WO2023279551A1 - Compound microbial fertilizer, preparation method therefor and application thereof - Google Patents
Compound microbial fertilizer, preparation method therefor and application thereof Download PDFInfo
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- WO2023279551A1 WO2023279551A1 PCT/CN2021/121649 CN2021121649W WO2023279551A1 WO 2023279551 A1 WO2023279551 A1 WO 2023279551A1 CN 2021121649 W CN2021121649 W CN 2021121649W WO 2023279551 A1 WO2023279551 A1 WO 2023279551A1
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- Prior art keywords
- microbial fertilizer
- compound microbial
- arthrobacter
- fermentation
- liquid
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 100
- 230000000813 microbial effect Effects 0.000 title claims abstract description 95
- 150000001875 compounds Chemical class 0.000 title claims abstract description 89
- 238000002360 preparation method Methods 0.000 title claims description 18
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 75
- 238000000855 fermentation Methods 0.000 claims abstract description 59
- 230000004151 fermentation Effects 0.000 claims abstract description 59
- 239000007788 liquid Substances 0.000 claims abstract description 47
- 239000002689 soil Substances 0.000 claims abstract description 45
- 241000186063 Arthrobacter Species 0.000 claims abstract description 32
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims abstract description 16
- 239000006012 monoammonium phosphate Substances 0.000 claims abstract description 16
- 235000019837 monoammonium phosphate Nutrition 0.000 claims abstract description 16
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000004202 carbamide Substances 0.000 claims abstract description 14
- 235000013877 carbamide Nutrition 0.000 claims abstract description 14
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims abstract description 14
- 229910052939 potassium sulfate Inorganic materials 0.000 claims abstract description 14
- 235000011151 potassium sulphates Nutrition 0.000 claims abstract description 14
- 235000011114 ammonium hydroxide Nutrition 0.000 claims abstract description 12
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims abstract description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 48
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 24
- 229910052757 nitrogen Inorganic materials 0.000 claims description 24
- 229910052698 phosphorus Inorganic materials 0.000 claims description 24
- 239000011574 phosphorus Substances 0.000 claims description 24
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 23
- 229910052700 potassium Inorganic materials 0.000 claims description 23
- 239000011591 potassium Substances 0.000 claims description 23
- 239000005416 organic matter Substances 0.000 claims description 22
- 239000011259 mixed solution Substances 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 11
- 239000003513 alkali Substances 0.000 claims description 7
- 238000005303 weighing Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 230000001737 promoting effect Effects 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 2
- 239000002131 composite material Substances 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 230000008635 plant growth Effects 0.000 claims description 2
- 239000012137 tryptone Substances 0.000 claims description 2
- 239000012138 yeast extract Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 19
- 230000012010 growth Effects 0.000 abstract description 12
- 230000006872 improvement Effects 0.000 abstract description 5
- 235000010633 broth Nutrition 0.000 abstract 1
- 229920000742 Cotton Polymers 0.000 description 33
- 239000002028 Biomass Substances 0.000 description 32
- 241000219146 Gossypium Species 0.000 description 31
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 30
- 240000003768 Solanum lycopersicum Species 0.000 description 30
- 230000004720 fertilization Effects 0.000 description 24
- 238000011282 treatment Methods 0.000 description 23
- 241000196324 Embryophyta Species 0.000 description 14
- 235000013399 edible fruits Nutrition 0.000 description 12
- 230000003247 decreasing effect Effects 0.000 description 7
- 238000003973 irrigation Methods 0.000 description 6
- 230000002262 irrigation Effects 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 4
- 241000727501 Arthrobacter halodurans Species 0.000 description 4
- 239000004021 humic acid Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- WYWFMUBFNXLFJK-UHFFFAOYSA-N [Mo].[Sb] Chemical compound [Mo].[Sb] WYWFMUBFNXLFJK-UHFFFAOYSA-N 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 240000002024 Gossypium herbaceum Species 0.000 description 1
- 235000004341 Gossypium herbaceum Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000013064 chemical raw material Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000005048 flame photometry Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009103 reabsorption Effects 0.000 description 1
- -1 salt ions Chemical class 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Definitions
- the invention relates to the technical field of microbial fertilizers, in particular to a compound microbial fertilizer and its preparation method and application.
- citric acid As an important chemical raw material and food additive, citric acid is widely used in food, medicine, chemical and other industries. A large amount of tail liquid is produced during the production of citric acid.
- the tail liquid mainly contains starch, protein, carbohydrates, various organic acids, ammonia nitrogen and fat, etc., with a concentration of 20,000-30,000 mg/L. It belongs to high-concentration organic wastewater, such as direct discharge. It will pollute the environment and destroy the soil.
- Compound microbial fertilizer is a fertilizer that combines the advantages of organic fertilizer, chemical fertilizer and microbial fertilizer.
- Compound microbial fertilizer not only provides NPK and medium and trace elements required for crop growth, but also provides organic matter and beneficial microbial active bacteria for crops.
- microorganisms reproduce in large numbers with the assistance of organic matter, inorganic nutrients, water, and temperature, reducing the living space of harmful microorganisms and increasing the number of beneficial microorganisms in the soil.
- Microorganisms produce a large amount of organic acids.
- the phosphorus and potassium elements deposited in the soil for many years are partially dissolved and released for reabsorption and utilization by crops. After long-term use, the soil will become more and more loose and fertile.
- the organic fertilizer in the compound microbial fertilizer is mainly fermented with livestock and poultry manure as the main raw material.
- the citric acid fermentation tail liquid contains rich carbon sources, nitrogen sources and inorganic salts, which can just meet the needs of crop growth.
- the citric acid fermentation tail liquid is used as the main raw material to prepare compound microbial fertilizer, it can not only effectively avoid the pollution of the waste liquid to the environment, but also utilize the waste to meet the needs of crop growth.
- the present invention provides a compound microbial fertilizer, which can increase crop yield, promote crop growth, and have an improvement effect on soil.
- a compound microbial fertilizer comprising citric acid fermentation tail liquid, Arthrobacter fermentation liquid, urea, monoammonium phosphate and potassium sulfate; and the proportion of each component: (400 ⁇ 600)mL:(400 ⁇ 600)mL:( 50-80) g: (50-80) g: (40-60) g; and the pH value of the compound microbial fertilizer is adjusted to 5.5-7.0 with ammonia water.
- the organic matter content in the citric acid fermentation tail liquid is 280-300g/L.
- the number of viable bacteria in the Arthrobacter fermentation broth is ⁇ 0.5 ⁇ 10 8 cfu/ml.
- the mass concentration of the ammonia water is 31-35%.
- the organic matter content in the citric acid fermentation tail liquid is 280.45g/L
- the humic acid content is 3.45g/L
- the total nitrogen content is 120.34g/L
- the total phosphorus content is 3.48g/L
- the total potassium content is 20.67g/L, rich in nutrients, which can provide good organic matter and inorganic salt ions for the growth and development of crops
- Arthrobacter halodurans KJ-1 the preservation number is CCTCC NO: M 2021332, and the preservation time is 2021 On April 6, the depository unit was the China Type Culture Collection Center, and the depository address was China.Wuhan.Wuhan University; the classification was named Arthrobacter halodurans.
- Arthrobacter fermentation broth had more viable bacteria, and Arthrobacter could loosen the soil and promote the degradation of salt ions and heavy metal ions in the soil; urea, monoammonium phosphate and potassium sulfate could provide nitrogen, phosphorus and carbon sources for the soil. Therefore, in the composite microbial fertilizer of the present invention, the waste liquid in the citric acid fermentation process is used as the main raw material, which avoids environmental pollution. The mixing of the tail liquid of the fermentation liquid can significantly promote the growth and development of crops, and has the effect of improving soil.
- a preparation method for compound microbial fertilizer comprising the steps of:
- Adjusting the pH value adding ammonia water to the mixed solution to adjust the pH value to 6.5 to obtain a compound microbial fertilizer.
- the fermentation medium includes yeast extract, tryptone and distilled water, and the mass-volume ratio of the three is: 1g:2g:200ml.
- the pH value of the fermentation medium is 7.5-7.8.
- the pH value of the prepared compound microbial fertilizer is 5.5-7.0; the contents of nitrogen, phosphorus and potassium in the compound microbial fertilizer are 2.73%, 6.17% and 3.05% respectively.
- the compound microbial fertilizer provided by the present invention can promote the growth and output of tomatoes in non-saline-alkali land. Applying the compound microbial fertilizer of the present invention can promote the fresh biomass and dry biomass of tomatoes, which are respectively increased by 18.78% and 22.69% compared with conventional fertilization, and the single fruit weight is increased by 18.90% %, the output increased by 13.11%.
- the application of the compound microbial fertilizer of the present invention can reduce the pH value of the soil and increase the content of available potassium, available phosphorus, organic matter, and alkaline nitrogen by 8.13%, 18.01%, 17.64%, and 16.97%, respectively.
- the compound microbial fertilizer provided by the invention can promote the growth and yield of cotton in saline-alkali land, and the application of the compound microbial fertilizer of the present invention can promote the fresh biomass and dry biomass of cotton, which are respectively increased by 19.92% and 24.25% compared with conventional fertilization.
- the number of cotton bolls per plant increased by 14.74%, the weight of single bolls increased by 12.38%, and the yield of seed cotton significantly increased by 41.43% compared with the conventional fertilization treatment.
- the Arthrobacter halodurans KJ-1 used in the examples the preservation number is CCTCC NO: M 2021332, the preservation time is April 6, 2021, the preservation unit is the China Type Culture Collection Center, and the preservation address is Wuhan, China .Wuhan University; Classified as Arthrobacter halodurans.
- Embodiment 1 prepares compound microbial fertilizer
- a preparation method of compound microbial fertilizer comprising:
- a preparation method of compound microbial fertilizer comprising:
- a preparation method of compound microbial fertilizer comprising:
- CK is conventional field fertilization
- processing 1 is the application of the compound microbial fertilizer of embodiment 1 of the present invention
- processing 2 is the application of the compound microbial fertilizer of embodiment 2 of the present invention
- processing 3 For using the compound microbial fertilizer of embodiment 3 of the present invention, small treatment 4 is the compound microbial fertilizer on the market. 100kg/mu compound microbial fertilizer was applied at tomato seedling stage, full flower stage and full fruit stage respectively, and 100kg/mu of monoammonium phosphate was applied to CK. Then the tomato biomass, yield and soil basic nutrients of different treatments were detected. The test was carried out in Shizong Field, Shihezi City, Xinjiang.
- the test crop was tomato, and the test variety was 1612.
- the planting method was film-covered drip irrigation, 1 film 2 tubes 2 Row configuration, plant spacing 40cm, row spacing 60cm, theoretical plant number 5.2*104 plants ⁇ hm -2 .
- the total irrigation volume is 150m 3 mu, 8 times of irrigation, and 6 times of fertilization with water.
- Example 1 the application of the compound microbial fertilizer of Example 1 can promote the fresh biomass and dry biomass of tomato, which are respectively increased by 5.24% and 8.66% compared with conventional fertilization.
- Applying the compound microbial fertilizer of Example 2 can promote the fresh biomass and dry biomass of tomato, which are respectively increased by 18.78% and 22.69% compared with conventional fertilization.
- Applying the compound microbial fertilizer of Example 3 can promote the fresh biomass and dry biomass of tomato, which are respectively increased by 8.34% and 15.26% compared with conventional fertilization.
- the compound microbial fertilizers in the market can promote the fresh biomass and dry biomass of tomato, which are increased by 16.24% and 12.51% respectively compared with conventional fertilization.
- the above results show that applying the compound microbial fertilizer of Example 2 is the best for promoting tomato fresh biomass and dry biomass.
- Tomato yield per mu (number of fruit per plant * weight of single fruit * number of plants per mu * correlation coefficient)/1000. The results are shown in Table 2;
- the application of the compound microbial fertilizer of Example 1 has a significant promotion effect on the tomato yield, and the single fruit weight increased by 2.66% and the yield increased by 4.33% compared with the unapplied treatment.
- the application of the compound microbial fertilizer of Example 2 has a significant effect on promoting the yield of tomato, and the single fruit weight increased by 18.90% and the yield increased by 13.11% compared with the unapplied treatment.
- the application of the compound microbial fertilizer of Example 3 has a significant promotion effect on the tomato yield, and the single fruit weight increased by 15.18% and the yield increased by 4.98% compared with the unapplied treatment.
- the application of the compound microbial fertilizer of Example 3 has a significant promotion effect on the tomato yield, and the single fruit weight increased by 15.18% and the yield increased by 4.98% compared with the unapplied treatment.
- the application of compound microbial fertilizers in the market can significantly promote the yield of tomato, which increases the single fruit weight by 17.41% and the yield by 4.71% compared with the non-application treatment.
- the above results show that applying the compound microbial fertilizer of Example 2 improves the single fruit weight and yield of tomato to the maximum.
- the soil samples not applied with the compound microbial fertilizer of the present invention and applied with the compound microbial fertilizer of the present invention were collected at the tomato seedling pulling stage, passed through 1 mm and 0.15 mm sieves successively after air-drying.
- the soil was successively tested for organic matter (potassium dichromate volumetric method), pH value, alkaline hydrolysis nitrogen (by alkaline hydrolysis diffusion method), available phosphorus (by molybdenum antimony anti-colorimetric method), available potassium (by flame Photometer method), the results are shown in Table 3;
- Example 3 It can be seen from Table 3 that the application of the compound microbial fertilizer of Example 1 can reduce the pH value of the soil by 1.12%, and can increase the contents of available potassium, available phosphorus, organic matter and alkaline nitrogen by 2.46% and 4.02% respectively , 13.65%, 5.61%.
- Application of Example 2 compound microbial fertilizer can reduce the pH value of the soil, the reduction is 1.52%, and can increase the content of available potassium, available phosphorus, organic matter, and alkaline nitrogen, and the increase is respectively 8.13%, 18.01%, 17.64%, 16.97% %.
- Example 3 compound microbial fertilizer can reduce the pH value of the soil, the reduction is 0.25%, and can increase the content of available potassium, available phosphorus, organic matter, and alkaline nitrogen, and the increase is respectively 5.80%, 13.17%, 7.58%, 5.80% %.
- the application of commercially available compound microbial fertilizers can reduce the pH value of the soil by 0.10%, and increase the content of available potassium, available phosphorus, organic matter, and alkaline nitrogen by 4.84%, 14.91%, 6.87%, and 10.68%, respectively.
- the available nutrients of the soil can be improved after applying the compound microbial fertilizer, which has the effects of fertilizing soil fertility and improving soil quality, and the effect of applying the compound microbial fertilizer of Example 2 is the best.
- plots were set up for comparative experiments.
- the basic physical and chemical properties of the plots are pH: 8.91, EC: 3.41ms/cm, organic matter: 13.28g/kg, alkaline nitrogen: 13.92mg/kg, available phosphorus: 20.92mg/kg , Available potassium: 110.92mg/kg.
- CK is conventional field fertilization
- Process 1 is to use the compound microbial fertilizer of embodiment 1 of the present invention
- Process 2 is to use the compound microbial fertilizer of embodiment 2 of the present invention
- process 3 is to use the implementation of the present invention
- the compound microbial fertilizer of example 3 processing 4 is the compound microbial fertilizer on the market. 100kg/mu compound microbial fertilizer was applied at tomato seedling stage, full flower stage and full fruit stage respectively, and 100kg/mu of monoammonium phosphate was applied to CK.
- Cotton is planted with plastic film, mechanical on-demand, 1 film, 3 tubes and 6 rows, with a total irrigation volume of 350m 3 /mu, 8 times of irrigation, and 7 times of fertilization with water.
- Example 4 It can be seen from Table 4 that the application of the compound microbial fertilizer of Example 1 can promote the fresh biomass and dry biomass of cotton, which are respectively increased by 3.87% and 8.93% compared with conventional fertilization. Applying the compound microbial fertilizer of Example 2 can promote the fresh biomass and dry biomass of cotton, which are respectively increased by 19.92% and 24.25% compared with conventional fertilization. Applying the compound microbial fertilizer of Example 3 can promote the fresh biomass and dry biomass of cotton, which are respectively increased by 15.91% and 19.66% compared with conventional fertilization. The application of commercially available compound microbial fertilizers can increase the fresh biomass and dry biomass of cotton by 17.70% and 18.46%, respectively, compared with conventional fertilization. The above results show that the effect of using the compound microbial fertilizer of Example 2 is the best.
- the number of cotton bolls per plant increased by 10.37%, the weight of single bolls increased by 8.72%, and the yield of seed cotton significantly increased by 35.48% under the application of commercial compound microbial fertilizers compared with conventional fertilization treatments. It shows that the application of compound microbial fertilizer can increase the number of bolls per plant of cotton and the weight of single boll, thereby increasing the yield of seed cotton. Wherein, the number of bolls per plant of flowers, the weight of single boll and the yield of seed cotton increased by the application of compound microbial fertilizer in Example 2 are the largest.
- Example 6 As can be seen from Table 6, the application of the compound microbial fertilizer in Example 1 reduces the soil pH by 3.93% compared with the conventional fertilization treatment, EC reduces by 16.13%, organic matter increases by 8.13%, alkaline hydrolysis nitrogen increases by 17.89%, available phosphorus significantly increases by 12.62%, and available Potassium boosted by 9.95%.
- Application Example 2 compound microbial fertilizer treatment compared with conventional fertilization treatment soil pH decreased 7.09%, EC decreased 25.83%, organic matter increased 52.03%, alkaline hydrolysis nitrogen increased 42.31%, available phosphorus significantly increased 46.46%, available potassium increased 32.45%.
- compound microbial fertilizer can improve the available nutrients of saline-alkali soil and reduce pH, EC, and has the effect of fertilizing soil fertility and improving soil quality.
- the compound microbial fertilizer of Example 2 has the best improvement effect on saline-alkali soil.
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
Abstract
Disclosed is a compound microbial fertilizer, comprising a citric acid fermentation tail liquid, an arthrobacter fermentation broth, urea, monoammonium phosphate, and potassium sulfate in the ratio of (400-600) mL:(400-600) mL:(50-80) g:(50-80) g:(40-60) g. The pH value of the compound microbial fertilizer is adjusted to 5.5-7.0 by using ammonia water. The fertilizer can increase the crop yield and promote crop growth, and has an improvement effect on soil.
Description
本发明涉及微生物肥料技术领域，更具体地说是涉及一种复合微生物肥料及其制备方法与应用。The invention relates to the technical field of microbial fertilizers, in particular to a compound microbial fertilizer and its preparation method and application.
柠檬酸作为一种重要的化工原料和食品添加剂被广泛应用于食品、医药、化工等行业。柠檬酸生产过程中产生大量尾液，尾液中主要含有淀粉、蛋白质、碳水化合物、各种有机酸、氨氮和脂肪等，浓度为20000～30000mg/L，属于高浓度有机废水，如直接排放，会污染环境、破坏土壤。As an important chemical raw material and food additive, citric acid is widely used in food, medicine, chemical and other industries. A large amount of tail liquid is produced during the production of citric acid. The tail liquid mainly contains starch, protein, carbohydrates, various organic acids, ammonia nitrogen and fat, etc., with a concentration of 20,000-30,000 mg/L. It belongs to high-concentration organic wastewater, such as direct discharge. It will pollute the environment and destroy the soil.
复合微生物肥料是集有机肥、化学肥料和微生物肥料的优点于一体的肥料。复合微生物肥料不仅给作物生长提供所需的NPK和中微量元素外，还能为作物提供有机质和有益微生物活性菌。肥料进入土壤后，微生物在有机质、无机营养元素、水分、温度的协助下大量繁殖，减少了有害微生物群体的生存空间，从而增加了土壤有益微生物菌的数量，微生物菌产生大量的有机酸可以把多年沉积在土壤中的磷钾元素部分溶解释放出来供作物再次吸收利用，长期使用后土壤将会变得越来越疏松和肥沃。Compound microbial fertilizer is a fertilizer that combines the advantages of organic fertilizer, chemical fertilizer and microbial fertilizer. Compound microbial fertilizer not only provides NPK and medium and trace elements required for crop growth, but also provides organic matter and beneficial microbial active bacteria for crops. After the fertilizer enters the soil, microorganisms reproduce in large numbers with the assistance of organic matter, inorganic nutrients, water, and temperature, reducing the living space of harmful microorganisms and increasing the number of beneficial microorganisms in the soil. Microorganisms produce a large amount of organic acids. The phosphorus and potassium elements deposited in the soil for many years are partially dissolved and released for reabsorption and utilization by crops. After long-term use, the soil will become more and more loose and fertile.
目前，复合微生物肥料中的有机肥主要以畜禽粪便为主要原料进行发酵，然而，柠檬酸发酵尾液中含有丰富的碳源、氮源和无机盐等多种成分恰恰能满足作物生长所需，若以柠檬酸发酵尾液为主要原料制备复合微生物肥料，既能有效避免废液对环境的污染，又能废物利用，满足作物生长所需。At present, the organic fertilizer in the compound microbial fertilizer is mainly fermented with livestock and poultry manure as the main raw material. However, the citric acid fermentation tail liquid contains rich carbon sources, nitrogen sources and inorganic salts, which can just meet the needs of crop growth. , if the citric acid fermentation tail liquid is used as the main raw material to prepare compound microbial fertilizer, it can not only effectively avoid the pollution of the waste liquid to the environment, but also utilize the waste to meet the needs of crop growth.
因此，如何以柠檬酸发酵尾液为主要原料制备微生物肥料并将其施入土壤中促进作物生长是本领域技术人员亟需解决的问题。Therefore, how to use the citric acid fermentation tail liquid as the main raw material to prepare microbial fertilizer and apply it to the soil to promote crop growth is a problem that those skilled in the art need to solve urgently.
发明内容Contents of the invention
有鉴于此，本发明提供了一种复合微生物肥料，该肥料可提高作物产量，促进作物生长，且对土壤具有改良作用。In view of this, the present invention provides a compound microbial fertilizer, which can increase crop yield, promote crop growth, and have an improvement effect on soil.
为了实现上述目的，本发明采用如下技术方案：In order to achieve the above object, the present invention adopts the following technical solutions:
一种复合微生物肥料，包括柠檬酸发酵尾液、节杆菌发酵液、尿素、磷酸一铵和硫酸钾；且各组分的配比:(400～600)mL:(400～600)mL:(50～80)g:(50～80)g:(40～60)g；且所述复合微生物肥料以氨水调节pH值至5.5-7.0。A compound microbial fertilizer, comprising citric acid fermentation tail liquid, Arthrobacter fermentation liquid, urea, monoammonium phosphate and potassium sulfate; and the proportion of each component: (400～600)mL:(400～600)mL:( 50-80) g: (50-80) g: (40-60) g; and the pH value of the compound microbial fertilizer is adjusted to 5.5-7.0 with ammonia water.
作为本发明优选的技术方案，所述柠檬酸发酵尾液中有机质含量为280-300g/L。As a preferred technical solution of the present invention, the organic matter content in the citric acid fermentation tail liquid is 280-300g/L.
作为本发明优选的技术方案，所述节杆菌发酵液中的活菌数≥0.5×10
8cfu/ml。
As a preferred technical solution of the present invention, the number of viable bacteria in the Arthrobacter fermentation broth is ≥0.5×10 8 cfu/ml.
作为本发明优选的技术方案，所述氨水的质量浓度为31-35％。As a preferred technical solution of the present invention, the mass concentration of the ammonia water is 31-35%.
以上技术方案达到的技术效果是：柠檬酸发酵尾液中有机质含量为280.45g/L，腐殖酸含量为3.45g/L，总氮含量为120.34g/L，总磷含量为3.48g/L、总钾含量为20.67g/L，营养丰富，可为作物生长发育提供良好的有机质和无机盐离子；节杆菌为Arthrobacter halodurans KJ-1，保藏编号为CCTCC NO：M 2021332，保藏时间为2021年4月6日，保藏单位为中国典型培养物保藏中心，保藏地址为中国.武汉.武汉大学；分类命名为Arthrobacter halodurans。节杆菌发酵液中活菌数较多，节杆菌可疏松土壤，且促进土壤中盐离子和重金属离子的降解；尿素、磷酸一铵和硫酸钾可为土壤提供氮源、磷源和碳源。所以，本发明的复合微生物肥料，柠檬酸发酵过程中的废液为主要原料，避免了对环境的污染，同时，由于节杆菌发酵液具有耐盐耐旱的特性，将菌株发酵液与柠檬酸发酵液尾液混合，可显著促进作物的生长发育，且，具有改良土壤的作用。The technical effect achieved by the above technical scheme is: the organic matter content in the citric acid fermentation tail liquid is 280.45g/L, the humic acid content is 3.45g/L, the total nitrogen content is 120.34g/L, and the total phosphorus content is 3.48g/L , The total potassium content is 20.67g/L, rich in nutrients, which can provide good organic matter and inorganic salt ions for the growth and development of crops; Arthrobacter halodurans KJ-1, the preservation number is CCTCC NO: M 2021332, and the preservation time is 2021 On April 6, the depository unit was the China Type Culture Collection Center, and the depository address was China.Wuhan.Wuhan University; the classification was named Arthrobacter halodurans. Arthrobacter fermentation broth had more viable bacteria, and Arthrobacter could loosen the soil and promote the degradation of salt ions and heavy metal ions in the soil; urea, monoammonium phosphate and potassium sulfate could provide nitrogen, phosphorus and carbon sources for the soil. Therefore, in the composite microbial fertilizer of the present invention, the waste liquid in the citric acid fermentation process is used as the main raw material, which avoids environmental pollution. The mixing of the tail liquid of the fermentation liquid can significantly promote the growth and development of crops, and has the effect of improving soil.
一种复合微生物肥料的制备方法，包括下述步骤：A preparation method for compound microbial fertilizer, comprising the steps of:
1)制备节杆菌发酵液：将活化节杆菌菌株接种于发酵培养基中，37℃，180r/min摇床中发酵96h，得节杆菌发酵液；1) Preparation of Arthrobacter fermentation broth: Inoculate the activated Arthrobacter strain into the fermentation medium, ferment in a shaker at 37°C and 180r/min for 96h, and obtain the Arthrobacter fermentation broth;
2)称取：按照体积质量比称取柠檬酸发酵尾液、节杆菌发酵液、氨水、尿素、磷酸一铵和硫酸钾；2) Weighing: Weighing citric acid fermentation tail liquid, Arthrobacter fermentation liquid, ammonia water, urea, monoammonium phosphate and potassium sulfate according to volume to mass ratio;
3)初次混合：向柠檬酸发酵尾液中加入所述节杆菌发酵液，得发酵混合液；3) initial mixing: adding the Arthrobacter fermented liquid to the citric acid fermentation tail liquid to obtain a fermented mixed liquid;
4)二次混合：向所述发酵混合液中加入尿素、磷酸一铵、硫酸钾，搅拌至均一溶液，得混合液；4) Secondary mixing: adding urea, monoammonium phosphate, and potassium sulfate to the fermentation mixed solution, and stirring until a uniform solution was obtained to obtain a mixed solution;
5)调节pH值：向所述混合液中加入氨水，调节pH值至6.5，得复合微生物肥料。5) Adjusting the pH value: adding ammonia water to the mixed solution to adjust the pH value to 6.5 to obtain a compound microbial fertilizer.
作为本发明优选的技术方案，所述发酵培养基包括酵母提取物、胰蛋白胨和蒸馏水，且，三者的质量体积比为：1g:2g：200ml。As a preferred technical solution of the present invention, the fermentation medium includes yeast extract, tryptone and distilled water, and the mass-volume ratio of the three is: 1g:2g:200ml.
作为本发明优选的技术方案，所述发酵培养基的pH值为7.5-7.8。As a preferred technical solution of the present invention, the pH value of the fermentation medium is 7.5-7.8.
作为本发明优选的技术方案，制备得到的复合微生物肥料的pH值为5.5-7.0；复合微生物肥料中氮、磷、钾含量分别为2.73％、6.17％、3.05％。As a preferred technical solution of the present invention, the pH value of the prepared compound microbial fertilizer is 5.5-7.0; the contents of nitrogen, phosphorus and potassium in the compound microbial fertilizer are 2.73%, 6.17% and 3.05% respectively.
上述制备方法制备得到的复合微生物肥料在提高作物产量、促进植物生长中的应用。The application of the compound microbial fertilizer prepared by the above preparation method in improving crop yield and promoting plant growth.
上述制备方法制备得到的复合微生物肥料在改良盐碱地土壤中的应用。Application of the compound microbial fertilizer prepared by the above preparation method in improving saline-alkali soil.
综上所述，本发明的技术效果是：In sum, technical effect of the present invention is:
本发明提供的复合微生物肥料对非盐碱地番茄生长、产量有促进作用，施用本发明复合微生物肥料可促进番茄鲜生物量、干生物量，较常规施肥分别增加18.78％，22.69％，单果重增加18.90％，产量增加13.11％。此外，施用本发明复合微生物肥料可以降低土壤pH值，增加速效钾、速效磷、有机质、碱解氮含量，增加量分别为8.13％、18.01％、17.64％、16.97％。The compound microbial fertilizer provided by the present invention can promote the growth and output of tomatoes in non-saline-alkali land. Applying the compound microbial fertilizer of the present invention can promote the fresh biomass and dry biomass of tomatoes, which are respectively increased by 18.78% and 22.69% compared with conventional fertilization, and the single fruit weight is increased by 18.90% %, the output increased by 13.11%. In addition, the application of the compound microbial fertilizer of the present invention can reduce the pH value of the soil and increase the content of available potassium, available phosphorus, organic matter, and alkaline nitrogen by 8.13%, 18.01%, 17.64%, and 16.97%, respectively.
而且，本发明提供的复合微生物肥料对盐碱地棉花生长、产量有促进作用，施用本发明复合微生物肥料可促进棉花鲜生物量、干生物量，较常规施肥分别增加19.92％，24.25％。施用复合微生物肥料处理较常规施肥处理棉花单株铃数增加14.74％，单铃重增加12.38％，籽棉产量显著增加41.43％。此外，施用复合微生物肥料处理较常规施肥处理土壤pH降低7.09％，EC降低25.83％，有机质提高52.03％、碱解氮提高42.31％、速效磷显著提高46.46％、速效钾提高32.45％。Moreover, the compound microbial fertilizer provided by the invention can promote the growth and yield of cotton in saline-alkali land, and the application of the compound microbial fertilizer of the present invention can promote the fresh biomass and dry biomass of cotton, which are respectively increased by 19.92% and 24.25% compared with conventional fertilization. The number of cotton bolls per plant increased by 14.74%, the weight of single bolls increased by 12.38%, and the yield of seed cotton significantly increased by 41.43% compared with the conventional fertilization treatment. In addition, the application of compound microbial fertilizers decreased soil pH by 7.09%, EC by 25.83%, organic matter by 52.03%, alkaline nitrogen by 42.31%, available phosphorus by 46.46%, and available potassium by 32.45% compared with conventional fertilization.
下面对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明 中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
实施例中用到的节杆菌为Arthrobacter halodurans KJ-1，保藏编号为CCTCC NO：M 2021332，保藏时间为2021年4月6日，保藏单位为中国典型培养物保藏中心，保藏地址为中国.武汉.武汉大学；分类命名为Arthrobacter halodurans。The Arthrobacter halodurans KJ-1 used in the examples, the preservation number is CCTCC NO: M 2021332, the preservation time is April 6, 2021, the preservation unit is the China Type Culture Collection Center, and the preservation address is Wuhan, China .Wuhan University; Classified as Arthrobacter halodurans.
实施例1制备复合微生物肥料Embodiment 1 prepares compound microbial fertilizer
一种复合微生物肥料的制备方法，过程包括：A preparation method of compound microbial fertilizer, the process comprising:
1)制备节杆菌发酵液：将活化节杆菌菌株接种于发酵培养基中，37℃，180r/min摇床中发酵96h，得节杆菌发酵液；1) Preparation of Arthrobacter fermentation broth: Inoculate the activated Arthrobacter strain into the fermentation medium, ferment in a shaker at 37°C and 180r/min for 96h, and obtain the Arthrobacter fermentation broth;
2)称取：称取柠檬酸发酵尾液400ml、节杆菌发酵液600ml、尿素50g、磷酸一铵80g和硫酸钾40g；柠檬酸发酵尾液的pH为2.87，有机质含量为280.45g/L，腐殖酸3.45g/L，总氮120.34g/L，总磷3.48g/L和总钾20.67g/L；2) Weighing: Weigh 400ml of citric acid fermentation tail liquid, 600ml of Arthrobacter fermentation liquid, urea 50g, monoammonium phosphate 80g and potassium sulfate 40g; the pH of citric acid fermentation tail liquid is 2.87, and the organic matter content is 280.45g/L, Humic acid 3.45g/L, total nitrogen 120.34g/L, total phosphorus 3.48g/L and total potassium 20.67g/L;
3)初次混合：向柠檬酸发酵尾液中加入所述节杆菌发酵液，得发酵混合液；3) initial mixing: adding the Arthrobacter fermented liquid to the citric acid fermentation tail liquid to obtain a fermented mixed liquid;
4)二次混合：向所述发酵混合液中加入尿素、磷酸一铵、硫酸钾，搅拌至均一溶液，得混合液；4) Secondary mixing: adding urea, monoammonium phosphate, and potassium sulfate to the fermentation mixed solution, and stirring until a uniform solution was obtained to obtain a mixed solution;
5)调节pH值：向所述混合液中加入质量浓度为32％的氨水，调节pH值至5.5，得复合微生物肥料，复合微生物肥料中氮、磷、钾含量分别为2.33％、4.23％、3.06％。5) adjust the pH value: add the ammoniacal liquor that mass concentration is 32% to described mixed liquor, adjust pH value to 5.5, obtain compound microbial fertilizer, nitrogen, phosphorus, potassium content are respectively 2.33%, 4.23%, 2.33%, 4.23%, 3.06%.
实施例2Example 2
一种复合微生物肥料的制备方法，过程包括：A preparation method of compound microbial fertilizer, the process comprising:
1)制备节杆菌发酵液：将活化节杆菌菌株接种于发酵培养基中，37℃，180r/min摇床中发酵96h，得节杆菌发酵液；1) Preparation of Arthrobacter fermentation broth: Inoculate the activated Arthrobacter strain into the fermentation medium, ferment in a shaker at 37°C and 180r/min for 96h, and obtain the Arthrobacter fermentation broth;
2)称取：称取柠檬酸发酵尾液500ml、节杆菌发酵液500ml、尿素70g、磷酸一铵70g和硫酸钾70g；柠檬酸发酵尾液的pH为2.87，有机质含量为280.45g/L，腐殖酸3.45g/L，总氮120.34g/L，总磷3.48g/L和总钾20.67g/L；2) Weighing: Weigh 500ml of citric acid fermentation tail liquid, 500ml of Arthrobacter fermentation liquid, 70g of urea, 70g of monoammonium phosphate and 70g of potassium sulfate; the pH of citric acid fermentation tail liquid is 2.87, and the organic matter content is 280.45g/L, Humic acid 3.45g/L, total nitrogen 120.34g/L, total phosphorus 3.48g/L and total potassium 20.67g/L;
3)初次混合：向柠檬酸发酵尾液中加入所述节杆菌发酵液，得发酵混合液；3) initial mixing: adding the Arthrobacter fermented liquid to the citric acid fermentation tail liquid to obtain a fermented mixed liquid;
4)二次混合：向所述发酵混合液中加入尿素、磷酸一铵、硫酸钾，搅拌至均一溶液，得混合液；4) Secondary mixing: adding urea, monoammonium phosphate, and potassium sulfate to the fermentation mixed solution, and stirring until a uniform solution was obtained to obtain a mixed solution;
5)调节pH值：向所述混合液中加入质量浓度为31％的氨水，调节pH值至6.5，得复合微生物肥料，复合微生物肥料中氮、磷、钾含量分别为2.73％、6.17％、3.05％。5) adjust pH value: add the ammoniacal liquor that mass concentration is 31% to described mixed solution, adjust pH value to 6.5, obtain compound microbial fertilizer, nitrogen, phosphorus, potassium content are respectively 2.73%, 6.17%, 6.17% in the compound microbial fertilizer. 3.05%.
实施例3Example 3
一种复合微生物肥料的制备方法，过程包括：A preparation method of compound microbial fertilizer, the process comprising:
1)制备节杆菌发酵液：将活化节杆菌菌株接种于发酵培养基中，37℃，180r/min摇床中发酵96h，得节杆菌发酵液；1) Preparation of Arthrobacter fermentation broth: Inoculate the activated Arthrobacter strain into the fermentation medium, ferment in a shaker at 37°C and 180r/min for 96h, and obtain the Arthrobacter fermentation broth;
2)称取：称取柠檬酸发酵尾液600ml、节杆菌发酵液400ml、尿素80g、磷酸一铵50g和硫酸钾60g；柠檬酸发酵尾液的pH为2.87，有机质含量为280.45g/L，腐殖酸3.45g/L，总氮120.34g/L，总磷3.48g/L和总钾20.67g/L；2) Weighing: Weigh 600ml of citric acid fermentation tail liquid, 400ml of Arthrobacter fermentation liquid, urea 80g, monoammonium phosphate 50g and potassium sulfate 60g; the pH of citric acid fermentation tail liquid is 2.87, and the organic matter content is 280.45g/L, Humic acid 3.45g/L, total nitrogen 120.34g/L, total phosphorus 3.48g/L and total potassium 20.67g/L;
3)初次混合：向柠檬酸发酵尾液中加入所述节杆菌发酵液，得发酵混合液；3) initial mixing: adding the Arthrobacter fermented liquid to the citric acid fermentation tail liquid to obtain a fermented mixed liquid;
4)二次混合：向所述发酵混合液中加入尿素、磷酸一铵、硫酸钾，搅拌至均一溶液，得混合液；4) Secondary mixing: adding urea, monoammonium phosphate, and potassium sulfate to the fermentation mixed solution, and stirring until a uniform solution was obtained to obtain a mixed solution;
5)调节pH值：向所述混合液中加入质量浓度为35％的氨水，调节pH值至7.0，得复合微生物肥料，复合微生物肥料中氮、磷、钾含量分别为3.23％、2.87％、4.05％。5) adjust the pH value: add the ammoniacal liquor that mass concentration is 35% to described mixed solution, adjust pH value to 7.0, obtain compound microbial fertilizer, nitrogen, phosphorus, potassium content are respectively 3.23%, 2.87%, 2.87% in the compound microbial fertilizer. 4.05%.
实施例4Example 4
复合微生物肥料在非盐碱地番茄生长、产量及土壤改良中的应用。Application of compound microbial fertilizer in tomato growth, yield and soil improvement in non-saline-alkaline land.
在同等栽培条件下，设置小区进行对比实验，CK为常规大田施肥；处理1为施用本发明的实施例1的复合微生物肥料；处理2为施用本发明的实施例2的复合微生物肥料，处理3为施用本发明的实施例3的复合微生物肥料，小处理4为市面复合微生物肥料。番茄苗期，盛花期，盛果期分别施用100kg/亩复合微生物肥料，CK施用磷酸一铵100kg/亩。然后对不同处理番茄生物量、产量和土壤基础养分进行检测，试验于新疆石河子市石总场进行，供试作物为番茄，供试品种为1612，种植方式为覆膜滴灌，1膜2管2行配置，株距40cm，行距60cm，理论株数5.2*104株·hm
-2。总灌水量150m
3亩，灌水8次，随水施肥6次。
Under the same cultivation conditions, plots are set up and carried out comparative experiments, CK is conventional field fertilization; processing 1 is the application of the compound microbial fertilizer of embodiment 1 of the present invention; processing 2 is the application of the compound microbial fertilizer of embodiment 2 of the present invention, processing 3 For using the compound microbial fertilizer of embodiment 3 of the present invention, small treatment 4 is the compound microbial fertilizer on the market. 100kg/mu compound microbial fertilizer was applied at tomato seedling stage, full flower stage and full fruit stage respectively, and 100kg/mu of monoammonium phosphate was applied to CK. Then the tomato biomass, yield and soil basic nutrients of different treatments were detected. The test was carried out in Shizong Field, Shihezi City, Xinjiang. The test crop was tomato, and the test variety was 1612. The planting method was film-covered drip irrigation, 1 film 2 tubes 2 Row configuration, plant spacing 40cm, row spacing 60cm, theoretical plant number 5.2*104 plants·hm -2 . The total irrigation volume is 150m 3 mu, 8 times of irrigation, and 6 times of fertilization with water.
番茄生物量的测定：Determination of tomato biomass:
番茄初花后，挖取每小区1m*1m＝1m
2内植株，迅速带回实验室，测定番茄鲜生物量，然后在105℃杀青30min，70℃烘至恒重，测定干生物量。结果如表1所示；
After the first flowering of tomato, dig out the plants within 1m*1m= 1m2 of each plot, and quickly bring them back to the laboratory to measure the fresh biomass of tomatoes, then kill them at 105°C for 30min, bake them at 70°C to constant weight, and measure the dry biomass. The result is shown in Table 1;
表1.不同处理对番茄生物量的影响Table 1. Effects of different treatments on tomato biomass
由表1可知，施用实施例1复合微生物肥料可促进番茄鲜生物量、干生物量，较常规施肥分别增加5.24％，8.66％。施用实施例2复合微生物肥料可促进番茄鲜生物量、干生物量，较常规施肥分别增加18.78％，22.69％。施用实施例3复合微生物肥料可促进番茄鲜生物量、干生物量，较常规施肥分别增加8.34％，15.26％。市面复合微生物肥料可促进番茄鲜生物量、干生物量，较常规施肥分别增加16.24％，12.51％。以上结果表明施用实施例2复合微生物肥料对促进番茄鲜生物量、干生物量最佳。It can be seen from Table 1 that the application of the compound microbial fertilizer of Example 1 can promote the fresh biomass and dry biomass of tomato, which are respectively increased by 5.24% and 8.66% compared with conventional fertilization. Applying the compound microbial fertilizer of Example 2 can promote the fresh biomass and dry biomass of tomato, which are respectively increased by 18.78% and 22.69% compared with conventional fertilization. Applying the compound microbial fertilizer of Example 3 can promote the fresh biomass and dry biomass of tomato, which are respectively increased by 8.34% and 15.26% compared with conventional fertilization. The compound microbial fertilizers in the market can promote the fresh biomass and dry biomass of tomato, which are increased by 16.24% and 12.51% respectively compared with conventional fertilization. The above results show that applying the compound microbial fertilizer of Example 2 is the best for promoting tomato fresh biomass and dry biomass.
番茄产量的测定：Determination of tomato yield:
于番茄采收期，随机取3个1m*1m＝1m
2的样点，采收番茄，记录果数和重量，从而估测番茄产量。每亩番茄产量＝(单株果数*单果重*每亩株数*相关系数)/1000。结果见表2；
During the tomato harvest period, randomly select 3 sampling points of 1m*1m=1m2, harvest the tomatoes, record the fruit number and weight, so as to estimate the tomato yield. Tomato yield per mu = (number of fruit per plant * weight of single fruit * number of plants per mu * correlation coefficient)/1000. The results are shown in Table 2;
表2施有机液体肥番茄产量及产量构成因素Table 2 Application of organic liquid fertilizer tomato yield and yield components
由表2可知，施用实施例1复合微生物肥料对番茄产量有显著促进作用，较未施用处理单果重增加2.66％，产量增加4.33％。施用实施例2复合微生物肥料对番茄产量有显著促进作用，较未施用处理单果重增加18.90％，产量增加13.11％。施用实施例3复合微生物肥料对番茄产量有显著促进作用，较未施用处理单果重增加15.18％，产量增加4.98％。施用实施例3复合微生物肥料对番茄产量有显著促进作用，较未施用处理单果重增加15.18％，产量增加4.98％。施用市面复合微生物肥料对番茄产量有显著促进作用，较未施用处理单果重增加17.41％，产量增加4.71％。以上结果表明施用实施例2复合微生物肥料提高番茄单果重及产量最大。It can be seen from Table 2 that the application of the compound microbial fertilizer of Example 1 has a significant promotion effect on the tomato yield, and the single fruit weight increased by 2.66% and the yield increased by 4.33% compared with the unapplied treatment. The application of the compound microbial fertilizer of Example 2 has a significant effect on promoting the yield of tomato, and the single fruit weight increased by 18.90% and the yield increased by 13.11% compared with the unapplied treatment. The application of the compound microbial fertilizer of Example 3 has a significant promotion effect on the tomato yield, and the single fruit weight increased by 15.18% and the yield increased by 4.98% compared with the unapplied treatment. The application of the compound microbial fertilizer of Example 3 has a significant promotion effect on the tomato yield, and the single fruit weight increased by 15.18% and the yield increased by 4.98% compared with the unapplied treatment. The application of compound microbial fertilizers in the market can significantly promote the yield of tomato, which increases the single fruit weight by 17.41% and the yield by 4.71% compared with the non-application treatment. The above results show that applying the compound microbial fertilizer of Example 2 improves the single fruit weight and yield of tomato to the maximum.
土壤样品理化性质测定Determination of physical and chemical properties of soil samples
于番茄拉秧期采集未施用本发明复合微生物肥料与施用本发明复合微生物肥料土样，风干后先后过1mm和0.15mm筛。先后对土壤进行有机质测定(重铬酸钾容量法)、pH值测定、碱解氮测定(采用碱解扩散法)、速效磷测定(采用钼锑抗比色法)、速效钾测定(采用火焰光度计法)，结果见表3；The soil samples not applied with the compound microbial fertilizer of the present invention and applied with the compound microbial fertilizer of the present invention were collected at the tomato seedling pulling stage, passed through 1 mm and 0.15 mm sieves successively after air-drying. The soil was successively tested for organic matter (potassium dichromate volumetric method), pH value, alkaline hydrolysis nitrogen (by alkaline hydrolysis diffusion method), available phosphorus (by molybdenum antimony anti-colorimetric method), available potassium (by flame Photometer method), the results are shown in Table 3;
表3施有机液体肥番茄土壤理化指标影响Table 3 Effect of organic liquid fertilizer on tomato soil physical and chemical indicators
由表3可知，施用实施例1复合微生物肥料可以降低土壤的pH值，降低量为1.12％，且可增加速效钾、速效磷、有机质、碱解氮含量，增加量分别为2.46％、4.02％、13.65％、5.61％。施用实施例2复合微生物肥料可以降低土壤的pH值，降低量为1.52％，且可增加速效钾、速效磷、有机质、碱解氮含量，增加量分别为8.13％、18.01％、17.64％、16.97％。施用实施例3复合微生物肥料可以降低土壤的pH值，降低量为0.25％，且可增加速效钾、速效磷、有机质、碱解氮含量，增加量分别为5.80％、13.17％、7.58％、5.80％。施用市面复合微生物肥料可以降低土壤的pH值，降低量为0.10％，且可增加速效钾、速效磷、有机质、碱解氮含量，增加量分别为4.84％、14.91％、6.87％、10.68％。说明施用复合微生物肥料后能够提高土壤的速效养分，具有培肥地力、改善土壤质量的作用，其中施用实施例2复合微生物肥料效果最佳。It can be seen from Table 3 that the application of the compound microbial fertilizer of Example 1 can reduce the pH value of the soil by 1.12%, and can increase the contents of available potassium, available phosphorus, organic matter and alkaline nitrogen by 2.46% and 4.02% respectively , 13.65%, 5.61%. Application of Example 2 compound microbial fertilizer can reduce the pH value of the soil, the reduction is 1.52%, and can increase the content of available potassium, available phosphorus, organic matter, and alkaline nitrogen, and the increase is respectively 8.13%, 18.01%, 17.64%, 16.97% %. Application of Example 3 compound microbial fertilizer can reduce the pH value of the soil, the reduction is 0.25%, and can increase the content of available potassium, available phosphorus, organic matter, and alkaline nitrogen, and the increase is respectively 5.80%, 13.17%, 7.58%, 5.80% %. The application of commercially available compound microbial fertilizers can reduce the pH value of the soil by 0.10%, and increase the content of available potassium, available phosphorus, organic matter, and alkaline nitrogen by 4.84%, 14.91%, 6.87%, and 10.68%, respectively. Illustrate that the available nutrients of the soil can be improved after applying the compound microbial fertilizer, which has the effects of fertilizing soil fertility and improving soil quality, and the effect of applying the compound microbial fertilizer of Example 2 is the best.
实施例5Example 5
复合微生物肥料在盐碱地棉花生长、产量及土壤改良中的应用Application of Compound Microbial Fertilizer in Cotton Growth, Yield and Soil Improvement in Saline-alkali Land
在同等栽培条件下，设置小区进行对比实验，小区基础理化性质为pH：8.91，EC：3.41ms/cm，有机质：13.28g/kg，碱解氮：13.92mg/kg，速效磷20.92mg/kg，速效钾：110.92mg/kg。Under the same cultivation conditions, plots were set up for comparative experiments. The basic physical and chemical properties of the plots are pH: 8.91, EC: 3.41ms/cm, organic matter: 13.28g/kg, alkaline nitrogen: 13.92mg/kg, available phosphorus: 20.92mg/kg , Available potassium: 110.92mg/kg.
对棉花生物量、产量和土壤基础养分进行检测。每个处理3个重复，面积为20亩，具体处理如下；Cotton biomass, yield and soil basal nutrients were tested. Each treatment is repeated 3 times, and the area is 20 mu, and the specific treatment is as follows;
设置小区进行对比实验，CK为常规大田施肥；处理1为施用本发明的实施例1的复合微生物肥料；处理2为施用本发明的实施例2的复合微生物肥料，处理3为施用本发明的实施例3的复合微生物肥料，处理4为市面复合微生物肥料。番茄苗期，盛花期，盛果期分别施用100kg/亩复合微生物肥料，CK施用磷酸一铵100kg/亩。棉花采用覆膜种植，机械点播，1膜3管6行配置，总灌水量350m
3/亩，灌水8次，随水施肥7次。
Set up plot and carry out comparative experiment, CK is conventional field fertilization; Process 1 is to use the compound microbial fertilizer of embodiment 1 of the present invention; Process 2 is to use the compound microbial fertilizer of embodiment 2 of the present invention, and process 3 is to use the implementation of the present invention The compound microbial fertilizer of example 3, processing 4 is the compound microbial fertilizer on the market. 100kg/mu compound microbial fertilizer was applied at tomato seedling stage, full flower stage and full fruit stage respectively, and 100kg/mu of monoammonium phosphate was applied to CK. Cotton is planted with plastic film, mechanical on-demand, 1 film, 3 tubes and 6 rows, with a total irrigation volume of 350m 3 /mu, 8 times of irrigation, and 7 times of fertilization with water.
棉花生物量的测定方法：Cotton biomass determination method:
棉花初花后，挖取每小区1m*2.2m＝2.2m
2棉株，迅速带回实验室，测定棉花鲜生物量，然后在105℃杀青30min，70℃烘至恒重，测定干生物量；结果如表4；
After the initial flowering of cotton, dig out 1m*2.2m=2.2m 2 cotton plants per plot, and quickly bring them back to the laboratory to measure the fresh biomass of cotton, then kill them at 105°C for 30min, dry them at 70°C to constant weight, and measure the dry biomass ; The results are shown in Table 4;
表4不同处理对棉花生物量的影响Table 4 Effects of different treatments on cotton biomass
由表4可知，施用实施例1复合微生物肥料可促进棉花鲜生物量、干生物量，较常规施肥分别增加3.87％，8.93％。施用实施例2复合微生物肥料可促进棉花鲜生物量、干生物量，较常规施肥分别增加19.92％，24.25％。施用实施例3复合微生物肥料可促进棉花鲜生物量、干生物量，较常规施肥分别增加15.91％，19.66％。施用市面复合微生物肥料可促进棉花鲜生物量、干生物量，较常规施肥分别增加17.70％，18.46％。以上结果表明施用实施例2复合微生物肥料效果最佳。It can be seen from Table 4 that the application of the compound microbial fertilizer of Example 1 can promote the fresh biomass and dry biomass of cotton, which are respectively increased by 3.87% and 8.93% compared with conventional fertilization. Applying the compound microbial fertilizer of Example 2 can promote the fresh biomass and dry biomass of cotton, which are respectively increased by 19.92% and 24.25% compared with conventional fertilization. Applying the compound microbial fertilizer of Example 3 can promote the fresh biomass and dry biomass of cotton, which are respectively increased by 15.91% and 19.66% compared with conventional fertilization. The application of commercially available compound microbial fertilizers can increase the fresh biomass and dry biomass of cotton by 17.70% and 18.46%, respectively, compared with conventional fertilization. The above results show that the effect of using the compound microbial fertilizer of Example 2 is the best.
棉花产量的测定Determination of Cotton Yield
棉花吐絮后，每小区随机取样1m*2.2m＝2.2m2，测定其株数、铃数，每小区分上、中、下层采收共100铃，晒干测定平均单铃重。每亩籽棉产量＝(铃数*平均铃重)*小区株数*300*0.85(测产系数)/1000；结果见表5；After cotton bolling, randomly sample 1m*2.2m=2.2m2 in each plot, measure the number of plants and bolls, harvest a total of 100 bolls from the upper, middle, and lower layers of each plot, and measure the average single boll weight after drying. Yield per mu of seed cotton=(number of bolls*average boll weight)*number of plots*300*0.85 (measurement coefficient)/1000; the results are shown in Table 5;
表5不同处理对棉花产量的影响Table 5 Effects of different treatments on cotton yield
由表5可知，施用实施例1复合微生物肥料处理较常规施肥处理棉花单株铃数增加5.07％，单铃重增加7.34％，籽棉产量显著增加21.10％。施用实施例2复合微生物肥料处理较常规施肥处理棉花单株铃数增加14.74％，单铃重增加12.38％，籽棉产量显著增加41.43％。施用实施例3复合微生物肥料处理较常规施肥处理棉花单株铃数增加10.83％，单铃重增加9.86％，籽棉产量显著增加34.49％。施用市面复合微生物肥料处理较常规施肥处理棉花单株铃数增加10.37％，单铃重增加8.72％，籽棉产量显著增加35.48％。说明施用复合微生物肥料后能够增加棉花单株铃数、单铃重，从而增加籽棉产量，其中施用实施例2复合微生物肥料处理对花单株铃数、单铃重及籽棉产量增加最大。It can be seen from Table 5 that the number of bolls per plant of cotton increased by 5.07%, the weight of single bolls increased by 7.34%, and the yield of seed cotton significantly increased by 21.10% compared with the conventional fertilization treatment. The number of bolls per plant of cotton increased by 14.74%, the weight of single bolls increased by 12.38%, and the yield of seed cotton significantly increased by 41.43% compared with the conventional fertilization treatment. The number of bolls per plant of cotton increased by 10.83%, the weight of single bolls increased by 9.86%, and the yield of seed cotton significantly increased by 34.49% compared with the conventional fertilization treatment. The number of cotton bolls per plant increased by 10.37%, the weight of single bolls increased by 8.72%, and the yield of seed cotton significantly increased by 35.48% under the application of commercial compound microbial fertilizers compared with conventional fertilization treatments. It shows that the application of compound microbial fertilizer can increase the number of bolls per plant of cotton and the weight of single boll, thereby increasing the yield of seed cotton. Wherein, the number of bolls per plant of flowers, the weight of single boll and the yield of seed cotton increased by the application of compound microbial fertilizer in Example 2 are the largest.
土壤样品的测定Determination of soil samples
棉花花铃期采集距滴灌带距离10cm附近土层土样，取3点进行混合样品，风干后先后过1mm和0.15mm筛。然后对土壤进行基本理化性质测定：有机质采用重铬酸钾容量法测定、pH和电导率采用酸碱仪测定、碱解氮采用碱解扩散法测定、速效磷采用钼锑抗比色法测定、速效钾采用火焰光度计法测定。棉花花铃期，对试验地土壤进行取样测定，结果如表6所示；Collect soil samples from the soil layer near the drip irrigation belt at a distance of 10cm during the flowering and boll stage of cotton, take 3 points for mixed samples, and pass through 1mm and 0.15mm sieves successively after air-drying. Then the basic physical and chemical properties of the soil were measured: organic matter was measured by potassium dichromate volumetric method, pH and electrical conductivity were measured by acid-base meter, alkaline nitrogen was measured by alkaline solution diffusion method, available phosphorus was measured by molybdenum antimony anti-colorimetric method, Available potassium was determined by flame photometry. At the flowering and boll stage of cotton, the soil of the test site was sampled and determined, and the results are shown in Table 6;
表6.不同处理对棉花盛铃期土壤的各项指标的影响Table 6. Effects of different treatments on various indicators of cotton soil at full boll stage
由表6可以看出，施用实施例1复合微生物肥料处理较常规施肥处理土壤pH降低3.93％，EC降低16.13％，有机质提高8.13％、碱解氮提高17.89％、速效磷显著提高12.62％、速效钾提高9.95％。施用实施例2复合微生物肥料 处理较常规施肥处理土壤pH降低7.09％，EC降低25.83％，有机质提高52.03％、碱解氮提高42.31％、速效磷显著提高46.46％、速效钾提高32.45％。施用实施例3复合微生物肥料处理较常规施肥处理土壤pH降低2.58％，EC降低15.24％，有机质提高23.42％、碱解氮提高34.27％、速效磷显著提高36.95％、速效钾提高19.33％。施用市面复合微生物肥料处理较常规施肥处理土壤pH降低2.58％，EC降低5.05％，有机质提高18.15％、碱解氮提高23.28％、速效磷显著提高21.80％、速效钾提高19.51％。说明施用复合微生物肥料后能够提高盐碱地土壤的速效养分并降低pH、EC，具有培肥地力、改善土壤质量的作用，其中实施例2复合微生物肥料对盐碱地改良效果最好。As can be seen from Table 6, the application of the compound microbial fertilizer in Example 1 reduces the soil pH by 3.93% compared with the conventional fertilization treatment, EC reduces by 16.13%, organic matter increases by 8.13%, alkaline hydrolysis nitrogen increases by 17.89%, available phosphorus significantly increases by 12.62%, and available Potassium boosted by 9.95%. Application Example 2 compound microbial fertilizer treatment compared with conventional fertilization treatment soil pH decreased 7.09%, EC decreased 25.83%, organic matter increased 52.03%, alkaline hydrolysis nitrogen increased 42.31%, available phosphorus significantly increased 46.46%, available potassium increased 32.45%. Application Example 3 compound microbial fertilizer treatment compared with conventional fertilization treatment soil pH decreased by 2.58%, EC decreased by 15.24%, organic matter increased by 23.42%, alkaline hydrolysis nitrogen increased by 34.27%, available phosphorus significantly increased by 36.95%, and available potassium increased by 19.33%. Compared with conventional fertilization, the application of commercially available compound microbial fertilizers decreased soil pH by 2.58%, EC decreased by 5.05%, organic matter increased by 18.15%, alkaline nitrogen increased by 23.28%, available phosphorus increased significantly by 21.80%, and available potassium increased by 19.51%. It shows that the application of compound microbial fertilizer can improve the available nutrients of saline-alkali soil and reduce pH, EC, and has the effect of fertilizing soil fertility and improving soil quality. Among them, the compound microbial fertilizer of Example 2 has the best improvement effect on saline-alkali soil.
本说明书中各个实施例采用递进的方式描述，每个实施例重点说明的都是与其他实施例的不同之处，各个实施例之间相同相似部分互相参见即可。Each embodiment in this specification is described in a progressive manner, each embodiment focuses on the difference from other embodiments, and the same and similar parts of each embodiment can be referred to each other.
对所公开的实施例的上述说明，使本领域专业技术人员能够实现或使用本发明。对这些实施例的多种修改对本领域的专业技术人员来说将是显而易见的，本文中所定义的一般原理可以在不脱离本发明的精神或范围的情况下，在其它实施例中实现。因此，本发明将不会被限制于本文所示的这些实施例，而是要符合与本文所公开的原理和新颖特点相一致的最宽的范围。The above description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the present invention will not be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
- 一种复合微生物肥料，其特征在于，包括柠檬酸发酵尾液、节杆菌发酵液、尿素、磷酸一铵和硫酸钾；且各组分的配比:(400～600)mL:(400～600)mL:(50～80)g:(50～80)g:(40～60)g；所述复合微生物肥料以氨水调节pH值至5.5-7.0。A compound microbial fertilizer is characterized in that it comprises citric acid fermentation tail liquid, Arthrobacter fermentation liquid, urea, monoammonium phosphate and potassium sulfate; and the proportioning of each component: (400～600)mL:(400～600 ) mL: (50-80) g: (50-80) g: (40-60) g; the compound microbial fertilizer uses ammonia water to adjust the pH value to 5.5-7.0.
- 根据权利要求1所述的一种复合微生物肥料，其特征在于，所述柠檬酸发酵尾液中有机质含量为280-300g/L。A kind of compound microbial fertilizer according to claim 1, is characterized in that, the organic matter content in the citric acid fermentation tail liquid is 280-300g/L.
- 根据权利要求1所述的一种复合微生物肥料，其特征在于，所述节杆菌发酵液中的活菌数≥0.5×10 8cfu/ml。 A compound microbial fertilizer according to claim 1, characterized in that the number of viable bacteria in the Arthrobacter fermentation broth is ≥0.5×10 8 cfu/ml.
- 根据权利要求1所述的一种复合微生物肥料，其特征在于，所述氨水的质量浓度为31-35％。A kind of compound microbial fertilizer according to claim 1, is characterized in that, the mass concentration of described ammoniacal liquor is 31-35%.
- 根据权利要求1-4任一所述的一种复合微生物肥料的制备方法，其特征在于，包括下述步骤：The preparation method of a kind of composite microbial fertilizer according to any one of claims 1-4, is characterized in that, comprises the steps:1)制备节杆菌发酵液：将活化节杆菌菌株接种于发酵培养基中，37℃，180r/min摇床中发酵96h，得节杆菌发酵液；1) Preparation of Arthrobacter fermentation broth: Inoculate the activated Arthrobacter strain into the fermentation medium, ferment in a shaker at 37°C and 180r/min for 96h, and obtain the Arthrobacter fermentation broth;2)称取：按照体积质量比称取柠檬酸发酵尾液、节杆菌发酵液、氨水、尿素、磷酸一铵和硫酸钾；2) Weighing: Weighing citric acid fermentation tail liquid, Arthrobacter fermentation liquid, ammonia water, urea, monoammonium phosphate and potassium sulfate according to volume to mass ratio;3)初次混合：向柠檬酸发酵尾液中加入所述节杆菌发酵液，得发酵混合液；3) initial mixing: adding the Arthrobacter fermented liquid to the citric acid fermentation tail liquid to obtain a fermented mixed liquid;4)二次混合：向所述发酵混合液中加入尿素、磷酸一铵、硫酸钾，搅拌至均一溶液，得混合液；4) Secondary mixing: adding urea, monoammonium phosphate, and potassium sulfate to the fermentation mixed solution, and stirring until a uniform solution was obtained to obtain a mixed solution;5)调节pH值：向所述混合液中加入氨水，调节pH值至5.5-7.0，得复合微生物肥料。5) Adjusting the pH value: adding ammonia water to the mixed solution to adjust the pH value to 5.5-7.0 to obtain a compound microbial fertilizer.
- 根据权利要求5所述的一种复合微生物肥料的制备方法，其特征在于，所述发酵培养基包括酵母提取物、胰蛋白胨和蒸馏水，且，三者的质量体积比为：1g:2g：200ml。The preparation method of a kind of compound microbial fertilizer according to claim 5, is characterized in that, described fermentation medium comprises yeast extract, tryptone and distilled water, and, the mass volume ratio of the three is: 1g:2g:200ml .
- 根据权利要求5所述的一种复合微生物肥料的制备方法，其特征在于，所述发酵培养基的pH值为7.5-7.8。The preparation method of a kind of compound microbial fertilizer according to claim 5, is characterized in that, the pH value of described fermentation medium is 7.5-7.8.
- 根据权利要求5所述的一种复合微生物肥料的制备方法，其特征在于，制备得到的复合微生物肥料中氮、磷、钾含量分别为2.73％、6.17％、3.05％。The method for preparing a compound microbial fertilizer according to claim 5, characterized in that the contents of nitrogen, phosphorus and potassium in the prepared compound microbial fertilizer are 2.73%, 6.17%, and 3.05%, respectively.
- 权利要求5-8任一所述的制备方法制备得到的复合微生物肥料在提高作物产量、促进植物生长中的应用。The application of the compound microbial fertilizer prepared by the preparation method described in any one of claims 5-8 in improving crop yield and promoting plant growth.
- 权利要求5-8任一所述的制备方法制备得到的复合微生物肥料在改良盐碱地土壤中的应用。Application of the compound microbial fertilizer prepared by the preparation method described in any one of claims 5-8 in improving saline-alkali soil.
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