WO2019203540A3 - 다중불포화지방산의 멀티-하이드록시 유도체 생산 방법 - Google Patents

다중불포화지방산의 멀티-하이드록시 유도체 생산 방법 Download PDF

Info

Publication number
WO2019203540A3
WO2019203540A3 PCT/KR2019/004583 KR2019004583W WO2019203540A3 WO 2019203540 A3 WO2019203540 A3 WO 2019203540A3 KR 2019004583 W KR2019004583 W KR 2019004583W WO 2019203540 A3 WO2019203540 A3 WO 2019203540A3
Authority
WO
WIPO (PCT)
Prior art keywords
fatty acids
polyunsaturated fatty
hydroxy derivatives
producing multi
producing
Prior art date
Application number
PCT/KR2019/004583
Other languages
English (en)
French (fr)
Other versions
WO2019203540A2 (ko
Inventor
서정우
이종재
허선연
김영배
김철호
오백록
주정현
최학선
Original Assignee
한국생명공학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020180142896A external-priority patent/KR20190127527A/ko
Priority claimed from KR1020180142895A external-priority patent/KR20190120687A/ko
Application filed by 한국생명공학연구원 filed Critical 한국생명공학연구원
Priority to EP19788644.3A priority Critical patent/EP3792351A4/en
Priority to CN201980025785.9A priority patent/CN111989399A/zh
Priority to JP2020557162A priority patent/JP7490566B2/ja
Publication of WO2019203540A2 publication Critical patent/WO2019203540A2/ko
Publication of WO2019203540A3 publication Critical patent/WO2019203540A3/ko
Priority to US17/072,696 priority patent/US11987833B2/en
Priority to JP2023222379A priority patent/JP2024029151A/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
    • C11C3/00—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 다중불포화지방산으로부터 멀티-하이드록시 유도체를 생성할 수 있는 신규 효소 및 이를 이용한 다중불포화지방산의 멀티-하이드록시 유도체의 생산 방법에 관한 것으로, 상기 효소는 다중불포화지방산을 기질로 이용하여 다중불포화지방산의 멀티-하이드록시 유도체를 단 한번의 반응으로 생산해 낼 수 있는바, 다중불포화지방산의 멀티-하이드록시 유도체의 생체 외 생산에 매우 유용하게 이용될 수 있다.
PCT/KR2019/004583 2018-04-16 2019-04-16 다중불포화지방산의 멀티-하이드록시 유도체 생산 방법 WO2019203540A2 (ko)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP19788644.3A EP3792351A4 (en) 2018-04-16 2019-04-16 PROCESS FOR THE PREPARATION OF MULTI-HYDROXY DERIVATIVES OF POLYUNSATURATED FATTY ACIDS
CN201980025785.9A CN111989399A (zh) 2018-04-16 2019-04-16 多不饱和脂肪酸的多羟基衍生物的制备方法
JP2020557162A JP7490566B2 (ja) 2018-04-16 2019-04-16 多価不飽和脂肪酸のマルチヒドロキシ誘導体の生産方法
US17/072,696 US11987833B2 (en) 2018-04-16 2020-10-16 Method for producing multi-hydroxy derivatives of polyunsaturated fatty acids
JP2023222379A JP2024029151A (ja) 2018-04-16 2023-12-28 多価不飽和脂肪酸のマルチヒドロキシ誘導体の生産方法

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
KR10-2018-0043972 2018-04-16
KR20180043972 2018-04-16
KR20180051695 2018-05-04
KR10-2018-0051695 2018-05-04
KR1020180142896A KR20190127527A (ko) 2018-05-04 2018-11-19 다중불포화지방산의 디-하이드록시 유도체 생산 방법
KR10-2018-0142895 2018-11-19
KR10-2018-0142896 2018-11-19
KR1020180142895A KR20190120687A (ko) 2018-04-16 2018-11-19 다중불포화지방산의 테트라-하이드록시 유도체 생산 방법

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US17/072,696 Continuation-In-Part US11987833B2 (en) 2018-04-16 2020-10-16 Method for producing multi-hydroxy derivatives of polyunsaturated fatty acids

Publications (2)

Publication Number Publication Date
WO2019203540A2 WO2019203540A2 (ko) 2019-10-24
WO2019203540A3 true WO2019203540A3 (ko) 2020-07-16

Family

ID=73441954

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2019/004583 WO2019203540A2 (ko) 2018-04-16 2019-04-16 다중불포화지방산의 멀티-하이드록시 유도체 생산 방법

Country Status (5)

Country Link
US (1) US11987833B2 (ko)
EP (1) EP3792351A4 (ko)
JP (2) JP7490566B2 (ko)
CN (1) CN111989399A (ko)
WO (1) WO2019203540A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4071241A4 (en) * 2019-12-06 2023-10-18 Korea Research Institute of Bioscience and Biotechnology MONO- OR DIHYDROXY DERIVATIVES OF POLY UNSATURATED FATTY ACIDS, METHOD FOR THEIR PRODUCTION AND THEIR USE

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012026106A1 (ja) * 2010-08-25 2012-03-01 国立大学法人 東京大学 光学活性な新規抗炎症性化合物及びその製造方法
US20120059061A1 (en) * 2009-02-20 2012-03-08 The University Of Tokyo Novel anti-inflammatory compounds

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050075398A1 (en) 2003-08-05 2005-04-07 Bazan Nicolas G. Neuroprotectin D1 protects against cellular apoptosis, stroke damage, alzheimer's disease and retinal diseases
EP1720988B1 (de) 2004-02-27 2011-10-12 BASF Plant Science GmbH Verfahren zur herstellung von ungesättigten omega-3-fettsäuren in transgenen organismen
EP1824809A4 (en) 2004-11-19 2010-03-03 Martek Biosciences Corp OXYLIPINES OF LONG-CHAIN, MULTIPLE-UNSATURATED FATTY ACIDS AND METHOD FOR THE PRODUCTION AND USE THEREOF
EP2120920A4 (en) * 2007-02-20 2011-06-15 Martek Biosciences Corp OXYLIPINES OF LONG-CHAIN MULTIPLE-UNSATURATED FATTY ACIDS AND METHOD FOR THEIR PREPARATION AND USE
ES2792676T3 (es) 2008-09-16 2020-11-11 Brigham & Womens Hospital Inc Compuestos del ácido 14-hidroxi-docosahexaenoico
JP2013500275A (ja) 2009-07-29 2013-01-07 フェノメノーム ディスカバリーズ インク ヒドロキシ脂肪酸化合物並びに疾患治療及び診断のためのその使用
CA2873055C (en) 2012-05-10 2023-10-10 Solutex Na Llc Oils with anti-inflammatory activity containing natural specialized proresolving mediators and their precursors
WO2014179207A2 (en) 2013-04-29 2014-11-06 US NewWin, Inc. Enhanced heterologous production of lipoxygenases
SI2984490T1 (sl) 2013-06-14 2020-10-30 Genomatica, Inc. Metode proizvodnje derivatov omega hidroksilirane maščobne kisline
JP6573119B2 (ja) * 2014-01-24 2019-09-11 国立大学法人京都大学 新規酵素を用いた希少脂肪酸の製造法ならびに新規希少脂肪酸
KR101642583B1 (ko) 2014-08-06 2016-07-26 건국대학교 산학협력단 버콜데리아 타일란덴시스 유래 지방산화효소에 의한 13-수산화 리놀레익산의 고수율 제조방법 및 그 조성물
CN113286890A (zh) * 2018-10-19 2021-08-20 弗门尼舍有限公司 脂氧合酶催化从多不饱和脂肪酸(pufa)产生不饱和c10-醛
EP4071241A4 (en) * 2019-12-06 2023-10-18 Korea Research Institute of Bioscience and Biotechnology MONO- OR DIHYDROXY DERIVATIVES OF POLY UNSATURATED FATTY ACIDS, METHOD FOR THEIR PRODUCTION AND THEIR USE

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120059061A1 (en) * 2009-02-20 2012-03-08 The University Of Tokyo Novel anti-inflammatory compounds
WO2012026106A1 (ja) * 2010-08-25 2012-03-01 国立大学法人 東京大学 光学活性な新規抗炎症性化合物及びその製造方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE Protein 7 November 2015 (2015-11-07), "arachidonate 15-lipoxygenase [Oscillatoria nigro-viridis]", XP055724457, retrieved from NCBI Database accession no. WP_015178512.1 *
ELEANOR P. DOBSON, COLIN J. BARROW, JAROSLAV A. KRALOVEC, JACQUI L. ADCOC: "Controlled formation of mono- and dihydroxy-resolvins from EPA and DHA using soybean 15-lipoxygenase", JOURNAL OF LIPID RESEARCH, vol. 54, no. 5, 2013, pages 1439 - 1447, XP055724458 *
JOSEPH T. O’FLAHERTY, YUNGPING HU, RHONDA E. WOOTEN, DAVID A. HORITA, MICHAEL P. SAMUEL, MICHAEL J. THOMAS, HAIGUO SUN, IRIS J. ED: "15-lipoxygenase metabolites of docosahexaenoic acid inhibit prostate cancer cell proliferation and survival", PLOS ONE, vol. 7, no. 9, 20 September 2012 (2012-09-20), pages e45480, XP055724461 *

Also Published As

Publication number Publication date
US20210040518A1 (en) 2021-02-11
JP7490566B2 (ja) 2024-05-27
CN111989399A (zh) 2020-11-24
US11987833B2 (en) 2024-05-21
WO2019203540A2 (ko) 2019-10-24
EP3792351A4 (en) 2022-01-19
JP2021521783A (ja) 2021-08-30
EP3792351A2 (en) 2021-03-17
JP2024029151A (ja) 2024-03-05

Similar Documents

Publication Publication Date Title
MX356086B (es) Produccion de acidos grasos y derivados de los mismos.
WO2017085167A3 (en) Process for producing isobutene from 3-methylcrotonic acid
WO2005103253A8 (en) Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
EP2840131A3 (en) Production of fatty acids and derivatives thereof
WO2016122887A8 (en) Oligosaccharide compositions for use as animal feed and methods of producing thereof
SG10201810439SA (en) Production of long chain polyunsaturated fatty acids in plant cells
BR112016002067A2 (pt) Variantes de enzimas
WO2006055965A3 (en) Oxylipins from long chain polyunsaturated fatty acids and methods of making and using the same
CA2864676C (en) Methods and kits for reducing non-specific nucleic acid amplification
WO2007130390A3 (en) Process compositions and production of acyl alkylisethionate compositions
WO2010023202A3 (en) Nucleic acids encoding desaturases and modified plant oil
EP3502263A3 (en) Method for producing objective substance
ZA200801983B (en) Enzymatic production of (METH) acrylic acid esters
WO2006081644A3 (en) Catalytic process for the esterification of fatty acids
WO2014093505A8 (en) Acp-mediated production of fatty acid derivatives
EP2787083A3 (en) Efficient production of peptides
WO2007133425A3 (en) Fungal delta12 desaturase and delta15 desaturase motifs
MX2014006478A (es) Composiciones que comprenden acidos grasos de carbono-20 y metodos para elaborar y usar los mismos.
MY148450A (en) Process for the preparation of citric acid employing filamentous fungi in a culture medium comprising glycerol
SG10201810616RA (en) Process for producing alkanes using microorganisms combined with kolbe synthesis
JP2015130873A5 (ko)
MY151063A (en) Process for producing a hydrocarbon component
WO2020225504A3 (fr) Substrat pulverulent obtenu par vapocraquage d'une biomasse sans auxiliaire chimique et ses utilisations
WO2019203540A3 (ko) 다중불포화지방산의 멀티-하이드록시 유도체 생산 방법
WO2018012941A3 (ko) 가스발생량이 낮은 류코노스톡 메센테로이드 cjlm119 균주 및 이를 이용한 김치의 제조방법

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 19788644

Country of ref document: EP

Kind code of ref document: A2

ENP Entry into the national phase

Ref document number: 2020557162

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2019788644

Country of ref document: EP

Effective date: 20201116