WO2022143716A1 - Procédé de mesure de teneur en polysaccharide de ganoderma lucidum - Google Patents
Procédé de mesure de teneur en polysaccharide de ganoderma lucidum Download PDFInfo
- Publication number
- WO2022143716A1 WO2022143716A1 PCT/CN2021/142290 CN2021142290W WO2022143716A1 WO 2022143716 A1 WO2022143716 A1 WO 2022143716A1 CN 2021142290 W CN2021142290 W CN 2021142290W WO 2022143716 A1 WO2022143716 A1 WO 2022143716A1
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- WIPO (PCT)
- Prior art keywords
- solution
- add
- monosaccharide
- concentration
- prepare
- Prior art date
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 185
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 165
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 165
- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 145
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 145
- 238000000034 method Methods 0.000 title claims abstract description 143
- 238000004128 high performance liquid chromatography Methods 0.000 claims abstract description 105
- 239000000203 mixture Substances 0.000 claims abstract description 95
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 claims abstract description 44
- 235000000346 sugar Nutrition 0.000 claims abstract description 44
- MYKOKMFESWKQRX-UHFFFAOYSA-N 10h-anthracen-9-one;sulfuric acid Chemical compound OS(O)(=O)=O.C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 MYKOKMFESWKQRX-UHFFFAOYSA-N 0.000 claims abstract description 42
- 238000004458 analytical method Methods 0.000 claims abstract description 26
- 239000000126 substance Substances 0.000 claims abstract description 10
- 150000002772 monosaccharides Chemical class 0.000 claims description 174
- 239000000243 solution Substances 0.000 claims description 161
- 239000011550 stock solution Substances 0.000 claims description 140
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 123
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 110
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- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 claims description 85
- 238000002360 preparation method Methods 0.000 claims description 85
- 239000012088 reference solution Substances 0.000 claims description 84
- 238000001514 detection method Methods 0.000 claims description 75
- 239000013558 reference substance Substances 0.000 claims description 73
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 68
- 239000008103 glucose Substances 0.000 claims description 67
- 238000012360 testing method Methods 0.000 claims description 64
- 229930182830 galactose Natural products 0.000 claims description 62
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 claims description 61
- 229940097043 glucuronic acid Drugs 0.000 claims description 61
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 60
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 58
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 claims description 55
- 239000007864 aqueous solution Substances 0.000 claims description 52
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 50
- 238000002835 absorbance Methods 0.000 claims description 45
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 36
- 239000000047 product Substances 0.000 claims description 33
- 239000012085 test solution Substances 0.000 claims description 33
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 claims description 32
- 239000012086 standard solution Substances 0.000 claims description 32
- QELUYTUMUWHWMC-UHFFFAOYSA-N edaravone Chemical compound O=C1CC(C)=NN1C1=CC=CC=C1 QELUYTUMUWHWMC-UHFFFAOYSA-N 0.000 claims description 30
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 claims description 29
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 claims description 28
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 claims description 28
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 28
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 claims description 27
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 claims description 27
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 claims description 27
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 claims description 27
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 claims description 27
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 27
- 238000006243 chemical reaction Methods 0.000 claims description 23
- 239000012488 sample solution Substances 0.000 claims description 23
- RJGDLRCDCYRQOQ-UHFFFAOYSA-N anthrone Chemical compound C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 RJGDLRCDCYRQOQ-UHFFFAOYSA-N 0.000 claims description 21
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 20
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 17
- 238000000605 extraction Methods 0.000 claims description 17
- 239000000523 sample Substances 0.000 claims description 17
- 238000010828 elution Methods 0.000 claims description 16
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 claims description 15
- 239000004677 Nylon Substances 0.000 claims description 12
- SRBFZHDQGSBBOR-STGXQOJASA-N alpha-D-lyxopyranose Chemical compound O[C@@H]1CO[C@H](O)[C@@H](O)[C@H]1O SRBFZHDQGSBBOR-STGXQOJASA-N 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- 229920001778 nylon Polymers 0.000 claims description 12
- 239000002904 solvent Substances 0.000 claims description 12
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 11
- 230000014759 maintenance of location Effects 0.000 claims description 10
- 239000008055 phosphate buffer solution Substances 0.000 claims description 10
- 239000011259 mixed solution Substances 0.000 claims description 8
- 238000003860 storage Methods 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000005457 ice water Substances 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- YTBSYETUWUMLBZ-QWWZWVQMSA-N D-threose Chemical compound OC[C@@H](O)[C@H](O)C=O YTBSYETUWUMLBZ-QWWZWVQMSA-N 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- KCOPAESEGCGTKM-UHFFFAOYSA-N 1,3-oxazol-4-one Chemical compound O=C1COC=N1 KCOPAESEGCGTKM-UHFFFAOYSA-N 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N pyridine Substances C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims description 2
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 claims 19
- AEMOLEFTQBMNLQ-YMDCURPLSA-N D-galactopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-YMDCURPLSA-N 0.000 claims 8
- 150000008163 sugars Chemical class 0.000 abstract description 6
- 238000004451 qualitative analysis Methods 0.000 abstract description 2
- 238000004445 quantitative analysis Methods 0.000 abstract description 2
- 229960001031 glucose Drugs 0.000 description 53
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 42
- 230000007062 hydrolysis Effects 0.000 description 23
- 238000006460 hydrolysis reaction Methods 0.000 description 23
- 239000012071 phase Substances 0.000 description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 21
- IAJILQKETJEXLJ-RSJOWCBRSA-N aldehydo-D-galacturonic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-RSJOWCBRSA-N 0.000 description 15
- 238000001212 derivatisation Methods 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 238000005457 optimization Methods 0.000 description 9
- 229960003082 galactose Drugs 0.000 description 8
- 229940041290 mannose Drugs 0.000 description 8
- 239000000463 material Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 230000002378 acidificating effect Effects 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 238000004737 colorimetric analysis Methods 0.000 description 3
- 238000012869 ethanol precipitation Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- 238000005903 acid hydrolysis reaction Methods 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 238000005238 degreasing Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- -1 glucose Chemical class 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- NNZXDXMEXBYSRF-UHFFFAOYSA-N 2-methyl-4h-pyrazol-3-one Chemical compound CN1N=CCC1=O NNZXDXMEXBYSRF-UHFFFAOYSA-N 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- SRBFZHDQGSBBOR-AGQMPKSLSA-N D-lyxopyranose Chemical compound O[C@@H]1COC(O)[C@@H](O)[C@H]1O SRBFZHDQGSBBOR-AGQMPKSLSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- PNNNRSAQSRJVSB-KCDKBNATSA-N aldehydo-L-fucose Chemical compound C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-KCDKBNATSA-N 0.000 description 1
- PNNNRSAQSRJVSB-BXKVDMCESA-N aldehydo-L-rhamnose Chemical compound C[C@H](O)[C@H](O)[C@@H](O)[C@@H](O)C=O PNNNRSAQSRJVSB-BXKVDMCESA-N 0.000 description 1
- AEMOLEFTQBMNLQ-WAXACMCWSA-N alpha-D-glucuronic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-WAXACMCWSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PQMKYFCFSA-N alpha-D-mannose Chemical compound OC[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-PQMKYFCFSA-N 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- AEMOLEFTQBMNLQ-UHFFFAOYSA-N beta-D-galactopyranuronic acid Natural products OC1OC(C(O)=O)C(O)C(O)C1O AEMOLEFTQBMNLQ-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-KLVWXMOXSA-N beta-L-arabinopyranose Chemical compound O[C@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-KLVWXMOXSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
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- 238000005342 ion exchange Methods 0.000 description 1
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- 239000002398 materia medica Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Procédé de mesure de teneur en polysaccharide de Ganoderma lucidum. Le procédé consiste à déterminer la composition de sucre d'un polysaccharide de Ganoderma lucidum au moyen d'une chromatographie en phase liquide haute performance, puis à préparer une solution de substance standard en fonction des sucres de composition et de leur proportion de composition, et à mesurer la teneur en polysaccharide de Ganoderma lucidum à l'aide d'un procédé phénol-acide sulfurique et/ou d'un procédé anthrone-acide sulfurique. Le procédé peut être utilisé pour l'analyse qualitative et quantitative d'un polysaccharide de Ganoderma lucidum, et fournit une référence pour un procédé d'analyse de qualité d'un polysaccharide de Ganoderma lucidum.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011609657.X | 2020-12-30 | ||
| CN202011609657 | 2020-12-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2022143716A1 true WO2022143716A1 (fr) | 2022-07-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CN2021/142290 WO2022143716A1 (fr) | 2020-12-30 | 2021-12-29 | Procédé de mesure de teneur en polysaccharide de ganoderma lucidum |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN114689736A (fr) |
| WO (1) | WO2022143716A1 (fr) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115266991A (zh) * | 2022-08-02 | 2022-11-01 | 绍兴市食品药品检验研究院 | 一种同时检测黄酒中多种甜味剂的检测方法 |
| CN115372501A (zh) * | 2022-07-27 | 2022-11-22 | 广州科曼生物科技有限公司 | 一种浙贝母或湖北贝母对照提取物及其制备方法和应用 |
| CN116875657A (zh) * | 2023-04-14 | 2023-10-13 | 上海腾瑞制药股份有限公司 | 一种重组巴曲酶的糖蛋白的定量分析方法 |
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|---|---|---|---|---|
| EP0094161A1 (fr) * | 1982-05-07 | 1983-11-16 | Imperial Chemical Industries Plc | Méthode pour la détermination de la teneur en glucose de fluides |
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| CN102495167A (zh) * | 2011-12-09 | 2012-06-13 | 劲牌有限公司 | 枸杞多糖提取物中枸杞多糖的检测方法 |
| CN104458985A (zh) * | 2014-10-14 | 2015-03-25 | 中国药科大学 | 枸杞多糖多元指纹图谱的构建方法及其标准指纹图谱 |
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| CN107602719A (zh) * | 2017-10-20 | 2018-01-19 | 广东粤微食用菌技术有限公司 | 一种具有显著辅助抗肿瘤活性的灵芝子实体精制多糖及其制备方法和应用 |
| CN107632096A (zh) * | 2017-10-19 | 2018-01-26 | 佛山科学技术学院 | 一种测定防风多糖的单糖组成的方法 |
| US20200158696A1 (en) * | 2017-05-29 | 2020-05-21 | The Affiliated Hospital Of Qingdao University | Detection of free mannose and glucose in serum using high performance liquid chromatography |
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2021
- 2021-12-29 CN CN202111636098.6A patent/CN114689736A/zh active Pending
- 2021-12-29 WO PCT/CN2021/142290 patent/WO2022143716A1/fr active Application Filing
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0094161A1 (fr) * | 1982-05-07 | 1983-11-16 | Imperial Chemical Industries Plc | Méthode pour la détermination de la teneur en glucose de fluides |
| CN102008515A (zh) * | 2010-11-26 | 2011-04-13 | 江南大学 | 一种灵芝孢子粉多糖指纹图谱的构建方法及其标准指纹图谱 |
| CN102495167A (zh) * | 2011-12-09 | 2012-06-13 | 劲牌有限公司 | 枸杞多糖提取物中枸杞多糖的检测方法 |
| CN104458985A (zh) * | 2014-10-14 | 2015-03-25 | 中国药科大学 | 枸杞多糖多元指纹图谱的构建方法及其标准指纹图谱 |
| CN104730009A (zh) * | 2015-02-27 | 2015-06-24 | 浙江经贸职业技术学院 | 一种茶树花中多糖含量的检测方法 |
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