WO2023241315A1 - 一种棕榈仁浓缩蛋白、其制备方法及生产系统 - Google Patents
一种棕榈仁浓缩蛋白、其制备方法及生产系统 Download PDFInfo
- Publication number
- WO2023241315A1 WO2023241315A1 PCT/CN2023/095626 CN2023095626W WO2023241315A1 WO 2023241315 A1 WO2023241315 A1 WO 2023241315A1 CN 2023095626 W CN2023095626 W CN 2023095626W WO 2023241315 A1 WO2023241315 A1 WO 2023241315A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- palm kernel
- protein concentrate
- embryo
- kernel protein
- pieces
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 70
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 70
- 239000012141 concentrate Substances 0.000 title claims abstract description 56
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 54
- 238000000034 method Methods 0.000 claims abstract description 29
- 238000002791 soaking Methods 0.000 claims abstract description 23
- 239000002904 solvent Substances 0.000 claims abstract description 17
- 238000005238 degreasing Methods 0.000 claims abstract description 15
- 239000003960 organic solvent Substances 0.000 claims abstract description 14
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 13
- 238000000605 extraction Methods 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 6
- 210000001161 mammalian embryo Anatomy 0.000 claims description 75
- 238000005096 rolling process Methods 0.000 claims description 11
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 claims description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 claims description 8
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 8
- 238000003825 pressing Methods 0.000 claims description 7
- 239000003208 petroleum Substances 0.000 claims description 4
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- 235000019764 Soybean Meal Nutrition 0.000 abstract description 8
- 239000004455 soybean meal Substances 0.000 abstract description 8
- 239000002994 raw material Substances 0.000 abstract description 4
- 241001465754 Metazoa Species 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- -1 i.e. Substances 0.000 abstract 1
- 239000013557 residual solvent Substances 0.000 abstract 1
- 101100291369 Mus musculus Mip gene Proteins 0.000 description 89
- 101150116466 PALM gene Proteins 0.000 description 89
- 235000018102 proteins Nutrition 0.000 description 52
- 235000012054 meals Nutrition 0.000 description 15
- 239000003921 oil Substances 0.000 description 15
- 235000019198 oils Nutrition 0.000 description 15
- 150000001413 amino acids Chemical class 0.000 description 12
- 241000287828 Gallus gallus Species 0.000 description 7
- 229920002472 Starch Polymers 0.000 description 7
- 235000013330 chicken meat Nutrition 0.000 description 7
- 150000004676 glycans Chemical class 0.000 description 7
- 229920001282 polysaccharide Polymers 0.000 description 7
- 239000005017 polysaccharide Substances 0.000 description 7
- 239000008107 starch Substances 0.000 description 7
- 235000019698 starch Nutrition 0.000 description 6
- 239000004475 Arginine Substances 0.000 description 5
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 5
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 5
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 5
- 229930182817 methionine Natural products 0.000 description 5
- 108010073032 Grain Proteins Proteins 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000000638 solvent extraction Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 235000020776 essential amino acid Nutrition 0.000 description 3
- 239000003797 essential amino acid Substances 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 239000003346 palm kernel oil Substances 0.000 description 3
- 235000019865 palm kernel oil Nutrition 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 235000019784 crude fat Nutrition 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 230000002009 allergenic effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Definitions
- the invention relates to the technical field of palm kernel protein concentrate preparation, and in particular to a palm kernel protein concentrate, its preparation method and production system.
- the object of the present invention is to provide a palm kernel protein concentrate, its preparation method and production system.
- the palm kernel protein concentrate method Through the design of the palm kernel protein concentrate method, the crude fiber content of palm kernel meal obtained by mechanical pressing or solvent extraction in the existing technology can be solved. High, low protein content, low commercial value and other issues.
- the invention provides a method for preparing palm kernel protein concentrate, which includes the following preparation steps:
- the rolling process is carried out at 50-70°C.
- the drying temperature of the green sheet is 50-70°C.
- the time for soaking the dried embryo pieces in the organic solvent is 2-5 hours.
- the mass content of protein in the obtained palm kernel protein concentrate is ⁇ 40%.
- the concentration of ethanol is 75%.
- the present invention also provides a palm kernel protein concentrate obtained based on the method for preparing palm kernel protein concentrate as described in any one of the above.
- the method for preparing palm kernel protein concentrate proposed by the present invention can obtain palm kernel protein concentrate, which nearly doubles the protein content of traditional palm kernel meal.
- the fat content is reduced from the original 9% to 9% through solvent extraction and defatting. 1%; the essential amino acid content is greatly improved compared with traditional palm kernel meal, and the total amino acid content is more than doubled. It can be used as a substitute for soybean meal, the main animal feed source.
- the embryo pieces are dried at low temperature, and the dried embryo pieces are soaked in organic solvents for degreasing and oil extraction. After degreasing and oil extraction, the embryo pieces to be desaccharified are obtained;
- the embryo pieces to be desugared are first subjected to ultrasonic treatment. After the ultrasonic treatment, the embryo pieces to be desugared are soaked in an ethanol solvent to perform desugaring treatment. After soaking for a certain period of time, the desugared embryo pieces are obtained;
- the desugared embryo pieces are soaked in water for desugaring treatment, and the soaking time is 0.5-3 hours.
- the billet rolling process is carried out at 50-70°C.
- the drying temperature of the green sheet is 50-70°C.
- the thickness of the green sheet is 0.3-1.0mm.
- the time for soaking the dried embryo pieces in the organic solvent is 2-5 hours.
- the organic solvent is at least one of n-hexane, petroleum ether, n-pentane, n-heptane, and cyclohexane;
- the mass content of protein in the obtained palm kernel protein concentrate is ⁇ 40%.
- the concentration of ethanol was 75%.
- a method for preparing palm kernel protein concentrate includes the following preparation steps:
- the desugared embryo pieces are extruded to remove residual ethanol and then dried at low temperature to obtain concentrated palm kernel protein.
- the crude protein of the sample was determined using the Kjeldahl nitrogen determination method (GB/T 6432-1994), acid hydrolysis crude fat determination method (GB/T 6433-2006), and 550°C ignition method (GB/T 6438-2007). , crude fat and crude ash content; amino acid content was measured using the national standard GB/T 18246-2000 method.
- the components of the obtained palm kernel protein concentrate are shown in Table 1.
- the kernel shell is separated by a shelling machine to remove the hard shell, and the lignin fiber component is removed to the greatest extent through the fine separation process to ensure the protein content of the product during subsequent processing.
- the palm kernel shell separator adopts the palm kernel shell separator produced by Henan Guorui Oil Engineering Technology Co., Ltd.
- the embryo-tying machine adopts the embryo-tying machine produced by Shandong Bangteng Machinery Equipment Co., Ltd.
- a method for preparing palm kernel protein concentrate includes the following preparation steps:
- the desugared embryo pieces are extruded to remove residual ethanol and then dried at low temperature to obtain concentrated palm kernel protein.
- the organic solvent in this embodiment is petroleum ether
- the components of the palm kernel protein concentrate and the amino acid utilization rate of the palm kernel protein concentrate obtained in this example are shown in Table 1 and Table 2.
- a method for preparing palm kernel protein concentrate includes the following preparation steps:
- the desugared embryo pieces are extruded to remove residual ethanol and then dried at low temperature to obtain concentrated palm kernel protein.
- the thickness of the green sheet in this example is 1.0mm;
- the organic solvent in this embodiment is n-pentane.
- the components of the palm kernel protein concentrate and the amino acid utilization rate of the palm kernel protein concentrate obtained in this example are shown in Table 1 and Table 2.
- Comparative Example 1 selected palm kernel meal (purchased from Tianjin Jinyue Agricultural Products Co., Ltd.).
- Comparative Example 2 selected peeled soybean meal (purchased from Qingdao Bohai Technology Co., Ltd.).
- Palm kernel meal has high arginine and methionine content, but after the new palm kernel protein concentrate has been greatly improved, its arginine and methionine content is similar to that of soybean meal, but other amino acid levels are still lower than soybean meal.
- the amino acid content of the palm kernel protein concentrate of Example 1 accounts for 88% of the crude protein, which is similar to soybean meal. This ratio is much higher than the palm kernel meal of Comparative Example 1 (83%).
- the amino acid utilization rate of chickens in the processed palm kernel protein concentrate was slightly higher than that of palm meal, among which the utilization rate of arginine, histidine, lysine, leucine, methionine and phenylalanine are above 90%.
- the preparation process of palm kernel protein concentrate in this example is the same as that in Example 1. The only difference is that the embryo pieces to be desugared are soaked in an ethanol solvent. The ethanol concentration is 75%, and the desugaring treatment is performed. The soaking time is 0.5 h. The non-starch polysaccharide content in the embryo pieces is shown in Table 3.
- the preparation process of palm kernel protein concentrate in this example is the same as that in Example 1. The only difference is that the embryo pieces to be desugared are soaked in an ethanol solvent. The ethanol concentration is 75%, and the desugaring treatment is performed. The soaking time is 1 hour. The non-starch polysaccharide content in embryonic slices is shown in Table 3.
- the preparation process of palm kernel protein concentrate in this example is the same as that in Example 1. The only difference is that the embryo pieces to be desugared are soaked in an ethanol solvent. The ethanol concentration is 75%, and desugaring is performed. The soaking time is 2 hours. The non-starch polysaccharide content in embryonic slices is shown in Table 3.
- the preparation process of palm kernel protein concentrate in this example is the same as that in Example 1. The only difference is that the embryo pieces to be desugared are soaked in an ethanol solvent. The ethanol concentration is 75%, and the desugaring treatment is performed. The soaking time is 3 hours. The non-starch polysaccharide content in embryonic slices is shown in Table 3.
- Example 4 Compared with Example 1, Example 4, Example 5, Example 6 and Example 7 only changed the soaking time of the embryo pieces to be desaccharified in ethanol during the desugaring process, which can realize the extraction of soluble non-starch polysaccharides. By controlling the rate, the soluble non-starch polysaccharide content in palm kernel protein concentrate products can be controlled according to different product requirements, thereby obtaining different series of palm kernel protein concentrate products.
- Table 1 Essential amino acid composition (%) of palm kernel protein concentrate, palm kernel meal and soybean meal
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Fodder In General (AREA)
Abstract
一种棕榈仁浓缩蛋白、其制备方法及生产系统。制备包括：仁壳分离，获得棕榈果仁；浸泡水中，软化后低温轧胚获得胚片；低温烘干，烘干后的胚片浸泡在有机溶剂中脱脂提油获得待脱糖胚片；将待脱糖胚片经超声处理后，浸泡于乙醇溶剂中，脱糖获得脱糖的胚片；将脱糖胚片经挤压工艺去除残留的溶剂后低温烘干，获得棕榈仁浓缩蛋白。制备的棕榈仁浓缩蛋白蛋白含量高、可以作为动物性饲料原料豆粕的替代品。
Description
本发明涉及棕榈仁浓缩蛋白制备技术领域，尤其是涉及一种棕榈仁浓缩蛋白、其制备方法及生产系统。
[根据细则26改正 18.08.2023]
全球非粮蛋白种类丰富，但技术研发严重不足，限制了其在饲料中的应用。因此，突破新型非粮蛋白例如棕榈等大宗非粮蛋白资源提质、增效、改性等预处理和深加工关键技术，是解决当前我国饲料蛋白原料困境的有效途径之一。棕榈是世界上植物油产量最大的植物品种，全球棕榈产量约7000万吨，棕榈仁是棕榈果肉去除后的种籽，主要由坚硬的棕榈仁壳和棕榈果仁两部分组成。棕榈果仁含油45％-50％，棕榈果仁是重要的榨油原料，可用于生产棕榈仁油，其经压榨或溶剂萃取法可获得压榨棕榈仁油，残渣为棕榈仁粕，全球产量达千万吨。机械压榨法投入低，操作简便，是目前最为主流的加工方式，因此市面上的棕榈仁粕基本都由机械压榨工艺生产得到。棕榈仁粕价格低廉，无致毒、致敏抗营养因子，约含10％残油、15％蛋白质，可少量添加在畜禽饲料中。但是，低蛋白、高粗纤维、适口性差等因素制约棕榈仁粕成为优质的饲料原料。此外棕榈仁粕市场价不到1500元每吨，商业价值较低。
我国饲料产量全球第一，但优质蛋白资源严重短缺，大豆等主要饲料蛋白原料对外依存度高达85％以上。因此，开发棕榈等新型非粮蛋白源以保证我国粮食安全和经济安全变得刻不容缓。
发明内容
本发明的目的在于提供一种棕榈仁浓缩蛋白、其制备方法及生产系统，通过棕榈仁浓缩蛋白方法的设计以解决现有技术中即通过机械压榨或溶剂萃取法获得的棕榈仁粕粗纤维含量高，蛋白含量低、商业价值较低等问题。
本发明提供了一种制备棕榈仁浓缩蛋白的方法，包括如下制备步骤：
棕榈仁仁壳分离，获得去除坚硬外壳的棕榈果仁；
将去除坚硬外壳的棕榈果仁浸泡水中，软化后在低温下经轧胚工艺获得一定厚度的胚片，压榨出一部分油脂；
低温下对胚片进行烘干，烘干后的胚片浸泡在有机溶剂中进行脱脂提油处理，脱脂提油后，获得待脱糖胚片；
将待脱糖胚片进行超声处理，超声处理后，将待脱糖胚片浸泡于乙醇溶剂中，进行脱糖处理，浸泡一定时间后，获得脱糖的胚片；
将脱糖胚片经挤压工艺去除残留的乙醇溶剂后低温烘干，获得棕榈仁浓缩蛋白。
优选地，待脱糖胚片浸泡于水中，进行脱糖处理，浸泡时间为0.5-3h。
优选地，轧胚工艺在50-70℃下进行。
优选地，胚片的烘干温度为50-70℃。
优选地，胚片的厚度为0.3-1.0mm。
优选地，烘干后的胚片浸泡在有机溶剂的时间为2-5h。
优选地，有机溶剂为正己烷、石油醚、正戊烷、正庚烷、环己烷中的至少一种。
优选地，获得的棕榈仁浓缩蛋白中蛋白的质量含量≥40％。
优选地，乙醇的浓度为75％。
本发明还提供了一种基于如上述中任一项所述的制备棕榈仁浓缩蛋白的方法获得的棕榈仁浓缩蛋白。
本发明还提供了一种基于如上述所述的棕榈仁浓缩蛋白的生产系统，
包括棕榈仁仁壳分离机、扎胚机、脱脂提纯机、超声波处理器、脱糖机和烘干机。
本发明提供的一种棕榈仁浓缩蛋白、其制备方法及生产系统与现有技术相比具有以下进步：
1、本发明提出的制备棕榈仁浓缩蛋白的方法，可以获得棕榈仁浓缩蛋白，较传统的棕榈仁粕的蛋白含量提高了近一倍，脂肪含量经过溶剂萃取脱脂，由原来的9％降低到1％；必需的氨基酸含量均较传统棕榈仁粕均有很大幅度提高，总氨基酸含量提高了一倍以上，可以作为主要动物性饲料源豆粕的替代品。
2、本发明提供的制备棕榈仁浓缩蛋白的方法，获得的棕榈仁浓缩蛋白，其精氨酸和蛋氨酸含量与豆粕相近，鸡对经过处理获得的新型棕榈仁浓缩蛋白氨基酸酸利用率均略有提高，其中精氨酸、组氨酸、赖氨酸、亮氨酸、蛋氨酸和苯丙氨酸的利用率均高于90％。
下面将结合对本发明的技术方案进行清楚、完整地描述，显然，所描述的实施例是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
发明提供的一种制备棕榈仁浓缩蛋白的方法，包括如下制备步骤：
S1)棕榈仁仁壳分离，获得去除坚硬外壳的棕榈果仁；
S2)将去除坚硬外壳的棕榈果仁浸泡水中，软化后在低温下经轧胚工艺获得一定厚度的胚片，压榨出一部分油脂；
S3)低温下对胚片进行烘干，烘干后的胚片浸泡在有机溶剂中进行脱脂提油处理，脱脂提油后，获得待脱糖胚片；
S4)将待脱糖胚片先进行超声处理，超声处理后，将待脱糖胚片浸泡于乙醇溶剂中，进行脱糖处理，浸泡一定时间后，获得脱糖的胚片；
S5)将脱糖胚片经挤压工艺去除残留的乙醇溶剂后低温烘干，获得棕榈仁浓缩蛋白。
具体地，待脱糖胚片浸泡于水中，进行脱糖处理，浸泡时间为0.5-3h。
具体地，轧胚工艺在50-70℃下进行。
具体地，胚片的烘干温度为50-70℃。
具体地，胚片的厚度为0.3-1.0mm。
具体地，烘干后的胚片浸泡在有机溶剂的时间为2-5h。
具体地，有机溶剂为正己烷、石油醚、正戊烷、正庚烷、环己烷中的至少一种；
将待脱糖胚片浸泡于溶剂中，浸泡的溶剂为乙醇。
具体地，获得的棕榈仁浓缩蛋白中蛋白的质量含量≥40％。
具体地，乙醇的浓度为75％。
实施例一
制备棕榈仁浓缩蛋白的方法，包括如下制备步骤：
101)采用剥壳机实现棕榈仁仁壳分离，获得去除坚硬外壳的棕榈果仁；
102)将去除坚硬外科的棕榈果仁在水中浸泡24h后，软化后，在60℃下经轧胚工艺获得胚片，胚片的厚度为0.5mm；压榨出一部分油脂；
103)在60℃下，对胚片进行烘干，烘干后的胚片浸泡在正己烷溶剂中进行脱脂提油，浸泡5h后，获得待脱糖胚片；
104)将待脱糖胚片进行超声处理，超声处理30min，将待脱糖胚片浸泡于乙醇溶剂中，乙醇的浓度为75％，进行脱糖处理，浸泡3h后，获得脱糖的胚片；
105)将脱糖胚片经挤压工艺去除残留的乙醇后低温烘干，获得棕榈仁浓缩蛋白。
其中，轧胚工艺为采用轧胚机对软化的棕榈果仁进行轧胚。
其中，超声处理过程为，将待脱糖胚片放入容器中，采用超声波处理器对待脱糖胚片进行超声处理。
分别采用凯氏定氮法(GB/T 6432—1994)、酸水解粗脂肪测定法(GB/T 6433—2006)、550℃灼烧法(GB/T 6438—2007)分别测定样品的粗蛋白质、粗脂肪和粗灰分含量；氨基酸含量采用国标GB/T 18246—2000方法进行测定。获得的棕榈仁浓缩蛋白成分见表1。
鸡对棕榈仁浓缩蛋白氨基酸酸利用率采用真代谢能法(Sibbald,1987)，试验鸡禁食24h后，收集48h内源排泄物；然后每个处理的每只鸡，用强饲器一次投饲40g各自日粮，随后开始计时并收集48h排泄物。饲料样品的收集、取样、分析等与常规方法相同，排泄物放至4℃温度保存。每个样品至少测定三个重复，鸡对棕榈仁浓缩蛋白氨基酸酸利用率见表2。
本实施例通过剥壳机进行仁壳分离以去除硬壳，并通过精分工序最大程度去除木质素纤维成分，保证后续加工过程中产品的蛋白质含量。
本实施例还提供了一种棕榈仁浓缩蛋白的生产系统，
包括棕榈仁仁壳分离机、扎胚机、脱脂提纯机、超声波处理器、脱糖机和烘干机。
其中，棕榈仁仁壳分离机采用河南国瑞油脂工程技术有限公司生产的棕榈仁壳分离机。
扎胚机采用山东邦腾机械设备有限公司生产的扎胚机。
脱脂提纯机采用巩义市源隆机械有限公司生产的脱脂提纯机。
超声波处理器采用方需科技(上海)有限公司生产的非接触式超声波破碎仪。
脱糖机采用新疆金兰植物蛋白有限公司生产的脱糖机。
烘干机采用郑州泰达节能干燥设备有限公司定制的烘干机。
实施例二
本实施例是在实施例一的基础进一步改进，实施例一公开的内容，此处不再赘述。
制备棕榈仁浓缩蛋白的方法，包括如下制备步骤：
201)采用剥壳机实现棕榈仁仁壳分离，获得去除坚硬外壳的棕榈果仁；
202)将去除坚硬外壳的棕榈果仁浸泡于水中10h，软化后，在50℃下经轧胚工艺获得一定厚度的胚片；压榨出一部分油脂；
203)在50℃下，对胚片进行烘干，烘干后的胚片浸泡在有机溶剂中进行脱脂提油，浸泡2h,脱油后，获得待脱糖胚片；
204)将待脱糖胚片进行超声处理，超声30min处理后，将待脱糖胚片浸泡于75％乙醇溶剂中，进行脱糖处理，浸泡3h后，获得脱糖的胚片；
205)将脱糖胚片经挤压工艺去除残留的乙醇后低温烘干，获得棕榈仁浓缩蛋白。
本实施例的胚片的厚度为0.3mm；
本实施例的有机溶剂为石油醚；
本实施例的获得的棕榈仁浓缩蛋白成分和榈仁浓缩蛋白氨基酸利用率见表1和表2。
实施例三
本实施例是在实施例一的基础进一步改进，实施例一公开的内容，此处不再赘述。
制备棕榈仁浓缩蛋白的方法，包括如下制备步骤：
301)采用剥壳机实现棕榈仁仁壳分离，获得去除坚硬外壳的棕榈果仁；
302)将去除坚硬外壳的棕榈果仁浸泡于水中17h，软化后，在70℃下经轧胚工艺获得一定厚度的胚片，压榨出一部分油脂；
303)在70℃下，对胚片进行烘干，烘干后的胚片浸泡在有机溶剂中进行脱脂提油，浸泡4h,脱油后，获得待脱糖胚片；
304)将待脱糖胚片进行超声处理，超声30min处理后，将待脱糖胚片浸泡于75％乙醇溶剂中，进行脱糖处理，浸泡3h后，获得脱糖的胚片；
305)将脱糖胚片经挤压工艺去除残留的乙醇后低温烘干，获得棕榈仁浓缩蛋白。
本实施例的胚片的厚度为1.0mm；
本实施例的有机溶剂为正戊烷。
本实施例的获得的棕榈仁浓缩蛋白成分和榈仁浓缩蛋白氨基酸利用率见表1和表2。
对比例1选取棕榈仁粕(从天津津悦农产品有限公司采购)。
对比例2选取去皮豆粕(从青岛渤海科技有限责任公司采购)。
从表1和表2中可以看到，实施例一、实施例二和实施例三的棕榈仁浓缩蛋白的蛋白含量超过41％，相比传统的棕榈仁粕(蛋白含量为16％左右)提高了近一倍。脂肪含量经过溶剂萃取脱脂，由原来的9％降低到1％。必需的氨基酸含量均较传统棕榈仁粕均有很大幅度提高，总氨基酸含量提高了一倍以上。
棕榈仁粕精氨酸和蛋氨酸含量较高，而获得的新型棕榈仁浓缩蛋白经过大幅度的提高后，其精氨酸和蛋氨酸含量与豆粕相近，但其他氨基酸水平仍然低于豆粕。实施例一的棕榈仁浓缩蛋白的氨基酸含量占粗蛋白的88％，与豆粕类似，此比例远远高于对比例1的棕榈仁粕(83％)。鸡对经过处理获得的棕榈仁浓缩蛋白的氨基酸酸利用率相比棕榈粕均略有提高，其中精氨酸、组氨酸、赖氨酸、亮氨酸、蛋氨酸和苯丙氨酸的利用率均高于90％。
实施例四
本实施例棕榈仁浓缩蛋白与实施例一的制备过程相同，区别仅在于将待脱糖胚片浸泡于乙醇溶剂中，乙醇浓度为75％，进行脱糖处理，浸泡时间为0.5h，脱糖的胚片中非淀粉多糖含量见表3。
实施例五
本实施例棕榈仁浓缩蛋白与实施例一的制备过程相同，区别仅在于将待脱糖胚片浸泡于乙醇溶剂中，乙醇浓度为75％，进行脱糖处理，浸泡时间为1h，脱糖的胚片中非淀粉多糖含量见表3。
实施例六
本实施例棕榈仁浓缩蛋白与实施例一的制备过程相同，区别仅在于将待脱糖胚片浸泡于乙醇溶剂中，乙醇浓度为75％，进行脱糖处理，浸泡时间为2h，脱糖的胚片中非淀粉多糖含量见表3。
实施例七
本实施例棕榈仁浓缩蛋白与实施例一的制备过程相同，区别仅在于将待脱糖胚片浸泡于乙醇溶剂中，乙醇浓度为75％，进行脱糖处理，浸泡时间为3h，脱糖的胚片中非淀粉多糖含量见表3。
实施例四、实施例五、实施例六和实施例七相比实施例一，仅是改变了脱糖处理过程中待脱糖胚片浸泡于乙醇的时间，可以实现对可溶性非淀粉多糖的提取率的控制，可以根据不同的产品需求控制棕榈仁浓缩蛋白产品中的可溶性非淀粉多糖含量，从而获得不同系列的棕榈仁浓缩蛋白产品。
表1：棕榈仁浓缩蛋白、棕榈仁粕和豆粕的必需氨基酸组成(％)
表2：鸡对棕榈仁浓缩蛋白氨基酸酸利用率(％)
表3：不同浸泡时间脱糖后胚片的非淀粉多糖含量(％)
最后应说明的是：以上各实施例仅用以说明本发明的技术方案，而非对其限制；尽管参照前述各实施例对本发明进行了详细的说明，本领域的普通技术人员应当理解：其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分或者全部技术特征进行等同替换；而这些修改或者替换，并不使相应技术方案的本质脱离本发明各实施例技术方案的范围。
Claims (10)
- 一种制备棕榈仁浓缩蛋白的方法，其特征在于：包括如下制备步骤：棕榈仁仁壳分离，获得去除坚硬外壳的棕榈果仁；将去除坚硬外壳的棕榈果仁浸泡水中，软化后在低温下经轧胚工艺获得一定厚度的胚片，压榨出一部分油脂；低温下对胚片进行烘干，烘干后的胚片浸泡在有机溶剂中进行脱脂提油处理，脱脂提油后，获得待脱糖胚片；将待脱糖胚片进行超声处理，超声处理后，将待脱糖胚片浸泡于乙醇溶剂中，进行脱糖处理，浸泡一定时间后，获得脱糖后的胚片；将脱糖胚片经挤压工艺去除残留的乙醇溶剂后低温烘干，获得棕榈仁浓缩蛋白。
- 根据权利要求1所述的制备棕榈仁浓缩蛋白的方法，其特征在于：待脱糖胚片浸泡于水中，进行脱糖处理，浸泡时间为0.5-3h。
- 根据权利要求1所述的制备棕榈仁浓缩蛋白的方法，其特征在于：轧胚工艺在50-70℃下进行。
- 根据权利要求1所述的制备棕榈仁浓缩蛋白的方法，其特征在于：胚片的烘干温度为50-70℃。
- 根据权利要求1所述的制备棕榈仁浓缩蛋白的方法，其特征在于：胚片的厚度为0.3-1.0mm。
- 根据权利要求1所述的制备棕榈仁浓缩蛋白的方法，其特征在于：烘干后的胚片浸泡在有机溶剂的时间为2-5h。
- 根据权利要求1所述的制备棕榈仁浓缩蛋白的方法，其特征在于：有机溶剂为正己烷、石油醚、正戊烷、正庚烷、环己烷中的至少一种。
- 根据权利要求1所述的制备棕榈仁浓缩蛋白的方法，其特征在于：获得的棕榈仁浓缩蛋白中蛋白的质量含量≥40％；乙醇的浓度为75％。
- 一种基于如权利要求1-8中任一项所述的制备棕榈仁浓缩蛋白的方法获得的棕榈仁浓缩蛋白。
- 一种基于如权利要求9所述的棕榈仁浓缩蛋白的生产系统，其特征在于：包括棕榈仁仁壳分离机、扎胚机、脱脂提纯机、超声波处理器、脱糖机和烘干机。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210681152.7A CN115005317A (zh) | 2022-06-16 | 2022-06-16 | 一种棕榈仁浓缩蛋白、其制备方法及生产系统 |
| CN202210681152.7 | 2022-06-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2023241315A1 true WO2023241315A1 (zh) | 2023-12-21 |
Family
ID=83074042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CN2023/095626 WO2023241315A1 (zh) | 2022-06-16 | 2023-05-22 | 一种棕榈仁浓缩蛋白、其制备方法及生产系统 |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN115005317A (zh) |
| WO (1) | WO2023241315A1 (zh) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115005317A (zh) * | 2022-06-16 | 2022-09-06 | 中国农业科学院饲料研究所 | 一种棕榈仁浓缩蛋白、其制备方法及生产系统 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090317512A1 (en) * | 2008-06-20 | 2009-12-24 | N.V. De Smet Ballestra Engineering S.A. | Vegetable protein concentrate |
| CN101845077A (zh) * | 2010-05-17 | 2010-09-29 | 天津实发中科百奥工业生物技术有限公司 | 超声连续逆流浸提醇法大豆浓缩蛋白的制备工艺 |
| CN101851268A (zh) * | 2010-04-30 | 2010-10-06 | 祁鲲 | 湿豆粕直接制取大豆浓缩蛋白工艺 |
| CN104872394A (zh) * | 2015-06-05 | 2015-09-02 | 黄石市佳农饲料有限公司 | 一种去除抗营养因子的棕榈仁粕及其制备方法和应用 |
| CN108271916A (zh) * | 2018-01-31 | 2018-07-13 | 河南华泰粮油机械股份有限公司 | 一种棉籽浓缩蛋白的提取方法 |
| CN109566849A (zh) * | 2019-01-21 | 2019-04-05 | 福建长德蛋白科技有限公司 | 一种大豆浓缩蛋白的提取新方法 |
| CN115005317A (zh) * | 2022-06-16 | 2022-09-06 | 中国农业科学院饲料研究所 | 一种棕榈仁浓缩蛋白、其制备方法及生产系统 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102038113B (zh) * | 2010-12-28 | 2013-01-09 | 通威股份有限公司 | 一种含有棕榈仁粕的鸭饲料及其制备方法 |
| CN107410663A (zh) * | 2017-04-28 | 2017-12-01 | 济南中棉生物科技有限公司 | 一种进一步脱除脂类物质的大豆粕生产浓缩蛋白的方法 |
-
2022
- 2022-06-16 CN CN202210681152.7A patent/CN115005317A/zh active Pending
-
2023
- 2023-05-22 WO PCT/CN2023/095626 patent/WO2023241315A1/zh unknown
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090317512A1 (en) * | 2008-06-20 | 2009-12-24 | N.V. De Smet Ballestra Engineering S.A. | Vegetable protein concentrate |
| CN101851268A (zh) * | 2010-04-30 | 2010-10-06 | 祁鲲 | 湿豆粕直接制取大豆浓缩蛋白工艺 |
| CN101845077A (zh) * | 2010-05-17 | 2010-09-29 | 天津实发中科百奥工业生物技术有限公司 | 超声连续逆流浸提醇法大豆浓缩蛋白的制备工艺 |
| CN104872394A (zh) * | 2015-06-05 | 2015-09-02 | 黄石市佳农饲料有限公司 | 一种去除抗营养因子的棕榈仁粕及其制备方法和应用 |
| CN108271916A (zh) * | 2018-01-31 | 2018-07-13 | 河南华泰粮油机械股份有限公司 | 一种棉籽浓缩蛋白的提取方法 |
| CN109566849A (zh) * | 2019-01-21 | 2019-04-05 | 福建长德蛋白科技有限公司 | 一种大豆浓缩蛋白的提取新方法 |
| CN115005317A (zh) * | 2022-06-16 | 2022-09-06 | 中国农业科学院饲料研究所 | 一种棕榈仁浓缩蛋白、其制备方法及生产系统 |
Non-Patent Citations (1)
| Title |
|---|
| LIU XIAOGENG: "The Resources of Xylophyta Oil Cakes ＆ Meals and its Development and Utilization", JOURNAL OF CEREALS ＆ OILS., no. 4, 1 January 2003 (2003-01-01), pages 10 - 13, XP093117282 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115005317A (zh) | 2022-09-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101785527B (zh) | 一种脱酚棉籽蛋白和棉籽糖的制取方法 | |
| WO2023241315A1 (zh) | 一种棕榈仁浓缩蛋白、其制备方法及生产系统 | |
| CN110699412B (zh) | 一种从富硒百香果籽提取硒多肽的方法 | |
| CN103387599A (zh) | 一种桑叶蛋白的提取工艺 | |
| AU2003252766A1 (en) | Integrated process for separation of oil, protein, carbohydrates, shell and minor toxic components from seeds | |
| CN103396878A (zh) | 一种低温冷榨花生油的制备方法 | |
| CN113444567A (zh) | 脱酚浓缩棉籽蛋白萃取工艺 | |
| CN102363626A (zh) | 一种油茶饼储藏蛋白质的制备方法 | |
| CN111019756A (zh) | 一种油茶籽的工业化生产加工方法 | |
| CN107043649B (zh) | 近固态酶解联合物理压榨制取油茶籽油的方法 | |
| CN109566849A (zh) | 一种大豆浓缩蛋白的提取新方法 | |
| CN101575369A (zh) | 低温冷榨菜籽粕膜分离制备菜籽蛋白联产菜籽多糖的工艺方法 | |
| CN104045685A (zh) | 一种用油茶籽饼提取蛋白质的方法 | |
| CN105054110A (zh) | 一种动物骨头的加工方法 | |
| CN102349595A (zh) | 一种提高棉籽蛋白的吸收利用率的工业方法 | |
| CN1125875C (zh) | 棉籽油料萃取脱毒方法 | |
| CN102450387A (zh) | 加工鱼粉的方法 | |
| CN102450384A (zh) | 一种从大豆油渣提取大豆蛋白的方法 | |
| CN107094985B (zh) | 一种用花生粕联产花生浓缩蛋白和副产品的生产方法 | |
| CN110643052A (zh) | 一种氢氧化钠/尿素水溶液处理杜仲果壳提取杜仲胶的方法 | |
| CN102219781A (zh) | 在水飞蓟种皮中提取水飞蓟素的方法 | |
| CN103652308A (zh) | 高蛋白葡萄籽粕工业化制取方法 | |
| CN113412958B (zh) | 一种造纸法再造烟叶水膏的提质方法、烟叶水膏及用途 | |
| CN112219933B (zh) | 一种脱酚棉籽蛋白的提取工艺及其用途 | |
| CN204907859U (zh) | 一种紫苏籽清洗分拣设备 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 23822885 Country of ref document: EP Kind code of ref document: A1 |