WO2021046959A1

WO2021046959A1 - Cleaning separation apparatus

Info

Publication number: WO2021046959A1
Application number: PCT/CN2019/111369
Authority: WO
Inventors: 吴国银; 郭金龙; 苗新利; 吴冬
Original assignee: 苏州长光华医生物医学工程有限公司
Priority date: 2019-09-11
Filing date: 2019-10-16
Publication date: 2021-03-18
Also published as: CN110586557B; CN110586557A

Abstract

A cleaning separation apparatus (100), comprising: a frame (102), a cleaning base (20), a cleaning rotating disc (40), a cleaning tower (60) disposed corresponding to the cleaning rotating disc (40), and a cleaning tower driving mechanism (80) controlled to drive the cleaning tower (60) to move, and a control device that controls cleaning. The washing rotating disc (40) is provided with a cup holder for containing a reaction cup or test tube. The cleaning base (20) is sequentially provided with a first combined cleaning station (202), a second combined cleaning station (204), and a third combined cleaning station (206). The first combined cleaning station (202) comprises a pre-magnetic position (2022) and a magnetic cleaning position (2024) that are arranged in sequence. The second combined cleaning station (204) or the third combined cleaning station (206) comprises a shaking position (2020), the pre-magnetic position (2022), and the magnetic cleaning position (2024) that are arranged in sequence. The cleaning base (20) is provided with a magnetic member (22) corresponding to the pre-magnetic position (2022) or the magnetic cleaning position (2024). According to the cleaning separation apparatus, magnetic particles are first adsorbed to the side wall of the reaction cup at the pre-magnetic position to prepare for the subsequent cleaning, magnetic beads are further tightly adsorbed to the side wall of the reaction cup at the magnetic cleaning position, thereby reducing the magnetic loss in the cleaning process.

Description

Cleaning and separating device

Technical field

The invention belongs to the field of medical equipment, and particularly relates to a cleaning and separating device suitable for chemiluminescence detection.

Background technique

Chemiluminescence immunoassay is: the use of biological immune technology to achieve the specific binding of the test substance, the luminescent label and the target substance, so that it emits light in an alkaline environment, and the released light energy corresponds to the concentration of the test substance monotonously. The concentration of the analyte can be obtained indirectly. The currently popular target substance is immunomagnetic particles, that is, ferrite particles coated with antibodies or antigens, which can be bound by a magnetic field to facilitate the separation of substances; luminescent markers are combined with chemiluminescent agents (acridine esters or isoluminol) The label is obtained on the antibody.

The basic process of chemiluminescence immunoassay is shown in Figure 1: 1. Add the sample, magnetic particles, and reagents to the reaction cup; 2. Incubate to fully combine the reactants; 3. Wash in a magnetic field to remove unbound substances; 4. Add excitation solution; 5. Perform signal detection. That is, the specific binding between the test substance, the label and the target substance is realized under constant temperature conditions to obtain immune complex particles (a combination of the three); then the immune complex is bound by a magnetic field, and the unbound antigen and the label are bound. Wash and remove; finally add hydrogen peroxide (substrate A) and sodium hydroxide (substrate B) solution (collectively called the excitation solution) to the immune complex, and make the isoluminol label transition to unstable high-energy excitation through the oxidation reaction When the atoms move back to the ground state, they emit a flash, and then the concentration information of the analyte in the sample can be obtained by measuring the light energy.

During the reaction process, the cleaning and separation mechanism or device needs to clean the substances that are not combined with the magnetic beads, otherwise it will cause abnormal test results.

Summary of the invention

Based on this, it is necessary to provide a cleaning and separating device that can reduce magnetic loss.

A cleaning separation device, comprising: a frame, a cleaning base set on the frame, a cleaning turntable set on the cleaning base, a cleaning tower set corresponding to the cleaning turntable, connected to the cleaning tower and controlled A cleaning tower driving mechanism that drives the movement of the cleaning tower, and a control device that controls and drives the cleaning turntable or the movement of the cleaning tower/or controls the cleaning. The cleaning turntable is provided with a cup holder for accommodating reaction cups or test tubes, and on the cleaning base Corresponding to the cup holder or the rotating ring where the cup holder is located and sequentially provided with a first combined cleaning station, a second combined cleaning station, and a third combined cleaning station, the first combined cleaning station includes: and The cup holder or the rotating ring on which the cup holder is located is correspondingly set and sequentially set with a pre-magnetic position and a magnetic cleaning position, the second combined cleaning station or the third combined cleaning station includes: and the cup holder or the cup holder The rotating ring is located correspondingly and sequentially set up a shaking position, a pre-magnetic position, and a magnetic cleaning position, and the cleaning base is provided with a magnetic part corresponding to the pre-magnetic position or the magnetic cleaning position.

In a preferred embodiment, the magnetic member includes: a magnetic pre-magnetic member arranged corresponding to the pre-magnetic position and placed on one side of the pre-magnetic position, and arranged corresponding to the magnetic cleaning position and placed opposite to the magnetic cleaning position. The magnetic first cleaning magnetic piece and the second cleaning magnetic piece on both sides of the position.

In a preferred embodiment, it further includes: a semi-annular or partial ring-shaped magnetic component loading tray for loading the pre-magnet and the first cleaning magnetic component, the pre-magnet and the first cleaning magnetic component are opposed to the The cup holder or the rotating ring where the cup holder is located is arranged on the inner ring, and the second cleaning magnetic member is disposed on the outer ring relative to the cup holder or the rotating ring where the cup holder is located.

In a preferred embodiment, the center of gravity of the first cleaning magnetic member and the second cleaning magnetic member arranged opposite to each other are set at the same height.

In a preferred embodiment, the center of gravity of the magnetic member corresponding to the third combined cleaning station is lower than the center of gravity of the magnetic member corresponding to the first combined cleaning station or the second combined cleaning station, and The height or the center of gravity of the magnetic member corresponding to the third combined cleaning station is set corresponding to the level of the excitation liquid or solution added to the reaction cup to be processed and is lower than the level of the liquid level.

In a preferred embodiment, the center of gravity of the pre-magnetic part corresponding to the pre-magnet position of the third combined cleaning station is lower than that of the magnetic part of the second combined cleaning station or the first combined cleaning station. -3mm setting.

In a preferred embodiment, the magnetic field strength of the pre-magnetic bit is 610-630 mT, and the magnetic field strength of the magnetic cleaning bit is 690-710 mT.

In a preferred embodiment, the shaking position includes: a shaking table corresponding to the rotating ring where the cup holder or the cup holder is located and arranged under or at the bottom of the rotating ring where the cup holder or the cup holder is located, A shaking and rotating drive device that drives the rotation of the shaking table and a shaking and lifting drive device that drives the up and down movement of the shaking table. The upper part of the shaking table is above the cup holder or the upper part of the rotating ring where the cup holder is located. Shaking baffles are arranged at the corresponding positions.

In a preferred embodiment, the shaking table is an eccentric wheel turntable, and the shaking baffle is set corresponding to the height of the reaction cup to be processed placed on the cup holder and is set to be 1-5 cm higher.

In a preferred embodiment, it further includes: a reset mechanism connected to the cleaning tower driving mechanism and driving it to drive the cleaning tower to enter the working position or reset; the cleaning tower driving mechanism includes: connected to the cleaning tower A cleaning tower lifting mechanism that drives the cleaning tower to perform a lifting movement, a lifting slide movably connected to the cleaning tower lifting mechanism and for the cleaning tower lifting mechanism to move along the lifting slide, connected to the cleaning tower lifting mechanism and driving it along the lifting slide A lifting driving power element for lifting movement, the resetting mechanism includes: a base plate arranged on the frame, a reset driving mechanism arranged on the base plate, and an arched or power-shaped cover reset arranged on the base plate Groove, a sliding block or sliding column connected to the cleaning tower lifting mechanism or lifting slide and moving along the reset groove, connected to the lifting slide and rotating and driving the lifting slide or lifting mechanism to pass along the sliding block or sliding column The resetting groove moves to reset the cleaning tower or drive it into the resetting driving part of the station; the cleaning tower is provided with a liquid extraction needle or a liquid extraction needle, which is used for liquid extraction during cleaning at the third combined cleaning station The pipe diameter or the pumping speed of the pipeline is lower than the pumping pipeline or the pumping speed when the first combined cleaning station or the second combined cleaning station performs cleaning.

The above cleaning and separation device first adsorbs the magnetic particles to the side wall of the reaction cup through the pre-magnetic position to prepare for the subsequent cleaning. The magnetic cleaning position further tightly adsorbs the magnetic beads on the side wall of the reaction cup, reducing the cleaning process The magnetic loss.

In addition, the magnetic parts of the magnetic cleaning position are arranged in pairs, the center of gravity is flush, which improves the magnetic field strength and reduces the magnetic loss.

Furthermore, the magnetic beads are thoroughly shaken and dispersed before cleaning to avoid abnormal luminescence values caused by improper cleaning of the magnetic beads wrapped in acridinium.

Further, the center of gravity of the magnet in the third combined cleaning station is moved down to prevent the height of a small number of magnetic beads from exceeding the height of the subsequent injection substrate, resulting in magnetic loss.

Furthermore, the pipeline of the magnetic cleaning station of the third combined cleaning station is thinner, which reduces the waste liquid extraction speed and avoids the magnetic loss caused by the pull-down of the magnetic beads.

Description of the drawings

Figure 1 is a schematic diagram of the basic process of chemiluminescence immunoassay in the background art;

2 is a schematic diagram of a part of the structure of a cleaning and separating device according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of a part of the structure of a cleaning station and a magnetic member according to an embodiment of the present invention.

detailed description

As shown in FIGS. 2 to 3, the cleaning and separating device 100 according to an embodiment of the present invention includes: a frame 102, a cleaning base 20 set on the frame 102, a cleaning turntable 40 set on the cleaning base 20, and a cleaning turntable 40, a cleaning tower 60 corresponding to the cleaning tower 60, a cleaning tower drive mechanism 80 that is connected to the cleaning tower 60 and controlled to drive the cleaning tower 60 to move, and a control device that controls and drives the cleaning turntable 40 or the cleaning tower 60 to move/or controls the cleaning.

The washing turntable 40 of this embodiment is provided with a cup holder for accommodating reaction cups or test tubes. The cleaning base 20 of this embodiment is set corresponding to the cup holder or the rotating ring where the cup holder is located, and is provided with a first combined cleaning station 202, a second combined cleaning station 204, and a third combined cleaning station 206 in sequence.

The first combined cleaning station 202 includes: a pre-magnetic position 2022 and a magnetic cleaning position 2024 that are set corresponding to the cup holder or the rotating ring where the cup holder is located and sequentially set.

The second combined cleaning station 204 or the third combined cleaning station 206 includes: a shaking position 2020, a pre-magnetic position 2022, and a magnetic cleaning position 2024 that are set corresponding to the rotating ring where the cup holder or the cup holder is located and sequentially set.

The cleaning base 20 of this embodiment is provided with a magnetic member 22 corresponding to the pre-magnetic position 2022 or the magnetic cleaning position 2024. In order to facilitate installation and to ensure the stability of the provided magnetic field strength to a certain extent, it is preferable that the magnetic member 22 in this embodiment adopts a magnet.

The magnetic member 22 of this embodiment includes: a magnetic pre-magnetic member 222 arranged corresponding to the pre-magnetic position 2022 and placed on one side of the pre-magnetic position 2022, and two magnetic pre-magnetic members 222 arranged corresponding to the magnetic cleaning position 2024 and positioned opposite to the magnetic cleaning position 2024 The magnetic first cleaning magnetic member 224 and the second cleaning magnetic member 226 on the side.

Further, the cleaning and separating device 100 of this embodiment further includes: a semi-annular or partial-annular magnetic component loading tray 200 arranged in the cleaning base 20 and loaded with the pre-magnet 222 and the first cleaning magnetic component 224.

Preferably, in order to facilitate installation and loading, the pre-magnetic member 222 and the first cleaning magnetic member 224 of this embodiment are arranged in the inner ring relative to the cup holder or the rotating ring where the cup holder is located. The second cleaning magnetic member 226 is arranged on the outer ring relative to the cup holder or the rotating ring where the cup holder is located.

Furthermore, the outer wall of the cleaning base 20 of this embodiment is further provided with a heat preservation layer 27. The thermal insulation layer 27 of this embodiment is covered with thermal insulation cotton to clean the outer wall of the base 20.

The rack 102 in this embodiment includes a base 104 and a support frame 106 arranged on the base 104. The cleaning base 20 of this embodiment is arranged on the support frame 106 and is supported and fixed by the support frame 106.

In order to ensure the symmetry of the magnetic field and the strength of the magnetic field when the cuvette is cleaned, the center of gravity of the first cleaning magnetic member 224 and the second cleaning magnetic member 226 arranged opposite to each other in this embodiment are set at the same height.

Further, in order to pull down the magnetic beads in the reaction cup on the third combined cleaning station to reduce magnetic loss, the center of gravity of the magnetic part corresponding to the third combined cleaning station 206 in this embodiment is lower than that of the first combined cleaning station. The center of gravity of the magnetic component corresponding to the station 202 or the second combined cleaning station 204 is set.

Further, in order to avoid the excitation liquid injected into the reaction cup 33, such as the injected substrate A (hydrogen peroxide), not immersing all the magnetic beads and causing magnetic loss, in this embodiment, the height of the magnetic member set corresponding to the third combined cleaning station 206 Or the center of gravity is set corresponding to the level of the excitation liquid or solution added to the reaction cup 33 to be processed and is lower than the level of the liquid level.

When the first combined cleaning station 202 or the second combined cleaning station 204 performs cleaning, in order to completely adsorb the magnetic beads in the reaction cup, avoid the failure of the magnetic beads placed on the upper part due to the height of the reaction solution during the previous reaction. The magnetic parts such as magnets of the second combined cleaning station or the first combined cleaning station of this embodiment are relatively set to be relatively high, so as to prevent the magnetic beads that are not adsorbed or have insufficient adsorption force from being Pull out. After adsorption, in the later stage of cleaning and separation, gradually adjust the height position of the magnetic member to reduce the height of the magnetic member or the height of the center of gravity. In this embodiment, the pre-magnetic member set corresponding to the pre-magnetic position of the third combined cleaning station 206 The center of gravity is set 1-3 mm lower than the magnetic part of the second combined cleaning station 204 or the first combined cleaning station 202 by 1-3 mm.

In this embodiment, the magnetic beads are initially adsorbed through the pre-magnetic position 2022, and then further adsorbed on the magnetic cleaning position to completely adsorb all the magnetic beads as much as possible to prevent the magnetic beads from escaping and causing magnetic loss. The magnetic field strength of the pre-magnetic bit in this embodiment is 610-630 mT, and the magnetic field strength of the magnetic cleaning bit is 690-710 mT. Preferably, the magnetic field strength of the pre-magnetic bit in this embodiment is 620 mT, and the magnetic field strength of the magnetic cleaning bit is 700 mT. It not only avoids magnetic loss, but also prevents excessive magnetic field strength from affecting other components or operations.

The shaking position 2020 of this embodiment is provided with: a shaking table 228 which is arranged corresponding to the rotating ring where the cup holder or the cup holder is located and is arranged under or at the bottom of the rotating ring where the cup holder or the cup holder is located, and the shaking table 228 is driven to perform A shaking and rotating drive device for eccentric rotation and a shaking and lifting drive device for driving the up and down movement of the shaking table 228.

In order to avoid liquid splashing during shaking, the shaking table 228 of this embodiment is provided with a shaking baffle 229 at the corresponding position of the cup holder or the upper part of the rotating ring where the cup holder is located.

Further, the shaking table 228 of this embodiment is an eccentric turntable. The shaking baffle 229 is set corresponding to the height of the reaction cup to be processed placed on the cup holder and is set to be 1-5 cm higher. Preferably, the setting height of the shaking baffle 229 is set 2 cm higher than the height of the reaction cup to be processed placed on the cup holder. In this way, the reaction cup and the shaker baffle 229 are prevented from contacting each other and rubbed against each other during shaking; at the same time, the shaking table 228 is driven to move up by the shaker lifting drive device to squeeze and slightly lift the reaction cup to fully meet the reaction cup. Contact, to avoid incomplete or insufficient shaking caused by insufficient contact with the reaction cup, and at the same time avoid the collision or collision between the reaction cup and the shaking baffle 229 after the reaction cup is raised, causing friction.

Further, the cleaning and separation device 100 of this embodiment further includes a reset mechanism 90 connected to the cleaning tower drive mechanism 80 and driven to drive the cleaning tower 60 into the working position or reset.

The cleaning turntable 40 of this embodiment may adopt an indexing disk structure, and the rotation angle of the cleaning turntable 40 can be adjusted by controlling and adjusting the angular position of the indexing disk. The shape of the cleaning tower 60 in this embodiment is set corresponding to the cleaning turntable 40, and is set in a semi-annular or partial ring.

Further, the cleaning tower driving mechanism 80 of this embodiment includes: a cleaning tower lifting mechanism 82 connected to the cleaning tower 60 to drive the cleaning tower 60 to perform lifting movement, and movably connected to the cleaning tower lifting mechanism 82 for the cleaning tower lifting mechanism 82 to move along it. The moving lifting slide rail 84 is connected to the cleaning tower lifting mechanism 82 and driving it to perform lifting motion along the lifting slide 84.

The lifting driving power element of this embodiment can be driven by a hydraulic system or a pneumatic system for lifting linear motion, or a motor (a reducer can be added as needed) is converted into a linear motion to drive the cleaning tower lifting mechanism 82 for lifting motion.

The reset mechanism 90 of this embodiment includes: a substrate 92 provided on the frame 102, a reset driving mechanism 94 provided on the substrate 92, an arched or power-shaped cover-shaped reset groove 94 provided on the substrate 92, and a cleaning The tower lifting mechanism 82 or the lifting slide rail 84 is connected and arranged in the reset groove to move along the reset groove slide or sliding column 96, connected to the lifting slide 84 and driven by the rotation drive to drive the lifting slide 84 or the cleaning tower lifting mechanism 82 to pass The sliding block or spool 96 moves along the reset groove 94 to reset the cleaning tower 60 or drive it into the reset drive 98 of the station, and the reset power element that drives the reset drive 98 to move. The reset power element can be a motor, and a reducer can be added as needed. Power components that can provide power such as ordinary motors, stepping motors, or servo motors can be used.

Furthermore, the cleaning tower 60 of this embodiment is provided with a liquid extraction needle or a liquid extraction needle 30. In this embodiment, the pipe diameter or the pumping speed of the liquid suction pipeline during cleaning in the third combined cleaning station 206 is lower than that of the first combined cleaning station 202 or the second combined cleaning station 204 when cleaning. Pipeline or pumping speed.

In this embodiment, each reaction cup is cleaned in five stages, the first and second stages are cleaned in the first combined cleaning station 202, the third and fourth stages are cleaned in the second combined cleaning station 204, and the fifth stage is cleaned. It is carried out at the third combined cleaning station 206.

The control device of this embodiment controls and drives the cleaning turntable 40 to rotate, and drives the reaction cup 33 in the cup holder set on it to enter the first combined cleaning station 202, and first passes through the pre-magnetic position 2022 of the first combined cleaning station 202, The pre-magnetic part 222 of the pre-magnetic position 2022, such as a magnet, is arranged on the inner ring of the cup holder or the rotating ring where the cup holder is located. The magnetic field strength is preferably 620mT, or more than 620mT, and the magnetic beads in the reaction cup are gathered in the cleaning turntable 40. The inner and outer rings of the magnetic cleaning station 2024 of the first combined cleaning station 202 are equipped with magnetic parts, that is, the first cleaning magnetic part 224 and the second cleaning magnetic part 226. The first cleaning magnetic part 224 can be one or more One or more second cleaning magnetic members 226 can also be provided. In this embodiment, in order to ensure the consistency of the center of gravity and the stability of the magnetic field, in this embodiment, the first cleaning magnetic part 224 adopts a monolithic magnet for realization, and the second cleaning magnetic part 226 also adopts a monolithic structure. The magnet is implemented. The first cleaning magnetic part 224 and the second cleaning magnetic part 226 are arranged in the inner and outer rings relative to the magnetic cleaning position 2024 of the first combined cleaning station 202, and the center of gravity is equal, and the magnetic field strength is further strengthened, reaching about 700mT. This position is cleaned twice. When cleaning, the peristaltic pump tubing connected to the waste pumping needle uses a 2.9-3.6mm inner diameter tubing, and the waste pumping speed is about 0.9-1.1 mL/s. Preferably, in this embodiment, when cleaning is performed at the magnetic cleaning position 2024 of the first combined cleaning station 202, the peristaltic pump pipeline connected to the waste liquid extraction needle uses a 3.2 mm inner diameter pipeline, and the waste liquid extraction speed is about 1 mL /s. After the two-stage cleaning is performed at the magnetic cleaning position 2024 of the first combined cleaning station 202, the reaction cup 33 is rotated to the shaking position 2020 to fully disperse the magnetic beads, and then the acridinium ester and the like wrapped in the middle of the magnetic beads are not involved. The reacted substances are fully released. After shaking, the reaction cup 33 continues to rotate to the pre-magnetic position 2022 of the second combined cleaning station 204, and the magnetic cleaning position 2024. The magnetic parts at these two positions are such as the magnet configuration and the pre-magnetized position of the first combined cleaning station 202. The magnetic parts of the position 2022 or the magnetic cleaning position 2024 have the same configuration as the magnet. When the magnetic cleaning position 2024 of the second combined cleaning station 204 is cleaned, the inner diameter of the waste liquid pipe and the liquid suction speed are also set to be the same.

After being cleaned by the magnetic cleaning station 2024 of the second combined cleaning station 204, the reaction cup 33 enters the shaking again. Enter the pre-magnetic position 2022 of the third combined cleaning station 206 to pre-magnetize. At this time, the center of gravity of the pre-magnet 222 is higher than the pre-magnetic position 2022 or magnetic cleaning position of the first combined cleaning station 202 or the second combined cleaning station 204 The magnetic part of 202 4 is about 1-3 mm lower, preferably about 2 mm, to achieve pre-pulling of the magnetic beads in the reaction cup.

Then enter the magnetic cleaning position 2024 of the third combined cleaning station 206. The magnetic parts of the inner and outer rings of this position, namely the first cleaning magnetic part 224 and the second cleaning magnetic part 226, are respectively arranged on the cup holder or the rotating ring where the cup holder is located. The center of gravity of the inner and outer circles is further reduced.

The first cleaning magnetic part 224 and the second cleaning magnetic part 226 of the third combined cleaning station 206 are set according to the volume of the excitation liquid added, and their center of gravity is lower than the liquid level of the solution in the reaction cup 33, such as 275 μl in the reaction cup Liquid, in order to avoid the accumulation of a small amount of magnetic beads exceeding the height of the liquid level of 275μl, the magnetic beads are not overwhelmed or covered when the substrate A is injected, resulting in magnetic loss, the first cleaning of the third combined cleaning station 206 of this embodiment The center of gravity of the magnetic member 224 and the second cleaning magnetic member 226 can be set at a distance of about 2-3.5 mm from the bottom of the reaction cup, preferably about 2.5-3 mm. Here, after cleaning, the inner diameter of the waste liquid pipeline is 1.5-2.5mm, and the flow rate of the waste liquid is about 0.7-0.9 mL/s. Preferably, the inner diameter of the waste liquid extraction pipeline is 2.0 mm, and the flow rate of the waste liquid extraction is about 0.8 mL/s. On the one hand, pull down the magnetic beads to prevent some magnetic beads from being distributed on the upper part of the reaction cup and cause magnetic loss when the substrate is subsequently injected. At the same time, the waste liquid extraction speed here is also reduced to reduce the magnetic loss.

In the process of chemiluminescence test, the sample, reagent and magnetic particles are combined after incubation and fully reacted, the unbound substances need to be removed in the magnetic field, and then excitation liquid (substrate A, B) is added to make it glow, and then the test is obtained. result. During the entire reaction process, the cleaning and separation device needs to clean the substances that are not bound to the magnetic beads, otherwise it will cause abnormal test results. However, the magnetic field strength of the existing magnet arrangement method is low, and the position of the magnet's center of gravity is unreasonably arranged, which is prone to magnetic loss. In the present invention, each reaction cup is cleaned in five stages, and the first four stages are cleaned. Before cleaning, the reaction cup goes through the shaking position 2020, the pre-magnetic position 2022, and the magnetic cleaning position 2024 (each magnetic cleaning position is cleaned twice), and the pre-magnetic position is 2022. Compared with the inner ring of the cleaning turntable 40, a magnet of 620mT or more is arranged to gather the magnetic beads. The magnetic cleaning position 2024 is equipped with magnetic parts such as magnets. Through the control of tooling, the end face is as close to the reaction cup as possible, and the center of gravity is aligned, and the magnetic field strength can be adjusted. Increase to 700mT. The configuration of the fifth stage cleaning magnet is the same as that of the previous four stages, but the center of gravity of the magnet is lowered to about 2.5-3mm from the bottom of the reaction cup. Being overwhelmed, magnetic loss occurs.

In addition, the diameters of the waste liquid pipes of the magnetic cleaning station 2024 of the first combined cleaning station 202, the second combined cleaning station 204, and the third combined cleaning station 206 are respectively 3.2mm, 3.2mm, 2.0mm, and the third The pipeline of the magnetic cleaning station 2024 of the combined cleaning station 206 is thinner, which reduces the waste liquid extraction speed and avoids magnetic loss due to the pull-down of the magnetic beads.

The cleaning and separating device of the present invention is applied to a chemiluminescence magnetic separation cleaning unit, and the magnetic cleaning position 2024 magnets are arranged in pairs, the center of gravity is flush, the magnetic field strength is improved, and the magnetic loss is reduced. Before cleaning, the magnetic beads are thoroughly shaken to disperse, to avoid the abnormal luminescence value caused by the unclean cleaning of the magnetic beads wrapped acridinium. The magnet's center of gravity of the third combined cleaning station 206 moves down to prevent the height of a small number of magnetic beads from exceeding the height of the subsequent injection substrate, resulting in magnetic loss.

The present invention adopts five-stage cleaning. The first and second-stage cleaning are at the magnetic cleaning station 2024 of the first combined cleaning station 202, and the third and fourth-stage cleaning are at the magnetic cleaning station 2024 of the second combined cleaning station 204. The fifth stage The cleaning is performed at the magnetic cleaning position 2024 of the third combined cleaning station 206, and the reaction cup is shaken, pre-magnetized, and cleaned in sequence before entering each stage of cleaning. Shaking well can fully disperse the magnetic beads in the reaction cup, and fully release the unbound substances such as acridinium esters wrapped in the magnetic beads; the pre-magnetic position adsorbs the magnetic particles to the side wall of the reaction cup first, which is the next step. Make preparations for magnetization during cleaning; the magnetic field strength of the magnetic cleaning position 2024 is further strengthened, and the magnetic beads are tightly adsorbed on the side wall of the reaction cup to reduce the magnetic loss during the cleaning process.

The above-mentioned embodiments only express several implementation modes of the present invention, and the description is relatively specific and detailed, but it should not be understood as a limitation to the patent scope of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can be made, and these all fall within the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention should be subject to the appended claims.

Summary of the invention

technical problem

The solution to the problem

The beneficial effects of the invention

Claims

A cleaning and separating device, which is characterized by comprising: a frame, a cleaning base set on the frame, a cleaning turntable set on the cleaning base, a cleaning tower set corresponding to the cleaning turntable, and the cleaning tower The cleaning tower drive mechanism connected and controlled to drive the cleaning tower movement, the cleaning turntable or the cleaning tower movement/or the control device for controlling the cleaning, the cleaning turntable is provided with a cup holder containing reaction cups or test tubes, so The cleaning base is set corresponding to the rotating ring where the cup holder or the cup holder is located, and is sequentially provided with a first combined cleaning station, a second combined cleaning station, and a third combined cleaning station. The first combined cleaning station is The positions include: a pre-magnetic position and a magnetic cleaning position that are set correspondingly to the cup holder or the rotating ring where the cup holder is located, and the second combined cleaning station or the third combined cleaning station includes: and the cup holder The rotating ring on which the rack or the cup holder is located is correspondingly arranged and sequentially set up a shaking position, a pre-magnetic position, and a magnetic cleaning position, and the cleaning base is provided with a magnetic part corresponding to the pre-magnetic position or the magnetic cleaning position.
The cleaning and separating device according to claim 1, wherein the magnetic member comprises: a magnetic pre-magnetic member arranged corresponding to the pre-magnetic bit and placed on one side of the pre-magnetic bit, and the magnetic cleaning bit The first magnetic cleaning magnetic piece and the second magnetic cleaning magnetic piece are correspondingly arranged and placed opposite to the two sides of the magnetic cleaning position.
The cleaning and separating device according to claim 2, further comprising: a semi-annular or partial ring-shaped magnetic member loading disk for loading the pre-magnetic member, the first cleaning magnetic member, the pre-magnetic member, The first cleaning magnetic piece is arranged on the inner ring relative to the cup holder or the rotating ring where the cup holder is located, and the second cleaning magnetic piece is arranged on the outer ring relative to the cup holder or the rotating ring where the cup holder is located.
The cleaning and separating device according to claim 2, wherein the center of gravity of the first cleaning magnetic part and the second cleaning magnetic part that are arranged opposite to each other are arranged at the same height.
The cleaning and separating device according to any one of claims 2 to 4, wherein the center of gravity of the magnetic part corresponding to the third combined cleaning station is lower than that of the first combined cleaning station or the second combined cleaning station. The center of gravity of the magnetic member corresponding to the combined cleaning station, the height or center of gravity of the magnetic member corresponding to the third combined cleaning station is set corresponding to the level of the excitation liquid or solution added to the reaction cup to be processed and is lower than The height of its liquid level.
The cleaning and separating device according to claim 5, wherein the center of gravity of the pre-magnet member corresponding to the pre-magnet position of the third combined cleaning station is opposite to that of the second combined cleaning station or the first combined cleaning station. The magnetic part of the cleaning station is set 1-3mm lower.
The cleaning and separating device according to any one of claims 1 to 4, wherein the magnetic field strength of the pre-magnetic bit is 610-630 mT, and the magnetic field strength of the magnetic cleaning bit is 690-710 mT.
The cleaning and separating device according to any one of claims 1 to 4, wherein the shaking position comprises: corresponding to the cup holder or the rotating ring where the cup holder is located and arranged on the cup holder or the cup holder. The shaking table below or at the bottom of the rotating ring where the rack is located, the shaking rotation driving device that drives the shaking table to rotate, and the shaking lifting driving device that drives the lifting motion of the shaking table, above the shaking table Shaking baffles are arranged at corresponding positions on the upper part of the rotating ring where the cup holder or the cup holder is located.
The cleaning and separating device according to claim 8, wherein the shaking table is an eccentric wheel turntable, and the shaking baffle is set correspondingly to the height of the reaction cup to be processed placed on the cup holder and is higher 1-5cm setting.
The cleaning and separating device according to any one of claims 1 to 4, further comprising: a resetting mechanism connected to the cleaning tower driving mechanism and driving it to drive the cleaning tower into the working position or resetting, the The cleaning tower drive mechanism includes: a cleaning tower lifting mechanism connected to the cleaning tower to drive the cleaning tower to perform lifting motion, a lifting slide movably connected to the cleaning tower lifting mechanism and for the cleaning tower lifting mechanism to move along it, and The cleaning tower lifting mechanism is connected to and drives the lifting drive power element for lifting movement along the lifting slide rail. The resetting mechanism includes: a base plate arranged on the frame, and a resetting drive mechanism arranged on the base plate. The arch-shaped or power-shaped cover-shaped reset groove on the base plate, the sliding block or the sliding column connected with the cleaning tower lifting mechanism or the lifting slide rail and moving along the reset groove, connected with the lifting slide rail and driven by rotation The lifting slide or the lifting mechanism moves along the reset groove through the sliding block or the sliding column to reset the cleaning tower or drive it into the reset driving part of the station; the cleaning tower is provided with a liquid extraction needle or a liquid extraction needle, The pipe diameter or the pumping speed of the liquid suction pipeline when the third combined cleaning station performs cleaning is lower than the liquid suction pipeline or the liquid suction speed when the first combined cleaning station or the second combined cleaning station performs cleaning .