WO2022110574A1

WO2022110574A1 - Liquid injection and suction device for measurement chamber

Info

Publication number: WO2022110574A1
Application number: PCT/CN2021/081003
Authority: WO
Inventors: 胡统号; 苗新利; 吴冬; 吕瑞
Original assignee: 苏州长光华医生物医学工程有限公司
Priority date: 2020-11-30
Filing date: 2021-03-16
Publication date: 2022-06-02
Also published as: CN112378855A

Abstract

A liquid injection and suction device for a measurement chamber, comprising: a support (1) fixed on a measurement chamber cover plate (8), wherein a rotating disc (7) is provided in the measurement chamber, a plurality of reaction cup (6) accommodating positions arranged in the circumferential direction of the rotating disc (7) are formed in the rotating disc (7), and a driving member drives the rotating disc (7) to rotate, so that reaction cups (6) can pass through a liquid injection position and a liquid suction position in sequence; a needle assembly (3), comprising a liquid suction needle (32), a liquid injection needle (33), and a needle fixing plate (31), wherein the liquid suction needle (32) and the liquid injection needle (33) respectively pass through the measurement chamber cover plate (8) and correspond to reaction cups (6) on the liquid suction position and the liquid injection position; and a driving assembly (2), comprising a lead screw motor (21), a transmission lead screw (28), a lead screw nut (29) fixedly connected to the needle fixing plate (31), a driving seat (23) fixed on the needle fixing plate (31), a linear guide rail provided on the support (1), and a sliding block (26) fixed on the driving seat (23), wherein one end of the transmission lead screw (28) is connected to the output shaft of the lead screw motor (21), and the other end is connected to the measurement chamber cover plate (8) by means of a bearing (22). The liquid injection and suction device for the measurement chamber saves the test time, and realizes the dry-wet separation treatment of a waste liquid and the used reaction cup (6).

Description

A liquid injection and suction device in a measuring chamber

technical field

The application belongs to the technical field of medical devices, and in particular relates to a liquid injection and liquid suction device in a measuring chamber.

Background technique

At present, the most commonly used diagnostic method in the medical field - in vitro diagnosis, refers to the collection of human body fluids, excrement, secretions for chemical composition or chemical reaction analysis, so as to determine human diseases, such as chemiluminescence immunoassay, molecular diagnosis, etc. , These diagnostic methods all use automatic or semi-automatic instruments to add samples, analyze, and give a diagnosis report. Such instruments have a functional unit—a detection unit (ie, a measurement room).

On a fully automatic chemiluminescence instrument, the measurement unit (ie, the measurement chamber) is a closed dark container structure. The luminescence value of the sample can be measured by a precision photon counter to calculate the concentration of the detected substance. Before testing the sample to be tested, it is necessary to Inject some buffer solution (exciter) to stimulate the analyte to emit light. These buffer solutions are acidic and basic and need to be injected into the reaction cup accurately. During high-speed injection, splashing cannot be avoided, so as not to corrode the structure of the closed chamber of the measurement chamber. cause instrument failure. In addition, there are acid-base solutions and samples in the tested samples, which are biological safety hazards. Therefore, the tested mixed waste liquid needs to be recycled and centralized, so that the waste liquid and the used reaction cup can be separated from wet and dry.

The measuring device given in the patent (document number CN107167427A) "a single reaction cup luminescence measuring device" applied by our company can only measure a single reaction cup. During the measurement, the starting agent and the activating agent are added to the reaction cup through the reagent adding tube. , after the measurement, open the sealed chamber door to take out the reaction cup with waste liquid, and then perform the next test, so that when one reaction cup in the measurement room is measured, the other reaction cup stations are in a waiting state, which greatly reduces the instrument's Test speed; at the same time, after the luminescence measurement is completed, the waste liquid and the used cuvette are not treated separately, which greatly increases the cost and time of post-processing.

Utility model content

The technical problem to be solved by the utility model is: in order to solve the problems of low testing speed of the measuring chamber and high post-processing cost of the used reaction cup in the prior art, a liquid injection and liquid suction device for the measuring chamber is provided.

The technical scheme adopted by the utility model to solve its technical problems is:

A liquid injection and suction device for a measuring chamber, characterized in that it includes:

The bracket is fixed on the cover plate of the measuring chamber at the top of the measuring chamber. The measuring chamber is provided with a turntable. The turntable is provided with a plurality of reaction cup accommodating positions arranged along the circumference of the turntable for accommodating the reaction cups, and the turntable is driven to rotate by the driving member The cuvette in the cuvette storage position can pass through the liquid injection level and the liquid suction level successively;

Needle assembly, including suction needle, liquid injection needle and a needle fixing plate on the upper part of the cover plate of the measuring chamber fixed with the suction needle and the liquid injection needle, the suction needle and the liquid injection needle respectively pass through the cover plate of the measuring chamber And corresponding to the reaction cup on the suction level and injection level;

The drive assembly includes a screw motor, a transmission screw, a screw nut that is screwed on the transmission screw and is fastened to the needle fixing plate, a drive seat that is fixed to the needle fixing plate, and is arranged on the bracket and the transmission The linear guide rail parallel to the screw rod and the slider fixed on the drive seat and matched with the linear guide rail, one end of the drive screw rod is connected to the output shaft of the screw motor, and the other end is connected with the cover plate of the measuring chamber through the bearing, and the screw motor, The drive screw can move the suction needle and the liquid injection needle fixed on the needle fixing plate up and down, and drive the slider to move up and down along the linear guide through the needle fixing plate and the driving seat.

Preferably, the tip of the liquid aspiration needle and the tip of the liquid injection needle have a certain height difference; preferably, the liquid injection and liquid aspiration device in the measurement chamber further comprises a liquid injection needle sealing assembly and a liquid aspiration needle sealing assembly, and the liquid injection needle The sealing assembly includes: a sealing column arranged on the cover plate of the measuring chamber, a sealing column cover plate arranged on the top of the sealing column, a first sealing plug connected to the sealing column cover plate in the groove on the top of the sealing column, and a sealing column arranged on the top of the sealing column. The first O-ring on the outer periphery of the first sealing plug, the sealing column cover plate, the first sealing plug and the sealing column are formed with a through hole for the injection needle to pass through, and the bottom of the sealing column is provided with a diameter ratio injection needle an accommodating cavity with a large outer diameter; the suction needle sealing assembly includes: a second sealing plug arranged in the groove on the top of the measuring chamber cover and connected to the sealing plug cover arranged on the measuring chamber cover; On the second O-ring on the outer periphery of the second sealing plug, the sealing plug cover plate and the second sealing plug are formed with a through hole through which the suction needle passes.

Preferably, between the liquid injection needle and the first sealing plug, between the liquid suction needle and the second sealing plug, between the first O-ring and the first sealing plug, and between the second O-ring and the second sealing plug Both use an interference fit.

Preferably, the inner holes of the first sealing plug and the second sealing plug are tapered hole structures, and the first sealing plug and the second sealing plug are preferably wear-resistant black plastic.

Preferably, it consists of a top plate, a vertical plate and a base, the base is fixed on the cover plate of the measuring chamber, and the linear guide rail is arranged on the vertical plate.

Preferably, the upper and lower ends of the vertical plate are provided with two limit pins for limiting the position of the slider.

Preferably, an optocoupler baffle is installed on the drive base, and an optocoupler corresponding to the optocoupler baffle is installed on the vertical plate; before the turntable rotates, the optocoupler baffle and the optocoupler are sensed to communicate to the control system. Send a signal to reset the entire needle assembly.

Preferably, the suction needle is connected with the waste liquid pipe, and the waste liquid pipe is connected with the waste liquid pump; the liquid injection needle is connected with the liquid injection pipe, and the liquid injection pipe is connected with the liquid injection pump.

Preferably, a notch is provided at the tip of the pipetting needle.

Preferably, the injection needle has a tapered tip.

The beneficial effects of the present utility model are:

The measuring chamber liquid injection and liquid suction device of the utility model can make the reaction cup in the reaction cup accommodation position pass through the liquid injection level and the liquid suction level in sequence, that is, when the reaction cup rotates to the bottom of the liquid injection needle, it is at the liquid injection level, When the cuvette is turned to the bottom of the suction needle, it is at the suction position. In the continuous test, when the former cuvette is at the waste liquid suction position, the latter cuvette is at the injection position, that is, suction waste liquid and injection buffer (excitation This greatly saves the test time; especially the liquid injection and liquid suction device has both liquid injection and liquid suction functions. After the injection of buffer (exciter) to complete the luminescence measurement, the reaction cup can be transferred to the bottom. One station (liquid suction position), to directly discharge the waste liquid of the reaction cup to the outside of the system for centralized recovery and treatment, and then transfer the reaction cup to the next station (reaction cup pick-and-place position) for the reaction without waste liquid The cup is taken away to realize the dry and wet separation of waste liquid and the used reaction cup, and the degree of automation is extremely high.

Description of drawings

The technical solutions of the present application will be further described below with reference to the accompanying drawings and embodiments.

Fig. 1, Fig. 2 are the structural schematic diagrams of the liquid injection and liquid suction device of the measuring chamber according to the embodiment of the present application;

[Corrected 11.06.2021 according to Rule 26]
FIG. 3 is a cross-sectional view of a liquid filling and liquid suction device for a measurement chamber according to an embodiment of the present application.

The reference numbers in the figure are: 1- bracket, 11- top plate, 12- vertical plate, 13- base, 2- drive assembly, 21- screw motor, 23- drive base, 24- optocoupler baffle, 25- Optocoupler, 26-slider, 27-limiting pin, 28-drive screw, 29-screw nut, 3-pin assembly, 31-pin fixing plate, 32-liquid aspiration needle, 33-liquid injection needle, 4 -Injection needle seal assembly, 41-First O-ring, 42-First seal plug, 43-Seal column cover, 44-Seal column, 5-Liquid aspiration needle seal assembly, 51-Seal plug cover, 52 -Second sealing plug, 53-Second O-ring, 6-Cuvette, 7-Turntable, 8-Measuring chamber cover.

Detailed ways

It should be noted that the embodiments in the present application and the features of the embodiments may be combined with each other in the case of no conflict.

In the description of this application, it should be understood that the terms "center", "portrait", "horizontal", "top", "bottom", "front", "rear", "left", "right", " The orientations or positional relationships indicated by vertical, horizontal, top, bottom, inner, and outer are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and The description is simplified rather than indicating or implying that the referred device or element must have a specific orientation, be constructed and operate in a specific orientation, and therefore should not be construed as limiting the scope of protection of the present application. In addition, the terms "first", "second", etc. are used for descriptive purposes only, and should not be construed as indicating or implying relative importance or implying the number of indicated technical features. Thus, a feature defined as "first", "second", etc., may expressly or implicitly include one or more of that feature. In the description of the present invention, unless otherwise specified, the meaning of "plurality" is two or more.

In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it may be a fixed connection or a detachable connection Connection, or integral connection; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through an intermediate medium, can be internal communication between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood through specific situations.

The technical solutions of the present application will be described in detail below with reference to the accompanying drawings and in conjunction with the embodiments.

Example

This embodiment provides a liquid injection and liquid suction device in a measurement chamber, as shown in Figures 1, 2, and 3, including a bracket 1, a drive assembly 2, a needle assembly 3, a liquid injection needle sealing assembly 4 and a liquid suction needle sealing assembly 5;

The bracket 1 is composed of a top plate 11, a vertical plate 12, and a base 13. The base 13 is fixed on the measuring chamber cover plate 8 on the top of the measuring chamber. The measuring chamber is provided with a turntable 7, and the turntable 7 is provided with a plurality of edge turntables 7. The circumferentially arranged cuvette container for accommodating the cuvette 6 is positioned, and the turntable 7 is driven to rotate by the driving member so that the cuvette 6 in the cuvette container position can pass through the liquid injection level and the liquid suction level successively;

The needle assembly 3 includes a suction needle 32, a liquid injection needle 33, and a needle fixing plate 31 located on the upper part of the measuring chamber cover 8, which is fixedly connected with the liquid suction needle 32 and the liquid injection needle 33. The liquid needles 33 respectively pass through the cover plate 8 of the measuring chamber and correspond to the cuvette 6 on the liquid suction level and the liquid injection level. The needle tip of the liquid suction needle 32 and the liquid injection needle 33 have a certain height difference; further, the liquid suction needle There is a notch at the tip of the needle 32, which can prevent the needle opening of the aspiration needle 32 from being blocked when the bottom of the reaction cup 6 is attached.

The driving assembly 2 includes a screw motor 21 , a driving screw 28 , a screw nut 29 that is screwed on the driving screw 28 and is fastened to the needle fixing plate 31 , and a driver that is fixed to the needle fixing plate 31 . The seat 23, the linear guide rail arranged on the vertical plate 12 in parallel with the drive screw 28, and the slider 26 fixed on the drive base 23 and matched with the linear guide rail, one end of the drive screw 28 is connected to the output shaft of the screw motor 21, The other end is connected with the measuring chamber cover plate 8 through the bearing 22. The liquid suction needle 32 and the liquid injection needle 33 fixed on the needle fixing plate 31 can be moved up and down through the screw motor 21 and the driving screw 28, and the needle fixing plate can pass through the needle fixing plate. 31. The driving seat 23 drives the sliding block 26 to move up and down along the linear guide rail, so as to ensure that the suction needle 32 and the liquid injection needle 33 run smoothly up and down;

The liquid injection needle sealing assembly 4 and the liquid suction needle sealing assembly 5 are used for sealing when the liquid injection needle 33 and the liquid suction needle 32 move up and down to prevent the entry of external light. 4 includes: a sealing column 44 arranged on the cover plate 8 of the measuring chamber, a sealing column cover 43 arranged on the top of the sealing column 44, and a first seal connected to the sealing column cover 43 in the groove at the top of the sealing column 44 The plug 42, and the first O-ring 41 arranged on the outer periphery of the first sealing plug 42, the sealing column cover plate 43, the first sealing plug 42, and the sealing column 44 are formed with a through hole for the liquid injection needle 33 to pass through, The bottom of the sealing column 44 is provided with an accommodating cavity whose diameter is larger than the outer diameter of the liquid injection needle; the liquid suction needle sealing assembly 5 includes: a groove arranged in the top groove of the cover plate 8 of the measuring chamber and a cavity disposed on the cover plate 8 of the measuring chamber The second sealing plug 52 connected to the upper sealing plug cover 51, and the second O-ring 53 arranged on the outer circumference of the second sealing plug 52, the sealing plug cover 51 and the second sealing plug 52 are formed with suction The through hole through which the liquid needle 32 passes; between the liquid injection needle 33 and the first sealing plug 42, between the liquid suction needle 32 and the second sealing plug 52, between the first O-ring 41 and the first sealing plug 42, Interference fit is used between the second O-ring 53 and the second sealing plug 52 , the inner holes of the first sealing plug 42 and the second sealing plug 52 adopt a tapered hole structure, and the first sealing plug 42 and the second sealing plug 52 Further use of wear-resistant black plastics, such as black PTFE, black high-hardness PU, black UPE, etc., can effectively prevent external light from entering the internal chamber of the measuring chamber. In addition, the reason why the liquid injection needle sealing assembly 4 has more design of the sealing column 44 than the liquid suction needle assembly 5 is mainly because the needle tip of the liquid suction needle 32 and the needle tip of the liquid injection needle 33 have a certain height difference. When the home position is reached, if there is no sealing column 44 and the liquid injection needle sealing assembly is provided at the cover plate 8 of the measuring chamber, the liquid injection needle 33 and the sealing assembly will be separated.

Further, the upper and lower ends of the vertical plate 12 are provided with two limit pins 27, which are used to limit the slider 26, that is, when the entire mechanism fails, the suction needle 32 and the liquid injection needle 33 will not be caused. Run up or down without limit to avoid malfunction, such as piercing the cuvette 6;

Further, an optocoupler block 24 is installed on the drive base 23, and an optocoupler 25 corresponding to the optocoupler block 24 is installed on the vertical plate 12; 25, so as to send a signal to the control system to realize the reset of the entire needle assembly 3; when the needle assembly 3 is in the home position, the suction needle 32 and the liquid injection needle 33 are located above the cup hole of the corresponding cuvette 6 (greater than mm) In order to prevent the needle tip from interfering with the rotation of the cuvette 6 and cause a malfunction, the needle tips of the liquid suction needle 32 and the liquid injection needle 33 cannot be separated from the inner hole of the sealing plug 42, so as to prevent the liquid suction needle 32 and the liquid injection needle 33 from running downward again. Align the inner hole of the sealing plug 42;

Further, the suction needle 32 is connected with the waste liquid pipe, and the waste liquid pipe is connected with the waste liquid pump; the liquid injection needle 33 is connected with the liquid injection pipe, and the liquid injection pipe is connected with the liquid injection pump; , the liquid suction needle 32 and the liquid injection needle 33 are slowly lowered under the drive of the screw motor 21. During the descending process, the waste liquid pump is always in a working state until the liquid suction needle 32 touches the bottom of the cuvette 6, and when the liquid injection needle 33 The injection pump starts to work when the tip of the needle enters the cuvette 6. In the past, buffer was injected into the cuvette 6. When the suction needle 32 touched the bottom of the cuvette 6, the injection needle 33 could not touch the buffer in the cuvette.

During use, when the cuvette 6 is rotated to the bottom of the liquid injection needle 33, it is at the liquid injection level; when the cuvette 6 is rotated to the bottom of the liquid suction needle 32, it is in the liquid suction position. In the continuous test, the previous cuvette 6 is in the liquid injection position. When the waste liquid level is suctioned, the latter reaction cup 6 is at the liquid injection level, that is, the waste liquid suction and the buffer injection are carried out at the same time, which greatly saves the test time.

Taking the above ideal embodiments according to the present application as inspiration, and through the above descriptions, relevant personnel can make various changes and modifications without departing from the technical idea of the present application. The technical scope of the present application is not limited to the content in the description, and the technical scope must be determined according to the scope of the claims.

Claims

A liquid injection and suction device for a measuring chamber, characterized in that it includes:

The bracket (1) is fixed on the cover plate (8) of the measuring chamber at the top of the measuring chamber, a turntable (7) is arranged in the measuring chamber, and a plurality of rotating disks (7) are arranged along the circumference of the turntable (7) for accommodating The reaction cup container (6) of the reaction cup (6) is positioned, and the turntable (7) is driven to rotate by the driving member, so that the reaction cup (6) in the position of the reaction cup container can pass through the liquid injection level and the liquid suction level successively;

A needle assembly (3), comprising a liquid suction needle (32), a liquid injection needle (33), and a needle fixing needle located on the upper part of the measuring chamber cover plate (8) fixedly connected with the liquid suction needle (32) and the liquid injection needle (33) a plate (31), the liquid suction needle (32) and the liquid injection needle (33) respectively pass through the measuring chamber cover plate (8) and correspond to the reaction cup (6) on the liquid suction level and the liquid injection level;

The drive assembly (2) includes a screw motor (21), a driving screw (28), and a screw nut (29) that is screwed on the driving screw (28) and is fastened to the needle fixing plate (31). ), a drive seat (23) fixed on the needle fixing plate (31), a linear guide rail arranged on the bracket (1) parallel to the drive screw (28), and a linear guide rail fixed on the drive seat (23) that matches the linear guide rail The slider (26), one end of the drive screw (28) is connected to the output shaft of the screw motor (21), the other end is connected to the measuring chamber cover (8) through the bearing (22), and the screw motor (21) , the drive screw (28) can move the suction needle (32) and the liquid injection needle (33) fixed on the needle fixing plate (31) up and down, and drive the needle fixing plate (31) and the driving seat (23) to drive The slider (26) moves up and down along the linear guide.
The liquid injection and liquid suction device according to claim 1, characterized in that, the needle tip of the liquid suction needle (32) and the needle tip of the liquid injection needle (33) have a certain height difference.
The liquid injection and suction device for the measurement chamber according to claim 2, characterized in that, the measurement chamber liquid injection and suction device further comprises a liquid injection needle sealing assembly (4) and a liquid suction needle sealing assembly (5), The liquid injection needle sealing assembly (4) comprises: a sealing column (44) arranged on the measuring chamber cover plate (8), a sealing column cover plate (43) arranged on the top of the sealing column (44), and a sealing column cover plate (43) arranged on the sealing column (44) A first sealing plug (42) in the top groove connected to the sealing column cover plate (43), and a first O-ring (41) arranged on the outer periphery of the first sealing plug (42), the sealing The column cover plate (43), the first sealing plug (42), and the sealing column (44) are formed with a through hole for the liquid injection needle (33) to pass through, and the bottom of the sealing column (44) is provided with a diameter ratio liquid injection needle an accommodating cavity with a large outer diameter; the liquid suction needle sealing assembly (5) comprises: a sealing plug cover plate ( 51) The connected second sealing plug (52), and the second O-ring (53) arranged on the outer periphery of the second sealing plug (52), the sealing plug cover plate (51), the second sealing plug (52) ) is formed with a through hole through which the aspiration needle (32) passes.
The liquid injection and suction device for the measurement chamber according to claim 3, characterized in that, between the liquid injection needle (33) and the first sealing plug (42), and between the liquid suction needle (32) and the second sealing plug (52) ), between the first O-ring (41) and the first sealing plug (42), and between the second O-ring (53) and the second sealing plug (52), use an interference fit.
The liquid injection and suction device for the measurement chamber according to claim 3 or 4, characterized in that, the inner holes of the first sealing plug (42) and the second sealing plug (52) adopt a tapered hole structure, and the first sealing plug (52) adopts a tapered hole structure. The plug (42) and the second sealing plug (52) are preferably made of wear-resistant black plastic.
The liquid filling and liquid suction device for a measuring chamber according to any one of claims 1-5, characterized in that it is composed of a top plate (11), a vertical plate (12), and a base (13), and the base (13) is fixed on the measuring chamber. On the chamber cover plate (101), the linear guide rail is arranged on the vertical plate (12).
The liquid filling and liquid suction device for the measuring chamber according to claim 6, characterized in that, two limit pins (27) are provided on the upper and lower ends of the vertical plate (12) for aligning the slider (26) Limit.
The liquid filling and suction device for a measuring chamber according to any one of claims 1 to 7, wherein an optocoupler blocking plate (24) is installed on the driving seat (23), and the vertical plate (12) An optocoupler (25) corresponding to the optocoupler baffle (24) is installed on it; before the turntable (7) rotates, a signal is sent to the control system through the induction of the optocoupler baffle (24) and the optocoupler (25). Reset of the entire needle assembly (3).
The liquid injection and liquid suction device in the measurement chamber according to any one of claims 1-8, wherein the liquid suction needle (32) is connected to a waste liquid pipe, and the waste liquid pipe is connected to a waste liquid pump; the liquid injection needle (33) Connect with the liquid injection pipe, and the liquid injection pipe is connected with the liquid injection pump.
The liquid injection and liquid suction device for the measurement chamber according to any one of claims 1 to 9, wherein a notch is formed at the tip of the liquid suction needle (32), and the liquid injection needle (33) is preferably Has a tapered tip.