WO2021054763A1 - 향미증진제 및 이의 제조 방법 - Google Patents
향미증진제 및 이의 제조 방법 Download PDFInfo
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- WO2021054763A1 WO2021054763A1 PCT/KR2020/012618 KR2020012618W WO2021054763A1 WO 2021054763 A1 WO2021054763 A1 WO 2021054763A1 KR 2020012618 W KR2020012618 W KR 2020012618W WO 2021054763 A1 WO2021054763 A1 WO 2021054763A1
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- 239000012138 yeast extract Substances 0.000 description 1
- 239000011670 zinc gluconate Substances 0.000 description 1
- 235000011478 zinc gluconate Nutrition 0.000 description 1
- 229960000306 zinc gluconate Drugs 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/88—Taste or flavour enhancing agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/40—Meat products; Meat meal; Preparation or treatment thereof containing additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/40—Meat products; Meat meal; Preparation or treatment thereof containing additives
- A23L13/48—Addition of, or treatment with, enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/015—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
- A23L31/10—Yeasts or derivatives thereof
- A23L31/15—Extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/51—Concentration
Definitions
- the present application relates to a flavor enhancer for enhancing the flavor of meat and a method of manufacturing the same.
- Meat flavor can be realized through reactions between precursors such as amino acids, peptides, proteins, nucleic acids, fats, sugars and thiamine, and the Maillard reaction is an important reaction of these precursors to achieve meat flavor. It is being used as a reaction.
- the Mailerd reaction is a non-enzymatic browning reaction, which proceeds mainly with a nitrogen compound containing a reducing sugar and an amino group as a reactant, and a brown substance is formed through the reaction between them to give a characteristic odor. Conditions such as type have a great influence on the flavor of food.
- the present application is intended to provide a flavor enhancer with enhanced flavor of meat using natural materials.
- the present application is a material for manufacturing a flavor enhancer that can further enhance the flavor of meat by being used with a hydrolyzate of meat while being able to realize the flavor of meat by itself, a composition for manufacturing a flavor enhancer using the same, and flavors produced therefrom. We want to provide an enhancer.
- the present application is to provide a method of manufacturing a flavor enhancer that can shorten the manufacturing time of a flavor enhancer that realizes meat flavor, and improve the degree of meat flavor and problems of taste and off-flavor.
- one aspect of the present application includes a meat hydrolyzate containing amino acids and sugars, and a mushroom processed product, and the solid content of the mushroom processed product is 0.1 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate. It provides a composition for producing a flavor enhancer that contains in an amount of parts to 20 parts by weight.
- the present application provides a method of manufacturing a flavor enhancer comprising heating the composition for producing a flavor enhancer according to the present application.
- Another aspect of the present application provides a flavor enhancer comprising a pyrazine-based compound and acetic acid, wherein a content ratio of the pyrazine-based compound and the acetic acid is 1:1.5 to 1:2.5.
- composition for manufacturing a flavor enhancer of the present application When the composition for manufacturing a flavor enhancer of the present application is used, it is possible to provide a flavor enhancer having excellent consumer preference by improving taste, off-flavor, bitter taste, etc. while having excellent realization degree of yukhyang and preference for yukyang.
- a flavor enhancer having an improved content of a flavor component that can increase the flavor of beef and a flavor component that implements a umami taste.
- FIG. 2 is a graph showing a result of measuring the content of a pyrazine-based compound when analyzing volatile components for the flavor enhancers of Comparative Examples 1 and 2 and Example 1 prepared in Preparation Example 1.
- FIG. 2 is a graph showing a result of measuring the content of a pyrazine-based compound when analyzing volatile components for the flavor enhancers of Comparative Examples 1 and 2 and Example 1 prepared in Preparation Example 1.
- FIG. 3 is a graph showing a result of measuring the content of fragrance components other than pyrazine-based compounds when analyzing volatile components for the flavor enhancers of Comparative Examples 1 and 2 and Example 1 prepared in Preparation Example 1.
- FIG. 3 is a graph showing a result of measuring the content of fragrance components other than pyrazine-based compounds when analyzing volatile components for the flavor enhancers of Comparative Examples 1 and 2 and Example 1 prepared in Preparation Example 1.
- FIG. 4 is a graph showing a result of measuring the content of a pyrazine-based compound when analyzing volatile components for the flavor enhancers of Comparative Examples 3 and 4 prepared in Preparation Example 1.
- FIG. 4 is a graph showing a result of measuring the content of a pyrazine-based compound when analyzing volatile components for the flavor enhancers of Comparative Examples 3 and 4 prepared in Preparation Example 1.
- FIG. 5 is a graph showing a result of measuring the content of acetic acid when analyzing volatile components for the flavor enhancers of Comparative Example 1 and Example 1 prepared in Preparation Example 1.
- FIG. 5 is a graph showing a result of measuring the content of acetic acid when analyzing volatile components for the flavor enhancers of Comparative Example 1 and Example 1 prepared in Preparation Example 1.
- FIG. 6 is a graph showing the results of measuring the contents of aspartic acid and glutamic acid, which are umami components in the flavor enhancers of Comparative Examples 1 and 2 and Example 1 prepared in Preparation Example 1.
- FIG. 6 is a graph showing the results of measuring the contents of aspartic acid and glutamic acid, which are umami components in the flavor enhancers of Comparative Examples 1 and 2 and Example 1 prepared in Preparation Example 1.
- FIG. 7 is a graph showing the results of measuring the content of amino acids other than aspartic acid and glutamic acid in the flavor enhancers of Comparative Examples 1 and 2 and Example 1 prepared in Preparation Example 1.
- FIG. 7 is a graph showing the results of measuring the content of amino acids other than aspartic acid and glutamic acid in the flavor enhancers of Comparative Examples 1 and 2 and Example 1 prepared in Preparation Example 1.
- FIG. 10 is a graph showing a result of measuring the content of acetic acid when analyzing volatile components of the flavor enhancer prepared in Preparation Example 2;
- FIG. 11 is a graph showing the results of measuring the content of aspartic acid and glutamic acid, which are umami components in the flavor enhancer prepared in Preparation Example 2.
- FIG. 11 is a graph showing the results of measuring the content of aspartic acid and glutamic acid, which are umami components in the flavor enhancer prepared in Preparation Example 2.
- composition for producing a flavor enhancer of the present application will be described in detail.
- composition for producing a flavor enhancer of the present application includes a meat hydrolyzate and a mushroom processed product.
- the flavor enhancer of the present application is a substance that enhances flavor and flavor, and may be used in combination with flavoring agents, sauces, and seasonings.
- the "hydrolyzate” is prepared by hydrolyzing meat, and includes amino acids and sugars.
- the meat may be at least one selected from the group consisting of beef, pork, lamb, goat, venison, chicken, duck, and turkey. Not limited.
- the meat may include beef, and may include at least one portion selected from the group consisting of beef loin, sirloin, chamois, beef tongue, tenderloin, forelimbs, ribs, yangji, suldo and sedam, and , For example, may include events.
- the meat hydrolysis method may be a method known as protein hydrolysis, such as an enzyme, acid or alkali hydrolysis method, without limitation, and specifically, may be a hydrolysis method using an enzyme.
- the enzyme is not particularly limited as long as it is an enzyme capable of hydrolyzing animal proteins, for example, pancreatin, pineapple extract protease, papaya extract protease, kiwi extract protease, Bacillus li Proteolytic enzymes derived from Bacillus licheniformis (e.g. Alcalase ® ), proteolytic enzymes derived from Aspergillus oryzae (e.g.
- Flavourzyme ® ⁇ -chymotrypsin, papain, trypsin acetylated, ficin, thermolysin, pepsin, trypsin, serine protease ( serine proteases, threonine proteases, cysteine proteases, aspartate proteases, glutamic proteases, and metalloproteases It may include at least one selected from the group consisting of.
- the enzyme may be at least one of pancreatin and a proteolytic enzyme derived from Aspergillus oris.
- the hydrolysis temperature is not particularly limited as long as the hydrolytic enzyme using the enzyme exhibits hydrolytic activity, but may be specifically 30°C to 60°C or 40°C to 55°C.
- the hydrolysis can be carried out until the end of the desired reaction, but is not particularly limited, for example, 30 minutes to 20 hours, 1 hour to 10 hours, 1 hour to 5 hours, 1 hour to 4 hours or 2 hours It can be carried out for 3 hours.
- the enzyme may be used in a conventional amount used for protein hydrolysis of meat, and the amount is not particularly limited, but, for example, 0.01 to 5 parts by weight, specifically 0.1 to 5 parts by weight based on 100 parts by weight of meat. It can be used in parts by weight, 1 to 5 parts by weight, or 2 to 3 parts by weight.
- the meat hydrolyzate prepared as described above is not limited thereto, but the solid content may be 5 to 40 Brix%, for example, 5 to 35 Brix%, 5 to 30 Brix%, 5 to 25 Brix%, 5 To 20 Brix%, or 10 to 15 Brix%.
- the flavor enhancer manufactured using the meat hydrolyzate may be preferable in terms of the degree of implementation of the meat flavor and the manufacturing cost.
- the brix of the present application represents the amount of solids contained in 100 g of a solution as the number of grams based on saccharides, and may be used interchangeably with brix%, bx, and the like.
- the brix may be measured by a known method, and may be measured at room temperature such as 15°C to 35°C.
- the brix may be the amount of solids contained in 100 g of water expressed as the number of grams based on sugar.
- the solid content may contain amino acids and sugars, and the amino acids may include at least one selected from the group consisting of arginine, leucine, phenylalanine, glutamic acid, aspartic acid, and salts thereof.
- the meat hydrolyzate may contain at least one of glutamic acid, aspartic acid, and salts thereof as the amino acid for enhancing the flavor of meat.
- the salt of the amino acid is not limited thereto as a salt of the amino acid, and for example, represents a form that can be used in food such as sodium salt, magnesium salt, potassium salt, calcium salt, hydrochloride, ammonium salt of the amino acid.
- the arginine may be included in an amount of 10 to 20 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate, and specifically, may be included in an amount of 12 to 18 parts by weight, or 14 to 16 parts by weight.
- the leucine may be included in an amount of 10 to 20 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate, and specifically, may be included in an amount of 11 to 18 parts by weight, or 12 to 15 parts by weight.
- the phenylalanine may be included in an amount of 5 to 15 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate, and specifically, may be included in an amount of 8 to 13 parts by weight, or 10 to 12 parts by weight.
- the glutamic acid may be included in an amount of 0.1 to 5 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate, and specifically, may be included in an amount of 1 to 3 parts by weight.
- the aspartic acid may be included in an amount of 0.01 to 1 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate, and specifically, may be included in an amount of 0.01 to 0.05 parts by weight.
- the saccharide may contain at least one of glucose, maltose, sucrose, and fructose, and the composition for preparing the flavor enhancer contains 100 to 600 parts by weight of the saccharide based on 100 parts by weight of the amino acid, for example, 150 to 500 It may be included in an amount of 200 to 460 parts by weight, 200 to 400 parts by weight, or 230 to 300 parts by weight.
- the content ratio of the amino acid and the saccharide is within the above range, it may be preferable in terms of the degree of improvement in the flavor of the flavor enhancer prepared using the same and in terms of nutrition enhancement by the flavor enhancer.
- the glucose may be included in an amount of 10 to 20 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate, and specifically, may be included in an amount of 11 to 18 parts by weight, or 12 to 15 parts by weight.
- the maltose may be included in an amount of 5 to 15 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate, and specifically, may be included in an amount of 8 to 14 parts by weight, or 10 to 13 parts by weight.
- the sucrose may be included in an amount of 0.1 to 5 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate, and specifically 1 to 4 parts by weight, or 2 to 3.5 parts by weight.
- the fructose may be included in an amount of 0.1 to 5 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate, and specifically 1 to 4 parts by weight, or 2 to 3.5 parts by weight.
- the meat hydrolyzate as described above may be included in an amount of 20 to 80 parts by weight of the solid content of the meat hydrolyzate based on 100 parts by weight of the solid content of the composition for producing the flavor enhancer, for example, 20 to 60 parts by weight, 22 to 50 parts by weight, 23 It may be included in an amount of from 40 to 40 parts by weight or from 25 to 30 parts by weight, but is not limited thereto.
- the mushroom processed product may be included without particular limitation, as long as it is a material containing a component of the mushroom, the method of obtaining it, the component, the form, and the like.
- the method of obtaining the mushroom processed product may be processed according to a known food processing method such as pulverization, juice, fermentation, extraction, concentration, etc., and is not particularly limited, but may be specifically prepared by heating a mushroom extract.
- a known food processing method such as pulverization, juice, fermentation, extraction, concentration, etc.
- it may be prepared by mixing purified salt, fish fermentation broth, etc. to a reaction raw material containing at least one of a mushroom raw material, a mushroom extract, and a concentrate of a mushroom extract, and then reacting the mixed raw materials at a temperature of 110°C to 130°C.
- the mushroom processed product may serve to further improve the meat flavor through a chemical reaction with the component of the meat hydrolyzate.
- the mushroom may be obtained, for example, from at least one selected from the group consisting of matsutake, bird matsutake, mushroom, shiitake, oyster and wood ear.
- the mushroom processed product prepared as described above is not limited thereto, for example, the solid content may be 20 to 70 brix%, for example 25 to 65 brix%, 30 to 60 brix%, 35 to 55 brix% , 40 to 50 brix%, or 35 to 45 brix%.
- the content of the solid content is within the above range, it may be preferable in terms of improving the degree of improvement of the meat flavor and flavor such as umami of the flavor enhancer prepared using the mushroom processed product.
- the solid content of the mushroom processed product may contain an amino acid, and the amino acid may include at least one selected from the group consisting of aspartic acid, glutamic acid, threonine, serine, tyrosine, lysine, and salts thereof.
- the mushroom processed product may include at least one of aspartic acid, glutamic acid, and salts thereof as the amino acid for enhancing the flavor of meat in the flavor enhancer of the present application.
- the salt of the amino acid is not limited thereto as a salt of the amino acid, and for example, represents a form that can be used in food such as sodium salt, magnesium salt, potassium salt, calcium salt, hydrochloride, ammonium salt of the amino acid.
- the aspartic acid and its salt are 1 to 10 parts by weight, 1.5 to 8 parts by weight, 2 to 6 parts by weight, 2 to 4 parts by weight, or 2.5 to 3.8 parts by weight based on 100 parts by weight of the solid content of the mushroom processed product. It can be included in the content.
- the glutamic acid and its salt may be included in an amount of 1 to 10 parts by weight, 3 to 9 parts by weight, 5 to 8 parts by weight, 6 to 8 parts by weight, or 6.25 to 7.5 parts by weight based on 100 parts by weight of the solid content of the mushroom processed product. .
- the threonine and its salt may be included in an amount of 0.1 to 10 parts by weight, 0.5 to 5 parts by weight, 0.8 to 1.5 parts by weight, or 1 to 1.1 parts by weight based on 100 parts by weight of the solid content of the mushroom processed product.
- the serine and its salt may be included in an amount of 0.1 to 10 parts by weight, 0.5 to 5 parts by weight, 1 to 1.5 parts by weight, or 1.1 to 1.3 parts by weight based on 100 parts by weight of the solid content of the mushroom processed product.
- the tyrosine and its salt may be included in an amount of 0.1 to 10 parts by weight, 0.5 to 5 parts by weight, 1 to 1.5 parts by weight, or 1.1 to 1.3 parts by weight based on 100 parts by weight of the solid content of the mushroom processed product.
- the lysine and its salt may be included in an amount of 0.1 to 10 parts by weight, 0.5 to 5 parts by weight, 0.5 to 1 part by weight, or 0.8 to 0.9 parts by weight based on 100 parts by weight of the solid content of the mushroom processed product.
- the mushroom processed product may include a pyrazine-based compound and acetic acid.
- the content ratio of the pyrazine-based compound and the acetic acid is not limited thereto, but may be included as 1:1 to 1:3, 1:1.5 to 1:2.8, or 1:2 to 1:2.5.
- the pyrazine-based compound is not limited thereto, for example, methylpyrazine; Dimethylpyrazine such as 2,5-dimethylpyrazine and 2,6-dimethylpyrazine; Ethylmethylpyrazine such as 2-ethyl-5-methylpyrazine and 2-ethyl-6-methylpyrazine; It may include at least one selected from the group consisting of 2,3,5-trimethylpyrazine, and specifically, in expressing the content of the acetic acid, 100 parts by weight of the pyrazine-based compound is methylpyrazine, 2,5-dimethylpyrazine, It is taken as the sum of the contents of each of 2,6-dimethylpyrazine, 2-ethyl-6-methylpyrazine, and 2,3,5-trimethylpyrazine.
- the ratio of the pyrazine-based compound and the methylpyrazine content is not limited thereto, but may be included in the range of 1:0.2 to 1:0.5.
- the pyrazine-based compound and the methylpyrazine content ratio may be 1:0.25 to 1:0.4, 1:0.25 to 1:0.35, or 1:0.28 to 1:0.33.
- the content of volatile components such as pyrazine-based compounds and acetic acid in the mushroom processed product of the present application is preferably based on a value measured when the content of the volatile component of the flavor enhancer is analyzed.
- the mushroom processed product and/or its volatile component may further include at least one of dimethyl disulfide, 2,4,5-trimethyloxazole, 2-pentyl furan, and 2-acetylfuran, and specifically, the mushroom processed product and / Or its volatile components may be those containing dimethyl disulfide, 2,4,5-trimethyloxazole, 2-pentyl furan and 2-acetylfuran.
- the composition for producing the flavor enhancer may include a solid content of the mushroom processed product in an amount of 0.1 parts by weight to 20 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate.
- the solid content of the mushroom processed product is 0.1 to 20 parts by weight, 0.1 to 18 parts by weight, 0.1 to 16 parts by weight, 0.1 to 14 parts by weight based on 100 parts by weight of the solid content of the meat hydrolyzate.
- the degree of implementing the fleshy flavor of the flavor enhancer manufactured using it and/or reducing the taste and off-flavor may be more excellent, and the flavor of the umami taste may be further improved. It can have the effect of becoming.
- the composition for producing the flavor enhancer may further include a metal salt for saltiness in addition to the meat hydrolyzate and mushroom processed product.
- the metal salt may be used without limitation as long as it is a material that gives a taste such as saltiness, and specifically, may be at least one of a sodium salt and a potassium salt. More specifically, it may be at least one of sodium chloride, potassium chloride, and potassium lactate.
- the amount of the metal salt can be adjusted as needed, but is not limited thereto, for example, the solid content of the metal salt is 5 to 25% by weight, such as 7 to 20% by weight, 10 to 18, based on the total weight of the solid content of the composition for preparing the flavor enhancer. It may be included in an amount of 15 to 18% by weight. By including it in the above range, the effect of enhancing flavor can be further improved.
- composition for preparing the flavor enhancer may further include maltodextrin and/or modified starch.
- the amount of maltodextrin may be adjusted as needed, but the amount of maltodextrin may be included in an amount of 0.1 to 20% by weight based on the total weight of the composition for preparing the flavor enhancer, for example, For example, it may be included in an amount of 1 to 19% by weight, 3 to 18% by weight, 5 to 17% by weight, 7 to 15% by weight, 9 to 13% by weight, or 10 to 12% by weight.
- the amount of the modified starch may be adjusted as needed, and the amount of the modified starch may be included in an amount of, for example, 0.01 to 10% by weight based on the total weight of the composition for producing the flavor enhancer, For example, it may be included in an amount of 0.01 to 5% by weight, 0.05 to 3% by weight, or 0.1 to 2% by weight.
- composition for producing the flavor enhancer may not include other additives other than the above-described components, or may further include other additives.
- other additives may include excipients acceptable for food, and excipients acceptable for food are not limited thereto, for example, sodium cross-linked carboxymethylcellulose, gatigum, and reduced pigment.
- Licorice extract formic acid, geranyl formic acid, citronelyl formic acid, isoamyl formic acid, gum resin, geraniol, crystalline cellulose, cinnamic acid, methyl cinnamon, ethyl cinnamate, aldehyde cinnamon, cinnamon alcohol, high pigment, benzoyl peroxide, Hydrogen peroxide, peracetic acid, ammonium persulfate, guar gum, 5'-disodium guanylate, citric acid, manganese citrate, trisodium citrate, sodium ferrous citrate, iron citrate, ammonium iron citrate, potassium citrate, calcium citrate, magnesium silicate, calcium silicate , Silicon resin, diatomaceous earth, gluconic acid, sodium gluconate, copper gluconate, magnesium gluconate, manganese gluconate, zinc gluconate, iron gluconate, potassium gluconate, calcium gluconate, glutaminase,
- the composition for manufacturing a flavor enhancer when considering the amount of meat used, the loss of meat flavor is reduced, while enhancing the inherent flavor of meat, reducing the taste and odor, and having excellent overall flavor and umami characteristics. It can exhibit the effect of manufacturing a flavor enhancer.
- the present application provides a method of manufacturing a flavor enhancer using the composition for preparing the flavor enhancer.
- the method for preparing the flavor enhancer includes heating the composition for preparing the flavor enhancer. Specifically, it may include a step of heat-treating the composition for producing the flavor enhancer under pressurized conditions (hereinafter, also referred to as'high temperature/high pressure treatment').
- the composition for producing a flavor enhancer is heated or treated with high temperature and high pressure, it may exhibit an effect of removing an image or an off-flavor in the composition for producing a flavor enhancer.
- the composition for producing the flavor enhancer is heated or treated with high temperature and high pressure, the flavor of meat can be enhanced through a chemical reaction between the components in the composition for producing the flavor enhancer, and the effect of reducing taste and off-flavor can be exhibited.
- the mechanism is not limited thereto.
- the heating or high temperature/high pressure treatment may be carried out under conditions capable of volatilizing substances that cause an off-flavor, such as acetic acid, in the composition for preparing the flavor enhancer, and induces a chemical reaction between the components, such as a Mailard reaction. It can be carried out under the conditions for.
- an off-flavor such as acetic acid
- the heat treatment may be performed at a temperature of 100°C or higher, for example, 100°C to 130°C or 110°C to 125°C.
- the heat treatment may be performed under pressure conditions, for example, at a pressure of 1 kgf/cm 2 to 1.5 kgf/cm 2 , for example, 1.2 kgf/cm 2 to 1.4 kgf/cm 2 It can be carried out under pressurized conditions with a pressure of
- It can be carried out for 10 minutes to 60 minutes, for example 20 minutes to 40 minutes under the above temperature and/or pressure conditions. It may be advantageous in achieving the effect of enhancing the flavor of meat and further reducing the taste and off-flavor by performing high temperature and high pressure treatment under the above conditions.
- the method for preparing the flavor enhancer may further include obtaining a concentrate of the composition for preparing the flavor enhancer by concentrating the composition for preparing the flavor enhancer before the heating or high temperature/high pressure treatment. Accordingly, the heating or high temperature/high pressure treatment may be performed on the concentrate of the composition for producing the flavor enhancer to further increase process efficiency.
- the step of obtaining the concentrate of the composition for producing the flavor enhancer is not particularly limited as long as it is a method commonly used in food processing processes, and for example, evaporation concentration, freeze concentration, distillation, membrane separation concentration, vacuum concentration, etc. It may be performed, and the concentration step may be performed before or after the heat treatment (or high temperature/high pressure treatment), or at the same time.
- the composition for producing the flavor enhancer may be concentrated in a vacuum by stirring at 50 ⁇ 5 rpm at a condition of 670 mmHh or more and a temperature of 55 ⁇ 5°C.
- the concentrate of the composition for preparing the flavor enhancer may be one obtained by concentrating the composition for preparing the flavor enhancer at 2 to 10 magnification, for example, 3 to 6 magnification, or 4 to 5 magnification.
- the concentrate of the composition for producing a flavor enhancer may have a solid content of 10 to 80 Brix%, for example, 20 to 75 Brix%, 30 to 70 Brix%, 40 to 65 Brix%, 50 to 65 Brix % Or 55 to 60 Brix%.
- Flavor enhancers of the present application include pyrazine-based compounds and acetic acid.
- the flavor enhancer may be prepared from the composition for producing the flavor enhancer of the present application, may be obtained according to the method for preparing the flavor enhancer, and may be obtained in the form of a composition.
- the pyrazine-based compound may be included as one of the flavor components that realize the flavor of meat, and the acetic acid may be included as one of the components exhibiting a unique taste and off-flavor.
- the content ratio of the pyrazine-based compound and the acetic acid is not limited thereto, but is 1:1, 1:1.2, 1:1.5, 1:1.55, 1:1.6, 1:1.65, 1:1.7, and 1 :
- the content ratio of the pyrazine-based compound and the acetic acid may be 1:1 to 1:3, 1:1.20, 1:3, 1:1.2 to 1:2.2, or 1:1.5 to 1:2.1.
- the acetic acid when included in the above amount, it may exhibit an excellent effect of improving the taste and off-flavor of the flavor enhancer.
- the flavor of the hexahedron when included in the content ratio, the flavor of the hexahedron may be excellent, and the degree of reduction of the taste and off-flavor may be more excellent.
- the content of the pyrazine-based compound in the total amount of volatile components may be 2% or more.
- the content of the pyrazine-based compound among the total volatile component content may be, for example, 2% to 6%, 2.5% to 5.5%, 3% to 5%, or 3% to 4.5%.
- The% may be expressed as a content %.
- the pyrazine-based compound included in the volatile component is not limited thereto, but examples thereof include methylpyrazine; Dimethylpyrazine such as 2,5-dimethylpyrazine and 2,6-dimethylpyrazine; Ethylmethylpyrazine such as 2-ethyl-5-methylpyrazine and 2-ethyl-6-methylpyrazine; It may include at least one selected from the group consisting of 2,3,5-trimethylpyrazine, and specifically, in expressing the content of the acetic acid, 100 parts by weight of the pyrazine-based compound is methylpyrazine, 2,5-dimethylpyrazine, It is taken as the sum of the contents of each of 2,6-dimethylpyrazine, 2-ethyl-6-methylpyrazine, and 2,3,5-trimethylpyrazine.
- the content ratio of the pyrazine-based compound and the methylpyrazine is not limited thereto, but may be included in the range of 1:0.2 to 1:0.5.
- the content ratio of the pyrazine-based compound and the methylpyrazine may be 1:0.25 to 1:0.4, 1:0.25 to 1:0.35, or 1:0.25 to 1:0.3.
- the content of volatile components such as pyrazine-based compounds and acetic acid in the flavor enhancer of the present application may be based on a value measured when the content of the volatile component of the flavor enhancer is analyzed.
- the method of analyzing the content of the volatile component is not particularly limited as long as it is a known method, and for example, solid chromatography, liquid chromatography, high performance liquid chromatography, gas chromatography, ion chromatography, gel permeation chromatography
- the measurement may be performed using a method such as a graph, and more specifically, it may be based on a measured value measured using gas chromatography together with a mass spectrometer.
- the content ratio, or content%, in the present application may be a weight ratio, or weight%, between materials to be compared, and may be a molar ratio, or a mole%.
- the content ratio or content% may be an area ratio of a peak in an analysis result, an area ratio% of a peak, a height ratio of a peak in an analysis result, or a height ratio% of a peak.
- the content ratio or the content% may be derived by comparing the area ratio of the peak of the retention time of the material analyzed using gas chromatography or the area ratio% to the total area.
- the content of the volatile component may be measured with respect to the diluted product of the flavor enhancer in order to increase the accuracy of quantitative analysis of the volatile component.
- the flavor enhancer it is possible to measure the content of the volatile component by putting the flavor enhancer in a container of about 10 times.
- 1 g of the flavor enhancer may be placed in a 10 mL container or 2 g of the flavor enhancer may be placed in a 20 mL container to measure the content of volatile components.
- the flavor enhancer and/or volatile component thereof may further include at least one of dimethyl disulfide, 2,4,5-trimethyloxazole, 2-pentyl furan, and 2-acetylfuran, and specifically, the flavor enhancer and / Or its volatile components may be those containing dimethyl disulfide, 2,4,5-trimethyloxazole, 2-pentyl furan and 2-acetylfuran.
- the content ratio of the pyrazine-based compound and the dimethyl disulfide is not limited thereto, but one lower limit selected from the group consisting of 1:0.1, 1:0.15 and 1:0.2 and/or 1:0.5, 1:0.45, 1 It can be included in a content range consisting of one upper limit selected from the group consisting of :0.4, 1:0.35, 1:0.3 and 1:0.25.
- the content ratio of the pyrazine-based compound and the dimethyl disulfide is 1:0.1 to 1:0.5, 1:0.1 to 1:0.45, 1:0.11:0.4, 1:0.1 to 1:0.35, 1:0.1 to 1 It may be included as :0.3, 1:0.1 to 1:0.25, 1:0.15 to 1:0.25, or 1:0.2 to 1:0.25.
- the content ratio of the pyrazine-based compound and the 2,4,5-trimethyloxazole is not limited thereto, but one lower limit selected from the group consisting of 1:0.1, 1:0.15 and 1:0.2 and/or 1 :0.5, 1:0.45, 1:0.4, 1:0.35, 1:0.3 and 1:0.25 may be included in a content range consisting of one upper limit selected from the group consisting of parts by weight.
- the content ratio of the pyrazine-based compound and the 2,4,5-trimethyloxazole is 1:0.1 to 1:0.5, 1:0.1 to 1:0.45, 1:0.1 to 1:0.4, 1:0.1 to It may be included as 1:0.35, 1:0.1 to 1:0.3, 1:0.1 to 1:0.25, 1:0.15 to 1:0.25 or 1:0.2 to 1:0.25.
- the content ratio of the pyrazine-based compound and the 2-pentyl furan is not limited thereto, but one lower limit selected from the group consisting of 1:0.1, 1:0.15 and 1:0.2 and/or 1:0.5, 1:0.45 , 1:0.4, 1:0.35, 1:0.3 and 1:0.25 can be included in a content range consisting of one upper limit selected from the group consisting of.
- the content ratio of the pyrazine-based compound and the 2-pentyl furan is 1:0.1 to 1:0.5, 1:0.1 to 1:0.45, 1:0.1 to 1:0.4, 1:0.1 to 1:0.35, 1 It may be included as :0.1 to 1:0.3, 1:0.1 to 1:0.25, 1:0.15 to 1:0.25, or 1:0.2 to 1:0.25.
- the content ratio of the pyrazine-based compound and the 2-acetylfuran is not limited thereto, but the group consisting of 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.1, 1:1.2 and 1:1.3 It may be included in a content range consisting of one lower limit selected from and/or one upper limit selected from the group consisting of 1:2.2, 1:2, 1:1.8, 1:1.6, 1:1.5 and 1:1.4. .
- the content ratio of the pyrazine-based compound and the 2-acetylfuran is 1:0.7 to 1:2.2, 1:0.8 to 1:2, 1:0.9 to 1:1.8, 1:1 to 1:1.6, 1 It may be included as :1.1 to 1:1.5, 1:1.2 to 1:1.4 or 1:1.3 to 1:1.4.
- the flavor enhancer of the present application may include at least one of glutamic acid, aspartic acid, or salts thereof.
- the salts of amino acids such as glutamic acid and aspartic acid are in the form of salts of the amino acids and are not limited thereto, and for example, sodium salt, magnesium salt, potassium salt, calcium salt, hydrochloride, ammonium salt of the amino acid can be used in food. It represents the form.
- the content of glutamic acid or a salt thereof in the flavor enhancer may be 14 parts by weight or more based on 100 parts by weight of amino acids in the flavor enhancer.
- the content of glutamic acid or a salt thereof is selected from the group consisting of 14 parts by weight, 14.2 parts by weight, 15 parts by weight, 16 parts by weight, 16 parts by weight, 17 parts by weight, and 17.5 parts by weight based on 100 parts by weight of amino acids in the flavor enhancer.
- One lower limit and/or its upper limit is not particularly limited, but, for example, the group consisting of 50 parts by weight, 40 parts by weight, 35 parts by weight, 30 parts by weight, 25 parts by weight and 20 parts by weight based on 100 parts by weight of amino acids in the flavor enhancer.
- the content of the aspartic acid or salt thereof in the flavor enhancer may be 2.5 parts by weight or more based on 100 parts by weight of amino acids in the flavor enhancer.
- the content of the aspartic acid or salt thereof is specifically limited to one lower limit and/or its upper limit selected from the group consisting of 2.5 parts by weight, 2.7 parts by weight, 2.9 parts by weight, 3.0 parts by weight, 3.1 parts by weight and 3.2 parts by weight.
- it may be a content range consisting of one upper limit selected from the group consisting of 20 parts by weight, 15 parts by weight, 10 parts by weight, and 5 parts by weight.
- based on 100 parts by weight of amino acids in the flavor enhancer 2.5 to 20 parts by weight, 2.5 to 15 parts by weight, 2.7 to 15 parts by weight, 2.9 to 15 parts by weight, 3.1 to 15 parts by weight 3.1 to 10 parts by weight, 3.2 to 10 It may be included in an amount of 3.2 to 5 parts by weight or 3.2 to 5 parts by weight.
- the flavor enhancer may further include other amino acids or salts thereof in addition to glutamic acid, aspartic acid or salts thereof, such amino acids are not limited thereto, for example, at least one of threonine, serine, tyrosine and lysine It may be, and more specifically, may include threonine, serine, tyrosine and lysine.
- the content of threonine or a salt thereof in the flavor enhancer may be 3 parts by weight or more based on 100 parts by weight of amino acids in the flavor enhancer.
- the content of threonine or a salt thereof may be 3 parts by weight to 5 parts by weight or 3 parts by weight to 3.5 parts by weight.
- the content of the serine or salt thereof in the flavor enhancer may be 3 parts by weight or more based on 100 parts by weight of amino acids in the flavor enhancer.
- the content of the serine or salt thereof may be 3 parts by weight to 5 parts by weight or 3 parts by weight to 3.5 parts by weight.
- the content of the tyrosine or salt thereof in the flavor enhancer may be 4.5 parts by weight or more based on 100 parts by weight of amino acids in the flavor enhancer.
- the content of the tyrosine or salt thereof may be 4.5 to 6 parts by weight or 5 to 5.5 parts by weight.
- the content of the lysine or salt thereof in the flavor enhancer may be 4.5 parts by weight or more based on 100 parts by weight of amino acids in the flavor enhancer.
- the content of lysine or a salt thereof may be 4.5 to 6 parts by weight or 5 to 5.5 parts by weight.
- the beef hydrolyzate was prepared by treating the beef with a hydrolyzate after cutting the beef, and the composition of the prepared beef hydrolyzate was shown in Table 1 below.
- Mushroom extract concentrate 30%, purified salt 15%, fish fermentation broth 12%, and other additives 43% were mixed with purified water to prepare a mushroom processed raw material prepared by reacting at a temperature of 120 °C 30 minutes to prepare a mushroom processed product.
- the solid content of the mushroom processed product prepared as described above was 40 bx%, and the composition of the volatile component of the prepared mushroom processed product is shown in Table 2 below, and the amino acid composition is shown in FIG. 1.
- Volatile components of the mushroom processed product were placed in a 20 mL vial in 2 g of the mushroom processed product prepared above, and the components volatilized in the gas space (head space of the vial) were collected in the vial, and the composition was analyzed using gas chromatography.
- the content (%) represents the measurement area of each component in% of the total measurement area of gas chromatography.
- the prepared beef hydrolyzate and mushroom processed product, maltodextrin, salt, and other additives were uniformly mixed in the composition of Table 3 to prepare a composition for producing a flavor enhancer.
- Comparative Examples 1 and 2 were prepared so as not to include the mushroom processed product, and in Comparative Example 2, 5 known as a flavor enhancer by replacing the mushroom processed product.
- IMP Disodium Inosine
- composition for preparing the flavor enhancer of Comparative Examples 1 and 2 and Example 1 prepared above was concentrated at 4.44 magnification by vacuum concentration to obtain a concentrate.
- the obtained concentrate was subjected to high temperature and high pressure treatment, and the high temperature and high pressure treatment conditions are shown in Table 4 below.
- Comparative Example 3 without high-temperature and high-pressure treatment after concentration and Table 4 after concentration. Comparative Example 4 in which only minimal sterilization was performed by heating at 90° C. for 10 minutes without high temperature and high pressure treatment was prepared together.
- the identification of volatile components was performed by comparing the MS values based on the Library shown in Table 5, and by confirming the RI values obtained through experiments and the RI values reported in the literature.
- Volatile component concentration (area of the volatile component)/(total area of the identified volatile component)
- FIG. 4 shows the results of analyzing the content of the pyrazine-based compound for Comparative Examples 3 and 4
- FIG. 5 shows the results of analyzing the content of acetic acid for Comparative Examples 1 and 1.
- Example 1 is excellent in terms of the content of the pyrazine-based compound compared to Comparative Example 1 in which the mushroom processed product was not used during the manufacture of the flavor enhancer, and a pyrazine-based level similar to that of Comparative Example 2 using IMP. It was confirmed that the compound was contained. In addition, as can be seen in Figure 3, it was confirmed that Example 1 is excellent in terms of the content of fragrance components other than the pyrazine-based compound compared to Comparative Examples 1 and 2.
- Example 1 if the composition of Example 1 is not subjected to high temperature and high pressure treatment, or if the reaction of the components in the composition for producing a flavor enhancer does not occur sufficiently, the flavor enhancer according to the present application is not prepared and It was confirmed that the effect of enhancement was poor.
- the amino acid composition in the flavor enhancer is an amino acid analyzer (L-8900 amino acid analyzer with two single channel colorimeters, column: 2622SC-PH ion exchange column (4.6X60mm, Hitachi, Co., Jap), solution: sodium acetate buffer (MCI buffer PH1). , PH4, RG), Ninhydrin solution (R1, R2)).
- L-8900 amino acid analyzer with two single channel colorimeters, column: 2622SC-PH ion exchange column (4.6X60mm, Hitachi, Co., Jap), solution: sodium acetate buffer (MCI buffer PH1). , PH4, RG), Ninhydrin solution (R1, R2)).
- Example 1 was superior to Comparative Examples 1 and 2 in terms of the content of glutamic acid and aspartic acid, which are umami components.
- Example 1 was superior to Comparative Examples 1 and 2 in terms of the content of amino acids such as threonine, serine, tyrosine and lysine in addition to the above two amino acids. .
- a flavor enhancer was prepared in the same manner as in Preparation Example 1, except that the composition for producing a flavor enhancer was blended in the composition shown in Table 6 below.
- the content of the mushroom processed product was mixed differently, and the composition of Example 1 prepared above is shown together in Table 6 below.
- Example 1 Example 2
- Example 3 Example 4
- Example 5 Beef hydrolyzate (solid content 13.5 ⁇ 0.5%) 74 74.9 74.5 73.5
- Mushroom processed product (40% solid content)
- One 0.1 0.5 1.5 3
- Metal salt (salt, NaCl) 6 6 6 6 6 6
- Maltodextrin 11 11 11 11 11 additive 8 8 8 8 8 total 100 100 100 100 100 100 100 100 100
- FIG. 8 shows the results of component analysis for pyrazine-based compounds
- FIG. 9 shows the results of component analysis for fragrance components other than the pyrazine-based compounds
- FIG. 10 shows the results of component analysis for acetic acid.
- FIG. 11 shows the results of component analysis for glutamic acid and aspartic acid
- FIG. 12 shows the results of component analysis for amino acids other than glutamic acid and aspartic acid.
- the flavor enhancer prepared from Examples 1, 3 and 4 containing the mushroom processed product in an amount of 0.5% to 1.5% by weight in the composition for producing a flavor enhancer It was confirmed that the flavor enhancement effect by enhancing the flavor and reducing the taste and off-flavor is more excellently improved.
- Examples 1, 4 and 5 which showed a large difference in the content of pyrazine-based compounds, were selected to show the intensity of the meat flavor and the sensory and umami taste for the taste.
- the sensory evaluation was performed on the intensity of umami due to the difference in the content of acidic amino acids.
- Meat flavor intensity Meat flavor preferences Umami intensity Example 1 3.61 ab 3.39 ab 3.52 a Example 4 3.85 a 3.73 a 3.85 a Example 5 3.27 b 3.24 b 3.06 b
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Abstract
Description
성분 | 함량(g/L) |
아르기닌 | 1.97 |
글루코스 | 1.84 |
류신 | 1.77 |
말토스 | 1.69 |
페닐알라닌 | 1.49 |
수크로스 | 0.37 |
프럭토스 | 0.36 |
글루탐산 | 0.23 |
아스파르트산 | 0.02 |
기타 | 3.76 |
고형분 총합 | 13.5±0.5 |
성분 | 함량(%) |
메틸피라진 | 0.58 |
2,5-디메틸피라진 | 0.27 |
2,6- 디메틸피라진 | 0.74 |
2-에틸-6-메틸피라진 | 0.09 |
2,3,5-트리메틸피라진 | 0.15 |
2-아세틸퓨란 | 1.87 |
아세트산 | 4.41 |
디메틸 디설파이드 | 0.89 |
2,4,5-트리메틸옥사졸 | 0.00 |
2-펜틸 퓨란 | 0.16 |
2-아세틸 퓨란 | 1.87 |
성분(중량%) | 비교예 1 | 비교예 2 | 실시예 1 |
쇠고기 가수분해물(고형분 13.5 ±0.5 %) | 75 | 74.5 | 74 |
버섯 가공물(고형분 40 %) | - | - | 1 |
5'-이노신산이나트륨(IMP) | - | 0.5 | - |
금속염(소금, NaCl) | 6 | 6 | 6 |
말토덱스트린 | 11 | 11 | 11 |
첨가제 | 8 | 8 | 8 |
총합 | 100 | 100 | 100 |
온도 (℃) | 120℃ |
압력 (kgf/cm2) | 1.3 kgf/cm2 |
시간 (분) | 35분 |
GC-MS 조작 조건 | |
분석 기기 | 6890A gas chromatograph connected to a 5973N mass selective detector, Agilent Technologies |
자동 샘플러 | Multi-purpose sampler MPS, Herstel |
칼럼 | DB-WAX, 60m, 0.25 d, 0.25μm 필름 |
칼럼 충진제 | SPME fiber (50/30μm DVB/CAR/PDMS, stableflex 23Ga, auto sampler, 2cm) |
GC-MS 오븐 온도 | 40℃ (2분 유지) -> 3℃/분 -> 130℃ (유지 시간 없음) -> 3℃/분 -> 200℃ (6분 유지) |
동정 Library | W9N08, Wiley 9th edition and National Institute of Standards and Technology(NIST) version 08 |
RI(Retention Index) | n-alkane mixture을 외부표준물질로 사용하여 시료 분석 조건과 동일한 기기 조건 하에 분석하여 RT 값 계산 |
성분(중량%) | 실시예 1 | 실시예 2 | 실시예 3 | 실시예 4 | 실시예 5 |
쇠고기 가수분해물(고형분 13.5 ±0.5 %) | 74 | 74.9 | 74.5 | 73.5 | 72 |
버섯 가공물(고형분 40 %) | 1 | 0.1 | 0.5 | 1.5 | 3 |
금속염(소금, NaCl) | 6 | 6 | 6 | 6 | 6 |
말토덱스트린 | 11 | 11 | 11 | 11 | 11 |
첨가제 | 8 | 8 | 8 | 8 | 8 |
총합 | 100 | 100 | 100 | 100 | 100 |
고기 향미 강도 | 고기 향미 기호도 | 감칠맛 강도 | |
실시예 1 | 3.61ab | 3.39 ab | 3.52a |
실시예 4 | 3.85a | 3.73a | 3.85a |
실시예 5 | 3.27b | 3.24b | 3.06b |
Claims (13)
- 아미노산 및 당류를 포함하는 육류 가수분해물, 및 버섯 가공물을 포함하고,상기 육류 가수분해물의 고형분 100 중량부를 기준으로 상기 버섯 가공물의 고형분을 0.1 중량부 내지 20 중량부의 함량으로 포함하는 것인 향미증진제 제조용 조성물.
- 청구항 1에 있어서,상기 금속염을 더 포함하는 것인 향미증진제 제조용 조성물.
- 청구항 1에 있어서,상기 아미노산은 아르기닌, 류신, 페닐알라닌, 글루탐산, 아스파르트산 및 이의 염으로 이루어진 군으로부터 선택되는 적어도 하나를 포함하는 것인 향미증진제 제조용 조성물.
- 청구항 1에 있어서,상기 육류 가수분해물은 육류에 가수분해효소 처리하여 수득된 것인 향미증진제 제조용 조성물.
- 청구항 1에 있어서,상기 버섯 가공물은 버섯 추출물을 가열하여 제조한 것인 향미증진제 제조용 조성물.
- 청구항 1 내지 청구항 5 중 어느 한 항에 따른 향미증진제 제조용 조성물을 가열하는 단계를 포함하는 향미증진제의 제조 방법.
- 청구항 6에 있어서,상기 가열 전, 후 또는 동시에 상기 향미증진제 제조용 조성물을 농축하는 단계를 더 포함하는 것인 향미증진제의 제조 방법.
- 청구항 6 있어서,상기 가열은 100℃이상에서 수행하는 것인 향미증진제의 제조 방법.
- 피라진계 화합물 및 아세트산을 포함하는 향미증진제로서,상기 피라진계 화합물과 상기 아세트산의 함량비는 1:1.5 내지 1:2.5인 향미증진제.
- 청구항 9에 있어서,상기 피라진계 화합물 및 아세트산의 함량은 기체 크로마토그래피를 이용하여 측정한 것인 향미증진제.
- 청구항 9에 있어서,향미증진제 전체 아미노산 100 중량부 대비 14 중량부 이상 함량의 글루탐산 또는 이의 염을 포함하는 것인 향미증진제.
- 청구항 9에 있어서,상기 피라진계 화합물의 함량은 메틸피라진, 2,5-디메틸피라진, 2,6-디메틸피라진, 2-에틸-6-메틸피라진 및 2,3,5-트리메틸피라진의 함량의 총합을 나타내는 것인 향미증진제.
- 청구항 9에 있어서,상기 피라진계 화합물은 메틸피라진을 포함하고, 상기 피라진계 화합물과 상기 메틸피라진의 함량비는 1:0.20 내지 1:0.5인 향미증진제.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
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AU2020348144A AU2020348144B2 (en) | 2019-09-20 | 2020-09-18 | Flavour enhancer and method of preparing same |
US17/634,833 US20220295852A1 (en) | 2019-09-20 | 2020-09-18 | Flavour enhancer and method of preparing same |
EP20864400.5A EP4032412A4 (en) | 2019-09-20 | 2020-09-18 | FLAVOR ENHANCER AND METHOD FOR THE PRODUCTION THEREOF |
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CN114431448A (zh) * | 2022-02-23 | 2022-05-06 | 南京高新工大生物技术研究院有限公司 | 一种基于食用菌发酵技术的调味品及其制备方法 |
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CN115247137B (zh) * | 2021-04-28 | 2023-05-26 | 四川大学 | 一株能提升酱油风味的地衣芽孢杆菌及其在发酵食品中的应用 |
KR20230153169A (ko) | 2022-04-28 | 2023-11-06 | 농업회사법인(주)애니웰 | 동애등에 단백질 가수분해물을 이용한 반려동물 사료용 향미증진제의 제조방법 |
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KR102374566B1 (ko) | 2022-03-15 |
AU2020348144A1 (en) | 2022-03-24 |
KR20210034360A (ko) | 2021-03-30 |
AU2020348144B2 (en) | 2024-02-15 |
EP4032412A1 (en) | 2022-07-27 |
US20220295852A1 (en) | 2022-09-22 |
EP4032412A4 (en) | 2024-02-21 |
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