WO2019082261A1 - Conteneur de cellules - Google Patents

Conteneur de cellules

Info

Publication number
WO2019082261A1
WO2019082261A1 PCT/JP2017/038304 JP2017038304W WO2019082261A1 WO 2019082261 A1 WO2019082261 A1 WO 2019082261A1 JP 2017038304 W JP2017038304 W JP 2017038304W WO 2019082261 A1 WO2019082261 A1 WO 2019082261A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
container body
lid member
lid
flange
Prior art date
Application number
PCT/JP2017/038304
Other languages
English (en)
Japanese (ja)
Inventor
学司 加藤
順一 桑原
Original Assignee
株式会社サンプラテック
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社サンプラテック filed Critical 株式会社サンプラテック
Priority to JP2019549707A priority Critical patent/JP7064247B2/ja
Priority to PCT/JP2017/038304 priority patent/WO2019082261A1/fr
Publication of WO2019082261A1 publication Critical patent/WO2019082261A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus

Definitions

  • the present invention relates to a cell storage container used to store and culture cells and living tissues in a liquid-sealed state, and more particularly to a cell storage container suitable for transporting cells and living tissues while maintaining the culture state. .
  • culture vessels such as dishes (dishes), well plates, probes and the like are widely used. These culture vessels were designed for the purpose of culturing in a ventilated state, and even if there was a lid, it could not be sealed only by mounting.
  • affixing a film with an adhesive to the upper edge of the cylindrical wall of the vessel is generally performed.
  • the method of covering the upper edge part of the cylindrical wall of a container with an elastomer sheet is proposed (for example, refer patent document 1).
  • freeze transport it is necessary to transfer cultured cells from the culture vessel to a dedicated container for cryopreservation, freeze them, transport them, and then thaw them and transfer them again to the culture vessel containing the necessary amount of medium.
  • frozen transport there is a risk of cell loss and contamination due to thawing and transfer of containers, and there is a disadvantage that it takes a lot of time and technology to make the cells available.
  • the present invention was conceived under such circumstances, and has as its main object to provide a cell storage container suitable for maintaining and transporting a cultured state.
  • the present invention adopts the following technical means.
  • a cell storage container provided by the present invention has a container body having an opening at one side end in a first direction and having a first tubular portion extending in the first direction, and a lid capable of closing the opening. And the lid covers the second tubular portion fitted to the first tubular portion, the annular portion, and a relatively thick flange, and closes the inside of the flange, and A relatively thin thin portion, the flange and the thin portion being made of a flexible material, and the flange being overlapped with the peripheral edge portion of the opening in the container body in the first direction view And locking means for blocking relative movement between the first tubular portion and the second tubular portion.
  • the thin portion has gas permeability.
  • the thin portion is transparent.
  • the lid is an annular top plate, and extends from the outer peripheral edge of the top plate in the thickness direction of the top plate, and the lid is externally fitted to the first tubular portion.
  • a second lid member made of a flexible material having the flange and the thin-walled portion, the second lid member including at least at least the flange.
  • the locking means is provided in at least one of the container body and the first lid member, and the peripheral portion of the opening in the container body and the first lid. The flange between the top plate portion of the member In conditions mediated preventing relative movement between said first cylindrical portion and the second cylindrical portion.
  • the second lid member is accommodated inside the first cylindrical portion of the container body at a distance from the first cylindrical portion, and is directed to the other side of the first direction.
  • the protruding portion includes the thin portion.
  • the thin-walled portion can be displaced following changes in the internal pressure of the container.
  • the first lid member has a port having one end at one end in the first direction open to the outside and the other end at the other end in the first direction open in the inner space of the container body. It is provided.
  • a cap is provided which can close one end of the port in the first direction.
  • the second lid member has a port having one end at one end in the first direction open to the outside and the other end at the other end in the first direction open in the inner space of the container body. It is provided.
  • the locking means comprises an external thread provided on one of the first and second cylindrical portions, and the first and second cylindrical portions. And a female screw provided on the other of the parts and capable of being screwed to the male screw.
  • the locking means comprises a hinge mechanism which can be switched between the closing of the opening and the opening of the opening.
  • a bottom wall inside the container body, a bottom wall, a cylindrical side wall rising from the bottom wall, and an annular outward flange extending radially outward from an edge of the side wall, And a flexible container member made of a flexible material separate from the container body is disposed, and the outward flange is a peripheral portion of the opening in the container body and the above-mentioned second lid member. Intervenes with the flange.
  • the bottom wall is provided with one or more first protrusions that protrude toward one side in the first direction.
  • the container body has a bottom portion connected to the other side end of the first cylindrical portion in the first direction or in the vicinity thereof and closing the inside of the first cylindrical portion,
  • the bottom portion is provided with one or more second protrusions that project toward one side in the first direction.
  • the container body has a bottom portion connected to the other side end of the first cylindrical portion in the first direction or in the vicinity thereof and closing the inside of the first cylindrical portion, At the bottom portion, a supporting member made of a flexible material separate from the container body is placed, and the supporting member is one or more projecting toward one side in the first direction. It has a third protrusion.
  • the second lid member has one or more fourth projections that project to the other side in the first direction on the inner side of the container body.
  • the top plate portion of the first lid member has a convex portion protruding to one side in the first direction, and a tip end of the convex portion is an end face of the top plate portion
  • the projections are substantially parallel, and the convex portions do not form a closed ring in the thickness direction of the top plate.
  • the second cylindrical portion extends from the inner peripheral edge of the flange in the thickness direction of the flange and is internally fitted to the first cylindrical portion, and the lid is flexible. It is integrally formed of a material having elasticity.
  • the outer diameter of the second cylindrical portion in the natural state is larger than the inner diameter of the first cylindrical portion, and the locking means is the first cylindrical portion.
  • the second cylindrical portion is press-fitted to the
  • the lid has a convex portion projecting from the end face of the flange toward one side in the first direction, and the tip of the convex portion is substantially parallel to the end face of the flange.
  • the convex portion does not form a closed annular shape in the thickness direction of the flange.
  • FIG. 1 is a perspective view showing a first embodiment of a cell storage container according to the present invention. It is a disassembled perspective view of the cell accommodating container shown in FIG. It is an enlarged longitudinal cross-sectional view of the cell accommodation container shown in FIG. It is sectional drawing similar to FIG. 3, and shows the state which accommodated the content in the container body (soft container member). It is a perspective view which shows an example of the member for mounting a cultured cell. It is an expansion longitudinal cross-sectional view which shows the modification of the cell accommodation container shown in FIG. It is an expansion longitudinal cross-sectional view which shows the modification of the cell accommodation container shown in FIG. It is sectional drawing similar to FIG. 7, and shows the state which accommodated the contents in the container body.
  • FIG. 17 is a plan view of the cell container shown in FIG. It is a longitudinal cross-sectional view of the cell accommodation container shown in FIG. It is a cross-sectional view similar to FIG. 18, and shows a state in which the contents are accommodated in the container body (soft container member).
  • FIG. 21 is an exploded perspective view of the cell container shown in FIG. 20.
  • FIG. 23 is a cross-sectional view taken along the line XXIII-XXIII of FIG. 22.
  • FIG. 23 is a cross-sectional view similar to FIG. 22, showing a state in which the contents are accommodated in a container body (soft container member).
  • FIG. 29 It is a perspective view which shows 2nd Embodiment of the cell storage container which concerns on this invention. It is a longitudinal cross-sectional view of the cell accommodation container shown in FIG. It is a perspective view which shows the modification of the cell accommodating container shown in FIG. It is a longitudinal cross-sectional view of the cell accommodation container shown in FIG. It is a perspective view which shows 3rd Embodiment of the cell storage container which concerns on this invention. It is a longitudinal cross-sectional view of the cell accommodation container shown in FIG. In the cell container shown in FIG. 29, it is a perspective view which shows the state which the opening of a container body opens. It is a longitudinal cross-sectional view of the cell accommodation container shown in FIG. It is a perspective view which shows 4th Embodiment of the cell storage container which concerns on this invention. It is a longitudinal cross-sectional view of the cell accommodation container shown in FIG.
  • FIGS. 1 and 3 show a first embodiment of a cell storage container according to the present invention.
  • the cell storage container A10 of the present embodiment includes a container body 1, a lid 2, and a soft container member 3. Although the details will be described later, FIGS. 1 and 3 show an assembled state in which the container body 1, the lid 2 and the flexible container member 3 are combined.
  • FIG. 2 is an exploded perspective view of components of the cell storage container A10.
  • the container body 1 includes the first cylindrical portion 11 and the bottom portion 12 and has a container shape having an opening 110 at the upper end (first direction one side end) of the first cylindrical portion 11. .
  • the first cylindrical portion 11 has a substantially cylindrical shape, and a male screw 111 is formed on the upper outer peripheral surface of the first cylindrical portion 11.
  • the bottom portion 12 is connected to the vicinity of the lower end of the first cylindrical portion 11 (near the other side end in the first direction), and closes the inside of the first cylindrical portion 11.
  • a slit 112 is formed at an appropriate position of the lower end of the first cylindrical portion 11.
  • an extension piece 113 is provided at an appropriate position of the lower end of the first cylindrical portion 11.
  • the extension piece 113 is a portion connected to the first tubular portion 11 and extending radially outward.
  • the extension piece 113 is provided at a position different from the slit 112 in the circumferential direction of the first tubular portion 11.
  • the lid 2 includes a first lid member 2A and a second lid member 2B, and is for closing the opening 110 of the container body 1 and sealing the container body 1 (soft container member 3 described later in this embodiment). is there.
  • the first lid member 2A has a top plate portion 21 and a second cylindrical portion 22.
  • the top plate portion 21 has a substantially annular shape, and between the flange 23 of the second lid member 2B described later and the upper end portion 114 (the peripheral edge portion of the upper end opening) of the first cylindrical portion 11 of the container 1 It is a part to pinch.
  • a through hole 211 is formed on the inner side in the radial direction of the top plate portion 21. The through hole 211 is surrounded by the top plate 21 in the thickness direction of the top plate 21.
  • the second cylindrical portion 22 extends from the peripheral edge of the top plate 21 in the thickness direction (downward in the drawing) of the top plate 21 and is substantially cylindrical.
  • a female screw 221 is formed on the inner peripheral surface of the second cylindrical portion 22, and the female screw 221 can be screwed to the male screw 111 of the container body 1 (first cylindrical portion 11).
  • the container body 1 and the first lid member 2A are made of, for example, a translucent or transparent plastic material.
  • the plastic material for example, besides polystyrene and methylpentene, materials having transparency such as polycarbonate, cycloolefin polymer, cycloolefin copolymer and the like are preferably used, but the material is not limited thereto.
  • the second lid member 2B is, for example, a rubber molded product, and as shown in FIG. 3, the flange 23, the inclined portion 24, the central thick portion 25 (projecting portion), the thin portion 26, and the plurality of fourth portions. And a protrusion 27.
  • the flange 23 has a substantially annular shape, and overlaps with the top plate 21 in the thickness direction of the top plate 21.
  • the flange 23 has an appropriate thickness and has an appropriate elastic restoring force with respect to a load in the vertical direction (first direction).
  • the thickness of the flange 23 is, for example, about 1 to 3 mm.
  • the flange 23 is interposed between the peripheral edge portion (the upper end portion 114 of the first cylindrical portion 11) of the opening 110 in the container body 1 and the top plate portion 21 in the first lid member 2A. ing.
  • the inclined portion 24 is connected to the inner peripheral edge of the flange 23 and extends radially inward.
  • the inclined portion 24 is inclined to be displaced to the lower side (the other side in the first direction) as it goes radially inward.
  • the thickness of the inclined portion 24 is substantially the same as the thickness of the flange 23.
  • the central thick portion 25 is connected to the inner peripheral edge of the inclined portion 24 and extends radially inward, and has a substantially annular shape.
  • the central thick portion 25 is accommodated inside the first cylindrical portion 11 of the container 1 at a distance from the first cylindrical portion 11 and protrudes downward (the other side in the first direction).
  • the central thick portion 25 corresponds to the projecting portion in the present invention.
  • the thickness of the central thick portion 25 is approximately the same as the thickness of the flange 23.
  • the thin portion 26 is provided at the center of the second lid member 2B.
  • the thin portion 26 closes the radially inner side of the central thick portion 25 (flange 23).
  • the thin portion 26 is a portion whose thickness is thinner than other portions.
  • the thin portion 26 has a substantially circular shape in the vertical direction (first direction).
  • the thickness of the thin portion 26 is, for example, about 0.2 to 0.3 mm.
  • the thin portion 26 has gas permeability.
  • the thin portion 26 is transparent. The thin portion 26 overlaps the through hole 211 surrounded by the top plate 21 in the thickness direction of the top plate 21.
  • the plurality of fourth protrusions 27 project downward from the lower surface of the central thick portion 25 (the other side in the first direction). As understood from FIG. 3, the fourth protrusions 27 are radially provided in the vertical direction (first direction).
  • the second lid member 2B is made of a flexible and elastic material.
  • a material which constitutes the 2nd lid member 2B silicone rubber, natural rubber, urethane rubber, elastomer resin, etc. are mentioned, for example.
  • the material of the second lid member 2B is non-cytotoxic and is a biocompatible silicone rubber for medical use. Is more preferred.
  • the hardness of the second lid member 2B it is preferable that, for example, the rubber hardness is approximately 20 degrees to 40 degrees.
  • the outer diameter of the flange 23 of the second lid 2B is slightly larger than the inner diameter of the second cylindrical portion 22 of the first lid 2A in the natural state.
  • the second lid member 2B is held by the first lid member 2A by being fitted inside the second cylindrical portion 22 while the flange 23 is elastically compressed.
  • the soft container member 3 is for containing cultured cells and a culture medium.
  • the soft container member 3 is made of a flexible and elastic material.
  • a raw material which comprises the soft container member 3 silicone rubber, natural rubber, urethane rubber, an elastomer resin etc. are mentioned, for example.
  • the material of the soft container member 3 is a medical silicone rubber having no cytotoxicity and having biocompatibility. Is more preferred.
  • the hardness of the soft container member 3 is preferably, for example, about 20 degrees to 40 degrees of rubber hardness.
  • the soft container member 3 is, for example, a rubber molded product, and has a bottom wall 31, a cylindrical side wall 32 rising from the bottom wall 31, and an outward flange 33 as shown in FIGS. 2 and 3.
  • the bottom wall 31 has a substantially circular shape in plan view.
  • the side wall 32 has a cylindrical side wall 321 having a substantially cylindrical shape and an inclined side wall 322.
  • the cylindrical side wall portion 321 extends upward (one side in the first direction) from the outer peripheral edge of the bottom wall 31.
  • the inclined side wall portion 322 is connected to the upper end of the cylindrical side wall portion 321 and extends radially outward.
  • the inclined side wall portion 322 is inclined so as to be displaced upward (one side in the first direction) as it goes radially outward.
  • the sloped side wall portion 322 and the sloped portion 24 of the second lid member 2B have a substantially constant interval.
  • the outwardly directed flange 33 is generally annular and extends radially outward from the upper end edge of the sloped sidewall 322 (sidewall 32).
  • the outward flange 33 has an appropriate thickness, and has an appropriate elastic restoring force with respect to the load in the vertical direction (first direction).
  • the thickness of the outward flange 33 is, for example, about 1 to 3 mm.
  • the outward flange 33 is interposed between the peripheral edge (the upper end 114 of the first cylindrical portion 11) of the opening 110 in the container body 1 and the flange 23 in the second lid member 2B. ing.
  • the side wall 32 is inserted into the opening 110 of the container body 1, and the side wall 32 and the bottom wall 31 are inserted into the first tubular portion 11 of the container body 1.
  • An annular protrusion 34 is provided on the lower surface of the outward flange 33.
  • the outer diameter dimension of the annular protrusion 34 is substantially the same as or slightly larger than the inner diameter dimension of the first cylindrical portion 11.
  • the annular projection 34 functions as positioning when the soft container member 3 is set inside the container body 1.
  • the bottom wall 31 is provided with a plurality of first protrusions 35.
  • the first protrusions 35 protrude upward (one side in the first direction) from the bottom wall 31.
  • the first protrusions 35 are radially provided in the vertical direction (first direction).
  • the plurality of first protrusions 35 are provided at positions corresponding to the fourth protrusions 27 in the second lid member 2B.
  • the container member thin portion 311 is provided at the center of the bottom wall 31. As well shown in FIG. 4, the container member thin portion 311 is a portion whose thickness is thinner than other portions.
  • the container member thin portion 311 has a substantially circular shape in the vertical direction (first direction).
  • the thickness of the container member thin portion 311 is, for example, about 0.2 to 0.3 mm.
  • the thin container portion 311 is transparent.
  • the cell storage container A10 stores the cultured cells, the living tissue and the culture medium in the container body 1 (especially in the present embodiment, the soft container member 3), hermetically stores it while maintaining the culture state, and transports it (culture state maintenance transport) Used to The cultured cells, the living tissue and the culture medium contained in the container body 1 (soft container member 3) are not particularly limited.
  • the cell preparation used for regenerative medicine may be in the form of a suspension or in the form of a cell sheet laminated on a support.
  • the medium When transporting a non-frozen cell suspension or cell sheet, it is necessary to fill the entire inside of the soft container member 3 with the medium to suppress the movement of the medium in order to prevent the cells from being damaged by the medium being shaken. .
  • FIG. 4 shows the assembled state of the cell storage container A10 when the inside of the soft container member 3 is filled with the culture medium M1.
  • the lid 2 is configured to include the first lid member 2A and the second lid member 2B.
  • the second lid member 2B is formed of a flexible material and is held by the first lid member 2A. Then, by tightening the female screw 221 of the first lid member 2A (second tubular portion 22) into the male screw 111 of the container body 1 (first tubular portion 11), the second lid member 2B can be made thicker A certain flange 23 is pinched by the peripheral edge of the opening 110 (upper end 114 of the first cylindrical portion 11) in the container body 1 and the top plate 21 of the first lid member 2A, and the assembly state is maintained.
  • the second lid member 2B is in the form of a series of films ranging from the flange 23 on the outer peripheral side to the thin portion 26 at the center.
  • the external thread 111 of the container body 1 (first tubular portion 11) and the internal thread 221 of the first lid member 2A (second tubular portion 22) constitute the locking means in the present invention.
  • the contents of the soft container member 3 can be appropriately liquid sealed by the cooperation of the container body 1, the first lid member 2A and the second lid member 2B.
  • the thin portion 26 of the second lid member 2B closes the inside of the flange 23.
  • the thin portion 26 overlaps at least a part of the through hole 211 surrounded by the top plate portion 21 in the thickness direction of the top plate portion 21 of the first lid member 2A.
  • the flexible thin portion 26 can be displaced following the change in the internal pressure of the container. Therefore, when the entire inside of the soft container member 3 is filled with the culture medium and sealed by the lid 2, if the culture medium is received to the extent that the soft container member 3 overflows and the lid 2 is tightened, the thin portion 26 absorbs the internal pressure change.
  • the internal volume is displaced so as to slightly increase (displacement upward in FIG. 4), and it is possible to suppress air bubbles from being trapped in the flexible container member 3. Therefore, since the whole inside of the soft container member 3 can be filled with the culture medium without leaving a space in the soft container member 3, it is possible to eliminate the waving of contents (such as the culture medium) due to vibration etc. it can. As a result, the cells and living tissues immersed in the medium in the soft container member 3 can be protected from damage and safely maintained in culture and transported.
  • the state near weightlessness is created by rotating a cell storage container 360 degrees vertically and horizontally.
  • the presence of air bubbles in the container may adversely affect the experimental results, but the cell storage container A10 of the present embodiment can be advantageously used in such applications.
  • the thin portion 26 of the second lid member 2B also has gas permeability. For this reason, with respect to the contents of the container body 1 (soft container member 3), the ventilation state with the outside of the container body 1 (soft container member 3) is maintained. Therefore, according to the present embodiment, it is possible to culture the contents of the container body 1 (soft container member 3) in a ventilated state during sealed storage or transport.
  • the thin portion 26 is transparent. According to such a configuration, it is possible to observe the condition in the container body 1 (soft container member 3) from the upper part of the cell storage container A10 through the transparent thin portion 26.
  • a soft container member 3 is interposed between the container body 1 and the lid 2 (second lid member 2B), and the cultured container or the living tissue and the culture medium are accommodated in the soft container member 3. Ru. And since this soft container member 3 has flexibility, even if the cell and biological tissue accommodated in the inside of the soft container member 3 collide directly with the said soft container member 3, it does not damage easily. Therefore, the cell storage container A10 having the soft container member 3 is suitable for culture state maintenance transport.
  • the second lid member 2B has a plurality of fourth protrusions 27.
  • the fourth protrusions 27 project downward (the other side in the first direction) inside the container body 1 (soft container member 3).
  • a plurality of first protrusions 35 are provided on the bottom wall 31 of the soft container member 3, and the first protrusions 35 protrude upward (one side in the first direction).
  • the support 4 is stably mounted between the fourth protrusion 27 on the second lid member 2B side and the first protrusion 35 on the soft container member 3 side. Can be placed.
  • the support 4 has, for example, a frame 41 and a thin film 42.
  • the frame 41 has a ring shape, and the thin film 42 is formed to close the inside of the frame 41.
  • the thin film 42 is a part on which the cultured cells (cell sheet) are placed, and the cultured cells on the thin film 42 are immersed in the medium. Therefore, according to this embodiment, culture of a cell sheet is appropriately performed via the stably arranged support 4.
  • the soft container member 3 when changing the inner volume of the sealed container according to, for example, the cultured cells and the living tissue contained in the sealed container, or the size of the support 4, etc. It is easily coped with by changing the size of 3.
  • the fourth protrusion 27 of the second lid member 2B and the first protrusion 35 of the soft container member 3 are provided.
  • it is set as a structure, when not considering the support body 4, it is good also as a structure which does not comprise said 4th protrusion 27 or the 1st protrusion 35.
  • a thin container member portion 311 is provided at the center of the bottom wall 31 of the flexible container member 3.
  • the container thin part 311 is transparent. If the container body 1 is formed of a transparent material, the condition in the soft container member 3 is measured from the lower portion of the cell storage container A10 through the bottom portion 12 of the container body 1 and the thin container portion 311 of the soft container member 3. It is also possible to observe.
  • the lid 2 (second lid member 2B) has a central thick portion 25 (protrusion), and the central thick portion 25 is a lower portion (first direction) inside the container 1 (soft container member 3). It projects towards the other side). According to such a configuration, the storage space for the culture medium and the like in the soft container member 3 is reduced. Therefore, the amount of use of the culture medium M1 can be reduced at the time of culture state maintenance transport.
  • the lid 2 After transporting the cell storage container A10, the lid 2 can be removed from the container 1 by loosening the screw locking portions of the container 1 and the lid 2, and the upper portion of the container 1 (soft container member 3) It can be opened. Therefore, unlike the case where the container opening is closed with an adhesive film or the like, when the lid 2 is removed, the problem that the contents of the container body 1 (soft container member 3) overflows is prevented.
  • the fourth protrusion 27 provided on the second lid member 2B and the first protrusion 35 provided on the soft container member 3 is different from the above-described cell storage container A10. ing.
  • the fourth protrusion 27 and the first protrusion 35 are each a single protrusion in an annular shape.
  • the same function and effect as the above-described cell storage container A10 are exhibited.
  • the cell storage container A12 shown in FIG. 7 does not include the soft container member 3 in the above-described cell storage container A10, and accordingly, the configuration of the container body 1 is different from that of the above-described cell storage container A10.
  • the bottom 12 of the container body 1 is provided with a plurality of second protrusions 13.
  • the second protrusions 13 project upward (one side in the first direction) from the bottom 12.
  • the second protrusions 13 are radially provided in the vertical direction (first direction).
  • the plurality of second protrusions 13 are provided at positions corresponding to the fourth protrusions 27 in the second lid member 2B.
  • FIG. 8 shows the assembled state of the cell storage container A12 when the inside of the container body 1 is filled with the medium M1.
  • the lid 2 is configured to include the first lid member 2A and the second lid member 2B.
  • the second lid member 2B is formed of a flexible material and is held by the first lid member 2A.
  • the second lid member 2B can be made thicker A certain flange 23 is pinched by the peripheral edge of the opening 110 (upper end 114 of the first cylindrical portion 11) in the container body 1 and the top plate 21 of the first lid member 2A, and the assembly state is maintained.
  • the second lid member 2B is in the form of a series of films ranging from the flange 23 on the outer peripheral side to the thin portion 26 at the center. As a result, the contents (cultured cells and culture medium) contained in the container body 1 are liquid-sealed.
  • the contents of the container body 1 can be appropriately liquid-sealed by the cooperation of the container body 1, the first lid member 2A and the second lid member 2B. it can.
  • the thin portion 26 of the second lid member 2B closes the inside of the flange 23.
  • the thin portion 26 overlaps at least a part of the through hole 211 surrounded by the top plate portion 21 in the thickness direction of the top plate portion 21 of the first lid member 2A.
  • the state near weightlessness is created by rotating a cell storage container 360 degrees vertically and horizontally.
  • the experimental results may be adversely affected, but the cell storage container A12 of this modification can be advantageously used in such applications.
  • the thin portion 26 of the second lid member 2B has gas permeability. Therefore, the contents of the container body 1 are maintained in the ventilated state with the outside of the container body 1. Therefore, according to the present modification, it is possible to culture the contents of the container body 1 in a ventilated state at the time of sealed storage or transport.
  • the thin portion 26 is transparent. According to such a configuration, it is possible to observe the condition in the container body 1 from the upper part of the cell storage container A12 through the transparent thin portion 26.
  • the second lid member 2 ⁇ / b> B has a plurality of fourth protrusions 27, and the fourth protrusions 27 project downward (the other side in the first direction) inside the container body 1. Further, a plurality of second protrusions 13 are provided on the bottom portion 12 of the container body 1, and the second protrusions 13 project upward (one side in the first direction). According to such a configuration, as shown in FIG. 8, the support 4 is stably placed between the fourth protrusion 27 on the second lid member 2B side and the second protrusion 13 on the container body 1 side. can do.
  • the support 4 has, for example, a frame 41 and a thin film 42.
  • the frame 41 has a ring shape, and the thin film 42 is formed to close the inside of the frame 41.
  • the thin film 42 is a part on which the cultured cells (cell sheet) are placed, and the cultured cells on the thin film 42 are immersed in the medium. Therefore, according to this modification, culture of a cell sheet is appropriately performed via the stably
  • the lid 2 (second lid member 2B) has a central thick portion 25 (protrusion), and the central thick portion 25 is directed to the lower side (the other side in the first direction) inside the container body 1. It is sticking out. According to such a configuration, the storage space for the culture medium and the like in the container body 1 is reduced. Therefore, the amount of use of the culture medium M1 can be reduced at the time of culture state maintenance transport.
  • the lid 2 After transporting the cell storage container A12, the lid 2 can be removed from the container 1 by loosening the screw locking portions of the container 1 and the lid 2, and the upper portion of the container 1 can be opened. Therefore, unlike the case of closing the container opening with an adhesive film or the like, when the lid 2 is removed, the problem that the contents of the container body 1 overflow is prevented.
  • the soft container member 3 in the above-mentioned cell storage container A10 is not provided, but the support member 5 is provided.
  • the support member 5 is made of, for example, a flexible and elastic material.
  • a raw material which comprises the supporting member 5 silicone rubber, natural rubber, urethane rubber, an elastomer resin etc. are mentioned, for example.
  • the material of the support member 5 is preferably a medical silicone rubber which has no cytotoxicity and is biocompatible.
  • the hardness of the support member 5 is preferably, for example, about 20 degrees to 40 degrees of rubber hardness.
  • the support member 5 is, for example, a rubber molded product, and has an annular bottom plate 51 and a plurality of third protrusions 52 that stand up from the annular bottom plate 51.
  • the annular bottom plate 51 is a plate portion having a substantially annular shape and a constant thickness, and is placed on the bottom portion 12 of the container body 1.
  • the outer diameter dimension of the annular bottom plate 51 is set to be substantially the same as or slightly larger than the inner diameter dimension of the first cylindrical portion 11. Thereby, positioning of the supporting member 5 with respect to the 1st cylindrical part 11 (container body 1) is achieved.
  • the plurality of third protrusions 52 project upward (one side in the first direction) from the annular bottom plate 51. These third protrusions 52 are provided so as to form a partial circular arc shape of two types of concentric circles having different diameter dimensions.
  • the third protrusion 52 has a radial dimension corresponding to that of the fourth protrusion 27 provided radially in the second lid member 2B, and a position at which the third protrusion 52 overlaps the fourth protrusion 27 in the vertical direction (first direction).
  • FIG. 10 shows the assembled state of the cell storage container A13 when the inside of the container body 1 is filled with the medium M1.
  • the lid 2 is configured to include the first lid member 2A and the second lid member 2B.
  • the second lid member 2B is formed of a flexible material and is held by the first lid member 2A.
  • the second lid member 2B can be made thicker A certain flange 23 is pinched by the peripheral edge of the opening 110 (upper end 114 of the first cylindrical portion 11) in the container body 1 and the top plate 21 of the first lid member 2A, and the assembly state is maintained.
  • the second lid member 2B is in the form of a series of films ranging from the flange 23 on the outer peripheral side to the thin portion 26 at the center. As a result, the contents (cultured cells and culture medium) contained in the container body 1 are liquid-sealed.
  • the contents of the container body 1 can be appropriately liquid-sealed by the cooperation of the container body 1, the first cover member 2A and the second cover member 2B. it can.
  • the second lid member 2 ⁇ / b> B has a plurality of fourth protrusions 27, and the fourth protrusions 27 project downward (the other side in the first direction) inside the container body 1.
  • a plurality of third protrusions 52 are provided on the support member 5 placed on the bottom portion 12 of the container body 1, and the third protrusions 52 protrude upward (one side in the first direction).
  • the support 4 is stably placed between the fourth protrusion 27 on the second lid member 2B side and the third protrusion 52 on the support member 5 side. can do.
  • the support 4 has, for example, a frame 41 and a thin film 42.
  • the frame 41 has a ring shape, and the thin film 42 is formed to close the inside of the frame 41.
  • the thin film 42 is a part on which the cultured cells (cell sheet) are placed, and the cultured cells on the thin film 42 are immersed in the medium. Therefore, according to this modification, culture of a cell sheet is appropriately performed via the stably arranged support 4.
  • the soft container member 3 in the above-mentioned cell storage container A10 is not provided, and accordingly, the configuration of the lid 2 (second lid member 2B) is the above-mentioned cell storage container It is different from A10.
  • the second lid member 2B has a flange 23, a cylindrical portion 24 ', a central thick portion 25 (projecting portion), and a thin portion 26. That is, the cell storage container A14 has a cylindrical portion 24 'instead of the inclined portion 24 in the above-described cell storage container A10, and does not have the fourth protrusion 27 in the above-described cell storage container A10.
  • the cylindrical portion 24 ' is connected to the inner peripheral edge of the flange 23 and extends downward (the other side in the first direction).
  • the thickness of the cylindrical portion 24 ′ is substantially the same as the thickness of the flange 23.
  • a central thick portion 25 is connected to the lower end of the cylindrical portion 24 ′.
  • FIG. 11 shows the assembled state of the cell storage container A14.
  • the lid 2 is configured to include the first lid member 2A and the second lid member 2B.
  • the second lid member 2B is formed of a flexible material and is held by the first lid member 2A.
  • the second lid member 2B can be made thicker A certain flange 23 is pinched by the peripheral edge of the opening 110 (upper end 114 of the first cylindrical portion 11) in the container body 1 and the top plate 21 of the first lid member 2A, and the assembly state is maintained.
  • the second lid member 2B is in the form of a series of films ranging from the flange 23 on the outer peripheral side to the thin portion 26 at the center. As a result, the contents (cultured cells and culture medium) contained in the container body 1 are liquid-sealed.
  • the contents of the container body 1 can be appropriately liquid-sealed by the cooperation of the container body 1, the first lid member 2A and the second lid member 2B. it can.
  • the thin portion 26 of the second lid member 2B closes the inside of the flange 23.
  • the thin portion 26 overlaps at least a part of the through hole 211 surrounded by the top plate portion 21 in the thickness direction of the top plate portion 21 of the first lid member 2A.
  • the container body 1 can be filled with the culture medium without leaving a space in the container body 1, it is possible to eliminate the waving of the contents (such as the culture medium) due to the vibration during the culture state maintenance transport. As a result, the cells and living tissues immersed in the culture medium in the container body 1 can be protected from damage and safely maintained in culture and transported.
  • the state near weightlessness is created by rotating a cell storage container 360 degrees vertically and horizontally.
  • the experimental results may be adversely affected, but the cell storage container A14 of this modification can be advantageously used in such applications.
  • the thin portion 26 of the second lid member 2B has gas permeability. Therefore, the contents of the container body 1 are maintained in the ventilated state with the outside of the container body 1. Therefore, according to the present modification, it is possible to culture the contents of the container body 1 in a ventilated state at the time of sealed storage or transport.
  • the thin portion 26 is transparent. According to such a configuration, it is possible to observe the inside of the container body 1 from the upper part of the cell storage container A14 through the transparent thin portion 26.
  • the lid 2 (second lid member 2B) has a central thick portion 25 (protrusion), and the central thick portion 25 is directed to the lower side (the other side in the first direction) inside the container body 1. It is sticking out. According to such a configuration, the storage space for the culture medium and the like in the container body 1 is reduced. Therefore, it is possible to reduce the amount of medium used in maintaining and transporting the culture state.
  • the lid 2 After transporting the cell storage container A14, the lid 2 can be removed from the container 1 by loosening the screw locking portions of the container 1 and the lid 2, and the upper portion of the container 1 can be opened. Therefore, unlike the case of closing the container opening with an adhesive film or the like, when the lid 2 is removed, the problem that the contents of the container body 1 overflow is prevented.
  • the configuration of the lid 2 is different from that of the above-mentioned cell container A14 shown in FIG.
  • the lid 2 has two ports 281 and 282.
  • the ports 281 and 282 are integrally formed at appropriate positions of the top plate portion 21 (first lid member 2A). Also, the ports 281 and 282 are inserted into the port through holes 231 formed in the flange 23 (second lid member 2B). The inner diameter of the port through hole 231 is slightly smaller than the outer diameter of the ports 281 and 282 in the natural state. The ports 281 and 282 are press-fit into the port through holes 231. Thus, the port through hole 231 in which the ports 281 and 282 are inserted is sealed.
  • the ports 281 and 282 are for supplying a culture medium or the like to the inside of the container body 1 or discharging the culture medium in the container body 1 to the outside.
  • the ports 281 and 282 each open at the upper end to the outside and open at the lower end of the inner space of the container 1.
  • a tube not shown is connected as appropriate.
  • one port 281 is used to supply, for example, a medium or the like to the inside of the container body 1.
  • the other port 282 is used to discharge the culture medium and the like inside the container body 1.
  • FIG. 13 shows the assembled state of the cell storage container A15 when the inside of the container body 1 is filled with the medium M1.
  • the lid 2 is configured to include the first lid member 2A and the second lid member 2B.
  • the second lid member 2B is formed of a flexible material and is held by the first lid member 2A.
  • the second lid member 2B can be made thicker A certain flange 23 is pinched by the peripheral edge of the opening 110 (upper end 114 of the first cylindrical portion 11) in the container body 1 and the top plate 21 of the first lid member 2A, and the assembly state is maintained.
  • the second lid member 2B is in the form of a series of films ranging from the flange 23 on the outer peripheral side to the thin portion 26 at the center. As a result, the contents (cultured cells and culture medium) contained in the container body 1 are liquid-sealed.
  • the contents of the container body 1 can be appropriately liquid-sealed by the cooperation of the container body 1, the first lid member 2A and the second lid member 2B. it can.
  • a medium, a cell suspension and a predetermined gas for example, CO 2 gas etc.
  • a predetermined gas for example, CO 2 gas etc.
  • a tube (not shown) connected to the port 281 is connected to a container filled with, for example, a culture medium, a cell suspension, or a predetermined atmosphere gas, and a desired supply can be obtained from these containers by switching valves or the like. It is appropriately selected and supplied into the container 1 through the port 281.
  • the temperature adjustment function of the automatic culture apparatus maintains the inside of the container body 1 at a predetermined temperature.
  • the culture medium in the container body 1 is appropriately discharged and replaced via the port 282.
  • the thin portion 26 of the second lid member 2B closes the inside of the flange 23.
  • the thin portion 26 overlaps at least a part of the through hole 211 surrounded by the top plate portion 21 in the thickness direction of the top plate portion 21 of the first lid member 2A.
  • the container body 1 can be filled with the culture medium without leaving a space in the container body 1, it is possible to eliminate the waving of the contents (such as the culture medium) due to the vibration during the culture state maintenance transport. As a result, the cells and living tissues immersed in the culture medium in the container body 1 can be protected from damage and safely maintained in culture and transported.
  • the state near weightlessness is created by rotating a cell storage container 360 degrees vertically and horizontally.
  • the experimental results may be adversely affected, but the cell storage container A15 of this modification can be advantageously used in such applications.
  • the thin portion 26 of the second lid member 2B has gas permeability. Therefore, the contents of the container body 1 are maintained in the ventilated state with the outside of the container body 1. Therefore, according to the present modification, it is possible to culture the contents of the container body 1 in a ventilated state at the time of sealed storage or transport.
  • the thin portion 26 is transparent. According to such a configuration, it is possible to observe the inside of the container body 1 from the upper part of the cell storage container A15 via the transparent thin portion 26.
  • the ports 281 and 282 provided in the lid 2 are provided at positions deviated away from the central portion (the central thick portion 25 and the thin portion 26) of the lid 2. According to such a configuration, when the situation in the container body 1 is observed from the upper portion of the cell storage container A15 via the transparent thin portion 26, it is avoided that the ports 281 and 282 are obstructed.
  • the lid 2 (second lid member 2B) has a central thick portion 25 (protrusion), and the central thick portion 25 is directed to the lower side (the other side in the first direction) inside the container body 1. It is sticking out. According to such a configuration, the storage space for the culture medium and the like in the container body 1 is reduced. Therefore, it is possible to reduce the amount of medium used in maintaining and transporting the culture state.
  • the lid 2 After transporting the cell storage container A15, the lid 2 can be removed from the container 1 by loosening the screw locking portions of the container 1 and the lid 2, and the upper portion of the container 1 can be opened. Therefore, unlike the case of closing the container opening with an adhesive film or the like, when the lid 2 is removed, the problem that the contents of the container body 1 overflow is prevented.
  • the cell storage container A16 shown in FIG. 14 further includes a cap 283 that can close the tip of the ports 281 and 282 provided in the lid 2.
  • An external thread is formed on the outer peripheral surface of the tip of the ports 281 and 282.
  • the cap 283 is, for example, configured such that the upper end of a substantially cylindrical tubular portion 284 is closed by a top 285.
  • a female screw is formed on the inner peripheral surface of the cylindrical portion 284, and a rubber sheet-like seal member 286 is provided on the top 285, for example. Then, by tightening the above-mentioned female screw of the cap 283 to the above-mentioned male screw of each port 281, 282, the tip of the port 281, 282 is closed.
  • the ports 281 and 282 are inserted into the port through holes 231 formed in the flange 23 (second lid member 2B), and are supported by the second lid member 2B. Further, around the formation region of the port through hole 231 in the flange 23, a thick portion 232 projecting upward is formed so as to be thicker than the other portions.
  • the inner diameter of the port through hole 231 is slightly smaller than the outer diameter of the ports 281 and 282 in the natural state.
  • the ports 281 and 282 are press-fit into the port through holes 231. Thus, the port through hole 231 in which the ports 281 and 282 are inserted is sealed.
  • the configurations of the first cover member 2A, the second cover member 2B, and the soft container member 3 are different from those of the above-described cell storage container A10.
  • the fourth protrusion 27 is provided on the second lid member 2B, but the fourth protrusion 27 is not provided in the cell storage container A10.
  • the bottom wall 31 of the soft container member 3 is provided with a plurality of first protrusions 35.
  • the first protrusions 35 protrude upward (one side in the first direction) from the bottom wall 31.
  • the first protrusions 35 are radially provided in the vertical direction (first direction).
  • the bottom wall 31 has a uniform thickness, and does not have the container member thin portion 311 in the cell storage container A10.
  • the outer peripheral end of each first projection 35 is connected to the cylindrical side wall 321, and the inner peripheral end is positioned near the center of the bottom wall 31.
  • the lower surface of the central thick portion 25 and the lower surface of the thin portion 26 are flush with each other.
  • the gap between the tip of the first projection 35 and the lower surface of the central thick portion 25 or the lower surface of the thin portion 26 is relatively narrow.
  • a convex portion 212 is provided on the top plate portion 21 of the first lid member 2A. As well illustrated in FIG. 18, the convex portion 212 protrudes upward (one side in the first direction) from the main surface 21 a facing the thickness direction of the top plate portion 21.
  • a plurality of convex portions 212 are provided separately from one another.
  • a plane P1 formed by the tips 212a of the convex portions 212 is substantially parallel to the major surface 21a of the top plate 21.
  • substantially parallel is designed such that the plane P1 and the main surface 21a are parallel to each other, but includes cases where there is a slight variation in parallelism due to dimensional tolerance or the like. Further, as understood from FIGS.
  • the convex portion 212 is provided in a circular arc shape of a concentric circle when viewed in the vertical direction, and a gap is formed between adjacent convex portions 212. There is. Thus, the convex portion 212 does not form a closed ring in the vertical direction (in the thickness direction of the top plate portion 21).
  • the bottom portion 12 of the container body 1 is connected to a skirt portion 121 projecting downward from the outer peripheral edge thereof.
  • the outer diameter dimension of the outer peripheral edge of the concentric circular arc formed by the plurality of convex portions 212 is slightly smaller than the inner diameter dimension of the skirt portion 121. According to such a configuration, it is possible to overlap the other cell storage container A18 such that the convex portion 212 of the cell storage container A18 fits into the skirt portion 121 of the other cell storage container A18. Therefore, the plurality of cell storage containers A18 can be stacked in a stable posture.
  • FIG. 19 shows an assembled state of the cell storage container A18 when the inside of the soft container member 3 is filled with the culture medium M1.
  • the lid 2 is configured to include the first lid member 2A and the second lid member 2B.
  • the second lid member 2B is formed of a flexible material and is held by the first lid member 2A.
  • the second lid member 2B can be made thicker A certain flange 23 is pinched by the peripheral edge of the opening 110 (upper end 114 of the first cylindrical portion 11) in the container body 1 and the top plate 21 of the first lid member 2A, and the assembly state is maintained.
  • the second lid member 2B is in the form of a series of films ranging from the flange 23 on the outer peripheral side to the thin portion 26 at the center.
  • the thin-walled portion 26 of the second lid member 2B closes the inside of the flange 23.
  • the thin portion 26 overlaps at least a part of the through hole 211 surrounded by the top plate portion 21 in the thickness direction of the top plate portion 21 of the first lid member 2A.
  • the internal volume is displaced so as to slightly increase (displacement upward in FIG. 19), and it is possible to suppress air bubbles from being trapped in the flexible container member 3. Therefore, since the whole inside of the soft container member 3 can be filled with the culture medium without leaving a space in the soft container member 3, it is possible to eliminate the waving of contents (such as the culture medium) due to vibration etc. it can. As a result, the cells and living tissues immersed in the medium in the soft container member 3 can be protected from damage and safely maintained in culture and transported.
  • the state near weightlessness is created by rotating a cell storage container 360 degrees vertically and horizontally.
  • the experimental results may be adversely affected, but the cell storage container A18 of this modification can be advantageously used in such applications.
  • the thin-walled portion 26 of the second lid member 2B has gas permeability. For this reason, with respect to the contents of the container body 1 (soft container member 3), the ventilation state with the outside of the container body 1 (soft container member 3) is maintained. Therefore, according to this modification, it is possible to culture the contents of the container body 1 (soft container member 3) in a ventilated state during sealed storage or transportation.
  • the thin portion 26 is transparent. According to such a configuration, it is possible to observe the condition in the container body 1 (soft container member 3) from the top of the cell storage container A18 via the transparent thin portion 26.
  • a soft container member 3 is interposed between the container body 1 and the lid 2 (second lid member 2B), and the culture container or the living tissue and the culture medium are accommodated in the soft container member 3. Ru. And since this soft container member 3 has flexibility, even if the cell and biological tissue accommodated in the inside of the soft container member 3 collide directly with the said soft container member 3, it does not damage easily. Therefore, the cell storage container A18 having the soft container member 3 is suitable for culture state maintenance transport.
  • the bottom wall 31 of the flexible container member 3 is provided with a plurality of first protrusions 35, and these first protrusions 35 protrude upward (one side in the first direction).
  • the support 4 is formed between the central thick portion 25 or the thin portion 26 on the second lid member 2B side and the first projection 35 on the soft container member 3 side.
  • the support 4 has, for example, a frame 41 and a thin film 42.
  • the frame 41 has a ring shape, and the thin film 42 is formed to close the inside of the frame 41.
  • the thin film 42 is a part on which the cultured cells (cell sheet) are placed, and the cultured cells on the thin film 42 are immersed in the medium. Therefore, according to this modification, culture of a cell sheet is appropriately performed via the stably arranged support 4.
  • the soft container member 3 when changing the inner volume of the sealed container according to, for example, the cultured cells and the living tissue contained in the sealed container, or the size of the support 4, etc. It is easily coped with by changing the size of 3.
  • the lid 2 (second lid member 2B) has a central thick portion 25 (protrusion), and the central thick portion 25 is a lower portion (first direction) inside the container 1 (soft container member 3). It projects towards the other side). According to such a configuration, the storage space for the culture medium and the like in the soft container member 3 is reduced. Therefore, the amount of use of the culture medium M1 can be reduced at the time of culture state maintenance transport.
  • the lid 2 After transporting the cell storage container A18, the lid 2 can be removed from the container 1 by loosening the screw locking portions of the container 1 and the lid 2, and the upper portion of the container 1 (soft container member 3) It can be opened. Therefore, unlike the case where the container opening is closed with an adhesive film or the like, when the lid 2 is removed, the problem that the contents of the container body 1 (soft container member 3) overflows is prevented.
  • a convex portion 212 is provided on the top plate portion 21 of the first lid member 2A. According to such a configuration, it is convenient when the cell storage container A18 is stored in another storage container and transported. At the time of transportation of the cell storage container A18, for example, the container body 1 and the lid 2 are sandwiched from above and below by the container member and the lid member of the storage container.
  • the plane P1 formed by the tip end 212a of the convex portion 212 is substantially parallel to the main surface 21a of the top plate portion 21.
  • the convex portion 212 does not form a closed annular shape in the vertical direction view (view in the thickness direction of the top plate portion 21).
  • the container body 1 (soft container member 3) can be maintained and transported in a culture state under a predetermined gas environment while maintaining the ventilation state with the outside (the internal space of the storage container).
  • the configuration of the soft container member 3 is different from that of the above-described cell storage container A18. Specifically, the configuration of the plurality of first protrusions 35 provided on the bottom wall 31 is different. Also, instead of the annular protrusion 34 in the cell storage container A18, a plurality of extended pieces 36 are provided.
  • the plurality of first protrusions 35 are formed by raising a predetermined portion portion of the bottom wall 31 upward (one side in the first direction). It is.
  • Each of the first protrusions 35 is formed substantially in a fan shape, and is radially provided so as to be equally spaced in a vertical direction view (first direction view).
  • the whole may be partially connected upward from the flat bottom wall 31 like the above-mentioned cell storage container A18 etc.
  • a part of the bottom wall 31 itself may be configured to bulge upward.
  • Each of the plurality of extending pieces 36 extends radially outward from the outer peripheral surface of the inclined side wall portion 322.
  • the plurality of extending pieces 36 are provided at equal intervals in the circumferential direction of the inclined side wall portion 322.
  • the plurality of extension pieces 36 contact the inner peripheral surface of the first cylindrical portion 11 of the container body 1 when setting the soft container member 3 inside the container body 1.
  • the plurality of extension pieces 36 function as positioning at the time of setting the soft container member 3 inside the container body 1.
  • a plurality of convex portions 212 are provided on the top plate portion 21 of the first lid member 2A. Further, a skirt portion 121 is provided at the bottom portion 12 of the container body 1.
  • the configuration of the convex portion 212 and the skirt portion 121 is the same as that of the above-described cell storage container A18. According to the configuration including the convex portion 212 and the skirt portion 121, the convex portion 212 of the cell storage container A19 is fitted to the skirt portion 121 of the other cell storage container A19 so as to fit the other cell storage container A19. Can be superimposed. Therefore, the plurality of cell storage containers A19 can be stacked in a stable posture.
  • FIG. 24 shows the assembled state of the cell storage container A19 when the inside of the soft container member 3 is filled with the culture medium M1.
  • the lid 2 is configured to include the first lid member 2A and the second lid member 2B.
  • the second lid member 2B is formed of a flexible material and is held by the first lid member 2A.
  • the second lid member 2B can be made thicker A certain flange 23 is pinched by the peripheral edge of the opening 110 (upper end 114 of the first cylindrical portion 11) in the container body 1 and the top plate 21 of the first lid member 2A, and the assembly state is maintained.
  • the second lid member 2B is in the form of a series of films ranging from the flange 23 on the outer peripheral side to the thin portion 26 at the center.
  • the thin-walled portion 26 of the second lid member 2B blocks the inside of the flange 23.
  • the thin portion 26 overlaps at least a part of the through hole 211 surrounded by the top plate portion 21 in the thickness direction of the top plate portion 21 of the first lid member 2A.
  • the internal volume is displaced so as to slightly increase (displacement upward in FIG. 24), and it is possible to suppress air bubbles from being trapped in the flexible container member 3. Therefore, since the whole inside of the soft container member 3 can be filled with the culture medium without leaving a space in the soft container member 3, it is possible to eliminate the waving of contents (such as the culture medium) due to vibration etc. it can. As a result, the cells and living tissues immersed in the medium in the soft container member 3 can be protected from damage and safely maintained in culture and transported.
  • the state near weightlessness is created by rotating a cell storage container 360 degrees vertically and horizontally.
  • the experimental results may be adversely affected.
  • the cell storage container A19 of this modification can be advantageously used in such applications.
  • the thin-walled portion 26 of the second lid member 2B has gas permeability. For this reason, with respect to the contents of the container body 1 (soft container member 3), the ventilation state with the outside of the container body 1 (soft container member 3) is maintained. Therefore, according to this modification, it is possible to culture the contents of the container body 1 (soft container member 3) in a ventilated state during sealed storage or transportation.
  • the thin portion 26 is transparent. According to such a configuration, it is possible to observe the inside of the container body 1 (soft container member 3) from the upper part of the cell storage container A19 through the transparent thin portion 26.
  • a soft container member 3 is interposed between the container body 1 and the lid 2 (second lid member 2B), and the culture container or the living tissue and the culture medium are accommodated in the soft container member 3. Ru. And since this soft container member 3 has flexibility, even if the cell and biological tissue accommodated in the inside of the soft container member 3 collide directly with the said soft container member 3, it does not damage easily. Therefore, the cell storage container A19 having the soft container member 3 is suitable for culture state maintenance transport.
  • the bottom wall 31 of the flexible container member 3 is provided with a plurality of first protrusions 35, and these first protrusions 35 protrude upward (one side in the first direction).
  • the support 4 is provided between the central thick portion 25 or the thin portion 26 on the second lid member 2B side and the first projection 35 on the soft container member 3 side.
  • the support 4 has, for example, a frame 41 and a thin film 42.
  • the frame 41 has a ring shape, and the thin film 42 is formed to close the inside of the frame 41.
  • the thin film 42 is a part on which the cultured cells (cell sheet) are placed, and the cultured cells on the thin film 42 are immersed in the medium. Therefore, according to this modification, culture of a cell sheet is appropriately performed via the stably arranged support 4.
  • the soft container member 3 when changing the inner volume of the sealed container according to, for example, the cultured cells and the living tissue contained in the sealed container, or the size of the support 4, etc. It is easily coped with by changing the size of 3.
  • the lid 2 (second lid member 2B) has a central thick portion 25 (protrusion), and the central thick portion 25 is a lower portion (first direction) inside the container 1 (soft container member 3). It projects towards the other side). According to such a configuration, the storage space for the culture medium and the like in the soft container member 3 is reduced. Therefore, the amount of use of the culture medium M1 can be reduced at the time of culture state maintenance transport.
  • the first protrusion 35 provided on the soft container member 3 is configured such that a part of the bottom wall 31 bulges upward. According to such a configuration, the storage space for the culture medium and the like in the soft container member 3 is further reduced. Therefore, the amount of culture medium used can be further reduced during culture state maintenance transport.
  • the lid 2 After transporting the cell storage container A19, the lid 2 can be removed from the container 1 by loosening the screw locking portions of the container 1 and the lid 2, and the upper portion of the container 1 (soft container member 3) It can be opened. Therefore, unlike the case where the container opening is closed with an adhesive film or the like, when the lid 2 is removed, the problem that the contents of the container body 1 (soft container member 3) overflows is prevented.
  • a convex portion 212 is provided on the top plate portion 21 of the first lid member 2A. According to such a configuration, it is convenient when the cell storage container A19 is stored and transported in another storage container. At the time of transportation of the cell storage container A19, the container body 1 and the lid 2 are sandwiched from above and below, for example, by the container member and the lid member of the storage container.
  • the plane P1 formed by the tip end 212a of the convex portion 212 is substantially parallel to the main surface 21a of the top plate portion 21.
  • the convex portion 212 does not form a closed annular shape in the vertical direction view (view in the thickness direction of the top plate portion 21).
  • the container body 1 (soft container member 3) can be maintained and transported in a culture state under a predetermined gas environment while maintaining the ventilation state with the outside (the internal space of the storage container).
  • the cell storage container A20 of the present embodiment includes the container body 1 and the lid 2 and is configured as a so-called culture flask.
  • the container body 1 is a culture flask provided with the first cylindrical portion 11 and the container portion 14.
  • the container portion 14 has a bottom wall portion 141, a front wall portion 142, a rear wall portion 143, a pair of side wall portions 144, and a ceiling wall portion 145, each rising from the periphery of the bottom wall portion 141.
  • the container portion 14 has a predetermined internal space surrounded by these elements.
  • the first tubular portion 11 is connected to the center of the front wall portion 142.
  • the first tubular portion 11 extends along the direction in which the axis Ox extending at a predetermined angle extends.
  • the first cylindrical portion 11 is a portion that carries in and out cells and culture medium with respect to the container portion 14.
  • the first tubular portion 11 is cylindrical, and has an opening 110 at its tip (one side end in the first direction). Further, the first cylindrical portion 11 is inclined so as to be displaced upward as it goes to the tip (as it gets farther from the front wall portion 142).
  • An external thread 111 is formed on the outer peripheral surface of the first tubular portion 11.
  • the container body 1 is formed of, for example, a translucent or transparent plastic material.
  • the plastic material for example, besides polystyrene and methylpentene, materials having transparency such as polycarbonate, cycloolefin polymer, cycloolefin copolymer and the like are preferably used, but the material is not limited thereto.
  • the lid 2 includes a first lid 2A and a second lid 2B, and is for closing the opening 110 of the container 1 and sealing the container 1.
  • the first lid member 2A has a top plate portion 21 and a second cylindrical portion 22.
  • the top plate portion 21 has a substantially annular shape, and between the flange 23 of the second lid member 2B described later and the tip end portion 115 of the first cylindrical portion 11 of the container body 1 (peripheral portion of tip end opening) It is a part to pinch.
  • a through hole 211 is formed on the inner side in the radial direction of the top plate portion 21. The through hole 211 is surrounded by the top plate 21 in the thickness direction of the top plate 21.
  • the second cylindrical portion 22 extends from the peripheral edge of the top plate 21 in the thickness direction (downward in the drawing) of the top plate 21 and is substantially cylindrical.
  • a female screw 221 is formed on the inner peripheral surface of the second cylindrical portion 22, and the female screw 221 can be screwed to the male screw 111 of the container body 1 (first cylindrical portion 11).
  • the first lid member 2A may be formed of the same material as that of the container body 1 or may be formed of a plastic material that is more excellent in slidability.
  • a plastic material which is excellent in slidability polyethylene and polypropylene are mentioned, for example.
  • the second lid member 2B is, for example, a rubber molded product, and has a flange 23, a cylindrical portion 24 ', a central thick portion 25 (projecting portion), and a thin portion 26.
  • the flange 23 has a substantially annular shape, and overlaps with the top plate 21 in the thickness direction of the top plate 21.
  • the flange 23 has an appropriate thickness and has an appropriate elastic restoring force with respect to the load in the direction (first direction) in which the axis Ox extends.
  • the thickness of the flange 23 is, for example, about 1 to 3 mm.
  • the flange 23 is interposed between the peripheral edge (the tip 115 of the first cylindrical portion 11) of the opening 110 in the container body 1 and the top plate 21 in the first lid member 2A. ing.
  • the cylindrical portion 24 ' is connected to the inner peripheral edge of the flange 23 and extends in the direction in which the axis Ox extends, to the side of the container portion 14 (the other side in the first direction).
  • the thickness of the cylindrical portion 24 ′ is substantially the same as the thickness of the flange 23.
  • the central thick portion 25 is connected to the tip end edge of the cylindrical portion 24 ′ and extends radially inward, and has a substantially annular shape.
  • the central thick portion 25 is accommodated inside the first cylindrical portion 11 of the container 1 at a distance from the first cylindrical portion 11 and protrudes toward the container portion 14 (the other side in the first direction).
  • the central thick portion 25 corresponds to the projecting portion in the present invention.
  • the thickness of the central thick portion 25 is approximately the same as the thickness of the flange 23.
  • the thin portion 26 is provided at the center of the second lid member 2B.
  • the thin portion 26 closes the radially inner side of the central thick portion 25 (flange 23).
  • the thin portion 26 is a portion whose thickness is reduced compared to the other portions.
  • the thin portion 26 has a substantially circular shape in a direction view (first direction view) in which the axis Ox extends.
  • the thickness of the thin portion 26 is, for example, about 0.2 to 0.3 mm.
  • the thin portion 26 has gas permeability.
  • the thin portion 26 is transparent. The thin portion 26 overlaps the through hole 211 surrounded by the top plate 21 in the thickness direction of the top plate 21.
  • the second lid member 2B is made of a flexible and elastic material.
  • a material which constitutes the 2nd lid member 2B silicone rubber, natural rubber, urethane rubber, elastomer resin, etc. are mentioned, for example.
  • the material of the second lid member 2B is non-cytotoxic and is a biocompatible silicone rubber for medical use. Is more preferred.
  • the hardness of the second lid member 2B it is preferable that, for example, the rubber hardness is approximately 20 degrees to 40 degrees.
  • the outer diameter of the flange 23 of the second lid 2B is slightly larger than the inner diameter of the second cylindrical portion 22 of the first lid 2A in the natural state.
  • the second lid member 2B is held by the first lid member 2A by being fitted inside the second cylindrical portion 22 while the flange 23 is elastically compressed.
  • the cell storage container A20 stores the cultured cells, the living tissue and the culture medium in the container body 1 (in the present embodiment, particularly the container portion 14), hermetically stores it while maintaining the culture state, and transports it (culture state maintenance transport) Used for The cultured cells, the living tissue, and the culture medium accommodated in the container body 1 (container unit 14) are not particularly limited.
  • cells cultured in an adherent state on a culture surface (upper surface of the bottom wall 141) of a flask used for research, or cell preparations used for regenerative medicine can be stored in a suspension.
  • the whole inside of container body 1 (container part 14) is filled with the medium to prevent movement of the medium by waving the medium, and the movement of the medium is prevented. Need to reduce
  • the lid 2 is configured to include the first lid member 2A and the second lid member 2B.
  • the second lid member 2B is formed of a flexible material and is held by the first lid member 2A. Then, by tightening the female screw 221 of the first lid member 2A (second tubular portion 22) into the male screw 111 of the container body 1 (first tubular portion 11), the second lid member 2B can be made thicker A certain flange 23 is sandwiched between the peripheral edge of the opening 110 in the container body 1 (the tip 115 of the first tubular portion 11) and the top plate 21 of the first lid member 2A, and the assembly state is maintained.
  • the second lid member 2B is in the form of a series of films ranging from the flange 23 on the outer peripheral side to the thin portion 26 at the center.
  • the contents (cultured cells and culture medium) contained in the container body 1 are liquid-sealed. Therefore, the contents do not overflow due to vibration or the like during culture state maintenance transport.
  • the contents of the container body 1 (container portion 14) can be appropriately obtained by the cooperation of the container body 1, the first lid member 2A and the second lid member 2B. It can be liquid sealed.
  • the thin portion 26 of the second lid member 2B closes the inside of the flange 23.
  • the thin portion 26 overlaps at least a part of the through hole 211 surrounded by the top plate portion 21 in the thickness direction of the top plate portion 21 of the first lid member 2A. According to such a configuration, when the container body 1 (container portion 14) is sealed, the flexible thin portion 26 can be displaced following the change in the internal pressure of the container.
  • the thin portion 26 absorbs the internal pressure change It can be displaced (displaced outward in the direction along the axis Ox in FIG. 26) so that the internal volume slightly increases, and air bubbles can be prevented from being trapped in the container body 1. Therefore, since the entire inside of the container body 1 can be filled with the culture medium without leaving a space in the container body 1, it is possible to eliminate the waving of the contents (such as the culture medium) due to the vibration during the culture state maintenance transport. As a result, the cells and living tissues immersed in the culture medium in the container body 1 can be protected from damage and safely maintained in culture and transported.
  • the state near weightlessness is created by rotating a cell storage container 360 degrees vertically and horizontally.
  • the experimental results may be adversely affected.
  • the cell storage container A20 of the present embodiment can be advantageously used in such applications.
  • the thin portion 26 of the second lid member 2B also has gas permeability. Therefore, the contents of the container body 1 (container portion 14) are maintained in the ventilated state with the outside of the container body 1 (container portion 14). Therefore, according to the present embodiment, it is possible to culture the contents of the container body 1 (container portion 14) in a ventilated state during sealed storage or transportation.
  • the lid 2 (second lid member 2B) has a central thick portion 25 (protrusion), and the central thick portion 25 is a direction in which the axis Ox extends in the inside of the container body 1 and the container portion 14 It protrudes toward the side (the other side in the first direction). According to such a configuration, the storage space for the culture medium and the like in the container body 1 is reduced. Therefore, it is possible to reduce the amount of medium used in maintaining and transporting the culture state.
  • FIG. 27 and FIG. 28 show a modification of the cell storage container A20 described above.
  • the configuration of the second lid member 2B is different from that of the cell container A20 described above.
  • a part of the flange 23 protrudes radially inward, and serves as a protruding portion 233.
  • the flange 23 (the overhanging portion 233) has an appropriate thickness as described above and also has an elastic restoring force.
  • the flange 23 (the overhanging portion 233) has resealability for closing the piercing hole of the injection needle.
  • the contents for example, the cell suspension soaked in the culture medium
  • a syringe (not shown).
  • the contents can be taken out by sticking an injection needle (not shown) into the extension part 233 and sucking it up.
  • the contents can be taken out while maintaining the sealed state without removing the lid 2 from the container body 1.
  • the cell storage container A30 of the present embodiment includes the container body 1 and the lid 2 and is configured as a container having a so-called hinge-type lid.
  • the container body 1 is provided with the first cylindrical portion 11 and the container portion 15 and is in the shape of a container having the opening 110 at the upper end (first direction one side end) of the first cylindrical portion 11 There is.
  • the container portion 15 has a bottom wall 151 and a cylindrical portion 152 rising from the periphery of the bottom wall 151.
  • the first cylindrical portion 11 is connected to the upper end of the cylindrical portion 152.
  • the diameter dimension of the first cylindrical portion 11 is larger than the diameter dimension of the cylindrical portion 152, and the first cylindrical portion 11 and the cylindrical portion 152 (the container portion 15) are connected via the step portion 153. There is.
  • the first cylindrical portion 11 is a portion that carries in and out cells and culture medium with respect to the container portion 15.
  • the first cylindrical portion 11 has a substantially cylindrical shape, and has an opening 110 at the upper end (one side end in the first direction).
  • the container body 1 is formed of, for example, a translucent or transparent plastic material.
  • the plastic material for example, besides polystyrene and methylpentene, materials having transparency such as polycarbonate, cycloolefin polymer, cycloolefin copolymer and the like are preferably used, but the material is not limited thereto.
  • the lid 2 includes a first lid 2A and a second lid 2B, and is for closing the opening 110 of the container 1 and sealing the container 1.
  • the first lid member 2A has a top plate portion 21, a second cylindrical portion 22, an annular mounting portion 291, a connecting portion 292, and a locking portion 293.
  • the top plate portion 21 has a substantially annular shape, and between the flange 23 of the second lid member 2B described later and the upper end portion 114 (the peripheral edge portion of the upper end opening) of the first cylindrical portion 11 of the container 1 It is a part to pinch.
  • the top plate portion 21 has a cylindrical extending portion 213 extending downward (the other side in the first direction) from the inner peripheral edge thereof.
  • a through hole 211 is formed on the inner side in the radial direction of the top plate portion 21 (cylindrical extension portion 213). The through hole 211 is surrounded by the top plate 21 in the thickness direction of the top plate 21.
  • the second cylindrical portion 22 extends from the peripheral edge of the top plate 21 in the thickness direction (downward in the drawing) of the top plate 21 and is substantially cylindrical.
  • the annular mounting portion 291 is a portion that is externally fitted to the container body 1. Specifically, the annular mounting portion 291 is externally fitted to the cylindrical portion 152 of the container portion 15 and is locked in a state adjacent to the lower side of the step portion 153.
  • connection portion 292 connects the annular mounting portion 291 and the top plate portion 21.
  • One end of the connecting portion 292 is connected to the annular mounting portion 291, and the other end of the connecting portion 292 is connected to the top plate portion 21.
  • the connecting portion 292 is formed with a bendable portion 292a along a direction perpendicular to the longitudinal direction from one end to the other end.
  • the easily bendable portion 292a is easily bent due to its small thickness compared to the other portions.
  • the bendable portions 292a are provided at two places.
  • the connection portion 292 is bent in a state in which the opening 110 of the container 1 is closed by the lid 2 (see FIG. 30), and the connection portion 292 extends straight in a state in which the opening 110 is open (see FIG. 32).
  • the locking portion 293 is for maintaining the state in which the opening 110 of the container 1 is closed by the lid 2 and has a locking protrusion 293 a. As shown in FIG. 30, in the state where the connecting portion 292 is bent and the opening 110 of the container 1 is closed by the lid 2, the locking projection 293a is formed in the locking recess 291a provided in the annular mounting portion 291. When engaged, the lid 2 maintains the state of closing the opening 110 of the container 1.
  • the first lid member 2A is formed of, for example, a plastic material capable of forming a hinge portion which can be easily bent.
  • plastic material include, but not limited to, polypropylene.
  • the second lid member 2B is, for example, a rubber molded product, and has a flange 23, a cylindrical portion 24 ', a central thick portion 25 (projecting portion), and a thin portion 26.
  • the flange 23 has a substantially annular shape, and overlaps with the top plate 21 in the thickness direction of the top plate 21.
  • the flange 23 has an appropriate thickness and has an appropriate elastic restoring force with respect to a load in the vertical direction (first direction).
  • the thickness of the flange 23 is, for example, about 1 to 3 mm.
  • the flange 23 is the peripheral portion (the upper end 114 of the first cylindrical portion 11) of the opening 110 in the container 1 and the first lid member 2A. It is interposed between the top plate portion 21.
  • the cylindrical portion 24 ' is connected to the inner peripheral edge of the flange 23 and extends downward (the other side in the first direction).
  • the thickness of the cylindrical portion 24 ′ is substantially the same as the thickness of the flange 23.
  • the central thick portion 25 is connected to the tip end edge of the cylindrical portion 24 ′ and extends radially inward, and has a substantially annular shape.
  • the central thick portion 25 is accommodated inside the first cylindrical portion 11 of the container 1 at a distance from the first cylindrical portion 11 and protrudes downward (the other side in the first direction).
  • the central thick portion 25 corresponds to the projecting portion in the present invention.
  • the thickness of the central thick portion 25 is approximately the same as the thickness of the flange 23.
  • the thin portion 26 is provided at the center of the second lid member 2B.
  • the thin portion 26 closes the radially inner side of the central thick portion 25 (flange 23).
  • the thin portion 26 is a portion whose thickness is reduced compared to other portions.
  • the thin portion 26 has a substantially circular shape in the vertical direction (first direction).
  • the thickness of the thin portion 26 is, for example, about 0.2 to 0.3 mm.
  • the thin portion 26 has gas permeability.
  • the thin portion 26 is transparent. The thin portion 26 overlaps the through hole 211 surrounded by the top plate 21 in the thickness direction of the top plate 21.
  • the second lid member 2B is made of a flexible and elastic material.
  • a material which constitutes the 2nd lid member 2B silicone rubber, natural rubber, urethane rubber, elastomer resin, etc. are mentioned, for example.
  • the material of the second lid member 2B is non-cytotoxic and is a biocompatible silicone rubber for medical use. Is more preferred.
  • the hardness of the second lid member 2B it is preferable that, for example, the rubber hardness is approximately 20 degrees to 40 degrees.
  • the outer diameter of the flange 23 of the second lid 2B is slightly larger than the inner diameter of the second cylindrical portion 22 of the first lid 2A in the natural state.
  • the second lid member 2B is held by the first lid member 2A by being fitted inside the second cylindrical portion 22 while the flange 23 is elastically compressed.
  • the cell storage container A30 is used for storing the cultured cells or the biological tissue or the medium in the container body 1 and keeping it in a sealed state while keeping the state of culture and transporting (culture state maintenance transport).
  • the cultured cells, the living tissue, and the culture medium accommodated in the container body 1 (container portion 15) are not particularly limited.
  • cell preparations for use in regenerative medicine can be in the form of a suspension or in the form of cell sheets.
  • container body 1 container part 15
  • the entire inside of container body 1 is filled with the medium to prevent movement of the medium in order to prevent the cells from being damaged by the medium being shaken. I need to suppress it.
  • the lid 2 is configured to include the first lid member 2A and the second lid member 2B.
  • the second lid member 2B is formed of a flexible material and is held by the first lid member 2A.
  • the opening 110 of the container 1 is closed by the lid 2, and the locking projection 293 a (locking portion 293) engages with the locked recess 291 a of the annular mounting portion 291, thereby the second lid member 2 B.
  • the thick flange 23 is held between the peripheral portion (the tip 115 of the first cylindrical portion 11) of the opening 110 of the container 1 and the top plate 21 of the first lid member 2A. The closed state of the opening 110 is maintained.
  • the second lid member 2B is in the form of a series of films ranging from the flange 23 on the outer peripheral side to the thin portion 26 at the center.
  • the locking projection 293a (locking portion 293) of the first lid member 2A and the locking recess 291a constitute locking means in the present invention.
  • the thin portion 26 of the second lid member 2B closes the inside of the flange 23.
  • the thin portion 26 overlaps at least a part of the through hole 211 surrounded by the top plate portion 21 in the thickness direction of the top plate portion 21 of the first lid member 2A.
  • the container body 1 can be filled with the culture medium without leaving a space in the container body 1, it is possible to eliminate the waving of the contents (such as the culture medium) due to the vibration during the culture state maintenance transport. As a result, the cells and living tissues immersed in the culture medium in the container body 1 can be protected from damage and safely maintained in culture and transported.
  • the state near weightlessness is created by rotating a cell storage container 360 degrees vertically and horizontally.
  • the presence of air bubbles in the container may adversely affect the experimental results, but the cell storage container A30 of the present embodiment can also be advantageously used in such applications.
  • the thin portion 26 of the second lid member 2B also has gas permeability.
  • the contents of the container body 1 (container portion 15) are maintained in the ventilated state with the outside of the container body 1 (container portion 15). Therefore, according to the present embodiment, it is possible to culture the contents of the container body 1 (container portion 15) in a ventilated state during sealed storage or transportation.
  • the thin portion 26 is transparent. According to such a configuration, it is possible to observe the condition in the container body 1 (the container unit 15) from the outside of the cell storage container A30 through the transparent thin portion 26.
  • the lid 2 (second lid member 2B) has a central thick portion 25 (protrusion), and the central thick portion 25 is directed to the lower side (the other side in the first direction) inside the container body 1. It is sticking out. According to such a configuration, the storage space for the culture medium and the like in the container body 1 is reduced. Therefore, it is possible to reduce the amount of medium used in maintaining and transporting the culture state.
  • the lid 2 After transporting the cell storage container A30, the lid 2 can be released from the container 1 by releasing the engagement of the locking portion 293 (locking protrusion 293a) of the container 1 and the lid 2; One opening 110 can be opened. Therefore, unlike the case where the container opening is closed by the adhesive film or the like, when the lid 2 is released, the problem that the contents of the container 1 overflow due to excessive force is prevented.
  • the cell storage container A 40 of the present embodiment includes a container body 1 and a lid 2.
  • the container body 1 is provided with the first cylindrical portion 11 and the container portion 15 and is in the shape of a container having the opening 110 at the upper end (first direction one side end) of the first cylindrical portion 11 There is.
  • the container portion 15 has a bottom wall 151 and a cylindrical portion 152 rising from the periphery of the bottom wall 151.
  • the first cylindrical portion 11 is connected to the upper end of the cylindrical portion 152.
  • the diameter dimension of the first cylindrical portion 11 is larger than the diameter dimension of the cylindrical portion 152, and the first cylindrical portion 11 and the cylindrical portion 152 (the container portion 15) are connected via the step portion 153. There is.
  • the first cylindrical portion 11 is a portion that carries in and out cells and culture medium with respect to the container portion 15.
  • the first cylindrical portion 11 has a substantially cylindrical shape, and has an opening 110 at the upper end (one side end in the first direction).
  • the container body 1 is formed of, for example, a translucent or transparent plastic material.
  • the plastic material for example, besides polystyrene and methylpentene, materials having transparency such as polycarbonate, cycloolefin polymer, cycloolefin copolymer and the like are preferably used, but the material is not limited thereto.
  • the lid 2 is, for example, a rubber molded product, and includes a second cylindrical portion 22, a flange 23, a thick portion 25 ′, a thin portion 26, and a convex portion 234.
  • the lid 2 seals the container 1 by being attached.
  • the flange 23 has an appropriate thickness and has an appropriate elastic restoring force with respect to a load in the vertical direction (first direction).
  • the flange 23 overlaps the peripheral edge portion (the upper end portion 114 of the first cylindrical portion 11) of the opening 110 in the container body 1 as viewed in the thickness direction of the flange 23 ing.
  • the lower surface of the flange 23 can abut on the peripheral edge portion of the opening 110 (the upper end portion 114 of the first cylindrical portion 11).
  • the second cylindrical portion 22 is a portion fitted in and fitted to the first cylindrical portion 11 of the container body 1.
  • the second cylindrical portion 22 extends from the inner peripheral edge of the flange 23 in the thickness direction of the flange 23 and has a substantially cylindrical shape.
  • the outer diameter of the second cylindrical portion 22 in the natural state is larger than the inner diameter of the first cylindrical portion 11.
  • the press-fit of the second cylindrical portion 22 prevents relative movement between the first cylindrical portion 11 and the second cylindrical portion 22.
  • the configuration in which the second cylindrical portion 22 is press-fit into the first cylindrical portion 11 bears the locking means in the present invention.
  • the thickness of the second tubular portion 22 is substantially the same as the thickness of the flange 23.
  • the thick portion 25 ' is connected to the tip end edge of the second cylindrical portion 22 and extends radially inward, and has a substantially annular shape.
  • the thickness of the thick portion 25 ′ is substantially the same as the thickness of the flange 23.
  • the thin portion 26 is provided at the center of the lid 2.
  • the thin-walled portion 26 blocks the radially inner side of the thick-walled portion 25 ′ (flange 23).
  • the thin portion 26 is a portion whose thickness is thinner than other portions.
  • the thin portion 26 has a substantially circular shape in the vertical direction (first direction).
  • the thickness of the thin portion 26 is, for example, about 0.2 to 0.3 mm.
  • the thin portion 26 has gas permeability. Also in the present embodiment, the thin portion 26 is transparent.
  • the convex portion 234 protrudes upward (one side in the first direction) from the major surface 23 a facing the thickness direction of the flange 23.
  • a plurality of convex portions 234 are provided separately from one another.
  • a plane P2 formed by the tips 234a of the convex portions 234 is substantially parallel to the major surface 23a of the flange 23.
  • substantially parallel is designed such that the plane P2 and the main surface 23a are parallel to each other, but includes cases where there is a slight variation in parallelism due to dimensional tolerance or the like. Further, as understood from FIGS.
  • the convex portion 234 is provided in a circular arc shape of a concentric circle in the vertical direction view, and the groove 235 is formed between the adjacent convex portions 234. It is done. Thus, the convex portion 234 does not form a closed ring in the vertical direction (in the thickness direction of the flange 23).
  • the lid 2 described above is made of a material having flexibility and elasticity.
  • a raw material which comprises the cover body 2 silicone rubber, natural rubber, urethane rubber, an elastomer resin etc. are mentioned, for example.
  • the material of the lid 2 is preferably a medical silicone rubber having no cytotoxicity and having biocompatibility.
  • the hardness of the lid 2 is preferably, for example, about 20 degrees to 40 degrees of rubber hardness.
  • the cell storage container A40 is used for storing the cultured cells or the living tissue and the medium in the container body 1, sealing and storing while maintaining the culture state, and transporting (culture state maintenance transport).
  • the cultured cells, the living tissue, and the culture medium accommodated in the container body 1 (container portion 15) are not particularly limited.
  • cell preparations for use in regenerative medicine can be in the form of a suspension or in the form of cell sheets.
  • container body 1 container part 15
  • the entire inside of container body 1 is filled with the medium to prevent movement of the medium in order to prevent the cells from being damaged by the medium being shaken. I need to suppress it.
  • the lid 2 is integrally formed of a flexible material. Then, by inserting the second cylindrical portion 22 of the lid 2 into the first cylindrical portion 11 through the opening 110 of the container body 1, the second cylindrical portion 22 is elastically compressed and the first cylindrical portion 11 is inserted. As a result, the assembly state of the container body 1 and the lid 2 is maintained.
  • the lid 2 is in the form of a series of films ranging from the flange 23 on the outer peripheral side to the central thin portion 26.
  • the contents (cultured cells and culture medium) contained in the container body 1 are liquid-sealed. Therefore, the contents do not overflow due to vibration or the like during culture state maintenance transport.
  • the contents of the container body 1 (container portion 15) can be appropriately liquid-sealed by the cooperation of the container body 1 and the lid 2.
  • the thin-walled portion 26 of the lid 2 closes the inside of the flange 23 and the second cylindrical portion 22.
  • the flexible thin portion 26 can be displaced following the change in the internal pressure of the container.
  • the thin portion 26 absorbs the internal pressure change It can be displaced (displaced upward in the drawing in FIG. 34) so that the internal volume slightly increases, and air bubbles can be prevented from being trapped in the container body 1.
  • the entire inside of the container body 1 can be filled with the culture medium without leaving a space in the container body 1, it is possible to eliminate the waving of the contents (such as the culture medium) due to the vibration during the culture state maintenance transport. As a result, the cells and living tissues immersed in the culture medium in the container body 1 can be protected from damage and safely maintained in culture and transported.
  • the state near weightlessness is created by rotating a cell storage container 360 degrees vertically and horizontally.
  • the experimental results may be adversely affected.
  • the cell storage container A40 of the present embodiment can be advantageously used in such applications.
  • the thin portion 26 of the lid 2 has gas permeability.
  • the contents of the container body 1 (container portion 15) are maintained in the ventilated state with the outside of the container body 1 (container portion 15). Therefore, according to the present embodiment, it is possible to culture the contents of the container body 1 (container portion 15) in a ventilated state during sealed storage or transportation.
  • the thin portion 26 is transparent. According to such a configuration, it is possible to observe the inside of the container body 1 (container portion 15) from the outside of the cell storage container A40 through the transparent thin portion 26.
  • the lid 2 has a thick portion 25 ′, and the thick portion 25 ′ protrudes downward (the other side in the first direction) inside the container body 1. According to such a configuration, the storage space for the culture medium and the like in the container body 1 is reduced. Therefore, it is possible to reduce the amount of medium used in maintaining and transporting the culture state.
  • the flange 23 is provided with a convex portion 234. According to such a configuration, it is convenient when the cell storage container A40 is stored and transported in another storage container. At the time of transportation of the cell storage container A40, for example, the container body 1 and the lid 2 are sandwiched from above and below by the container member and the lid member of the storage container.
  • the plane P2 formed by the tip 234a of the convex portion 234 is substantially parallel to the major surface 23a of the flange 23.
  • the convex portion 234 does not form a closed annular shape in the vertical direction view (view in the thickness direction of the flange 23).
  • the container body 1 (container portion 15) can be maintained and transported in a culture state under a predetermined gas environment while maintaining the ventilation state with the outside (the internal space of the storage container).
  • each part of the cell storage container according to the present invention can be varied in design in many ways.
  • the cell storage container according to the present invention is not limited to the type shown in the above embodiment, and can be applied to containers such as centrifuge tubes and various culture containers.

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Abstract

Le problème décrit par la présente invention est de fournir un conteneur de cellules convenant au transport, les conditions de culture étant maintenues. La solution selon l'invention porte sur un récipient de cellules muni : d'un corps de récipient (1) comprenant une première section cylindrique (11) présentant une ouverture (110) à son extrémité supérieure ; et un corps de couvercle (2) pour fermer l'ouverture (110), le corps de couvercle (2) comprenant un premier élément de couvercle (2A) ayant une section de plaque supérieure (21) et une seconde section cylindrique (22) qui est ajustée sur l'extérieur de la première section cylindrique (11), et un second élément de couvercle souple (2B) ayant une collerette (23) qui présente une grande épaisseur et une section fine (26) qui présente une petite épaisseur et qui rend étanche le côté interne de la collerette (23), le second élément de couvercle (2B) est maintenu par le premier élément de couvercle (2A) dans un état où la collerette (23) chevauche la section de plaque supérieure (21) telle que visualisée dans le sens de l'épaisseur de la section de plaque supérieure (21), la section fine (26) chevauche avec un trou traversant (211) entouré par la section de plaque supérieure (21) telle que visualisée dans le sens de l'épaisseur de la section de plaque supérieure (21), et le corps de conteneur (1) et le premier élément de couvercle (2A) sont munis d'un moyen de verrouillage pour empêcher le mouvement relatif du corps de récipient (1) et le premier élément de couvercle (2A) dans un état où la collerette (23) est interposée entre la partie périphérique de l'ouverture (110) dans le corps de récipient (1) et la section de plaque supérieure (21) du premier élément de couvercle (2A).
PCT/JP2017/038304 2017-10-24 2017-10-24 Conteneur de cellules WO2019082261A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2019549707A JP7064247B2 (ja) 2017-10-24 2017-10-24 細胞収容容器
PCT/JP2017/038304 WO2019082261A1 (fr) 2017-10-24 2017-10-24 Conteneur de cellules

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PCT/JP2017/038304 WO2019082261A1 (fr) 2017-10-24 2017-10-24 Conteneur de cellules

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020110062A (ja) * 2019-01-09 2020-07-27 株式会社アイカムス・ラボ アダプタ、クローズドチャンバ、細胞培養装置及びアダプタの製造方法
WO2022190261A1 (fr) * 2021-03-10 2022-09-15 株式会社サンプラテック Récipient de culture cellulaire
WO2023033135A1 (fr) * 2021-09-06 2023-03-09 真一 高崎 Équipement de culture pour micro-organismes, équipement de culture contenant un composant de milieu pour des micro-organismes, et procédé de mesure de nombre de micro-organismes l'utilisant
JP7383638B2 (ja) 2018-12-18 2023-11-20 テルモ株式会社 挟持具

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JP7383638B2 (ja) 2018-12-18 2023-11-20 テルモ株式会社 挟持具
JP2020110062A (ja) * 2019-01-09 2020-07-27 株式会社アイカムス・ラボ アダプタ、クローズドチャンバ、細胞培養装置及びアダプタの製造方法
JP7262042B2 (ja) 2019-01-09 2023-04-21 株式会社アイカムス・ラボ アダプタ、クローズドチャンバ、細胞培養装置及びアダプタの製造方法
WO2022190261A1 (fr) * 2021-03-10 2022-09-15 株式会社サンプラテック Récipient de culture cellulaire
WO2023033135A1 (fr) * 2021-09-06 2023-03-09 真一 高崎 Équipement de culture pour micro-organismes, équipement de culture contenant un composant de milieu pour des micro-organismes, et procédé de mesure de nombre de micro-organismes l'utilisant

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