WO2019012622A1 - Dispositif de production d'une structure cellulaire tridimensionnelle ayant une forme arbitraire, et son procédé de production - Google Patents

Dispositif de production d'une structure cellulaire tridimensionnelle ayant une forme arbitraire, et son procédé de production Download PDF

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Publication number
WO2019012622A1
WO2019012622A1 PCT/JP2017/025400 JP2017025400W WO2019012622A1 WO 2019012622 A1 WO2019012622 A1 WO 2019012622A1 JP 2017025400 W JP2017025400 W JP 2017025400W WO 2019012622 A1 WO2019012622 A1 WO 2019012622A1
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Prior art keywords
manufacturing apparatus
linear members
cell structure
space
cell
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PCT/JP2017/025400
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English (en)
Japanese (ja)
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次郎 大野
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次郎 大野
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Priority to PCT/JP2017/025400 priority Critical patent/WO2019012622A1/fr
Publication of WO2019012622A1 publication Critical patent/WO2019012622A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus

Definitions

  • the present invention relates to a method for producing a three-dimensional cell structure or a three-dimensional cell construct using a cell aggregate, and in particular, a three-dimensional cell using a strip-like, linear or planar member formed into an arbitrary shape.
  • the first is the preparation of artificial tissues and organs intended for human transplantation.
  • Artificial tissue is produced in such a way that a portion of the artificial tissue expresses a function, and human transplantation is the final purpose.
  • appropriate evaluation accreditation is required, and long-term efforts are required.
  • the second is a method of utilizing toxicity test, drug effect determination, pathology determination, developmental science, etc. using these three-dimensional cell structures as a test strip.
  • suspension cells There are two types of cells: suspension cells and anchorage-dependent adherent cells.
  • cells of blood system and immune system belong, and in the latter, cells of organs, skin, bone and the like belong.
  • Adherent cells can not survive for a long time in the floating state in a solution, and need to survive and proliferate by adhering to a scaffold such as a glass petri dish or hydrogel.
  • the adherent cells When the adherent cells are placed in a non-adherent environment, the cells adhere to each other in search of a scaffold, cell aggregates are formed, and cell aggregates are placed in an environment in which they are mutually contacted in some way. They adhere and fuse to form larger three-dimensional cell structures. This phenomenon is widely known, and Non-patent documents 1 to 6 show these specific examples.
  • Non-Patent Document 6 shows an idea of treating a three-dimensional cell structure as a "building block", and suggests that various cells can be used.
  • a cell mass is a roughly circular aggregate composed of cells alone
  • a cell aggregate is a aggregate composed of cell mass and cells and other substances. .
  • Patent Document 1 discloses a method for producing a tissue plug which can produce tissue of any shape with cells alone without using a carrier. Specifically, the cell aggregate is placed in a chamber having micropores through which the culture solution can pass only on the bottom surface, and the culture solution is contained in the chamber in an amount such that a part of the cell aggregate contacts the gas phase. The cell aggregates are cultured in a culture solution in an excess amount of the culture solution in the chamber.
  • Patent Document 4 discloses a method of producing a three-dimensional cell by laminating a cultured cell cultured in a flat plane on a permeable sheet on a cultured cell in another planar culture together with the sheet.
  • Patent No. 4122280 U.S. Patent No. 8852932 Patent No. 4517125 International Application No. PCT / JP2008 / 056268
  • International Publication WO2005 / 047496 PLOS ONE Journal. Pone. 0136681
  • a rheological mechanism sufficient to explain the kinetics of cell sorting. J Theor Biol. 1972; 37: 43-73.
  • An object of the present invention is to solve such conventional problems, and to provide a manufacturing apparatus capable of manufacturing a three-dimensional cell structure having an arbitrary shape, and a method for manufacturing the same.
  • a member capable of permeating a liquid such as a culture medium and forming a three-dimensional space for holding cell aggregates is prepared, and a plurality of cell aggregates are collected in the space Providing a collection and fusing the plurality of cell aggregates to form a cell structure.
  • a manufacturing apparatus for manufacturing a cell structure includes a first member defining at least a first space, and a device disposed opposite to the first member and defining at least a second space
  • a plurality of cell aggregates can be accommodated in a third space defined by the first space and the second space, having at least two members, and at least both the first member and the second member Is permeable to liquids such as culture media.
  • a three-dimensional space is formed by a member permeable to a liquid such as a culture medium, and a cell aggregate is supplied in the three-dimensional space to manufacture a cell structure.
  • Three-dimensional structures can be manufactured. Furthermore, it becomes possible to freely set the medium supply channel.
  • FIG. 1 (A) is a perspective view showing a schematic configuration of a cell structure manufacturing apparatus according to an embodiment of the present invention
  • FIG. 1 (B) is a view showing an example of connection between linear members and columns.
  • an arbitrary three-dimensional space for holding cell aggregates is formed by a linear member or a porous member or the like, and the three-dimensional space A plurality of cell aggregates are supplied to the inside to produce a three-dimensional cell structure according to a three-dimensional space.
  • the member forming the three-dimensional space is permeable to a liquid such as a culture medium, so that the cell structure in the three-dimensional space can be supplied with the culture medium or the like from all directions.
  • the member forming the three-dimensional space can be formed using a three-dimensional printer.
  • the three-dimensional printer generates a member that defines an arbitrary three-dimensional space based on the three-dimensional data. It should be noted that the scale of the drawings is exaggerated for the purpose of understanding the present invention, and may not necessarily be different from the size of an actual product or the like.
  • FIG. 1 is a view showing a schematic configuration of a cell structure manufacturing apparatus according to a first embodiment of the present invention.
  • the manufacturing apparatus 100 of the cell structure of the present embodiment includes a plurality of linear members 110, a plurality of columns 120 supporting each of the plurality of linear members 110, a plurality of linear members 130, and a plurality of linear members 130. And a plurality of pillars 140 for supporting each of the linear members 130.
  • the plurality of linear members 110 may be of any shape, for example, the plurality of linear members 110 may have a circular or rectangular cross section, and may be two-dimensionally spaced at a predetermined interval substantially parallel to one another. Extends in a straight line. Alternatively, the plurality of linear members 110 may be curved or bent three-dimensionally at regular intervals substantially parallel to one another. Preferably, the plurality of linear members 110 are processed into an arbitrary shape and made of plastic or other soft material so as to facilitate removal from the cell structure.
  • a plurality of linear members 110 arranged two-dimensionally or three-dimensionally, or processed two-dimensionally or three-dimensionally, form a two-dimensional space or three-dimensional space by the envelope surface of the plurality of lines. As defined above, this two-dimensional space or three-dimensional space provides a space for holding cell aggregates.
  • the plurality of linear members 130 are also configured in the same manner as the plurality of linear members 110, and are arranged two-dimensionally or three-dimensionally, or processed two-dimensionally or three-dimensionally,
  • the envelopes of the lines of each of the linear members 130 define a two-dimensional space or a three-dimensional space, and these spaces provide a space for holding cell aggregates.
  • the plurality of linear members 110 define a two-dimensional flat surface by their lines, and the plurality of linear members 130 have convex steps or bends in part thereof. By forming the part, a substantially convex three-dimensional space S is defined.
  • each of the plurality of linear members 110 is not limited to the same shape, and may have different shapes. The same applies to the plurality of linear members 130. Furthermore, the spacing of each of the plurality of linear members 110 is not limited to being uniform, and each spacing may be different. The same applies to the distance between each of the plurality of linear members 130. Thus, the spacing between each of the plurality of linear members 110 and the spacing between each of the plurality of linear members 130 may be different.
  • the plurality of columns 120 are connected to the back side of each of the plurality of linear members 110. That is, on the back surface of one linear member, it is supported by the plurality of columns 120 along the direction in which the line extends, and as a result, on the back surface side of the plurality of linear members 110, the plurality of columns 120 are two-dimensionally arranged.
  • the plurality of columns 140 are connected to the back side of each of the plurality of linear members 130, and the plurality of columns 140 are two-dimensionally arranged on the back side of the plurality of linear members 130. .
  • the plurality of linear members 110 and the plurality of columns 120 are integrally formed by the three-dimensional printer, and the plurality of linear members 130 and the plurality of columns 140 are also integrally formed by the three-dimensional printer.
  • FIGS. 1 (B), (C), and (D) are diagrams showing an example of connection between a support and a linear member.
  • the connection method between the column and the linear member is arbitrary, in the example shown in FIG. 1B, the column 120/140 has a relatively large head 150 and the head 150 is a linear member. It is engaged in the groove on the back side of 110/130.
  • the tip end portion 152 of the support 120/140 has a trapezoidal shape in which it gradually becomes thinner, and the material forming the support 120/140 and the material forming the linear member 110/130 are Differently, both materials are in an easy-to-peel relationship.
  • FIG. 1B the connection method between the column and the linear member
  • the column 120/140 has a relatively large head 150 and the head 150 is a linear member. It is engaged in the groove on the back side of 110/130.
  • the tip end portion 152 of the support 120/140 has a trapezoidal shape in which it gradually becomes thinner, and the material forming the support 120/
  • the tip end portion 154 of the support column 120/140 has a shape that is easily broken from the linear member 110/130 or is made of a material that is easily broken. As will be described later, when the cell structure of a desired shape is produced by the production apparatus 100, the step of separating the columns 120/140 from the linear members 110/130 is included.
  • FIG. 2 is a schematic cross-sectional view for explaining the manufacturing process of the cell structure of the present example.
  • the ends of the plurality of columns 120 are connected to the lower frame 160, and the ends of the plurality of columns 140 are connected to the upper frame 170.
  • the lower frame 160 includes a recess space and accommodates the linear member 110 and the support 130 in the recess space, and similarly, the upper frame 170 includes the linear member 120 and the linear member 120 in the recess space.
  • the post 140 is accommodated.
  • the lower frame 160 and the upper frame 170 have the same size.
  • the lower frame 160 and the plurality of columns 120 are integrally formed by a three-dimensional printer
  • the upper frame 170 and the plurality of columns 140 are integrally formed by a three-dimensional printer.
  • the lower frame 160 and the upper frame 170 are positioned such that the plurality of linear members 110 face the plurality of linear members 130. At this time, the plurality of linear members 110 and the plurality of linear members 130 are in close contact with each other, and a rectangular space S is formed therebetween.
  • the space S is defined by the plurality of linear members 110 and 130, but since there is a fixed gap between the plurality of linear members 110 and 130, the space S is a linear member Exposed to the outside through the gap of
  • a rectangular space S is formed in order to manufacture a rectangular cell structure, and in the case of manufacturing a cell structure having another shape, a space corresponding to that is formed.
  • Cell aggregates are injected into the rectangular space S.
  • Cell aggregates are a collection of multiple cells.
  • the distance D1 between the plurality of linear members 110 located on the lower side is smaller than the distance D2 between the plurality of linear members 130 located on the upper side (D1 ⁇ D2).
  • the average size (particle size) R is in the relationship of D1 ⁇ R ⁇ D2.
  • An opening (not shown) for injecting a cell aggregate is formed in a part of the upper frame 170, and the discharge part of the dispenser is directed into the space S into the cell aggregate solution through the opening. Discharge.
  • the cell aggregates contained in the solution pass through the linear members 130 having a distance D2 larger than the particle diameter R, and are filled in the space S.
  • the assembly including the lower frame 160 and the upper frame 170 is immersed in a vessel containing the culture fluid.
  • an opening through which the culture solution can enter is formed.
  • the assembly may be subjected to constant vibration or oscillation to promote adhesion and fusion.
  • the culture solution is sufficiently supplied from the three-dimensional direction through the gaps between the plurality of linear members 110 and 130 between the plurality of cell aggregates in the space S, as a result, as shown in FIG. 2 (B). As such, adjacent cell aggregates adhere and fuse to form a cell structure T.
  • the lower frame 160 and the upper frame 170 are pulled apart, and the plurality of columns 120, 140 are separated from the plurality of linear members 110, 130.
  • the columns 120 and 140 are structured to be easily separated from the linear members 110 and 130, and the lower frame 160 and the upper frame 170 are separated.
  • the support columns 120 and 140 are separated from the back side of the linear members 110 and 130.
  • the plurality of linear members 110 and 130 can be removed from the cell structure T in the axial direction X to obtain a cell structure as a final product.
  • FIG. 1 Another structural example of the manufacturing apparatus of this embodiment is shown in FIG.
  • through holes 200, 210, 220, and 230 are formed on the main surfaces of the lower frame 160 and the upper frame 170.
  • the shape, number, and size of the through holes are arbitrary.
  • a passage which can access the space S through the through holes 200 and 210 of the lower frame 160 and a passage which can access the space S through the through holes 220 and 230 of the upper frame 170 And are formed.
  • a nutrient solution or a culture solution can be supplied to the cell aggregate in the space S through the through holes 200 to 230.
  • FIG. 3C is a further modification.
  • the separating member 180 does not have to completely shut off the upper and lower spaces, and it is sufficient if the solutions supplied from the upper and lower sides are prevented from being freely mixed without having a barrier.
  • the separating member 180 can be configured, for example, as a rectangular frame, and is fixed, for example, between the end of the linear member and the inner wall of the frame by an adhesive or the like.
  • FIG. 4 shows a further modification of the manufacturing apparatus of this embodiment.
  • the through holes 240 and 250 are formed only in the upper frame 170, and the culture fluid and the nutrient solution are supplied to the inside through the through holes 240, and the through holes 250 are formed.
  • the culture fluid and nutrient solution are discharged to the outside through the By forming such a circulation route, it is possible to supply a fresh culture fluid or the like to the cell aggregate in the space S, or to supply a selected culture fluid or nutrient solution.
  • the culture fluid and the nutrient solution are supplied to the inside through the through holes 260 of the upper frame 170, and the culture fluid and the nutrition through the through holes 270 of the lower frame 160.
  • the liquid may be discharged to the outside.
  • FIG. 5 shows a further modification of the manufacturing apparatus of this embodiment.
  • a planar member 110A is used instead of the plurality of linear members 110.
  • the planar member 110A may be a flat surface as shown in the figure, or may be a surface (a spherical recess or a rectangular recess) defining a three-dimensional space other than this.
  • a plurality of support columns 120 are connected to the back surface side of the planar member 110A in the same manner as described above.
  • FIG. 5B the space S when the planar member 110A is used is exposed to the outside through the gap of the upper linear member 130.
  • FIG. 6 the further modification of the manufacturing apparatus of a present Example is shown.
  • the directions in which the plurality of linear members 110 and the plurality of linear members 130 extend are the same, but in the example shown in FIG. 6, the plurality of linear members 110 and The direction in which the plurality of linear members 130 extend is different, for example, the directions of both are orthogonal to each other.
  • the linear members 110 and the linear members 130 are drawn out in orthogonal directions, so that the cell structure can be removed at the time of removal.
  • the stress generated in the body can be relieved more than in one direction.
  • FIG. 7 shows a further modification of the manufacturing apparatus of this embodiment.
  • the manufacturing apparatus further includes another plurality of linear members 300. That is, the plurality of linear members have a three-layer structure.
  • Each of the plurality of intermediate linear members 300 may have any shape, any number, any spacing, etc., and extend in the same direction as the plurality of linear members 110 and 130 It may extend in different directions.
  • a plurality of columns 310 are connected to both ends of the intermediate linear member 300.
  • the support 310 is formed on either the lower frame 160 or the upper frame 170. It is fixed. When the lower frame 160 and the upper frame 170 are pulled apart, the support 310 is separated from the linear member 300. Then, the linear members 110, 130, and 300 are removed from the cell structure, respectively.
  • An intermediate linear member 300 can be inserted to promote adhesion, fusion of cell aggregates, or inserted to promote the formation of cell structures.
  • FIG. 8 shows a further modification of the manufacturing apparatus of this embodiment.
  • the manufacturing apparatus further includes a core 330.
  • the plurality of linear members 110 define a semi-cylindrical concave space S1 in a portion thereof, and the plurality of linear members 130 define a semi-cylindrical convex space S2 in a portion thereof
  • the two spaces S1 and S2 define a cylindrical space.
  • the core 330 is a cylindrical member having a diameter smaller than the diameter of the cylindrical space of the spaces S1 and S2, and a part of the core 330 is supported in a floating state by the linear members 110 or 130.
  • the cell aggregate was filled with space S1 and S2, and the cell structure was formed. Thereafter, the plurality of linear members 110, 130 are withdrawn, and the cylindrical core 330 is withdrawn in the axial direction.
  • FIG. 8C for example, a hollow cylindrical cell structure such as a blood vessel can be obtained.
  • FIG. 9 the further modification of the manufacturing apparatus of a present Example is shown.
  • the example shown in the figure is a modification of the planar member 110A shown in FIG. 5, that is, a plurality of through holes 340 are formed in the member 110B on the surface.
  • the shape, size, and number of the through holes 340 are arbitrary.
  • FIG. 9B shows an example in which a semicircular recess 350 is formed in the member 110C on the surface, and a plurality of through holes 352 are formed in the recess 350.
  • FIG. 10 shows a further modification of the manufacturing apparatus of this embodiment.
  • the window 360 which can see the inside is formed in a part of the upper side frame 170.
  • the window 360 may be a through hole, or the window 360 may be attached with transparent glass or transparent plastic to seal the internal space.
  • the adhesion state of the cell aggregate inside and the progress of fusion can be visually confirmed through the window 360. If the culture is confirmed to be insufficient, additional nutrient solution can be supplied.
  • FIG. 11 shows a further modification of the manufacturing apparatus of this embodiment.
  • the porous membrane 380 is embossed toward the back surface of the plurality of linear members 120 by the embossing member 370.
  • a membrane 380 having the same shape as the three-dimensional space of the plurality of linear members 120 can be obtained.
  • Membrane 380 can be, for example, decellularized or a biodegradable material such as collagen.
  • a plurality of linear members 120 can be separated from the embossed membrane 380, and the cell aggregate can be filled in the space of the membrane 380 to form a cell structure.
  • the membrane 380 can be a part of the cell structure, so there is no need to remove it.
  • the step of removing the plurality of linear members 120 can be omitted. , Production of cell structures can be facilitated.
  • the detachment mechanism of the present embodiment includes a grip 400 and a plurality of connecting members 410.
  • One end of the connecting member 410 is connected to the grip 400, and the other end is connected to the back surface side of the plurality of linear members 110, and the connecting members 410A on both sides are connected to the end of the upper frame 170A Be done.
  • the through-hole for making the some connection member 410 penetrate is formed in lower side frame 160A.
  • the lower frame 160A is pulled away from the upper frame 170A by applying a force to the grip 400 so that the gap between the grip 400 and the lower frame 160A is narrowed, and at the same time, the plurality of columns 120 are a plurality of lines Are separated from the back surface of the second member 110.
  • the detachment mechanism further includes a grip 420 and a plurality of connection members 430.
  • One end of the connection member 430 is connected to the grip 420 and the other end is connected to the back side of the plurality of linear members 130.
  • the through-hole for making the some connection member 410 penetrate is formed in the upper side frame 170A.
  • the plurality of columns 140 are separated from the back surface of the plurality of linear members 130 by applying a force to the grip 420 so that the distance between the grip 420 and the upper frame 170A is narrowed.
  • FIG. 13 is a view for explaining an example of extraction of a plurality of linear members from the cell structure T.
  • the linear member 500 is made of a soft material so that it can be easily removed even if it has an arbitrary shape, and at the same time, the strength that the linear member 500 does not break is required.
  • FIG. 13 (C) is a single-core linear member
  • FIG. 13 (D) is an example of a multi-core linear member. When removing such a linear member, the linear member can be removed while rotating so as to facilitate removal from the cell structure.
  • FIG. 14 is a block diagram showing the electrical configuration of the cell aggregate production apparatus of this example.
  • the manufacturing apparatus of this embodiment includes a supply source 610 for supplying a culture solution, a nutrient solution, and the like to the assembly 600 of the lower frame 160 and the upper frame 170, and an assembly from the supply source 610.
  • the controller 690 includes, for example, a RAM / ROM, a microprocessor or the like, and preferably controls each unit by executing a program that controls the manufacturing process of the cell aggregate.
  • FIG. 15 shows an example of a control sequence of the manufacturing process by the controller 690.
  • the assembly 600 is a stack of the lower frame 160 and the upper frame 170, and the inner spaces of both frames are filled with cell aggregates.
  • the supply source 610 is connected to the through hole 260 (see FIG. 4C) of the upper frame 170 by a pipe for conveying a fluid, and the discharge source 640 is penetrated by the lower frame 160 by a pipe. It is connected to the hole 270. From such a state, production of a cell structure shall be started.
  • the internal temperature of the assembly 600 is detected by the temperature sensor 670 (S100), and the internal pressure of the assembly 600 is detected by the pressure sensor 680 (S102).
  • the controller 690 controls the flow rate of fluid supplied to the assembly 600 based on the detected temperature and pressure via the valve control unit 630 and controls the flow rate of fluid discharged from the assembly 600 via the valve control unit 660 (S104). For example, when the pressure of the assembly 600 is above a certain value, the flow rate of the supplied fluid may be decreased or the flow rate of the discharged fluid may be increased. Also, when the temperature in the assembly 600 is above a certain value, the flow rate of the supplied fluid is increased, or the flow rate of the discharged fluid is decreased.
  • the controller 690 checks whether or not a predetermined time has elapsed (S106), and if it has not, the steps S100 to S104 are repeated.
  • the fixed time is, for example, a time until cell aggregates adhere and fuse to form a cell structure.
  • FIG. 14 shows an example in which the fluid is supplied from one source 610 to the assembly 600
  • a plurality of sources are connected to the assembly 600 through a plurality of pipes, and a plurality of sources are connected.
  • the valve may be controlled to supply the assembly 600 with a fluid selected from the following sources. For example, at the first temperature, the first type of fluid is supplied to the assembly 600, and at the second temperature, the supply of the first type of fluid is stopped, and the second type of fluid is assembled to the assembly. It may be supplied. Alternatively, during the first time period, the first type of fluid is supplied to the assembly 600, and during the second time period, the supply of the first type of fluid is stopped, and the second type of Fluid may be supplied to the assembly.

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Abstract

Le problème décrit par la présente invention est de fournir un dispositif de production d'une structure cellulaire tridimensionnelle ayant une forme arbitraire. [Solution] Un dispositif de production destiné à produire une structure cellulaire selon la présente invention comprend : de multiples éléments linéaires 110 ; de multiples supports en colonne 120 pour supporter les multiples éléments linéaires 110 ; de multiples éléments linéaires 130 ; de multiples supports en colonne 140 pour supporter les multiples éléments linéaires 130 ; un corps de cadre inférieur 160A qui est relié aux supports en colonne 120 ; un corps de cadre supérieur 170A relié aux supports en colonne 140 ; un élément de préhension 400 pour détacher les multiples supports en colonne 120 des multiples éléments linéaires 110 ; et un élément de préhension 420 pour détacher les multiples supports en colonne 140 des multiples éléments linéaires 130.
PCT/JP2017/025400 2017-07-12 2017-07-12 Dispositif de production d'une structure cellulaire tridimensionnelle ayant une forme arbitraire, et son procédé de production WO2019012622A1 (fr)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7471579B1 (ja) 2023-04-28 2024-04-22 ティシューバイネット株式会社 立体細胞構造体を製造する方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5602028A (en) * 1995-06-30 1997-02-11 The University Of British Columbia System for growing multi-layered cell cultures
JP2007515958A (ja) * 2003-12-19 2007-06-21 ユニヴァーシティー オブ ウォータールー 培養細胞、細胞培養の方法および機器
WO2012111684A1 (fr) * 2011-02-15 2012-08-23 国立大学法人佐賀大学 Dispositif débulleur dans un robot de manipulation de cellules automatisée
JP2013005751A (ja) * 2011-06-24 2013-01-10 Saga Univ 細胞の立体構造体製造装置
JP2016000029A (ja) * 2014-05-20 2016-01-07 国立大学法人佐賀大学 組織体形成装置及び組織体形成キット
JP2016054655A (ja) * 2014-09-05 2016-04-21 日本写真印刷株式会社 培養容器
WO2016068292A1 (fr) * 2014-10-31 2016-05-06 富士フイルム株式会社 Structure tubulaire, dispositif de fabrication de structure tubulaire, et procédé de fabrication de structure tubulaire
JP2017023049A (ja) * 2015-07-22 2017-02-02 株式会社ファンケル 観察窓部を有する細胞培養容器、細胞培養装置及び培養細胞の側面からの観察方法

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5602028A (en) * 1995-06-30 1997-02-11 The University Of British Columbia System for growing multi-layered cell cultures
JP2007515958A (ja) * 2003-12-19 2007-06-21 ユニヴァーシティー オブ ウォータールー 培養細胞、細胞培養の方法および機器
WO2012111684A1 (fr) * 2011-02-15 2012-08-23 国立大学法人佐賀大学 Dispositif débulleur dans un robot de manipulation de cellules automatisée
JP2013005751A (ja) * 2011-06-24 2013-01-10 Saga Univ 細胞の立体構造体製造装置
JP2016000029A (ja) * 2014-05-20 2016-01-07 国立大学法人佐賀大学 組織体形成装置及び組織体形成キット
JP2016054655A (ja) * 2014-09-05 2016-04-21 日本写真印刷株式会社 培養容器
WO2016068292A1 (fr) * 2014-10-31 2016-05-06 富士フイルム株式会社 Structure tubulaire, dispositif de fabrication de structure tubulaire, et procédé de fabrication de structure tubulaire
JP2017023049A (ja) * 2015-07-22 2017-02-02 株式会社ファンケル 観察窓部を有する細胞培養容器、細胞培養装置及び培養細胞の側面からの観察方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7471579B1 (ja) 2023-04-28 2024-04-22 ティシューバイネット株式会社 立体細胞構造体を製造する方法

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