CN115418305A

CN115418305A - High-flux virus enrichment device and method in sewage

Info

Publication number: CN115418305A
Application number: CN202210994378.2A
Authority: CN
Inventors: 余昊翔; 李擎; 边宝丽; 吴佳育; 李丹
Original assignee: Hky Technology Co ltd
Current assignee: Hky Technology Co ltd
Priority date: 2022-08-18
Filing date: 2022-08-18
Publication date: 2022-12-02

Abstract

The invention relates to a high-flux virus enrichment device and method in sewage. The method can effectively reduce the cost of virus enrichment in water, reduce high-frequency contact between experimenters and samples, and realize the purpose of automatically enriching the virus in the water in batches while avoiding cross contamination of the samples. According to the device and the method for high-flux enrichment of the virus in the sewage, provided by the invention, the reagent unit, the sample unit, the reaction unit and the collection unit are arranged, and the 4 channels are adopted to run simultaneously, so that the functions of automatic disinfection, cleaning, sampling, stirring, quick automatic pH value adjustment, reaction, collection, waste liquid discharge and the like are realized, the preparation is made for the step of finally enriching the flocculating constituent, the workload of experimenters can be reduced, and the efficiency is improved. Moreover, the device can continuously process samples, can realize simultaneous reaction of the samples and disinfection of the reaction container, can save waiting time and reduce the frequency of contact of experimental personnel with the samples, thereby reducing the infection risk of operating personnel and reducing environmental pollution.

Description

High-flux virus enrichment device and method in sewage

Technical Field

The invention belongs to the technical field of virus treatment in water, and particularly relates to a high-flux virus enrichment device and method in sewage.

Background

As the urbanization process is accelerated continuously, the population of residents is concentrated continuously, and the problem that water source pollution is the core problem for guaranteeing the safety of drinking water is avoided. Therefore, the rapid and effective detection of the virus in the water body is the first condition for blocking pathogen transmission and reducing disease prevalence.

Because the concentration of viruses in water is often very low and the turbidity difference of different types of water bodies is large, virus detection can be performed only after enrichment and concentration are performed by using different modes, and common enrichment methods comprise flocculation precipitation, an adsorption elution method, an ultrafiltration method and the like. Wherein, the flocculation precipitation method is mainly used for enriching viruses by adjusting the pH value and adding chemical substances into water to form flocculation precipitation and then collecting floccules. The principle is that viruses are adsorbed and surrounded by flocculent substances, the surfaces of the flocculants are provided with certain charges, the viruses have different isoelectric points, and charge attraction exists between the viruses and the flocculants. The adsorption elution method mainly utilizes the adsorption of an electric filter membrane to concentrate viruses, and then changes the electric charge quantity carried on the surface of the viruses by adjusting the pH value or adding multivalent salt ions into a water sample, thereby changing the adsorption strength of an adsorbent and the viruses and eluting the viruses. The ultrafiltration method mainly depends on physical screening, does not need to adjust the pH value of a water sample, does not need to add a cationic substance into the water sample, is only related to the size of virus particles and the aperture of a filter membrane, and is suitable for enriching the virus particles in other relatively clean water samples such as drinking water and the like.

Based on the above method principles, the applied invention patents and products developed on the market are as follows: the Chinese patent application (application No. 202021615922.0, published 2021 year 1 month 1) discloses a sewage collecting device for monitoring virus and bacteria, which has the advantages that continuous sampling can be realized by combining a piston pump and a filter membrane; the disadvantage is that the enrichment membrane enriched in the sampling device may require a second elution and a second enrichment before further detection can be performed. The Chinese patent application (application number 202023077529.3, published 2021 year 10 month 1) discloses a portable water sample concentrated virus enrichment device, which has the advantages that a micro vacuum pump can replace manual thrust to push an injector for enrichment, partial experimental workload can be reduced, and the efficiency can be improved; the method has the defects that the sewage with larger turbidity is enriched, and the phenomenon of filter membrane blockage exists, so that the efficiency is reduced. The Chinese patent application (application number 202110166865.5, published 2021, 5 months and 18 days) discloses a pretreatment device for rapid enrichment of viruses in water, which has the advantages of low treatment cost and high automation degree; the defect is that the single channel is adopted, only one sample can be processed at a time, meanwhile, after one sample is processed, the filter paper in the machine needs to be manually replaced, and then the next operation can be carried out.

At present, equipment developed based on a charge membrane method is also available in the market, and has the advantages of low cost and simplicity and convenience in operation, and the equipment is only suitable for water bodies with low water turbidity, otherwise, the phenomenon of filter membrane blockage is easy to occur. If the device is used for treating high-turbidity sewage, the high-turbidity sewage can be treated by an instrument only by replacing a filter membrane or removing impurities in advance after centrifuging and roughly filtering a sample, but the loss of the target substances enriched in the sample is excessive due to excessive flow steps of the treatment, so that the recovery rate of virus detection can be reduced. The other equipment developed by the adsorption elution method has similar problems (the sewage needs to be pretreated), and the treatment cost is high.

More importantly, in the face of some sudden epidemic outbreaks, an emergency situation that the number of samples is large and the required time is short exists. The virus daily monitoring is carried out on regional environmental water samples, so that the scheme mode of early warning epidemic situations in advance is realized, and the demand of continuous batch enrichment operation on the water samples can also exist. Moreover, batch detection can be realized for the enriched samples at present, which puts a high demand on whether the pretreatment operation of the water sample can rapidly and effectively enrich the viruses in the water in batches.

In addition, in view of pathogenicity of most viruses, how to reduce high-frequency contact between detection personnel and a sample and replace manual operation with instrument operation as much as possible form a simpler, closed and safe enrichment process, and the problem of important consideration in developing a virus enrichment device in water is also solved.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a device and a method for enriching viruses in sewage at high flux, which can improve the efficiency of enriching the viruses in a water body in batches while avoiding sample cross contamination, and can also obviously reduce the water sample enrichment cost, reduce the infection risk of operators and reduce the environmental pollution.

In order to achieve the above purposes, the invention adopts the technical scheme that: a high throughput virus enrichment device in sewage, comprising: the device comprises a device box body, a control unit, a reagent unit, a sample unit, a reaction unit and a collection unit, wherein the control unit, the reagent unit, the sample unit, the reaction unit and the collection unit are arranged in the device box body; the bottom of the box body of the device is provided with a vertical clapboard, and the reagent unit and the sample unit are respectively arranged at two sides of the vertical clapboard; a horizontal partition plate is arranged in the middle of the box body of the device;

the reagent unit comprises a pure water tank, a disinfectant tank, an acid liquid tank, an alkaline liquid tank and a flocculating agent tank;

the device comprises a sample unit, a sample unit and a control unit, wherein the sample unit comprises a sample disc, a water cleaning tank, a disinfectant tank, a first slide rail, a sampling bottle rubber cover, a first XY-axis sliding table and 4 sampling needles, the first slide rail is fixedly arranged on a device box body, the device box body is connected with the sample disc in a sliding mode through the first slide rail, the sample disc comprises a plurality of rows of sampling bottle frames, and 4 sampling bottle holes with specified intervals are arranged on each row of sampling bottle frames and used for placing the sampling bottles; the sample injection needles are arranged on the sample injection needle frame side by side at the specified distance, the first XY axis sliding table is fixedly arranged on the vertical partition plate, and one end of the sample injection needle frame is fixedly connected to the first XY axis sliding table; the pure water tank is connected with the water cleaning tank; the disinfectant tank is connected with the disinfectant tank;

the reaction unit comprises 12 reaction cups, a reaction cup sealing cover, a circular reaction rotary table, a pH meter support, a pH meter and a seventh sliding table, the support of the circular reaction rotary table is fixedly arranged on the horizontal partition plate, the circular reaction rotary table comprises 12 reaction cup holes with the same size, and the circle centers of the 12 reaction cup holes are uniformly distributed on a concentric circle of the circumference of the circular reaction rotary table at equal intervals; the seventh sliding table is fixedly arranged on a back plate of the device box body, and the pH meter bracket is a cross-shaped bracket and is used for supporting and fixing a pH meter electrode; 4 end parts of the cross-shaped bracket are respectively fixedly connected with the top parts of the 4 reaction cup sealing covers, and one end of the pH meter bracket is fixedly connected to the seventh sliding table sliding block; the acid solution tank is connected with acid solution holes on the sealing covers of the four reaction cups through pipelines; the alkaline solution tank is connected with alkaline solution holes on the sealing covers of the four reaction cups through pipelines; the flocculant tank is connected with flocculant holes on the four reaction cup sealing covers through pipelines; each sample injection needle is connected with a sample injection hole on a sealing cover of the reaction cup through a sample injection pipeline;

the collecting unit comprises a collecting disc, a waste liquid groove, a spiral cover centrifuge tube, a spiral cover motor, a second XY-axis sliding table, a third XY-axis sliding table and 4 sample outlet needles, a second sliding rail is fixedly mounted on the device box body, the device box body is connected with the collecting disc in a sliding mode through the second sliding rail, the collecting disc comprises a plurality of rows of centrifuge tube frames, and each row of centrifuge tube frames are provided with 4 centrifuge tube holes at preset intervals and used for containing the spiral cover centrifuge tube; the sample discharging needles are arranged on the sample discharging needle frame side by side at the preset interval, and the cover screwing motors are arranged on the cover screwing motor frame side by side at the preset interval; a second XY-axis sliding table is fixedly arranged on the upper part of the vertical partition plate, and one end of the sample outlet needle frame is fixedly connected to the second XY-axis sliding table; a third XY axis sliding table is fixedly arranged on the device box body, and one end of the cap screwing motor frame is fixedly connected to the third XY axis sliding table; each sample outlet needle is connected with a sample outlet hole on a reaction cup sealing cover through a sample outlet pipeline.

Further, the reagent unit is provided with a first diaphragm pump, a second diaphragm pump, a first quantitative injection pump, a second quantitative injection pump, a third quantitative injection pump and a quintuplet valve group;

the pure water tank is connected with the inlet end of the first diaphragm pump through a hose, and the output end of the first diaphragm pump is connected with the water cleaning tank through a hose; the disinfectant tank is connected with the inlet end of the second diaphragm pump through a hose, and the output end of the second diaphragm pump is connected with the disinfectant tank through a hose;

the acid liquid tank is connected with a first quantitative injection pump through a pipeline controlled by an input valve of a first quintuplet valve group, and four outlet pipelines respectively controlled by four output valves of the first quintuplet valve group respectively penetrate through acid liquid holes on sealing covers of four reaction cups; the alkali liquor tank is connected with a second quantitative injection pump through a pipeline controlled by an input valve of a second quintuplet valve group, and four outlet pipelines respectively controlled by four output valves of the second quintuplet valve group respectively penetrate through alkali liquor holes on sealing covers of four reaction cups; the flocculant tank is connected with a third quantitative injection pump through a pipeline controlled by an input valve of a third quintuplet valve group, and four outlet pipelines respectively controlled by four output valves of the third quintuplet valve group respectively penetrate through flocculant holes on four reaction cup sealing covers;

the acid liquor tank is used for storing hydrochloric acid; the alkali liquor tank is used for storing sodium hydroxide; the flocculant tank is used for storing flocculant, and the flocculant is aluminum chloride;

the cover screwing motor, a power switch of the pH meter, a quintuplet valve group power supply, a first diaphragm pump power supply, a second diaphragm pump power supply, a first quantitative injection pump power supply, a second quantitative injection pump power supply and a third quantitative injection pump power supply are respectively and electrically connected with the control unit.

Further, liquid level sensors are arranged in the pure water tank, the disinfectant tank, the acid liquor tank, the alkali liquor tank and the flocculating agent tank;

the liquid level sensor power supply is electrically connected with the control unit.

Further, the designated interval is the central distance between adjacent sampling bottle holes in each row of sampling bottle racks;

the outlet end of each sample injection needle is respectively connected with the input end of a sample injection pipeline, each sample injection pipeline is respectively clamped in one channel of the first 4-channel peristaltic pump, and the output end of each sample injection pipeline penetrates through a sample injection hole on a reaction cup sealing cover;

the preset distance is the central distance between adjacent centrifuge tube holes in each row of centrifuge tube racks;

the inlet end of each sample outlet needle is respectively connected with the output end of a sample outlet pipeline, each sample outlet pipeline is respectively clamped in one channel of the second 4-channel peristaltic pump, and the input end of each sample outlet pipeline respectively penetrates through a sample outlet hole in a reaction cup sealing cover;

the first 4-channel peristaltic pump and the second 4-channel peristaltic pump are respectively electrically connected with the control unit.

Further, the reaction unit is positioned above the horizontal partition plate, the reagent unit is positioned below the horizontal partition plate, and the collection unit is positioned above the sample unit;

the sample tray comprises 4 rows of sampling bottle racks, and the collection tray comprises 4 rows of centrifuge tube racks;

ultraviolet lamps are arranged above the sample disc, above the collecting disc and on the circular reaction rotating disc;

the control unit is electrically connected with the ultraviolet lamp power switch.

Further, the first XY axis sliding table comprises a third sliding table and a fourth sliding table, the fourth sliding table is fixedly arranged on the vertical partition plate, the third sliding table is fixedly arranged on a fourth sliding table sliding block, and the right end of the sample injection needle frame is fixedly connected to the third sliding table sliding block;

the third sliding table and the fourth sliding table are both provided with a groove photoelectric sensor;

the second XY axis sliding table comprises a first sliding table and a second sliding table, the second sliding table is fixedly arranged at the upper part of the vertical partition plate, the first sliding table is fixedly arranged on a sliding block of the second sliding table, and the right end of the sample outlet needle frame is fixedly connected to the sliding block of the first sliding table;

the first sliding table and the second sliding table are both provided with a groove photoelectric sensor;

the third XY-axis sliding table comprises a fifth sliding table and a sixth sliding table, the sixth sliding table is fixedly arranged on the device box body, the fifth sliding table is fixedly arranged on a sixth sliding table sliding block, and the left end of the cover screwing motor frame is fixedly connected to the fifth sliding table sliding block;

the fifth sliding table and the sixth sliding table are both provided with a groove photoelectric sensor;

a groove photoelectric sensor is arranged on the seventh sliding table;

the control unit is electrically connected with the first sliding table motor, the second sliding table motor, the third sliding table motor, the fourth sliding table motor, the seventh sliding table motor, the fifth sliding table motor and the sixth sliding table motor respectively.

Further, the reaction unit also comprises a reaction turntable motor, and the reaction turntable motor is arranged below the circular reaction turntable;

the pH meter electrode sequentially penetrates through the pH meter bracket and the pH meter hole on the reaction cup sealing cover;

the reaction cups comprise a first reaction cup, a second reaction cup, a third reaction cup, a fourth reaction cup, a fifth reaction cup, a sixth reaction cup, a seventh reaction cup, an eighth reaction cup, a ninth reaction cup, a tenth reaction cup, an eleventh reaction cup and a twelfth reaction cup, and are sequentially placed in 12 reaction cup holes clockwise;

the control unit is electrically connected with the circular reaction turntable motor.

Further, the reaction unit also comprises a magnetic stirrer and a magnetic stirrer, wherein the magnetic stirrer is placed inside the reaction cup; the magnetic stirrer is arranged on the bracket of the round reaction turntable and is positioned right below the reaction cup;

the magnetic stirrer is electrically connected with the control unit;

the bottom of the circular reaction turntable is provided with universal wheels.

The invention also provides a high-throughput enrichment method of viruses in sewage, which comprises the following steps:

s1, preparing a sample and a screw cap centrifuge tube: respectively loading samples into a first batch of sampling bottles, a second batch of sampling bottles, a third batch of sampling bottles and a fourth batch of sampling bottles, and placing the first batch of sampling bottles, the second batch of sampling bottles, the third batch of sampling bottles and the fourth batch of sampling bottles on 4 rows of sampling bottle racks of a sample tray;

respectively placing a first batch of centrifuge tubes, a second batch of centrifuge tubes, a third batch of centrifuge tubes and a fourth batch of centrifuge tubes on 4 rows of centrifuge tube racks of a collecting tray;

s2, disinfecting and cleaning: the sample injection needle is driven to enter the water washing tank through the first XY axis sliding table, and the sample injection needle, the sample injection pipeline, the first reaction cup, the fourth reaction cup, the seventh reaction cup, the tenth reaction cup, the sample outlet pipeline and the sample outlet needle are washed; then the sample injection needle is driven by the first XY axis sliding table to enter the disinfectant tank, the sample injection needle, the sample injection pipeline, the first reaction cup, the fourth reaction cup, the seventh reaction cup, the tenth reaction cup, the sample outlet pipeline and the sample outlet needle are disinfected, and the cleaned and disinfected waste liquid is discharged to a waste liquid tank through the sample outlet pipeline and the sample outlet needle;

s3, sample injection reaction: driving a sample injection needle to extend into the bottom of a first batch of sampling bottles for sampling through a first XY axis sliding table, driving a pH meter electrode to move downwards to a fourth up-and-down moving terminal point through a seventh sliding table motor, and injecting a first batch of samples into a first reaction cup, a fourth reaction cup, a seventh reaction cup and a tenth reaction cup through the sample injection needle and a sample injection pipeline;

respectively injecting aluminum chloride into the first reaction cup, the fourth reaction cup, the seventh reaction cup and the tenth reaction cup, stirring for 2min, and reacting to generate a flocculating constituent if the pH value of the sample measured by the pH meter is within an acceptable pH value range of the sample; if the pH value of the sample measured by the pH meter is not within the acceptable pH value range of the sample, adjusting the pH value of the sample until the pH value of the sample is within the acceptable pH value range of the sample, and reacting to generate a flocculating constituent;

s4, transfer and collection: when the reaction of the first batch of samples in the first reaction cup, the fourth reaction cup, the seventh reaction cup and the tenth reaction cup is finished, the third XY axis sliding table drives the screw cap motor to unscrew the screw cap of the first batch of centrifuge tubes and picks up the screw cap of the first batch of centrifuge tubes to return to the default starting position of the screw cap motor;

under the drive of the second XY axis sliding table, the outlet end of the sample outlet needle extends into the first batch of centrifuge tubes for sample outlet; respectively transferring and collecting reaction liquid in the first reaction cup, the fourth reaction cup, the seventh reaction cup and the tenth reaction cup into four first centrifuge tubes through sample outlet pipelines by 4 sample outlet needles, thereby realizing the sample outlet of the first reaction liquid in the first reaction cup, the fourth reaction cup, the seventh reaction cup and the tenth reaction cup; after the reaction liquid is transferred, the sample outlet needle returns to the default initial position of the sample outlet needle under the drive of the second XY axis sliding table;

then, driving a screw cap motor to screw the screw caps of the first centrifuge tubes on the first centrifuge tubes by a third XY axis sliding table, and driving the screw cap motor to return to the default starting position of the screw cap motor by the third XY axis sliding table;

s5, disinfection and cleaning after reaction: repeating the disinfection and cleaning process of the step S2; then a seventh sliding table motor drives a pH meter electrode to move upwards to a third up-down moving original point, a circular reaction turntable rotates anticlockwise for 30 degrees, at the moment, four reaction cup sealing covers are respectively positioned right above a second reaction cup, a fifth reaction cup, an eighth reaction cup and an eleventh reaction cup, the seventh sliding table motor drives the pH meter electrode to move downwards to a third up-down moving terminal point, a sample injection needle is driven to enter a water cleaning tank through a first XY axis sliding table, pure water is injected into the second reaction cup, the fifth reaction cup, the eighth reaction cup and the eleventh reaction cup through the sample injection needle and a sample injection pipeline, secondary cleaning of the sample injection needle, the sample injection pipeline, a sample outlet pipeline and a sample outlet needle is realized, and cleaned waste liquid is discharged to a waste liquid tank through the sample outlet pipeline and the sample outlet needle;

s6, switching reaction cups, repeating the steps S2 to S5, and sequentially processing samples of different batches: the seventh sliding table motor drives the pH meter electrode to move upwards to a third up-and-down moving original point, the circular reaction turntable rotates anticlockwise by 30 degrees, and at the moment, the four reaction cup sealing covers are respectively positioned right above a third reaction cup, a sixth reaction cup, a ninth reaction cup and a twelfth reaction cup; and controlling the first XY axis sliding table, the second XY axis sliding table and the third XY axis sliding table by the control unit to repeat the processes of sterilization cleaning, sample injection reaction, transfer collection and sterilization cleaning after reaction in the steps S2-S5, and sequentially processing a second batch of samples in a second batch of sampling bottles, a third batch of samples in a third batch of sampling bottles and a fourth batch of samples in a fourth batch of sampling bottles.

Further, the method for adjusting the pH of the sample in step S3 includes the following steps:

s31, judging the pH value adjusting mode of the sample: if the pH value of the sample is within the coarse adjustment interval, the step S32 is carried out; if the pH value of the sample is within the middle adjustment range, the step S33 is executed; if the pH value of the sample is within the fine adjustment interval, the step S34 is executed;

s32, coarse adjustment of pH value: if the pH value of the sample is in a low coarse adjustment interval in the coarse adjustment interval, adding sodium hydroxide with a set coarse adjustment volume; if the pH value of the sample is in a high coarse adjustment interval in a coarse adjustment interval, adding hydrochloric acid with a set coarse adjustment volume; then stirring for 30s, reading the stable pH value of the sample after coarse adjustment by a pH meter 15, reacting to generate a flocculating constituent if the pH value of the sample after coarse adjustment is within an acceptable pH value range of the sample, otherwise, returning to the step S31;

s33, adjusting the pH value: if the pH value of the sample is in a low-medium regulation interval in a medium regulation interval, adding sodium hydroxide with a set medium regulation volume; if the pH value of the sample is in a high-medium regulation interval in the medium regulation interval, adding hydrochloric acid with a set medium regulation volume; then stirring 30s, reading the stable pH value of the sample after the neutralization by a pH meter 15, reacting to generate a flocculating constituent if the pH value of the sample after the neutralization is within an acceptable pH value range of the sample, and returning to the step S31 if the pH value of the sample after the neutralization is not within the acceptable pH value range of the sample;

s34, fine adjustment of pH value: if the pH value of the sample is in a low fine adjustment interval in the fine adjustment interval, adding sodium hydroxide with a set fine adjustment volume; if the pH value of the sample is in a high fine adjustment interval in the fine adjustment interval, adding hydrochloric acid with a set fine adjustment volume; and then stirring 30s, reading the stable pH value of the sample after fine adjustment by the pH meter 15, and returning to the step S31 if the pH value of the sample after fine adjustment is within the acceptable pH value range of the sample.

The invention has the beneficial effects that: by adopting the device and the method for enriching the virus in the sewage at high flux, the cost for enriching the virus in the water can be effectively reduced, and the high-frequency contact between experimenters and samples is reduced; the invention realizes the purpose of automatically enriching viruses in water in batches while avoiding sample cross contamination. According to the device and the method for high-flux enrichment of the viruses in the sewage, provided by the invention, the reagent unit, the sample unit, the reaction unit and the collection unit are arranged, and the 4 channels are adopted to run simultaneously, so that the functions of automatic disinfection, cleaning, sampling, stirring, quick automatic pH value adjustment, reaction, collection, waste liquid discharge and the like are realized, the preparation is made for the step of finally enriching the flocculating constituent, the workload of experimenters can be reduced, and the efficiency of enriching the viruses in the water body in batches is improved. Moreover, the device and the method provided by the invention can continuously process samples, can realize simultaneous sample reaction and reaction container disinfection, and can save waiting time. When the device and the method provided by the invention are applied to the whole reaction process, the sampling bottle rubber cover and the reaction cup sealing cover are used for preventing liquid from splashing and aerosol from volatilizing, the screw cover is screwed off or screwed down through the screw cover motor to reduce the high-frequency contact/operation of an experimenter, and meanwhile, the irradiation killing effect of an ultraviolet lamp is combined, so that the phenomenon of mutual cross contamination among multiple samples can be effectively avoided, the infection risk of the operator can be reduced, and the environmental pollution can be reduced.

Drawings

FIG. 1 is a top view of a structure of a device for high flux enrichment of viruses in wastewater according to an embodiment of the present invention;

FIG. 2 is a front view of a device for high throughput enrichment of viruses in wastewater according to an embodiment of the present invention;

FIG. 3 is a side view of the structure of a device for high flux enrichment of viruses in wastewater according to an embodiment of the present invention;

FIG. 4 is a rear view of the structure of a device for high throughput enrichment of viruses in wastewater according to an embodiment of the present invention;

FIG. 5 is a top view of a circular reaction disk according to an embodiment of the present invention;

FIG. 6 is a front view of a circular reaction carousel according to an embodiment of the present invention;

FIG. 7 is a side view of a sample disk according to an embodiment of the present invention;

FIG. 8 is a side view of a collection tray according to an embodiment of the present invention;

wherein, 1-device box body; 2-a sample injection needle; 3-a sample outlet needle; 4-a collection tray; 5-spiral cover centrifuge tube; 6-sample tray; 7-sampling bottle; 8-a first slipway; 9-a second sliding table; 10-a third slipway; 11-a fourth ramp; 12-a fifth ramp; 13-sixth slip table; 14-a seventh slip table; 15-pH meter; 16-a reaction cup; 17-sealing the reaction cup; 18-round reaction carousel; 19-an ultraviolet lamp; 20-acid liquor tank; 21-lye tank; 22-a flocculant tank; 23-a pure water tank; 24-disinfectant tank; 25-a magnetic stirrer; 26-a first 4-channel peristaltic pump; 27-a second 4-channel peristaltic pump; 28-a quintuplet valve bank; 29-a waste liquid conduit; 30-a control unit; 31-a first diaphragm pump; 32-a second diaphragm pump; 33-a first volumetric infusion pump; 34-a second dosing pump; 35-a third dosing pump; 36-a liquid level sensor; 401-a second sliding track; 402-a waste solution tank; 501-a cap screwing motor; 502-first batch of centrifuge tubes; 503-second batch of centrifuge tubes; 504-third batch of centrifuge tubes; 505-fourth batch of centrifuge tubes; 601-a water cleaning tank; 602-a disinfectant tank; 603-a first slide rail; 701-sampling bottle rubber cover; 702-a first batch of sample bottles; 703-a second batch of sampling bottles; 704-a third batch of sample bottles; 705-a fourth batch of sample bottles; 1501-a pH meter support; 1601-a first reaction cup; 1602-a second reaction cup; 1603-third reaction cup; 1604-fourth reaction cup; 1605-fifth reaction cup; 1606-sixth reaction cup; 1607-seventh reaction cup; 1608-eighth reaction cup; 1609-ninth reaction cup; 1610 to tenth reaction cup 10; 1611-eleventh reaction cup; 1612-twelfth reaction cup; 1701-acid well; 1702-alkaline well; 1703-flocculant pores; 1704-sample injection hole; 1705-sample outlet; 1801-universal wheel; 1802-magnetic stirrer; 1803-reaction turntable motor.

Detailed Description

In order to make those skilled in the art better understand the technical solution of the present invention, the technical solution in the embodiments of the present invention will be further clearly and completely described below with reference to the drawings in the examples of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments.

It should be noted that in the description of the embodiments of the present invention, the terms "lateral", "longitudinal", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

As shown in fig. 1 to 8, the embodiment of the present invention provides a device for high throughput enrichment of viruses in wastewater, the device comprising: the device comprises a device box body 1, a control unit 30, a reagent unit, a sample unit, a reaction unit and a collection unit, wherein the control unit 30, the reagent unit, the sample unit, the reaction unit and the collection unit are arranged in the device box body 1.

The device comprises a device box body 1, a reagent unit, a sample unit and a control unit, wherein the device box body 1 is of a cuboid structure, a vertical partition plate is arranged at the bottom of the device box body 1, and the reagent unit and the sample unit are respectively arranged on two sides of the vertical partition plate; a horizontal partition plate is arranged in the middle of the box body of the device, the reaction unit is positioned above the horizontal partition plate, and the reagent unit is positioned below the horizontal partition plate; the collection unit is located above the sample unit, the control unit 30 is arranged above the collection unit, and the control unit 30 is connected with an external circuit through a lead.

(1) Reagent unit: mainly comprises a pure water barrel 23, a disinfectant tank 24, an acid liquor tank 20, an alkaline liquor tank 21 and a flocculating agent tank 22;

the reagent unit is provided with: a first diaphragm pump 31, a second diaphragm pump 32, a first constant volume syringe pump 33, a second constant volume syringe pump 34, and a third constant volume syringe pump 35; a first 4-channel peristaltic pump 26, a second 4-channel peristaltic pump 27;

wherein the inlet end of the first diaphragm pump 31 is connected to the pure water tank 23 through a hose, the outlet end of the first diaphragm pump 31 is connected to the water cleaning tank 601 through a hose, and pure water in the pure water tank 23 can be added to the water cleaning tank 601 through the first diaphragm pump 31; the inlet end of the second diaphragm pump 32 is connected to the disinfectant tank 24 via a hose, and the outlet end of the second diaphragm pump 32 is connected to the disinfectant tank 602 via a hose, and the disinfectant in the disinfectant tank 24 can be added to the disinfectant tank 602 via the second diaphragm pump 32.

The acid solution tank 20 is used for storing hydrochloric acid; the lye tank 21 is used for storing sodium hydroxide; the flocculant tank 22 is used for storing flocculant, and the flocculant is aluminum chloride.

The reagent unit is also provided with three quintuplet valve groups 28, a first quantitative injection pump 33 is connected with the acid liquor tank 20 through a pipeline controlled by an input valve of the first quintuplet valve group 28, and four outlet pipelines respectively controlled by four output valves of the first quintuplet valve group 28 penetrate through acid liquor holes 1701 on four reaction cup sealing covers 17; the second quantitative injection pump 34 is connected with the alkali liquor tank 21 through a pipeline controlled by the input valve of the second quintuplet valve group 28, and four outlet pipelines respectively controlled by four output valves of the second quintuplet valve group 28 penetrate through the alkali liquor holes 1702 on the four reaction cup sealing covers 17; the third constant-volume injection pump 35 is connected to the flocculant tank 22 through a pipeline controlled by an input valve of the third quintuplet valve group 28, and four outlet pipelines respectively controlled by four output valves of the third quintuplet valve group 28 penetrate through flocculant holes 1703 on four reaction cup sealing covers 17.

The first quantitative injection pump 33 pumps hydrochloric acid from the acid tank 20 through a pipeline controlled by an input valve of the first quintuplet valve group 28, and then sequentially injects the hydrochloric acid into the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 through pipelines respectively controlled by four output valves of the first quintuplet valve group 28 (outlet ends of the pipelines pass through the acid hole 1701), or sequentially injects the hydrochloric acid into the third reaction cup 1603, the sixth reaction cup 1606, the ninth reaction cup 1609 and the twelfth reaction cup 1612. Similarly, the second constant-volume injection pump 34 pumps sodium hydroxide from the lye tank 21 through the pipeline controlled by the input valve of the second five-way valve set 28, and then injects the sodium hydroxide into the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 in sequence or injects the sodium hydroxide into the third reaction cup 1603, the sixth reaction cup 1606, the ninth reaction cup 1609 and the twelfth reaction cup 1612 in sequence through the pipelines respectively controlled by the four output valves of the second five-way valve set 28 (the outlet ends of the pipelines pass through the lye hole 1702). The third constant-volume injection pump 35 pumps the aluminum chloride from the flocculant tank 22 through a pipeline controlled by an input valve of the third quintuplet valve group 28, and then sequentially injects the aluminum chloride into the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 or sequentially injects the aluminum chloride into the third reaction cup 1603, the sixth reaction cup 1606, the ninth reaction cup 1609 and the twelfth reaction cup 1612 through pipelines respectively controlled by four output valves of the third quintuplet valve group 28 (outlet ends of the pipelines pass through flocculant holes 1703).

Optionally, level sensor 36 is all installed in pure water bucket 23, disinfectant jar 24, acidizing fluid jar 20, lye tank 21, the flocculating agent jar 22 for the low liquid level early warning of suggestion reagent, when pure water bucket 23, disinfectant jar 24, acidizing fluid jar 20, lye tank 21, flocculating agent jar 22 in the reagent is not enough, level sensor 36 can send the early warning, in order to remind in advance in time to supply the medicament, avoid appearing because of the not enough enrichment failure phenomenon that leads to of medicament, avoid causing the waste to rare sample.

Optionally, the first 4-channel peristaltic pump 26, the second 4-channel peristaltic pump 27, the quintuplet valve set 28, the first diaphragm pump 31, the second diaphragm pump 32, the first quantitative injection pump 33, the second quantitative injection pump 34, the third quantitative injection pump 35, and the liquid level sensor 36 are all electrically connected to the control unit 30.

(2) Sample unit: the device mainly comprises a sample tray 6, a water cleaning tank 601, a disinfectant cleaning tank 602, a first slide rail 603, a sampling bottle 7 and a sampling bottle rubber cover 701, wherein the sampling bottle 7 comprises a first batch of sampling bottles 702, a second batch of sampling bottles 703, a third batch of sampling bottles 704 and a fourth batch of sampling bottles 705.

As shown in fig. 2, fig. 3 and fig. 7, the device housing 1 is fixedly provided with a first slide rail 603, and the device housing 1 is slidably connected to the sample tray 6 through the first slide rail 603, so as to facilitate taking out or placing the sample bottle 7. The disinfectant cleaning tank 602 and the water cleaning tank 601 are fixedly mounted on the first slide rail 603 and are sequentially arranged behind the sample tray 6. Through setting up first slide rail 603, can conveniently put sampling bottle 7 on sample dish 6, can get sampling bottle 7 alone simultaneously and transfer and place the device in the sterilizer after handling again, can further reduce the pollution of environment and the risk that personnel infected around the experiment.

Optionally, an ultraviolet lamp 19 is mounted above the sample tray 6 to avoid cross contamination of multiple samples while reducing the risk of infection to the operator of the contact device.

As shown in FIG. 7, the sample tray 6 includes 4 rows of sample bottle holders for holding a first batch of sample bottles 702, a second batch of sample bottles 703, a third batch of sample bottles 704 and a fourth batch of sample bottles 705, respectively, wherein each row of sample bottle holders has 4 sample bottle holes at equal intervals for receiving 4 sample bottles of the same batch.

The sample unit also comprises a first XY axis sliding table and 4 sampling needles 2, the sampling needles 2 are arranged on a sampling needle frame side by side at equal intervals, and the interval between every two adjacent sampling needles 2 is the central distance between every two adjacent sampling bottle holes of each row of sampling bottle frames in the sample disc 6; the first XY axis sliding table is fixedly arranged on the vertical partition plate, and one end of the sample injection needle frame is fixedly connected to the first XY axis sliding table.

Specifically, the first XY axle slip table includes third slip table 10, fourth slip table 11, and injection needle frame right-hand member fixed connection is on third slip table 10 slider, and third slip table 10 fixed mounting is on fourth slip table 11 slider, and fourth slip table 11 fixed mounting is on the perpendicular baffle.

First XY axle slip table is used for driving injection needle 2 and carries out around, reciprocates respectively: under the drive of a motor of the third sliding table 10, the rotary motion of a screw of the third sliding table 10 is converted into the upward or downward linear motion of a sliding block of the third sliding table 10, so that a sample injection needle frame is driven to move up and down along the third sliding table 10 (a sample injection needle 2 moves along the Y-axis direction); in a similar way, under the driving of the motor of the fourth sliding table 11, the rotary motion of the screw rod of the fourth sliding table 11 is converted into the forward or backward linear motion of the sliding block of the fourth sliding table 11, so as to drive the sample injection needle holder to move back and forth along the fourth sliding table 11 (the sample injection needle 2 moves along the X-axis direction).

The 4 outlet ends of the sample injection needles 2 are respectively connected with the input ends of the 4 sample injection pipelines, the middle parts of the 4 sample injection pipelines are respectively clamped in the 4 channels of the first 4-channel peristaltic pump 26, and the output ends of the 4 sample injection pipelines penetrate through the sample injection holes 1704 on the reaction cup sealing cover 17.

Optionally, two groove photoelectric sensors are mounted on the third sliding table 10, and a first up-down moving origin and a first up-down moving destination are respectively set for limiting the up-down limit movement position of the movement of the sample injection needle 2; two groove photoelectric sensors are mounted on the fourth sliding table 11, and are respectively set with a first front-back movement origin and a first front-back movement destination, so as to limit the front-back limit movement position of the movement of the sample injection needle 2. Setting the right end of the sample injection needle frame as a default starting position of the sample injection needle when the right end of the sample injection needle frame coincides with the first up-down moving original point and the first up-down moving original point, wherein the default starting position of the sample injection needle is positioned right above the water cleaning tank 601; when the sampling needle is at the default starting position, the inlet end of the sampling needle 2 is higher than the rubber cover 701 of the sampling bottle by not less than 2cm. When the right end of the sampling needle frame coincides with the first up-and-down moving origin and the first back-and-forth moving destination, the four sampling needles 2 are located right above the first sampling bottles 702. After sampling of each batch of samples, cleaning and disinfecting of the sample introduction pipeline, cleaning and disinfecting of the reaction cup and cleaning and disinfecting of the sample discharge pipeline are finished, the sample introduction needle 2 returns to the default starting position of the sample introduction needle under the driving of the third sliding table 10 motor and the fourth sliding table 11 motor.

Optionally, the third sliding table 10 motor, the fourth sliding table 11 motor, and the ultraviolet lamp 19 power supply are all electrically connected to the control unit 30, and according to the sampling, cleaning, and disinfecting operation flow and the control logic provided in this embodiment, an industrial design technician in the field can perform industrial automatic control on the sampling, cleaning, and disinfecting related operation processes of the sample unit in the apparatus through a known industrial control process, so that the workload of the operator can be reduced, and the experimental efficiency can be improved.

The working principle of sample introduction is as follows: when a first batch of samples need to be sampled, under the control of the control unit 30, the motor of the fourth slide table 11 drives the sampling needle 2 to move forward 40cm (i.e. a first forward and backward movement end point) from a default starting position of the sampling needle to reach a position right above a first batch of sampling bottles 702, then the motor of the third slide table 10 drives the sampling needle 2 to move downward to a first upward and downward movement end point, and at the moment, an inlet end of the sampling needle 2 penetrates through the rubber cover 701 of the sampling bottles and extends into the bottoms of the first batch of sampling bottles 702 for sampling; meanwhile, the outlet ends of the 4 sample injection needles 2 are respectively connected with the input ends of the 4 sample injection pipelines, and under the action of the first 4-channel peristaltic pump 26, the samples in the 4 first sampling bottles 702 can be respectively injected into the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 through the sample injection pipelines penetrating through the sample injection holes 1704 or respectively injected into the third reaction cup 1603, the sixth reaction cup 1606, the ninth reaction cup 1609 and the twelfth reaction cup 1612 through the sample injection needles 2, so that the samples in the first sampling bottles 704 can be injected.

Similarly, under the control of the control unit 30, the fourth slide table 11 motor drives the sampling needle 2 to move forward 30cm, 20cm and 10cm from the default starting position of the sampling needle respectively, and then the third slide table 10 motor drives the sampling needle 2 to move downward to the up-and-down moving end point, so that the sampling needle 2 can be controlled to penetrate through the sampling bottle rubber cover 701 and extend into the bottoms of the second batch of sampling bottles 703, the third batch of sampling bottles 704 and the fourth batch of sampling bottles 705 to sample the second batch of samples, the third batch of samples or the fourth batch of samples respectively; under the action of the first 4-channel peristaltic pump 26, the samples in the second batch of sampling bottles 703, the third batch of sampling bottles 704 and the fourth batch of sampling bottles 705 can be respectively injected into the reaction cups through the sampling needles 2, so that the sampling function of different batches of samples is realized.

Optionally, under the control of the control unit 30, the motors of the third sliding table 10 and the fourth sliding table 11 respectively drive the sample injection needles 2 to move up and down and back and forth, 4 sample injection needles 2 can enter the water cleaning tank 601 and the disinfectant cleaning tank 602 respectively, and then under the action of the first 4-channel peristaltic pump 26, the liquids in the water cleaning tank 601 and the disinfectant cleaning tank 602 can be sequentially extracted to the sample injection pipeline, the reaction cup 16 and the sample outlet pipeline through the sample injection needles 2, thereby realizing the cleaning and disinfecting functions of the sample injection pipeline, the reaction cup 16 and the sample outlet pipeline.

(3) A reaction unit: the reaction cup 16 mainly comprises a first reaction cup 1601, a second reaction cup 1602, a third reaction cup 1603, a fourth reaction cup 1604, a fifth reaction cup 1605, a sixth reaction cup 1606, a seventh reaction cup 1607, an eighth reaction cup 1608, a ninth reaction cup 1609, a tenth reaction cup 1610, an eleventh reaction cup 1611 and a twelfth reaction cup 1612; the reaction cup sealing cover 17 comprises an acid liquor hole 1701, an alkali liquor hole 1702, a flocculant hole 1703, a sample inlet hole 1704, a sample outlet hole 1705 and a pH meter hole.

As shown in fig. 1, fig. 2, fig. 3, fig. 5 and fig. 6, the circular reaction turntable 18 includes 12 reaction cup holes with the same size, the circle centers of the 12 reaction cup holes are on a concentric circle of the circumference of the circular reaction turntable 18, and the circle centers of the 12 reaction cup holes are uniformly distributed on the concentric circle at equal intervals, and are used for clockwise sequentially placing a first reaction cup 1601, a second reaction cup 1602, a third reaction cup 1603, a fourth reaction cup 1604, a fifth reaction cup 1605, a sixth reaction cup 1606, a seventh reaction cup 1607, an eighth reaction cup 1608, a ninth reaction cup 1609, a tenth reaction cup 1610, an eleventh reaction cup 1611 and a twelfth reaction cup 1612; the second reaction cup 1602, the fifth reaction cup 1605, the eighth reaction cup 1608 and the eleventh reaction cup 1611 are used as cleaning cups, and are specially used for cleaning and soaking the pH electrode, so that the experiment interference factors are reduced, the daily protection of the pH electrode is facilitated, and the rest reaction cups are used as sample reaction containers.

The pH meter bracket 1501 is a cross-shaped bracket which is positioned right above the circular reaction turntable 18 and is used for supporting and fixing the electrode of the pH meter 15; the 4 end parts of the cross-shaped bracket are respectively fixedly connected with the top parts of the 4 reaction cup sealing covers 17, and are correspondingly provided with corresponding holes with the acid liquor hole 1701, the alkali liquor hole 1702, the flocculating agent hole 1703, the sample inlet hole 1704 and the sample outlet hole 1705 so as to facilitate the passing of pipelines for conveying pure water, disinfectants, hydrochloric acid, sodium hydroxide and aluminum chloride; the electrode of the pH meter 15 sequentially passes through the pH meter bracket 1501 and the pH meter hole on the sealing cover 17 of the reaction cup.

The reaction unit further comprises a seventh sliding table 14 fixedly mounted on a back plate of the device case 1, one end of the pH meter support 1501 is fixedly connected to a slider of the seventh sliding table 14, and four reaction cup sealing covers 17 are respectively positioned right above the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 before sample introduction.

The bracket of the circular reaction turntable 18 is fixedly connected to a horizontal clapboard of the device box body 1; the reaction turntable motor 1803 is installed below the center of the circular reaction turntable 18 and used for driving the circular reaction turntable 18 to rotate so as to switch different reaction cups, so that the reaction of different batches of samples can be realized, and the functions of cleaning the reaction cups, the pH meter 15 electrodes and the sample outlet pipeline can be realized.

Optionally, the bottom of the circular reaction turntable 18 is provided with a universal wheel 1801, which plays a role in supporting and matching the circular reaction turntable 18 to change the direction.

Optionally, two groove photoelectric sensors are mounted on the seventh sliding table 14, and a third up-down moving origin and a third up-down moving destination are set respectively, so as to limit the up-down limit movement position of the electrode of the pH meter 15. When the seventh sliding table 14 sliding block is positioned at the third up-down moving original point, the default starting position of the electrode of the pH meter 15 is set; when the sliding block of the seventh sliding table 14 runs to the end point of the third up-down movement, the bottom of the electrode of the pH meter 15 is positioned at a half height of the reaction cup 16, and at this time, four reaction cup sealing covers 17 cover the four reaction cups 16. Under the drive of a seventh sliding table 14 motor, the rotary motion of a screw of the seventh sliding table 14 is converted into the upward or downward linear motion of a sliding block of the seventh sliding table 14, so that an electrode of a pH meter 15 is driven to move up and down.

Optionally, the reaction unit further includes a magnetic stirrer 1802 and a magnetic stirrer 25, the magnetic stirrer 1802 is placed inside the reaction cup 16, and the magnetic stirrer 25 is mounted on a support of the circular reaction turntable 18 and located right below the reaction cup 16, and is configured to drive the magnetic stirrer 1802, so that the sample in the reaction cup 16 is uniformly stirred. When stirring and reacting are carried out, the reaction cup sealing cover 17 seals the reaction cup 16 which is reacting, so that liquid splashing and aerosol volatilization which possibly occur in the stirring process are avoided, and the phenomenon of cross contamination among samples is reduced. In addition, through the stirring mixing function of magnetic stirrers to the sample, realize accomplishing the flocculating constituent operation of a plurality of quantity samples on a machine, alleviateed operating personnel's work load by a wide margin, promoted experimental efficiency.

Optionally, uv lamps 19 are mounted on the circular reaction carousel 18 to avoid cross contamination of multiple samples while reducing the risk of infection to the operator of the contact device.

Optionally, the seventh sliding table 14 motor, the circular reaction turntable motor 1803, the power switch of the pH meter 15, the power switch of the ultraviolet lamp 19, and the magnetic stirrer 25 are all electrically connected to the control unit 30. According to the operation procedures and control logic of reaction, cleaning, disinfection, and waste liquid discharge provided in the embodiment, industrial design technicians in the field can perform industrial automatic control on the operation processes of reaction, sample discharge, cleaning, disinfection, waste liquid discharge and the like of the reaction unit in the device through a known industrial control procedure, so as to reduce the workload of the operators and improve the experimental efficiency.

The reaction working principle is as follows: if a first batch of samples are input into the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 for reaction, under the control of the control unit 30, the third slide table 10 motor and the fourth slide table 11 motor drive the four sampling needles 2 to extend into the bottom of the first batch of sampling bottles 702 for sampling, the seventh slide table 14 motor drives the pH meter 15 electrode to move downwards to a third up-and-down movement end point, and under the action of the first 4-channel peristaltic pump 26, the first batch of samples are injected into the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 through the sampling needles 2 and the sampling pipes; then, under the control of the quintuplet valve group 28, hydrochloric acid, sodium hydroxide and aluminum chloride are respectively injected into the four reaction cups through the first quantitative injection pump 33, the second quantitative injection pump 34 and the third quantitative injection pump 35, and under the stirring condition provided by the magnetic stirrer 1802 and the magnetic stirrer 25, the pH value of the sample is rapidly adjusted, and a flocculating constituent reaction is generated. After the reaction of the first batch of samples is finished, and the first batch of reaction liquid is sampled, the third sliding table 10 motor and the fourth sliding table 11 motor drive the sample injection needle 2 to enter the water cleaning tank 601, under the action of the first 4-channel peristaltic pump 26, pure water is injected into the four reaction cups through the sample injection needle 2 and the sample injection pipeline, so that the cleaning of the sample injection needle 2, the sample injection pipeline, the four reaction cups, the sample outlet pipeline and the sample outlet needle 3 is realized, and the cleaned waste liquid is discharged to the waste liquid tank 402 through the sample outlet needle 3; and then the third sliding table 10 motor and the fourth sliding table 11 motor drive the sample injection needle 2 to enter the disinfectant cleaning tank 602, under the action of the first 4-channel peristaltic pump 26, a disinfectant is injected into the four reaction cups through the sample injection needle 2 and the sample injection pipeline, so that the sample injection needle 2, the sample injection pipeline, the four reaction cups, the sample outlet pipeline and the sample outlet needle 3 are disinfected, and the disinfected waste liquid is discharged to the waste liquid tank 402.

Then, under the control of the control unit 30, the seventh slide table 14 motor drives the electrode of the pH meter 15 to move upward to the third up-down moving origin, the reaction turntable motor 1803 drives the circular reaction turntable 18 to rotate counterclockwise by 30 °, at this time, the four reaction cup sealing covers 17 are respectively located right above the second reaction cup 1602, the fifth reaction cup 1605, the eighth reaction cup 1608, and the eleventh reaction cup 1611, the seventh slide table 14 motor drives the electrode of the pH meter 15 to move downward to the third up-down moving destination, the third slide table 10 motor and the fourth slide table 11 motor drive the sample injection needle 2 to enter the water cleaning tank 601, and under the action of the first 4-channel peristaltic pump 26, pure water is injected into the four cleaning cups through the sample injection needle 2 and the sample injection pipeline, the sample outlet pipeline, and the sample outlet needle 3, and the cleaned waste liquid is discharged to the waste liquid tank 402 through the sample outlet needle 3.

Then, the seventh sliding table 14 motor drives the electrode of the pH meter 15 to move upwards to the third up-down moving origin, the reaction turntable motor 1803 drives the circular reaction turntable 18 to rotate anticlockwise by 30 °, at this time, the four reaction cup sealing covers 17 are respectively positioned right above the third reaction cup 1603, the sixth reaction cup 1606, the ninth reaction cup 1609 and the twelfth reaction cup 1612, the processes of sample introduction, reaction, sample discharge, cleaning and disinfection are repeated, the second batch of samples in the second batch of sampling bottles 703, the third batch of samples in the third batch of sampling bottles 704 and the fourth batch of samples in the fourth batch of sampling bottles 705 can be sequentially processed, the reaction of different batches of samples is realized, and the time can be greatly shortened.

Optionally, after the first batch of samples are reacted in first reaction cup 1601, fourth reaction cup 1604, seventh reaction cup 1607 and tenth reaction cup 1610, and the samples are taken out and washed, the disinfectant added into first reaction cup 1601, fourth reaction cup 1604, seventh reaction cup 1607 and tenth reaction cup 1610 through sample injection needle 2 may not be discharged, and first reaction cup 1601, fourth reaction cup 1604, seventh reaction cup 1607 and tenth reaction cup 1610 are further soaked for disinfection; the seventh sliding table 14 motor drives the electrode of the pH meter 15 to move up and down, the reaction turntable motor 1803 drives the circular reaction turntable 18 to rotate 30 degrees anticlockwise, so that four reaction cup sealing covers 17 cover the second reaction cup 1602, the fifth reaction cup 1605, the eighth reaction cup 1608 and the eleventh reaction cup 1611 respectively, pure water is injected into the four cleaning cups through the sample injection needle 2 and the sample injection pipeline, secondary cleaning of the sample injection needle 2, the sample injection pipeline, the sample outlet pipeline and the sample outlet needle 3 is realized, and the cleaned waste liquid is discharged to the waste liquid tank 402 through the sample outlet needle 3; then, a seventh sliding table 14 motor drives a pH meter 15 electrode to move up and down, a reaction rotating table motor 1803 drives a circular reaction rotating table 18 to rotate 30 degrees anticlockwise, so that four reaction cup sealing covers 17 are respectively covered on a third reaction cup 1603, a sixth reaction cup 1606, a ninth reaction cup 1609 and a twelfth reaction cup 1612, a sample injection needle 2 is driven by a third sliding table 10 motor and a fourth sliding table 11 motor to extend into the bottom of a second batch of sampling bottles 703 for sampling, a second batch of samples are conveyed to the third reaction cup 1603, the sixth reaction cup 1606, the ninth reaction cup 1609 and the twelfth reaction cup 1612 for reaction, and after the second batch of samples are reacted, discharged and cleaned in the third reaction cup 1603, the sixth reaction cup 1606, the ninth reaction cup 1609 and the twelfth reaction cup 1612, the disinfectant added in the third reaction cup 1603, the sixth reaction cup 1606, the ninth reaction cup 1609 and the twelfth reaction cup 1612 is soaked through the sample injection needle 2; then a seventh sliding table 14 motor drives a pH meter 15 electrode to move up and down, a reaction turntable motor 1803 drives a circular reaction turntable 18 to rotate clockwise for 60 degrees, four reaction cup sealing covers 17 respectively cover the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610, and the disinfected waste liquid is discharged to a waste liquid tank 402 through a sample outlet needle 3; according to the alternative mode, the sample reaction and the reaction container disinfection of different batches are carried out at the same time, so that the waiting time is saved, and the efficiency is improved.

(4) A collection unit: mainly including catch tray 4, spiral cover centrifuging tube 5 includes first batch centrifuging tube 502, second batch centrifuging tube 503, third batch centrifuging tube 504, fourth batch centrifuging tube 505.

As shown in fig. 2, fig. 3 and fig. 8, a second slide rail 401 is fixedly mounted on the apparatus box 1, and the apparatus box 1 is slidably connected to the collecting tray 4 through the second slide rail 401, so as to facilitate taking out or placing the centrifugal tube 5. The waste liquid tank 402 is fixedly mounted on the second slide rail 401, behind the collecting tray 4. Through setting up second slide rail 401, can conveniently put spiral cover centrifuging tube 5 on catch tray 4, can take spiral cover centrifuging tube 5 alone simultaneously and transfer in the sterilizer after handling the device of putting into again, can further reduce the pollution of environment and the risk that personnel infected around the experiment.

Optionally, uv lamp 19 is mounted above collection tray 4 to avoid cross contamination of multiple samples while reducing the risk of infection to the operator of the contact device.

As shown in fig. 8, the collecting tray 4 includes 4 rows of centrifuge tube racks for placing a first batch of centrifuge tubes 502, a second batch of centrifuge tubes 503, a third batch of centrifuge tubes 504, and a fourth batch of centrifuge tubes 505, and each row of centrifuge tube racks is provided with 4 equidistant centrifuge tube holes for accommodating 4 centrifuge tubes of the same batch.

The collecting unit further comprises a second XY-axis sliding table and 4 sample outlet needles 3, the sample outlet needles 3 are arranged on the sample outlet needle frame at equal intervals side by side, the distance between every two adjacent sample outlet needles 3 is the central distance of every row of adjacent centrifuge tube holes of the centrifuge tube rack in the collecting disc 4, the second XY-axis sliding table is fixedly arranged on the upper portion of the vertical partition plate, and one end of the sample outlet needle frame is fixedly connected to the second XY-axis sliding table.

Specifically, the second XY-axis sliding table comprises a first sliding table 8 and a second sliding table 9, the second sliding table 9 is fixedly mounted on the upper portion of the vertical partition plate, the first sliding table 8 is fixedly mounted on a sliding block of the second sliding table 9, and the right end of the sample outlet needle frame is fixedly connected to the sliding block of the first sliding table 8; the second XY axle slip table is used for driving out appearance needle 3 and carries out around, reciprocates: under the drive of a first sliding table 8 motor, the rotary motion of a first sliding table 8 screw is converted into the upward or downward linear motion of a first sliding table 8 slide block, so that a sample outlet needle frame is driven to move up and down along the first sliding table 8 (a sample outlet needle 3 moves along the Y-axis direction); in a similar way, under the driving of the second sliding table 9 motor, the rotary motion of the screw rod of the second sliding table 9 is converted into the forward or backward linear motion of the sliding block of the second sliding table 9, so as to drive the sample outlet needle frame to move back and forth along the second sliding table 9 (the sample outlet needle 3 moves along the X-axis direction).

The inlet ends of the 4 sample outlet needles 3 are respectively connected with the output ends of the 4 sample outlet pipes, the middle parts of the 4 sample outlet pipes are respectively clamped in the 4 channels of the second 4-channel peristaltic pump 27, and the input ends of the 4 sample outlet pipes penetrate through the sample outlet holes 1705 on the reaction cup sealing cover 17.

Optionally, two groove photoelectric sensors are mounted on the first sliding table 8, and a second up-down moving origin and a second up-down moving destination are set respectively; two groove photoelectric sensors are arranged on the second sliding table 9, and a second front-back movement origin and a second front-back movement terminal are respectively set for limiting the upper-lower and front-back limit movement positions of the movement of the sample needle 3. Setting the default starting position of the sample outlet needle when the right end of the sample outlet needle frame coincides with the second up-down moving origin and the second up-down moving origin, wherein the default starting position of the sample outlet needle is positioned right above the waste liquid tank 402; when the sample outlet needle is positioned at the default starting position, the inlet end of the sample outlet needle 3 is higher than the top end of the spiral cover centrifuge tube 5 by no less than 2cm. When the right end of the sample outlet needle frame coincides with the second up-and-down moving origin and the second back-and-forth moving end point, the four sample outlet needles 3 are positioned right above the first centrifuge tubes 502.

The collecting unit further comprises a third XY axis sliding table and 4 cap screwing motors 501, the cap screwing motors 501 are installed on the cap screwing motor frame in parallel at equal intervals, and the interval between every two adjacent cap screwing motors 501 is the central distance of the adjacent centrifuge tube holes of each row of centrifuge tube frames in the collecting disc 4; and a third XY-axis sliding table is fixedly arranged on the inner wall of the device box body 1, and one end of the spiral cover motor frame is fixedly connected to the third XY-axis sliding table.

Specifically, the third XY axis sliding table includes a fifth sliding table 12 and a sixth sliding table 13, the sixth sliding table 13 is fixedly mounted on the device box body 1 along the horizontal direction and located above the second sliding rail 401, the fifth sliding table 12 is fixedly mounted on a slider of the sixth sliding table 13 along the vertical direction, and the left end of the cap screwing motor frame is fixedly connected to the slider of the fifth sliding table 12. The third XY axis sliding table is used for driving the cap screwing motor 501 to move back and forth and up and down respectively: under the drive of the fifth sliding table 12 motor, the rotary motion of the screw of the fifth sliding table 12 is converted into the upward or downward linear motion of the sliding block of the fifth sliding table 12, so as to drive the cap screwing motor frame to move up and down along the fifth sliding table 12 (the cap screwing motor 501 moves along the Y-axis direction); similarly, under the driving of the sixth sliding table 13 motor, the rotary motion of the screw of the sixth sliding table 13 is converted into a forward or backward linear motion of the slider of the sixth sliding table 13, so as to drive the cap screwing motor frame to move back and forth along the sixth sliding table 13 (the cap screwing motor 501 moves along the X-axis direction).

Optionally, two groove photoelectric sensors are mounted on the fifth sliding table 12, and a third up-down moving origin and a third up-down moving destination are set respectively; two groove photoelectric sensors are mounted on the sixth sliding table 13, and a third forward-backward movement origin and a third forward-backward movement destination are respectively set for limiting the up-down and forward-backward limit movement positions of the cap screwing motor 501. When the right end of the cap screwing motor frame is superposed with a third up-and-down moving origin and a third up-and-down moving origin, a default starting position of the cap screwing motor is set, and the default starting position of the cap screwing motor is positioned in front of the collecting tray 4; when the centrifugal pump is located at the default starting position of the screw cap motor, the bottom end of the screw cap motor 501 is higher than the top end of the centrifugal tube 5 by not less than 2cm. When the right end of the cap screwing motor frame coincides with the third up-and-down moving origin and the third back-and-forth moving end point, the four cap screwing motors 501 are respectively located right above the screw caps of the four fourth batch centrifuge tubes 505.

Optionally, the first sliding table 8 motor, the second sliding table 9 motor, the fifth sliding table 12 motor, the sixth sliding table 13 motor, and the cap screwing motor 501 are all electrically connected to the control unit 30. According to the present embodiment, the spiral cover of the spiral cover centrifuge tube 5 is unscrewed counterclockwise and removed, the sample is taken out, the spiral cover motor 501 which picks up the spiral cover runs to the upper part of the spiral cover centrifuge tube 5 and screws the spiral cover of the spiral cover centrifuge tube 5 clockwise, and the operation processes of cleaning and disinfection and the control logic are performed.

The sampling working principle is as follows: when the reaction of the first batch of samples in the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 is finished and the first batch of reaction solution to be obtained needs to be sampled, the sixth sliding table 13 motor drives the screw cap motor 501 to move backwards by 10cm from the default starting position of the screw cap motor to reach the position right above the first batch of centrifuge tubes 502, then the fifth sliding table 12 motor drives the screw cap motor 501 to move downwards to the third up-down moving end point, at this time, the screw cap of the first batch of centrifuge tubes 502 is grasped by the grasping clamp of the screw cap motor 501, the screw cap of the first batch of centrifuge tubes 502 is unscrewed anticlockwise, and the screw cap of the first batch of centrifuge tubes 502 is picked up and then driven by the fifth sliding table 12 motor and the sixth sliding table 13 motor to return to the default starting position of the screw cap motor. The second slip table 9 motor drives out appearance needle 3 and moves 40cm (second back-and-forth movement terminal point promptly) forward from the default initial position of a kind needle, reachs directly over first batch centrifuging tube 502, then first slip table 8 motor drives out appearance needle 3 downstream to the second and reciprocates the terminal point, and the appearance needle 3 exit end of giving out a sample this moment stretches into first batch centrifuging tube 502 and goes out the appearance. Meanwhile, the inlet ends of the 4 sample outlet needles 3 are respectively connected with the output ends of the 4 sample outlet pipes, the input ends of the 4 sample outlet pipes respectively penetrate through the sample holes 1705 and extend into the bottoms of the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610, and under the action of the second 4-channel peristaltic pump 27, reaction liquid in the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 can be respectively collected into four first centrifuge tubes 502 through the sample outlet pipes by the 4 sample outlet needles 3, so that sample outlet of the first reaction liquid in the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 can be realized. After the operation of drawing a design, rethread sixth slip table 13 motor drives spiral cover motor 501 who has picked up the spiral cover and follows the acquiescent initial position of spiral cover motor rearward movement 10cm, arrive directly over first centrifuging tube 502, then fifth slip table 12 motor drives spiral cover motor 501 downstream to the third terminal point that reciprocates, this moment spiral cover motor 501's grab clamp is screwed up the spiral cover of first centrifuging tube 502 on first centrifuging tube 502 clockwise, last spiral cover motor 501 is again fifth slip table 12 motor, the acquiescent initial position of spiral cover motor is got back to under the drive of sixth slip table 13 motor.

Similarly, if the sixth sliding table 13 motor drives the screw cap motor 501 to move 20cm, 30cm and 40cm backward from the default starting position of the screw cap motor, and then the fifth sliding table 12 motor drives the screw cap motor 501 to move downward to the third up-down moving end point, the corresponding second batch of centrifuge tubes 503, the third batch of centrifuge tubes 504 and the fourth batch of centrifuge tubes 505 can be unscrewed respectively, and the screw caps of the second batch of centrifuge tubes 503, the third batch of centrifuge tubes 504 and the fourth batch of centrifuge tubes 505 are picked up respectively and then returned to the default starting position of the screw cap motor. The second sliding table 9 motor drives the sample discharging needle 3 to move forwards by 30cm, 20cm and 10cm from the default starting position of the sample discharging needle, then the first sliding table 8 motor drives the sample discharging needle 3 to move downwards to a second up-and-down moving end point, and the outlet end of the sample discharging needle 3 can be controlled to extend into a second batch of centrifuge tubes 503, a third batch of centrifuge tubes 504 and a fourth batch of centrifuge tubes 505 to discharge samples respectively; under the action of the second 4-channel peristaltic pump 27, the second batch of reaction liquid, the third batch of reaction liquid and the fourth batch of reaction liquid can be respectively collected into the second batch of centrifuge tube 503, the third batch of centrifuge tube 504 and the fourth batch of centrifuge tube 505 through the sample outlet needle 3 and the sample outlet pipeline, so that the sample outlet function of different batches of samples is realized. After the sample outlet operation is finished, the screw cap motor 501 which picks up the screw cap is driven by the motor of the sixth sliding table 13 to move backwards by 20cm, 30cm and 40cm from the default starting position of the screw cap motor, then the motor of the fifth sliding table 12 drives the screw cap motor 501 to move downwards to the third up-down moving end point, the screw caps of the

centrifuge tubes

503, 504 and 505 in the second batch, are screwed up counterclockwise respectively by the grippers of the screw cap motor 501, and finally, the screw cap motor returns to the default starting position of the screw cap motor under the driving of the motor of the fifth sliding table 12 and the motor of the sixth sliding table 13.

Optionally, the sample outlet needles 3 are driven by the first sliding table 8 motor and the second sliding table 9 motor to move up and down and back and forth respectively, the sample outlet needles 3 can return to default initial positions of the sample outlet needles, and waste liquid generated after cleaning and disinfecting the sample inlet needles, the sample inlet pipeline, the reaction cups 16 and the sample outlet pipeline is discharged to the waste liquid tank 402.

Optionally, the first sliding table 8 motor, the second sliding table 9 motor, the third sliding table 10 motor, the fourth sliding table 11 motor, the seventh sliding table 14 motor, the fifth sliding table 12 motor, the sixth sliding table 13 motor, the circular reaction turntable motor 1803, the cap screwing motor 501, the power switch of the pH meter 15, the power switch of the ultraviolet lamp 19, the magnetic stirrer 25, the first 4-channel peristaltic pump 26, the second 4-channel peristaltic pump 27, the quintuplet valve group 28 power supply, the first diaphragm pump 31 power supply, the second diaphragm pump 32 power supply, the first quantitative injection pump 33 power supply, the second quantitative injection pump 34 power supply, the third quantitative injection pump 35 power supply, and the liquid level sensor 36 power supply are all electrically connected to the control unit 30. According to the operation flows and the control logic of sample introduction, reaction, sample discharge, cleaning, disinfection and waste liquid discharge provided by the embodiment, industrial design technicians in the field can carry out industrial automatic control on the operation process of the device through a known industrial control program, so that the workload of the operators can be reduced, and the experimental efficiency is improved.

In an optional embodiment, when the motor of the third slide table 10 is controlled to drive the injection needle 2 to move upward, the control unit 30 sends a specific level signal to a port connected to the motor of the third slide table 10, and the motor of the third slide table 10 responds to the specific level signal after receiving the specific level signal, and starts to drive the threaded rod inside the third slide table 10 to rotate clockwise, so as to drive the injection needle 2 to move upward.

In the embodiment, a method for enriching viruses in sewage at high flux is also provided, which is realized by adopting the device for enriching viruses in sewage at high flux, and comprises the following steps:

s1, preparing a sample and a centrifuge tube: respectively loading a first batch of samples, a second batch of samples, a third batch of samples and a fourth batch of samples into a first batch of sampling bottles 702, a second batch of sampling bottles 703, a third batch of sampling bottles 704 and a fourth batch of sampling bottles 705, and placing the first batch of sampling bottles 702, the second batch of sampling bottles 703, the third batch of sampling bottles 704 and the fourth batch of sampling bottles 705 on 4 rows of sampling bottle racks of a sample tray 6; placing a first batch of centrifuge tubes 502, a second batch of centrifuge tubes 503, a third batch of centrifuge tubes 504 and a fourth batch of centrifuge tubes 505 on 4-row centrifuge tube racks of a collecting tray 4 respectively;

s2, disinfection and cleaning before reaction: the sample injection needle 2 is driven by the first XY axis sliding table to sequentially enter the water cleaning tank 601 and the disinfectant tank 602, and the cleaning and disinfection of the sample injection needle 2, the sample injection pipeline, the four reaction cups, the sample outlet pipeline and the sample outlet needle 3 are sequentially realized; the sample injection needle 2 is driven by the motors of the third sliding table 10 and the fourth sliding table 11 to enter the water cleaning tank 601, pure water is injected into the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 through the sample injection needle 2 and the sample injection pipeline under the action of the peristaltic pump 26 with the first 4 channels, so that the sample injection needle 2, the sample injection pipeline, the four reaction cups, the sample outlet pipeline and the sample outlet needle 3 are cleaned, and waste liquid formed after cleaning is discharged to the waste liquid tank 402 through the sample outlet pipeline and the sample outlet needle 3; then, the third sliding table 10 motor and the fourth sliding table 11 motor drive the sample injection needle 2 to enter the disinfectant tank 602, under the action of the first 4-channel peristaltic pump 26, a disinfectant is injected into the four reaction cups through the sample injection needle 2 and the sample injection pipeline, so that the sample injection needle 2, the sample injection pipeline, the four reaction cups, the sample outlet pipeline and the sample outlet needle 3 are disinfected, and waste liquid formed after disinfection is discharged to the waste liquid tank 402 through the sample outlet pipeline and the sample outlet needle 3;

the waste liquid in the waste liquid tank 402 enters the sewage treatment system through the waste liquid pipeline 29.

S3, sample injection reaction: drive through first XY axle slip table and advance kind needle 2 and stretch into first batch sampling bottle 702 bottom and sample, promptly through third slip table 10 motor, 11 motors in fourth slip table drive 4 and advance kind needle 2 and stretch into first batch sampling bottle 702 bottom and sample, the 14 motors in seventh slip table drive the 15 electrodes of pH meter and move down to the fourth terminal point that reciprocates, under the effect of first 4 passageway peristaltic pumps 26, through advancing kind needle 2 and advance kind pipeline to first reaction cup 1601, fourth reaction cup 1604, seventh reaction cup 1607, inject into first batch sample in the tenth reaction cup 1610.

Then, under the control of a quintuplet valve group 28, aluminum chloride is respectively injected into the four reaction cups through a third quantitative injection pump 35, and after stirring is carried out for 2min under the stirring condition provided by a magnetic stirrer 1802 and a magnetic stirrer 25, if the pH value of the sample measured by a pH meter 15 is within an acceptable pH value range of the sample, a flocculating constituent is generated through reaction; if the pH value of the sample measured by the pH meter 15 is not within the acceptable pH value range of the sample, the pH value of the sample is adjusted: under the control of the quintuplet valve group 28, hydrochloric acid and/or sodium hydroxide are injected into the four reaction cups through a first quantitative injection pump 33 and a second quantitative injection pump 34 until the pH value of the sample is within an acceptable pH value range of the sample, so that the pH value of the sample can be quickly adjusted, and a flocculating constituent is generated through reaction;

optionally, the acceptable pH range of the sample can be set according to the requirements of the actual application; in a preferred embodiment, the acceptable sample pH range is 6 ± 0.3, i.e. 5.7 to 6.3.

Optionally, the method for adjusting the pH of the sample in step S3 includes the following steps:

s31, judging the pH value adjusting mode of the sample: if the sample pH is within the coarse adjustment range (in a preferred embodiment, the coarse adjustment range is the sample pH < 3 or the sample pH > 9), then go to step S32; if the pH of the sample is within the middle adjustment range (in a preferred embodiment, the middle adjustment range is 3. Ltoreq. Sample pH < 5 or 7. Ltoreq. Sample pH < 9), then go to step S33; if the pH of the sample is within the fine tuning range (in a preferred embodiment, the fine tuning range is 5. Ltoreq. Sample pH < 5.7 or 6.3. Ltoreq. Sample pH < 7), go to step S34;

s32, coarse adjustment of pH value: adding a set coarse adjustment volume of sodium hydroxide if the sample pH is in the low coarse adjustment interval within the coarse adjustment interval (in a preferred embodiment, the low coarse adjustment interval within the coarse adjustment interval is a sample pH < 3, and if the sample pH < 3, then 0.8ml of sodium hydroxide is added); adding a set coarse volume of hydrochloric acid if the sample pH is in a high coarse adjustment interval within the coarse adjustment interval (in a preferred embodiment, the high coarse adjustment interval within the coarse adjustment interval is a sample pH > 9, and if the sample pH > 9, then adding 0.7ml of hydrochloric acid); then stirring for 30s, reading the stable pH value of the sample after coarse adjustment by the pH meter 15, judging whether the pH value of the sample after coarse adjustment is within an acceptable pH value range of the sample (in a preferred embodiment, judging whether the pH value of the sample after coarse adjustment is within a range of 6 +/-0.3), if the pH value of the sample after coarse adjustment is within the acceptable pH value range of the sample, reacting to generate a flocculating constituent, otherwise, returning to the step S31;

s33, adjusting the pH value: adding a set medium volume of sodium hydroxide if the pH of the sample is in a low-medium range within the medium range (in a preferred embodiment, the pH of the sample is 3. Ltoreq. In the low-medium range within the medium range and less than 5, and adding 0.15ml of sodium hydroxide if the pH of the sample is 3. Ltoreq. In the sample pH less than 5); if the pH value of the sample is in a high-medium regulation interval in a medium regulation interval, adding hydrochloric acid with a set medium regulation volume (in a preferred embodiment, the high-medium regulation interval in the medium regulation interval is 7 < the pH value of the sample is less than or equal to 9, and if the pH value of the sample is 7 < the pH value of the sample is less than or equal to 9, adding 0.12ml of hydrochloric acid); then, stirring 30s, reading the stable pH value of the sample after the intermediate adjustment by the pH meter 15, judging whether the pH value of the sample after the intermediate adjustment is within an acceptable pH value range of the sample (in a preferred embodiment, judging whether the pH value of the sample after the intermediate adjustment is within a range of 6 +/-0.3), if the pH value of the sample after the intermediate adjustment is within the acceptable pH value range of the sample, reacting to generate a flocculating constituent, otherwise, returning to the step S31;

s34, fine adjustment of pH value: if the pH value of the sample is in a low fine adjustment interval in the fine adjustment interval, adding sodium hydroxide with a set fine adjustment volume (in a preferred embodiment, the pH value of the sample is less than or equal to 5 and less than 5.7 in the low fine adjustment interval in the fine adjustment interval, and if the pH value of the sample is less than or equal to 5 and less than 5.7, adding 0.05ml of sodium hydroxide); adding a set trim volume of hydrochloric acid if the pH of the sample is in a high trim interval within the trim interval (in a preferred embodiment, the high trim interval within the trim interval is 6.3 < pH of the sample.ltoreq.7, and if 6.3 < pH of the sample.ltoreq.7, then adding 0.05ml of hydrochloric acid); then stirring for 30S, reading the stable pH value of the sample after fine adjustment by the pH meter 15, judging whether the pH value of the sample after fine adjustment is within an acceptable pH value range of the sample (in a preferred embodiment, judging whether the pH value of the sample after middle adjustment is within a range of 6 +/-0.3), if the pH value of the sample after fine adjustment is within the acceptable pH value range of the sample, reacting to generate a flocculating constituent, otherwise, returning to the step S31;

in the process of roughly adjusting the pH value and the middle adjusting the pH value, the volumes of the added hydrochloric acid and the added sodium hydroxide are set to be unequal, so that the situation that the pH value of the sample possibly rebounds back and forth after acid and alkali neutralization in the process of adjusting the pH value of the sample is avoided.

S4, transfer and collection: when the reaction of the first batch of samples in the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 is finished and the obtained first batch of reaction liquid needs to be sampled, the sixth sliding table 13 motor drives the screw cap motor 501 to move backwards by 10cm from the default starting position of the screw cap motor to reach the position right above the first batch of centrifuge tubes 502, then the fifth sliding table 12 motor drives the screw cap motor 501 to move downwards to the third up-and-down moving end point, at this time, the screw cap of the first batch of centrifuge tubes 502 is grasped by the grasping clamp of the screw cap motor 501, the screw cap of the first batch of centrifuge tubes 502 is unscrewed anticlockwise, the screw cap of the first batch of centrifuge tubes 502 is picked up and then is driven by the fifth sliding table 12 motor and the sixth sliding table 13 motor to return to the default starting position of the screw cap motor, and the interference of the subsequent sample discharge needles 3 is avoided;

the motor of the second sliding table 9 drives the sample outlet needle 3 to move forward 40cm (namely, a second front-and-back movement end point) from the default starting position of the sample outlet needle to reach the position right above the first batch of centrifuge tubes 502, then the motor of the first sliding table 8 drives the sample outlet needle 3 to move downward to the second front-and-back movement end point, and at the moment, the outlet end of the sample outlet needle 3 extends into the first batch of centrifuge tubes 502 to carry out sample outlet; meanwhile, the inlet ends of the 4 sample outlet needles 3 are respectively connected with the output ends of the 4 sample outlet pipes, the input ends of the 4 sample outlet pipes respectively penetrate through the sample holes 1705 and extend into the bottoms of the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610, and under the action of the second 4-channel peristaltic pump 27, the reaction liquid in the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 can be respectively transferred and collected into four first centrifuge tubes 502 through the sample outlet pipes by the 4 sample outlet needles 3, so that the sample outlet of the first reaction liquid in the first reaction cup 1601, the fourth reaction cup 1604, the seventh reaction cup 1607 and the tenth reaction cup 1610 can be realized; after the reaction liquid is transferred, the sample outlet needle 3 returns to the default initial position of the sample outlet needle under the driving of the first sliding table 8 motor and the second sliding table 9 motor;

after the operation of appearance of going out, rethread sixth slip table 13 motor drives spiral cover motor 501 who has picked up the spiral cover and follows the acquiescence initial position of spiral cover motor rearward movement 10cm, directly over the first centrifuging tube 502, then fifth slip table 12 motor drives spiral cover motor 501 downstream to the third and reciprocates the terminal point, this moment spiral cover motor 501's grab clamp is clockwise screwed up the spiral cover of first centrifuging tube 502 on first centrifuging tube 502, last spiral cover motor 501 is again at fifth slip table 12 motor, get back to the acquiescence initial position of spiral cover motor under the drive of sixth slip table 13 motor.

S5, disinfection and cleaning after reaction: after the reaction of the first batch of samples is finished and the first batch of reaction liquid is sampled, the third sliding table 10 motor and the fourth sliding table 11 motor drive the sample injection needle 2 to enter the water cleaning tank 601, under the action of the first 4-channel peristaltic pump 26, pure water is injected into the four reaction cups through the sample injection needle 2 and the sample injection pipeline, so that the cleaning of the sample injection needle 2, the sample injection pipeline, the four reaction cups, the sample outlet pipeline and the sample outlet needle 3 is realized, and waste liquid formed after cleaning is discharged to the waste liquid tank 402 through the sample outlet pipeline and the sample outlet needle 3; then, the third sliding table 10 motor and the fourth sliding table 11 motor drive the sample injection needle 2 to enter the disinfectant cleaning tank 602, under the action of the first 4-channel peristaltic pump 26, a disinfectant is injected into the four reaction cups through the sample injection needle 2 and the sample injection pipeline, so that the sample injection needle 2, the sample injection pipeline, the four reaction cups, the sample outlet pipeline and the sample outlet needle 3 are disinfected, and waste liquid formed after disinfection is discharged to the waste liquid tank 402 through the sample outlet pipeline and the sample outlet needle 3;

optionally, a seventh sliding table 14 motor drives the electrode of the pH meter 15 to move upward to a fourth up-and-down moving origin, a reaction turntable motor 1803 drives the circular reaction turntable 18 to rotate counterclockwise by 30 °, at this time, four reaction cup sealing covers 17 are respectively located right above the second reaction cup 1602, the fifth reaction cup 1605, the eighth reaction cup 1608, and the eleventh reaction cup 1611, the seventh sliding table 14 motor drives the electrode of the pH meter 15 to move downward to a fourth up-and-down moving destination, the third sliding table 10 motor and the fourth sliding table 11 motor drive the sample injection needle 2 to enter the water cleaning tank 601, under the action of the first 4-channel peristaltic pump 26, pure water is injected into the four cleaning cups through the sample injection needle 2 and the sample injection pipeline, so as to realize secondary cleaning of the sample injection needle 2, the sample injection pipeline, the sample outlet pipeline, and the sample outlet needle 3, and waste liquid formed after cleaning is discharged to the waste liquid tank 402 through the sample outlet pipeline and the sample outlet needle 3.

S6, switching reaction cups, repeating the steps S2 to S5, and sequentially processing samples of different batches: the electrode of the pH meter 15 is driven by the seventh sliding table 14 motor to move upwards to a fourth up-down moving original point, the reaction turntable motor 1803 drives the circular reaction turntable 18 to rotate anticlockwise by 30 degrees, and at the moment, the four reaction cup sealing covers 17 are respectively positioned right above the third reaction cup 1603, the sixth reaction cup 1606, the ninth reaction cup 1609 and the twelfth reaction cup 1612;

and (3) repeating the steps S2 to S5 according to the number of the samples, and sequentially processing different batches of samples: according to the batch of sampling bottles to be processed, the control unit 30 controls the first XY axis sliding table, the second XY axis sliding table and the third XY axis sliding table to repeat the processes of disinfection and cleaning before reaction, sample introduction reaction, transfer collection and disinfection and cleaning after reaction in the steps S2-S5, so that the second batch of samples in the second batch of sampling bottles 703, the third batch of samples in the third batch of sampling bottles 704 and the fourth batch of samples in the fourth batch of sampling bottles 705 can be sequentially processed, the reaction of different batches of samples is realized, and the time is greatly shortened.

Optionally, the first sliding table 8 motor, the second sliding table 9 motor, the third sliding table 10 motor, the fourth sliding table 11 motor, the seventh sliding table 14 motor, the reaction rotary table motor 1803, the first 4-channel peristaltic pump 26, the second 4-channel peristaltic pump 27, and the quintuplet valve group 28 are controlled by the control unit 30, so that the processes of sample preparation, disinfection and cleaning before reaction, sample feeding reaction, transfer collection, disinfection and cleaning after reaction, automatic reaction cup switching and the like in the above steps S1 to S6 are realized by the control unit 30.

The apparatus of the present invention is not limited to the embodiments described in the detailed description, which are merely illustrative of the present invention, but the present invention may be embodied in other specific forms or other specific forms without departing from the spirit or essential characteristics thereof. Any variations that are equivalent to the intent and scope of the claims are also intended to be included within the scope of the invention.

Claims

1. A device for high-flux enrichment of viruses in sewage is characterized by comprising: the device comprises a device box body (1), a control unit (30), a reagent unit, a sample unit, a reaction unit and a collection unit, wherein the control unit (30), the reagent unit, the sample unit, the reaction unit and the collection unit are arranged in the device box body (1); the bottom of the device box body (1) is provided with a vertical clapboard, and the reagent unit and the sample unit are respectively arranged at two sides of the vertical clapboard; the middle part of the device box body (1) is provided with a horizontal clapboard;

the reagent unit comprises a pure water tank (23), a disinfectant tank (24), an acid liquor tank (20), an alkali liquor tank (21) and a flocculant tank (22);

the device comprises a sample unit, a sample unit and a sample unit, wherein the sample unit comprises a sample disc (6), a water cleaning tank (601), a disinfectant tank (602), a first slide rail (603), a sampling bottle (7), a sampling bottle rubber cover (701), a first XY axis sliding table and 4 sampling needles (2), the first slide rail (603) is fixedly installed on a device box body (1), the device box body (1) is in sliding connection with the sample disc (6) through the first slide rail (603), the sample disc (6) comprises a plurality of rows of sampling bottle racks, and 4 sampling bottle holes with specified intervals are arranged on each row of sampling bottle racks and used for placing the sampling bottles (7); the sample injection needles (2) are arranged on the sample injection needle frame side by side at the specified interval, the first XY-axis sliding table is fixedly arranged on the vertical partition plate, and one end of the sample injection needle frame is fixedly connected to the first XY-axis sliding table; the pure water tank (23) is connected with the water cleaning tank (601); the disinfectant tank (24) is connected with the disinfectant tank (602);

the reaction unit comprises 12 reaction cups (16), a reaction cup sealing cover (17), a circular reaction turntable (18), a pH meter support (1501), a pH meter (15) and a seventh sliding table (14), the support of the circular reaction turntable (18) is fixedly arranged on the horizontal partition plate, the circular reaction turntable (18) comprises 12 reaction cup holes with the same size, and the circle centers of the 12 reaction cup holes are uniformly distributed on a concentric circle of the circumference of the circular reaction turntable (18) at equal intervals; the seventh sliding table (14) is fixedly arranged on a back plate of the device box body (1), and the pH meter bracket (1501) is a cross-shaped bracket and is used for supporting and fixing an electrode of the pH meter (15); 4 end parts of the cross-shaped bracket are respectively fixedly connected with the top parts of 4 reaction cup sealing covers (17), and one end of the pH meter bracket (1501) is fixedly connected to a seventh sliding table (14) sliding block; the acid solution tank (20) is connected with acid solution holes (1701) on four reaction cup sealing covers (17) through pipelines; the alkaline solution tank (21) is connected with alkaline solution holes (1702) on four reaction cup sealing covers (17) through pipelines; the flocculant tank (22) is connected with flocculant holes (1703) on four reaction cup sealing covers (17) through pipelines; each sample injection needle (2) is respectively connected with a sample injection hole (1704) on a reaction cup sealing cover (17) through a sample injection pipeline;

the collecting unit comprises a collecting disc (4), a waste liquid groove (402), spiral cover centrifuge tubes (5), a spiral cover motor (501), a second XY-axis sliding table, a third XY-axis sliding table and 4 sample discharging needles (3), a second sliding rail (401) is fixedly mounted on the device box body (1), the device box body (1) is connected with the collecting disc (4) in a sliding mode through the second sliding rail (401), the collecting disc (4) comprises a plurality of rows of centrifuge tube frames, and 4 centrifuge tube holes with preset intervals are formed in each row of centrifuge tube frames and used for containing the spiral cover centrifuge tubes (5); the sample discharging needles (3) are arranged on the sample discharging needle frame side by side at the preset interval, and the cover rotating motors (501) are arranged on the cover rotating motor frame side by side at the preset interval; a second XY-axis sliding table is fixedly arranged on the upper part of the vertical partition plate, and one end of the sample outlet needle frame is fixedly connected to the second XY-axis sliding table; a third XY-axis sliding table is fixedly arranged on the device box body (1), and one end of the spiral cover motor frame is fixedly connected to the third XY-axis sliding table; each sample outlet needle (3) is respectively connected with a sample outlet hole (1705) on a reaction cup sealing cover (17) through a sample outlet pipeline.

2. The device for high-flux enrichment of viruses in sewage according to claim 1, wherein: the reagent unit is provided with a first diaphragm pump (31), a second diaphragm pump (32), a first quantitative injection pump (33), a second quantitative injection pump (34), a third quantitative injection pump (35) and a quintuplet valve group (28);

the pure water tank (23) is connected with the inlet end of the first diaphragm pump (31) through a hose, and the output end of the first diaphragm pump (31) is connected with the water cleaning tank (601) through a hose; the disinfectant tank (24) is connected with the inlet end of the second diaphragm pump (32) through a hose, and the output end of the second diaphragm pump (32) is connected with the disinfectant tank (602) through a hose;

the acid solution tank (20) is connected with a first quantitative injection pump (33) through a pipeline controlled by an input valve of a first quintuplet valve group, and four outlet pipelines respectively controlled by four output valves of the first quintuplet valve group respectively penetrate through acid solution holes (1701) on four reaction cup sealing covers (17); the alkaline solution tank (21) is connected with a second quantitative injection pump (34) through a pipeline controlled by an input valve of a second quintuplet valve group, and four outlet pipelines respectively controlled by four output valves of the second quintuplet valve group respectively penetrate alkaline solution holes (1702) on four reaction cup sealing covers (17); the flocculant tank (22) is connected with a third quantitative injection pump (35) through a pipeline controlled by an input valve of a third quintuplet valve group, and four outlet pipelines respectively controlled by four output valves of the third quintuplet valve group respectively penetrate flocculant holes (1703) on four reaction cup sealing covers (17);

the acid liquor tank (20) is used for storing hydrochloric acid; the alkali liquor tank (21) is used for storing sodium hydroxide; the flocculant tank (22) is used for storing flocculant, and the flocculant is aluminum chloride;

the cap screwing motor (501), a power switch of the pH meter (15), a power supply of the quintuplet valve group (28), a power supply of the first diaphragm pump (31), a power supply of the second diaphragm pump (32), a power supply of the first quantitative injection pump (33), a power supply of the second quantitative injection pump (34) and a power supply of the third quantitative injection pump (35) are respectively and electrically connected with the control unit (30).

3. The device for high-throughput enrichment of viruses in sewage according to claim 1, wherein: liquid level sensors (36) are respectively arranged in the pure water tank (23), the disinfectant tank (24), the acid liquor tank (20), the alkali liquor tank (21) and the flocculating agent tank (22);

the power supply of the liquid level sensor (36) is electrically connected with the control unit (30).

4. The device for high-flux enrichment of viruses in sewage according to claim 1, wherein: the specified distance is the central distance between adjacent sampling bottle holes in each row of sampling bottle racks;

the outlet end of each sample injection needle (2) is respectively connected with the input end of a sample injection pipeline, each sample injection pipeline is respectively clamped in one channel of the first 4-channel peristaltic pump (26), and the output end of each sample injection pipeline penetrates through a sample injection hole (1704) on a reaction cup sealing cover (17);

the preset distance is the central distance of adjacent centrifuge tube holes in each row of centrifuge tube frames;

the inlet end of each sample outlet needle (3) is respectively connected with the output end of a sample outlet pipeline, each sample outlet pipeline is respectively clamped in one channel of the second 4-channel peristaltic pump (27), and the input end of each sample outlet pipeline respectively penetrates through a sample outlet hole (1705) on a reaction cup sealing cover (17);

the first 4-channel peristaltic pump (26) and the second 4-channel peristaltic pump (27) are respectively and electrically connected with the control unit (30).

5. The device for high-flux enrichment of viruses in sewage according to claim 1, wherein: the reaction unit is positioned above the horizontal partition plate, the reagent unit is positioned below the horizontal partition plate, and the collection unit is positioned above the sample unit;

the sample tray (6) comprises 4 rows of sampling bottle racks, and the collection tray (4) comprises 4 rows of centrifuge tube racks;

ultraviolet lamps (19) are arranged above the sample disc (6), above the collection disc (4) and on the circular reaction rotating disc (18);

the control unit (30) is electrically connected with the power switch of the ultraviolet lamp (19). .

6. The device for high-flux enrichment of viruses in sewage according to claim 1, wherein: the first XY axis sliding table comprises a third sliding table (10) and a fourth sliding table (11), the fourth sliding table (11) is fixedly arranged on the vertical partition plate, the third sliding table (10) is fixedly arranged on a sliding block of the fourth sliding table (11), and the right end of the sample injection needle frame is fixedly connected to the sliding block of the third sliding table (10);

the third sliding table (10) and the fourth sliding table (11) are respectively provided with a groove photoelectric sensor;

the second XY axis sliding table comprises a first sliding table (8) and a second sliding table (9), the second sliding table (9) is fixedly arranged on the upper part of the vertical partition plate, the first sliding table (8) is fixedly arranged on a sliding block of the second sliding table (9), and the right end of the sample outlet needle frame is fixedly connected to the sliding block of the first sliding table (8);

the first sliding table (8) and the second sliding table (9) are provided with groove photoelectric sensors;

the third XY shaft sliding table comprises a fifth sliding table (12) and a sixth sliding table (13), the sixth sliding table (13) is fixedly installed on the device box body (1), the fifth sliding table (12) is fixedly installed on a sliding block of the sixth sliding table (13), and the left end of the cap screwing motor frame is fixedly connected to the sliding block of the fifth sliding table (12);

the fifth sliding table (12) and the sixth sliding table (13) are respectively provided with a groove photoelectric sensor;

a groove photoelectric sensor is arranged on the seventh sliding table (14);

the control unit (30) is electrically connected with a first sliding table (8) motor, a second sliding table (9) motor, a third sliding table (10) motor, a fourth sliding table (11) motor, a seventh sliding table (14) motor, a fifth sliding table (12) motor and a sixth sliding table (13) motor respectively.

7. The device for high-flux enrichment of viruses in sewage according to claim 1, wherein: the reaction unit also comprises a reaction turntable motor (1803), and the reaction turntable motor (1803) is arranged below the circular reaction turntable (18);

the electrode of the pH meter (15) sequentially penetrates through pH meter holes on the pH meter bracket (1501) and the reaction cup sealing cover (17);

the reaction cups (16) comprise a first reaction cup (1601), a second reaction cup (1602), a third reaction cup (1603), a fourth reaction cup (1604), a fifth reaction cup (1605), a sixth reaction cup (1606), a seventh reaction cup (1607), an eighth reaction cup (1608), a ninth reaction cup (1609), a tenth reaction cup (1610), an eleventh reaction cup (1611) and a twelfth reaction cup (1612), and are sequentially placed in 12 reaction cup holes clockwise;

the control unit (30) is electrically connected with the circular reaction turntable motor (1803).

8. The device for high-throughput enrichment of viruses in sewage according to claim 1, wherein: the reaction unit also comprises a magnetic stirrer (1802) and a magnetic stirrer (25), wherein the magnetic stirrer (1802) is placed inside the reaction cup (16); the magnetic stirrer (25) is arranged on the bracket of the round reaction turntable (18) and is positioned right below the reaction cup (16);

the magnetic stirrer (25) is electrically connected with the control unit (30);

the bottom of the circular reaction turntable (18) is provided with a universal wheel (1801).

9. A method for high-flux enrichment of viruses in sewage, which is realized based on the device for high-flux enrichment of viruses in sewage of any one of claims 1 to 8, and is characterized by comprising the following steps:

s1, preparing a sample and a screw cap centrifuge tube: respectively loading samples into a first batch of sampling bottles (702), a second batch of sampling bottles (703), a third batch of sampling bottles (704) and a fourth batch of sampling bottles (705), and placing the first batch of sampling bottles (702), the second batch of sampling bottles (703), the third batch of sampling bottles (704) and the fourth batch of sampling bottles (705) on 4 rows of sampling bottle racks of a sample tray (6);

respectively placing a first batch of centrifuge tubes (502), a second batch of centrifuge tubes (503), a third batch of centrifuge tubes (504) and a fourth batch of centrifuge tubes (505) on 4 rows of centrifuge tube racks of a collecting tray (4);

s2, disinfecting and cleaning: the sample injection needle (2) is driven to enter the water cleaning tank (601) through the first XY axis sliding table, and the sample injection needle (2), the sample injection pipeline, the first reaction cup (1601), the fourth reaction cup (1604), the seventh reaction cup (1607), the tenth reaction cup (1610), the sample outlet pipeline and the sample outlet needle (3) are cleaned; then, the sample injection needle (2) is driven by the first XY axis sliding table to enter the disinfectant tank (602) to disinfect the sample injection needle (2), the sample injection pipeline, the first reaction cup (1601), the fourth reaction cup (1604), the seventh reaction cup (1607), the tenth reaction cup (1610), the sample outlet pipeline and the sample outlet needle (3), and waste liquid after cleaning and disinfection is discharged to the waste liquid tank (402) through the sample outlet pipeline and the sample outlet needle (3);

s3, sample introduction reaction: driving a sample injection needle (2) to extend into the bottom of a first batch of sampling bottles (702) through a first XY axis sliding table for sampling, driving a pH meter (15) electrode to move downwards to a fourth up-and-down moving terminal point through a seventh sliding table (14) motor, and injecting a first batch of samples into a first reaction cup (1601), a fourth reaction cup (1604), a seventh reaction cup (1607) and a tenth reaction cup (1610) through the sample injection needle (2) and a sample injection pipeline;

respectively injecting aluminum chloride into the first reaction cup (1601), the fourth reaction cup (1604), the seventh reaction cup (1607) and the tenth reaction cup (1610), stirring for 2min, and reacting to generate floccules if the pH value of the sample measured by the pH meter (15) is within an acceptable pH value range of the sample; if the pH value of the sample measured by the pH meter (15) is not within the acceptable pH value range of the sample, adjusting the pH value of the sample until the pH value of the sample is within the acceptable pH value range of the sample, and reacting to generate a flocculating constituent;

s4, transfer and collection: when the reaction of the first batch of samples in the first reaction cup (1601), the fourth reaction cup (1604), the seventh reaction cup (1607) and the tenth reaction cup (1610) is finished, the third XY-axis sliding table drives the screw cap motor (501) to unscrew the screw caps of the first batch of centrifuge tubes (502) and picks up the screw caps of the first batch of centrifuge tubes (502) to return to the default initial positions of the screw cap motor;

the outlet end of the sample outlet needle (3) extends into the first batch of centrifuge tubes (502) to carry out sample outlet under the drive of the second XY axis sliding table; reaction liquid in the first reaction cup (1601), the fourth reaction cup (1604), the seventh reaction cup (1607) and the tenth reaction cup (1610) is respectively transferred and collected into four first centrifuge tubes (502) through sample outlet pipelines by 4 sample outlet needles (3), so that the sample outlet of the first reaction liquid in the first reaction cup (1601), the fourth reaction cup (1604), the seventh reaction cup (1607) and the tenth reaction cup (1610) is realized; after the reaction liquid is transferred, the sample outlet needle (3) returns to the default starting position of the sample outlet needle under the drive of the second XY axis sliding table;

then, a cap screwing motor (501) is driven by a third XY axis sliding table to screw the screw caps of the first batch of centrifuge tubes (502) on the first batch of centrifuge tubes (502), and the cap screwing motor (501) is driven by the third XY axis sliding table to return to the default starting position of the cap screwing motor;

s5, disinfection and cleaning after reaction: repeating the disinfection and cleaning process of the step S2; then a seventh sliding table (14) motor drives electrodes of a pH meter (15) to move upwards to a third up-down moving original point, a circular reaction turntable (18) rotates anticlockwise for 30 degrees, at the moment, four reaction cup sealing covers (17) are respectively positioned right above a second reaction cup (1602), a fifth reaction cup (1605), an eighth reaction cup (1608) and an eleventh reaction cup (1611), the seventh sliding table (14) motor drives the electrodes of the pH meter (15) to move downwards to a third up-down moving end point, a first XY axis sliding table drives a sample injection needle (2) to enter a water cleaning tank (601), pure water is injected into the second reaction cup (1602), the fifth reaction cup (1605), the eighth reaction cup (1608) and the eleventh reaction cup (1611) through a sample injection needle (2) and a sample injection pipeline, a sample outlet pipeline and a sample outlet needle (3) for secondary cleaning, and the cleaned waste liquid is discharged to a waste liquid tank (402) through the sample outlet pipeline and the sample outlet needle (3);

s6, switching reaction cups, repeating the steps S2 to S5, and sequentially processing samples of different batches: the electrode of the pH meter (15) is driven to move upwards to a third up-and-down moving origin through a seventh sliding table (14) motor, the circular reaction turntable (18) rotates anticlockwise by 30 degrees, and at the moment, the four reaction cup sealing covers (17) are respectively positioned right above a third reaction cup (1603), a sixth reaction cup (1606), a ninth reaction cup (1609) and a twelfth reaction cup (1612); and controlling the first XY axis sliding table, the second XY axis sliding table and the third XY axis sliding table by the control unit (30) to repeat the processes of disinfection and cleaning, sample injection reaction, transfer collection and disinfection and cleaning after reaction in the steps S2-S5, and sequentially processing a second batch of samples in a second batch of sampling bottles (703), a third batch of samples in a third batch of sampling bottles (704) and a fourth batch of samples in a fourth batch of sampling bottles (705).

10. The method for high-throughput enrichment of viruses in wastewater according to claim 9, wherein: the method for adjusting the pH value of the sample in the step S3 comprises the following steps:

s32, coarse adjustment of pH value: if the pH value of the sample is in a low coarse adjustment interval in the coarse adjustment interval, adding sodium hydroxide with a set coarse adjustment volume; if the pH value of the sample is in a high coarse adjustment interval in a coarse adjustment interval, adding hydrochloric acid with a set coarse adjustment volume; then stirring 30s, reading the stable pH value of the sample after rough adjustment by a pH meter 15, if the pH value of the sample after rough adjustment is within an acceptable pH value range of the sample, reacting to generate a flocculating constituent, otherwise, returning to the step S31;

s34, fine adjustment of pH value: if the pH value of the sample is in a low fine adjustment interval in the fine adjustment interval, adding sodium hydroxide with a set fine adjustment volume; if the pH value of the sample is in a high fine adjustment interval in the fine adjustment interval, adding hydrochloric acid with a set fine adjustment volume; and then stirring for 30s, reading the stable pH value of the sample after fine adjustment by the pH meter 15, reacting to generate a flocculating constituent if the pH value of the sample after fine adjustment is within an acceptable pH value range of the sample, and otherwise, returning to the step S31.