WO2017214823A1

WO2017214823A1 - Reagent kit having cold insulation, temperature display, alarming, and sample adding reminding functions

Info

Publication number: WO2017214823A1
Application number: PCT/CN2016/085627
Authority: WO
Inventors: 石庆学
Original assignee: 石庆学
Priority date: 2016-06-13
Filing date: 2016-06-13
Publication date: 2017-12-21

Abstract

A reagent kit having cold insulation, temperature display, alarming and sample adding reminding functions. By arranging sound production devices (40), a control unit and a storage battery on a reagent kit body (10), and arranging a refrigeration device (381) and a temperature control probe (382) on the outer walls of the reagent holes (38), a needed precooling environment (2-8ºC) can be provided to the reagent, the temperature can be detected by means of the temperature control probe (382), and is presented experiment personnel by means of a display device (80). Once the temperature exceeds a preset range, the sound production devices (40) immediately give alarms to remind the experiment personnel, thereby reducing the probability of an experiment failure; in addition, a reagent tray (36) drives the reagent holes (38) to rotate, so that the experiment personnel can be reminded of adding samples, thereby avoiding experiment failure caused by sample adding errors.

Description

Title of Invention: Kit with cold preservation, temperature display and alarm, and sample reminder function

[0001] The present invention relates to the field of biotechnology, and more particularly to a kit having a cold retention, temperature display and alarm, and a sample reminder function.

Background technique

[0002] In the field of biotechnology, in addition to technical breakthroughs, it is more important to improve the efficiency of research and experimentation. Among them, the emergence of kits plays an important role. The kit standardizes the steps of common biological experiments, providing the experimenter with the reagents to be prepared and the optimized loading method, which greatly increases the speed of the experimental development and the degree of standardization of the results.

technical problem

[0003] Due to the biological characteristics, many reagents in the use of hydrazine to ensure the effectiveness of the reagents and the accuracy of the experiment, need to be completed in a low temperature (2-8 ° C) environment, and currently, such operations are usually used " The ice box is "completed, the ice cubes are added to the appropriate size of the foam box, and the reagent tube is inserted into the ice to ensure that the reagents are at the desired low temperature. However, because many biological experiments require aseptic processing, and ice cubes are easily stained with bacteria in the air, which is a potential source of bacterial contamination in experiments, low-temperature loading operations using "ice boxes" are used in these experiments.吋 The requirements for the operation of the experimenter are higher, the burden on the experimenter is increased, and the experimental success rate cannot be guaranteed. Another disadvantage of using an "ice box" is that it is not possible to ensure that the reagent tube inserted in it is in the desired temperature environment (2-8 ° C). When the temperature is outside this range, the experimenter is not immediately aware of the situation and may have a certain impact on the experimental results.

[0004] In addition, as the complexity of the experiment is increasing, the number of reagents in the existing kits is also increasing, and the loading step is becoming more and more complicated. Take the commonly used real-time PCR kit as an example. The general kit contains Taq enzyme, buffer, chromogenic dye and sterile ultrapure water. In addition, the reagents required by the experimenter include at least the upstream primer. Downstream primers and template, a total of 7 reagents together to prepare a fluorescent quantitative PCR reaction system. In many cases, the experimenter will have the phenomenon of missing, adding, adding, etc. during the process of adding samples. The reason is that there are many kinds of reagents, and the process of loading cannot be well identified and marked.吋 Handling other urgent matters and causing the ongoing experiment to be interrupted, the error is in the office Inevitably.

Problem solution

Technical solution

In order to solve the above problems in the prior art, the present invention provides a kit having a cold retention, temperature display and alarm, and a sample reminder function.

[0006] The technical solution adopted by the present invention is: a kit with cold preservation, temperature display and alarm, and sample reminding function, comprising a kit body and a lid, wherein the kit body is provided with a sounding device and a control Unit, small battery and display unit. The sounding device, the control unit and the battery are electrically connected; the display device, the control unit and the battery are electrically connected; the outer wall of the reagent hole is provided with a refrigeration device and a temperature control probe, and the refrigeration device and the temperature control probe Electrical connection with the control unit.

[0007] The inner layer of the kit body is further provided with a lining plate, and the lining plate is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicator light is further disposed between the two. The lining plate is spaced apart from the mouth of the box body such that the lining plate forms a clogging space with the box body; the reagent rotating disk is provided with a plurality of reagent holes, And connected to a power mechanism.

[0008] the reagent box body is further provided with an inductor, the sensor is disposed corresponding to a bottom of the pipette tip head collecting hole, and the sensor receives the hole by sensing the pipette tip head The bottom controls the rotation of the reagent rotating disk and the color of the arrow indicator light.

Further, the kit body has a two-layer structure, and the control unit, the sounding device and the small power storage battery are disposed between the inner layer and the outer layer of the reagent cartridge body.

[0010] Further, the number of the reagent wells is six.

[0011] Further, the display device is a digital display screen.

[0012] Further, the sounding device is a buzzer, and the number is two.

[0013] Further, the control unit is a single chip microcomputer.

[0014] Further, the small battery is a lithium ion battery.

[0015] Further, the inductor is connected to the bottom of the pipette tip collecting hole by a spring.

[0016] Further, the reagent rotating disk is provided with 8 reagent holes; the power mechanism connected thereto is a stepping motor, and is connected to the inductor and controlled by the inductor.

Further, the arrow indicator material is an LED, connected to the sensor, and controlled by the sensor Advantageous effects of the invention

Beneficial effect

[0018] The present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device. Once the setting range is exceeded, the sounding device immediately alerts the experimenter to reduce the probability of failure of the experiment; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding the wrong sample. The experiment failed.

Brief description of the drawing

DRAWINGS

1 is an overall configuration diagram of the present invention.

2 is a structural diagram of the reagent well.

3 is a schematic view showing the structure of a reagent rotating disk, an arrow indicator lamp, and a pipette tip head collecting hole as shown in FIG. 1. A preferred embodiment for carrying out the invention.

BEST MODE FOR CARRYING OUT THE INVENTION

[0022] The present invention will be further described below in conjunction with the accompanying drawings.

[0023] As shown in FIG. 1, a kit having a cold preservation, temperature display and alarm, and a sample reminding function includes a kit body 10 and a lid 20, and the kit body is provided with a liner 30 and sounding The device 40 and a control unit (not shown), a small battery (not shown), and a display device 80. The sounding device, the control unit and the battery are electrically connected; the display device, the control unit and the battery are electrically connected.

[0024] As shown in FIG. 2, the outer wall of the reagent hole is provided with a refrigerating device 381 and a temperature control probe 382, and the refrigerating device, the temperature control probe and the control unit are electrically connected.

[0025] As shown in FIG. 3, the lining plate 30 is provided with a pipette tip collecting hole 32 and a reagent rotating disk 36, and an arrow indicator light 34 is further disposed between the two, and the reagent rotating disk 36 is disposed on the reagent rotating plate 36. Several reagent wells 38. Sensor 60 Placed in the casing 10, the inductor 60 is disposed corresponding to the bottom of the pipette tip collecting hole 32, and the inductor 60 controls the stepping motor 70 to drive the reagent rotating disk through the bottom of the inductive pipette tip collecting hole 32. The rotation of 36 and the color of the arrow indicator light 34 are illuminated. With the above arrangement, the inductor 60 is connected to the bottom of the pipette tip collecting hole 32 by the spring 50. When the loading operation is completed, the discarded tip is driven into the inductive pipette tip head collecting hole 32, and the sensor 60 controls the stepping motor 70 to drive the reagent rotation by inducing the pipette tip to collect the bottom of the hole 32. The color of the disk 36 is rotated and the arrow indicator light 34 is illuminated. The color of the arrow indicator light 34 is in one-to-one correspondence with the reagent holes on the reagent rotating disk 36. The eight colors of red, orange, yellow, green, cyan, blue, purple, and white correspond to 1-8 on the reagent rotating disk 36, respectively. Hole. Each time the sensor senses that the discarded tip is driven into the pipette tip collection hole, the control stepper motor 70 drives the reagent rotating disk 36 to rotate 45° to its next reagent hole, and controls the arrow indicator. 34 lights up the color of the corresponding reagent hole.

[0026] The present invention provides a required pre-cooling environment (2-8 ° C) for a reagent by providing a sounding device, a control unit, and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device. Once the setting range is exceeded, the sounding device immediately alerts the experimenter to reduce the probability of failure of the experiment; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding the wrong sample. The experiment failed.

[0027] The present invention is not limited to the above-described preferred embodiments, and any other form of product can be derived by anyone of the present invention, but without any change in its shape or structure, The same or similar technical solutions are all within the scope of the present invention.

Industrial applicability

[0028] The present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device. Once the setting range is exceeded, the sounding device immediately alerts the experimenter to reduce the probability of failure of the experiment; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding the wrong sample. The experiment failed.

Claims

Claim

A kit with cold preservation, temperature display and alarm, and sample reminding function, comprising a kit body, wherein the kit body is provided with a lining plate, a plurality of reagent holes, a sounding device, a control unit, a small battery and Display device. The sounding device, the control unit and the battery are electrically connected.

A kit for cold storage, temperature display and alarm, and sample reminding function according to claim 1, wherein said sound generating device is a buzzer.

A kit for cold storage, temperature display and alarm, and sample reminder function according to claim 1, wherein said display device is a digital display.

A kit with cold storage, temperature display and alarm, and sample reminding function according to claim 1, wherein the kit body has a two-layer structure, and the control unit, the sounding device and the small battery are disposed at The kit is between the inner layer and the outer layer. A kit with cold storage, temperature display and alarm, and sample reminding function according to claim 1, wherein the liner is provided with a pipette tip collecting hole and a reagent rotating disk, both of which are There is also an arrow indicator. The lining plate is spaced apart from the mouth of the box body such that the lining plate forms a clogging space with the box body; the reagent rotating disk is provided with a plurality of reagent holes, And connected to a power mechanism; a reagent tube disposed in the reagent hole;

a sensor disposed in the casing, the inductor being disposed corresponding to a bottom of the pipette tip collecting hole, and the inductor controlling the bottom of the collecting hole by sensing the pipe tip The power mechanism drives the rotation of the reagent rotating disk and the color of the arrow indicator light.

The kit of claim 1 , wherein the arrow indicator is made of an LED, wherein the arrow indicator is The sensors are connected, and light of eight colors of red, orange, yellow, green, cyan, blue, purple, and white can be respectively illuminated as needed.