids Proteins 0.000 claims description 453
- 108090000623 proteins and genes Proteins 0.000 claims description 308
- 239000012636 effector Substances 0.000 claims description 306
- 102000004169 proteins and genes Human genes 0.000 claims description 281
- 239000002773 nucleotide Substances 0.000 claims description 239
- 125000003729 nucleotide group Chemical group 0.000 claims description 239
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 236
- 102100031780 Endonuclease Human genes 0.000 claims description 217
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 217
- 230000014509 gene expression Effects 0.000 claims description 176
- 102000040430 polynucleotide Human genes 0.000 claims description 152
- 108091033319 polynucleotide Proteins 0.000 claims description 152
- 239000002157 polynucleotide Substances 0.000 claims description 152
- 229920001184 polypeptide Polymers 0.000 claims description 148
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 148
- 241000196324 Embryophyta Species 0.000 claims description 142
- 102000037865 fusion proteins Human genes 0.000 claims description 113
- 108020001507 fusion proteins Proteins 0.000 claims description 113
- 230000027455 binding Effects 0.000 claims description 94
- 108020004414 DNA Proteins 0.000 claims description 90
- 108060002716 Exonuclease Proteins 0.000 claims description 72
- 102000013165 exonuclease Human genes 0.000 claims description 72
- 108091079001 CRISPR RNA Proteins 0.000 claims description 68
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 63
- 125000006850 spacer group Chemical group 0.000 claims description 60
- 108090000652 Flap endonucleases Proteins 0.000 claims description 59
- 102000004150 Flap endonucleases Human genes 0.000 claims description 59
- 230000000295 complement effect Effects 0.000 claims description 59
- 108091033409 CRISPR Proteins 0.000 claims description 55
- 101710163270 Nuclease Proteins 0.000 claims description 52
- 239000013598 vector Substances 0.000 claims description 51
- 230000035772 mutation Effects 0.000 claims description 49
- 108020001580 protein domains Proteins 0.000 claims description 47
- 230000000694 effects Effects 0.000 claims description 45
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 38
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 38
- 101150088049 dna2 gene Proteins 0.000 claims description 36
- 108020004705 Codon Proteins 0.000 claims description 33
- -1 extended CRISPR RNA Chemical class 0.000 claims description 29
- 108020005004 Guide RNA Proteins 0.000 claims description 27
- 150000001413 amino acids Chemical class 0.000 claims description 23
- 230000006780 non-homologous end joining Effects 0.000 claims description 18
- 230000033607 mismatch repair Effects 0.000 claims description 15
- 102000004190 Enzymes Human genes 0.000 claims description 14
- 108090000790 Enzymes Proteins 0.000 claims description 14
- 230000003993 interaction Effects 0.000 claims description 13
- 239000000126 substance Substances 0.000 claims description 13
- 102000053602 DNA Human genes 0.000 claims description 12
- 241000209510 Liliopsida Species 0.000 claims description 11
- 108020004999 messenger RNA Proteins 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 claims description 10
- 101710125418 Major capsid protein Proteins 0.000 claims description 10
- 238000012545 processing Methods 0.000 claims description 10
- 241001465754 Metazoa Species 0.000 claims description 9
- 230000005782 double-strand break Effects 0.000 claims description 9
- 230000002829 reductive effect Effects 0.000 claims description 9
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 8
- 241001233957 eudicotyledons Species 0.000 claims description 8
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims description 7
- 241000233866 Fungi Species 0.000 claims description 7
- 102000009572 RNA Polymerase II Human genes 0.000 claims description 7
- 108010009460 RNA Polymerase II Proteins 0.000 claims description 7
- 241000617156 archaeon Species 0.000 claims description 7
- 101710132601 Capsid protein Proteins 0.000 claims description 6
- 101710094648 Coat protein Proteins 0.000 claims description 6
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 claims description 6
- 101710141454 Nucleoprotein Proteins 0.000 claims description 6
- 101710083689 Probable capsid protein Proteins 0.000 claims description 6
- 108091028113 Trans-activating crRNA Proteins 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 230000008685 targeting Effects 0.000 claims description 6
- 230000004927 fusion Effects 0.000 claims description 5
- 108010011170 Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly Proteins 0.000 claims description 4
- 108020004682 Single-Stranded DNA Proteins 0.000 claims description 4
- 108020004459 Small interfering RNA Proteins 0.000 claims description 4
- 108010017842 Telomerase Proteins 0.000 claims description 4
- 239000013000 chemical inhibitor Substances 0.000 claims description 4
- 101150014310 fem-3 gene Proteins 0.000 claims description 4
- 108010055863 gene b exonuclease Proteins 0.000 claims description 4
- 238000003197 gene knockdown Methods 0.000 claims description 4
- 239000003446 ligand Substances 0.000 claims description 4
- 238000011144 upstream manufacturing Methods 0.000 claims description 4
- 108091023037 Aptamer Proteins 0.000 claims description 3
- 230000007018 DNA scission Effects 0.000 claims description 3
- 108700022150 Designed Ankyrin Repeat Proteins Proteins 0.000 claims description 3
- 230000004570 RNA-binding Effects 0.000 claims description 3
- 230000004850 protein–protein interaction Effects 0.000 claims description 3
- 230000001172 regenerating effect Effects 0.000 claims description 3
- 101710159080 Aconitate hydratase A Proteins 0.000 claims description 2
- 101710159078 Aconitate hydratase B Proteins 0.000 claims description 2
- 101100107610 Arabidopsis thaliana ABCF4 gene Proteins 0.000 claims description 2
- 102000015335 Ku Autoantigen Human genes 0.000 claims description 2
- 108010025026 Ku Autoantigen Proteins 0.000 claims description 2
- 108091008103 RNA aptamers Proteins 0.000 claims description 2
- 102000044126 RNA-Binding Proteins Human genes 0.000 claims description 2
- 101710105008 RNA-binding protein Proteins 0.000 claims description 2
- 101100068078 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GCN4 gene Proteins 0.000 claims description 2
- 102000018679 Tacrolimus Binding Proteins Human genes 0.000 claims description 2
- 108010027179 Tacrolimus Binding Proteins Proteins 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 230000008901 benefit Effects 0.000 claims description 2
- 230000001588 bifunctional effect Effects 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000006471 dimerization reaction Methods 0.000 claims description 2
- 125000001475 halogen functional group Chemical group 0.000 claims description 2
- 239000000833 heterodimer Substances 0.000 claims description 2
- 238000005457 optimization Methods 0.000 claims 4
- 238000010354 CRISPR gene editing Methods 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 description 199
- 210000004027 cell Anatomy 0.000 description 110
- 239000013612 plasmid Substances 0.000 description 82
- 101150067314 aadA gene Proteins 0.000 description 58
- 230000009466 transformation Effects 0.000 description 29
- 108091028043 Nucleic acid sequence Proteins 0.000 description 27
- 240000008042 Zea mays Species 0.000 description 20
- 230000006870 function Effects 0.000 description 20
- 108700004991 Cas12a Proteins 0.000 description 19
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 18
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 17
- 230000001105 regulatory effect Effects 0.000 description 17
- 101150098466 rpsL gene Proteins 0.000 description 17
- 238000009396 hybridization Methods 0.000 description 15
- 210000001519 tissue Anatomy 0.000 description 15
- 238000013461 design Methods 0.000 description 14
- 101150070420 gyrA gene Proteins 0.000 description 14
- 241000588724 Escherichia coli Species 0.000 description 13
- 239000003550 marker Substances 0.000 description 13
- 238000007480 sanger sequencing Methods 0.000 description 13
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 12
- 235000001014 amino acid Nutrition 0.000 description 12
- 229940024606 amino acid Drugs 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 12
- 235000009973 maize Nutrition 0.000 description 12
- 108091092195 Intron Proteins 0.000 description 11
- 239000003242 anti bacterial agent Substances 0.000 description 10
- 108090000848 Ubiquitin Proteins 0.000 description 9
- 230000003115 biocidal effect Effects 0.000 description 9
- 230000010076 replication Effects 0.000 description 9
- 229960005322 streptomycin Drugs 0.000 description 9
- 238000013518 transcription Methods 0.000 description 9
- 230000035897 transcription Effects 0.000 description 9
- 101100342977 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) leu-1 gene Proteins 0.000 description 8
- 102000044159 Ubiquitin Human genes 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 8
- 239000003623 enhancer Substances 0.000 description 8
- 230000001976 improved effect Effects 0.000 description 8
- 230000004048 modification Effects 0.000 description 8
- 238000012986 modification Methods 0.000 description 8
- 238000003752 polymerase chain reaction Methods 0.000 description 8
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 8
- 229940088710 antibiotic agent Drugs 0.000 description 7
- 239000012634 fragment Substances 0.000 description 7
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 7
- 230000001965 increasing effect Effects 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 108091026890 Coding region Proteins 0.000 description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 6
- 241000282414 Homo sapiens Species 0.000 description 6
- 108700019146 Transgenes Proteins 0.000 description 6
- 238000003556 assay Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 244000241257 Cucumis melo Species 0.000 description 5
- 235000010469 Glycine max Nutrition 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 5
- 241000713869 Moloney murine leukemia virus Species 0.000 description 5
- 240000007594 Oryza sativa Species 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 230000001413 cellular effect Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 5
- 229920002401 polyacrylamide Polymers 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 101150018129 CSF2 gene Proteins 0.000 description 4
- 101150069031 CSN2 gene Proteins 0.000 description 4
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
- 101100494762 Mus musculus Nedd9 gene Proteins 0.000 description 4
- 101100385413 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) csm-3 gene Proteins 0.000 description 4
- 235000010582 Pisum sativum Nutrition 0.000 description 4
- 240000004713 Pisum sativum Species 0.000 description 4
- 235000007244 Zea mays Nutrition 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 238000007792 addition Methods 0.000 description 4
- 235000004279 alanine Nutrition 0.000 description 4
- 238000013459 approach Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 101150055601 cops2 gene Proteins 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- 239000012467 final product Substances 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 229930027917 kanamycin Natural products 0.000 description 4
- 229960000318 kanamycin Drugs 0.000 description 4
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 4
- 229930182823 kanamycin A Natural products 0.000 description 4
- 230000030648 nucleus localization Effects 0.000 description 4
- 235000014571 nuts Nutrition 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 229940113082 thymine Drugs 0.000 description 4
- 230000002103 transcriptional effect Effects 0.000 description 4
- 244000291564 Allium cepa Species 0.000 description 3
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 3
- 241000219194 Arabidopsis Species 0.000 description 3
- 241001672694 Citrus reticulata Species 0.000 description 3
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 description 3
- 235000009854 Cucurbita moschata Nutrition 0.000 description 3
- 240000001980 Cucurbita pepo Species 0.000 description 3
- 239000004471 Glycine Substances 0.000 description 3
- 244000020551 Helianthus annuus Species 0.000 description 3
- 235000003222 Helianthus annuus Nutrition 0.000 description 3
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 3
- 239000006142 Luria-Bertani Agar Substances 0.000 description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
- 241000219823 Medicago Species 0.000 description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 3
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 3
- 240000003768 Solanum lycopersicum Species 0.000 description 3
- 244000062793 Sorghum vulgare Species 0.000 description 3
- 108091027544 Subgenomic mRNA Proteins 0.000 description 3
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 229960005091 chloramphenicol Drugs 0.000 description 3
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 3
- 210000003763 chloroplast Anatomy 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000010362 genome editing Methods 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 230000000977 initiatory effect Effects 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 240000004308 marijuana Species 0.000 description 3
- 230000002438 mitochondrial effect Effects 0.000 description 3
- 229960000210 nalidixic acid Drugs 0.000 description 3
- MHWLWQUZZRMNGJ-UHFFFAOYSA-N nalidixic acid Chemical compound C1=C(C)N=C2N(CC)C=C(C(O)=O)C(=O)C2=C1 MHWLWQUZZRMNGJ-UHFFFAOYSA-N 0.000 description 3
- 210000002706 plastid Anatomy 0.000 description 3
- 230000037452 priming Effects 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 238000010839 reverse transcription Methods 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 230000009261 transgenic effect Effects 0.000 description 3
- 230000001052 transient effect Effects 0.000 description 3
- 239000013603 viral vector Substances 0.000 description 3
- LWTDZKXXJRRKDG-KXBFYZLASA-N (-)-phaseollin Chemical compound C1OC2=CC(O)=CC=C2[C@H]2[C@@H]1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-KXBFYZLASA-N 0.000 description 2
- FVFVNNKYKYZTJU-UHFFFAOYSA-N 6-chloro-1,3,5-triazine-2,4-diamine Chemical compound NC1=NC(N)=NC(Cl)=N1 FVFVNNKYKYZTJU-UHFFFAOYSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 2
- 108010085238 Actins Proteins 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 241000203069 Archaea Species 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 235000016068 Berberis vulgaris Nutrition 0.000 description 2
- 241000335053 Beta vulgaris Species 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 241000167854 Bourreria succulenta Species 0.000 description 2
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 2
- 240000000385 Brassica napus var. napus Species 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 2
- 235000012905 Brassica oleracea var viridis Nutrition 0.000 description 2
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 2
- 244000221633 Brassica rapa subsp chinensis Species 0.000 description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 2
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 2
- 108090000209 Carbonic anhydrases Proteins 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 2
- 241000747028 Cestrum yellow leaf curling virus Species 0.000 description 2
- 240000006740 Cichorium endivia Species 0.000 description 2
- 244000241235 Citrullus lanatus Species 0.000 description 2
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 2
- 241000218631 Coniferophyta Species 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 241000699800 Cricetinae Species 0.000 description 2
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 2
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 2
- 235000009852 Cucurbita pepo Nutrition 0.000 description 2
- 230000004568 DNA-binding Effects 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 241000283086 Equidae Species 0.000 description 2
- 241000283074 Equus asinus Species 0.000 description 2
- 241000282326 Felis catus Species 0.000 description 2
- 241000699694 Gerbillinae Species 0.000 description 2
- 241000282575 Gorilla Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 241000218922 Magnoliophyta Species 0.000 description 2
- 101100113998 Mus musculus Cnbd2 gene Proteins 0.000 description 2
- 241000282339 Mustela Species 0.000 description 2
- 108090000913 Nitrate Reductases Proteins 0.000 description 2
- 238000000636 Northern blotting Methods 0.000 description 2
- 108091005461 Nucleic proteins Proteins 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 241000282577 Pan troglodytes Species 0.000 description 2
- 241001504519 Papio ursinus Species 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 108091000041 Phosphoenolpyruvate Carboxylase Proteins 0.000 description 2
- 240000006711 Pistacia vera Species 0.000 description 2
- 241000288906 Primates Species 0.000 description 2
- 241001092459 Rubus Species 0.000 description 2
- 240000007651 Rubus glaucus Species 0.000 description 2
- 235000003942 Rubus occidentalis Nutrition 0.000 description 2
- 244000111388 Rubus occidentalis Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- 241000193996 Streptococcus pyogenes Species 0.000 description 2
- 108010043934 Sucrose synthase Proteins 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 108091036066 Three prime untranslated region Proteins 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 244000098338 Triticum aestivum Species 0.000 description 2
- 229920002494 Zein Polymers 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 235000021029 blackberry Nutrition 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000000981 bystander Effects 0.000 description 2
- 235000005607 chanvre indien Nutrition 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 235000019693 cherries Nutrition 0.000 description 2
- 235000003733 chicria Nutrition 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 229940104302 cytosine Drugs 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 229920000140 heteropolymer Polymers 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 239000000543 intermediate Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000002679 microRNA Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 235000020233 pistachio Nutrition 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000007115 recruitment Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 229960000268 spectinomycin Drugs 0.000 description 2
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 2
- 235000020354 squash Nutrition 0.000 description 2
- 241001515965 unidentified phage Species 0.000 description 2
- 239000005019 zein Substances 0.000 description 2
- 229940093612 zein Drugs 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- 108010052418 (N-(2-((4-((2-((4-(9-acridinylamino)phenyl)amino)-2-oxoethyl)amino)-4-oxobutyl)amino)-1-(1H-imidazol-4-ylmethyl)-1-oxoethyl)-6-(((-2-aminoethyl)amino)methyl)-2-pyridinecarboxamidato) iron(1+) Proteins 0.000 description 1
- DSSYKIVIOFKYAU-XCBNKYQSSA-N (R)-camphor Chemical compound C1C[C@@]2(C)C(=O)C[C@@H]1C2(C)C DSSYKIVIOFKYAU-XCBNKYQSSA-N 0.000 description 1
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
- 108020003589 5' Untranslated Regions Proteins 0.000 description 1
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 description 1
- CKOMXBHMKXXTNW-UHFFFAOYSA-N 6-methyladenine Chemical compound CNC1=NC=NC2=C1N=CN2 CKOMXBHMKXXTNW-UHFFFAOYSA-N 0.000 description 1
- 240000004507 Abelmoschus esculentus Species 0.000 description 1
- 241000208140 Acer Species 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 101710197633 Actin-1 Proteins 0.000 description 1
- 235000009434 Actinidia chinensis Nutrition 0.000 description 1
- 244000298697 Actinidia deliciosa Species 0.000 description 1
- 235000009436 Actinidia deliciosa Nutrition 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 101710146995 Acyl carrier protein Proteins 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 241000743339 Agrostis Species 0.000 description 1
- 101710187578 Alcohol dehydrogenase 1 Proteins 0.000 description 1
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 description 1
- 235000005254 Allium ampeloprasum Nutrition 0.000 description 1
- 240000006108 Allium ampeloprasum Species 0.000 description 1
- 235000010167 Allium cepa var aggregatum Nutrition 0.000 description 1
- 240000002234 Allium sativum Species 0.000 description 1
- 101100301006 Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) cbbL2 gene Proteins 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 244000144725 Amygdalus communis Species 0.000 description 1
- 244000099147 Ananas comosus Species 0.000 description 1
- 235000007119 Ananas comosus Nutrition 0.000 description 1
- 108020003566 Antisense Oligodeoxyribonucleotides Proteins 0.000 description 1
- 235000003276 Apios tuberosa Nutrition 0.000 description 1
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000010744 Arachis villosulicarpa Nutrition 0.000 description 1
- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 241001494510 Arundo Species 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 235000000832 Ayote Nutrition 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 235000018185 Betula X alpestris Nutrition 0.000 description 1
- 235000018212 Betula X uliginosa Nutrition 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 235000003351 Brassica cretica Nutrition 0.000 description 1
- 235000011297 Brassica napobrassica Nutrition 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 241000219192 Brassica napus subsp. rapifera Species 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000004221 Brassica oleracea var gemmifera Nutrition 0.000 description 1
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 244000308368 Brassica oleracea var. gemmifera Species 0.000 description 1
- 244000304217 Brassica oleracea var. gongylodes Species 0.000 description 1
- 240000004073 Brassica oleracea var. viridis Species 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
- 235000003343 Brassica rupestris Nutrition 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 241000195940 Bryophyta Species 0.000 description 1
- 238000010356 CRISPR-Cas9 genome editing Methods 0.000 description 1
- 241000219357 Cactaceae Species 0.000 description 1
- 101100533230 Caenorhabditis elegans ser-2 gene Proteins 0.000 description 1
- 241000632195 Calamagrostis x acutiflora Species 0.000 description 1
- 235000008697 Cannabis sativa Nutrition 0.000 description 1
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 240000006432 Carica papaya Species 0.000 description 1
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 1
- 244000020518 Carthamus tinctorius Species 0.000 description 1
- 235000009025 Carya illinoensis Nutrition 0.000 description 1
- 244000068645 Carya illinoensis Species 0.000 description 1
- 235000014036 Castanea Nutrition 0.000 description 1
- 241001070941 Castanea Species 0.000 description 1
- 102000053642 Catalytic RNA Human genes 0.000 description 1
- 108090000994 Catalytic RNA Proteins 0.000 description 1
- 241000218645 Cedrus Species 0.000 description 1
- 244000146553 Ceiba pentandra Species 0.000 description 1
- 235000003301 Ceiba pentandra Nutrition 0.000 description 1
- 108010004539 Chalcone isomerase Proteins 0.000 description 1
- 235000021538 Chard Nutrition 0.000 description 1
- 240000006162 Chenopodium quinoa Species 0.000 description 1
- 235000007542 Cichorium intybus Nutrition 0.000 description 1
- 244000298479 Cichorium intybus Species 0.000 description 1
- 241000723346 Cinnamomum camphora Species 0.000 description 1
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000175448 Citrus madurensis Species 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 240000000560 Citrus x paradisi Species 0.000 description 1
- 235000013162 Cocos nucifera Nutrition 0.000 description 1
- 244000060011 Cocos nucifera Species 0.000 description 1
- 240000007154 Coffea arabica Species 0.000 description 1
- 235000006481 Colocasia esculenta Nutrition 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 240000000491 Corchorus aestuans Species 0.000 description 1
- 235000011777 Corchorus aestuans Nutrition 0.000 description 1
- 235000010862 Corchorus capsularis Nutrition 0.000 description 1
- 241000723382 Corylus Species 0.000 description 1
- 235000007466 Corylus avellana Nutrition 0.000 description 1
- 101710190853 Cruciferin Proteins 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 241000219130 Cucurbita pepo subsp. pepo Species 0.000 description 1
- 235000003954 Cucurbita pepo var melopepo Nutrition 0.000 description 1
- 244000301850 Cupressus sempervirens Species 0.000 description 1
- 235000017788 Cydonia oblonga Nutrition 0.000 description 1
- 244000019459 Cynara cardunculus Species 0.000 description 1
- 235000019106 Cynara scolymus Nutrition 0.000 description 1
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
- 102000011724 DNA Repair Enzymes Human genes 0.000 description 1
- 108010076525 DNA Repair Enzymes Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 241001522995 Deschampsia cespitosa Species 0.000 description 1
- 235000011511 Diospyros Nutrition 0.000 description 1
- 241000723267 Diospyros Species 0.000 description 1
- 235000001950 Elaeis guineensis Nutrition 0.000 description 1
- 244000127993 Elaeis melanococca Species 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 241000701832 Enterobacteria phage T3 Species 0.000 description 1
- 244000024675 Eruca sativa Species 0.000 description 1
- 235000014755 Eruca sativa Nutrition 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 244000004281 Eucalyptus maculata Species 0.000 description 1
- 235000009419 Fagopyrum esculentum Nutrition 0.000 description 1
- 240000008620 Fagopyrum esculentum Species 0.000 description 1
- 108010087894 Fatty acid desaturases Proteins 0.000 description 1
- 102000009114 Fatty acid desaturases Human genes 0.000 description 1
- 241000234642 Festuca Species 0.000 description 1
- 235000017317 Fortunella Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 101000860092 Francisella tularensis subsp. novicida (strain U112) CRISPR-associated endonuclease Cas12a Proteins 0.000 description 1
- 101710186901 Globulin 1 Proteins 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 101710115777 Glycine-rich cell wall structural protein 2 Proteins 0.000 description 1
- 101710168683 Glycine-rich protein 1 Proteins 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 241001091440 Grossulariaceae Species 0.000 description 1
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 1
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 208000009889 Herpes Simplex Diseases 0.000 description 1
- 244000043261 Hevea brasiliensis Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 1
- 235000008694 Humulus lupulus Nutrition 0.000 description 1
- 244000025221 Humulus lupulus Species 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 241000758791 Juglandaceae Species 0.000 description 1
- 241000256560 Kandleria Species 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 241000218195 Lauraceae Species 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 244000207740 Lemna minor Species 0.000 description 1
- 235000006439 Lemna minor Nutrition 0.000 description 1
- 240000004322 Lens culinaris Species 0.000 description 1
- 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 description 1
- 241000270322 Lepidosauria Species 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241001072282 Limnanthes Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 244000108452 Litchi chinensis Species 0.000 description 1
- 241000209082 Lolium Species 0.000 description 1
- 241000208467 Macadamia Species 0.000 description 1
- 244000070406 Malus silvestris Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 240000003433 Miscanthus floridulus Species 0.000 description 1
- 241000714177 Murine leukemia virus Species 0.000 description 1
- 240000005561 Musa balbisiana Species 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 101710202365 Napin Proteins 0.000 description 1
- 235000015742 Nephelium litchi Nutrition 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108091007494 Nucleic acid- binding domains Proteins 0.000 description 1
- 102000002488 Nucleoplasmin Human genes 0.000 description 1
- 241000202223 Oenococcus Species 0.000 description 1
- 241000219925 Oenothera Species 0.000 description 1
- 235000004496 Oenothera biennis Nutrition 0.000 description 1
- 240000007817 Olea europaea Species 0.000 description 1
- 101710089395 Oleosin Proteins 0.000 description 1
- 241000927544 Olsenella Species 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241001520808 Panicum virgatum Species 0.000 description 1
- 240000001090 Papaver somniferum Species 0.000 description 1
- 235000008753 Papaver somniferum Nutrition 0.000 description 1
- 240000004370 Pastinaca sativa Species 0.000 description 1
- 235000017769 Pastinaca sativa subsp sativa Nutrition 0.000 description 1
- 101710091688 Patatin Proteins 0.000 description 1
- 241000192001 Pediococcus Species 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 235000008673 Persea americana Nutrition 0.000 description 1
- 244000025272 Persea americana Species 0.000 description 1
- 244000062780 Petroselinum sativum Species 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 101710163504 Phaseolin Proteins 0.000 description 1
- 241000218657 Picea Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 240000003889 Piper guineense Species 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 235000001855 Portulaca oleracea Nutrition 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 244000141353 Prunus domestica Species 0.000 description 1
- 240000005809 Prunus persica Species 0.000 description 1
- 235000006029 Prunus persica var nucipersica Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 244000017714 Prunus persica var. nucipersica Species 0.000 description 1
- 235000014441 Prunus serotina Nutrition 0.000 description 1
- 244000294611 Punica granatum Species 0.000 description 1
- 235000014360 Punica granatum Nutrition 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- 101150090155 R gene Proteins 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 235000001537 Ribes X gardonianum Nutrition 0.000 description 1
- 235000001535 Ribes X utile Nutrition 0.000 description 1
- 235000002357 Ribes grossularia Nutrition 0.000 description 1
- 235000016919 Ribes petraeum Nutrition 0.000 description 1
- 244000281247 Ribes rubrum Species 0.000 description 1
- 235000002355 Ribes spicatum Nutrition 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 101710097247 Ribulose bisphosphate carboxylase large chain Proteins 0.000 description 1
- 101710104360 Ribulose bisphosphate carboxylase large chain, chromosomal Proteins 0.000 description 1
- 240000000528 Ricinus communis Species 0.000 description 1
- 235000004443 Ricinus communis Nutrition 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000109329 Rosa xanthina Species 0.000 description 1
- 241001412173 Rubus canescens Species 0.000 description 1
- 235000017848 Rubus fruticosus Nutrition 0.000 description 1
- 235000011034 Rubus glaucus Nutrition 0.000 description 1
- 235000009122 Rubus idaeus Nutrition 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 235000007238 Secale cereale Nutrition 0.000 description 1
- 108010016634 Seed Storage Proteins Proteins 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 244000044822 Simmondsia californica Species 0.000 description 1
- 235000004433 Simmondsia californica Nutrition 0.000 description 1
- 101100020617 Solanum lycopersicum LAT52 gene Proteins 0.000 description 1
- 101100083699 Solanum lycopersicum LAT59 gene Proteins 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 101710154134 Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic Proteins 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000194020 Streptococcus thermophilus Species 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 108700026226 TATA Box Proteins 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 244000152045 Themeda triandra Species 0.000 description 1
- 235000009470 Theobroma cacao Nutrition 0.000 description 1
- 244000299461 Theobroma cacao Species 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 240000006909 Tilia x europaea Species 0.000 description 1
- 244000294925 Tragopogon dubius Species 0.000 description 1
- 235000004478 Tragopogon dubius Nutrition 0.000 description 1
- 235000012363 Tragopogon porrifolius Nutrition 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 235000019714 Triticale Nutrition 0.000 description 1
- 101710162629 Trypsin inhibitor Proteins 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 241000722921 Tulipa gesneriana Species 0.000 description 1
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Natural products O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 1
- 235000003095 Vaccinium corymbosum Nutrition 0.000 description 1
- 240000001717 Vaccinium macrocarpon Species 0.000 description 1
- 235000017537 Vaccinium myrtillus Nutrition 0.000 description 1
- 244000078534 Vaccinium myrtillus Species 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 101710196023 Vicilin Proteins 0.000 description 1
- 235000012544 Viola sororia Nutrition 0.000 description 1
- 241001106476 Violaceae Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 241000202221 Weissella Species 0.000 description 1
- 240000001781 Xanthosoma sagittifolium Species 0.000 description 1
- 235000017957 Xanthosoma sagittifolium Nutrition 0.000 description 1
- 241000482268 Zea mays subsp. mays Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 101710159466 [Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial Proteins 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000003293 antisense oligodeoxyribonucleotide Substances 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 235000016520 artichoke thistle Nutrition 0.000 description 1
- 210000004507 artificial chromosome Anatomy 0.000 description 1
- 235000000183 arugula Nutrition 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 101150088806 atpA gene Proteins 0.000 description 1
- 235000021015 bananas Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 1
- 235000021014 blueberries Nutrition 0.000 description 1
- 210000004900 c-terminal fragment Anatomy 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 235000009120 camo Nutrition 0.000 description 1
- 229960000846 camphor Drugs 0.000 description 1
- 229930008380 camphor Natural products 0.000 description 1
- 101150004101 cbbL gene Proteins 0.000 description 1
- 230000036978 cell physiology Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000017803 cinnamon Nutrition 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000013601 cosmid vector Substances 0.000 description 1
- 235000021019 cranberries Nutrition 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 108010060641 flavanone synthetase Proteins 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 235000004611 garlic Nutrition 0.000 description 1
- 238000010363 gene targeting Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 235000004554 glutamine Nutrition 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 235000021384 green leafy vegetables Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000011487 hemp Substances 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 108010083942 mannopine synthase Proteins 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- ONCZDRURRATYFI-QTCHDTBASA-N methyl (2z)-2-methoxyimino-2-[2-[[(e)-1-[3-(trifluoromethyl)phenyl]ethylideneamino]oxymethyl]phenyl]acetate Chemical compound CO\N=C(/C(=O)OC)C1=CC=CC=C1CO\N=C(/C)C1=CC=CC(C(F)(F)F)=C1 ONCZDRURRATYFI-QTCHDTBASA-N 0.000 description 1
- 108091070501 miRNA Proteins 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 235000010460 mustard Nutrition 0.000 description 1
- 238000007481 next generation sequencing Methods 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 108060005597 nucleoplasmin Proteins 0.000 description 1
- 244000080466 oignon Species 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 235000011197 perejil Nutrition 0.000 description 1
- LWTDZKXXJRRKDG-UHFFFAOYSA-N phaseollin Natural products C1OC2=CC(O)=CC=C2C2C1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-UHFFFAOYSA-N 0.000 description 1
- NONJJLVGHLVQQM-JHXYUMNGSA-N phenethicillin Chemical compound N([C@@H]1C(N2[C@H](C(C)(C)S[C@@H]21)C(O)=O)=O)C(=O)C(C)OC1=CC=CC=C1 NONJJLVGHLVQQM-JHXYUMNGSA-N 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 150000004713 phosphodiesters Chemical group 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000037039 plant physiology Effects 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 235000021018 plums Nutrition 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 235000021039 pomes Nutrition 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000012743 protein tagging Effects 0.000 description 1
- 101150075980 psbA gene Proteins 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 102000005912 ran GTP Binding Protein Human genes 0.000 description 1
- 235000021013 raspberries Nutrition 0.000 description 1
- 238000009790 rate-determining step (RDS) Methods 0.000 description 1
- 101150074945 rbcL gene Proteins 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000013605 shuttle vector Substances 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 101150019416 trpA gene Proteins 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 108700026215 vpr Genes Proteins 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 241000228158 x Triticosecale Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/85—Fusion polypeptide containing an RNA binding domain
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962930836P | 2019-11-05 | 2019-11-05 | |
| PCT/US2020/059045 WO2021092130A1 (en) | 2019-11-05 | 2020-11-05 | Compositions and methods for rna-encoded dna-replacement of alleles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL292434A true IL292434A (en) | 2022-06-01 |
Family
ID=75687119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL292434A IL292434A (en) | 2019-11-05 | 2020-11-05 | Compositions and methods for rna-encoded dna-replacement of alleles |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US11926834B2 (https=) |
| EP (1) | EP4054621A4 (https=) |
| JP (1) | JP7785002B2 (https=) |
| KR (1) | KR20220110748A (https=) |
| CN (1) | CN114901302A (https=) |
| AU (1) | AU2020380319A1 (https=) |
| BR (1) | BR112022008415A2 (https=) |
| CA (1) | CA3160186A1 (https=) |
| CL (1) | CL2022001145A1 (https=) |
| IL (1) | IL292434A (https=) |
| MX (1) | MX2022005328A (https=) |
| NZ (1) | NZ787446A (https=) |
| WO (1) | WO2021092130A1 (https=) |
| ZA (1) | ZA202204756B (https=) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| AU2020369599A1 (en) | 2019-10-23 | 2022-05-12 | Monsanto Technology Llc | Compositions and methods for RNA-templated editing in plants |
| CA3160186A1 (en) | 2019-11-05 | 2021-05-14 | Pairwise Plants Services, Inc. | Compositions and methods for rna-encoded dna-replacement of alleles |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| US20220145334A1 (en) * | 2020-11-06 | 2022-05-12 | Pairwise Plants Services, Inc. | Compositions and methods for rna-encoded dna-replacement of alleles |
| EP4347818A2 (en) * | 2021-06-01 | 2024-04-10 | Arbor Biotechnologies, Inc. | Gene editing systems comprising a crispr nuclease and uses thereof |
| US20220403475A1 (en) | 2021-06-14 | 2022-12-22 | Pairwise Plants Services, Inc. | Reporter constructs, compositions comprising the same, and methods of use thereof |
| AU2022343268A1 (en) | 2021-09-08 | 2024-03-28 | Flagship Pioneering Innovations Vi, Llc | Methods and compositions for modulating a genome |
| WO2023049728A1 (en) | 2021-09-21 | 2023-03-30 | Pairwise Plants Services, Inc. | Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same |
| EP4596696A3 (en) | 2021-09-28 | 2025-08-27 | Acrigen Biosciences | Compositions and methods for nucleic acid modifications |
| WO2023102329A2 (en) * | 2021-11-30 | 2023-06-08 | Mammoth Biosciences, Inc. | Effector proteins and uses thereof |
| CN114317492A (zh) * | 2021-12-06 | 2022-04-12 | 北京大学 | 一种改造的人工核酸酶系统及其应用 |
| CN116064512A (zh) * | 2022-07-15 | 2023-05-05 | 山西大学 | 一种改进的引导编辑系统及其应用 |
| JP2025525569A (ja) * | 2022-07-18 | 2025-08-05 | レナゲード セラピューティクス マネージメント インコーポレイテッド | 遺伝子編集成分、システム、及び使用方法 |
| US12264328B2 (en) | 2022-07-18 | 2025-04-01 | ReNAgade Therapeutics Management, Inc. | Gene editing components, systems, and methods of use |
| CA3268411A1 (en) * | 2022-09-23 | 2024-03-28 | Flagship Pioneering Innovations Vi, Llc | METHODS FOR ENRICHING TARGET NUCLEIC ACID SEQUENCES |
| WO2024096790A1 (en) * | 2022-10-31 | 2024-05-10 | Soledits Ab | Modular crispr system |
| EP4627083A1 (en) * | 2022-11-30 | 2025-10-08 | Arbor Biotechnologies, Inc. | Reverse transcriptase-mediated genetic editing of transthyretin (ttr) and uses thereof |
| EP4630542A2 (en) * | 2022-12-09 | 2025-10-15 | Metagenomi, Inc. | Retrotransposon compositions and methods of use |
| EP4634375A2 (en) | 2022-12-16 | 2025-10-22 | Pairwise Plants Services, Inc. | Fusion proteins comprising a cas12a polypeptide and an intein and methods of use therof |
| US20240271109A1 (en) | 2022-12-21 | 2024-08-15 | Pairwise Plants Services, Inc. | Engineered proteins and methods of use thereof |
| AU2024239266A1 (en) | 2023-03-20 | 2025-09-25 | Pioneer Hi-Bred International, Inc. | Cas polypeptides with altered pam recognition |
| AU2024348958A1 (en) | 2023-09-26 | 2026-04-02 | Pairwise Plants Services, Inc. | Fusion proteins, compositions comprising the same, and methods of use thereof |
| AU2024357941A1 (en) | 2023-10-09 | 2026-04-02 | Pairwise Plants Services, Inc. | Circular permutants, compositions comprising the same, and methods of use thereof |
| WO2026019871A1 (en) | 2024-07-17 | 2026-01-22 | Pairwise Plants Services, Inc. | Engineered cytidine deaminases |
| US20260022361A1 (en) | 2024-07-17 | 2026-01-22 | Pairwise Plants Services, Inc. | Engineered proteins and methods of use thereof |
| US12297441B1 (en) | 2024-09-11 | 2025-05-13 | Pairwise Plants Services, Inc. | Methods and compositions for modifying endocarp structure in Rubus plants |
| WO2026085100A1 (en) | 2024-10-15 | 2026-04-23 | Pairwise Plants Services, Inc. | Methods for high density planting of rubus plants comprising a mutation in the terminal flower (tfl) gene |
Family Cites Families (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ221259A (en) | 1986-07-31 | 1990-05-28 | Calgene Inc | Seed specific transcriptional regulation |
| US6040504A (en) | 1987-11-18 | 2000-03-21 | Novartis Finance Corporation | Cotton promoter |
| ATE112314T1 (de) | 1988-05-17 | 1994-10-15 | Lubrizol Genetics Inc | Pflanzliches ubiquitinpromotorsystem. |
| US5641876A (en) | 1990-01-05 | 1997-06-24 | Cornell Research Foundation, Inc. | Rice actin gene and promoter |
| ATE225853T1 (de) | 1990-04-12 | 2002-10-15 | Syngenta Participations Ag | Gewebe-spezifische promotoren |
| US5459252A (en) | 1991-01-31 | 1995-10-17 | North Carolina State University | Root specific gene promoter |
| US5604121A (en) | 1991-08-27 | 1997-02-18 | Agricultural Genetics Company Limited | Proteins with insecticidal properties against homopteran insects and their use in plant protection |
| UA48104C2 (uk) | 1991-10-04 | 2002-08-15 | Новартіс Аг | Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника |
| KR20010041129A (ko) | 1998-02-20 | 2001-05-15 | 돈 리사 로얄 | 화분 특이적 프로모터 |
| US7166770B2 (en) | 2000-03-27 | 2007-01-23 | Syngenta Participations Ag | Cestrum yellow leaf curling virus promoters |
| AR044724A1 (es) | 2000-03-27 | 2005-10-05 | Syngenta Participations Ag | Promotores del virus de la rizadura amarilla del cestrum |
| WO2005037993A2 (en) | 2003-10-06 | 2005-04-28 | Syngenta Participations Ag | Promoters functional in plant plastids |
| KR100537955B1 (ko) | 2003-10-29 | 2005-12-20 | 학교법인고려중앙학원 | 꽃가루 특이적 유전자 발현 프로모터 |
| WO2010144775A1 (en) | 2009-06-11 | 2010-12-16 | Syngenta Participations Ag | A method for the transient expression of nucleic acids in plants |
| KR102047336B1 (ko) | 2012-02-01 | 2019-11-22 | 다우 아그로사이언시즈 엘엘씨 | 신규한 클래스의 글리포세이트 저항성 유전자 |
| AU2013335451C1 (en) * | 2012-10-23 | 2024-07-04 | Toolgen Incorporated | Composition for cleaving a target DNA comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof |
| WO2014186686A2 (en) * | 2013-05-17 | 2014-11-20 | Two Blades Foundation | Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases |
| CN120574876A (zh) | 2013-08-22 | 2025-09-02 | 纳幕尔杜邦公司 | 使用向导rna/cas内切核酸酶系统的植物基因组修饰及其使用方法 |
| AU2015234204A1 (en) | 2014-03-20 | 2016-10-06 | Universite Laval | CRISPR-based methods and products for increasing frataxin levels and uses thereof |
| US20170219596A1 (en) | 2014-07-14 | 2017-08-03 | The Regents Of The University Of California | A protein tagging system for in vivo single molecule imaging and control of gene transcription |
| KR102357893B1 (ko) | 2014-08-05 | 2022-02-04 | 맵퀘스트 에스아 | Pd-1 에 결합하는 면역학적 시약 |
| EP4464338A3 (en) * | 2014-11-07 | 2025-02-12 | Editas Medicine, Inc. | Systems for improving crispr/cas-mediated genome-editing |
| KR102888521B1 (ko) * | 2015-04-06 | 2025-11-19 | 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 | Crispr/cas-매개 유전자 조절을 위한 화학적으로 변형된 가이드 rna |
| US20160362667A1 (en) * | 2015-06-10 | 2016-12-15 | Caribou Biosciences, Inc. | CRISPR-Cas Compositions and Methods |
| US10648020B2 (en) | 2015-06-18 | 2020-05-12 | The Broad Institute, Inc. | CRISPR enzymes and systems |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| BR112018008134A2 (pt) | 2015-10-20 | 2018-11-06 | Pioneer Hi Bred Int | método para restaurar a função de um produto gênico não funcional no genoma de uma célula, método para editar uma sequência nucleotídica no genoma de uma célula, planta ou planta de progênie, método para editar uma sequência nucleotídica no genoma de uma célula sem o uso de um molde polinucleotídico de modificação e método para entregar um complexo de rna guia/endonuclease cas numa célula |
| CN108473981B (zh) | 2015-12-04 | 2022-04-12 | 卡里布生物科学公司 | 工程化靶向核酸的核酸 |
| WO2017151444A1 (en) | 2016-02-29 | 2017-09-08 | Agilent Technologies, Inc. | Methods and compositions for blocking off-target nucleic acids from cleavage by crispr proteins |
| AU2017257274B2 (en) | 2016-04-19 | 2023-07-13 | Massachusetts Institute Of Technology | Novel CRISPR enzymes and systems |
| EP3463484B1 (en) * | 2016-05-27 | 2026-01-07 | The Regents of the University of California | Methods and compositions for targeting rna polymerases and non-coding rna biogenesis to specific loci |
| EP3464594A1 (en) | 2016-06-01 | 2019-04-10 | Kws Saat Se | Hybrid nucleic acid sequences for genome engineering |
| CN109790527A (zh) | 2016-07-26 | 2019-05-21 | 通用医疗公司 | 普氏菌属和弗朗西斯氏菌属的CRISPR1(Cpf1)的变体 |
| ES2985812T3 (es) | 2016-09-09 | 2024-11-07 | The Board Of Trustees Of The Leland Stanfordjunior Univ | Edición genómica de precisión de alto rendimiento |
| CA3047416A1 (en) | 2017-01-20 | 2018-07-26 | The Regents Of The University Of California | Targeted gene activation in plants |
| US11591589B2 (en) | 2017-04-21 | 2023-02-28 | The General Hospital Corporation | Variants of Cpf1 (Cas12a) with altered PAM specificity |
| WO2018202199A1 (en) | 2017-05-05 | 2018-11-08 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | Methods for isolating cells without the use of transgenic marker sequences |
| JP2019016859A (ja) | 2017-07-04 | 2019-01-31 | オリンパス株式会社 | 観察装置および観察方法 |
| US20210054404A1 (en) | 2017-08-22 | 2021-02-25 | Napigen, Inc. | Organelle genome modification using polynucleotide guided endonuclease |
| EP3728589A4 (en) * | 2017-12-22 | 2021-11-03 | G+Flas Life Sciences | CHEMICAL GENOMIC ENGINEERING MOLECULES AND PROCESSES |
| US11268092B2 (en) | 2018-01-12 | 2022-03-08 | GenEdit, Inc. | Structure-engineered guide RNA |
| WO2020191233A1 (en) * | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| AU2020369599A1 (en) | 2019-10-23 | 2022-05-12 | Monsanto Technology Llc | Compositions and methods for RNA-templated editing in plants |
| CA3160186A1 (en) | 2019-11-05 | 2021-05-14 | Pairwise Plants Services, Inc. | Compositions and methods for rna-encoded dna-replacement of alleles |
| US20220145334A1 (en) | 2020-11-06 | 2022-05-12 | Pairwise Plants Services, Inc. | Compositions and methods for rna-encoded dna-replacement of alleles |
-
2020
- 2020-11-05 CA CA3160186A patent/CA3160186A1/en active Pending
- 2020-11-05 US US17/090,334 patent/US11926834B2/en active Active
- 2020-11-05 NZ NZ787446A patent/NZ787446A/en unknown
- 2020-11-05 EP EP20884787.1A patent/EP4054621A4/en active Pending
- 2020-11-05 WO PCT/US2020/059045 patent/WO2021092130A1/en not_active Ceased
- 2020-11-05 BR BR112022008415A patent/BR112022008415A2/pt unknown
- 2020-11-05 IL IL292434A patent/IL292434A/en unknown
- 2020-11-05 AU AU2020380319A patent/AU2020380319A1/en active Pending
- 2020-11-05 CN CN202080084407.0A patent/CN114901302A/zh active Pending
- 2020-11-05 JP JP2022538683A patent/JP7785002B2/ja active Active
- 2020-11-05 MX MX2022005328A patent/MX2022005328A/es unknown
- 2020-11-05 KR KR1020227018853A patent/KR20220110748A/ko active Pending
-
2022
- 2022-04-28 ZA ZA2022/04756A patent/ZA202204756B/en unknown
- 2022-05-03 CL CL2022001145A patent/CL2022001145A1/es unknown
-
2024
- 2024-01-26 US US18/423,374 patent/US20240167045A1/en active Pending
- 2024-01-26 US US18/423,378 patent/US20240158804A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2020380319A1 (en) | 2022-05-12 |
| CN114901302A (zh) | 2022-08-12 |
| JP7785002B2 (ja) | 2025-12-12 |
| JP2023505600A (ja) | 2023-02-09 |
| US20240167045A1 (en) | 2024-05-23 |
| EP4054621A1 (en) | 2022-09-14 |
| US20240158804A1 (en) | 2024-05-16 |
| WO2021092130A1 (en) | 2021-05-14 |
| US11926834B2 (en) | 2024-03-12 |
| EP4054621A4 (en) | 2023-12-27 |
| CL2022001145A1 (es) | 2023-02-03 |
| CA3160186A1 (en) | 2021-05-14 |
| ZA202204756B (en) | 2024-10-30 |
| NZ787446A (en) | 2026-02-27 |
| US20210130835A1 (en) | 2021-05-06 |
| BR112022008415A2 (pt) | 2022-09-06 |
| KR20220110748A (ko) | 2022-08-09 |
| MX2022005328A (es) | 2022-07-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20240158804A1 (en) | Compositions and methods for rna-encoded dna-replacement of alleles | |
| US12084671B2 (en) | Optimized CRISPR-Cas nucleases and base editors and methods of use thereof | |
| US12331330B2 (en) | Compositions and methods for RNA-templated editing in plants | |
| US20210130827A1 (en) | Type v crispr-cas base editors and methods of use thereof | |
| US20220145334A1 (en) | Compositions and methods for rna-encoded dna-replacement of alleles | |
| US12173335B2 (en) | Recruitment of DNA polymerase for templated editing | |
| US20250207154A1 (en) | Compositions and methods for rna-encoded dna-replacement of alleles | |
| US20260022361A1 (en) | Engineered proteins and methods of use thereof | |
| US20260022365A1 (en) | Engineered cytidine deaminases | |
| WO2026096573A1 (en) | Engineered proteins and methods of use thereof |