WO2021033213A1 - Mécanisme d'étalonnage de volume d'admission de pipette, pipette et procédé d'étalonnage de celui-ci - Google Patents

Mécanisme d'étalonnage de volume d'admission de pipette, pipette et procédé d'étalonnage de celui-ci Download PDF

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Publication number
WO2021033213A1
WO2021033213A1 PCT/JP2019/032126 JP2019032126W WO2021033213A1 WO 2021033213 A1 WO2021033213 A1 WO 2021033213A1 JP 2019032126 W JP2019032126 W JP 2019032126W WO 2021033213 A1 WO2021033213 A1 WO 2021033213A1
Authority
WO
WIPO (PCT)
Prior art keywords
screw
suction capacity
housing
pipette
stroke
Prior art date
Application number
PCT/JP2019/032126
Other languages
English (en)
Japanese (ja)
Inventor
俊一 森本
佐々木　潤
Original Assignee
株式会社 ニチリョー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社 ニチリョー filed Critical 株式会社 ニチリョー
Priority to CN201980099267.1A priority Critical patent/CN114222629B/zh
Priority to PCT/JP2019/032126 priority patent/WO2021033213A1/fr
Priority to JP2020513940A priority patent/JP6788311B1/ja
Publication of WO2021033213A1 publication Critical patent/WO2021033213A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
    • G01F11/00—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it
    • G01F11/02—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with measuring chambers which expand or contract during measurement
    • G01F11/04—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with measuring chambers which expand or contract during measurement of the free-piston type
    • G01F11/06—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with measuring chambers which expand or contract during measurement of the free-piston type with provision for varying the stroke of the piston

Definitions

  • the present invention relates to a mechanism for calibrating the suction capacity of a pipette, a pipette, and a method thereof.
  • the amount of liquid to be sucked into the pipette is variably set in advance.
  • the stroke screw which is relatively movable with respect to the vertically movable central shaft of the pipette and is screwed into the housing or the like, is manually rotated in the axial direction to adjust the movement.
  • the movement adjustment at this time is performed until the numerical display of the capacitance display mechanism driven in conjunction with the rotation of the stroke screw changes from, for example, 100 ⁇ l to 200 ⁇ l, which is a desired set amount.
  • the capacity setting sleeve 10 is pressed upward via the torque plate 26 by the torque spring 25 housed in the capacity display mechanism housing 14b, and therefore, the lock ring 27 and the lock ring in which the collar portion 10d is fixed to the housing 1 It is in contact with the washer 28. As a result, the capacity setting sleeve 10 is applied with torque in the rotation direction and does not generate unnecessary free rotation.
  • Reference numeral 11 denotes an ejector button, which is connected to the ejector pipe 13 via an ejector connection sleeve 12 (fitted to the lower end of the housing so as to be movable in the axial direction).
  • ejector members 11, 12 and 13 can integrally move downward against the ejector spring 41 at the time of ejecting the tip 43 (see FIG. 6) to eject the tip 43.
  • 21 is a lower two-stage spring receiver
  • 22 is an upper two-stage spring receiver
  • the step spring 23 urges the upper two-step spring receiver 22 upward via its flange portion 22a to bring it into contact with the stop ring 24.
  • Reference numeral 51 denotes a jig for calibration work, which has a grip portion 51a, a bent portion 51b, and a pair of engaging claw portions 51c.
  • the tip 43 is immersed in the liquid with the push button 29 pushed downward (to the right in FIG. 6) against the one-step spring 18 together with the shaft 2 and the plunger 3. Subsequently, when the pushing force is released, the members 29, 2 and 3 return to the upper motion and return to the state of FIG. 6, and at this time, a predetermined amount of liquid is sucked into the tip 43 and the lower nozzle housing 4.
  • the stroke screw 7 moves up and down in the axial direction based on the screw screwing to the fixed system housing 1 and the like (in this case, the capacity display mechanism housing 14a) during the above rotation.
  • the adjusting screw 9 since the adjusting screw 9 is integrally rotating with the stroke screw 7 based on the screw friction, it moves up and down together with the stroke screw 7.
  • the shaft 2 in contact with the lower end contact portion 9d of the adjusting screw 9 moves up and down, so that the plunger 3 also moves up and down, and the actual suction capacity corresponds to the change in the above displayed value. It changes from 90 ⁇ l to 100 ⁇ l.
  • the stroke screw 7 rotates integrally with the setting sleeve 10 to form the housing. It moves relative to the 1st class and the adjusting screw 9 by screwing each. At this time, since the adjusting screw 9 is restrained from rotating by the jig 51 and does not move in the axial direction as described above, only the stroke screw 7 is eventually moved with respect to the fixed system housing 1 and the like.
  • the rotating drum 15 of the capacity display mechanism 8 is interlocked by the rotation of the stroke screw 7. Driven by. Then, the setting sleeve 10 is manually rotated until the display value of the capacitance display mechanism 8 reaches 105 ⁇ l. As a result, 100 ⁇ l of the displayed value is changed to 105 ⁇ l, which is the same as the actual suction capacity, and the calibration work is completed.
  • the calibration work jig 51 is removed, and the push button 29 is reattached to the shaft 2.
  • the work of attaching and detaching the set screw 30 in the present embodiment is only for the purpose of securing a working space for the jig 51, and it is possible to eliminate this work, according to the first conventional example. It does not constitute an essential part of the invention as in the work of attaching and detaching the set screw 13.
  • the calibration pipe 4 had to be kept separated from the clutch pipe 3 against the spring throughout the period of the calibration work. Since it is sufficient to suppress the movement of the adjusting screw 9 in the rotational direction by the tool 51, the burden on the operator is reduced.
  • the screw pitch of the fine adjustment screw 12 (for example, 0) is larger than the screw pitch of the stroke screw (hollow shaft) 9 (for example, two types of 0.64 mm and 0.8 mm). Since .5 mm) was smaller, the conversion table was used to convert how many rotations of the stroke screw 9 (which directly corresponds to the capacitance value) corresponds to one rotation of the fine adjustment screw 12.
  • the screw pitches of the stroke screw 7 and the adjusting screw 9 are substantially the same, one rotation of the adjusting screw 9 directly corresponds to one rotation of the stroke screw 7, so that the capacitance is calibrated. It is convenient because it can be intuitively known from the display value of the capacity display mechanism 8.
  • FIG. 17 shows another jig 52 as an alternative.
  • the jig 52 has an L-shaped arm portion 52a (having a pair of engaging claw portions 52b, but only one in FIG. 16 is shown) protruding downward from the side of the jig main body and an engaging recess in the lower part of the main body. It has 52c.
  • the pair of engaging claws 52b of the jig 52 are engaged with the engaging recess 9b of the adjusting screw 9, and the engaging recess 52c at the bottom of the main body is integrated with the grip portion 1a of the housing 1 (integrated with the housing 1). If it is, other parts may be used).
  • the adjusting screw 9 is restrained and fixed so as to be relatively non-rotatable with respect to the housing 1, and it is not necessary for the operator to keep holding the jig during the calibration work period, which reduces the labor.
  • any kind of jig other than the jig 52 can be used as long as the adjusting screw 9 and the housing 1 can be detachably connected to each other to suppress the rotation of the adjusting screw 9.
  • a pipette in which a shaft (2) and a plunger (3) reciprocate in the axial direction in a housing (1) to suck and discharge a liquid into a nozzle housing (4).
  • a stroke screw that is screwed between the body (1) and the shaft (2) to the inner circumference of the housing (1) or the like and is fitted so as to be movable in the axial direction relative to the shaft (2).
  • a capacity display mechanism (8) driven in conjunction with the rotation of the stroke screw (7) and capable of variably displaying the suction capacity value, and the shaft screwed into the stroke screw (7).
  • a suction capacity variable setting screw member (9) that determines the stop position in the return direction of (2).
  • the third form is the first form or the second form, and preferably, the capacity display mechanism (8) has a capacity display rotating drum (15) housed in the housing (14), and the suction capacity adjustment.
  • the screw member (9) is urged in the upward return direction of the shaft (2) by a torque spring (25) interposed between the suction capacity adjusting screw member (9) and the housing (14). ..
  • the suction capacity variable setting screw member (9) is provided with the first engaging portion (9b), which is separate when the suction capacity calibration is performed.
  • the suction capacity variable setting screw By engaging the second engaging portion (51c, 52b) of the calibration work jig (51, 52) provided in the above with the first engaging portion (9b), the suction capacity variable setting screw The non-rotatable suppression of the member (9) can be realized.
  • the calibration work jig (52) further has a third engaging portion (52c), and the second engaging of the jig (52).
  • the portion (52b) engages with the first engaging portion (9b) of the suction capacity variable setting screw member (9), and the third engaging portion (52c) becomes a part of the housing (1). By engaging, it is possible to suppress the non-rotation of the suction capacity variable setting screw member (9).
  • the sixth form is more preferably a screw pitch screwed into the inner circumference of the housing (1) of the stroke screw (7) and the suction capacity variable setting.
  • the screw pitch of the screw member (9) screwed into the stroke screw (7) is substantially the same.
  • the seventh form is a pipette having the mechanism described in any of the first to sixth forms.
  • the shaft (2) and the plunger (3) reciprocate in the axial direction in the housing (1) to suck and discharge the liquid into the nozzle housing (4).
  • the shaft (2) is screwed directly or indirectly to the inner circumference of the housing (1) and fitted to the shaft (2) so as to be integrally rotatable and separately movable in the axial direction.
  • the pipette further including a suction capacity variable setting screw member (9) which is screwed and determines a stop position in the return direction of the shaft (2).
  • the stroke is further provided with a setting member (10) integrally rotatably fitted to the stroke screw (7), and the stroke is manually rotated by manually rotating the setting member (10).
  • a setting member 10 integrally rotatably fitted to the stroke screw (7)
  • the stroke is manually rotated by manually rotating the setting member (10).
  • the capacity display mechanism (8) includes a capacity display rotating drum (15) housed in a housing (14), and the suction capacity adjusting screw member (9). ) Is the method described above, wherein the shaft (2) is urged in the returning direction by a torque spring (25) interposed between the suction capacity adjusting screw member (9) and the housing (14).
  • the suction capacity variable setting screw member (9) is provided with the first engaging portion (9b), which is separate when the suction capacity calibration is performed.
  • the suction capacity variable setting screw member ( 9) This is the method that can realize the non-rotatable suppression.
  • the fixing jig (52) further has a third engaging portion (52c), and the second engaging portion (52b) of the jig (52) is said.
  • the thirteenth form includes a screw pitch screwed into the inner circumference of the housing (1) of the stroke screw (7) and the suction capacity variable setting screw member (9). ) Is substantially the same as the screw pitch screwed into the stroke screw (7).

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  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

L'invention concerne un mécanisme d'étalonnage d'un volume d'admission de pipette, une pipette, et un procédé d'étalonnage de celui-ci, qui rendent facile l'étalonnage du volume d'admission d'une pipette, avec un mécanisme simple.　Dans une pipette pour amener le liquide à entrer/sortir de l'intérieur d'un boîtier de buse (4) avec un mouvement de va-et-vient d'un arbre (2) et un plongeur (3) dans la direction axiale à travers l'intérieur d'une enceinte (1), ce mécanisme d'étalonnage d'un volume d'admission de pipette étant en outre pourvu : d'une vis à course (7) qui est filetée sur une circonférence interne de l'enceinte (1) ou similaire entre l'enceinte (1) et l'arbre (2), et qui est ajusté de manière à pouvoir se déplacer dans la direction axiale d'une manière par rapport à l'arbre (2) ; un mécanisme d'affichage de volume (8) qui est entraînée conjointement avec la rotation de la vis à course (7) et est capable d'un affichage variable d'un volume numérique de volume d'admission ; un élément de vis de réglage de volume d'admission variable (9) qui est vissé sur la vis à course (7) et qui détermine un emplacement d'arrêt dans une direction de retour de l'arbre (2). Si la valeur numérique affichée du mécanisme d'affichage de volume (8) et une valeur numérique de volume d'admission réelle sont différentes, puis l'étalonnage est effectué par réglage de la vis à course (7) par rotation manuelle, avec l'élément de vis de réglage de volume d'admission variable (9) étant empêché de tourner, entraînant ainsi le mécanisme d'affichage de volume (8) et amenant la valeur numérique affichée de celui-ci à correspondre à la valeur numérique du volume d'admission réel.
PCT/JP2019/032126 2019-08-16 2019-08-16 Mécanisme d'étalonnage de volume d'admission de pipette, pipette et procédé d'étalonnage de celui-ci WO2021033213A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN201980099267.1A CN114222629B (zh) 2019-08-16 2019-08-16 移液管的吸入容量校正机构、移液管及其校正方法
PCT/JP2019/032126 WO2021033213A1 (fr) 2019-08-16 2019-08-16 Mécanisme d'étalonnage de volume d'admission de pipette, pipette et procédé d'étalonnage de celui-ci
JP2020513940A JP6788311B1 (ja) 2019-08-16 2019-08-16 ピペットの吸入容量校正機構・ピペット及びその校正方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2019/032126 WO2021033213A1 (fr) 2019-08-16 2019-08-16 Mécanisme d'étalonnage de volume d'admission de pipette, pipette et procédé d'étalonnage de celui-ci

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Publication Number Publication Date
WO2021033213A1 true WO2021033213A1 (fr) 2021-02-25

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PCT/JP2019/032126 WO2021033213A1 (fr) 2019-08-16 2019-08-16 Mécanisme d'étalonnage de volume d'admission de pipette, pipette et procédé d'étalonnage de celui-ci

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JP (1) JP6788311B1 (fr)
CN (1) CN114222629B (fr)
WO (1) WO2021033213A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4672857A (en) * 1985-09-10 1987-06-16 Labindustries, Inc. Liquid microdispenser
JPS63141650A (ja) * 1986-11-27 1988-06-14 エリック　マルトードートリー サンプリングおよび計量用ピペット並びにその目盛り定め方法
JPH07185361A (ja) * 1993-10-21 1995-07-25 Eppendorf Geraetebau Netheler & Hinz Gmbh ピペット
JP2013503032A (ja) * 2009-08-28 2013-01-31 ピジェット ハテエル スポルカ アクシイナ 吸引液体の容積値を調節可能な機械式ピペット
US20190083969A1 (en) * 2017-09-19 2019-03-21 Mettler-Toledo Rainin, LLC Pipette quickset volume adjustment mechanism

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6428750B1 (en) * 2000-02-17 2002-08-06 Rainin Instrument, Llc Volume adjustable manual pipette with quick set volume adjustment
PL211690B1 (pl) * 2007-11-23 2012-06-29 Pz Htl Społka Akcyjna Pipeta z nastawianą objętością pobieranej cieczy
JP2011000490A (ja) * 2009-06-16 2011-01-06 Nichiryo Co Ltd ピペット
CN202666878U (zh) * 2012-06-29 2013-01-16 深圳市瑞沃德生命科技有限公司 微量液体加样器调节控制装置
CN205462304U (zh) * 2016-01-27 2016-08-17 柳州市妇幼保健院 一种声音提示移液器
CN207324859U (zh) * 2017-06-12 2018-05-08 郝爱军 一种检验科用移液管

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4672857A (en) * 1985-09-10 1987-06-16 Labindustries, Inc. Liquid microdispenser
JPS63141650A (ja) * 1986-11-27 1988-06-14 エリック　マルトードートリー サンプリングおよび計量用ピペット並びにその目盛り定め方法
JPH07185361A (ja) * 1993-10-21 1995-07-25 Eppendorf Geraetebau Netheler & Hinz Gmbh ピペット
JP2013503032A (ja) * 2009-08-28 2013-01-31 ピジェット ハテエル スポルカ アクシイナ 吸引液体の容積値を調節可能な機械式ピペット
US20190083969A1 (en) * 2017-09-19 2019-03-21 Mettler-Toledo Rainin, LLC Pipette quickset volume adjustment mechanism

Also Published As

Publication number Publication date
CN114222629B (zh) 2023-11-14
JPWO2021033213A1 (ja) 2021-09-13
JP6788311B1 (ja) 2020-11-25
CN114222629A (zh) 2022-03-22

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