WO2022210204A1 - 粉砕方法、高分子ブロック製造方法及び粉砕装置 - Google Patents

粉砕方法、高分子ブロック製造方法及び粉砕装置 Download PDF

Info

Publication number
WO2022210204A1
WO2022210204A1 PCT/JP2022/013691 JP2022013691W WO2022210204A1 WO 2022210204 A1 WO2022210204 A1 WO 2022210204A1 JP 2022013691 W JP2022013691 W JP 2022013691W WO 2022210204 A1 WO2022210204 A1 WO 2022210204A1
Authority
WO
WIPO (PCT)
Prior art keywords
pulverizing
porous polymer
pulverization
amino acid
section
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2022/013691
Other languages
English (en)
French (fr)
Japanese (ja)
Inventor
和人 福永
伸彦 加藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Corp
Original Assignee
Fujifilm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujifilm Corp filed Critical Fujifilm Corp
Priority to JP2023511086A priority Critical patent/JPWO2022210204A1/ja
Priority to EP22780418.4A priority patent/EP4299182A4/en
Priority to CN202280023461.3A priority patent/CN117042884A/zh
Publication of WO2022210204A1 publication Critical patent/WO2022210204A1/ja
Priority to US18/477,630 priority patent/US20240017267A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
    • B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
    • B02C18/08—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers
    • B02C18/086—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers specially adapted for disintegrating plastics, e.g. cinematographic films
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C19/00—Other disintegrating devices or methods
    • B02C19/18—Use of auxiliary physical effects, e.g. ultrasonic waves or irradiation, for disintegrating
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00—Processes of treating or compounding macromolecular substances
    • C08J3/12—Powdering or granulating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C19/00—Other disintegrating devices or methods
    • B02C19/06—Jet mills

Definitions

  • the present disclosure relates to a pulverizing method, a polymer block manufacturing method, and a pulverizing apparatus.
  • Regenerative medicine is a medical technology that uses the three factors of cells, scaffolds, and growth factors to recreate the same form and function as the original tissue in living tissue that cannot be restored by the body's natural healing ability alone. be.
  • bone regeneration in the field of orthopedics or dentistry is known as one of the fields that are attracting attention in the field of regenerative medicine.
  • QOL Quality of Life
  • Japanese Patent Application Laid-Open No. 55-092667 discloses a method of pulverizing at an ultra-low temperature using liquid nitrogen.
  • Japanese Patent Application Laid-Open No. 9-000176 discloses a method of wet pulverizing by mixing with a liquid.
  • the present disclosure provides a pulverizing method, a polymer block manufacturing method, and a pulverizing apparatus that can suppress scorching that occurs when pulverizing a polymeric porous body.
  • a first aspect of the present disclosure is a pulverization method, which includes a pulverization step of pulverizing a porous polymer body and a static elimination step of eliminating static electricity from the porous polymer body being pulverized in the pulverization step.
  • static electricity may be removed from the porous polymer body by irradiating the porous polymer body with radiation.
  • the radiation may be at least one of X-rays and ultraviolet rays.
  • the radiation may be X-rays having a radiation source with a tube voltage of 4 kV or more and 50 kV or less.
  • a fifth aspect of the present disclosure is that, in the static elimination step according to the second to fourth aspects, the 70 ⁇ m dose equivalent rate of the radiation irradiated to the porous polymer body is 1 mSv/h or more and 200 Sv/h or less. may be
  • a sixth aspect of the present disclosure is that, in the static elimination step according to the above aspect, the static elimination time required for the potential to change from +1000 V to +100 V in at least a part of the pulverizing unit that pulverizes the porous polymer body is 0.01 second or more. , 10 seconds or less.
  • the porous polymer body may be pulverized using a pulverizing unit that performs dry pulverization.
  • the crushing section may include a screen and a rotating impeller.
  • a ninth aspect of the present disclosure is the seventh aspect or the eighth aspect, wherein the crushing unit is ventilated at a ventilation frequency of 1 ⁇ 10 4 times/h or more and 1 ⁇ 10 7 times/h or less. good.
  • static electricity may be eliminated from the porous polymer body by irradiating at least part of the pulverized portion with radiation.
  • the porous polymer body may contain protein.
  • the porous polymer body may contain the following peptide (A), (B) or (C).
  • A A peptide consisting of the amino acid sequence of SEQ ID NO:1.
  • B A peptide consisting of an amino acid sequence in which one or several amino acid residues are modified in the amino acid sequence of SEQ ID NO: 1 and having biocompatibility.
  • C consisting of an amino acid sequence having a partial sequence having 80% or more sequence identity with the partial amino acid sequence consisting of the 4th to 192nd amino acid residues in the amino acid sequence of SEQ ID NO: 1, and biocompatibility Peptides with
  • the size of the porous polymer body before pulverization may be 0.1 mm or more and 50 mm or less.
  • the size of the pulverized porous polymer body may be 0.01 mm or more and 10 mm or less.
  • a fifteenth aspect of the present disclosure is a method for producing a polymer block, comprising: a pulverization step of pulverizing a porous polymer body; .
  • a sixteenth aspect of the present disclosure is a pulverizing apparatus comprising: a pulverizing unit for pulverizing a porous polymer body; and an irradiation unit for irradiating at least part of the pulverizing unit with radiation.
  • the porous polymer material being pulverized in the pulverizing section is neutralized by the radiation emitted from the pulverizing section.
  • the crushing device further includes a ventilation part for ventilating the crushing part, and the ventilation frequency of the crushing part by the ventilation part is 1 ⁇ 10 4 times/h or more, It may be 1 ⁇ 10 7 times/h or less.
  • step is used not only for independent steps, but also for cases where the intended effect of this step is achieved even if it cannot be clearly distinguished from other steps. included.
  • the pulverization method, the polymer block production method, and the pulverization apparatus of the present disclosure can suppress scorching that occurs when pulverizing the porous polymer body.
  • a method for pulverizing a porous polymer material includes a pulverizing step of pulverizing the porous polymer material and a static elimination step of eliminating static electricity from the porous polymer material being pulverized in the pulverizing process.
  • a pulverizing step of pulverizing the porous polymer material includes a static elimination step of eliminating static electricity from the porous polymer material being pulverized in the pulverizing process.
  • the polymer porous body after pulverization in the pulverization step is referred to as "polymer block”.
  • the method according to the present exemplary embodiment performs a pulverization step of pulverizing the porous polymer material and a static elimination step of removing static electricity from the porous polymer material being pulverized in the pulverization process.
  • the pulverization method and the polymer block manufacturing method according to the present exemplary embodiment will be collectively referred to as the “method of the present disclosure”.
  • the pulverization step and the static elimination step may be repeated again.
  • the number of times the pulverization step and the static elimination step are performed is preferably 1 to 4 times, more preferably 2 to 3 times.
  • the method of the present disclosure includes a pulverization step of pulverizing the porous polymeric body.
  • pulverization is to break up solids and aggregates of solids by applying mechanical energy.
  • pulverization means in the method of the present disclosure include dry pulverization and wet pulverization, but dry pulverization is preferably used from the viewpoint of saving the labor and cost required for drying and the like for separating and removing the liquid.
  • the pulverization can be performed by a pulverizing section (details will be described later) provided in the pulverizing device.
  • the method of the present disclosure includes a charge removal step of removing charges from the porous polymer material being crushed in the crushing step.
  • static elimination means removing static electricity from a charged substance. Examples of static elimination methods in the method of the present disclosure include a radiation irradiation method, a corona discharge method, an antistatic agent addition method, a humidification method, and the like. Moreover, you may combine two or more of these static elimination methods suitably.
  • the radiation irradiation method and the corona discharge method are preferable from the viewpoint of not requiring the addition of an antistatic agent or the like and the formation of a high-humidity environment that may affect the friction coefficient of the material to be pulverized.
  • the radiation irradiation method it is possible to enhance the static elimination ability. Irradiation of radiation can be performed by an irradiation unit (details will be described later) provided in the pulverization device.
  • the "radiation" irradiated to the porous polymer material in the static elimination process includes particle beams such as alpha rays and beta rays, electromagnetic waves such as X rays and gamma rays, and non-ionizing radiation such as infrared rays, visible rays and ultraviolet rays.
  • the radiation is preferably at least one of X-rays and ultraviolet rays from the viewpoint that the porous polymer material to be irradiated is unlikely to be denatured.
  • an X-ray source with a tube voltage of 4 kV or more and 50 kV or less (so-called soft X-ray). X-rays of 20 kV or less are particularly preferred.
  • the 70 ⁇ m dose equivalent rate of the radiation is preferably 1 mSv/h or more and 200 Sv/h or less, more preferably 2 mSv/h or more and 200 Sv/h or less.
  • the exposure dose of radiation can be measured by, for example, a survey meter.
  • the 70 ⁇ m dose equivalent rate of radiation is a value measured based on the method described in (Measurement of irradiation dose) in Example 1 below. In the static elimination step, it is preferable that the radiation continues to be irradiated during the pulverization of the porous polymer body in the pulverization step.
  • the static elimination ability can be evaluated using a charge plate monitor (also called a charge plate monitor, an ionizer performance tester, etc.).
  • a charge plate monitor also called a charge plate monitor, an ionizer performance tester, etc.
  • an ANSI/ESD-STM 3.1 compliant charge plate monitor such as MODEL 156A (manufactured by Trek) or the like can be used.
  • the static elimination time In a charged electrode having a specific electrostatic capacity, the time required to change from a specific initial potential to a specific potential due to static elimination is called the static elimination time. By measuring this, the time required for static elimination can be evaluated. can.
  • the time until the initial potential of +1000 V changes to a potential of +100 V is measured as the neutralization time.
  • the static elimination time measured by this method is preferably 0.01 seconds or more and 10 seconds or less, more preferably 0.01 seconds or more and 2 seconds or less, in at least a part of the grinding section of the grinding device. .
  • the method of the present disclosure may further include a classification step of classifying the polymer block (porous polymer body after pulverization).
  • “Classification” is an operation of separating polymer blocks having different properties according to their properties. Properties of the polymer block include size, shape, density, magnetism, color and chemical composition. Examples of the method for classifying polymer blocks according to the size thereof include fluid classification performed by interposing a fluid, and sieving to separate polymer blocks larger than the mesh of the sieve and polymer blocks smaller than the mesh by using a sieve. . A cyclone may be applied as the fluid classification. Sieves, meshes, filters and the like may be applied for sieving.
  • the classification step may be performed simultaneously with the pulverization step and/or the static elimination step, or may be performed after the pulverization step and/or the static elimination step.
  • the classification step by sieving can be performed using a sieve for classification provided in the pulverizer (details will be described later).
  • the porous polymer material during pulverization may be charged and attached to the pulverization section due to static electricity generated by friction with the pulverization section and/or other porous polymer bodies. If the porous polymer material during pulverization is electrically charged and adheres to the pulverized portion, the friction time increases and the amount of frictional heat also increases, making scorching more likely to occur.
  • the term "friction time” as used herein refers to the time during which the porous polymer material during pulverization is subjected to frictional force due to friction with the pulverized portion and/or other porous polymer material.
  • Electrostatic adhesion is determined by the difference between the force of adhesion due to electrostatic force, etc., and the force of desorption due to gravity, etc., so the lower the density of an object, the smaller the detachment force compared to the adhesion force, and the easier it is for electrostatic adhesion to occur. Therefore, a porous polymer material having a lower density than a non-porous polymer material tends to be easily charged and adhered to the pulverized portion, and tends to be easily scorched.
  • the term "charring” refers to a change in properties (for example, coloration) due to oxidation, decomposition, cross-linking and/or Maillard reaction in the porous polymer due to temperature rise due to frictional heat.
  • FIG. 1 is a schematic diagram showing the configuration of the crushing device 10. As shown in FIG.
  • the pulverizing device 10 has the function of performing the pulverizing step, the static elimination step and the classifying step in the method according to the above exemplary embodiment.
  • the crushing device 10 includes a crushing unit 20, at least one irradiation unit 30, and a ventilation unit 40.
  • FIG. 1 shows three irradiating units 30A to 30C as an example of the irradiating unit 30, but hereinafter, the irradiating units 30A to 30C are simply referred to as “irradiating units 30” when not distinguished.
  • the pulverizing device 10 when the porous polymer material is introduced from the charging section 12 , the porous polymer material is pulverized by the pulverizing section 20 and discharged from the discharging section 14 .
  • Each arrow A in FIG. 1 indicates the flow of air ventilated by the ventilation section 40, and the porous polymer material is also transported along this flow.
  • the pulverizing section 20 pulverizes the porous polymer body.
  • a pulverizing function included in various devices for performing known dry pulverization and wet pulverization can be applied.
  • Such equipment includes screen mills, hammer mills, ball mills, bead mills, jet mills, cutter mills, vibrating mills, roller mills, line mills, stamp mills, disc mills, pin mills, grinding mills, rotor mills, cyclone mills, rod mills. , power mills, pot mills, jaw crushers, gyratory crushers, impact crushers, roll crushers and edge runners.
  • FIG. 1 shows a diagram using a grinding function included in a screen mill as a grinding section 20 .
  • the screen mill includes a multi-perforated screen 22 and a rotating impeller 24 as grinding functions.
  • the porous polymer material charged from the charging unit 12 is pressed against the screen 22 using the rotating impeller 24 so that the porous polymer material is reduced to the size of the pores of the screen 22 or less.
  • the rotating impeller is preferably rake-shaped, round or square, more preferably rake-shaped. It should be noted that FIG. 1 omits illustration of a motor for driving the rotating impeller 24 and the like.
  • the hole diameter of the screen 22 is preferably 0.006 inch or more and 0.25 inch or less, and is preferably 0.04 inch or more and 0.04 inch or more. 08 inches or less is more preferable.
  • the rotational speed of the rotary impeller is preferably 100 rpm or more and 6000 rpm or less, more preferably 1000 rpm or more and 3000 rpm or less.
  • the wind speed at the holes of the screen 22 is preferably 5 m/s or more and 20 m/s or less, more preferably 7 m/s or more and 15 m/s or less.
  • the pulverizing section 20 refers to a region where the porous polymer body can actually be pulverized.
  • the screen 22 and the rotary impeller 24 provided in the screen mill correspond to the crushing section 20
  • other components provided in the screen mill do not correspond to the crushing section 20 .
  • the input section and the discharge section provided in a known screen mill correspond to the input section 12 and the discharge section 14 of the pulverizer 10, respectively.
  • the irradiation unit 30 irradiates at least part of the pulverizing unit 20 with radiation to irradiate the porous polymer material being pulverized in the pulverizing unit 20 with radiation, thereby neutralizing the porous polymer material.
  • the air in the pulverizing section 20 is ionized to generate positive and negative ions. That is, ions having charges opposite to those of the porous polymer material charged by static electricity are generated. As a result, the charge of the porous polymer body is neutralized, and the charge of the porous polymer body is eliminated. Since the porous polymer body is agitated in the pulverizing section 20, if the porous polymer body is neutralized in at least a part of the pulverizing section 20, the effect of suppressing the occurrence of charring can be obtained.
  • the irradiation unit 30 may irradiate the input unit 12 and the discharge unit 14 in addition to at least a part of the crushing unit 20 with radiation.
  • the irradiation unit 30 may irradiate the input unit 12 and the discharge unit 14 in addition to at least a part of the crushing unit 20 with radiation.
  • the position of the irradiation units 30 in the pulverization device 10 is not particularly limited as long as at least one of the irradiation units 30 can irradiate at least part of the pulverization unit 20 with radiation.
  • at least one of the irradiation units 30 is preferably provided at an angle of 30 to 90 degrees, more preferably at an angle of 45 to 90 degrees, with respect to the vertical direction of the crushing device 10 .
  • another one of the irradiation units 30 is preferably provided at an angle of 0 to 60 degrees, more preferably at an angle of 0 to 45 degrees, with respect to the vertical direction of the crushing device 10 .
  • the pulverizing device 10 may include a sieve 16 for classifying the polymer blocks pulverized by the pulverizing section 20.
  • a sieve 16 for classifying the polymer blocks pulverized by the pulverizing section 20.
  • the crushing device 10 may perform a ventilation step of ventilating the inside of the crushing unit 20.
  • the ventilating section 40 may ventilate the inside of the pulverizing section 20 by sucking the air inside the pulverizing device 10 as indicated by arrows A in FIG.
  • the ventilation unit 40 By ventilating the inside of the pulverizing unit 20 with the ventilation unit 40, the air in the vicinity of the porous polymer material being pulverized can be exchanged. can be efficiently ionized by the radiation emitted by Therefore, the static elimination effect can be improved.
  • the ventilation frequency of the pulverizing section 20 by the ventilation section 40 is preferably 1 ⁇ 10 4 times/h or more and 1 ⁇ 10 7 times/h or less. Further, the ventilation frequency of the crushing unit 20 by the ventilation unit 40 is more preferably 1 ⁇ 10 5 times/h or more and 1 ⁇ 10 7 times/h or less, and more preferably 2.5 ⁇ 10 5 times/h or more, 1 It is more preferable that it is x10 7 times/h or less.
  • the volume of the pulverizing section 20 is preferably 1.0 ⁇ 10 ⁇ 4 m 3 or more and 1.0 ⁇ 10 ⁇ 2 m 3 or less, and is preferably 2.0 ⁇ 10 ⁇ 4 m 3 or more and 1.0 ⁇ 10 m 3 or more. -3 m 3 or less is more preferable.
  • the air volume of the ventilation unit 40 is preferably 25 m 3 /h or more and 400 m 3 /h or less, and more preferably 50 m 3 /h or more and 200 m 3 /h or less.
  • the pulverizing section 20 means a region capable of pulverizing the porous polymer material, so the "volume of the pulverizing section 20" is defined as "the volume of the region capable of pulverizing the porous polymer material. ” means.
  • the static elimination effect can be improved if the above ventilation frequency is achieved in the pulverizing section 20 (i.e., the region capable of pulverizing the porous polymer body) of the pulverizing device 10, so other regions (for example, the screen 22 area outside) does not have to meet the above ventilation frequency.
  • the ventilation unit 40 may suck the polymer blocks that have passed through the sieve 16 .
  • a dust collector or the like can be used as appropriate.
  • the pulverizing device 10 includes the pulverizing unit 20 for pulverizing the porous polymer material and the irradiation unit 30 for irradiating at least part of the pulverizing unit 20 with radiation.
  • the porous polymer material being pulverized in the pulverizing section 20 is neutralized by the radiation emitted from the pulverizing section 20 . Therefore, it is possible to suppress the occurrence of scorching even in the pulverization of a porous polymer material that tends to be charged and to scorch due to its low density.
  • Micromolecule refers to a molecule of high molecular weight that has a structure made up of multiple repetitions of units derived substantially or conceptually from molecules of lower molecular weight.
  • macromolecules include polyamines, polysaccharides, polypeptides, proteins, polyamides, polyesters, polyolefins, polyethers, polynucleotides and the like.
  • a "porous body” means a solid having pores (voids) inside. A porous polymer can be obtained, for example, by removing water from a frozen polymer aqueous solution.
  • the polymeric porous body in this exemplary embodiment may contain a biocompatible substance.
  • Biocompatibility means that it does not cause significant adverse reactions such as long-term and chronic inflammatory reactions when it comes into contact with living organisms.
  • biocompatible substances include proteins and polysaccharides.
  • the polymeric porous material in this exemplary embodiment may contain protein.
  • the size of the porous polymer body before pulverization is preferably 0.1 mm or more and 50 mm or less. By setting the size of the porous polymer body before pulverization within the above range, the time required for pulverization can be shortened. In addition, it is more preferable that the size of the porous polymer body before pulverization is 0.1 mm or more and 16 mm or less.
  • the size of the porous polymer body can be defined by the square root of the projected area of the porous polymer body, that is, the length of one side of a square having the same area as the projected area.
  • the pore size of the porous polymer body is preferably 10 ⁇ m or more and 2500 ⁇ m or less, more preferably 40 ⁇ m or more and 1000 ⁇ m or less, from the viewpoint of enhancing the affinity with the living body.
  • the pore diameter of the porous polymer material can be measured as the diameter of a circle (equivalent circle diameter) having the same area as the pore when a cut surface near the center of the porous polymer material is observed with a microscope. It can be evaluated as the median value of the equivalent circle diameters of all the holes in the
  • the density of the porous polymer material is preferably 0.01 g/cm 3 or more and 0.3 g/cm 3 or less, more preferably 0.05 g/cm 3 or more and 0.1 g/cm 3 or less. preferable.
  • the density of the porous polymer can be calculated by dividing the mass of the porous polymer by the apparent volume of the porous polymer including internal voids.
  • the porosity of the porous polymer body is preferably 80.00% or more and 99.99% or less, and preferably 92.01% or more and 99.99% or less, from the viewpoint of enhancing the affinity with the living body. is more preferable.
  • the porous polymer body in this exemplary embodiment preferably contains a biodegradable polymer from the viewpoint of being used as a regenerative medicine material.
  • biodegradable polymers include polypeptides such as naturally occurring peptides, recombinant peptides, and chemically synthesized peptides (for example, gelatin etc. described below). Also included are polylactic acid, polyglycolic acid, lactic acid/glycolic acid copolymer (PLGA), hyaluronic acid, glycosaminoglycan, proteoglycan, chondroitin, cellulose, agarose, carboxymethylcellulose, chitin and chitosan.
  • the polymeric porous material in this exemplary embodiment contains a recombinant peptide.
  • non-biodegradable polymers include polytetrafluoroethylene (PTFE), polyurethane, polypropylene, polyester, vinyl chloride, polycarbonate, acrylic, silicone and MPC (2-methacryloyloxyethylphosphorylcholine).
  • polypeptides such as recombinant peptides or chemically synthesized peptides are not particularly limited as long as they have biocompatibility and biodegradability.
  • polypeptides include gelatin, collagen, elastin, fibronectin, pronectin, laminin, tenascin, fibrin, fibroin, entactin, thrombospondin and retronectin.
  • the porous polymer material in this exemplary embodiment preferably contains gelatin, collagen or atelocollagen, more preferably natural gelatin, recombinant gelatin or chemically synthesized gelatin, and further preferably contains recombinant gelatin. preferable.
  • naturally gelatin as used herein means gelatin made from naturally derived collagen.
  • Examples of natural gelatin and its recombinant gelatin include those derived from animals such as fish and mammals, but those derived from mammals are preferred. Examples of mammalian animals include humans, horses, pigs, mice, rats, and the like. When the porous polymer material according to this exemplary embodiment contains recombinant gelatin, the recombinant gelatin is more preferably derived from humans.
  • amino acid sequences constituting polypeptides are represented by one-letter code (e.g., "G” for glycine residue) or three-letter code (e.g., "Gly” for glycine residue) known in the art. express. Also, “%” for the amino acid sequence of a polypeptide is based on the number of amino acid (or imino acid) residues unless otherwise specified.
  • recombinant gelatin Recombinant gelatin having biocompatibility, biodegradability, and ability to regenerate tissues such as bones, which may be included in the porous polymer material in this exemplary embodiment, will be described below.
  • recombinant gelatin means a polypeptide or protein-like substance having an amino acid sequence similar to gelatin produced by genetic recombination technology.
  • the following recombinant gelatin can be used as a tissue repair material that contributes to the formation of tissue at the site when implanted in vivo.
  • tissue repair material is not limited to materials that contribute to the formation of normal tissue that normally exists at the implantation site, but also includes materials that promote the formation of abnormal tissue including scar tissue. .
  • the "tissue” that can be repaired by the tissue repair material may be hard tissue such as teeth and bones, and the following recombinant gelatin is particularly suitable as a base material for bone regeneration.
  • the recombinant gelatin preferably has repeats of the Gly-XY sequence characteristic of collagen.
  • the plurality of Gly-XY may be the same or different.
  • Gly-XY Gly represents a glycine residue
  • X and Y represent any amino acid residue other than glycine residues.
  • X and Y preferably contain many imino acid residues, ie, proline residues or oxyproline residues.
  • the content of such imino acid residues preferably accounts for 10% to 45% of the total gelatin.
  • the content of Gly-XY in gelatin is preferably 80% or more, more preferably 95% or more, and even more preferably 99% or more.
  • Examples of recombinant gelatin include EP1014176A2, US6992172, WO2004/85473, WO2008/103041, JP2010-519293, JP2010-519252, JP2010-518833, JP2010-519251, WO20710/128672 etc. can be used, but the present invention is not limited to these.
  • the recombinant gelatin preferably has a molecular weight of 2 kDa or more and 100 kDa or less, more preferably 5 kDa or more and 90 kDa or less, and even more preferably 10 kDa or more and 90 kDa or less.
  • the recombinant gelatin preferably further contains a cell adhesion signal, and more preferably has two or more cell adhesion signals per molecule.
  • cell adhesion signals include the RGD sequence, YIGSR sequence, PDSGR sequence, LGTIPG sequence, IKVAV sequence and HAV sequence.
  • the RGD sequence is preferred, and among the RGD sequences, the ERGD sequence is even more preferred.
  • the recombinant gelatin sequence preferably satisfies at least one of the following aspects (1-1) to (1-3).
  • the recombinant gelatin may have the following aspects (1-1) to (1-3) alone, or may have two or more aspects in combination.
  • (1-1) does not contain serine and threonine residues;
  • (1-2) does not contain serine, threonine, asparagine, tyrosine and cysteine residues;
  • (1-3) Does not contain the amino acid sequence represented by Asp-Arg-Gly-Asp.
  • the recombinant gelatin preferably has a repeating structure of A-[(Gly-XY) n ] m -B.
  • m represents 2 to 10, preferably 3 to 5.
  • a and B represent any amino acid or amino acid sequence.
  • n represents 3 to 100, preferably 15 to 70, more preferably 50 to 65.
  • the recombinant gelatin has the formula: Gly-Ala-Pro-[(Gly-XY) 63 ] 3 -Gly.
  • each of the 63 Xs independently represents any amino acid residue
  • each of the 63 Ys independently represents any of the amino acid residues.
  • the three [(Gly-XY) 63 ] may be the same or different.
  • the recombinant gelatin preferably satisfies at least one of the following aspects (2-1) to (2-4).
  • the recombinant gelatin may have the following aspects (2-1) to (2-4) alone, or may have two or more aspects in combination.
  • (2-1) the carbamoyl group is not hydrolyzed;
  • (2-2) Does not have procollagen. (2-3) does not have a telopeptide;
  • (2-4) A substantially pure collagen-like material prepared with a nucleic acid encoding native collagen.
  • Recombinant gelatin is preferably any one of the following (A) to (C) because of its high tissue repair ability.
  • a peptide consisting of the amino acid sequence of SEQ ID NO: 1 below. GAP(GAPGLQGAPGLQGMPGERGAAGLPGPKGERGDAGPKGADGAPGAPGLQGMPGERGAAGLPGPKGERGDAGPKGADGAPGKDGVRGLAGPIGPPGERGAAGLPGPKGERGDAGPKGADGAPGKDGVRGLAGPIGPPGPAGAPGAPGLQGMPGERGAAGLPGPKGERGDAGPKGADGAPGKDGVRGLAGPP) 3 G (SEQ ID NO: 1)
  • B A peptide having biocompatibility, consisting of an amino acid sequence in which one or several amino acid residues are modified (eg, deleted, substituted or added) in the amino acid sequence of SEQ ID NO:1.
  • (C) consists of an amino acid sequence having a partial sequence having 80% or more sequence identity with the partial amino acid sequence consisting of the 4th to 192nd amino acid residues in the amino acid sequence of SEQ ID NO: 1, and biocompatibility Peptides with properties.
  • the number of amino acid residues to be modified varies depending on the total number of amino acid residues of the recombinant gelatin, but is, for example, 2 to 15. is preferred, and 2 to 5 is more preferred.
  • sequence identity with respect to the amino acid sequences of the two peptides to be compared (that is, the peptide (A) and the peptide (C)) is a value calculated by the following formula. Point.
  • the comparison (alignment) of a plurality of peptides shall be performed according to a conventional method so as to maximize the number of identical amino acid residues.
  • Sequence identity (%) [(number of identical amino acid residues) / (alignment length)] x 100
  • the "partial amino acid sequence consisting of the 4th to 192nd amino acid residues” corresponds to the repeating unit in the amino acid sequence shown in SEQ ID NO:1.
  • a “partial sequence” corresponds to a repeating unit in the sequence (C) above.
  • the peptide of (C) above may contain at least one repeating unit (partial sequence) having a sequence identity of 80% or more with the repeating unit in the amino acid sequence shown in SEQ ID NO: 1, and may contain two or more. is preferred.
  • the peptide of (C) above contains a plurality of different repeating units, some of the plurality of repeating units have less than 80% sequence identity with the repeating unit in the amino acid sequence shown in SEQ ID NO: 1. may be However, in the peptide of (C) above, the total number of amino acid residues of repeating units (partial sequences) having 80% or more sequence identity with the repeating units in the amino acid sequence shown in SEQ ID NO: 1 is equal to the total number of amino acid residues 80% or more of the cardinal number is preferred.
  • sequence identity between the partial sequence in the peptide (C) and the partial amino acid sequence consisting of the 4th to 192nd amino acid residues in the amino acid sequence of SEQ ID NO: 1 is from the viewpoint of tissue repair ability. Therefore, it is preferably 90% or more, more preferably 95% or more.
  • the length of the peptide defined in (C) above can be 151 to 2260 amino acid residues, and is preferably 193 or more from the viewpoint of degradability after cross-linking. From the point of view, the number of amino acid residues is preferably 944 or less. More preferably, the number of amino acid residues is 380-756.
  • the above recombinant gelatin can be produced by genetic recombination techniques known to those skilled in the art, and may be produced, for example, according to the methods described in EP1014176A2, US6992172, WO2004/85473 and WO2008/103041. Specifically, a gene encoding an amino acid sequence of a given recombinant gelatin is obtained, incorporated into an expression vector to prepare a recombinant expression vector, and introduced into an appropriate host to prepare a transformant. . Recombinant gelatin is produced by culturing the resulting transformant in an appropriate medium, and the recombinant gelatin used in the present disclosure can be prepared by recovering the recombinant gelatin produced from the culture.
  • tissue repair can be achieved. It can be used as a material, a cell scaffold, a member for transplantation, and the like.
  • the polymeric porous body in this exemplary embodiment is not limited to being made of one material such as recombinant gelatin.
  • it may contain components that promote biological reactions such as growth factors and drugs, and other components that can contribute to tissue repair or regeneration, such as cells.
  • examples of such components include components related to bone regeneration or osteogenesis, such as osteoinductive agents.
  • osteoinductive drugs include BMP (bone morphogenetic factor) and bFGF (basic fibroblast growth factor), but are not particularly limited.
  • the polymer porous body may be applied by being mixed with an inorganic material such as hydroxyapatite or by preparing a composite.
  • the size of the polymer block (that is, the size of the porous polymer body after pulverization) is preferably 0.01 mm or more and 10 mm or less. By setting the polymer block within the above range, a size suitable for the tissue repair material can be obtained. Moreover, it is more preferably 0.1 mm or more and 5 mm or less, and still more preferably 0.3 mm or more and 1.4 mm or less.
  • the size of the polymer block means the size of each polymer block, and means a representative value (for example, average value, median value, etc.) of the sizes of a plurality of polymer blocks. not a thing
  • the size of the polymer block can be defined by the opening of the sieve when the polymer block is sieved. For example, when the polymer blocks passed through a 1.4 mm sieve are passed through a 0.3 mm sieve, the polymer blocks remaining on the sieve are treated as polymer blocks having a size of 0.3 mm or more and 1.4 mm or less. can be
  • the shape of the polymer block is not particularly limited.
  • it may be particulate (granule), amorphous, spherical, powdery, porous, fibrous, spindle-shaped, flattened and sheet-shaped. Particles (granules), amorphous, spherical, powdery and porous are preferred.
  • amorphous means that the surface shape is not uniform, and examples thereof include rock-like irregularities.
  • Example 1 (recombinant gelatin)
  • the recombinant peptide CBE3 was used as the recombinant gelatin contained in the porous polymer material.
  • CBE3 the one described below was used (described in WO2008/103041A1).
  • Molecular weight 51.6 kD Structure: GAP[(GXY) 63 ] 3G Number of amino acids: 571 RGD sequence: 12
  • CBE3 has an ERGD sequence.
  • the amino acid sequence of CBE3 does not contain serine, threonine, asparagine, tyrosine and cysteine residues.
  • Isoelectric point 9.34 Hydrophilic repeating unit ratio in polymer: 26.1% Amino acid sequence (SEQ ID NO: 1) 3G
  • a cylindrical cup-shaped container made of an aluminum alloy (JIS A5056 alloy) was prepared.
  • the container has a closed side surface and a bottom surface and an open top surface when the curved surface is used as a side surface.
  • the bottom surface has a thickness of 5 mm, and the inner circumference of the bottom surface is chamfered with R2 mm.
  • the inside of the side and bottom surfaces is coated with FEP (tetrafluoroethylene-hexafluoropropylene copolymer), resulting in an internal diameter of 104 mm.
  • This container is hereinafter referred to as a "cylindrical container”.
  • a frozen gelatin body was obtained by standing still.
  • the freeze-dried gelatin was freeze-dried using a freeze dryer (DFR-5N-B manufactured by ULVAC, Inc.) to remove moisture, thereby preparing a freeze-dried product.
  • This freeze-dried material is an example of the porous polymer material of the present disclosure.
  • the freeze-dried product had a porosity of 93.9%, a pore size of 50 ⁇ m, and a density of 0.0748 g/cm 3 .
  • the porous polymer material was manually broken into pieces of 20 mm square or less and equilibrated in an environment of 23°C and 50% RH.
  • the porous polymer material after being broken by hand is arranged on a graph paper with a gray ruled line on a black background (both the black background and the gray ruled line are lower in brightness than the porous polymer material), and photographed from the direction perpendicular to the paper surface. did.
  • the photographed image is binarized by image processing so that the porous polymer body region and the rest can be separated, the region corresponding to the porous polymer body (that is, the projected area of the porous polymer body) is specified, and the projected area is determined.
  • ImageJ manufactured by National Institutes of Health
  • a screen 22 with a hole diameter of 0.079 inch was used.
  • the ventilation unit 40 was operated so that the air velocity in the holes of the screen 22 was 8.5 ⁇ 0.1 m/s, and the rotary impeller 24 was rotated at a rotation speed of 1100 ⁇ 10 rpm to implement pulverization.
  • the wind speed was measured using a digital anemometer (CW-60 manufactured by Custom Co., Ltd.).
  • a rake type (7A1611) rotary impeller was used.
  • the amount of air sucked by the ventilation unit 40 was 106 m 3 /h, and the ventilation frequency was 2.6 ⁇ 10 5 times/h.
  • the volume of the trapezoidal rotating body area inside the screen 22 was 4.1 ⁇ 10 ⁇ 4 m 3 .
  • a screen 22 with a hole diameter of 0.040 inch was used.
  • the ventilation part 40 was operated so that the air velocity in the holes of the screen 22 was 10.0 ⁇ 0.1 m/s, and the rotating impeller 24 was rotated at a rotation speed of 2200 ⁇ 10 rpm to implement pulverization.
  • the wind speed was measured using a digital anemometer (CW-60 manufactured by Custom Co., Ltd.).
  • a rake type (7A1611) rotary impeller was used.
  • the amount of air sucked by the ventilation unit 40 was 107 m 3 /h, and the ventilation frequency was 2.6 ⁇ 10 5 times/h.
  • the volume of the trapezoidal rotating body area inside the screen 22 was 4.1 ⁇ 10 ⁇ 4 m 3 .
  • the fraction of the polymer block under the sieve with a mesh size of 1.4 mm and above the sieve with a mesh size of 0.3 mm was recovered and placed in a 10 mL glass vial (barrel diameter: 24.3 mm). 5 ⁇ 3.0 mg was filled.
  • the irradiation part 30 irradiated the crushing part 20 with X-rays (soft X-rays) with a tube voltage of 15 kV.
  • X-rays soft X-rays
  • FIG. 1 two irradiation units 30A and 30B are provided at an angle of 60 degrees to the left and right with respect to the vertical direction (one-dot chain line) of the crushing device 10, and the discharge unit 14 side Soft X-rays were applied to the pulverization unit 20 by a total of three irradiation units including the provided irradiation unit 30C.
  • film (hereinafter referred to as "film") was placed and irradiated with soft X-rays.
  • a dedicated kit Panher System manufactured by Hanshin Technical Research Institute Co., Ltd.
  • the film was developed, fixed, and hardened, etc., and a transmission densitometer (310 manufactured by X-Rite) was used to test the film. Transmission density was measured.
  • irradiation with soft X-rays and measurement of transmission density were repeated while changing the irradiation time.
  • Fig. 2 shows a characteristic curve (dashed line) obtained from the measurement results of transmission density at a distance where the irradiation dose is known.
  • the characteristic curve is represented by plotting the relative irradiation dose (logarithm of the relative X-ray dose) on the horizontal axis and the transmission density on the vertical axis. By using this characteristic curve, the relative irradiation dose can be derived from the transmission density.
  • FIG. 2 also shows an approximation straight line (solid line) obtained by linearly approximating the linear region of the characteristic curve.
  • a film similar to the above was placed inside the screen 22 in the pulverizing section 20, and was irradiated with soft X-rays for 6400 seconds by the three irradiation sections 30A to 30C. Further, the film was developed, fixed, hardened, etc. in the same manner as above, and the transmission density of the film was measured. Based on the approximate straight line of the characteristic curve in FIG. was derived. Table 1 shows the result of derivation of the irradiation dose (70 ⁇ m dose equivalent rate) inside the screen 22 . Since the irradiation dose derived in this manner indicates the irradiation dose inside the screen 22 , it can be regarded as the irradiation dose applied to the porous polymer material being pulverized in the pulverizing section 20 .
  • FIG. 3 shows the configuration of the pulverizing device 10 according to the second embodiment.
  • the irradiation angle of the irradiation section 30B with respect to the crushing section 20 is different from that in the first embodiment (see FIG. 1).
  • two irradiation units 30A and 30B provided at angles of 60 degrees and 5 degrees on one side with respect to the vertical direction (one-dot chain line) of the crushing device 10, and a discharge unit
  • the pulverization unit 20 was irradiated with soft X-rays by a total of three irradiation units including the irradiation unit 30C provided on the unit 14 side.
  • Example 1 In order to verify the effects of the irradiation unit 30 in removing static electricity and suppressing scorching, the porous polymer material was pulverized in Example 1 without irradiation with soft X-rays by the irradiation unit 30 .
  • the other apparatus configuration, recombinant gelatin, method for producing porous polymer, method for pulverizing porous polymer, and method for evaluating scorching of polymer blocks are the same as in Example 1 above, so description thereof is omitted. do.
  • Table 1 shows the proportion of glass vials in which scorching was observed in Comparative Example 1.
  • Example 1 As shown in Table 1, the percentage of burning in Example 1 is 60%, the percentage of burning in Example 2 is 33%, and the percentage of burning in Comparative Example 1 is 90%. and 2, it was found that scorching was suppressed. From this, it can be seen that according to the method and pulverization apparatus 10 of the present disclosure, charring that occurs when pulverizing a porous polymer body can be suppressed by irradiating soft X-rays.
  • Burning may be detected, for example, by detecting a colored portion of the polymer block by image inspection. Alternatively, for example, it may be performed by detecting a change in the chemical structure of the polymer block by a spectroscopic technique such as infrared spectroscopy.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)
  • Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)
PCT/JP2022/013691 2021-03-31 2022-03-23 粉砕方法、高分子ブロック製造方法及び粉砕装置 Ceased WO2022210204A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2023511086A JPWO2022210204A1 (https=) 2021-03-31 2022-03-23
EP22780418.4A EP4299182A4 (en) 2021-03-31 2022-03-23 CRUSHING PROCESS, POLYMER BLOCK PRODUCTION PROCESS AND CRUSHING DEVICE
CN202280023461.3A CN117042884A (zh) 2021-03-31 2022-03-23 粉碎方法、高分子嵌段物制造方法及粉碎装置
US18/477,630 US20240017267A1 (en) 2021-03-31 2023-09-29 Pulverizing method, method for producing polymer block, and pulverization device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2021-060910 2021-03-31
JP2021060910 2021-03-31

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US18/477,630 Continuation US20240017267A1 (en) 2021-03-31 2023-09-29 Pulverizing method, method for producing polymer block, and pulverization device

Publications (1)

Publication Number Publication Date
WO2022210204A1 true WO2022210204A1 (ja) 2022-10-06

Family

ID=83456804

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2022/013691 Ceased WO2022210204A1 (ja) 2021-03-31 2022-03-23 粉砕方法、高分子ブロック製造方法及び粉砕装置

Country Status (5)

Country Link
US (1) US20240017267A1 (https=)
EP (1) EP4299182A4 (https=)
JP (1) JPWO2022210204A1 (https=)
CN (1) CN117042884A (https=)
WO (1) WO2022210204A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117282507A (zh) * 2023-11-24 2023-12-26 昆明理工大学 一种通过识别球磨机内惰性区面积选择最优的磨矿介质配比方法
JP2025054196A (ja) * 2023-09-25 2025-04-07 エコプロ ビーエム カンパニー リミテッド 静電気除去が可能なジェットミル及びこれを用いた硫化物系固体電解質の粉砕方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3160613A1 (fr) * 2024-03-29 2025-10-03 Faurecia Sièges d'Automobile Procédé de densification de matériaux souples

Citations (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4895647A (https=) * 1972-03-21 1973-12-07
JPS5592667A (en) 1978-11-17 1980-07-14 Iwatani & Co Preparation of konjak powder
JPS5730556A (en) * 1980-07-30 1982-02-18 Showa Tansan Kk Method of crushing flammable substance
JPH02170827A (ja) * 1988-12-23 1990-07-02 Ain Kk ゼラチンの粉砕方法、塗料、合成樹脂溶液、皮膜並びにフィルム及び加工布
JPH052078A (ja) * 1991-02-15 1993-01-08 Toshiba Glass Co Ltd 蛍光ガラス線量計
JPH09176A (ja) 1995-06-22 1997-01-07 Nakano Vinegar Co Ltd こんにゃく粉の微粉砕方法及び微粉末を用いたこんにゃく又はこんにゃく含有食品の製造方法
JPH0961400A (ja) * 1995-08-24 1997-03-07 Mita Ind Co Ltd 粉体の接触電位差の測定方法
JPH11320558A (ja) * 1998-03-18 1999-11-24 Idemitsu Petrochem Co Ltd 熱硬化性樹脂の粉砕方法
EP1014176A2 (en) 1998-12-23 2000-06-28 Fuji Photo Film B.V. Silver halide emulsions containing recombinant gelatin-like proteins
JP2001257096A (ja) * 2000-03-10 2001-09-21 Techno Ryowa Ltd 静電気対策用吹出口
WO2004085473A2 (en) 2003-03-28 2004-10-07 Fuji Photo Film B.V. Rgd-enriched gelatine-like proteins with enhanced cell binding
US6992172B1 (en) 1999-11-12 2006-01-31 Fibrogen, Inc. Recombinant gelatins
WO2006068165A1 (ja) * 2004-12-21 2006-06-29 Eisai R & D Management Co., Ltd. 流動層装置
JP2007194371A (ja) * 2006-01-18 2007-08-02 Fujifilm Corp 基板ユニットの除電方法並びに固体撮像装置の製造方法及び製造装置
WO2008103041A1 (en) 2007-02-21 2008-08-28 Fujifilm Manufacturing Europe B.V. Recombinant gelatins
WO2010128672A1 (ja) 2009-05-07 2010-11-11 富士フイルム株式会社 遺伝子組み換えゼラチンを含む血管新生誘導剤
WO2010147109A1 (ja) 2009-06-15 2010-12-23 富士フイルム株式会社 遺伝子組み換えゼラチン及び塩基性線維芽細胞増殖因子を含む血管新生誘導剤
WO2014133081A1 (ja) 2013-02-27 2014-09-04 富士フイルム株式会社 細胞移植用細胞構造体、生体親和性高分子ブロック及びそれらの製造方法
WO2017213170A1 (ja) * 2016-06-08 2017-12-14 富士フイルム株式会社 ゼラチン成形体の製造方法及びゼラチン成形体
JP2021060910A (ja) 2019-10-09 2021-04-15 Kddi株式会社 端末装置、システム、通過数測定方法、及びコンピュータプログラム
JP2022013691A (ja) 2020-06-30 2022-01-18 ケンテック株式会社 鉄筋付きデッキプレート

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3562213B2 (ja) * 1997-05-27 2004-09-08 宇部興産株式会社 竪型粉砕機
JP2004249173A (ja) * 2003-02-18 2004-09-09 Seiko Epson Corp 粉体処理装置、粉体処理方法、トナーの製造方法およびトナー
JP5070933B2 (ja) * 2006-05-18 2012-11-14 三菱化学株式会社 下引き層形成用塗布液、下引き層形成用塗布液の製造方法、電子写真感光体、画像形成装置及び電子写真カートリッジ
JP4283861B2 (ja) * 2006-11-09 2009-06-24 シャープ株式会社 樹脂粒子の製造方法
JP5578826B2 (ja) * 2009-10-02 2014-08-27 協和産業株式会社 廃プラスチックの選別分離方法および選別分離設備
JP5666378B2 (ja) * 2010-05-24 2015-02-12 信越化学工業株式会社 非水電解質二次電池用負極活物質の製造方法及び非水電解質二次電池用負極活物質並びに非水電解質二次電池用負極材、非水電解質二次電池用負極、非水電解質二次電池
JP5934974B1 (ja) * 2015-11-18 2016-06-15 大村塗料株式会社 バイオナノファイバーの製造方法

Patent Citations (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4895647A (https=) * 1972-03-21 1973-12-07
JPS5592667A (en) 1978-11-17 1980-07-14 Iwatani & Co Preparation of konjak powder
JPS5730556A (en) * 1980-07-30 1982-02-18 Showa Tansan Kk Method of crushing flammable substance
JPH02170827A (ja) * 1988-12-23 1990-07-02 Ain Kk ゼラチンの粉砕方法、塗料、合成樹脂溶液、皮膜並びにフィルム及び加工布
JPH052078A (ja) * 1991-02-15 1993-01-08 Toshiba Glass Co Ltd 蛍光ガラス線量計
JPH09176A (ja) 1995-06-22 1997-01-07 Nakano Vinegar Co Ltd こんにゃく粉の微粉砕方法及び微粉末を用いたこんにゃく又はこんにゃく含有食品の製造方法
JPH0961400A (ja) * 1995-08-24 1997-03-07 Mita Ind Co Ltd 粉体の接触電位差の測定方法
JPH11320558A (ja) * 1998-03-18 1999-11-24 Idemitsu Petrochem Co Ltd 熱硬化性樹脂の粉砕方法
EP1014176A2 (en) 1998-12-23 2000-06-28 Fuji Photo Film B.V. Silver halide emulsions containing recombinant gelatin-like proteins
US6992172B1 (en) 1999-11-12 2006-01-31 Fibrogen, Inc. Recombinant gelatins
JP2001257096A (ja) * 2000-03-10 2001-09-21 Techno Ryowa Ltd 静電気対策用吹出口
WO2004085473A2 (en) 2003-03-28 2004-10-07 Fuji Photo Film B.V. Rgd-enriched gelatine-like proteins with enhanced cell binding
WO2006068165A1 (ja) * 2004-12-21 2006-06-29 Eisai R & D Management Co., Ltd. 流動層装置
JP2007194371A (ja) * 2006-01-18 2007-08-02 Fujifilm Corp 基板ユニットの除電方法並びに固体撮像装置の製造方法及び製造装置
WO2008103041A1 (en) 2007-02-21 2008-08-28 Fujifilm Manufacturing Europe B.V. Recombinant gelatins
JP2010519293A (ja) 2007-02-21 2010-06-03 フジフィルム・マニュファクチュアリング・ヨーロッパ・ベスローテン・フエンノートシャップ 機能性が増大した非天然の組換えゼラチン
JP2010519251A (ja) 2007-02-21 2010-06-03 フジフィルム・マニュファクチュアリング・ヨーロッパ・ベスローテン・フエンノートシャップ 組換えゼラチン
JP2010519252A (ja) 2007-02-21 2010-06-03 フジフィルム・マニュファクチュアリング・ヨーロッパ・ベスローテン・フエンノートシャップ Ｒｇｄを含有する組換えゼラチン
JP2010518833A (ja) 2007-02-21 2010-06-03 フジフィルム・マニュファクチュアリング・ヨーロッパ・ベスローテン・フエンノートシャップ 高い安定性を有する組換えｘｒｇｄ富化ゼラチン
WO2010128672A1 (ja) 2009-05-07 2010-11-11 富士フイルム株式会社 遺伝子組み換えゼラチンを含む血管新生誘導剤
WO2010147109A1 (ja) 2009-06-15 2010-12-23 富士フイルム株式会社 遺伝子組み換えゼラチン及び塩基性線維芽細胞増殖因子を含む血管新生誘導剤
WO2014133081A1 (ja) 2013-02-27 2014-09-04 富士フイルム株式会社 細胞移植用細胞構造体、生体親和性高分子ブロック及びそれらの製造方法
WO2017213170A1 (ja) * 2016-06-08 2017-12-14 富士フイルム株式会社 ゼラチン成形体の製造方法及びゼラチン成形体
JP2021060910A (ja) 2019-10-09 2021-04-15 Kddi株式会社 端末装置、システム、通過数測定方法、及びコンピュータプログラム
JP2022013691A (ja) 2020-06-30 2022-01-18 ケンテック株式会社 鉄筋付きデッキプレート

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP4299182A4

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2025054196A (ja) * 2023-09-25 2025-04-07 エコプロ ビーエム カンパニー リミテッド 静電気除去が可能なジェットミル及びこれを用いた硫化物系固体電解質の粉砕方法
CN117282507A (zh) * 2023-11-24 2023-12-26 昆明理工大学 一种通过识别球磨机内惰性区面积选择最优的磨矿介质配比方法
CN117282507B (zh) * 2023-11-24 2024-02-13 昆明理工大学 一种通过识别球磨机内惰性区面积选择最优的磨矿介质配比方法

Also Published As

Publication number Publication date
EP4299182A1 (en) 2024-01-03
EP4299182A4 (en) 2024-08-28
CN117042884A (zh) 2023-11-10
US20240017267A1 (en) 2024-01-18
JPWO2022210204A1 (https=) 2022-10-06

Similar Documents

Publication Publication Date Title
US20240017267A1 (en) Pulverizing method, method for producing polymer block, and pulverization device
EP2543397B1 (en) Cell structure comprising cells and macromolecular blocks having biocompatibility
EP2517738B1 (en) A collagen/hydroxyapatite composite scaffold
Hosseinkhani et al. Bone regeneration on a collagen sponge self-assembled peptide-amphiphile nanofiber hybrid scaffold
ES2691749T3 (es) Tejido no tejido que contiene material protésico óseo
La et al. Systemically replicated organic and inorganic bony microenvironment for new bone formation generated by a 3D printing technology
JPH09505305A (ja) 骨形成促進生成物および骨形成方法
CA3011306A1 (en) Cellular blend for the regeneration of chondrocytes or cartilage type cells
Pan et al. Bone induction by biomimetic PLGA copolymer loaded with a novel synthetic RADA16-P24 peptide in vivo
CN105025941B (zh) 组织修复材料
KR101638834B1 (ko) 조직 수복재의 제조 방법
Tamaddon et al. The need for hierarchical scaffolds in bone tissue engineering
Riazi Moghadam et al. A biomimetic three-layered fibrin gel/PLLA nanofibers composite as a potential scaffold for articular cartilage tissue engineering application
US20240050966A1 (en) Powder supply device
Sindhusha et al. The role of chitosan and gelatin-based scaffolds in bone regeneration: a systematic review
EP3272370B1 (en) Cartilage-regenerating material
CN111050815B (zh) 血管新生剂及其制造方法
JP2020083796A (ja) コラーゲン様ペプチド及びリン酸カルシウムの複合体並びにこれを含む生体組織修復材
ES3012401T3 (en) Porous complex and bone regeneration material
EP3320925B1 (en) Cell structure and method for producing cell structure
EP4480509A1 (en) Tissue restoration material and tissue restoration material production method
JP7036839B2 (ja) フィブリン組成物、再生医療用基材、フィブリン組成物の製造方法およびキット
AU2020410540A1 (en) Use of hyaluronic acid derivatives in the regeneration of bone and cartilage tissues
CN118804770A (zh) 组织修复材料及组织修复材料的制造方法
EP4393453A1 (en) Tissue restorative material kit and tissue restoration method

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 22780418

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 202280023461.3

Country of ref document: CN

WWE Wipo information: entry into national phase

Ref document number: 2023511086

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2022780418

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2022780418

Country of ref document: EP

Effective date: 20230926

NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Ref document number: 2022780418

Country of ref document: EP