WO2019198735A1 - 植物の土壌伝染性病害防除方法 - Google Patents

植物の土壌伝染性病害防除方法 Download PDF

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Publication number
WO2019198735A1
WO2019198735A1 PCT/JP2019/015543 JP2019015543W WO2019198735A1 WO 2019198735 A1 WO2019198735 A1 WO 2019198735A1 JP 2019015543 W JP2019015543 W JP 2019015543W WO 2019198735 A1 WO2019198735 A1 WO 2019198735A1
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Prior art keywords
glutamic acid
pseudomonas
soil
present
bacteria
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English (en)
French (fr)
Japanese (ja)
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香純 竹内
茂美 瀬尾
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National Agriculture and Food Research Organization
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National Agriculture and Food Research Organization
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Priority to EP19785141.3A priority Critical patent/EP3782469A4/en
Priority to US17/046,726 priority patent/US20210161149A1/en
Publication of WO2019198735A1 publication Critical patent/WO2019198735A1/ja
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27—Pseudomonas
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids

Definitions

  • the present invention relates to a method for controlling plants from soil-borne diseases. More specifically, the present invention relates to a method for controlling soil-borne diseases of plants using Pseudomonas bacteria and glutamic acid, and a composition and kit that can be used in the method.
  • Chemical chemical pesticides such as fungicides and insecticides are widely used for the purpose of protecting plant diseases.
  • chemical pesticides have a high plant protection effect, there is a possibility that the load on the environment is high, and the plant and the human body are adversely affected by exposure to residual pesticides and pesticides.
  • plant-protecting bacteria bacteria that have an effect of protecting plants from diseases
  • plant-protecting bacteria have been used as biological pesticides in order to eliminate concerns about such chemical pesticides.
  • plant-protecting bacteria several strains of bacteria belonging to the genus Pseudomonas are actually used as microbial pesticides.
  • Specific products include cell seedling spirit (Taki Chemical, Patent Document 1), Veggie Keeper (registered trademark) water Examples include a summing agent (central glass).
  • Plant pest control technology with biological pesticides has many advantages such as low environmental impact, but because it uses biological functions, its effects are not sustained compared to chemical pesticides.
  • problems unique to biological materials such as difficult handling as agricultural chemicals. Therefore, it is desired to make further improvements while deepening the understanding of the ecology and phenotype of the microorganisms used.
  • an object of this invention is to provide the method, composition, and kit which control a plant effectively from a soil infectious disease in the system using microorganisms.
  • the present inventors have focused on Pseudomonas bacteria as useful microorganisms and have intensively studied substances that enhance the plant protective action of the bacteria. I found it effective. Based on this knowledge, the present inventor has completed the present invention.
  • a method for controlling soil infectious diseases of plants comprising a step of applying Pseudomonas bacteria and glutamic acid to soil or plants.
  • Aspect 2 The method according to Aspect 1, wherein the plant is a seed or a seedling.
  • Aspect 3 The method according to Aspect 1 or 2, wherein the plant is a rooted seed.
  • Aspect 4 The method according to any one of Aspects 1 to 3, wherein the Pseudomonas bacterium and glutamic acid are imparted before planting or within 3 days after sowing.
  • Aspect 5 The method according to any one of Aspects 1 to 4, wherein one of Pseudomonas bacteria and glutamic acid is applied, and the other is applied within 3 days after the application.
  • Aspect 6 The method according to any one of Aspects 1 to 5, wherein a Pseudomonas bacterium is provided first, followed by glutamic acid.
  • Aspect 7 The method according to any one of Aspects 1 to 6, further comprising the step of adding glutamic acid after adding Pseudomonas bacteria and glutamic acid.
  • a plant soil-borne disease control kit comprising a Pseudomonas genus bacterium and glutamic acid.
  • plants can be effectively controlled from soil infectious diseases.
  • Pseudomonas bacteria used in the present invention have a track record of use as biological pesticides, and glutamic acid is a kind of natural amino acids. Therefore, the technique provided by the present invention is considered to have high safety against the use of general chemical pesticides. Moreover, in this invention, since the plant protection effect is show
  • FIG. 1 is a photograph showing the disease control effect of Pseudomonas bacteria and glutamic acid against the pathogenic microorganism Picium. From the left of the photograph, water treatment, glutamic acid alone treatment, Pseudomonas bacteria alone treatment, and both treated are shown.
  • FIG. 2 is a graph showing the results of examining the effectiveness of glutamic acid (Glu) and histidine (His) for the combined effect with Pseudomonas bacteria.
  • FIG. 3 is a graph showing the results of examining the effectiveness of a plurality of types of amino acids with respect to the combined effect with Pseudomonas bacteria.
  • FIG. 4 shows Pseudomonas sp.
  • FIG. 5 is a graph showing the disease control effect of Pseudomonas bacteria and glutamic acid against the pathogenic microorganism Rhizoctonia.
  • One embodiment of the present invention is a method for controlling soil-borne diseases of plants, which includes a step of imparting Pseudomonas bacteria and glutamic acid to soil or plants.
  • the Pseudomonas genus bacterium is a bacterium belonging to the family Proteobacterium gamma proteobacteria Pseudomonas and is a gram-negative aerobic gonococcus. It is known that Pseudomonas bacteria grow in a wide range and inhabit soil, fresh water, seawater, plants, or animals.
  • the Pseudomonas bacteria in the present invention are not particularly limited, but those having a plant protecting action are preferably used. Specific examples thereof include Pseudomonas Purotegensu (Pseudomonas protegens), Pseudomonas fluorescens (Pseudomonas fluorescens), Pseudomonas syringae (Pseudomonas syringae), Pseudomonas chlororaphis (Pseudomonas chlororaphis), Pseudomonas Shinki Santa (Pseudomonas synxantha), Pseudomonas brush skein alum (Pseudomonas brassica acerum), Pseudomonas putida (Pseudomonas putida), Pseudomonas rhodesia (Pseudomonas
  • the St29 strain can also be used, and these bacteria are the National Institute of Technology and Evaluation (NITE), Biotechnology Center, Patent Microbiology Deposit Center (NPMD) (Kazusa, Kisarazu City, Chiba Prefecture, Japan) In Kamashita 2-5-8), the deposit numbers NITE P-02053 (date of trust: May 20, 2015) and NITE P-02054 (date of trust: May 20, 2015) were deposited respectively. Yes.
  • St29 strain is used in the present invention.
  • strains of Pseudomonas protegens Pseudomonas protegens CHA0 strain and Pseudomonas protegens Cab57 strain are preferably used, but are not particularly limited thereto.
  • Pseudomonas bacteria may be those that have been isolated by themselves, may be those that have been distributed from a facility, or Pseudomonas bacteria that are commercially available as biopesticidal products (ie, commercially available products). May be used, and the use thereof is not particularly limited.
  • Glutamic acid is a kind of amino acid and is a compound represented by the chemical formula HOOC (CH 2 ) 2 CH (NH 2 ) COOH (or C 5 H 9 NO 4 ). Further, glutamic acid is classified as a hydrophilic amino acid, a polar amino acid, a polar charged amino acid (polar negatively charged amino acid), or an acidic amino acid, and may be represented by an abbreviation of Glu or E. Glutamic acid is contained in plants and animals, and specifically, it is known to be contained in seaweed, flour, soybeans, sugar cane and the like.
  • glutamic acid can be any of D-form (D-glutamic acid), L-form (L-glutamic acid), and DL-form (DL-glutamic acid), and is not particularly limited.
  • L-form glutamic acid (L-glutamic acid) is preferred.
  • the CAS number of D-form glutamic acid is 6893-26-1 and the CAS number of L-form glutamic acid is 56-86-0.
  • glutamic acid may be a salt.
  • the salt of glutamic acid include alkali metal salts such as sodium and potassium (specifically, sodium glutamate and potassium glutamate), and alkaline earth metal salts such as calcium and magnesium (specifically, calcium glutamate and glutamic acid). Magnesium), zinc glutamate, and iron glutamate.
  • the method for obtaining glutamic acid is not particularly limited, and may be any natural product isolated and purified from animals or plants, or one obtained by chemical synthesis or fermentation.
  • glutamic acid one purified by itself or a commercially available product may be used, and the use thereof is not particularly limited.
  • commercially available glutamic acid is preferably used.
  • soil in this specification means soil in which plants can grow.
  • the soil used in the present invention may be indicated by a name such as cultivated soil, cultured soil, nursery soil, or nursery soil. Further, the soil of Yamano that has not been subjected to any processing may be used as it is.
  • the particle size of the soil is not particularly limited, and may be any soil as long as the plant can grow.
  • the soil to be applied in the present invention is preferably soil in the vicinity of the plant (for example, soil in a range within 10 cm from the plant).
  • the plant in the present invention is not particularly limited, but is preferably an agricultural product.
  • agricultural products include, but are not limited to, vegetables, cereals, fruits, flowers, beans, and the like. Specific examples thereof include cucumbers (cucumbers, watermelons, pumpkins, zucchini, gourds, loofahs, tougans, teppouri, yugao, tsurureishi (nigauri, bitter gourd), melons, etc.), potatoes (potatoes, sweet potatoes, taros, potatoes) , Yam, etc.), root vegetables (cubs, Japanese radish, Japanese radish, horseradish, horseradish, burdock, chorogi, ginger, carrots, sea bream, lotus root, lily root, etc., leafy vegetables (green mustard, cabbage, watercress, kale) Komatsuna, Saishin, Sanchu, Shandong Vegetable, Sengoku, Sirona, Celery, Celery, Taasai
  • the state of the plant to be granted in the present invention is not particularly limited, and may be a seed or a seedling or a state in which a plant has already grown, but is preferably a seed or a seedling, and more preferably a seed.
  • a seed or a seedling By being in the early stages of growth such as seeds and seedlings, it is possible to suppress the beginning of morbidity of soil infectious diseases, and it is possible to more effectively control soil infectious diseases in plants.
  • the plant tissue is young, Pseudomonas bacteria and glutamic acid easily penetrate into the plant, and in this respect, it is possible to more effectively control soil infectious diseases.
  • the plant is particularly preferably a rooted seed.
  • the Pseudomonas bacterium and glutamic acid can be imparted to any part of the plant such as root, leaf, stem, branch, or trunk.
  • the aforementioned Pseudomonas bacteria and glutamic acid are imparted to soil or plants, but the method and means relating to the impartation are not particularly limited as long as the effects of the present invention can be obtained.
  • the application operation may be performed by preparing water (aqueous solution) suspended or dissolved together with Pseudomonas bacteria and glutamic acid, or separately, and bringing the prepared water (aqueous solution) into contact with soil or a plant. it can.
  • the form of Pseudomonas bacteria and glutamic acid may be liquid or solid.
  • pseudomonas genus bacteria and glutamic acid can be provided to soil or a plant by operation, such as spraying, dripping, or immersion.
  • a container may be prepared separately, and a holed container containing soil or a plant may be put into the container containing the liquid to perform the operation.
  • the form of Pseudomonas bacteria and glutamic acid is solid, it may be applied by placing the solid on or in the soil, or by contacting the solid with the surface of the plant body. May be.
  • the amounts of Pseudomonas bacteria and glutamic acid to be applied to the soil or the plant are not particularly limited, and can be appropriately set according to the application form, the type of target plant, and the like.
  • Pseudomonas bacteria when they are applied to soil, they can be applied to 1 cm 3 of soil at a bacterial count of 10 4 to 10 20 CFU, preferably 10 6 to 10 10 CFU per time.
  • glutamic acid when glutamic acid is applied to soil, it can be applied in an amount of 0.01 mM to 1 M, preferably 0.1 mM to 100 mM per 1 cm 3 of soil.
  • the application amount of Pseudomonas bacteria and glutamic acid can be adjusted by the concentration in the preparation containing them and the application amount of the preparation.
  • Pseudomonas bacteria when imparted to a plant, it can be imparted to each individual plant at a bacterial count of 10 4 to 10 20 CFU, preferably 10 6 to 10 10 CFU. .
  • glutamic acid when glutamic acid is added to a plant, it can be applied to an individual plant in an amount of 0.01 mM to 1 M, preferably 0.1 mM to 100 mM per time.
  • the application amount of Pseudomonas bacteria and glutamic acid can be adjusted by the concentration in the preparation containing them and the application amount of the preparation.
  • glutamic acid is a salt, the amount of glutamic acid can be calculated from the molecular weight corresponding to the free form (glutamic acid ion).
  • Pseudomonas bacteria and glutamic acid may be applied before, during or after planting (including seedling and planting periods), but is not particularly limited. Within 3 days before or after sowing.
  • seeding in the present specification means that a plant is planted in soil as a starting point of growth, and includes, for example, sowing seeds in soil and planting seedlings in soil.
  • the specific timing is not particularly limited. For example, within 7 days before sowing, within 6 days before sowing, within 5 days before sowing, and 4 days before sowing. Within 3 days before sowing, within 2 days before sowing, within 1 day before sowing, within 12 hours before sowing, within 6 hours before sowing, within 3 hours before sowing, within 1 hour before sowing, within 30 minutes before sowing, Within 10 minutes before sowing, within 5 minutes before sowing, within 1 minute before sowing, or within 30 seconds before sowing.
  • the granting time may be within 7 days after sowing, within 6 days after sowing, within 5 days after sowing, or within 4 days after sowing, or within 2 days after sowing.
  • Within 1 day after sowing within 12 hours after sowing, within 6 hours after sowing, within 3 hours after sowing, within 1 hour after sowing, within 30 minutes after sowing, within 10 minutes after sowing, within 5 minutes after sowing, sowing It may be within 1 minute after, or within 30 seconds after sowing.
  • the application time is typically within 7 days before and after sowing.
  • Pseudomonas bacteria and glutamic acid may be added simultaneously or separately. Although it does not specifically limit in the method of this invention, after giving any one among Pseudomonas bacteria and glutamic acid, the other can be provided within the 3rd.
  • the time interval between the two is within 2 days, within 1 day, within 12 hours, within 6 hours, within 3 hours, within 1 hour, within 30 minutes, within 10 minutes, within 5 minutes, within 1 minute, or 30 It may be within seconds.
  • glutamic acid is considered to have a function of dramatically enhancing the soil infectious disease control action of Pseudomonas bacteria, and therefore it is preferable that the interval between the two application times does not become long.
  • the interval between the application times in the case of applying Pseudomonas bacteria and glutamic acid is at least one day or less.
  • inoculation of the plant mentioned above is applied as a time when any one is provided, and the other can be provided within 3 days after the application.
  • Pseudomonas bacteria or glutamic acid may be added first. That is, Pseudomonas bacteria may be applied first, followed by glutamic acid, or glutamic acid may be applied first, followed by Pseudomonas bacteria. In the present invention, although not particularly limited, it is preferable to give Pseudomonas bacteria first and then give glutamic acid. If Pseudomonas spp. Is applied first, the adhesion of Pseudomonas spp. To the surface of the plant (eg, seed) can be enhanced, thereby more effectively controlling soil infectious diseases in the plant. It is considered possible.
  • the method of the present invention can also include a step of further adding glutamic acid after adding Pseudomonas bacteria and glutamic acid. It is expected that further enhancement of glutamic acid can sustain the effect of controlling Pseudomonas bacteria in controlling soil-borne diseases, and it is expected that soil-borne diseases can be controlled more effectively. Further application of glutamic acid can be performed once or more, and may be performed twice or more, three times or more, four times or more, or five times or more. Further application of glutamic acid is typically 1 to 20 times, preferably 2 to 10 times, more preferably 3 to 8 times.
  • the time interval for further applying glutamic acid is not particularly limited, and can be, for example, 6 hours or more, 12 hours or more, 1 day or more, 2 days or more, 3 days or more, or 4 days or more. Or less, 7 days or less, 6 days or less, 5 days or less, 4 days or less, 3 days or less, 2 days or less, 1 day or less, or 12 hours or less.
  • the time interval for further application of glutamic acid is typically 6 hours to 10 days, preferably 12 hours to 7 days, more preferably 1 to 3 days.
  • the form and means for further adding glutamic acid are not particularly limited, and can be the same as described above.
  • the amount of glutamic acid to be further added is not particularly limited, and can be the same as described above.
  • the soil infectious disease targeted in the present invention is not particularly limited as long as it is a disease caused by pathogenic microorganisms that survive in the soil.
  • pathogenic microorganisms that survive in the soil include filamentous fungi (fungi, oomycetes, etc.).
  • filamentous fungi include the genus Pythium (Pythium ultimum), Pythium aphanidermatum, Pythium megacalumumum, Pythium megagalumumum, etc.
  • Genus fungus (Fusarium oxysporum), Fusarium graminearum, Fusarium solani, etc., Rhizotonia opiumi sis) spp., and the like.
  • pathogenic microorganisms other than filamentous fungi, Erwinia genus bacteria (Erwinia carotovora), Ralstonia genus bacteria (Ralstonia solanaacearum), Pectinobacter Pecto (Pectobacterium carotovorum, etc.), Burkholderia (Burkholderia glumae, etc.), Agrobacterium (Agrobacterium) genus Agrobacterium (Agrobacterium) Pathogenic microorganisms in. The present invention Faciens) etc.) and the like, it is preferable to target fungi.
  • soil infectious diseases include seedling blight, psium rot, bed replacement root rot, wilt, leaf rot, root rot, vine split disease, blight, yellow rot, buttocks rot , Root rot debilitating disease, cone browning disease, rot disease, leaf blight disease, dry rot disease, plant blight disease, red mold disease, bulb rot disease, stem rot wilt disease, stem rot disease, root rot wilt disease, black stripe Real rot, half blight, black spot, panama disease, brown rot, fusarium disease, strain rot, coat blight, bud blight, spider web disease, brown blight, underbrush disease, rhizoctonia disease , Corm rot, black bruise, perforated leaf blight, dry root rot, large white silk disease, tiger spot disease, lie rot, real rot, hip fold disease, forest root rot, buttocks rot Skin rot, brown bruise, brown
  • composition for controlling soil infectious diseases of plants is a composition for controlling soil infectious diseases of plants, which comprises Pseudomonas bacteria and glutamic acid.
  • the Pseudomonas bacterium and glutamic acid used in the composition of the present invention are as described above.
  • the amount of Pseudomonas bacteria contained in the composition of the present invention can be appropriately set according to the bacterial species, the nature of the bacteria (such as drought resistance), the type of plant to be applied, the type of dosage form, and the like.
  • the content of Pseudomonas bacteria in the composition of the present invention is, for example, 10 4 to 10 20 CFU, preferably 10 6 to 10 12 CFU per 100 g of the composition, but is not particularly limited.
  • the method for adding Pseudomonas bacteria to the composition of the present invention is not particularly limited, and may be added as it is.
  • Pseudomonas bacteria are cultivated by a method known to those skilled in the art, and if a liquid medium is collected by centrifugation or the like, if it is a solid medium, formed colonies are collected with a platinum loop or the like to obtain the composition of the present invention. Can be added.
  • lyophilization may be performed by a method known per se from the state stored in a liquid, and Pseudomonas bacteria may be added to the composition of the present invention as a solid substance.
  • the amount of glutamic acid contained in the composition of the present invention can be appropriately set according to the type of plant to be applied and the type of dosage form.
  • the glutamic acid content in the composition of the present invention is, for example, 0.01 mM to 1 M, preferably 0.1 mM to 100 mM per 100 g of the composition, but is not particularly limited.
  • the composition of the present invention may contain additives such as excipients, thickeners, binders, stabilizers, preservatives, pH adjusters, colorants, and flavoring agents.
  • additives are not particularly limited, and materials known in the technical field of biopesticides can be used, and the amount of the additives can be appropriately adjusted based on the known techniques of those skilled in the art.
  • the form of the composition of the present invention may be any form such as liquid, solid, gel, paste, and the like, and can be appropriately set according to the usage situation and the like.
  • the composition of the present invention may be in the form of a concentrate.
  • the concentration factor is not particularly limited, and can be, for example, 2 to 1000 times, 5 to 100 times, or 10 to 50 times.
  • the composition of this invention can dilute suitably using solvents, such as water, and the dilution can be provided to soil or a plant.
  • the content of Pseudomonas bacteria and glutamic acid in the composition of the present invention can be set according to the concentration factor.
  • composition of the present invention may also be configured as a single preparation containing Pseudomonas bacteria and glutamic acid, or may be configured as two preparations containing Pseudomonas bacteria and glutamic acid separately. Good.
  • composition of the present invention is constituted as two preparations, it is preferable that the two preparations are used in combination (that is, a combination preparation).
  • the composition of the present invention is configured as two preparations, the two preparations may be different types of dosage forms or the same type of dosage form.
  • the composition of the present invention is used for controlling soil infectious diseases of plants.
  • the target plant and soil infectious disease are not particularly limited, and specific examples thereof are the same as those described above.
  • the composition of the present invention can also be used as a biopesticide or a microbial pesticide. Therefore, the composition of the present invention can also be referred to as an agrochemical composition.
  • Plant soil-borne disease control kit One embodiment of the present invention is a plant soil-borne disease control kit containing Pseudomonas bacteria and glutamic acid.
  • the Pseudomonas bacteria and glutamic acid used in the kit of the present invention are as described above.
  • the Pseudomonas bacterium and glutamic acid in the kit of the present invention may be a reagent or a preparation, and the form thereof is not particularly limited. Moreover, both Pseudomonas bacteria and glutamic acid may be contained in the same formulation, or may be separately contained in separate formulations. When Pseudomonas bacteria and glutamic acid are divided into separate preparations, the preparations may be in different types or in the same type.
  • the form of the preparation used in the kit of the present invention is not particularly limited, and may be any form such as a liquid, a solid, a gel, and a paste.
  • the contents of Pseudomonas bacteria and glutamic acid in the preparation are not particularly limited, and can be arbitrarily set according to the above-described composition of the present invention.
  • the preparation used in the kit of the present invention may be individually packaged for single use as a single use, or multiple times (for example, 2, 3, 4, 5, 10 times, (Or more) may be packaged in a form including the amount used.
  • the container to be used is not particularly limited, and can be appropriately set according to the amount of the preparation used.
  • kits of the present invention When the Pseudomonas bacterium and glutamic acid are separated into separate reagents or preparations in the kit of the present invention, both do not need to be incorporated in the same package, and are packaged separately and used together at the time of use It may be. Moreover, the kit of this invention may contain the instruction manual regarding use of Pseudomonas genus bacteria and glutamic acid.
  • the kit of the present invention is used for controlling soil infectious diseases of plants.
  • the target plant and soil infectious disease are not particularly limited, and specific examples thereof are the same as those described above.
  • the kit of the present invention can also be used as a biopesticide or a microbial pesticide.
  • Cucumber seeds were arranged on a filter paper soaked with sterilized water and placed in the dark at 26 ° C. for 24 hours.
  • Pseudomonas genus bacterium Pseudomonas protogens CHA0 strain was used, and cultured with shaking in a NYB medium (Nutrient broth: 25 g, Yeast extract: 5 g, H 2 O: 1000 ml) at 30 ° C., 180 rpm for 24 hours.
  • the prepared vermiculite was equally divided into 50 mL each in a seedling raising tray (5 cm square per section, depth 5 cm) having a hole at the bottom and allowing water to permeate. Two cucumber seeds with rooting confirmed were spread on vermiculite.
  • Pseudomonas bacteria and glutamic acid were added to cucumber seeds and the nearby soil. Specifically, 4 mL of an adjusted aqueous solution of Pseudomonas bacteria was added to each section of the seedling raising tray toward the cucumber seeds, and vermiculite that had been separated without infecting Psium bacteria was added Each of the seedling trays was covered with 15 mL from the top, and the seedling tray was immersed in water in a vat or a 10 mM glutamic acid (L-glutamic acid) aqueous solution (10 mL for each section of the seedling tray). The application of Pseudomonas bacteria and glutamic acid was performed within 2 hours after sowing of cucumber seeds, and the interval between the application periods of Pseudomonas bacteria and glutamic acid was within 1 hour.
  • the seedling tray was placed in a plant incubator set at 25 ° C.
  • the light period was set to 16 hours and the dark period was set to 8 hours.
  • the cultivation period in the incubator was 2 weeks, and water in the vat or 10 mM glutamic acid aqueous solution was replaced every other day.
  • FIG. 1 The results of this experimental example are shown in FIG. As shown in the photograph of FIG. 1, it was revealed that the effect of controlling soil infectious diseases is enhanced by using Pseudomonas bacteria together with glutamic acid. In addition, since glutamic acid itself did not seem to have a high soil infectious disease control effect, glutamic acid acts on Pseudomonas bacteria and has the effect of enhancing the soil infectious disease control action of Pseudomonas bacteria. It was thought that there was.
  • a 10 mM histidine (L-histidine) aqueous solution was prepared and used instead of the glutamic acid aqueous solution in Experimental Example 1 above.
  • the vermiculite adhering to the root of the cucumber was thoroughly washed away.
  • Rhizoctonia genus Rhizotonia solani MAFF726551 strain
  • Pseudomonas protegens Cab57 strain was used as a Pseudomonas genus bacterium.
  • the technology provided by the present invention is useful in the agricultural field from the viewpoint of effectively controlling agricultural products as a plant from soil-borne diseases.
  • the Pseudomonas bacterium and glutamic acid provided by the present invention can be used in the field of agricultural chemicals, in particular, in the field of biological agricultural chemicals.

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