WO2019235755A1 - 제니핀을 포함하는 전기화학적 센서의 센싱 막 또는 확산제어막의 제조용 가교제 - Google Patents
제니핀을 포함하는 전기화학적 센서의 센싱 막 또는 확산제어막의 제조용 가교제 Download PDFInfo
- Publication number
- WO2019235755A1 WO2019235755A1 PCT/KR2019/006001 KR2019006001W WO2019235755A1 WO 2019235755 A1 WO2019235755 A1 WO 2019235755A1 KR 2019006001 W KR2019006001 W KR 2019006001W WO 2019235755 A1 WO2019235755 A1 WO 2019235755A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- diffusion control
- electrochemical sensor
- film
- crosslinking agent
- sensor
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
- C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/94—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems condensed with rings other than six-membered or with ring systems containing such rings
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/0008—Organic ingredients according to more than one of the "one dot" groups of C08K5/01 - C08K5/59
- C08K5/0016—Plasticisers
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/15—Heterocyclic compounds having oxygen in the ring
- C08K5/151—Heterocyclic compounds having oxygen in the ring having one oxygen atom in the ring
- C08K5/1545—Six-membered rings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/002—Electrode membranes
- C12Q1/003—Functionalisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
Definitions
- the present invention relates to acidic crosslinking agents used in the manufacture of electrochemical sensors, and more particularly to novel uses as crosslinking agents of jennifer pins used in the production of sensing or diffusion control membranes in electrochemical sensors for glucose measurement. .
- biosensors using enzymes are chemical sensors used to selectively detect and measure chemicals contained in a sample by using a biological detection function in which a living organism reacts sensitively to a specific substance such as a functional substance or a microorganism. It has been developed for medical measurement applications such as sensors, and research is being actively conducted in other applications such as food engineering and environmental measurement.
- Periodic measurement of blood glucose is very important in diabetes management, and various blood glucose meters have been manufactured to measure blood glucose easily using a portable instrument.
- the operating principle of the biosensor is based on an optical method or an electrochemical method, and unlike the conventional biosensor, the biochemical biosensor can reduce the influence of oxygen, and even if the sample becomes cloudy, It has the advantage that it can be used without additional pretreatment. Therefore, various kinds of electrochemical biosensors with accuracy and precision are widely used.
- the commercially available electrochemical blood glucose sensor mainly uses enzyme electrodes, and more specifically, has a structure in which glucose oxidase is fixed on an electrode capable of converting electrical signals by chemical or physical methods.
- Such an electrochemical glucose sensor is based on the principle of measuring glucose concentration in an analyte by measuring an electric current generated by transferring electrons generated by oxidation of glucose in an analyte such as blood to an electrode.
- a polymer support is required, and in order to prepare such a polymer support, a crosslinking agent such as glutaraldehyde, ethylene glycol-diglycidyl ether, or the like must be used.
- Such crosslinking agents may also be used in the manufacture of diffusion control membranes used to control or limit the amount of glucose flowing from a sample in vivo.
- CGMS continuous glucose monitoring sensor
- Used in the manufacture of the support or diffusion control membrane may cause a problem that the cross-linking agent, etc. that may be included in the sensor is absorbed by the human body may cause toxicity and side effects, and the use of a safe technology or material that can minimize the need for such The development of safe materials has been called for.
- the present invention provides a sensor material that enables quantitative and qualitative analysis of blood glucose, specifically, the production or diffusion control of a polymer support of a sensing membrane that serves to help stabilize and disperse enzymes (3? 311 33 ⁇ 4) in an electrochemical sensor. It is an object to provide novel crosslinking agents used for the production of membranes.
- the present invention is a cross-linking agent comprising jennypin, used for the production of the polymer support of the sensing film or the production of the diffusion control film in the electrochemical sensor, the sensing film or diffusion control manufactured using the same It provides a film and an electrochemical sensor including the sensing film or diffusion control film.
- the phenomena of the phenomena which can be extracted and used in plants are higher than those of the conventionally toxic crosslinkers.
- the amount of amine group can be easily determined by measuring or measuring the amount of the amine group present. It is very useful in the manufacture of electrochemical sensors in that it has the advantage of reducing the sensor life shortened by high concentration of glucose and at the same time.
- Figure 1 is a graph comparing the change in glucose sensitivity with or without the cross-linking agent containing jenny pin according to the present invention in the production of the sensing film.
- electrode I corresponds to the result of the electrode I) containing the sensing film manufactured using jenny pin as a crosslinking agent.
- Figure 2 is prepared using a crosslinking agent comprising jennifer pin according to the present invention!
- 1 is a graph comparing the change in glucose response according to the diffusion control membrane.
- 13 is a result of electrode 1) having a sensing film prepared using jennypin as a crosslinking agent but no diffusion control film, and a sensing film prepared using jennypin as a crosslinking agent, and jennypin as a diffusion control film.
- the result of the electrode (1) which has the 1 1 diffusion control film manufactured using this as a crosslinking agent is shown.
- Figure 3 is a graph comparing the change in glucose sensitivity according to the presence or absence of gelatin diffusion control film prepared using a cross-linking agent comprising jenny pin according to the present invention.
- Fig. 3, 1 is a result of electrode 13 having a sensing film made using jennypin as a crosslinking agent but no diffusion control film, and a line having a sensing film made using jennypin as a crosslinking agent, and jennypin as a diffusion control film. The result of the electrode line which has the gelatin diffusion control film manufactured using this as a crosslinking agent is shown.
- Figure 4 is a graph comparing the change in glucose sensitivity with or without chitosan diffusion control film prepared by using a cross-linking agent comprising jenny pin according to the present invention.
- 13 is a result of electrode 13 having a sensing film prepared using jennypin as a crosslinking agent but no diffusion control film, and now having a sensing film manufactured using jennypin as a crosslinking agent, jennypin as a diffusion control film.
- the result of the electrode (1) which has a chitosan diffusion control film manufactured using it as a crosslinking agent is shown.
- 5 is a graph confirming the glucose sensitivity during long-term storage in the electrode (1 glucose at a high concentration of using the jenny pin as a crosslinking agent.
- the present invention relates to a crosslinking agent composition for producing a sensing film or a diffusion control film of an electrochemical biosensor comprising jennypin root !!
- derivatives of genipin refers to the same action as the genipin, and includes a compound having a cross-linking purpose for the polymer monomer, without limitation, for example, geniposide (geniposide), Genipiodisic acid, penta-acetyl geniposide; Molecular Pharmacology, 70 (3), 997-1004, 2006), 6a-hydroxygeniposide, 6b-hydroxygeniposide 6b_hydroxygeniposide, 6a-methoxygeniposide, or 6t - methoxygeniposide, 6b - methoxygeniposide (Journal of Health Science, 52 (6), 743-747, 2006), but is not limited thereto.
- the phenomena constitutes a sensing membrane that reacts with a metabolite to be measured by an electrochemical sensor and is reduced to catalyze the redox reaction of a living body, and serves to help stabilize and disperse the enzyme.
- It can be used as a crosslinking agent for fixing to a water-soluble polymer support.
- the polymer support may also be connected to an electron transport medium included in the sensing film, in which case the crosslinking with the Jenny pin using a material containing an amine group (N3 ⁇ 4) at the end of the molecule of the electron transport medium or the electron transport medium complex. It can be made available.
- jennifer pin can be used as a crosslinking agent in the preparation of the water-soluble polymer diffusion control film in the electrochemical sensor.
- the crosslinking agent according to the present invention is a material that can be used in the main line in plants, and has the advantage of being very useful in the manufacture of an electrochemical sensor in that it has a high biocompatibility compared to the conventionally high toxicity crosslinking agents.
- the reaction with a compound having an amine group, for example, an electron transfer medium has the advantage that the progress of the reaction can be easily confirmed by measuring the amount of the amine group or UV measurement present.
- polymers that can use the nippin according to the present invention as a crosslinking agent include a polymer having a heterocycle having an N atom such as polyvinyl pyrrol i done; PVP, polyvinyl imidazole, etc.
- the present invention relates to a sensing film for an electrochemical sensor prepared using the above-mentioned Jenny pin as a crosslinking agent.
- the sensing membrane may include an electron transfer medium and an oxidoreductase, in addition to a polymer support prepared by using jennypin as a crosslinking agent.
- the oxidoreductase refers to an enzyme that catalyzes a redox reaction of a living body, and in the present invention, an enzyme that is reduced by reacting with a target substance to be measured, for example, a biosensor, is measured.
- the reduced enzyme reacts with the electron transfer mediator and quantifies metabolites by measuring signals such as current changes that occur.
- the oxidoreductase usable in the present invention may be one or more selected from the group consisting of various dehydrogenases, oxidases, esterases, and the like, depending on the redox or detection target substance, Among enzymes belonging to the enzyme group, an enzyme having a target substance as a substrate may be selected and used.
- the oxidoreductase is glucose dehydrogenase, glutamate dehydrogenase, glucose oxidase, cholesterol oxidase, cholesterol esterase, lactate Lactate oxidase, ascorbic acid oxidase, alcohol oxidase, al cohol dehydrogenase, bilirubin oxidase, bi 1 i rubin oxidase It may be one or more selected from the group.
- the oxidoreductase is a target material to be measured (eg, Metabolites) may include cofactors that store hydrogen taken from oxidoreductases, such as flavin adenine dinucleotide (FAD), nicotinamide adenine di It may be one or more selected from the group consisting of nucleotides (nicotinamide adenine dinucleotide, NAD), pyrroloquinoline quinone (Pyrroloquinol ine quinone, PQQ).
- FAD flavin adenine dinucleotide
- NAD nicotinamide adenine dinucleotide
- PQQ pyrroloquinoline quinone
- the electron transfer mediator is reduced by redox reaction with the reduced enzyme by reacting with the metabolite.
- the reduced electron transfer mediator thus formed serves to generate current at the electrode surface to which the oxidation potential is applied.
- the electron transfer medium may be one kind or two or more kinds, and may be a metal-containing complex including one or more kinds selected from the group consisting of Ru, Fe, Os, Rh, Mo, and Ir, but is not limited thereto.
- the present invention relates to an electrochemical sensor prepared using the above-mentioned jennypin as a crosslinking agent.
- the electrochemical sensor may preferably be an electrochemical sensor for measuring blood glucose, and more preferably a continuous blood glucose monitoring sensor.
- the type of the electrochemical biosensor is not limited, but preferably may be a continuous blood glucose monitoring sensor.
- the present invention is, for example, an electrode, an insulator, a substrate, a sensing layer including the electron transfer mediator and a redox enzyme, a diffusion control layer. ), A protection layer, and the like.
- an electrode it may include two kinds of electrodes, such as a working electrode and a counter electrode, and may include three kinds of electrodes, such as a working electrode, a counter electrode, and a reference electrode.
- the biosensor according to the present invention comprises a reagent composition comprising an electron transfer medium and an enzyme capable of redoxing a liquid biological sample on a substrate having at least two, preferably two or three electrodes. It may be an electrochemical biosensor prepared by drying after coating.
- the working electrode and the counter electrode are provided on opposite sides of the substrate, and the jenny pin according to the present invention is placed on the working electrode. 2019/235755 1 »(: 1 ⁇ 1 ⁇ 2019/006001
- a cross-linked sensing film is laminated, and a planar electrochemical biosensor is laminated on both sides of a substrate provided with a working electrode and a counter electrode, and then a diffusion control film and a protective film cross-linked using insulators and jennypins in turn.
- the substrate is grave 1 '(1) 01761;? ? 1171611 ⁇ 2 ⁇ muscle, 1 (£) 01 31 011 6) and the material at least one selected from the group consisting of: 1 (1301 11 ⁇ 16 It may have been.
- the working electrode may also be a carbon, gold, platinum, silver or silver / silver chloride electrode.
- gold, platinum, silver, or silver / silver chloride electrodes may be used as the counter electrode.
- gold, platinum, silver, or silver / silver chloride electrodes may be used as reference electrodes, and carbon electrodes may be used as counter electrodes.
- the present invention relates to a method for manufacturing an electrochemical biosensor, comprising the step of preparing a sensing film or diffusion control film using jennypin as a crosslinking agent.
- the manufacturing method of the electrochemical biosensor according to the present invention is characterized in that the use of Jenny pin as a crosslinking agent for the production of the sensing film or diffusion control film, specific manufacturing process, reagents, etc.
- the present inventors synthesize the osmium-polymer 1 1-030 7) 2 ( ⁇ 1 as an electron transfer medium by using the phenine as a cross-linking agent to prepare a sensing film using the same as the cross-linking agent
- the operating electrode was manufactured by manufacturing a diffusion control film using the same, and the sensing electrode including the osmite-polymer manufactured without using a crosslinking agent and the operating electrode using the diffusion control film were manufactured to confirm the performance as an electrochemical sensor.
- the sensing film using the crosslinking agent containing the Jenny pin is applied to the electrode, the sensing film is effectively formed on the surface of the electrode to increase the sensitivity, and the diffusion control film using the crosslinking agent containing the Jenny pin is applied to the electrode.
- the diffusion control film using the crosslinking agent containing the Jenny pin is applied to the electrode.
- an osmium polymer (PVI-0s (bpy) 2 Cl 2 ) having the following Chemical Formula 2 was prepared.
- PVI-0s (bpy) 2 Cl was synthesized according to Scheme 1 using 0s (bpy) 2 Cl 2 ) prepared in Reference Example 1-1.
- Weight ratio 10 mg was dissolved in 1 mL of 0.1M acetic acid buffer; pH 5.0 solution.
- the working electrode was manufactured using the sensing film and the diffusion control film prepared in Examples 1 and 2.
- a screen printed carbon electrode (diameter: 5 mm) was used, and in the case of electrodes a and b, the sensing film prepared in Example 1 was placed on the carbon electrode and dried to prepare a working electrode.
- the electrodes c, d, and e and the sensing film prepared in Example 1 were placed on the carbon electrode, respectively, and dried, and then the diffusion control film prepared in Example 2 was laminated on the sensing film, respectively, and dried to prepare a working electrode.
- the electrode including the sensing film and / or diffusion control film prepared in Examples 1 and 2 was immersed for 30 minutes. Once the stabilization is complete, add a high concentration of glucose solution to the seedlings.
- the electrode comprising a sensing film prepared by using a cross-linking agent comprising an electrode 3 and a nippin containing a sensing film does not contain a crosslinking agent according to the present invention, each containing glucose When immersed in the buffer solution, it was confirmed that the electrode 3 produced without the crosslinking agent continuously eluted at the electrode surface.
- the sensitivity of the sensing film produced using the crosslinking agent was 7.1 times higher than that of the sensing film produced without using the crosslinking agent.
- the sensing film on the surface of the electrode than when not using the crosslinking agent 2019/235755 1 »(: 1 ⁇ 1 ⁇ 2019/006001
- the diffusion prepared by crosslinking the cross-linking agent jinipine and chitosan had a sensitivity of about 1/10 lower than that of the electrode 1) without the diffusion control film.
- the sensing film using the cross-linking agent containing Jenny pin is applied to the electrode, the sensing film is effectively formed on the surface of the electrode and the sensitivity is increased, and the diffusion control film using the cross-linking agent containing Jenny pin is used.
- the performance was confirmed.
- the electrical signals of the sensors were measured according to glucose concentrations (1, 2, 3, 4, and 53 ⁇ 4) by the same method as Experimental Example 1, and then the sensor was buffered containing a high concentration of glucose (5). After soaking in solution, the solution was stored for 3 days while applying 0.35 at the applied potential. The sensitivity of the sensor according to the concentration was confirmed using the electrode stored in the high concentration of glucose on the 2nd and 3rd day of storage, respectively, as shown in FIG. 5.
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Hematology (AREA)
- Emergency Medicine (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2019283368A AU2019283368B2 (en) | 2018-06-08 | 2019-05-09 | Crosslinker comprising genipin for use in preparation of sensing film or diffusion control film of electrochemical sensor |
US16/972,540 US20220057355A1 (en) | 2018-06-08 | 2019-05-09 | Crosslinker comprising genipin for use in preparation of sensing film or diffusion control film of electrochemical sensor |
JP2020567131A JP7160950B2 (ja) | 2018-06-08 | 2019-05-09 | ゲニピンを含む電気化学的センサのセンシング膜または拡散制御膜の製造用架橋剤 |
EP19814903.1A EP3805304A4 (en) | 2018-06-08 | 2019-05-09 | CROSSLINKER WITH GENIPIN FOR USE IN MAKING A SENSOR FILM OR A DIFFUSION CONTROL FILM OF AN ELECTROCHEMICAL SENSOR |
NZ770823A NZ770823A (en) | 2018-06-08 | 2019-05-09 | Crosslinker comprising genipin for use in preparation of sensing film or diffusion control film of electrochemical sensor |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20180066188 | 2018-06-08 | ||
KR10-2018-0066188 | 2018-06-08 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2019235755A1 true WO2019235755A1 (ko) | 2019-12-12 |
Family
ID=68770483
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2019/006001 WO2019235755A1 (ko) | 2018-06-08 | 2019-05-09 | 제니핀을 포함하는 전기화학적 센서의 센싱 막 또는 확산제어막의 제조용 가교제 |
Country Status (7)
Country | Link |
---|---|
US (1) | US20220057355A1 (ko) |
EP (1) | EP3805304A4 (ko) |
JP (1) | JP7160950B2 (ko) |
KR (1) | KR102188664B1 (ko) |
AU (1) | AU2019283368B2 (ko) |
NZ (1) | NZ770823A (ko) |
WO (1) | WO2019235755A1 (ko) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022037439A1 (zh) * | 2020-08-18 | 2022-02-24 | 微泰医疗器械(杭州)有限公司 | 一种葡萄糖电化学传感器及其制备方法 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114923965B (zh) * | 2022-03-18 | 2023-05-23 | 杭州微策生物技术股份有限公司 | 一种一体式多指标传感器及其制作方法 |
CN114740068A (zh) * | 2022-04-15 | 2022-07-12 | 深圳可孚生物科技有限公司 | 一种基于Genipin的葡萄糖传感器的制备方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100203144A1 (en) * | 2007-08-23 | 2010-08-12 | University Of Virginia Patent Foundation | Immobilized Metallic Nanoparticles as Unique Materials for Therapeutic and Biosensor Applications |
US8663686B2 (en) * | 2005-05-09 | 2014-03-04 | University Of Washington | Biodegradable chitosan-PEG compositions and methods of use |
KR101512566B1 (ko) * | 2013-10-10 | 2015-04-15 | 광운대학교 산학협력단 | 연속 혈당 모니터링 센서 |
KR20160107464A (ko) * | 2015-03-04 | 2016-09-19 | 삼성전자주식회사 | 효소 기반의 전위차법 글루코스 검출용 센서 및 이의 제조방법 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0716409B2 (ja) * | 1986-12-19 | 1995-03-01 | サントリー株式会社 | 酵素を固定化する方法 |
CN101360449A (zh) * | 2006-02-27 | 2009-02-04 | 爱德华兹生命科学公司 | 用于静脉内电流分析生物传感器的水凝胶 |
JP5164656B2 (ja) * | 2008-04-25 | 2013-03-21 | 株式会社船井電機新応用技術研究所 | センサ及びバイオセンサ |
KR20130084377A (ko) * | 2012-01-17 | 2013-07-25 | 서강대학교산학협력단 | 효소 고정 방법, 이에 의한 효소 센서 및 이를 포함하는 바이오센서 |
KR101694982B1 (ko) * | 2014-12-31 | 2017-01-10 | 주식회사 아이센스 | 전기화학적 바이오센서 |
-
2019
- 2019-05-09 NZ NZ770823A patent/NZ770823A/en unknown
- 2019-05-09 KR KR1020190054489A patent/KR102188664B1/ko active IP Right Grant
- 2019-05-09 WO PCT/KR2019/006001 patent/WO2019235755A1/ko unknown
- 2019-05-09 EP EP19814903.1A patent/EP3805304A4/en active Pending
- 2019-05-09 AU AU2019283368A patent/AU2019283368B2/en active Active
- 2019-05-09 US US16/972,540 patent/US20220057355A1/en active Pending
- 2019-05-09 JP JP2020567131A patent/JP7160950B2/ja active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8663686B2 (en) * | 2005-05-09 | 2014-03-04 | University Of Washington | Biodegradable chitosan-PEG compositions and methods of use |
US20100203144A1 (en) * | 2007-08-23 | 2010-08-12 | University Of Virginia Patent Foundation | Immobilized Metallic Nanoparticles as Unique Materials for Therapeutic and Biosensor Applications |
KR101512566B1 (ko) * | 2013-10-10 | 2015-04-15 | 광운대학교 산학협력단 | 연속 혈당 모니터링 센서 |
KR20160107464A (ko) * | 2015-03-04 | 2016-09-19 | 삼성전자주식회사 | 효소 기반의 전위차법 글루코스 검출용 센서 및 이의 제조방법 |
Non-Patent Citations (4)
Title |
---|
FERNANDES, S. C ET AL.: "Genipin-Cross-Linked Chitosan as a Support for Laccase Biosensor", ELECTROANALYSIS, vol. 25, no. 2, 24 January 2013 (2013-01-24), pages 557 - 566, XP055661702 * |
JOURNAL OF HEALTH SCIENCE, vol. 52, no. 6, 2006, pages 743 - 747 |
MOLECULAR PHARMACOLOGY, vol. 70, no. 3, 2006, pages 997 - 1004 |
See also references of EP3805304A4 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022037439A1 (zh) * | 2020-08-18 | 2022-02-24 | 微泰医疗器械(杭州)有限公司 | 一种葡萄糖电化学传感器及其制备方法 |
Also Published As
Publication number | Publication date |
---|---|
AU2019283368B2 (en) | 2022-08-25 |
EP3805304A4 (en) | 2022-03-02 |
EP3805304A1 (en) | 2021-04-14 |
NZ770823A (en) | 2023-12-22 |
KR102188664B1 (ko) | 2020-12-08 |
AU2019283368A1 (en) | 2021-01-07 |
KR20190139758A (ko) | 2019-12-18 |
JP2021525371A (ja) | 2021-09-24 |
US20220057355A1 (en) | 2022-02-24 |
JP7160950B2 (ja) | 2022-10-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
RU2735654C1 (ru) | Способ и устройство для обнаружения аналита с использованием электрохимического биосенсора | |
TWI559001B (zh) | 生物感測器用之製劑組成物及具有該製劑組成物之生物感測器 | |
JP5275494B2 (ja) | 分析対象物を検出するための試薬及び方法ならびに分析対象物を検出するための試薬を含む装置 | |
JP5947901B2 (ja) | 電気化学バイオセンサーのための酸化還元試薬組成物およびそれを含むバイオセンサー | |
CN101558296B (zh) | 用于测定全血中的1,5-脱水葡萄糖醇的方法以及用于所述方法的传感器芯片和测定试剂盒 | |
US10330634B2 (en) | Non-enzymatic glucose sensor | |
JP7324871B2 (ja) | 電気化学的バイオセンサ用センシング膜、電気化学的バイオセンサ | |
WO2019235755A1 (ko) | 제니핀을 포함하는 전기화학적 센서의 센싱 막 또는 확산제어막의 제조용 가교제 | |
EP3854827B1 (en) | Oxidation-reduction polymer including transition metal complex, and electrochemical biosensor using same | |
KR101694982B1 (ko) | 전기화학적 바이오센서 | |
KR20190143644A (ko) | 신규 루테늄계 전자전달 매개체를 포함하는 산화환원반응용 시약조성물 및 바이오센서 | |
KR20190123169A (ko) | 신규한 전이금속 복합체를 포함하는 산화-환원 고분자 및 이를 이용한 전기화학적 바이오센서 | |
KR20150068223A (ko) | 전기화학적 바이오센서용 산화환원반응 시약조성물 및 이를 이용한 바이오센서 | |
JP2024518294A (ja) | 遷移金属錯体または酸化-還元重合体を含む電気化学的バイオセンサーまたは電気化学的バイオセンサー用センシング膜 | |
KR102266197B1 (ko) | 글루코스 탈수소화효소 감응형 신규 루테늄계 전자전달 매개체, 이의 제조방법, 이를 포함하는 산화환원반응용 시약조성물 및 바이오센서 | |
EP3656868B1 (en) | Glucose monitoring method and glucose sensor | |
JP7449691B2 (ja) | 電気化学測定用の試薬組成物及びその用途 | |
KR102589294B1 (ko) | 작동전극, 이의 제조방법 및 이를 포함하는 바이오센서 및 생체연료전지 | |
CN114894865A (zh) | 一种高精度葡萄糖传感器及其制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 19814903 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 2020567131 Country of ref document: JP Kind code of ref document: A |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
ENP | Entry into the national phase |
Ref document number: 2019283368 Country of ref document: AU Date of ref document: 20190509 Kind code of ref document: A |
|
ENP | Entry into the national phase |
Ref document number: 2019814903 Country of ref document: EP Effective date: 20210111 |