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  • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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  • C12N2750/14011—Parvoviridae
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  • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
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  • C12N2750/14011—Parvoviridae
  • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
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  • C12N2810/00—Vectors comprising a targeting moiety
  • C12N2810/40—Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source

Definitions

• the present invention relates to adeno-associated virus capsid polypeptide sequences and their use in therapeutic transgene delivery to the eye targeting the photoreceptors or retinal pigment epithelial cells.
• PRs retinal photoreceptor cells
• ROS retinal pigment epithelial
• ALD age-related macular degeneration
• PRs retinal pigment epithelial
• RP retinal pigment epithelial
• ALD age-related macular degeneration
• the genetic mutations responsible for many forms of photoreceptor degeneration are identified, enabling interventions by administration of therapeutic transgenes.
• Existing PR targeting nucleic acid-based therapies are effective only at the early disease stages when damage to PR is minimal.
• Gene therapy employing a suitable optogenetic protein that confers light sensitivity to functional inner retinal cells might potentially restore vision even in later disease stages. To make such approaches applicable in a clinical setting, improved vectors with better retinal penetration for gene delivery are required.
• Recombinant vectors based on adeno-associated virus are candidates for therapeutic gene transfer in the eye.
• AAV vectors are typically applied subretinally.
• Subretinal application has been shown to lead to a decrease in retinal thickness and visual acuity.
• subretinal application only achieves gene transfer and expression in cells immediately adjacent to the bulk of injected fluid, while an effective administration method should reach cells along the entire width of the retina.
• Intravitreal injection into the jelly-like filling of the eye does not only have the potential to deliver the vector to the entire retina, but is also much safer and far less technically demanding than subretinal injection.
• the inner limiting membrane (ILM) that separates the neural retina from the vitreous humor is abundant in native receptors of many AAV serotypes and therefore presents a daunting barrier for AAV vectors.
• Engineered AAV vectors that penetrate better through the ILM and other retinal barriers when delivered intravitreally are needed to increase efficacy and safety of ocular AAV gene therapy.
• the objective of the present invention is to provide means and methods for gene transfer into the retina enabling targeting of all retinal cell types, particularly inner retinal cells and photoreceptors. This objective is attained by the subject-matter of the independent claims of the present specification. Summary of the Invention
• a first aspect of the invention relates to an adeno-associated virus (AAV) capsid polypeptide comprising a peptide insertion at position 453 or 587/588 of the AAV serotype 2 capsid or a position homologous thereto in an AAV capsid of another serotype (Table 3: #1, #2), located at the highest and second highest capsid protrusions, respectively.
• AAV adeno-associated virus
• SASEAST Cap3; SEQ ID NO 10
• DTRPHDQ Cap5; SEQ ID NO 11
• EHYNSTC Cap7; SEQ ID NO 12
• PNPNCTL Cap9; SEQ ID NO 13
• TPPSITA Cap11; SEQ ID NO 14
• CGESSYL Cap12; SEQ ID NO 15
• PRTPHTA Cap13; SEQ ID NO 16
• ELCDGFA Cap14; SEQ ID NO 17
• the peptide may be flanked by short stretches of other amino acids (1, 2, 3, 4, 5 or even 6 AA).
• flanking amino acids to embed the indicated sequence at the indicated position within the capsid sequence can be selected from (but are not limited to) Ala, Leu, Gly, Ser, and Thr.
• This peptide insert increases the infection efficacy of an adeno-associated virus and/or transduction efficacy of an adeno-associated vector displaying the peptide insert on the capsid surface at I-587 above indicated position at least for the tested cell types and tissues.
• a second aspect of the invention relates to a nucleic acid sequence encoding the AAV capsid polypeptide according to the first aspect.
• Particular embodiments include the inclusion of this nucleic acid sequence in an AAV cap sequence, particularly in an AAV serotype 2 capsid sequence for generation of a capsid-engineered AAV vector.
• a third aspect of the invention relates to an agent selected from the AAV capsid polypeptide, an AAV vector, and the nucleic acid sequence for treatment of a condition affecting the retina or an RPE cell.
• Alternative forms of this aspect are embodied by methods of treatment of conditions affecting photoreceptors or RPE cells, comprising the administration of the agent according to the invention to a patient in need thereof.
• Administration forms comprising the agents of the invention are further aspects of the invention.
• Fig. 1 Retinal layer specificity of expression for selected capsid variants.
• Fig. 2 Examples of pan-retinal transgene expression throughout the outer murine retina after intravitreal injection.
• AAV2(M6) and AAV2(7m8) after intravitreal injection into the mouse eye after intravitreal injection into the mouse eye.
• AAV in the context of the present specification relates to adeno-associated virus.
• AAV vector in the context of the present specification relate to a viral vector composed of 60 AAV capsid proteins and an encapsidated AAV nucleic acid.
• An AAV vector is derived from an AAV virion, but the AAV vector is engineered to be replication-incompetent in the presence of a helper virus by removing the rep and cap genes from the AAV genome.
• the encapsidated AAV nucleic acid may comprise a transgene which is to be delivered into a target cell.
• AAV capsid in the context of the present specification relates to a polypeptide encoded by engineered capsid (cap) genes generated by the inventors and listed herein below.
• the AAV capsid disclosed herein may be used to assemble recombinant adeno-associated viral vectors for gene therapy.
• Reference to an amino acid position in the AAV capsid in the context of the present specification relates to the capsid amino acid sequence of adeno-associated virus 2 capsid protein VP1 having the sequence of SEQ ID NO. 1 (Table 2) (GenBank accession number of the corresponding nucleic acid sequence is J01901.1). The corresponding amino acid positions for other homologous adeno-associated virus serotypes are shown in Table 3.
• homologous in the context of the present specification relates to nucleic acid or amino acid sequences derived from different serotypes of AAV. The corresponding positions of different adeno-associated virus serotypes are shown in Table 3.
• transgene in the context of the present specification relates to a gene or genetic material that has been transferred from one organism to another. In the present context, the term may also refer to transfer of the natural or physiologically intact variant of a genetic sequence into tissue of a patient where it is missing. It may further refer to transfer of a natural encoded sequence the expression of which is driven by a promoter absent or silenced in the targeted tissue.
• a recombinant in the context of the present specification relates to a nucleic acid, which is the product of one or several steps of cloning, restriction and/or ligation and which is different from the naturally occurring nucleic acid.
• a recombinant virus particle comprises a recombinant nucleic acid.
• intravitreal administration in the context of the present specification relates to a route of administration of a pharmaceutical agent, for example a viral vector, in which the agent is delivered into the vitreous body of the eye.
• Intravitreal administration is a procedure to place a medication directly into the space in the back of the eye called the vitreous cavity, which is filled with a jelly-like fluid called the vitreous humour gel.
• subretinal administration in the context of the present specification relates to a route of administration of a pharmaceutical agent, particularly a viral vector in the context of this specification, into the space between retinal pigment epithelium (RPE) cells and photoreceptors.
• a pharmaceutical agent particularly a viral vector in the context of this specification
• polypeptide in the context of the present specification relates to a molecule consisting of 50 or more amino acids that form a linear chain wherein the amino acids are connected by peptide bonds.
• the amino acid sequence of a polypeptide may represent the amino acid sequence of a whole (as found physiologically) protein or fragments thereof.
• polypeptides and protein are used interchangeably herein and include proteins and fragments thereof. Polypeptides are disclosed herein as amino acid residue sequences.
• Amino acid residue sequences are given from amino to carboxyl terminus.
• Capital letters for sequence positions refer to L-amino acids in the one-letter code (Stryer, Biochemistry, 3 rd ed. p. 21).
• Lower case letters for amino acid sequence positions refer to the corresponding D- or (2R)-amino acids. Sequences are written left to right in the direction from the amino to the carboxy terminus.
• amino acid residue sequences are denominated by either a three letter or a single letter code as indicated as follows: Alanine (Ala, A), Arginine (Arg, R), Asparagine (Asn, N), Aspartic Acid (Asp, D), Cysteine (Cys, C), Glutamine (Gin, Q), Glutamic Acid (Glu, E), Glycine (Gly, G), Histidine (His, H), Isoleucine (lie, I), Leucine (Leu, L), Lysine (Lys, K), Methionine (Met, M), Phenylalanine (Phe, F), Proline (Pro, P), Serine (Ser, S), Threonine (Thr, T), Tryptophan (Trp, W), Tyrosine (Tyr, Y), and Valine (Val, V).
• variant refers to a polypeptide that differs from a reference polypeptide, but retains essential properties.
• a typical variant of a polypeptide differs in its primary amino acid sequence from another polypeptide used as reference. Generally, differences are limited so that the sequences of the reference polypeptide and the variant are closely similar overall and, in many regions, identical.
• a variant and reference polypeptide may differ in amino acid sequence by one or more modifications (e.g., substitutions, additions, and/or deletions).
• a variant of a polypeptide may be naturally occurring such as an allelic variant, or it may be a variant that is not known to occur naturally.
• biological activity in the context of the present specification relates to a specifically measurable quantity displayed by certain variants of the polypeptides enclosed herein.
• a viral vector e.g. firefly luciferase
• the biological activity of a capsid variant may be assayed by measuring expression of a transgene (e.g. firefly luciferase) into cultured cells or into a model organism in-vivo in a standard assay.
• a transgene e.g. firefly luciferase
• sequence identity and percentage of sequence identity refer to the values determined by comparing two aligned sequences.
• Methods for alignment of sequences for comparison are well-known in the art. Alignment of sequences for comparison may be conducted by the local homology algorithm of Smith and Waterman, Adv. Appl. Math. 2:482 (1981), by the global alignment algorithm of Needleman and Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson and Lipman, Proc. Nat. Acad. Sci. 85:2444 (1988) or by computerized implementations of these algorithms, including, but not limited to: CLUSTAL, GAP, BESTFIT, BLAST, FASTA and TFASTA. Software for performing BLAST analyses is publicly available, e.g., through the National Center for Biotechnology-Information (http://blast.ncbi.nlm.nih.gov/).
• sequence identity values refer to the value obtained using the BLAST suite of programs (Altschul et al., J. Mol. Biol. 215:403-410 (1990)) using the above identified default parameters for protein and nucleic acid comparison, respectively.
• amino acid linker refers to an oligopeptide of variable length that is used to connect two polypeptides in order to generate a single chain polypeptide.
• exemplary embodiments of linkers useful for practicing the invention specified herein are oligopeptide chains consisting of 1 to 6 amino acids.
• a non-limiting example of an amino acid linker is AAA or AA, as exemplified below.
• AAV is a small non-pathogenic virus that infects humans and other primate species.
• the AAV2 infection starts by docking to the cell surface receptor heparan sulphate proteoglycan (HSPG). Its low-affinity binding to glycans induces a reversible structural rearrangement of the capsid that promotes binding to the co-receptor crv/35 or cr5/31 integrin inducing formation of a clathrin-coated pit.
• the clathrin-coated pit becomes internalized via endocytosis and the viral particles are transported to the nucleus.
• the pH drops due to acidification of the endosomal compartments, which is a feature of the endosomal vesicle maturation. Acidification-triggered conformational change takes place in the capsid, and the virus escapes from the late endosome by lipolytic pore formation.
• the genome is built of a 4.7 kilobase long single stranded DNA (ssDNA), either positive- or negative-sensed.
• the genome comprises three open reading frames (ORFs) flanked by inverted terminal repeats (ITRs).
• ITRs are self-complementary, CG-rich, T- shaped hairpins at the 5’ and 3’-end of the AAV genome and the only necessary viral component present in recombinant vector genomes.
• the ITR include a terminal resolution site (TRS) and a Rep binding element (RBE), which facilitate replication and encapsidation of the viral genome.
• the ORFs encode the genes rep, cap, AAP.
• Cap encodes the capsid proteins VP1, VP2 and VP3, which interact together to form a capsid of an icosahedral symmetry, and the assembly-activating protein (AAP), which is required for stabilizing and transporting newly produced VP proteins from the cytoplasm into the cell nucleus.
• All three VPs are translated from one mRNA and spliced differently. The largest 90 kDa VP1 is an unspliced transcript, the 72 kDa VP2 is translated from a non-conventional ACG start codon whereas the smallest 60 kDa VP3 is translated from an AUG codon. All the three VPs have overlapping C-termini.
• the VP3 constitutes 90% of the capsid and VP1 and VP2 share a common C-terminal amino acid sequence with VP3 but have N-terminal extensions that stay buried inside the capsid.
• the VP1 unique N-terminal sequence contains phospholipase A2 (PLA2) activity that is required for infection, and nuclear localization signals.
• PHA2 phospholipase A2
• a first aspect of the invention relates to an adeno-associated virus (AAV) capsid polypeptide comprising a peptide insert at a peak or a spiky protrusion at position 453 or 587 of the AAV serotype 2 capsid.
• AAV adeno-associated virus
• SASEAST Cap3; SEQ ID NO 10
• DTRPHDQ Cap5; SEQ ID NO 11
• EHYNSTC Cap7; SEQ ID NO 12
• PNPNCTL Cap9; SEQ ID NO 13
• TPPSITA Cap11 ; SEQ ID NO 14
• CGESSYL Cap12; SEQ ID NO 15
• PRTPHTA Cap13; SEQ ID NO 16
• ELCDGFA Cap14; SEQ ID NO 17
• the insert is selected from SASEAST (Cap3; SEQ ID NO 10), TPPSITA (Cap11; SEQ ID NO 14), PRTPHTA (Cap13; SEQ ID NO 16) and ELCDGFA (Cap14; SEQ ID NO 17).
• the peptide insert as laid out above may be comprised in a 7-13mer consisting of one of the above sequence, flanked by 0-6 linker amino acids (6 being the maximum number of the total of N and C terminally flanking amino acids) selected from, but not limited to, Ala, Leu, Gly, Ser, and Thr inserted N- and/or C-terminally of the insert sequences given in the preceding paragraph.
• AAV2 sequences given herein are in relation to the reference sequence accessible at GenBank entry No. J01901.1 (Adeno-associated virus 2, complete genome).
• GenBank entry No. J01901.1 Addeno-associated virus 2, complete genome.
• the corresponding amino acid sequence is given as SEQ ID NO 1 in table 2.
• Spiky protrusions represent the most exposed regions of the capsids. The highest peak is located at amino acid position 453 and second highest at position 587 on the AAV2 capsid. These peaks accept peptide insertions without disturbing capsid assembly and provide opportunities for targeting non-permissive cells. Likewise, protrusions represent critical sites of AAVs host interaction, receptor binding and immunogenicity. Of note, in certain embodiments, R585 and 588 are mutated to attain optimal efficiency if the insert is inserted at position 453.
• the peptide insert may be directly after (in C-terminal direction) this position or one to six amino acids further in C-terminal direction.
• the peptide insert defined to be at position 587 an insertion defined as between the pre insertion positions 587 and 588, may start at position 588 or 589 (one or two amino acids further in C-terminal direction) or 590 (three amino acids further in C-terminal direction). All of these insert positions allow for peptide inserts that do not disturb the overall capsid structure, but rather may increase transduction efficacy.
• This peptide insert increases the retinal penetration and transduction efficacy of the virus after intravitreal delivery.
• the peptide insert is selected from SASEAST (Cap3; SEQ ID NO 10), DTRPHDQ (Cap5; SEQ ID NO 11), EHYNSTC (Cap7; SEQ ID NO 12), PNPNCTL (Cap9; SEQ ID NO 13), TPPSITA (Cap11; SEQ ID NO 14), CGESSYL (Cap12; SEQ ID NO 15), PRTPHTA (Cap13; SEQ ID NO 16) and ELCDGFA (Cap14; SEQ ID NO 17), In certain embodiments, the peptide insert is selected from SASEAST (Cap3; SEQ ID NO 10), TPPSITA (Cap11; SEQ ID NO 14), PRTPHTA (Cap13; SEQ ID NO 16) and ELCDGFA (Cap14; SEQ ID NO 17).
• the peptide insert can be present in the AAV capsid polypeptide as is or in the context of 1,
• Suitable spacer AAs include, but are not limited to alanine, leucine, glycine, threonine and serine.
• the insertion between Ns87 and R S88 of the AAV2 VP1 is a 12mer of the form RsssG N A A A A Xi X 2 X 3 X 4 X 5 X 6 X / -A A Rsss Q A A, whereas X1-X7 represents the inserted heptamer oligo and A the flanking linkers. It is to be understood that this is an example only and the invention is not limited to inserts exactly at this position having exactly these flanking sequences.
• the oligo is always inserted between positions 587 and 588 on VP1 of AAV2. It is an insertion, so the VP1 AAV2 numbering does not change and subsequent positions are counted “without the insert” with respect to substitutions and the like.
• the inserted peptide contains the heptamer as specified herein, but may potentially also be shorter to function or longer.
• the inventors inserted a 12-mer in the form of AAA-X1X2X3X4X5X7-AA, whereas the linker amino acids can be freely selected from Ala, Leu, Gly, Ser, Thr.
• the N- and C-terminal linkers can be of 0-5 AA length.
• an insertion peptide can be of 5-13 amino acids length, comprising 0-6 linker amino acids.
• the peptide insertion (elsewhere in this specification referred to as “oligo”) can move 1-5AA further to the C-terminus.
• the insertion site can also be at (e.g., immediately C-terminal to) amino acid 453.
• any of the peptide sequences recited above are inserted at position 453 of SEQ ID NO 001 instead of position 587.
• the position of insertion may vary and the peptide sequence may be flanked by inserts as discussed above and may consist of 5-13 AAs.
• R587A and R588A mutations may be introduced into VP1 of AAV2 (Boucas Jet al. J Gene Med. 2009 Dec;11(12):1103-13. doi: 10.1002/jgm.1392)
• a position homologous to position 587/588 or 453 in AAV serotype 2 can be selected (Table 3: #1, #2) to construct an AAV of another serotype with one of the inserted peptide sequences recited above.
• the AAV capsid protein is characterized by one or several tyrosine to phenylalanine substitutions of tyrosine residues, wherein the tyrosine residues occur in the wild-type capsid sequence at positions 252, 272, 444, 500, 700, 704 and 730.
• capsid variants herein were selected by evolutionary methods in the context of a tyrosine-modified capsid.
• the AAV capsid protein is characterized by one or several tyrosine to phenylalanine substitutions at positions 252, 272, 444, 500, 704 and 730.
• the AAV capsid protein is characterized by several tyrosine to phenylalanine substitutions at all of the positions 252, 272, 444, 500, 700 and 730.
• a tyrosine position homologous to any of the positions 252, 272, 444, 500, 700, 704 and 730 in AAV serotype 2 can be selected for substitution to phenylalanine (Table 3: #3 - #9) to construct an AAV of another serotype with tyrosine to phenylalanine substitutions. Also, other tyrosine positions may be substituted for phenylalanine.
• the AAV capsid protein is characterized by one or several threonine to valine substitutions, particularly T491V.
• a threonine position homologous to the position 491 in AAV serotype 2 can be selected for substitution to valine (Table 3: #10) to construct an AAV of another serotype with a threonine to valine substitution.
• the tyrosine to phenylalanine substitutions mentioned above are combined with the threonine to valine substitutions, particularly T491 V.
• Y-F and T-V mutations decrease ubiquitination and thus proteasomal degradation of the viral particle once internalized by the cell, thereby increasing transduction efficacy.
• the adeno-associated virus capsid polypeptide comprises the amino acid sequence selected from SEQ ID NO 2 - SEQ ID NO 9, or a sequence having at least 85% identity thereto and at least 90% of the biological activity of a sequence selected from SEQ ID NO 2 - SEQ ID NO 9.
• this biological activity can be measured as follows: HEK293 cells are co-transfected with a plasmid encoding the test or the reference polypeptide, the plasmid harbouring helper adenoviral genes and a transgene plasmid, selected from scCMV-mCitrine, scEfla-mCitrine, scCMV-EGFP, scEf1a-EGFP as a reporter, using the calcium-phosphate precipitation method.
• Vectors containing the transgene are concentrated by density purification over an iodixanol gradient (Axis-Shield, Oslo) and the 40% iodixanol fraction subsequently buffer exchanged by for example amicon filtration (Millipore).
• the AAV fraction is titered for DNase-resistant vector genomes by real-time PCR relative to a standard vector.
• 3 pi of viral vector are intravitreally injected into anaesthetized (100 mg/kg ketamine and 10 mg/kg xylazine) wild type C57BL/6J mice.
• the virus is titer-matched to E11 GC/ml and 3 ul thereof is injected - i.e. 3xE8 vg per eye.
• Mouse retinas are removed 3 weeks post-injection for immunohistochemical analysis.
• Mouse eyes retinal explants (7 days post-transduction) are fixed with 4% (wt/vol) paraformaldehyde in PBS for 40min at room temperature (RT), cryoprotected over three consecutive nights at 4°C in graded sucrose solutions (10%, 20%, and 30% sucrose in PBS), embedded in cryomolds with O.C.T. compound (Sakura Finetek), and frozen in liquid nitrogen-cooled 2-methylbutane.
• the reporter expression in the target cell type is quantified, typically by fluorescence, and compared to the amount of reporter in the remainder of the tissue to assess the biological activity of the engineered virus capsid.
• Another measure for biological activity of the engineered virus capsid is the percentage of target cells transduced and the area of tissue expressing. For the avoidance of doubt, the assay quantifying reporter expression in the target cell type and comparing to the amount of reporter in the remainder of the tissue is employed when measuring the biological activity.
• a second aspect of the invention relates to a nucleic acid sequence encoding the AAV capsid polypeptide according to the first aspect.
• the AAV sequence according to the invention does not contain a Rep element required for integration into a host genome. Non- integrating viruses are safer for application in humans.
• the nucleic acid sequence is designed according to the self-complementary AAV vector genome concept. In all cases a single stranded DNA sequence is used.
• the nucleic acid sequence comprises a transgene with or without regulatory sequences.
• the transgene encodes the sequence of a correct protein (i.e. RPE65), a siRNA or shRNA (designed to target regions of mRNA to degrade wild-type and mutant RNA of dominant disease-causing genes), or a CRISPR/Cas-gRNA cassette for gene editing.
• a correct protein i.e. RPE65
• siRNA or shRNA designed to target regions of mRNA to degrade wild-type and mutant RNA of dominant disease-causing genes
• CRISPR/Cas-gRNA cassette for gene editing a correct protein (i.e. RPE65), a siRNA or shRNA (designed to target regions of mRNA to degrade wild-type and mutant RNA of dominant disease-causing genes), or a CRISPR/Cas-gRNA cassette for gene editing.
• the transgene encodes a light-sensitive protein, such as an invertebrate or vertebrate opsin or a variant thereof.
• the transgene encodes channelrhodopsin-2 or a variant thereof.
• the transgene encodes a microbial light-gated inhibitory ion pump, such as for example halorhodopsin (eNpHR) or archaerhodopsin (ArchT).
• a microbial light-gated inhibitory ion pump such as for example halorhodopsin (eNpHR) or archaerhodopsin (ArchT).
• the transgene encodes a GPCR opsin such as for example human rhodopsin, melanopsin or a cone opsin.
• the transgene encodes an acceptor protein for a photo-switchable ligand.
• the transgene is under control of a promoter sequence operable in a mammalian cell.
• the promoter sequence is operable in a human retinal cell.
• the promoter is a ubiquitous promoter. In certain embodiments, the promoter is a cell-specific promoter.
• the promoter is a CMV immediate early promoter. In certain embodiments, the promoter is a human Ef1a promoter. In certain embodiments, the promoter is a photoreceptor specific promoter.
• a third aspect of the invention relates to an agent selected from the AAV capsid polypeptide according to the first aspect, an AAV vector comprising a capsid polypeptide according to the first aspect, and a nucleic acid sequence according to the second aspect, for use in medicine / as a medicament.
• AAV vectors are used to transduce cells of the eye.
• the eye is made up of three layers, composed of various anatomical structures.
• the fibrous tunic is the outermost layer and is composed of the cornea and sclera.
• the vascular tunic or uvea is the middle layer and consists of the choroid, ciliary body, pigmented epithelium and the iris.
• the retina is the innermost layer, which gets its oxygenation from the blood vessels of the choroid (posteriorly) as well as the retinal vessels (anteriorly).
• the spaces between the cornea and lens are filled with the aqueous humour, and the entire posterior cavity behind the lens is filled with the vitreous body, a jelly-like substance.
• the vitreous body is composed of water, collagen, fibrils, hyaluronic acid and ions.
• the void spaces in the retina that are not occupied by neurons or blood vessels; are filled by the processes of Muller glial cells that span all the retinal cell layers.
• the outer limiting membrane (OLM) of the retina is formed from junctions between Muller cells (MCs) and inner segments of photoreceptor cells and acts as a metabolic barrier between the subretinal space, restricting passage of large molecules.
• the inner limiting membrane (ILM) of the retina is formed by lateral connection between MCs end-feet and basement membranes and acts as a diffusion barrier between the vitreous humor and the neural retina.
• the retina is composed of macula, optic disc, fovea and peripheral retina.
• a photoreceptor cell is a specialized type of neuroepithelial cell found in the retina.
• Three types of photoreceptor cells are known: rods, cones, and photosensitive retinal ganglion cells.
• the rods are distributed at the peripheral region of the retina; whereas the central pigmented region called macula is enriched for cone photoreceptor cells.
• the retinal pigment epithelium (RPE) provides nutrition and maintains the health of photoreceptor cells.
• the nuclei of photoreceptor cells constitute the outer nuclear layer (ONL) whereas the nuclei of bipolar, amacrine and horizontal cells are located in the inner nuclear layer (INL).
• the agent selected from the AAV capsid polypeptide according to the first aspect, an AAV vector comprising a capsid polypeptide according to the first aspect, and a nucleic acid sequence according to the second aspect is for use in treatment of a condition affecting a. a retinal or retinal pigment epithelium cell and/or b. a photoreceptor, a bipolar cell, an amacrine cell or a ganglion cell of the retina.
• the agent is for use in treatment of glaucoma, retinitis pigmentosa, macular degeneration, retinoschisis, Leber' s Congenital Amaurosis, diabetic retinopathy, achromatopsia, or color blindness, melanoma-associated retinopathy, congenital stationary night blindness, cone-rod dystrophy, late stage age-related macular degeneration, maculopathies, early onset severe retinal dystrophy, achromatopsia, ocular albinism, oculocutaneous albinism, Stargardt disease, choroideremia, Spinocerebellar Ataxia type 7 (SCAT), lysosomal storage diseases that affect the cornea, such as Mucopolysaccharidosis (MPS) IV and MPS VII, retinoblastoma, ocular melanoma, hypertensive retinopathy.
• MPS Mucopolysaccharidosis
• MPS VII retinoblasto
• the agent of the invention can be employed for treatment or prevention of a disease affecting the inner ear.
• the agent is administered by a. intravitreal administration, particularly by intravitreal injection, or by b. subretinal administration.
• the agent is delivered to the posterior segment, the anterior segment, the sclera, the choroid, the conjunctiva, the iris, the lens, or the cornea.
• rod-cone dystrophies including retinitis pigmentosa
• cone-rod dystrophies including macular degeneration and congenital stationary night blindness (CSBN1)
• a dosage form for the prevention or treatment of a condition selected from cone-rod dystrophies, rod-cone dystrophies and congenital stationary night blindness comprising an AAV capsid and/or a nucleic acid sequence according to one of the above aspects of the invention.
• agents and methods disclosed herein do also provide significant advantages in early disease stages when damage to PR is small, as the vectors facilitated by the invention are more efficient than any existing alternatives.
• Fig. 1 Cell specificity of expression for selected capsid variants compared to the selection backbone AAV2 (Y252, 272, 444, 500, 700, 730F), referred to in the following as AAV2(M6), and the state-of-the-art synthetic capsid AAV2(7m8).
• All AAVs have been packaged as self-complementary (scAAVs) expressing either eGFP or mCitrine under two ubiquitous promoters hEF1a and CMV in order to remove the possible bias of one promoter towards a certain retinal cell type.
• scAAVs self-complementary
• hEF1a and CMV ubiquitous promoters
• Expression in photoreceptors (ONL) is significantly enhanced for the novel variants after intravitreal injection as opposed to AAV2(M6) and AAV2(7m8).
• titer 2.5 pi injected intravitreally, titer matched to 1E+11 vg/ml (except Cap14 at 3E+10). 4 retinas counted, mean ⁇ s.d.. INL, inner nuclear layer; GCL, ganglion cell layer; DAPI, number of all cell bodies stained with the nuclear stain DAPI.
• Fig. 2 Panretinal strong expression throughout the ONL for novel capsid variants as indicated at 100X reduced functional titer compared to state-of-the art AAV2(7m8). All injections were titer-matched and 7.4E+7 vg delivered into the vitreous of C57BL/6 mice. A.
• the AAV capsid is an icosahedron with 60 subunits.
• spiky protrusions peaks
• the highest peak is located at amino acid (AA) position 453 and the second highest at AA positions 587/588 (AAV2-VP1 numbering).
• AAV2(M6) six additional tyrosine to phenylalanine mutations (Y252,272,444,500,700,730F), referred to as AAV2(M6) (Table 2).
• Y-F and T-V mutations (Buning & Srivastava, Mol Ther Methods Clin Dev. 2019 Jan 26; 12:248-265.
• the dsDNA random oligonucleotides were cloned into the Asc ⁇ -Not ⁇ site of the modified AAV(M6) genome thereby replacing the stop codon.
• the virus library was generated such that each viral genome was packaged or encapsidated within the capsid protein variant which that genome encoded (genotype-phenotype coupling).
• all capsids were produced in a way that only one kind of peptide was present in each of the 60 subunits. In this way, functional improvements identified through selection can be linked to the genome sequence encoding this improved function contained within the viral capsid.
• This library was subjected to positive selection within C57BL/6_Opto-mGluR6 mice, generated by our laboratory (van Wyk et al., PLoS Biol, 2015. 13(5): p. e1002143), expressing the red fluorescent marker FP635 in retinal ON-bipolar cells.
• This mouse line was selected based on the rationale that ON-bipolar cells are located deep within the retina and are known not to be permissive to AAV transduction, so that the selection would favor well penetrating AAVs and variants with advantageous properties to transduce ON-bipolar cells.
• mice of 4-6 weeks of age were injected intravitreally with 2.5 pi of iodixanol-purified library with a genomic titer of approximately 5 x 10 11 viral genomes (vg)/ml into both eyes as described in (van Wyk et al., PLoS Biol, 2015. 13(5): p. e1002143; van Wyk et al., Front Neurosci, 2017.
• capsid genes of 81738 variants were identified by next- generation sequencing. 15 heptapeptide sequences from the top 67 ranked were selected for functional evaluation and characterization as outlined in table 1, on a combination of high overall transduction efficacy (accumulation in NGS), a preference for ON-bipolar cell targeting and motifs that either appeared early in the selection rounds or that looked promising in terms of their amino acid sequence (i.e. containing negatively charged residues or proline residues).
• a key characteristic of all library variants is the separation of the positive charges (Rsss and R588) of the heparan sulphate proteoglycan binding motif of AAV2 through the insertion.
• the peptide sequence together with the two alanine of the linker sequence can reinstall the ability to bind to heparan sulphate proteoglycan (Uhrig... Buning, Gene Therapy).
• the 7mer insert sequences there were moderate preferences at particular positions, e.g. charged amino acids at position 1 or 2 and/or at position 5 or 6 and a polar amino acid, such as Cys or Ala at position 7 (Table 1).
• a recombinant form of AAV2(M6) ⁇ 7mer ⁇ was cloned for all 15 selected peptides as of table 1.
• the inventors packaged with a scCMV-EGFP transgene (CMV: cytomegalovirus promoter). Except for two variants (Cap8 and Cap10), all capsids packaged well with titers between 10 11 - 10 12 vg/ml. Three weeks following intravitreal injections into adult mice, robust expression primarily in photoreceptors was observed for Cap3, Cap5, Cap7, Cap9, Cap 11, Cap 13 and Cap 14 (Table 1, Figures 1-3), but also some expression in cells of the inner retina such as bipolar cells, amacrine cells and Muller cells and very few retinal ganglion cells.
• AAV2(7m8) which is also an evolved AAV2 variant (Dalkara et al., Sci Transl Med, 2013. 5: p. 189ra76.) and currently considered state-of-the art for intravitreal injections
• novel variants penetrated significantly better though the retina and transduced significantly more photoreceptors and significantly less ganglion cells (Fig. 1, Figures 1-3).
• the new variants transduced photoreceptors in significantly larger number than the titer-matched controls AAV2(M6) and AAV(7m8). Consequently, the selected variants appear to possess superior inner limiting membrane and retinal penetration properties compared to AAV2(M6) and AAV2(7m8) and are significantly more effective in transducing the photoreceptors in the outer nuclear layer (ONL) (Fig. 1).
• Cap14 packaged with scCMV-EGFP and schEf1a-EGFP and injected at 3E+10 vg/ml performed significantly better than AAV2(7m8) injected at the same low dose (Figs. 1, 2) and even better than AAV2(7m8) at the minimal dose typically used in mouse of 1 E+12 vg/ml. This is promising in light of using lower doses in patients to reduce concerns of toxicity and immune responses.
• Table 1 Next-generation sequencing of ON-bipolar cell isolated variants from directed evolution revealed a high degree of convergence in viral libraries.
• The7mer inserts were flanked by alanine spacers in the form of ⁇ Ns87AAASASEASTAA Rsss- (SEQ ID NO 28) here exemplified for variant #3.
• ON-Bipolar cell dominance occurrence [ON-bipolar cells / Non-On-bipolar cells]
• Table 2 Amino acid sequences of AAV2 capsid protein VP1 with different insertions.
• VP3 grey sequence
• Y-F tyrosine to phenylalanine
• amino acid numbering refers to the whole VP1 sequence.
• the highest peak at G453 and the second highest peak at N587, where the random 7mers were inserted, are underlined and indicated by a white underlay. The insertion is in italics and boxed, including the alanine linkers.
• SI SEQ ID NO of the peptide insertion sequence
• FS SEQ ID NO of the full capsid sequence
• Table 3 Homo ogous positions of potential oligomer insertion sites and Y-F and T-V point mutation sites, respectively, on the capsid proteins of VP3 in different AAV serotypes.
• Capsid gene sequence (VP1) of adeno-associated virus 2 (Y252, 272, 444, 500, 700, 730 F), referred to as AAV2(M6)
• the Cap gene of the library consists of VP3 (grey sequence), tyrosine to phenylalanine (Y-F) mutations are underlined, base pair numbering refers to the whole VP1 sequence.

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