WO2021256821A1 - Procédé de culture de masse de cladocères à l'aide de cryptophytes - Google Patents

Procédé de culture de masse de cladocères à l'aide de cryptophytes Download PDF

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WO2021256821A1
WO2021256821A1 PCT/KR2021/007454 KR2021007454W WO2021256821A1 WO 2021256821 A1 WO2021256821 A1 WO 2021256821A1 KR 2021007454 W KR2021007454 W KR 2021007454W WO 2021256821 A1 WO2021256821 A1 WO 2021256821A1
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acid
daphnia
culture
water
flagellum
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PCT/KR2021/007454
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Korean (ko)
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강석중
정우철
최병대
김봉
엠디아니수자만
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경상국립대학교산학협력단
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/50—Culture of aquatic animals of shellfish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Definitions

  • the present invention relates to a method for mass culturing daphnia, and more particularly, to a method for mass culturing daphnia using silver flagellum.
  • Daphnia is a filter-feeding organism, and it is a species that consumes a wide range of food including yeast, ciliates and bacteria, as well as phytoplankton, a fistula (Urabe et al., Hydrobiologia 25: 121-128, 1991; Fileto et al., Aquat. Ecol). . 38: 503-514, 2004; Kumar et al, International Review of Hydrobiology 93:. 284-296, 2008).
  • Daphnia's growth rate and reproductive rate are affected by the breeding environment, such as natural or artificial culture, and the composition of the supplied food (Fileto et al. , Aquat Ecol 38: 503-514, 2004).
  • the composition of the supplied food Ferto et al. , Aquat Ecol 38: 503-514, 2004.
  • KCTC 1231BP silver flagellate teleauracus empioxia
  • a plant food organism for aquaculture a method for culturing worms and bivalves using the same.
  • the present invention is to solve various problems, including the above problems, by applying a heterotrophic culture of an organic medium under matte to effectively produce concentrated silver flagellum containing a large amount of omega-3 polyunsaturated fatty acids.
  • An object of the present invention is to provide a method for mass culturing daphnia using silver flagellum that can be cultured at a high density.
  • these problems are exemplary, and the scope of the present invention is not limited thereto.
  • NaOH sodium hydroxide
  • an organic medium with culture water containing silver flagellum and adding sodium hydroxide (NaOH) to prepare an alkaline hydrolyzate; an organic acid and natural water in the alkaline hydrolyzate Neutralization step of adding to neutralize; Fermentation step of fermenting the neutralized alkaline hydrolyzate under matte; centrifuging the fermentation broth that has undergone the fermentation step to obtain a concentrated silver flagellum algae from the supernatant; and adding humus to the lower layer of the fermented broth to prepare humic acid, a method for producing a feed composition for culturing crustaceans is provided.
  • NaOH sodium hydroxide
  • a feed composition for culturing crustaceans prepared by the above method.
  • 1 is a schematic diagram schematically showing the entire process of the method for mass culturing daphnia using silver flagellum of the present invention.
  • FIG. 2 is a photograph showing a test apparatus for protospecies separation of silver flagella and daphnia.
  • 3 is a graph comparing and analyzing the growth of silver flagellum progenitor and Chroomonas sp.
  • Figure 4 is a graph comparing and analyzing the growth of the progenitor species of silver flagella and Cryptomonas ovata.
  • 5 is a graph comparing and analyzing the growth of silver flagellum progenitor and Chilomonas paramecium.
  • FIG. 6 is a photograph showing a system for extracting humic acid using Humus.
  • A extraction part
  • B adjustment part for stabilization
  • C air stripping part
  • D storage tank.
  • FIG. 7 is a photograph showing the appearance of a 20-ton daphnia mass culture system.
  • FIG. 8 is a photograph showing the appearance of a product for low-temperature storage after collecting daphnia produced according to an embodiment of the present invention.
  • FIG. 9 is a conceptual diagram of a field application system connected to a seed culture tank among types of daphnia mass culture system of the present invention.
  • FIG. 10 is a photograph showing the appearance of an apparatus for producing aquatic seeds by linking a daphnia culture tank and a seed production system indoors.
  • A daphnia culture tank
  • B aquaculture seed tank (sea fish fry).
  • 11 is a graph comparing and analyzing the growth rate when daphnia cultured as concentrated silver flagellum of the present invention and commercial artemia, respectively, fed to black sea bream fry.
  • “cryptophytes” are unicellular protists living in freshwater and seawater, small in size (5-50 ⁇ m), easily disintegrated by environmental shocks, and mainly inhabiting cold water and somewhat deep waters. It refers to a group of organisms named 'crypto flagellum' because of their characteristic features. Most of them are photosynthetic organisms that produce unsaturated fatty acids, and are important industrial organisms such as aquaculture and omega-3 production.
  • “Cladocera” is a crustacean of the family Arthropoda, copepod, crustaceans, and Daphnia.
  • the body length of daphnia is 0.5-2.5 mm, and it is suitable as a living food for fish and crustaceans (shrimp, crab) fry. Accordingly, artificial culture is also made to use daphnia as feed for aquaculture fish species, but it is only a small-scale culture rather than mass production.
  • heterotroph means that bacteria, fungi, and parasitic plants cannot produce nutritious organic matter from inorganic matter, so it is necessary to take assimilation nutrients (organic matter) made by green plants directly or indirectly. It is called trophic or heterotrophic.
  • Crustacean as used in this document is a group of animals belonging to the crustaceans of the Arthropoda Crustaceae, Amphipodidae, and Daphnia, which inhabits both freshwater, brackish water and seawater, and refers to a planktonic type that floats on water. Distributed throughout the world, poor swimmers live in reservoirs and lakes, not flowing water. The small one is 0.2 mm and the large one is over 4 mm, and it is a multicellular organism with a heart, digestive system, and reproductive organs. It feeds on algae (phytoplankton), the bottom of the food chain, and feeds on small animals including young fish.
  • algae phytoplankton
  • NaOH sodium hydroxide
  • the Cryptomonad may be selected from the group comprising Chroomonas genus, Cryptmonas in, Rhodomonas in, Chilomonas genus and Cyanomonas in the neutralization step may be to adjust to pH 5 to 7, and the natural number may be fresh water, brackish water or sea water.
  • the fermentation step is adjusted to pH 6 to 8 and can be performed for 12 to 15 days at a temperature of 25 to 30 ° C.
  • the organic medium is livestock meal, farmhouse by-products, processed food by-products, sewage treatment plant sludge, food It may be selected from the group consisting of waste water, land farm solid waste and organic fertilizer, and the amount of the organic medium may be 0.1 to 5% by weight of the culture water.
  • 0.1 to 5% by weight of the hibiscus may be added, and the humus may be humus, peat moss or charcoal, and the crustaceans are Daphnia sp., Moina sp. It may be aquatic water flea ( Diaphanosoma sp. ) or salt lake water flea ( Moina mongolica ).
  • the organic acid may be acetic acid, citric acid, malic acid, benzoic acid, glacial acetic acid, lemon acid, sulfonic acid, tartaric acid or glycolic acid, and the concentrated silver flagellum algae may be supplied at 2,000 to 200,000 cell/mL.
  • an organic medium with culture water containing silver flagellum and adding sodium hydroxide (NaOH) to prepare an alkaline hydrolyzate; an organic acid and natural water in the alkaline hydrolyzate Neutralization step of adding to neutralize; Fermentation step of fermenting the neutralized alkaline hydrolyzate under matte; centrifuging the fermentation broth that has undergone the fermentation step to obtain a concentrated silver flagellum algae from the supernatant; and adding humus to the lower layer of the fermented broth to prepare humic acid, a method for producing a feed composition for culturing crustaceans is provided.
  • NaOH sodium hydroxide
  • the organic acid may be acetic acid, citric acid, malic acid, benzoic acid, glacial acetic acid, lemon acid, sulfonic acid, tartaric acid or glycolic acid.
  • a feed composition for culturing crustaceans prepared by the above method.
  • the crustaceans may be Daphnia sp., Moina sp., Radix water flea ( Diaphanosoma sp.), or saltho water flea ( Moina mongolica ).
  • the mass-cultured daphnia of the present invention can be used as feed for breeding fish or crustacean self-fining fish, and the fish are sea bream, red snapper, stone sea bream, gangdam sea bream, black sea bream, red sea bream, dot sea bream, beng edam, rockfish, yellow snapper, red sea bream, Kingfisherfish, Dageumbari, Daewangbari, White-spotted poisonous fishtail, Songmi, Rat songmi, Yangyang, 3rd century, Sugimi, Blackcurrant, Red Redfish, Saengbok, Geombok, Magpiebok, Hwangbok, Purple suit, Secret suit, Horsetail, Horsetail, Yellowtail, Conger eel, perch, perch, perch, perch, flounder, flounder, mullet, flounder, flounder, flounder, flounder, flounder, flounder, flounder, flounder, flounder, flounder, flounder, flounder, flounder, flounder
  • an advanced high-density culture has been proposed to solve various problems in culture due to low density and economical problems with the high-density batch culture method, which is a conventional artificial culture technique (Japanese Patent No. 3394028, 2003.01.31. ; Korean Utility Model No. 232078, 2001.05.15.)
  • the present inventors have conducted a number of studies for efficient mass production of daphnia, and as a result of many studies, pure silver flagellum is produced in large quantities by a cross-trophic culture method that matches the concentration and temperature conditions of organic substances, which are fermentation conditions, under no light without inoculation of the original species. and developed a culture method that can economically obtain highly concentrated silver flagellum by concentrating the supernatant. Therefore, it is possible to mass-produce daphnia, a food organism for fish and crustaceans, using silver flagellum, which is a unicellular protist living in freshwater and seawater, so it can be used industrially.
  • Korea Patent Publication No. 2002-0061066 discloses a high-density culturing method of Daphnia using a flow method
  • Korea Patent No. 0020354 discloses a fermented livestock manure fermented product after adding a useful microorganism mixture to livestock manure.
  • a feed composition for culturing daphnia as an active ingredient is disclosed, it is different from the method of mass culturing daphnia using the concentrated silver flagellum containing a large amount of omega-3 polyunsaturated fatty acids of the present invention.
  • the present invention has been derived from research and development through trial and error over decades in response to the urgent needs of the aquaculture seed industry, and is intended to solve the following problems:
  • First production of seeds of seawater fish and crustaceans useful in terms of nutrition
  • daphnia is necessary to cultivate daphnia as a food organism containing a large amount of omega-3 polyunsaturated fatty acids, which are essential fatty acids.
  • Freshwater-concentrated chlorella and yeast developed and marketed for rotifers, the existing early food organisms, have a fatal weakness in that they lack omega-3 polyunsaturated fatty acids.
  • the low-density open-air culture method and high-density batch culture has problems with low productivity due to a low incubation rate and reduced survival rate despite feeding concentrated chlorella (freshwater, seawater), which is an expensive food. Because phosphorus has a thick cell wall, it is discharged without being completely digested, and the problem of water quality deterioration due to the generation of organic matter due to the accumulation of the cell wall and the decay of food, and commercial yeast, due to its own characteristics, water quality deterioration easily occurs. Fourth, in order to stably supply food organisms with seasonality and achieve industrialization, mass culture technology that can be produced year-round regardless of the season is required. (Samel et al., 1999, Ecotoxicology and Environmental Safety , 43: 103-110).
  • the present inventors to confirm the efficacy of the Cryptomonad cultured in fermentation culture tank Germany SAG; Chroomonas sp (progenitor number, 980-1) from (The Culture Collection of Algae at University Goettingen, Germany hereinafter referred to as SAG), Cryptomonas ovata (species number, 979-3) and Chilomonas paramecium (species number, 977-2a) were acquired and used in stock.
  • SAG Chroomonas sp (progenitor number, 980-1) from (The Culture Collection of Algae at University Goettingen, Germany hereinafter referred to as SAG), Cryptomonas ovata (species number, 979-3) and Chilomonas paramecium (species number, 977-2a) were acquired and used in stock.
  • SAG The Culture Collection of Algae at University Goettingen, Germany hereinafter referred to as SAG
  • Cryptomonas ovata
  • progenitor silver flagella were cultured using a 50 ml test tube, and the culture conditions were carried out by irradiating for 24 hours at a temperature of 25° C. and a 5,000-6,000 lx light source.
  • culture water was collected from the heterotrophic culture tank described in Example 6 of the present invention, and silver flagellum was collected under an optical stereoscopic microscope in a laboratory using a microtubule pipette. After looking at the shape and motility of the cells, one cell was isolated to obtain four types (Table 3).
  • the obtained four cultures were put in a 24-can culture plate using the same MiEB12 medium and ErbsS medium as above, and subcultured under the same temperature and light conditions as above and scale-up with a 50 ml test tube. It was used in the experiment (FIG. 2).
  • the composition of the MiEB12 medium is summarized in Table 1 below
  • the composition of the trace element solution is summarized in Table 2 below
  • the classification of silver flagella is summarized in Table 3 below.
  • composition of MiEB12 Medium Ingredients Stock solution (g/100 ml) Final concentration (ml) KNO 3 One 10 (NH 4 ) 2 HPO 4 0.2 5 MgSO 4 7H 2 O 0.1 10 CaSO 4 saturated solution 10 Soil Extract 3.1 20 peat moss extract 10 Trace element solution 5 Distilled water 930
  • composition of the trace element solution Ingredients Stock solution (g/100 ml) Final concentration (ml) ZnSO 4 7H 2 O 0.1 One MnSO 4 4H 2 O 0.1 2 H 3 BO 3 0.2 5 Co(NO 3 ) 2 6H 2 O 0.02 5 Na 2 MoO 4 ⁇ 2H 2 O 0.02 5 CuSO 4 ⁇ 5H 2 O 0.0005 One FeSO 4 7H 2 O 0.7g EDTA (Titriplex III, Merck) 0.8g Vitamin B12 (5 x 10 -6 g/l) - Distilled water 981
  • the present inventors performed a comparative experiment with the original species to find out the universal efficacy of self-sorted silver flagellum in a fermentation culture tank.
  • the control used in the experiment is Chroomonas sp. (Progenitor number, 980-1)
  • the experimental group were cultured using the medium MiEB12 intended for LF, LS, SF, SS of Table 3 Culture conditions Temperature 25 °C, 5,000 -6,000 lx light source was irradiated for 24 hours. After that, a 200 ml beaker was used for the culture vessel, and the initial inoculation concentration was 5x10 3 cells/ml, sampled daily, and cultured for 12 days.
  • the present inventors performed a comparative experiment for the same purpose as the first culture experiment.
  • the control group used in the experiment was Cryptomonas ovata (species species number, 979-3), and the experimental group was cultured in MiEB12 medium using LF, LS, SF, and SS, and the culture conditions were set the same as the first conditions.
  • the present inventors performed a comparative experiment for the same purpose as the first and second culture experiments.
  • the control group used for the experiment was Chilomonas paramecium (species number, 977-2a), and the experimental group used a slightly modified ErbsS medium for LF, LS, SF, and SS. did
  • the present inventors analyzed the omega-3 fatty acid content of silver flagellum. Specifically, total lipid extraction of silver flagellum, daphnia, and saltwater fish was performed with reference to the prior art (Bligh and Dyer, Can. J. Biochem. hysiol. 37: 911-917. 1995).
  • the sample was taken in a beaker and then pulverized at 15,000 rpm for 5 minutes in a cell grinder (homogenizer AM-12, Nihonseiki Kaisha Co. Ltd., Tokyo, Japan), followed by chloroform and ethanol 2:
  • the extraction solvent mixed with 1 was added at twice the amount of the sample and left for one day.
  • a funnel was placed on the round flask, and sodium sulfate (Na 2 SO 4 ) was added thereon to allow only the chloroform layer to slowly flow down. Then, the separated chloroform layer was completely evaporated at 40 ° C.
  • the present inventors performed heterotroph culture on the silver flagellum of the present invention. Specifically, 0.2N hydroxylation after adding 5-6 kg of an organic medium mixed with commercially available organic fertilizer and sterilized food waste water in a university cafeteria by half to 3 tons of culture water using a 4-ton fermentation tank (2m x 2m x 1m) Sodium (caustic soda) was added and the mixture was stirred at room temperature of 25-30° C. for 24 hours while raising the pH to 9 or higher. The next day, after neutralization to pH 6 by adding acetic acid, 1 ton of natural water was added to adjust the final organic medium content to 0.15% by weight to prepare an alkaline hydrolyzate. Cross-trophic culture was carried out for 12-15 days at room temperature of 25-30°C, under no light.
  • Example 7 Extraction and toxicity test of humic acid
  • the present inventors extracted humic acid from humus. Specifically, for the extraction of humic acid, the configuration of the apparatus was divided into an extraction unit, an adjustment unit, an air stripping unit, and a storage tank, and a 1-ton acrylic cylinder was used (FIG. 6). After adding 1 ton of water to the extract, 1 kg of peat moss was added, put into a mesh 52, and suspended and insoluble substances were removed. Water was stirred in the lower part of the extraction part by aeration, and circulating water was continuously dropped from the upper part of the extraction part, and humic acid was effectively eluted from Humus by strong water flow stirring.
  • control unit controls the amount of water entering the air stripping unit through the circulation pump and bypass, and effectively removes dissolved organic matter through the skimmer effect, and the stagnation time of the extract water in the air strip unit is set to 3 minutes.
  • the extraction conditions were 25° C., pH 8.0, and dissolved oxygen was extracted for 5 days in a saturated state to prepare a stock solution of humic acid.
  • the present inventors conducted a toxicity test in order to prevent this because mortality frequently occurs when the water-soluble humic acid stock solution prepared in 7-1 is used directly to daphnia.
  • the culture vessel was filled with 30 ml of culture solution using a 40 ml glass sample bottle, and then mounted on a sample bottle rack. After that, one experimental group was designed to have 10 repetitions using 10 sample bottles. After filling the sample with a certain amount, one daphnia was put into each sample bottle. The lifespan until death was defined as the lifespan of one generation, the number of daphnia larvae produced during that period was called the number of births, and the number of larvae produced by one larvae was called the number of live births ( ⁇ ). .
  • the present inventors verified the efficacy of the humic acid prepared in 7-1 above.
  • phytoplankton such as chlorella
  • the efficacy of humic acid on the survival of daphnia was verified because it frequently occurred empirically that the occasional injection of Humus prolongs survival.
  • the experimental method was carried out in the same way as the humic acid toxicity test, and commercial freshwater concentrated chlorella was used to feed daphnia.
  • the daphnia used in the experiment was subjected to a preliminary experiment in advance, acclimatized to the experimental food, and then the same embryonic caterpillars obtained from one mother daphnia were selected as the experimental daphnia.
  • the experimental mother daphnia was transferred to a sample bottle containing fresh food every 24 hours, and then the survival period, the number of spawning times, the number of spawning intervals, and the total number of live eggs were measured.
  • the present inventors performed mass culture of Daphnia using the culture system of the present invention. Specifically, a heating device, an oxygen supply device, a water quality measurement device, a food supply device, and a harvest device were installed in a 20-ton rectangular prefabricated canvas tank (7m x 5m x 1m) (FIG. 7).
  • the culture conditions were water temperature 25°C, illuminance of 1,000 lx, photoperiod of 14L : 10D, and the initial density was adjusted to 0.01 ⁇ 0.001 ind./mL. DO measurement was measured using YSI-5000 (YSI, Co., Ltd., USA), and oxygen control was maintained in an appropriate amount using a blower and airstone (Table 7).
  • pH was measured using pH20N (ISTEK, Co., Ltd., Korea), and several 1 kW heaters (Ami, Co., Ltd., Korea) were used to maintain the water temperature, and a temperature controller (801-H, Ami, Co., Ltd., Korea) was used for adjustment.
  • a 50 mm PVC pipe was installed on the upper layer of the water tank to collect daphnia, and a valve was installed to control the amount of water during drainage.
  • concentrated silver flagellate algae were fed with a metering pump (FRJ800PFX5, Wilo, Co., Ltd., Korea) and maintained at 7°C using a small refrigerator to maintain freshness.
  • the present inventors attempted an indoor demonstration experiment to verify the field application system connected to the seed culture tank among the Daphnia mass culture system (FIG. 9). Specifically, it consisted of a 1 ton daphnia culture tank (3m x 0.5m x 1m), a 1 ton seed culture tank (3m x 1m x 0.4m), a food quantity supply device, a heating device, and a water quality measuring device. In addition, a certain amount of refrigerated concentrated silver flagellum was supplied to the daphnia culture tank by using a metering pump. The dilution ratio of humic acid supplied to the daphnia culture tank was 10%.
  • the cultured daphnia was supplied to the seed culture tank as a drop by a specified circulation rate of supplemental water (FIG. 10).
  • the fertilized hyangfish eggs used in this experiment were purchased from Seongdeok Fish Farm in Seongdeok-dong, Deokjin-gu, Jeonju-si, Jeollanam-do (Table 9).
  • daphnia production results size at time of inoculation (Hatcher, Marie) size at the time of release (length cm, weight g) incense fish (number of animals) survival rate (%) 5,000 3 cm (1 g) 3,000 60
  • the present inventors verified the effect of daphnia produced according to an embodiment of the present invention on saltwater fish.
  • the black sea bream used in the experiment carried eggs directly from the Suhwa Fisheries nursery in Sansam-myeon, Goseong-gun, Gyeongsangnam-do, and then hatched and finished feeding the worms.
  • fry hatched from eggs were transported, but as soon as they arrived at the laboratory after transport, all died and changed to a method of transporting fertilized eggs.
  • the experimental design was designed as a daphnia group and an artemia group, and 100 animals in each experimental group were performed in 3 repetitions.
  • the breeding device is a 60 cm ⁇ 30 cm ⁇ 30 cm square water tank, with a water volume of 30 L, and is a circulation filtration system so that excrement can be immediately discharged out of the breeding tank by the continuous addition of filtered seawater.
  • the water supply was adjusted to 1 L/min and the air flow to 4.5 L/min, and the water temperature throughout the breeding period was maintained at 25 ⁇ 1 °C, and each food was always maintained at least one per mL.
  • the experiment was conducted for 6 weeks, and measurements were made at 2-week intervals for all subjects.
  • the Artemia group grew from 0.2 g to 1.12 g after 6 weeks, whereas the Daphnia group grew from 0.2 g to 1.61, and the daphnia group exhibited a very high growth rate of 8 times compared to 5.6 times of the Artemia group (Fig. 11). ).
  • the above results suggest that the daphnia cultured with the concentrated silver flagellum of the present invention can replace the conventional artemia as food for black sea bream fry.
  • the method for mass culturing daphnia using concentrated silver flagellum of the present invention solves the problem of essential fatty acid nutrient deficiency in the existing daphnia feed, and can solve the problems of self-contamination and contamination of epiphytes in culture water that occur during high-density culture with conventional food. have.
  • by solving the problem of lowering the reproduction rate and growth rate of conventional daphnia it has the effect of remarkably extending the lifespan of daphnia, and it has the effect of being able to plan daphnia production by solving the cause of daphnia mortality that occurs frequently in fresh water.

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Abstract

La présente invention concerne un procédé de culture de masse de cladocères à l'aide de cryptophytes, le procédé permettant la culture à haute densité de cladocères par la production efficace de cryptophytes concentrées contenant une grande quantité d'acide gras oméga-3 polyinsaturé, par l'application d'une culture hétérotrophe dans un milieu organique en l'absence de lumière.
PCT/KR2021/007454 2020-06-16 2021-06-15 Procédé de culture de masse de cladocères à l'aide de cryptophytes WO2021256821A1 (fr)

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Citations (5)

* Cited by examiner, † Cited by third party
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KR100426791B1 (ko) * 2001-12-22 2004-04-14 학교법인조선대학교 조류 및 물벼룩을 이용한 생태학적 유기성 폐수처리 방법
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