WO2020027133A1 - Composition de stérilisation et de dégradation d'acide nucléique - Google Patents

Composition de stérilisation et de dégradation d'acide nucléique Download PDF

Info

Publication number
WO2020027133A1
WO2020027133A1 PCT/JP2019/029834 JP2019029834W WO2020027133A1 WO 2020027133 A1 WO2020027133 A1 WO 2020027133A1 JP 2019029834 W JP2019029834 W JP 2019029834W WO 2020027133 A1 WO2020027133 A1 WO 2020027133A1
Authority
WO
WIPO (PCT)
Prior art keywords
formaldehyde
nucleic acid
concentration
sterilization
formic acid
Prior art date
Application number
PCT/JP2019/029834
Other languages
English (en)
Japanese (ja)
Inventor
利彦 岡崎
鈴木　康士
島垣　昌明
Original Assignee
株式会社シーライブ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社シーライブ filed Critical 株式会社シーライブ
Priority to JP2020531679A priority Critical patent/JPWO2020027133A1/ja
Publication of WO2020027133A1 publication Critical patent/WO2020027133A1/fr

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof

Definitions

  • the present invention relates to a sterilizing / nucleic acid decomposing composition capable of sterilizing bacteria and viruses and decomposing nucleic acids and Lipopolysaccharide (hereinafter, LPS).
  • LPS Lipopolysaccharide
  • nucleic acids which are poorly soluble in water, are unnecessary on solid surfaces such as reaction vessels used and in liquids such as reaction solutions. If the bacteria or cells are unnecessarily mixed into the surface of a solid such as a reaction vessel or a liquid such as a reaction solution, it may have a serious adverse effect on the results of tests and research experiments. May cause. In addition, even though sterilized, residual bacteria and LPS present in cells may have a serious adverse effect on the results of tests and research experiments.
  • a water-absorbing resin described in Patent Document 1 and a germicidal material containing an aqueous formaldehyde solution held by the water-absorbing resin have an excellent germicidal action, and are excellent germicidal materials that can be easily disposed of at low cost. It is described that it can be used as.
  • the sterilizing material described in Patent Document 1 requires an aqueous solution having a formaldehyde concentration of 10 to 50% in order to obtain a sufficient sterilizing effect.
  • High concentrations of formaldehyde are acutely toxic to the human body, mainly irritating to mucous membranes, and vapors are toxic, causing inflammation of the respiratory system, eyes and throat.
  • concentration of formaldehyde was as low as 5% or less, a sufficient bactericidal effect could not be obtained.
  • a sterilization system using a composite gas generated by a catalytic reaction from methanol has a sterilizing power higher than that of ethylene oxide gas (EOG) or ozone which has been frequently used as a gas used for sterilization of medical instruments and the like. It has been confirmed that they have extremely low corrosion resistance and corrosiveness, and because of their excellent permeability and diffusivity, they are currently attracting attention in many fields.
  • EOG ethylene oxide gas
  • Composite gas is a gas that has a strong bactericidal and sterilizing effect generated from methanol by a catalyst.
  • the composite gas has a high permeability, and can be sterilized by permeating the inside of the object to be sterilized even at the atmospheric pressure. Since it is not a mist of contact sterilization, it is excellent in that there is little corrosion of metal and deterioration of plastics (corrosiveness), it does not choose the material of the material to be sterilized, and it is hard to remain in the material to be sterilized (persistence). It has a characteristic, has a wide diffusibility, and can be exposed evenly to every corner, penetrates into small gaps, and can be exposed even in the energized operating state of precision equipment, electronic equipment, etc., and has high utility.
  • the environment temperature at which the effect of nucleic acid decomposition is exerted is set to a body temperature range of 37 ° C., and within a short time of 15 minutes or less, and at a low formaldehyde component concentration, a double helix DNA It has the ability to effectively decompose nucleic acids (disassembled into base chains of 10 base pairs (bp) or less) and can achieve a nucleic acid resolution of 99.99% to 100% as a gas phase nucleic acid decomposition method. On the other hand, the identification and detailed mechanism of the components that cause these effects have not been sufficiently elucidated.
  • LPS is a component of the outer membrane of the cell wall of Gram-negative bacteria and is a substance (glycolipid) composed of lipids and polysaccharides.
  • LPS is an endotoxin (endotoxin) that exerts various biological activities when it acts on cells of other organisms such as humans and animals.
  • the expression of physiological action of LPS is carried out via Toll-like receptor ⁇ TLR ⁇ ⁇ 4 ⁇ (TLR4) present on the cell membrane surface of the host cell.
  • TLR4 Toll-like receptor
  • the method of increasing and / or decreasing the concentration of the immunomodulatory active substance described in Patent Document 4 does not decompose LPS, but increases or decreases the amount by a receptor-mediated adsorption mechanism.
  • LPS is thermally and chemically stable, and cannot be inactivated by an autoclave used for normal sterilization. It is only known that inactivation requires heating at 250 ° C. for 30 minutes or irradiation with radiation such as ⁇ -rays. However, under these conditions, it cannot be applied to equipment made of a material having no heat resistance, and there has not been anything that can be applied to environmental purification that cannot be transported.
  • JP 2013-166729 A Japanese Patent No. 5463378 JP 2001-519817 A JP-T-2003-519503
  • the present invention has been made in view of such circumstances, and enables sterilization and nucleic acid decomposition in a low concentration range of formaldehyde, and sterilization and nucleic acid decomposition capable of exhibiting excellent efficacy in a short time. It is a first object to provide a composition for use, and a second object to decompose LPS.
  • the present inventors have found a specific substance that contributes to sterilization and nucleic acid decomposition by analyzing components in a composite gas, and have completed the present invention by clarifying the mechanism thereof.
  • the inventors of the present application use substantially the same sterilization and nucleic acid decomposition composition, so that the reaction in a liquid state and the reaction in a gas state are longer than the time required for sterilization and nucleic acid decomposition.
  • LPS could be inactivated, but at least Limulus activity could be inactivated, although the necessity of exposure was different.
  • one embodiment of the present invention is a composition for sterilization and nucleic acid decomposition in a liquid state capable of decomposing nucleic acid and sterilizing, which contains at least formaldehyde and formic acid, and has a formaldehyde concentration of 10% by weight or less. And the concentration of formic acid is 1% by weight or less.
  • one embodiment of the present invention may further include methanol, and optionally include derivatives of methanol, formaldehyde, and formic acid.
  • More efficacy can be exhibited by appropriately including methanol, a methanol derivative, a formaldehyde derivative, and a formic acid derivative according to an object to be sterilized and use conditions.
  • one embodiment of the present invention is characterized in that LPS can be further decomposed.
  • Another embodiment of the present invention is a gaseous sterilization / nucleolysis composition capable of decomposing nucleic acids and sterilizing, which contains at least formaldehyde and formic acid.
  • a composite gas containing formaldehyde and formic acid in the gas phase can exhibit excellent nucleic acid resolution in a short time even at a low concentration.
  • another embodiment of the present invention may include methanol, formaldehyde, formic acid, carbon monoxide, carbon dioxide, hydrogen, oxygen and / or a radical thereof.
  • compositions and / or their radical species are optional to contain these compositions and / or their radical species.
  • the concentration of formaldehyde may be 500 ppm or less, and the concentration of formic acid may be 400 ppm or less.
  • LPS in another embodiment of the present invention, LPS can be further decomposed.
  • sterilization and nucleic acid decomposition can be performed in a low concentration range, and excellent effects can be exhibited in a short time.
  • FIG. 1 A) to (E) are diagrams showing the results of DNA resolution when only formaldehyde was used as Comparative Example 1.
  • FIG. 1 A) to (D) are diagrams showing the results of DNA resolution when only formic acid was used as Comparative Example 2.
  • (A) and (E) show formic acid only, (B) and (D) show formaldehyde only, and (C) and (F) show the results of DNA resolution when formaldehyde and formic acid are used in combination as examples.
  • FIG. (A) and (E) show formic acid only, (B) and (D) show formaldehyde only, and (C) and (F) show the results of DNA resolution when formaldehyde and formic acid are used in combination as examples.
  • formaldehyde has a sterilizing effect on microorganisms such as bacteria and fungi, and has been put to practical use in various fields.
  • formaldehyde has long been used in pathology for long-term fixation of living tissues by immersion in formalin, which is an aqueous solution of formaldehyde.
  • techniques for extracting nucleic acids such as DNA and RNA from formalin-fixed tissue have been developed. Its development has been applied to its genetic analysis and has greatly contributed to the advancement of medicine.
  • formaldehyde is also generated endogenously when metabolizing amino acids and xenobiotics, and even those who have not been exposed to formaldehyde have a blood formaldehyde concentration of 2.61 ⁇ 0.14 ⁇ g / g (almost 2.6 ppm) (Initial Risk Assessment Report for Chemical Substances_Independent Administrative Institution Product Evaluation Technology Foundation ⁇ ⁇ Chemical Substance Management Center).
  • a cross-linking reaction of a protein molecule is known as an action of formaldehyde, and an aldehyde group in a formaldehyde molecule binds to an amino group of a protein in a tissue, and further cross-links, thereby impairing the three-dimensional structure of the protein.
  • formalin causes cross-linking of nucleic acids and proteins, so that DNA is susceptible to physical stress and fragmentation occurs.
  • the generated composite gas contains Evaluate the correlation between concentration, reaction time and potency for each of the constituent components individually, and further examine the effect on potency and effect by various mixing ratios, and create a database to reduce the concentration.
  • the present invention has been clarified for the first time for a mechanism (Mode-of-Action) that exerts an effect in a short time and in an extremely short time and optimization conditions.
  • the composition for sterilization and nucleic acid decomposition is a composition for sterilization and nucleic acid decomposition in a liquid state capable of decomposing nucleic acids and sterilizing, and contains at least formaldehyde and formic acid.
  • the concentration of formaldehyde is 10% by weight or less, and the concentration of formic acid is 1% by weight or less.
  • the present invention by mixing formaldehyde and formic acid in the above ratio, highly efficient sterilization and nucleic acid decomposition can be performed even at a low concentration at which each of them does not exert its effect alone.
  • formaldehyde By coexisting formic acid, formaldehyde exhibits sterilization and nucleic acid degrading effects even at a concentration of 10% by weight or less.
  • the concentration of formaldehyde may more preferably be 5% by weight or less (from the viewpoint of the minimum concentration for obtaining the sterilizing and nucleic acid degrading effects in the presence of formic acid).
  • the lower limit of the formaldehyde concentration is approximately 0.1% by weight.
  • the concentration of formic acid to be mixed with formaldehyde is not more than 1% by weight.
  • the lower limit of the concentration of formic acid is also approximately 0.1% by weight.
  • the composition for sterilizing and decomposing nucleic acids according to one embodiment of the present invention is effective for sterilizing bacteria, fungi and viruses and decomposing nucleic acids.
  • DNA and RNA such as bacteria, fungi and viruses can be effectively decomposed in a short time of 5 minutes or less, and in one minute depending on conditions ( 10 bp or less in a base chain), and an extremely high nucleic acid degradation effect can be achieved.
  • composition for sterilization / nucleic acid decomposition may use water such as pure water, alcohols such as methanol and ethanol, other organic solvents, or a mixture thereof as a solvent.
  • the solvent may appropriately contain a buffer for adjusting pH, a preservative, and the like.
  • composition for sterilization and nucleic acid degradation according to one embodiment of the present invention may have the formaldehyde and formic acid concentrations at the time of use as long as they are at the above concentrations. And may be used after being appropriately diluted at the time of use. Alternatively, an embodiment may be used in which each component is divided and stored, and then mixed and used at the time of use.
  • composition for sterilization and nucleic acid degradation further contains methanol, and can optionally contain a derivative of methanol, a derivative of formaldehyde, and a derivative of formic acid.
  • a derivative refers to a compound that has been modified to such an extent that the structure or properties of methanol, formaldehyde, or formic acid are not significantly changed, such as introduction of an arbitrary functional group, oxidation, reduction, or substitution of an atom.
  • the composition for decomposing LPS is a composition in a liquid state capable of decomposing LPS, and contains at least formaldehyde and formic acid, as well as for sterilization and nucleic acid decomposition,
  • the concentration of formaldehyde is 10% by weight or less and the concentration of formic acid is 1% by weight or less.
  • LPS by mixing formaldehyde and formic acid in the above ratio, LPS can be decomposed even at a low concentration at which each of them does not exert its effect alone.
  • formaldehyde exerts an LPS decomposition effect even at a concentration of 10% by weight or less.
  • the concentration of formaldehyde may more preferably be 5% by weight or less (from the viewpoint of the minimum concentration for obtaining the sterilizing and nucleic acid degrading effects in the presence of formic acid).
  • the lower limit of the formaldehyde concentration is approximately 0.03% by weight.
  • the concentration of formic acid to be mixed with formaldehyde is not more than 1% by weight.
  • the lower limit of the concentration of formic acid is also approximately 0.01% by weight.
  • the contact reaction is preferably carried out for about 10 minutes to 2 hours, more preferably for about 15 minutes to 2 hours, whereby LPS in an amount of 100 to 1000 EU (endotoxin unit) per mL is reduced to about half or less. Can be made.
  • the mode of use of the composition for sterilization and nucleic acid decomposition according to one embodiment of the present invention in the liquid phase is not particularly limited, for example, sterilization and nucleic acid decomposition, the composition for sterilization and nucleic acid decomposition or LPS decomposition for the target of LPS decomposition Sterilization, nucleic acid decomposition, and LPS decomposition may be performed by immersion in a solution, or sterilization, nucleic acid decomposition, and LPS decomposition composition may be sprayed on a sterilization, nucleic acid decomposition, and LPS decomposition target to sterilize, nucleic acid decomposition, and LPS decomposition. It may be disassembled.
  • the contact temperature is not particularly limited, and may be room temperature, or may be appropriately heated to about 37 to 50 ° C. and further to about 60 ° C. If the temperature is higher than this, it is necessary to consider the heat resistance of the object to be processed, which is not preferable.
  • the composition for sterilizing and decomposing nucleic acids according to one embodiment of the present invention can be used in a gaseous state. That is, the composition for sterilization and nucleic acid decomposition according to one embodiment of the present invention is a composition for sterilization and nucleic acid decomposition in a gaseous state capable of decomposing and sterilizing nucleic acids, and contains at least formaldehyde and formic acid. .
  • the concentration of formaldehyde is 500 ppm or less
  • the concentration of formic acid is 400 ppm or less.
  • the present inventors have confirmed that when the concentration of formaldehyde is 200 ppm or less and the concentration of formic acid is 100 ppm or less, it effectively acts on microorganisms (BI indicator bacteria) within 5 minutes. .
  • a composite gas containing formaldehyde and formic acid in a predetermined concentration range in a gaseous phase can exhibit excellent sterilization and nucleic acid resolution in a short time even at a low concentration.
  • the composition for decomposing LPS is a composition capable of decomposing LPS in a gaseous state, and contains at least formaldehyde and formic acid as in the case of sterilization and nucleic acid decomposition.
  • the concentration of formaldehyde is 500 ppm or less, and the concentration of formic acid is 400 ppm or less.
  • the contact reaction is preferably performed for about 10 minutes to 2 hours, more preferably for about 15 minutes to 2 hours.
  • LPS in an amount of 100 to 1000 EU (endotoxin unit) per 1 mL can be reduced to about half or less.
  • the method of using the composition for sterilization / nucleic acid decomposition according to one embodiment of the present invention in a gaseous state is not particularly limited as long as it is a composite gas containing formaldehyde and formic acid.
  • the composition for sterilization and nucleic acid decomposition according to an embodiment of the present invention in a liquid state may be vaporized by heating, or formaldehyde and formic acid in a complex gas generated by reacting methanol or the like with a catalyst such as copper. May be set in such a manner that is within a predetermined concentration range.
  • the lower limit of the concentration of formaldehyde and formic acid is not particularly limited, it is approximately 1 ppm.
  • composition for sterilization and nucleic acid decomposition contains methanol, formaldehyde, formic acid, carbon monoxide, carbon dioxide, hydrogen, oxygen, and / or their radicals in the process of converting into a gaseous state. It may be.
  • the mode of use of the composition for sterilization / nucleic acid decomposition according to one embodiment of the present invention in the gas phase is not particularly limited.
  • a sterilization / nucleic acid decomposition target is placed in a closed space such as a chamber, and
  • the sterilizing / nucleic acid decomposing composition according to one embodiment of the present invention can be sterilized / nucleic acid decomposed by filling the vaporized composition.
  • an appropriate humidity 50 to 90 relative humidity
  • sterilization and nucleic acid decomposition may be performed at low humidity (less than 50 relative humidity%). Is also good.
  • the contact temperature is not particularly limited, and may be room temperature, or may be appropriately heated to about 37 to 50 ° C., and further to about 60 ° C. If the temperature is higher than this, it is necessary to consider the heat resistance of the object to be processed, which is not preferable.
  • the composition for sterilization / nucleic acid degradation according to one embodiment of the present invention has an effect alone by combining formaldehyde and formic acid in both the liquid phase and the gas phase. Even in a low-concentration range that does not exert its effect, it has an excellent sterilizing effect and the resolution of nucleic acids (DNA, RNA, etc.) and can exert its effect in a short time.
  • the DNA used for the nucleic acid degradation test was obtained from subcultured HeLa cells (human cervical adenocarcinoma) (American Type Culture Collection: ATCC) according to the manufacturer's protocol of DNeasy Blood & Tissue (Qiagen). The extraction and purification were performed as genomic DNA.
  • the concentration of the purified genomic DNA was measured using Nano-drop (NanoDrop One, Thermo Fisher Scientific), and the dilution concentration was adjusted with UltraPure TM DNase / RNase-Free Distilled Water (DW; Thermo Fisher Scientific).
  • the genomic DNA whose concentration had been adjusted was sonicated using an ultrasonic homogenizer (UR-21P, TOMY) and used as the homogenized DNA sample.
  • Comparative Example 1 DNA resolution using formaldehyde alone
  • the DNA resolution was examined based on the formaldehyde concentration and the reaction time.
  • a 0.5 ml PCR tube was subjected to a preparative adjustment so that each formaldehyde dilution concentration was obtained.
  • a crushed DNA sample was added, and the reaction time was measured with a stopwatch. After the completion of the reaction, the sample was immediately washed and collected using a QIAquick PCR purification kit, and the obtained sample was analyzed using a Bioanalyzer.
  • the DNA degradation effect was determined from the ratio of the measured peak value of the sample to the peak value of the Upper Marker (molecular weight: 17,000) as follows: Classify. Upper Marker peak value 75% or more and 100% or less: Level 1 Upper Marker peak value 50% or more and less than 75%: Level 2 Upper Marker peak value 25% or more and less than 50%: Level 3 Less than 25% of Upper Marker peak value: Level 4
  • FIG. 1 shows the results of DNA resolution using formaldehyde alone.
  • the evaluation results of the DNA resolution using the formaldehyde diluent alone showed no obvious DNA degradation effect in a low concentration range of 0.1% to 5.0% in a reaction time of 1 minute.
  • a formaldehyde diluted solution 10% [FA10%] (hereinafter, “GA” represents formic acid and “FA” represents formaldehyde) is at Level 3 (0.385) at 1 min. (A)), even at 3 min., Level 4 (0.138) (FIG. 1 (B)), and no complete DNA degradation effect was obtained.
  • the concentration was as high as [FA30%]
  • the DNA was completely degraded for 5 min. (FIG. 1 (C)).
  • Comparative Example 2 DNA resolution with formic acid alone
  • the DNA resolution by the concentration of formic acid alone and the reaction time was examined.
  • Dilute formic acid 98.0%, WAKO special grade reagent
  • 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 1.0%, 1.5%, 2.0%, 3.0%, 5.0% formic acid A solution was prepared. Thereafter, the DNA resolution was evaluated in the same manner as in Comparative Example 1.
  • FIG. 2 shows the results of DNA resolution using formic acid alone.
  • the results of the evaluation of the DNA resolution using the formic acid dilution alone showed a slight DNA degradation effect at a 0.1% concentration in a reaction time of 1 minute, and a level 3 (0.296) (FIG. 2 (A)) of DNA at a concentration of 1.0%. No complete degradation effect was obtained, indicating a moderate DNA resolution.
  • a concentration reached a high concentration range of 2.0% or more, a complete DNA degradation effect was observed with a reaction time of 1 minute.
  • a slight (approximately level 1) DNA degradation effect was observed at 0.1% concentration (FIG.
  • Example 1 DNA resolution in a mixture of formaldehyde and formic acid
  • DNA resolution was evaluated using the composition for sterilization and nucleic acid degradation according to one embodiment of the present invention.
  • a concentration range in which formaldehyde and formic acid alone could not obtain a complete DNA degrading effect it was examined whether or not a synergistic effect of a DNA degrading effect could be obtained by mixing them at various concentrations.
  • a synergistic effect of a DNA degrading effect could be obtained by mixing them at various concentrations.
  • Table 2 shows the results of DNA resolution when the formaldehyde and formic acid according to the present invention were used in combination.
  • “ns” indicates that almost no (less than level 1) DNA degrading effect was obtained
  • “1" to “4" indicate the level 1 to level 4 DNA degrading effects
  • “CD” indicates that the DNA degradation effect was almost completely obtained.
  • Example 2 DNA resolution in mixed gas of formaldehyde and formic acid
  • nucleic acid degradation was examined using a mixed gas of formaldehyde and formic acid.
  • Table 3 shows the results of the DNA resolution.
  • the results are shown in Table 4.
  • the amount of LPS in the fourth column from the left in the table is the amount of LPS before decomposition, and the degree of LPS decomposition can be determined by comparison with the amount of LPS after exposure in the second column from the right.
  • Examples 3 to 9 show the results when liquid was used. It can be seen that the amount of each of the LPSs before the decomposition was reduced by about half.
  • Examples 10 to 19 show the results of LPS decomposition when exposed to a mixed gas of formaldehyde and formic acid as shown in Table 4.
  • Examples 10 and 11 were carried out in a state in which an LPS aqueous solution was placed in a 1.5 mL Eppendorf tube and evaporated to dryness in a 58 ° C. drier to deposit LPS in a pellet form on the bottom.
  • the LPS aqueous solution was attached to a PVDF filter (0.4 ⁇ m diameter) and air-dried and used as a sample.
  • the model is a model in which LPS spreads over the entire film and spreads thinner than a pellet-like one. It was clearly shown that the decomposition efficiency was higher than that of the pellet.
  • Example 16 the LPS standard vial (ENDOSAFE (ENDOTOXIN INDICATOR; Catalog # EVV2K, code number 513-87082)) was opened and the cap was removed. And then diluted to a predetermined concentration.
  • Example 19 is similar to Examples 10 and 11, in which LPS in the form of pellets was adhered. The value of the residual amount of LPS when a sample prepared by the same method as in Example 19 was irradiated with 25 kGy of ⁇ -rays was obtained. It was 0.33 EU compared to 0.66 EU, indicating a further decline. By using this method, LPS resolution comparable to that of ⁇ -ray irradiation can be obtained close to the user, which proves to be of great industrial use value.
  • composition for sterilization / nucleic acid decomposition is not limited to those described in the embodiment and each example of the present invention, and various modifications can be made.
  • composition for sterilization / nucleic acid degradation can be used in the fields of advanced medical treatment (cell therapy, gene therapy, regenerative medicine), marine research, aerospace, and crisis management (defense, firefighting). DNA / RNA-free (removal and decontamination of bio-nucleic acid level contamination) in medical care and nursing care, etc. Application to the field is possible.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Abstract

La présente invention a pour objet de mettre en œuvre une composition de stérilisation et de dégradation d'acide nucléique qui permet une stérilisation et une dégradation d'acide nucléique dans une faible gamme de concentrations, et qui peut présenter une excellente efficacité au cours d'une courte période de temps. De plus, un autre objectif de la présente invention est de mettre en œuvre une composition qui peut effectuer une dégradation de lipopolysaccharide (LPS). Cette composition de la présente invention est une composition à l'état liquide pour la stérilisation et la dégradation d'acide nucléique capable de dégradation et de stérilisation d'acide nucléique et contient au moins du formaldéhyde et un acide formique, la concentration de formaldéhyde étant de 10 % en poids ou moins, et la concentration en acide formique étant de 1 % en poids ou moins.
PCT/JP2019/029834 2018-07-30 2019-07-30 Composition de stérilisation et de dégradation d'acide nucléique WO2020027133A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2020531679A JPWO2020027133A1 (ja) 2018-07-30 2019-07-30 滅菌・核酸分解用組成物

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2018142530 2018-07-30
JP2018-142530 2018-07-30

Publications (1)

Publication Number Publication Date
WO2020027133A1 true WO2020027133A1 (fr) 2020-02-06

Family

ID=69231858

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2019/029834 WO2020027133A1 (fr) 2018-07-30 2019-07-30 Composition de stérilisation et de dégradation d'acide nucléique

Country Status (2)

Country Link
JP (3) JPWO2020027133A1 (fr)
WO (1) WO2020027133A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09503383A (ja) * 1993-07-20 1997-04-08 ユニバーシティ オブ カリフォルニア，ロスアンゼルス プロテグリン
JP2002012505A (ja) * 2000-04-28 2002-01-15 Daicel Chem Ind Ltd 固形化ギ酸、黴・細菌汚染防止飼料及びその製造方法
JP2008137962A (ja) * 2006-12-04 2008-06-19 Kyushu Univ 核酸分解剤および核酸の分解方法
JP2011041483A (ja) * 2009-08-19 2011-03-03 Wingturf Co Ltd 核酸分解剤および核酸分解法
JP2013192498A (ja) * 2012-03-19 2013-09-30 Sealive Inc 核酸分解処理装置
JP2015167846A (ja) * 2014-03-04 2015-09-28 株式会社ルナエル 対象物の処理方法及び処理装置

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3965194A (en) * 1973-10-19 1976-06-22 The Lummus Company Production of formaldehyde in carbon steel reactors
DE3143704A1 (de) * 1981-11-04 1983-05-11 Bayer Ag, 5090 Leverkusen Verfahren zur herstellung von formaldehyd
JPH0662478B2 (ja) * 1984-04-12 1994-08-17 三菱瓦斯化学株式会社 ホルムアルデヒド水溶液の製造法
JPH11504842A (ja) * 1995-12-01 1999-05-11 ミンテック・コーポレーション 医療装置のための室温滅菌剤
DE102008064481A1 (de) 2008-12-18 2010-08-12 Bode Chemie Gmbh Kombinierte Desinfektions- und Dekontaminationsmittel mit erhöhter Wirksamkeit
CN104245781B (zh) * 2012-02-20 2018-09-21 巴斯夫欧洲公司 用聚合物提高生物杀伤剂的抗微生物活性
JP6639449B2 (ja) 2017-08-18 2020-02-05 国立大学法人九州工業大学 黄色ブドウ球菌およびセレウス菌用選択性抗菌剤の製造方法。

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09503383A (ja) * 1993-07-20 1997-04-08 ユニバーシティ オブ カリフォルニア，ロスアンゼルス プロテグリン
JP2002012505A (ja) * 2000-04-28 2002-01-15 Daicel Chem Ind Ltd 固形化ギ酸、黴・細菌汚染防止飼料及びその製造方法
JP2008137962A (ja) * 2006-12-04 2008-06-19 Kyushu Univ 核酸分解剤および核酸の分解方法
JP2011041483A (ja) * 2009-08-19 2011-03-03 Wingturf Co Ltd 核酸分解剤および核酸分解法
JP2013192498A (ja) * 2012-03-19 2013-09-30 Sealive Inc 核酸分解処理装置
JP2015167846A (ja) * 2014-03-04 2015-09-28 株式会社ルナエル 対象物の処理方法及び処理装置

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HASHIBA, TOMOHIKO ET. AL.: "4. features of MRG sterilization system, "(B-10) Development of newly invented MRG (Methanol Radical Gas) sterilization system"", PROCEEDINGS OF 27TH ANNUAL TECHNICAL MEETING ON AIR CLEANING AND CONTAMINATION CONTROL PROGRAM, 2009, pages 81 - 84 *
OSHIMA, M.: "The fate of nucleic acid bases in Ladino clover during Ensilage", JAPANESE JOURNAL OF GRASSLAND SCIENCE, vol. 28, no. 3, 1982, pages 302 - 309, XP055681210 *
SUZUKI, KOJI, ET . AL.: "Application to sterilization system by new chemical nucleic acid degradation and decontamination technologies", LECTURE ABSTRACTS OF THE 51ST ANNUAL MEETING OF JAPANESE ASSOCIATION FOR EXPERIMENTAL ANIMAL TEHCNOLOGIES, 2017, pages 83 *

Also Published As

Publication number Publication date
JP2021010378A (ja) 2021-02-04
JPWO2020027133A1 (ja) 2020-09-17
JP7057587B2 (ja) 2022-04-20
JP2021010379A (ja) 2021-02-04
JP7057586B2 (ja) 2022-04-20

Similar Documents

Publication Publication Date Title
Rathore et al. Investigation of physicochemical properties of plasma activated water and its bactericidal efficacy
JPS63502244A (ja) 生物流体の殺菌方法および装置およびその容器
Tyagi et al. Bactericidal action of lemon grass oil vapors and negative air ions
Wales et al. Disinfectant testing for veterinary and agricultural applications: A review
Kwon et al. Potential applications of non-thermal plasma in animal husbandry to improve infrastructure
Potrč et al. Bioactive characterization of packaging foils coated by chitosan and polyphenol colloidal formulations
Phillips et al. Chemical disinfectants
Hirose et al. Viscosity is an important factor of resistance to alcohol-based disinfectants by pathogens present in mucus
Papp et al. Evaluation of sporicidal disinfectants for the disinfection of personal protective equipment during biological hazards
JP7057586B2 (ja) 滅菌かつ核酸分解用組成物
Loizou et al. Effect of cold atmospheric plasma on SARS-CoV-2 inactivation: a pilot study in the hospital environment
Baik et al. Synergistic effect of hydrogen peroxide and cold atmospheric pressure plasma-jet for microbial disinfection
Lemnaru et al. Antimicrobial Wound Dressings based on Bacterial Cellulose and Independently Loaded with Nutmeg and Fir Needle Essential Oils
Marchetti et al. Evaluation of fungicidal efficacy of benzalkonium chloride (Steramina G uv) and Virkon-S against Microsporum canis for environmental disinfection
Tweij-Thu-Alfeqar Razzaq et al. Sterilization of Surgical Tools: Removing Bacterial Endospores with a Combination of Povidone-iodine, Chlorhexidine Gluconate, Ethanol, and Methanol
Wolny-Koładka et al. Eco-friendly approach to the synthesis of silver nanoparticles and their antibacterial activity against Staphylococcus spp. and Escherichia coli
Elgharbawy et al. Antibacterial performance enhancement using hydrophobic deep eutectic solvents: COSMO-RS prediction, experimental validation, and synergistic action with antibiotics
Nomura et al. Performance evaluation of bactericidal effect and endotoxin inactivation by low‐temperature ozone/hydrogen peroxide mixed gas exposure
Butzloff Micro-CT imaging of denatured chitin by silver to explore honey bee and insect pathologies
Suenaga et al. Ultrasonic-combined plasma bubbling for adherent bacteria disinfection on medical equipment
CN113144177A (zh) 一种表面杀菌抗病毒喷剂及其制备方法与应用
Nadruz et al. Efficacy of high‐level disinfection of endoscopes contaminated with Streptococcus equi subspecies equi with 2 different disinfectants
Niemiec et al. Radiant catalytic ionization improves the microbiological status of rodent facilities without affecting the prooxidative status of mice
Marchal et al. Atmospheric cold plasmas for biofilm inactivation: does biofilm extracellular matrix limit the bactericidal process?
Ayub et al. Use of Hydrogen Peroxide Vapour for Microbiological Disinfection in Hospital Environments: A Review

Legal Events

Date Code Title Description
ENP Entry into the national phase

Ref document number: 2020531679

Country of ref document: JP

Kind code of ref document: A

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 19843275

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 19843275

Country of ref document: EP

Kind code of ref document: A1