WO2021151366A1

WO2021151366A1 - Health food or traditional chinese medicine medicinal formula for improving male sexual function, and ultrasonic wave preparation method

Info

Publication number: WO2021151366A1
Application number: PCT/CN2021/072604
Authority: WO
Inventors: 任晓锋
Original assignee: 江苏大学
Priority date: 2020-02-02
Filing date: 2021-01-19
Publication date: 2021-08-05
Also published as: CN111228414A

Abstract

A traditional Chinese medicine composition or health food for improving male sexual function, and an ultrasonic wave preparation method, with said composition or health food being prepared using traditional Chinese medicine ingredients such as rhizoma curculiginis, herba epimedii, cortex eucommiae, radix morindae officinalis, radix astragali, radix angelicae sinensis, radix achyranthis bidentatae, radix codonopsis, fried rhizoma atractylodis macrocephalae, poria, ramulus cinnamomi, fried radix paeoniae alba, semen cuscutae, deerhorn pieces, Huai wheat, radix glycyrrhizae, tangerine peel, fructus lycii, and radix rehmanniae preparata, and the preparation method being to comminute the traditional Chinese medicine ingredients and then use an ultrasonic wave extraction apparatus to perform extraction on respective ingredients individually or mixed together, extracting liquid concentrates, and performing drying. The present invention is able to shorten mating latency in mice and increase mating frequency within a 20 minute time period, and also increases the weight of accessory sex organs in male mice and increases nitric oxide (NO) and testosterone concentrations in the serum of male mice.

Description

Health food or traditional Chinese medicine compound for improving male sexual function and ultrasonic preparation method

Technical field

The invention relates to the technical field of traditional Chinese medicine compound preparations or health foods, in particular to a traditional Chinese medicine composition or health food for improving male sexual function and anti-fatigue, and an ultrasonic preparation method.

Background technique

In recent years, with the continuous development of society and economy, mankind’s requirements for the quality of life have become higher and higher, the pace of life has been accelerated, the pressure of life has increased, and environmental pollution has increased. Male reproductive health problems have become more and more prominent. Some of the diseases and unhealthy habits, such as prostatitis, smoking and alcoholism, lack of exercise, and drug use, have led to a significant increase in male sexual dysfunction. Male sexual dysfunction refers to the impotence of male sexual function and sexual satisfaction, often manifested as sexual desire disorder, impotence, premature ejaculation, nocturnal emission, non-ejaculation and retrograde ejaculation. Sexual behavior is not only instinctive, but also a physiological activity based on mental and psychological activities. Therefore, male sexual dysfunction is partly caused by organic diseases such as systemic diseases and reproductive system diseases. Sexual dysfunction not only brings psychological pressure to patients, but also directly affects the quality of life of patients, which may affect the treatment of the primary disease. Therefore, it should attract clinical attention (Drug-induced male sexual dysfunction, Chinese Journal of Hospital Pharmacy, 2001, 21st Volume 12).

In addition to male sexual dysfunction, semen quality is also an important part of male reproductive skills, and male infertility caused by abnormal semen accounts for about 70% to 80%. Dyspermic infertility includes six symptoms: azoospermia, oligospermia, dead sperm, low sperm motility and non-liquefaction of sperm, and sperm deformity. The reasons include abnormal testicular development, chromosomal abnormalities, spermatogenesis dysfunction and local disease (recessive varicocele, huge hydrocele and other diseases).

At present, Western medicine has good results for a clear cause or for a single diseased tissue, but most people cannot find the direct cause, so they need to nurse for supplementary treatment. Western medicine is more commonly used to treat sexual dysfunction, and the effect is better than that of traditional Chinese medicine. However, the side effects of Western medicine are relatively large, such as cardiovascular events, visual disturbances, dizziness, facial flushing, nausea, etc. Traditional Chinese medicine is the most commonly used to treat sexual dysfunction. Traditional Chinese medicine has a good pharmacological effect. At present, traditional Chinese medicine is basically a method of strengthening yang and nourishing the kidney and strengthening the essence. There are no toxic side effects. At present, although there have been drugs or formulations that improve male sexual function and semen quality, due to the diverse components of traditional Chinese medicine compounds, the current preparation method is only hot water or ethanol extraction, and it is not possible to extract all the active ingredients in traditional Chinese medicine. It is extracted, which results in low efficiency of traditional Chinese medicine compound preparations, or requires a longer time to take the medicine. Therefore, in addition to researching suitable Chinese medicine compound accidents, it is necessary to develop corresponding high-efficiency green preparation methods.

Ultrasound is a sound wave with a frequency of 2×10 ⁴ Hz～10 ⁹ Hz. It is the propagation form of the mechanical vibration state (or energy) of an object. Its propagation frequency is high, the wavelength is short, the propagation specificity is strong, and the medium vibration intensity is the main reason. Features. Now generally according to the use of ultrasound, it is divided into two major types of ultrasound: detection ultrasound and power ultrasound. Power ultrasound is a technology that uses ultrasonic vibration energy to change or accelerate the physical state, chemical properties, biological characteristics and functions of materials, and is widely used Industry, agriculture and medicine. At present, the application of ultrasonic processing technology in the processing of traditional Chinese medicine is mainly to promote the extraction of active ingredients in organisms. It has unique advantages in promoting the extraction of active ingredients of Chinese medicinal materials. The extraction of active substances, etc., the application of ultrasonic processing technology has improved the extraction efficiency.

Summary of the invention

The purpose of the present invention is to make up for the deficiencies of the prior art, and provide a scientific formula, safe and reliable traditional Chinese medicine composition, health food and ultrasonic preparation method that can effectively improve sperm quality and resist fatigue.

The technical scheme adopted by the above-mentioned traditional Chinese medicine composition is as follows:

A Chinese medicinal composition for improving male sexual function and anti-fatigue, prepared from the following Chinese medicinal materials in parts by weight:

The Xianling Spleen (10-20 parts) in the above-mentioned Chinese medicinal materials can also be replaced with Cynomorium (10-20 parts).

In the above-mentioned Chinese medicinal materials, Xianling Spleen (10-20 parts) can also be replaced by Ziheche (10-20 parts), and Lujiao tablets (10-20 parts) can be replaced by tortoise shell tablets (10-20 parts).

The eucommia ulmoides mentioned is fried eucommia ulmoides, and the raw eucommia ulmoides is sprayed with salt water and mixed well, and when it is exhausted, the eucommia ulmoides is sautéed with a slow fire until it is yellow-brown and used as medicine.

The said astragalus is honey roasted astragalus, and the astragalus slices are mixed well with honey, and when they are not sticky, they are taken out and aired, and then used as medicine.

The licorice described therein is lucid licorice.

A Chinese medicinal composition for improving male sexual function and anti-fatigue is preferably prepared from the following raw materials in parts by weight:

In the above-mentioned Chinese medicinal materials, Xianlingpi (15 parts) can also be replaced with Cynomorium (15 parts).

In the above-mentioned Chinese medicinal materials, Xianling Spleen (15 parts) can also be replaced by Ziheche (15 parts), and Lujiao tablets (15 parts) can be replaced by tortoise shell tablets (15 parts).

The method for preparing the above-mentioned traditional Chinese medicine composition for improving male sexual function and anti-fatigue is carried out according to the following steps:

(1) The above-mentioned raw materials are respectively pulverized in parts by mass, passed through a sieve of 80-100 mesh, and then thoroughly mixed.

(2) Add tap water or mineral water at 1:10-20 (m/v), and use a multi-mode ultrasonic extractor for extraction at a temperature of 60-70°C. After the extraction is finished, pour the supernatant and add the same amount of tap water or mineral water, and repeat the extraction process for 1 round. The combined extracts are filtered to remove impurities, and the filtrate is vacuum concentrated and freeze-dried to prepare a traditional Chinese medicine composition.

Wherein, in addition to tap water or mineral water, the extraction solvent described in step (2) can also be an ethanol-water solution with a volume fraction of 40-70%.

The specific parameters of the multi-mode ultrasonic extraction in step (2) are ultrasonic time 10min-40min, preferably ultrasonic time 30min; ultrasonic power 60W/L-180W/L, preferably ultrasonic power 150W; ultrasonic frequency 20kHz, 28kHz, 35kHz , 40kHz, 60kHz, or a combination of two or two, or a combination of three or three, preferably the ultrasonic frequency 35kHz/60kHz or 20kHz/35kHz/60kHz.

The method for preparing the above-mentioned traditional Chinese medicine composition for improving male sexual function and anti-fatigue may be carried out according to the following steps:

(1) After pulverizing the above raw materials in parts by mass and passing through a sieve of 80-100 mesh,

(2) Each raw material is added to tap water or mineral water at a ratio of 1:10-20 (m/v), and the multi-mode ultrasonic extractor is used for extraction at a temperature of 60-70°C. After the extraction, pour the supernatant and add the same amount of tap water or mineral water, repeat the extraction process for 1 round; combine the extracts to filter and remove impurities, and the filtrate is vacuum concentrated, spray dried or freeze-dried to obtain a single Chinese medicine The extract is then mixed according to the ratio of the above-mentioned raw materials to obtain a traditional Chinese medicine composition.

The product of the traditional Chinese medicine composition for improving male sexual function and anti-fatigue of the present invention can be solid beverage powder, oral liquid, pill, tablet, powder, granule, capsule, pill or decoction.

A health food for improving male sexual function and anti-fatigue, prepared from the following parts by weight of Chinese medicinal materials:

The said astragalus is honey-scorched astragalus, which is the astragalus slices mixed with honey, and when the astragalus slices are not sticky, the astragalus is taken out and aired, and then used as medicine.

The licorice described therein is lucid licorice.

A health food for improving male sexual function and anti-fatigue, preferably prepared from the following parts by weight of raw materials:

The method for preparing the above-mentioned health food for improving male sexual function and anti-fatigue is carried out according to the following steps:

(2) Add tap water or mineral water at 1:10-20 (m/v), and use a multi-mode ultrasonic extractor for extraction at a temperature of 60-70°C. After the extraction is finished, pour the supernatant and add the same amount of tap water or mineral water, and repeat the extraction process for 1 round. The combined extracts are filtered to remove impurities, and the filtrate is vacuum concentrated, spray-dried or freeze-dried to prepare a traditional Chinese medicine composition.

The method for preparing the above-mentioned health food for improving male sexual function and anti-fatigue may be carried out according to the following steps:

The health food for improving male sexual function and anti-fatigue of the present invention can be solid beverage powder, oral liquid, pill, tablet, powder, granule, pill, capsule or decoction.

The efficacy of each component of the main traditional Chinese medicine components of the present invention is as follows:

Cuscuta, Chinese wolfberry, Poria, as raw materials of the same medicine and food, have the functions of strengthening bones and muscles, promoting blood circulation, invigorating the middle and nourishing qi, replenishing kidney and reducing urine, nourishing vitality and yang, and nourishing liver and eyesight. Among them, Cuscuta and Lycium barbarum are important components of Wuzi Yanzong Pill, an ancient prescription for the treatment of infertility. They have the effect of treating impotence, infertility, spermatorrhea, premature ejaculation and low back pain caused by the deficiency of kidney essence.

Curculigo (scientific name: Curculigo orchioides Gaertn.), also known as ground palm (Sichuan, Guizhou), Duplexia (Sichuan), Codonopsis (Fujian), Curculigo (Yunnan), Hainan ginseng (Hainan), Maozhaozi, Brahman Ginseng belongs to Curculioceae, and the flowering period is from April to September. Warm kidney and yang; remove cold and dampness. Main impotence and coldness; urinary incontinence; cold pain in the abdominal abdomen; pain in the waist and knees; weak muscles and bones; spasms of lower limbs; menopausal syndrome.

Xianling spleen is also called Gangqian, Epimedium, Xianlingpi, Fangcao, Qianliangjin, Dried Chicken Tendon, Coptis chinensis, Three Branches and Nine Leaves, etc. The whole plant of Epimedium sagittatum (Sieb.et Zucc.) Maxim. or Epimedium E.brevicornum Maxim. It has the effects of invigorating the kidney and strengthening yang, dispelling wind and removing dampness, strengthening muscles and bones. Xianling Spleen can treat impotence and nocturnal emission, deficiency and cold infertility, frequent urinary incontinence, kidney deficiency, cough, waist and knee weakness, rheumatic arthralgia, hemiplegia, and insensitive limbs.

The functions of Eucommia (scientific name: Eucommia ulmoides Oliver) are: invigorating the liver and kidney, strengthening the bones and muscles, and restoring fetus. Cure waist and knee soreness, weak feet and knees, urinary drain, wet itching under the vagina, fetal leakage, restless fetal movement, high blood pressure. Generally, the effect of single Chinese medicine is not obvious, and it is used in combination. Therapeutic effects of Eucommia ulmoides: The taste is sweet, slightly pungent, and warm in nature. Return to the liver and kidney channels. Qi and run down. It can nourish the liver and kidney, strengthen the bones and muscles, and relieve the fetus. Indications of waist and knee pain, impotence, spermatorrhea, frequent urination, dyspnea, yang hyperactivity, dizziness, rheumatism arthralgia, dampness and itching under the vagina, fetal movement, missed fetus and miscarriage. Raw eucommia: Wash the original medicine, brush off the rough skin, separate the thickness, and cut into wide strips to dry. The main difference from the cooked eucommia is that the surface is light brown, smoother, the inner surface is smooth, and the texture is brittle and easy to break. There are silver-white filaments connected together, dense, slightly stretchable, and light in taste; it has a good effect on lowering blood pressure. Cooked eucommia: It means to fry eucommia ulmoides. For raw eucommia ulmoides, sprinkle it with salt water and mix well, wait until it is exhausted, and then stir-fry it with a slow fire until it is yellow-brown and used as medicine. The main difference with raw eucommia is that the surface color is darker, there are burnt spots, the cross-section has silvery white silky material that is easy to break, almost no elasticity, and the taste is salty; cooked eucommia is fried with salt, and it is said that the kidney can be used to invigorate the kidney. The effect is better. In the present invention, Eucommia ulmoides is fried Eucommia ulmoides.

Morinda officinalis (scientific name: Morinda officinalis How) is mainly produced in Guangdong, Guangxi and other places. It is mainly used to treat impotence and nocturnal emission, cold uterus and infertility, irregular menstruation, cold pain in the abdomen, arthralgia due to rheumatism, and weakness of muscles and bones. (It belongs to the list of Chinese medicines that can be used in health foods announced by the Health and Family Planning Commission)

Astragalus is the root of Astragalus, and Astragalus (scientific name: Astragalus propinquus Schischkin) is a plant of the genus Astragalus in the leguminous family. Pus, growth of muscle, strengthening of capillary resistance, antiperspirant and sex hormone-like effects, treatment of superficial deficiency and spontaneous perspiration, Qi deficiency internal injury, spleen deficiency diarrhea, edema and carbuncle, etc. The present invention uses honey-dried astragalus, also known as honey-dried astragalus or sun-dried astragalus. That is, the astragalus slices are mixed well with honey, and when they are not sticky, they are taken out and aired, and then used as medicine. Honey Astragalus is generally round or oval slices, with honey aroma, sweet taste and slightly sticky. It will have a slight beany taste when chewed in the mouth. After being fried with honey, it enhances the qi-tonifying and nourishing lung effects of Astragalus. The efficacy and role of honey astragalus is roughly the same as that of astragalus. Therefore, honey astragalus can replenish qi and nourish the middle energy, be used for qi deficiency and fatigue, and loose stools when eating less. Invigorate the fire and help the yang, lead the fire back to the source, dispel cold and relieve pain, and promote blood circulation to promote menstruation. It is suitable for impotence, cold uterus, cold pain in the heart and abdomen, vomiting and diarrhea due to deficiency and cold, amenorrhea, dysmenorrhea, warming the meridian and improving the pulse.

Angelica is the dried root of Angelica sinensis (Oliv,) Diels. Excavate at the end of autumn to remove the fibrous roots and silt. After the water evaporates, they are tied into small handfuls, put on the shed, and slowly smoked dry with fireworks. The root of the whole angelica is slightly cylindrical, the upper end of the root is called "guitou", and the main root is called "guishen" or "cunshen". The roots are called "Guiwei" or "Guigui", and all are called "Quangui". It can nourish blood; promote blood circulation; regulate menstruation and relieve pain; moisturize dryness and smooth intestines. Main syndromes of blood deficiency; irregular menstruation; amenorrhea; dysmenorrhea; agglomeration of symptoms; uterine bleeding; abdominal pain of deficiency and cold; paralysis of atrophy; numbness of the skin; effect.

Achyranthes alias: Achyranthes bidentata Blume, Latin name: Achyranthes bidentata Blume. Amaranthaceae, Achyranthes is a perennial herb. The root is used as medicine, and it is used in health to promote blood circulation and promote menstruation; treat postpartum abdominal pain, irregular menstruation, amenorrhea, epistaxis, fire, toothache, beriberi edema; regular use, nourish liver and kidney, strengthen waist and knees; cure waist and knee pain, liver and kidney deficiency, Bruises and bruises. Both Huai Achyranthes bidentata or Sichuan Achyranthes bidentata in the present invention can achieve the effect.

Codonopsis (scientific name; Codonopsis pilosula (Franch.) Nannf.) is a genus of Codonopsis of Campanulaceae, a perennial herb with milk. The base of the stem has many tumor-like stem scars, and the roots are often hypertrophic and have a spindle-shaped or spindle-shaped cylindrical shape. Codonopsis is a traditional tonic medicine commonly used in China. In ancient times, Codonopsis from the Shangdang area of Shanxi was used as the top grade. It has the effects of nourishing the middle and nourishing qi, strengthening the spleen and lungs. Codonopsis has the functions of enhancing immunity, expanding blood vessels, lowering blood pressure, improving microcirculation, and enhancing hematopoietic function. In addition, it can improve the white blood cell decline caused by chemotherapy and radiotherapy.

Atractylodes macrocephala (scientific name: Atractylodes macrocephala Koidz.): Atractylodes macrocephala is a perennial herbaceous plant in the Compositae family, up to 60 cm tall, with nodular rhizomes. Atractylodes is bitter, sweet, and warm. To invigorate the spleen and replenish qi, dry dampness and diuresis, antiperspirant, anti-fetus. Used for spleen deficiency, lack of food, indigestion, diarrhea, edema, spontaneous sweating, and fetal movement. Stir-fried Atractylodes macrocephala is a tonic medicine that has the effects of invigorating the spleen and qi. It is to sprinkle the honey-roasted bran into a hot pot. When it smokes, add Atractylodes macrocephala flakes. Go honey bran. For every 100kg of Baizhu tablets, 10kg of bran is roasted with honey.

Poria cocos (Schw.) Wolf is the dried sclerotia of Poria cocos (Schw.) Wolf. Excavated more than July to September. After excavation, the sediment is removed. After piled up "sweat", spread out and dry until the surface is dry, then "sweat", repeat several times until wrinkles appear and most of the internal moisture is lost, then dry in the shade , Called "Fu Ling Ge"; or cut fresh Poria according to different parts, dried in the shade, respectively called "Fu Ling block" and "Fu Ling piece". It has the effects of diuresis and dampness, invigorating the spleen and calming the heart. Used for edema, oliguria, dizziness, spleen deficiency, lack of food, loose stools, diarrhea, restlessness, palpitations, and insomnia.

Osmanthus cassia, belongs to the order of Cinnamomum, and is a medium-large tree of Lauraceae. Cut the twigs in spring and summer, dry them in the sun or in the shade, and cut them into thin slices or small pieces for use. Xin, Gan, and warm. Guixin, lung, bladder meridian. It has the effects of sweating and relieving muscles, warming the meridians, promoting yang and transforming qi, and calming and lowering qi. Commonly used for cold, cold, abdominal pain, blood-cold amenorrhea, arthralgia, phlegm, edema, palpitations, etc.

White peony (scientific name: Paeonia lactiflora Pall) is also called white peony, and it is a plant of the genus Paeonia in the family Paeoniaceae. It has a long history of cultivation in China and is well-known both at home and abroad. It is called white peony. Perennial herb or subshrub, born in shrubs or grasses on hillsides and valleys. It is planted in most areas of our country. The effects of white peony: nourish blood and soften the liver, relieve pain, restrain the yin and reduce sweat. Cure chest and abdomen flank pain, diarrhea, abdominal pain, spontaneous sweating, night sweats, yin deficiency and fever, irregular menstruation, metrorrhagia, and vaginal discharge. Fried white peony is a kind of medicine. It is the dried root of PaeonialactifloraPall., a plant of the Ranunculaceae family. The surface is white or light reddish-brown, calms the liver and relieves pain, nourishes blood and regulates menstruation, suppresses yin and relieves sweat. Used for headache, dizziness, hypochondriac pain, abdominal pain, limb contracture, blood deficiency and chlorosis, irregular menstruation, spontaneous sweating, night sweats, from the "Chinese Pharmacopoeia".

Cuscuta chinensis Lam. (scientific name: Cuscuta chinensis Lam.), aliases such as Zen Zhen, Douyi, Dou Yan, Huang Si, Huang Si Teng, Jin Si Teng, etc. Annual parasitic herb. Cuscuta is a good medicine for tonifying the kidney, liver, and spleen. It is mainly used clinically for kidney deficiency, low back pain, impotence and nocturnal emission, frequent urination, uterine cold, infertility, dark eyes and loose stools. Cuscuta is sweet and warm, returning to the kidney, liver, and spleen meridians. It has the effects of nourishing liver and kidney, consolidating urea and reducing urine, restoring fetus, improving eyesight, and stopping diarrhea. Sweetness generally has a nourishing effect. The sweetness is slightly warm, and the energy is neutralized. It can not only nourish yang, but also replenish yin. It has the characteristics of warm but not dry, nourishing but not stagnant. Gujing anti-fetus and sex hormone-like effect.

Lycium barbarum is the mature fruit of the Solanaceae plant Lycium barbarum. Pick the fruits when they are ripe in summer and autumn, remove the stalks, dry them in a cool place until the skins are wrinkled, and then expose them to the sun until the skins are dry and hard and the flesh is soft. Lycium barbarum, also known as Lycium barbarum and red earrings, is the mature fruit of Lycium barbarum, a small shrub of the Solanaceae family. Chinese wolfberry has a long history of homologous medicine and food. It is a well-known precious Chinese medicinal material in China and abroad. The effect of the old, also known as "but Laozi". Lycium barbarum contains a variety of amino acids, and contains special nutrients such as betaine, zeaxanthin, and berry red, which make it have a very good health effect. Suitable for everyone. It is more suitable for people with excessive eyes and the elderly. Goji berries are relatively mild in nature, and eating a little too much is not harmful, but if there is no restraint, eating too much can make you angry.

Lujiao tablets have the effects of nourishing kidney yang, nourishing essence and blood, strengthening muscles and bones, promoting blood flow and reducing swelling. Symptoms such as stasis and swelling, muscle and bone pain.

Huai wheat is called Huai wheat because it is mainly produced in Jianghuai area. Sweet in taste, cool in nature, capable of restraining sweat, deodorizing, replenishing qi, nourishing the heart, and removing heat. It can be used whether it is spontaneous sweating due to yang deficiency or night sweating due to yin deficiency. Huai wheat can also nourish qi and nourish yin and remove heat, and it has certain therapeutic effects on symptoms of yin deficiency and fever and bone steaming fever. Huai wheat can nourish the human body's innate kidneys, and can also nourish the acquired spleen and stomach, and can invigorate the spleen, stomach and kidney. It is a good health food.

Licorice (scientific name: Glycyrrhiza uralensis Fisch) aliases: Guo Lao, Sweet Grass, Ural Licorice, Sweet Root Seed. Leguminous and licorice are perennial herbs with strong roots and rhizomes. They are a tonic Chinese herbal medicine. The medicinal parts are roots and rhizomes, which have the effects of invigorating the spleen and qi, clearing away heat and detoxification, eliminating phlegm and relieving cough, relieving emergency and relieving pain, and reconciling various drugs. It is often used for spleen and stomach weakness, fatigue, palpitations, shortness of breath, coughing and excessive sputum, abdominal and extremity contractures, acute pain, carbuncle swelling and sore, to relieve drug toxicity and potency.

Roasted licorice is licorice roasted with honey, commonly known as concoction, which is to make honey in a pot to make medium honey, then use a slow fire to add raw licorice slices and stir fry evenly, take out the pot for 3 to 5 minutes, and bake it in a roasting room or oven at 60°C. Take it out when it is not sticky and let it cool. Roasted licorice is similar to round or oval slices, the surface is reddish-brown or gray-brown, slightly shiny, the cut surface is yellow to dark yellow, the cambium ring is obvious, and the rays are radial. Zhigancao Decoction has the therapeutic effects of replenishing qi, nourishing yin, and tonifying yang and rejuvenating the pulse.

Chenpi is the dried and mature peel of the Rutaceae plant Citrus reticulata Blanco and its cultivated varieties. Pick ripe fruit, peel off the skin, and dry or dry at low temperature. Regulate qi and invigorate the spleen, dry dampness and resolve phlegm. It is used for full abdominal distension, vomiting and diarrhea with less food, coughing and more sputum.

Rehmannia glutinosa (Latin scientific name: Rehmannia glutinosa (Gaetn.) Libosch.ex Fisch.et Mey.), a perennial herbaceous plant belonging to the genus Scrophulariaceae, up to 30 cm tall, fleshy rhizomes, fresh yellow, purple stems under cultivation conditions Red. The diameter can reach 5.5 cm, the leaves are ovate to oblong, the veins are concave on the top, and the flowers are arranged in a raceme at the top of the stem. The outer corolla is purple-red and the inner yellow-purple, the medicine chamber is rectangular, and the capsule is ovate to long. Oval, flowering and fruiting period from April to July. Rehmannia glutinosa is the rhizome of Rehmannia glutinosa or Rehmannia glutinosa of the Scrophulariaceae family, which is made by steaming and drying. The functions and indications are: nourishing yin and nourishing blood. Cure less blood due to yin deficiency, weak waist and knees, fatigue, steaming, nocturnal emission, metrorrhagia, irregular menstruation, diminished thirst, dryness, and deafness.

Cynomorium (scientific name: Cynomorium songaricum Rupr.), Cynomorium, Cynomorium is a perennial fleshy parasitic herb, without chlorophyll, the whole plant is reddish brown, most of which are buried in sand. Cynomorium buds of different sizes grow on the roots of parasitic roots, which are nearly spherical at the beginning, and then become elliptical or long cylindrical, with many fibrous roots and shed scale leaves. The stems are cylindrical, upright and brown. The stems buried in the sand have small fibrous roots, and the base of the stem is slightly thickened or enlarged. The fleshy stems removed from the inflorescence are used for medicinal purposes (medicinal name: Cynomorium), can nourish the kidneys, nourish essence, moisturize dryness, cure impotence and nocturnal emission, weak waist and knees, dry intestines and constipation, and have a certain effect on paralysis and improve sexual weakness.

Ziheche refers to the placenta of human beings. Chinese medicine believes that the placenta is sweet, salty and warm in nature. Modern medical research believes that the placenta contains protein, sugar, calcium, vitamins, immune factors, female hormones, progesterone, steroid hormones, gonadotropins, corticotropin, etc., which can promote the development of breast, uterus, vagina, and testicles. It also has a stimulating effect on the thyroid, and also has a good effect on tuberculosis, bronchial asthma, anemia, etc.

The tortoise shell piece comes from the back and plastron of the tortoise Chinemys reevesii (Gray). It can be harvested throughout the year, most of which are captured after August, and executed after capture. The muscles are removed, the nails are removed, washed and dried, and called "blood plates"; the nails are boiled and called "hot plates". The carapace is long oval and arched. The outer surface is brown or dark brown, with 3 dorsal ribs, 1 neck shield, narrow front and wide rear, 5 vertebral shields, symmetrical rib shields, 4 on each side, 11 marginal shields on each side, and 2 hip shields. The ventral deck is sheet-shaped, nearly rectangular and elliptical, and is formed by symmetrically fitting 12 gussets. The appearance is yellowish-brown or brown, with purple-brown radial markings. The inner surface is yellowish-white to off-white, and some are slightly bloody or residual flesh. Hard quality. The smell is slightly fishy and the taste is slightly salty. Slightly cold in nature, salty and sweet in taste. Return to the liver meridian, kidney meridian and heart meridian. It has the functions of nourishing yin and yang, nourishing the kidney and strengthening the bones, nourishing blood and nourishing the heart. It is a yin tonic drug under the classification of tonic drugs.

The beneficial effects of the present invention are:

(1) Aiming at the shortcomings of the existing medicines or Chinese medicine compounds for improving the quality of male sperm, a Chinese medicine compound composition and health food formula which can effectively improve the quality of sperm and improve the sub-health condition are proposed with scientific compatibility and high efficacy.

(2) The physical method used in the process of extracting traditional Chinese medicine in the present invention is ultrasonic treatment. Ultrasound is a green and environmentally friendly physical processing method. It is widely used in natural product extraction and food industry. It is a new type of physical processing method for preparing Chinese medicine compound extracts; the extraction rate is increased by 15.1% to 50.9%, and the time is shortened by about 95% .

(3) On the basis of maintaining the effect of improving sperm quality, the present invention utilizes the synergistic effect of different traditional Chinese medicines or dual-purpose active ingredients of medicine and food to improve the sub-health condition of the male body. Animal experiments in mice show that the formula of the present invention can shorten the incubation period of mice mating, increase the number of mating within 20 minutes, and can significantly increase the weight of the accessory organs of male mice, and increase the serum nitric oxide (NO) and testosterone of male mice. Concentration, the formula of the present invention has a significant effect on improving sexual function.

Description of the drawings

Figure 1 is the structure diagram of the multi-mode ultrasonic biological treatment equipment of the present invention, in which 1, 2, 3 are ultrasonic vibration plates, 4 is a liquid container, 5 is a water bath, 6 is a temperature probe, 7 is a circulating pump, and 8 is a computer Program controller, 9, 10, 11 are ultrasonic controllers.

Detailed ways

Unless otherwise specified, the terms used in the present invention generally have the meanings commonly understood by those of ordinary skill in the art. The present invention will be described in further detail below in conjunction with specific embodiments and with reference to data. It should be understood that these embodiments are only a further description of the present invention, and should not be construed as limiting the scope of protection of the present invention. Technical engineers in this field can make some non-essential improvements and adjustments to the present invention based on the content of the above-mentioned invention.

Figure 1 is the multi-mode ultrasonic biological treatment equipment of the present invention. The equipment is equipped with a computer program controller 8, which can set ultrasonic working parameters (ultrasonic power density, frequency, pulse working time, intermittent time and total treatment time) to be controlled separately Three

ultrasonic controllers

9, 10, 11 are respectively connected to three ultrasonic vibration plates 1, 2, 3 of different frequencies, which can realize single frequency/two frequency/three frequency ultrasonic treatment; put the solution to be processed into the liquid Single-frequency/dual-frequency/multi-frequency ultrasonic treatment is performed in the device 4, and the circulating pump 7 is started to circulate the solution. The automatic control of the temperature of the solution is realized by the water bath 5 and the temperature probe 6.

In the following embodiments, various processes and methods that are not described in detail are conventional methods known in the art. The source, trade name and components of the reagents used are indicated at the first appearance, and the same reagents used thereafter are the same as those indicated for the first time unless otherwise specified.

Example 1

Curculio vulgaris 10 parts, Xianling spleen 10 parts, Eucommia ulmoides 10 parts, Morinda officinalis 10 parts,

Astragalus 10 parts, Angelica 10 parts, Achyranthes bidentata 10 parts, Codonopsis 10 parts, Fried Baizhu 5 parts,

Poria 5 parts, Guizhi 5 parts, Fried white peony 5 parts, Cuscuta 5 parts, Antler slices 10 parts,

20 parts of Huai wheat, 5 parts of licorice, 5 parts of tangerine peel, 10 parts of Chinese wolfberry, 10 parts of Rehmannia glutinosa.

Eucommia ulmoides are fried eucommia ulmoides, sprinkle the raw eucommia ulmoides with salt water and mix well, wait until it is exhausted, and then stir-fry the eucommia ulmoides with a slow fire until it is yellow-brown and used as medicine (the same below).

Astragalus is honey-dried astragalus, that is, astragalus slices are mixed well with honey, and when they are not sticky, they are taken out and aired, and then used as medicine (the same below). Licorice is Zhigancao (the same below).

(1) The above-mentioned raw materials are pulverized in parts by mass, passed through an 80-mesh sieve, and then thoroughly mixed.

(2) Add tap water at 1:10 (m/v), and use a multi-mode ultrasonic extractor for extraction at a temperature of 60°C. The specific parameters of multi-mode ultrasonic extraction are ultrasonic time 10min, ultrasonic power 60W/L, and ultrasonic frequency 35kHz/60kHz. After the extraction, the supernatant was poured and the same amount of tap water was added, and the extraction process was repeated for 1 round. The combined extracts are filtered to remove impurities, and the filtrate is vacuum concentrated and freeze-dried to prepare a traditional Chinese medicine composition.

Example 2

Curculigo curcuma 20 parts, Ziheche 20 parts, Eucommia ulmoides 20 parts, Morinda officinalis 20 parts, Astragalus 20 parts,

Angelica 20, Achyranthes 20, Codonopsis 20, Fried Atractylodes 15, Poria, 15

Guizhi 10 parts, fried white peony 15 parts, dodder 15 parts, deer horn slices 20 parts, Huai wheat 40 parts,

15 parts of licorice, 15 parts of tangerine peel, 20 parts of wolfberry, 20 parts of Rehmannia glutinosa.

(1) After crushing the above raw materials in parts by mass and passing through a 100-mesh sieve,

(2) Each raw material is added to mineral water at a ratio of 1:20 (m/v), and the multi-mode ultrasonic extractor is used for extraction at a temperature of 70°C. The specific parameters of the multi-mode ultrasonic extraction are ultrasonic time 40min and ultrasonic power 180W/ L, the ultrasonic frequency is 60kHz. After the extraction, pour the supernatant and add the same amount of tap water or mineral water, and repeat the extraction process for 1 round; the combined extracts are filtered to remove impurities, and the filtrate is vacuum concentrated to obtain a single Chinese medicine extract. After crushing and passing through an 80-mesh sieve, it is fully mixed with a high-speed mixer, the mixing time is 20 min, and the speed of the high-speed mixer is 60 r/min. After thorough mixing, the formula powder product of this embodiment is prepared.

Example 3

A traditional Chinese medicine composition for improving male sexual function and anti-fatigue, prepared from the following raw materials in parts by weight:

15 copies of Curculigo, 15 copies of Xianling Spleen, 15 copies of Eucommia ulmoides, 15 copies of Morinda officinalis,

Astragalus 15 parts, Angelica 15 parts, Achyranthes bidentata 15 parts, Codonopsis 15 parts,

Stir-fried Atractylodes macrocephala 9 servings, Poria cocos 12 servings, Guizhi 6 servings, Stir-fried white peony root 12 servings,

Cuscuta 12 parts, Antler slice 15 parts, Huai wheat 30 parts, Licorice 6 parts,

6 parts of tangerine peel, 15 parts of Chinese wolfberry, 15 parts of Rehmannia glutinosa.

(2) Add mineral water according to 1:10 (m/v), and use a multi-mode ultrasonic extractor for extraction at a temperature of 60°C. The specific parameters of multi-mode ultrasonic extraction are the ultrasonic time is 30min; the ultrasonic power is 150W/L; the ultrasonic frequency is 20kHz/35kHz/60kHz. After the extraction, the supernatant was poured and the same amount of mineral water was added, and the extraction process was repeated for 1 round. The combined extracts are filtered to remove impurities, and the filtrate is vacuum concentrated and freeze-dried to prepare a traditional Chinese medicine composition.

Example 4

Curculigo curcuma 20 parts, Cynomorium 20 parts, Eucommia ulmoides 20 parts, Morinda officinalis 20 parts, Astragalus 20 parts,

Angelica 20, Achyranthes 20, Codonopsis 20, Fried Atractylodes 15, Poria, 15

Guizhi 10 parts, fried white peony 15 parts, dodder 15 parts, tortoise shell slices 20 parts, Huai wheat 40 parts,

(2) Add 60% ethanol-water solution at a ratio of 1:20 (m/v) for each raw material, and use a multi-mode ultrasonic extractor for extraction at a temperature of 70°C. The specific parameters of the multi-mode ultrasonic extraction are the ultrasonic time is 30min; the ultrasonic power is 150W/L; the ultrasonic frequency is 35kHz/60kHz. After the extraction, the supernatant was poured and the same amount of 60% ethanol-water solution was added. Repeat the extraction process for 1 round; the combined extracts were filtered to remove impurities, and the filtrate was concentrated in vacuo to obtain a single Chinese medicine extract. After drying, pulverizing, and passing through an 80-mesh sieve, it is fully mixed with a high-speed mixer, the mixing time is 20 minutes, and the speed of the high-speed mixer is 60 r/min. After thorough mixing, the formula powder product of this embodiment is prepared.

Example 5

Xianling spleen 10 parts, Eucommia ulmoides 10 parts, Morinda officinalis 10 parts, Astragalus 10 parts, Angelica sinensis 10 parts,

Achyranthes bidentata 10 parts, Codonopsis 10 parts, Fried Atractylodes 5 parts, Poria cocos 5 parts, Fried white peony 5 parts,

Cuscuta seed 5 parts, Huai wheat 20 parts, Licorice 5 parts, Tangerine peel 5 parts, Chinese wolfberry 10 parts,

Rehmannia glutinosa 10 copies.

The above-mentioned Chinese medicines belong to the list of Chinese medicines that can be used as health foods published by the National Health and Family Planning Commission or the list of Chinese medicines that are both food and medicine published by the National Health and Family Planning Commission.

(2) Add tap water or mineral water at 1:15 (m/v), and use a multi-mode ultrasonic extractor for extraction at a temperature of 60°C. The specific parameters of multi-mode ultrasonic extraction are the ultrasonic time is 20min; the ultrasonic power is 100W/L; the ultrasonic frequency is 35kHz/40kHz. After the extraction is finished, pour the supernatant and add the same amount of tap water or mineral water, and repeat the extraction process for 1 round. The combined extracts are filtered to remove impurities, and the filtrate is vacuum concentrated and freeze-dried to prepare a traditional Chinese medicine composition.

Example 6

Xianling spleen 20 parts, Eucommia ulmoides 20 parts, Morinda officinalis 20 parts, Astragalus 20 parts, Angelica sinensis 20 parts,

Achyranthes bidentata 20 parts, Codonopsis 20 parts, Fried Atractylodes 15 parts, Poria cocos 15 parts, Fried white peony 15 parts,

Dodder 15 parts, Huai wheat 40 parts, Licorice 15 parts, Tangerine peel 15 parts, Chinese wolfberry 20 parts,

Rehmannia glutinosa 20 copies.

(2) Each raw material is added to tap water or mineral water at a ratio of 1:20 (m/v), and the multi-mode ultrasonic extractor is used for extraction at a temperature of 60°C. The specific parameters of multi-mode ultrasonic extraction are the ultrasonic time is 40min; the ultrasonic power is 180W/L; the ultrasonic frequency is 28kHz/60kHz. After the extraction, pour the supernatant and add the same amount of tap water or mineral water, and repeat the extraction process for 1 round; the combined extracts are filtered to remove impurities, and the filtrate is vacuum concentrated to obtain a single Chinese medicine extract. After crushing and passing through an 80-mesh sieve, it is fully mixed with a high-speed mixer, the mixing time is 20 min, and the speed of the high-speed mixer is 60 r/min. After thorough mixing, the formula powder product of this embodiment is prepared.

Example 7

Xianling spleen 15 parts, Eucommia ulmoides 15 parts, Morinda officinalis 15 parts, Astragalus 15 parts, Angelica 15 parts, Achyranthes bidentata 15 parts, Codonopsis pilosula 15 parts, Fried atractylodes 9 parts, Poria cocos 12 parts, Fried white peony 12 parts,

Cuscuta 12 parts, Huai wheat 30 parts, Licorice 6 parts, Tangerine peel 6 parts, Chinese wolfberry 15 parts,

Rehmannia glutinosa 15 copies.

(2) Add mineral water according to 1:15 (m/v), and use a multi-mode ultrasonic extractor for extraction at a temperature of 60°C. The specific parameters of multi-mode ultrasonic extraction are the ultrasonic time is 40min; the ultrasonic power is 160W/L; the ultrasonic frequency is 28kHz/60kHz. After the extraction is finished, pour the supernatant and add the same amount of tap water or mineral water, and repeat the extraction process for 1 round. The combined extracts are filtered to remove impurities, and the filtrate is vacuum concentrated and freeze-dried to prepare a traditional Chinese medicine composition.

Comparative example: conventional extraction steps: the raw materials in the above different embodiments were respectively crushed according to mass parts, passed through an 80-mesh sieve, and mixed thoroughly. Add tap water at 1:10 (m/v), and perform extraction at 60°C and 100°C. After the extraction, the supernatant was poured and the same amount of tap water was added, and the extraction process was repeated for 1 round. The combined extracts are filtered to remove impurities, and the filtrate is vacuum concentrated and freeze-dried to prepare a traditional Chinese medicine composition.

The method for measuring the total extraction rate of the effective ingredients in the Chinese medicine compound of the present invention is as follows:

Pour the supernatant of the extract obtained after extraction into dry and weighed filter paper and a glass funnel, filter into an Erlenmeyer flask, wash the beaker and filter paper twice with a small amount of ethanol, and then place the residue and filter paper in the glass funnel Dry to constant weight at 50°C. The total extraction rate is calculated according to the following formula:

Where: m—mass of sample (g); m ₁ —mass of filter paper and funnel (g); m ₂ —mass of filter paper, funnel and residue (g).

Table 1 Comparison between the ultrasonic extraction method of the present invention and the conventional method

The functional activity evaluation methods used in the specific embodiments of the present invention are as follows:

(1) To improve the test of sexual function, refer to the method of Bao Lei et al. for specific operation steps (Experimental study on the effect of Jilin ginseng oligopeptide on the sexual function of male mice, Bao Lei et al., Chinese Journal of Preventive Medicine, October 2015, Vol. 16 Issue 10).

(2) The animals used in the evaluation test were healthy male ICR mice, aged 5 months, weighing 18-22g, provided by the Animal Breeding Center of Jiangsu University. The animals were bred in the barrier system of the Experimental Animal Center of Jiangsu University, and the breeding environment was half day and night. Free drinking and drinking, the temperature of eating is maintained at 24±2℃, the relative humidity is 50% to 60%, and the indoor lighting is controlled at a 12h/12h light-dark cycle rhythm.

The results of the functional activity evaluation test using the formulas of the various embodiments of the present invention show that after the intervention of the formulas of the various embodiments of the present invention, the mating latency of mice is shortened, and the number of mating times within 20 minutes is significantly increased. It can be seen that the formula of the present invention is one This kind of good function of enhancing sexual function can improve the libido and sexual activity ability of mice to a certain extent. At the same time, the number of sperm in the mice increased. Compared with the control group, the sperm aberration rate of the treatment groups of each example decreased, and the sperm viability rate increased. It shows that the formula of the present invention can effectively inhibit sperm aberrations, improve sperm motility, and can promote sperm counts in mice to varying degrees (Table 2).

The results of the functional activity evaluation test using the formulas of the various embodiments of the present invention show that the weight of the accessory organs of male mice and the nitric oxide (NO) in the serum of male mice can be significantly increased after intragastric administration of the formulas of the various embodiments of the present invention. And testosterone concentration, NO concentration can enhance penile erection ability, testosterone concentration can promote the production of nitric oxide synthase (NOS), inhibit the production of phosphodiesterase type 5 (PDE5), therefore, the formula of the present invention has a significant improvement Sexual function (Table 3).

Table 2 The effect of the embodiment of the present invention on the incubation period, mating frequency and sperm quality of mice

Table 3 The effect of the embodiment of the present invention on the organ index of mouse sexual organs and the content of NO and testosterone in serum

Claims

A Chinese medicinal composition for improving male sexual function and anti-fatigue, which is characterized by being prepared from the following parts by weight of Chinese medicinal materials:
The Chinese medicinal composition for improving male sexual function and anti-fatigue according to claim 1, characterized in that the Xianlingpi (10-20 parts) in the Chinese medicinal materials can also be replaced with Cynomorium (10-20 parts);

In the above-mentioned Chinese medicinal materials, Xianling Spleen (10-20 parts) or Ziheche (10-20 parts) may be used instead, and Lujiao tablets (10-20 parts) shall be replaced by tortoise shell slices (10-20 parts);

The eucommia ulmoides described is fried eucommia ulmoides for raw eucommia ulmoides, sprayed with salt water and mixed well, wait for exhaustion, and then stir-fried with a slow fire until it is yellowish brown and used as medicine;

The said astragalus is honey-dried astragalus, that is, the astragalus slices are mixed with honey, and when they are not sticky, they are taken out and aired, and then used as medicine;

The licorice described therein is lucid licorice.
The traditional Chinese medicine composition for improving male sexual function and anti-fatigue according to claim 1, characterized in that it is prepared from the following raw materials in parts by weight:
A Chinese medicinal composition for improving male sexual function and anti-fatigue according to claim 3, characterized in that the Xianlingpi (15 parts) of the Chinese medicinal materials can also be replaced by Cynomorium (15 parts);

In the above-mentioned Chinese medicinal materials, Xianlingpi (15 parts) or Ziheche (15 parts) can be used instead, and Lujiao tablets (15 parts) can be replaced by tortoise shell tablets (15 parts).
The preparation method of a traditional Chinese medicine composition for improving male sexual function and anti-fatigue according to claims 1-4, characterized in that it is carried out according to the following steps:

(1) Crush the above-mentioned raw materials in parts by mass, pass through a sieve of 80-100 mesh, and mix them thoroughly;

(2) Add tap water or mineral water according to 1:10～20 (m/v), and use a multi-mode ultrasonic extractor for extraction at a temperature of 60～70℃; after extraction, pour the supernatant and add the same amount Repeat this extraction process for 1 round of tap water or mineral water; the combined extracts are filtered to remove impurities, and the filtrate is vacuum concentrated and freeze-dried to obtain a Chinese medicine composition.
A method for preparing a traditional Chinese medicine composition for improving male sexual function and anti-fatigue according to claim 5, wherein the extraction solvent in step (2) can also be used in volume fraction in addition to tap water or mineral water 40-70% ethanol-water solution;

The specific parameters of the multi-mode ultrasonic extraction in step (2) are ultrasonic time 10min-40min, preferably ultrasonic time 30min; ultrasonic power 60W/L-180W/L, preferably ultrasonic power 150W; ultrasonic frequency 20kHz, 28kHz, 35kHz , 40kHz, 60kHz, or a combination of two or two, or a combination of three or three, preferably the ultrasonic frequency 35kHz/60kHz or 20kHz/35kHz/60kHz.
The preparation method of a traditional Chinese medicine composition for improving male sexual function and anti-fatigue according to claims 1-4, which is characterized in or carried out according to the following steps:

(1) After pulverizing the above raw materials in parts by mass and passing through a sieve of 80-100 mesh,

(2) Add tap water or mineral water at a ratio of 1:10-20 (m/v) for each raw material, and use a multi-mode ultrasonic extractor for extraction at a temperature of 60-70°C; after the extraction is complete, pour the supernatant and Add the same amount of tap water or mineral water, repeat the extraction process for 1 round; combine the extracts to filter and remove impurities, the filtrate is vacuum concentrated, spray-dried or freeze-dried to obtain a single Chinese medicine extract, and then mix according to the above-mentioned raw material ratio The traditional Chinese medicine composition can be obtained;

Wherein, in addition to tap water or mineral water, the extraction solvent described in step (2) can also use ethanol-water solution with a volume fraction of 40-70%;

The specific parameters of the multi-mode ultrasonic extraction in step (2) are ultrasonic time 10min-40min, preferably ultrasonic time 30min; ultrasonic power 60W/L-180W/L, preferably ultrasonic power 150W; ultrasonic frequency 20kHz, 28kHz, 35kHz , 40kHz, 60kHz, or a combination of two or two, or a combination of three or three, preferably the ultrasonic frequency 35kHz/60kHz or 20kHz/35kHz/60kHz.
A health food for improving male sexual function and anti-fatigue, which is characterized by being prepared from the following parts by weight of Chinese medicinal materials:
The health food for improving male sexual function and anti-fatigue according to claim 8, characterized in that it is prepared from the following parts by weight of raw materials:
The method for preparing a health food for improving male sexual function and anti-fatigue according to claim 8 or 9, characterized in that it is carried out according to the following steps:

(1) Crush the above-mentioned raw materials in parts by mass, pass through a sieve of 80-100 mesh, and mix them thoroughly;

(2) Add tap water or mineral water according to 1:10～20 (m/v), and use a multi-mode ultrasonic extractor for extraction at a temperature of 60～70℃; after extraction, pour the supernatant and add the same amount Repeat the extraction process for 1 round of tap water or mineral water; combine the extracts to filter and remove impurities, and the filtrate is vacuum concentrated, spray-dried or freeze-dried to prepare a traditional Chinese medicine composition;

Wherein, in addition to tap water or mineral water, the extraction solvent described in step (2) can also use ethanol-water solution with a volume fraction of 40-70%;

The specific parameters of the multi-mode ultrasonic extraction in step (2) are ultrasonic time 10min-40min, preferably ultrasonic time 30min; ultrasonic power 60W/L-180W/L, preferably ultrasonic power 150W; ultrasonic frequency 20kHz, 28kHz, 35kHz , 40kHz, 60kHz, or a combination of two or two, or a combination of three or three, preferably the ultrasonic frequency 35kHz/60kHz or 20kHz/35kHz/60kHz;

Or follow the steps below:

(1) After pulverizing the above raw materials in parts by mass and passing through a sieve of 80-100 mesh,

(2) Add tap water or mineral water at a ratio of 1:10-20 (m/v) for each raw material, and use a multi-mode ultrasonic extractor for extraction at a temperature of 60-70°C; after the extraction is complete, pour the supernatant and Add the same amount of tap water or mineral water, repeat the extraction process for 1 round; combine the extracts to filter and remove impurities, the filtrate is vacuum concentrated, spray-dried or freeze-dried to obtain a single Chinese medicine extract, and then mix according to the above-mentioned raw material ratio The traditional Chinese medicine composition can be obtained;

Wherein, in addition to tap water or mineral water, the extraction solvent described in step (2) can also use ethanol-water solution with a volume fraction of 40-70%;

The specific parameters of the multi-mode ultrasonic extraction in step (2) are ultrasonic time 10min-40min, preferably ultrasonic time 30min; ultrasonic power 60W/L-180W/L, preferably ultrasonic power 150W; ultrasonic frequency 20kHz, 28kHz, 35kHz , 40kHz, 60kHz, or a combination of two or two, or a combination of three or three, preferably the ultrasonic frequency 35kHz/60kHz or 20kHz/35kHz/60kHz.