ZA200606202B - Compositions, methods and uses for a novel family of peptides - Google Patents
Compositions, methods and uses for a novel family of peptides Download PDFInfo
- Publication number
- ZA200606202B ZA200606202B ZA200606202A ZA200606202A ZA200606202B ZA 200606202 B ZA200606202 B ZA 200606202B ZA 200606202 A ZA200606202 A ZA 200606202A ZA 200606202 A ZA200606202 A ZA 200606202A ZA 200606202 B ZA200606202 B ZA 200606202B
- Authority
- ZA
- South Africa
- Prior art keywords
- peptide
- isolated
- feed
- terminus
- carboxy
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims description 149
- 102000004196 processed proteins & peptides Human genes 0.000 title claims description 38
- 238000000034 method Methods 0.000 title claims description 28
- 239000000203 mixture Substances 0.000 title claims description 23
- 108090000623 proteins and genes Proteins 0.000 claims description 43
- 230000002829 reductive effect Effects 0.000 claims description 37
- 235000018102 proteins Nutrition 0.000 claims description 36
- 102000004169 proteins and genes Human genes 0.000 claims description 36
- 210000004899 c-terminal region Anatomy 0.000 claims description 32
- 239000003674 animal food additive Substances 0.000 claims description 26
- 150000008574 D-amino acids Chemical class 0.000 claims description 25
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 24
- 241000287828 Gallus gallus Species 0.000 claims description 23
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 22
- 241001465754 Metazoa Species 0.000 claims description 21
- 150000007523 nucleic acids Chemical class 0.000 claims description 21
- 108020004707 nucleic acids Proteins 0.000 claims description 20
- 102000039446 nucleic acids Human genes 0.000 claims description 20
- 239000000654 additive Substances 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 claims description 14
- 230000000996 additive effect Effects 0.000 claims description 14
- 230000012010 growth Effects 0.000 claims description 14
- 235000019786 weight gain Nutrition 0.000 claims description 14
- 230000001580 bacterial effect Effects 0.000 claims description 12
- 239000004474 valine Substances 0.000 claims description 12
- 230000015572 biosynthetic process Effects 0.000 claims description 11
- 239000002253 acid Substances 0.000 claims description 10
- 244000144977 poultry Species 0.000 claims description 10
- 241000458359 Brevibacillus sp. Species 0.000 claims description 9
- 244000144972 livestock Species 0.000 claims description 9
- 241000271566 Aves Species 0.000 claims description 8
- 241000017712 Brevibacillus texasporus Species 0.000 claims description 8
- 241000238557 Decapoda Species 0.000 claims description 8
- 235000013339 cereals Nutrition 0.000 claims description 8
- 239000007858 starting material Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 241000251468 Actinopterygii Species 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 241000588724 Escherichia coli Species 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 230000037396 body weight Effects 0.000 claims description 6
- 229920001184 polypeptide Polymers 0.000 claims description 6
- 239000003910 polypeptide antibiotic agent Substances 0.000 claims description 6
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 5
- 239000011782 vitamin Substances 0.000 claims description 5
- 235000013343 vitamin Nutrition 0.000 claims description 5
- 229930003231 vitamin Natural products 0.000 claims description 5
- 229940088594 vitamin Drugs 0.000 claims description 5
- 102000044503 Antimicrobial Peptides Human genes 0.000 claims description 4
- 108700042778 Antimicrobial Peptides Proteins 0.000 claims description 4
- 241000270666 Testudines Species 0.000 claims description 4
- 150000007513 acids Chemical class 0.000 claims description 4
- 230000000845 anti-microbial effect Effects 0.000 claims description 4
- 239000004599 antimicrobial Substances 0.000 claims description 4
- 235000012054 meals Nutrition 0.000 claims description 4
- 125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 claims description 4
- 239000013598 vector Substances 0.000 claims description 4
- 241000283690 Bos taurus Species 0.000 claims description 3
- 241000283707 Capra Species 0.000 claims description 3
- 241000283073 Equus caballus Species 0.000 claims description 3
- 241000192125 Firmicutes Species 0.000 claims description 3
- 240000005979 Hordeum vulgare Species 0.000 claims description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 3
- 241001494479 Pecora Species 0.000 claims description 3
- 241000286209 Phasianidae Species 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- 235000019764 Soybean Meal Nutrition 0.000 claims description 3
- 241000282898 Sus scrofa Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 241000209140 Triticum Species 0.000 claims description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 3
- 235000019197 fats Nutrition 0.000 claims description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 235000013336 milk Nutrition 0.000 claims description 3
- 239000008267 milk Substances 0.000 claims description 3
- 210000004080 milk Anatomy 0.000 claims description 3
- 239000011707 mineral Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000004455 soybean meal Substances 0.000 claims description 3
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 3
- 241000272525 Anas platyrhynchos Species 0.000 claims description 2
- 241000205046 Archaeoglobus Species 0.000 claims description 2
- 241000555281 Brevibacillus Species 0.000 claims description 2
- 244000025254 Cannabis sativa Species 0.000 claims description 2
- 241000272201 Columbiformes Species 0.000 claims description 2
- 241000194033 Enterococcus Species 0.000 claims description 2
- 235000019733 Fish meal Nutrition 0.000 claims description 2
- 241000233866 Fungi Species 0.000 claims description 2
- 241000606790 Haemophilus Species 0.000 claims description 2
- 241000203353 Methanococcus Species 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 102000035195 Peptidases Human genes 0.000 claims description 2
- 241000589516 Pseudomonas Species 0.000 claims description 2
- 235000007238 Secale cereale Nutrition 0.000 claims description 2
- 244000082988 Secale cereale Species 0.000 claims description 2
- 108010073771 Soybean Proteins Proteins 0.000 claims description 2
- 235000019714 Triticale Nutrition 0.000 claims description 2
- 239000005862 Whey Substances 0.000 claims description 2
- 102000007544 Whey Proteins Human genes 0.000 claims description 2
- 108010046377 Whey Proteins Proteins 0.000 claims description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 2
- 229940036811 bone meal Drugs 0.000 claims description 2
- 239000002374 bone meal Substances 0.000 claims description 2
- 239000004467 fishmeal Substances 0.000 claims description 2
- 235000003599 food sweetener Nutrition 0.000 claims description 2
- 235000009973 maize Nutrition 0.000 claims description 2
- 235000013372 meat Nutrition 0.000 claims description 2
- 210000002381 plasma Anatomy 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 235000020183 skimmed milk Nutrition 0.000 claims description 2
- 235000012424 soybean oil Nutrition 0.000 claims description 2
- 239000003549 soybean oil Substances 0.000 claims description 2
- 235000019710 soybean protein Nutrition 0.000 claims description 2
- 239000003765 sweetening agent Substances 0.000 claims description 2
- 235000015099 wheat brans Nutrition 0.000 claims description 2
- 241000228158 x Triticosecale Species 0.000 claims description 2
- 239000005996 Blood meal Substances 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- ORTFAQDWJHRMNX-UHFFFAOYSA-N hydroxidooxidocarbon(.) Chemical group O[C]=O ORTFAQDWJHRMNX-UHFFFAOYSA-N 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 108010000785 non-ribosomal peptide synthase Proteins 0.000 description 127
- 150000001413 amino acids Chemical class 0.000 description 54
- GAOZTHIDHYLHMS-KEOBGNEYSA-N monensin A Chemical compound C([C@@](O1)(C)[C@H]2CC[C@@](O2)(CC)[C@H]2[C@H](C[C@@H](O2)[C@@H]2[C@H](C[C@@H](C)[C@](O)(CO)O2)C)C)C[C@@]21C[C@H](O)[C@@H](C)[C@@H]([C@@H](C)[C@@H](OC)[C@H](C)C(O)=O)O2 GAOZTHIDHYLHMS-KEOBGNEYSA-N 0.000 description 53
- 229930191564 Monensin Natural products 0.000 description 52
- GAOZTHIDHYLHMS-UHFFFAOYSA-N Monensin A Natural products O1C(CC)(C2C(CC(O2)C2C(CC(C)C(O)(CO)O2)C)C)CCC1C(O1)(C)CCC21CC(O)C(C)C(C(C)C(OC)C(C)C(O)=O)O2 GAOZTHIDHYLHMS-UHFFFAOYSA-N 0.000 description 52
- 229960005358 monensin Drugs 0.000 description 52
- 229940024606 amino acid Drugs 0.000 description 47
- 235000001014 amino acid Nutrition 0.000 description 47
- 108010054309 bacitracin methylenedisalicylic acid Proteins 0.000 description 31
- 108020004705 Codon Proteins 0.000 description 29
- 239000000758 substrate Substances 0.000 description 23
- 230000001419 dependent effect Effects 0.000 description 19
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 18
- 230000003115 biocidal effect Effects 0.000 description 18
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 17
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 17
- 230000036961 partial effect Effects 0.000 description 17
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 16
- 230000001186 cumulative effect Effects 0.000 description 16
- 238000011282 treatment Methods 0.000 description 16
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 14
- 230000027455 binding Effects 0.000 description 14
- 238000009739 binding Methods 0.000 description 14
- 208000021017 Weight Gain Diseases 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 13
- 239000000047 product Substances 0.000 description 13
- 238000003556 assay Methods 0.000 description 12
- 239000000470 constituent Substances 0.000 description 12
- 230000004584 weight gain Effects 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 238000012163 sequencing technique Methods 0.000 description 11
- 125000000539 amino acid group Chemical group 0.000 description 10
- 239000003242 anti bacterial agent Substances 0.000 description 10
- 229940088710 antibiotic agent Drugs 0.000 description 9
- 239000000499 gel Substances 0.000 description 9
- 235000013594 poultry meat Nutrition 0.000 description 9
- 239000000523 sample Substances 0.000 description 9
- 238000004885 tandem mass spectrometry Methods 0.000 description 9
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 8
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 8
- 108090000854 Oxidoreductases Proteins 0.000 description 8
- 102000004316 Oxidoreductases Human genes 0.000 description 8
- 238000004949 mass spectrometry Methods 0.000 description 8
- 230000004048 modification Effects 0.000 description 8
- 238000012986 modification Methods 0.000 description 8
- 108020004414 DNA Proteins 0.000 description 7
- 108700026244 Open Reading Frames Proteins 0.000 description 7
- 235000013330 chicken meat Nutrition 0.000 description 7
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 239000000377 silicon dioxide Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 239000004472 Lysine Substances 0.000 description 6
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 230000004913 activation Effects 0.000 description 6
- 238000001994 activation Methods 0.000 description 6
- 238000013459 approach Methods 0.000 description 6
- 235000005911 diet Nutrition 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000012071 phase Substances 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 5
- 230000001476 alcoholic effect Effects 0.000 description 5
- 239000006227 byproduct Substances 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 235000019688 fish Nutrition 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- 230000009467 reduction Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 241000894007 species Species 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 4
- 125000000729 N-terminal amino-acid group Chemical group 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 4
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 4
- 102000005488 Thioesterase Human genes 0.000 description 4
- 238000007792 addition Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000037213 diet Effects 0.000 description 4
- 239000003925 fat Substances 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 229930182817 methionine Natural products 0.000 description 4
- 235000006109 methionine Nutrition 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 229960003104 ornithine Drugs 0.000 description 4
- 238000004007 reversed phase HPLC Methods 0.000 description 4
- 238000002864 sequence alignment Methods 0.000 description 4
- 108020002982 thioesterase Proteins 0.000 description 4
- 108010006533 ATP-Binding Cassette Transporters Proteins 0.000 description 3
- 102000005416 ATP-Binding Cassette Transporters Human genes 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 101000644386 Brevibacillus parabrevis Phenylalanine racemase [ATP-hydrolyzing] Proteins 0.000 description 3
- 101000906861 Chondromyces crocatus ATP-dependent tyrosine adenylase Proteins 0.000 description 3
- 241000631130 Chrysophyllum argenteum Species 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 3
- 108010026389 Gramicidin Proteins 0.000 description 3
- 125000003440 L-leucyl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] 0.000 description 3
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 3
- 108010059712 Pronase Proteins 0.000 description 3
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 description 3
- 241000188156 Tamu Species 0.000 description 3
- 239000007997 Tricine buffer Substances 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- 230000034994 death Effects 0.000 description 3
- 231100000517 death Toxicity 0.000 description 3
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000003792 electrolyte Substances 0.000 description 3
- 238000006345 epimerization reaction Methods 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 3
- 238000010149 post-hoc-test Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 241000193417 Brevibacillus laterosporus Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 description 2
- 239000004470 DL Methionine Substances 0.000 description 2
- 235000005903 Dioscorea Nutrition 0.000 description 2
- 241000234273 Dioscorea Species 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- -1 FMOC amino acids Chemical class 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 125000002061 L-isoleucyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])[C@](C([H])([H])[H])([H])C(C([H])([H])[H])([H])[H] 0.000 description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 101150109417 NRPS gene Proteins 0.000 description 2
- 240000008881 Oenanthe javanica Species 0.000 description 2
- 108091007187 Reductases Proteins 0.000 description 2
- 241000282849 Ruminantia Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
- 239000004473 Threonine Substances 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 230000006154 adenylylation Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 150000001299 aldehydes Chemical class 0.000 description 2
- 150000001408 amides Chemical group 0.000 description 2
- 239000001506 calcium phosphate Substances 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000003610 charcoal Substances 0.000 description 2
- 239000002026 chloroform extract Substances 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000009833 condensation Methods 0.000 description 2
- 230000005494 condensation Effects 0.000 description 2
- 229960003067 cystine Drugs 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000001212 derivatisation Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 229940038472 dicalcium phosphate Drugs 0.000 description 2
- 229910000390 dicalcium phosphate Inorganic materials 0.000 description 2
- 230000000378 dietary effect Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 2
- 235000011180 diphosphates Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 239000000543 intermediate Substances 0.000 description 2
- 229960000310 isoleucine Drugs 0.000 description 2
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- FFEARJCKVFRZRR-UHFFFAOYSA-N methionine Chemical compound CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 238000002887 multiple sequence alignment Methods 0.000 description 2
- 235000021049 nutrient content Nutrition 0.000 description 2
- 229940054441 o-phthalaldehyde Drugs 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- ZWLUXSQADUDCSB-UHFFFAOYSA-N phthalaldehyde Chemical compound O=CC1=CC=CC=C1C=O ZWLUXSQADUDCSB-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 229960002898 threonine Drugs 0.000 description 2
- 150000003587 threonine derivatives Chemical class 0.000 description 2
- 230000001052 transient effect Effects 0.000 description 2
- ZWCXYZRRTRDGQE-LUPIJMBPSA-N valyl gramicidin a Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@H](NC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](NC=O)C(C)C)CC(C)C)C(=O)NCCO)=CNC2=C1 ZWCXYZRRTRDGQE-LUPIJMBPSA-N 0.000 description 2
- CCAIIPMIAFGKSI-DMTCNVIQSA-N (2s,3r)-3-hydroxy-2-(methylazaniumyl)butanoate Chemical compound CN[C@@H]([C@@H](C)O)C(O)=O CCAIIPMIAFGKSI-DMTCNVIQSA-N 0.000 description 1
- VACMTKGMNYPSRC-IHWYPQMZSA-N (Z)-2-amino-3-hydroxybut-2-enoic acid Chemical compound C\C(O)=C(\N)C(O)=O VACMTKGMNYPSRC-IHWYPQMZSA-N 0.000 description 1
- AHQFCPOIMVMDEZ-UNISNWAASA-N (e,2s,3r,4r)-3-hydroxy-4-methyl-2-(methylamino)oct-6-enoic acid Chemical compound CN[C@H](C(O)=O)[C@H](O)[C@H](C)C\C=C\C AHQFCPOIMVMDEZ-UNISNWAASA-N 0.000 description 1
- 125000003088 (fluoren-9-ylmethoxy)carbonyl group Chemical group 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
- 102100022749 Aminopeptidase N Human genes 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 206010003497 Asphyxia Diseases 0.000 description 1
- 241001061264 Astragalus Species 0.000 description 1
- 235000010110 Astragalus glycyphyllos Nutrition 0.000 description 1
- 241000132012 Atractylodes Species 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000304886 Bacilli Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 101100137463 Bacillus subtilis (strain 168) ppsA gene Proteins 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 241000589968 Borrelia Species 0.000 description 1
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 240000000385 Brassica napus var. napus Species 0.000 description 1
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 108010049990 CD13 Antigens Proteins 0.000 description 1
- 101100129088 Caenorhabditis elegans lys-2 gene Proteins 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 102000005572 Cathepsin A Human genes 0.000 description 1
- 108010059081 Cathepsin A Proteins 0.000 description 1
- 240000001817 Cereus hexagonus Species 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 241000675108 Citrus tangerina Species 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 241000252100 Conger Species 0.000 description 1
- 238000011537 Coomassie blue staining Methods 0.000 description 1
- 235000009917 Crataegus X brevipes Nutrition 0.000 description 1
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 description 1
- 235000009685 Crataegus X maligna Nutrition 0.000 description 1
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 description 1
- 235000009486 Crataegus bullatus Nutrition 0.000 description 1
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 description 1
- 235000009682 Crataegus limnophila Nutrition 0.000 description 1
- 235000004423 Crataegus monogyna Nutrition 0.000 description 1
- 240000000171 Crataegus monogyna Species 0.000 description 1
- 235000002313 Crataegus paludosa Nutrition 0.000 description 1
- 235000009840 Crataegus x incaedua Nutrition 0.000 description 1
- 101000979117 Curvularia clavata Nonribosomal peptide synthetase Proteins 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 125000003301 D-leucyl group Chemical group N[C@@H](C(=O)*)CC(C)C 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 101100045390 Drosophila melanogaster Tao gene Proteins 0.000 description 1
- 239000002000 Electrolyte additive Substances 0.000 description 1
- 241000194031 Enterococcus faecium Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 101000993347 Gallus gallus Ciliary neurotrophic factor Proteins 0.000 description 1
- 101000933252 Homo sapiens Protein BEX3 Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 241001597010 Kurtus gulliveri Species 0.000 description 1
- SRBFZHDQGSBBOR-HWQSCIPKSA-N L-arabinopyranose Chemical compound O[C@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-HWQSCIPKSA-N 0.000 description 1
- 102000004407 Lactalbumin Human genes 0.000 description 1
- 108090000942 Lactalbumin Proteins 0.000 description 1
- 108010028921 Lipopeptides Proteins 0.000 description 1
- 208000016604 Lyme disease Diseases 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 244000137850 Marrubium vulgare Species 0.000 description 1
- 235000006679 Mentha X verticillata Nutrition 0.000 description 1
- 235000002899 Mentha suaveolens Nutrition 0.000 description 1
- 235000001636 Mentha x rotundifolia Nutrition 0.000 description 1
- 241001024304 Mino Species 0.000 description 1
- 101100183772 Mus musculus Mfap2 gene Proteins 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- 229930183781 Mycobactin Natural products 0.000 description 1
- 102100030856 Myoglobin Human genes 0.000 description 1
- 108010062374 Myoglobin Proteins 0.000 description 1
- WNBVGSNESTZACV-UHFFFAOYSA-N Myxochelin A Natural products C=1C=CC(O)=C(O)C=1C(=O)NC(CO)CCCCNC(=O)C1=CC=CC(O)=C1O WNBVGSNESTZACV-UHFFFAOYSA-N 0.000 description 1
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 1
- 229920002274 Nalgene Polymers 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 241000272458 Numididae Species 0.000 description 1
- 241001327682 Oncorhynchus mykiss irideus Species 0.000 description 1
- 241001282110 Pagrus major Species 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- 108700018928 Peptide Synthases Proteins 0.000 description 1
- 206010071301 Perihepatitis Diseases 0.000 description 1
- 241000009328 Perro Species 0.000 description 1
- 241001495084 Phylo Species 0.000 description 1
- 241000269908 Platichthys flesus Species 0.000 description 1
- 241000861915 Plecoglossus Species 0.000 description 1
- 241001600434 Plectroglyphidodon lacrymatus Species 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 239000004721 Polyphenylene oxide Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 102100025955 Protein BEX3 Human genes 0.000 description 1
- 206010037075 Protozoal infections Diseases 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 101100342406 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PRS1 gene Proteins 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000277331 Salmonidae Species 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 241000223892 Tetrahymena Species 0.000 description 1
- 241001627955 Tetraodon lineatus Species 0.000 description 1
- 241000276707 Tilapia Species 0.000 description 1
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 1
- 102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 1
- 108700015934 Triose-phosphate isomerases Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- JAQGKXUEKGKTKX-HOTGVXAUSA-N Tyr-Tyr Chemical compound C([C@H](N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)C1=CC=C(O)C=C1 JAQGKXUEKGKTKX-HOTGVXAUSA-N 0.000 description 1
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 210000004712 air sac Anatomy 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 235000019730 animal feed additive Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 230000000842 anti-protozoal effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 239000003904 antiprotozoal agent Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 239000012300 argon atmosphere Substances 0.000 description 1
- 235000006533 astragalus Nutrition 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 101150074366 bhmt gene Proteins 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000001045 blue dye Substances 0.000 description 1
- 238000006664 bond formation reaction Methods 0.000 description 1
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 241001233037 catfish Species 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000003224 coccidiostatic agent Substances 0.000 description 1
- 229940000425 combination drug Drugs 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 239000003596 drug target Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- IUAYMJGZBVDSGL-XNNAEKOYSA-N gramicidin S Chemical compound C([C@@H]1C(=O)N2CCC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CCCN)C(=O)N[C@H](C(N[C@H](CC=2C=CC=CC=2)C(=O)N2CCC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CCCN)C(=O)N[C@@H](CC(C)C)C(=O)N1)C(C)C)=O)CC(C)C)C(C)C)C1=CC=CC=C1 IUAYMJGZBVDSGL-XNNAEKOYSA-N 0.000 description 1
- 229950009774 gramicidin s Drugs 0.000 description 1
- ZWCXYZRRTRDGQE-SORVKSEFSA-N gramicidina Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@H](NC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](NC=O)C(C)C)CC(C)C)C(=O)NCCO)=CNC2=C1 ZWCXYZRRTRDGQE-SORVKSEFSA-N 0.000 description 1
- 230000008642 heat stress Effects 0.000 description 1
- 230000007236 host immunity Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005567 liquid scintillation counting Methods 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- XZGYBQIQSLSHDH-COEJQBHMSA-N mycobactin Chemical compound C1CCCN(O)C(=O)C1NC(=O)C(C)C(CC)OC(=O)C(CCCCN(O)C(=O)\C=C/CCCCCCCCCCCCCCC)NC(=O)C(N=1)COC=1C1=C(C)C=CC=C1O XZGYBQIQSLSHDH-COEJQBHMSA-N 0.000 description 1
- WNBVGSNESTZACV-LBPRGKRZSA-N n-[(5s)-5-[(2,3-dihydroxybenzoyl)amino]-6-hydroxyhexyl]-2,3-dihydroxybenzamide Chemical compound C([C@@H](CO)NC(=O)C=1C(=C(O)C=CC=1)O)CCCNC(=O)C1=CC=CC(O)=C1O WNBVGSNESTZACV-LBPRGKRZSA-N 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000012074 organic phase Substances 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000004466 pelleted feed Substances 0.000 description 1
- CWEFIMQKSZFZNY-UHFFFAOYSA-N pentyl 2-[4-[[4-[4-[[4-[[4-(pentoxycarbonylamino)phenyl]methyl]phenyl]carbamoyloxy]butoxycarbonylamino]phenyl]methyl]phenyl]acetate Chemical compound C1=CC(CC(=O)OCCCCC)=CC=C1CC(C=C1)=CC=C1NC(=O)OCCCCOC(=O)NC(C=C1)=CC=C1CC1=CC=C(NC(=O)OCCCCC)C=C1 CWEFIMQKSZFZNY-UHFFFAOYSA-N 0.000 description 1
- 238000010647 peptide synthesis reaction Methods 0.000 description 1
- 208000008494 pericarditis Diseases 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 239000002460 polyether antibiotic agent Substances 0.000 description 1
- 229930001119 polyketide Natural products 0.000 description 1
- 229930001118 polyketide hybrid Natural products 0.000 description 1
- 125000003308 polyketide hybrid group Chemical group 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 101150056693 pps1 gene Proteins 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 239000004458 spent grain Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 238000011191 terminal modification Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
- 238000006177 thiolation reaction Methods 0.000 description 1
- 150000003588 threonines Chemical class 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 108010003137 tyrosyltyrosine Proteins 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 235000021241 α-lactalbumin Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/345—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Brevibacterium (G)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/10—Peptides having 12 to 20 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/164—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Epidemiology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Biotechnology (AREA)
- Animal Husbandry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Fodder In General (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US54056904P | 2004-01-30 | 2004-01-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200606202B true ZA200606202B (en) | 2008-04-30 |
Family
ID=34837403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200606202A ZA200606202B (en) | 2004-01-30 | 2006-07-26 | Compositions, methods and uses for a novel family of peptides |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US7498404B2 (no) |
| EP (1) | EP1765374A4 (no) |
| JP (1) | JP2008508851A (no) |
| KR (1) | KR20070033956A (no) |
| CN (1) | CN101217970A (no) |
| AU (1) | AU2005209901A1 (no) |
| BR (1) | BRPI0507186A (no) |
| CA (1) | CA2554634A1 (no) |
| EA (1) | EA200601408A1 (no) |
| IL (1) | IL177143A0 (no) |
| MX (1) | MXPA06008503A (no) |
| NO (1) | NO20063521L (no) |
| WO (1) | WO2005074626A2 (no) |
| ZA (1) | ZA200606202B (no) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8673290B2 (en) | 2011-03-01 | 2014-03-18 | MYGALAXY Limited Company | Sporulation-deficient B. texasporus cells and methods for efficient and cost-effective inactivation and use thereof |
| CN102835591B (zh) * | 2012-08-14 | 2017-09-15 | 北京大北农科技集团股份有限公司 | 一种易消化的哺乳仔猪教槽料及其制备方法 |
| JP6452607B2 (ja) | 2012-09-25 | 2019-01-16 | アキロン セイエレ エセ．エレ． | プロバイオティクス組成物及びプレバイオティクス組成物 |
| CN104780931B (zh) * | 2012-12-17 | 2018-05-18 | 星系有限公司 | Bt脂肽作为用于肥胖和相关疾病的治疗剂的用途 |
| WO2015187855A1 (en) | 2014-06-03 | 2015-12-10 | The Regents Of The University Of California | Non-invasive gene mutation detection in lung cancer patients |
| RU2603623C2 (ru) * | 2014-06-06 | 2016-11-27 | Олег Ильич Эпштейн | Ветеринарная композиция и способ улучшения жизнеспособности животных, стимуляции прироста живой массы млекопитающих и птиц, повышения эффективности иммунизации, профилактики и/или лечения инфекционных заболеваний (варианты) |
| ES2802453T3 (es) * | 2016-03-24 | 2021-01-19 | Allflex Europe Sa | Uso de una composición acuosa para la disolución de biomoléculas de una muestra de tejido |
| CN106597681A (zh) * | 2017-02-12 | 2017-04-26 | 李忠波 | 一种3d眼镜 |
| CN107347792A (zh) * | 2017-07-14 | 2017-11-17 | 叙永县元天生态养殖有限责任公司 | 一种乌骨鸡高山人工饲养方法 |
| CN110577910B (zh) * | 2019-09-17 | 2022-02-01 | 南京农业大学 | 一种侧孢短芽孢杆菌、抗菌脂肽及其在农业与食品上的应用 |
| CN111269865B (zh) * | 2020-04-01 | 2020-12-01 | 北京工商大学 | 一种侧孢短芽孢杆菌菌株s62-9及其用途 |
| CN111280182B (zh) * | 2020-04-01 | 2020-12-01 | 北京工商大学 | 一种活性肽组合物及其应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4504471A (en) * | 1982-09-27 | 1985-03-12 | Fujisawa Pharmaceutical Co., Ltd. | Animal growth promotant and method of use for animal growth |
| DE69617641T3 (de) * | 1995-01-26 | 2009-10-22 | Novozymes A/S | Xylanase beinhaltende futterzusätze für tiere |
| US5709894A (en) * | 1995-06-07 | 1998-01-20 | Biovance Nebraska | Feed additive for ruminant animals and a method for feeding a ruminant |
| DE19909146A1 (de) | 1999-03-03 | 2000-09-28 | Mohamed A Marahiel | Verfahren zur gezielten biologischen Synthese von Peptiden |
| CA2337923A1 (en) * | 2000-02-25 | 2001-08-25 | Raymond J. Andersen | Peptide antibiotics |
| JP2002058434A (ja) * | 2000-08-21 | 2002-02-26 | Nisshin Shiryo Kk | 動物用飼料添加物 |
| US6682762B2 (en) * | 2001-10-09 | 2004-01-27 | Heart-O-Dixie Animal Nutrition, Llc | Poultry and livestock feed additive |
| PT1448595E (pt) | 2001-11-20 | 2007-02-28 | Novozymes As | Polipéptideos antimicrobianos de pseudoplectania nigrella |
-
2005
- 2005-01-28 CN CNA2005800095123A patent/CN101217970A/zh active Pending
- 2005-01-28 BR BRPI0507186-0A patent/BRPI0507186A/pt not_active IP Right Cessation
- 2005-01-28 CA CA002554634A patent/CA2554634A1/en not_active Abandoned
- 2005-01-28 US US11/046,560 patent/US7498404B2/en not_active Expired - Fee Related
- 2005-01-28 MX MXPA06008503A patent/MXPA06008503A/es active IP Right Grant
- 2005-01-28 WO PCT/US2005/003343 patent/WO2005074626A2/en active Application Filing
- 2005-01-28 EP EP05722693A patent/EP1765374A4/en not_active Withdrawn
- 2005-01-28 AU AU2005209901A patent/AU2005209901A1/en not_active Abandoned
- 2005-01-28 KR KR1020067017586A patent/KR20070033956A/ko not_active Application Discontinuation
- 2005-01-28 EA EA200601408A patent/EA200601408A1/ru unknown
- 2005-01-28 JP JP2006551608A patent/JP2008508851A/ja active Pending
-
2006
- 2006-07-26 ZA ZA200606202A patent/ZA200606202B/en unknown
- 2006-07-27 IL IL177143A patent/IL177143A0/en unknown
- 2006-08-02 NO NO20063521A patent/NO20063521L/no not_active Application Discontinuation
-
2009
- 2009-03-02 US US12/396,221 patent/US20090175981A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005074626A2 (en) | 2005-08-18 |
| JP2008508851A (ja) | 2008-03-27 |
| EP1765374A4 (en) | 2010-11-03 |
| KR20070033956A (ko) | 2007-03-27 |
| AU2005209901A1 (en) | 2005-08-18 |
| US20060228398A1 (en) | 2006-10-12 |
| US20090175981A1 (en) | 2009-07-09 |
| WO2005074626A3 (en) | 2007-09-13 |
| US7498404B2 (en) | 2009-03-03 |
| IL177143A0 (en) | 2008-03-20 |
| EA200601408A1 (ru) | 2007-08-31 |
| BRPI0507186A (pt) | 2007-06-26 |
| EP1765374A2 (en) | 2007-03-28 |
| CN101217970A (zh) | 2008-07-09 |
| CA2554634A1 (en) | 2005-08-18 |
| MXPA06008503A (es) | 2007-01-30 |
| NO20063521L (no) | 2006-10-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7498404B2 (en) | Compositions, methods and uses for a novel family of peptides | |
| Wei et al. | Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides | |
| Rebuffat et al. | Microcin J25, from the macrocyclic to the lasso structure: implications for biosynthetic, evolutionary and biotechnological perspectives | |
| EP2379705B2 (en) | A bile resistant bacillus composition | |
| WO2016118850A1 (en) | Bacillus subtilis subspecies | |
| US20190231828A1 (en) | Methods of microbial treatment of poultry | |
| KR20230072517A (ko) | 프로바이오틱 바실루스 조성물 및 사용 방법 | |
| Mai et al. | cDNA cloning, expression and antibacterial activity of lysozyme C in the blue shrimp (Litopenaeus stylirostris) | |
| KR101892072B1 (ko) | 돌돔 유래의 신규한 피스시딘 펩티드 및 이의 용도 | |
| Ishii et al. | Dietary supplementation with lysine and threonine modulates the performance and plasma metabolites of broiler chicken | |
| Wu et al. | Structure and biosynthesis of the BT peptide antibiotic from Brevibacillus texasporus | |
| KR102004153B1 (ko) | 갯지렁이 유래의 신규한 더로마신 펩티드 및 이의 용도 | |
| CN108977455B (zh) | 用于生产草酸脱羧酶的重组质粒、大肠杆菌表达系统及方法和应用 | |
| IE59228B1 (en) | Kibdelosporangium aridum sk&f-aad-609 | |
| Jing et al. | An ancient molecule with novel function: Alanine aminotransferase as a lipopolysaccharide binding protein with bacteriocidal activity | |
| WO2023019198A2 (en) | Recombinant bacteria engineered to treat diseases associated with methionine metabolism and methods of use thereof | |
| KR101121077B1 (ko) | 넙치 유래의 항균성 펩타이드인 베타디펜신을 암호화하는 신규한 유전자 및 그의 용도 | |
| KR101508693B1 (ko) | 참굴 유래의 새로운 항미생물성 펩타이드 및 이의 용도 | |
| CN106967740B (zh) | 一种大肠杆菌融合表达菌丝霉素、其制备方法及应用 | |
| WO2001009171A1 (fr) | Nouveaux peptides de la tachykinine, leurs peptides precurseurs et genes les codant | |
| KR102569795B1 (ko) | 강도다리에서 유래한 유비퀴틴 관련 신규 항균 펩티드 및 이의 용도 | |
| KR102420699B1 (ko) | 강도다리 유래의 신규한 항균 펩티드 및 이의 용도 | |
| CN112760275B (zh) | 一种生产卟啉类化合物的重组菌及生产卟啉类化合物的方法 | |
| US20230181684A1 (en) | Novel antibiotic compositions and methods of making or using the same | |
| CN104761625A (zh) | 防御素mNP-1在促进鸡体重增加中的应用 |