CN210065787U - Medical instrument for quickly diagnosing pathogens - Google Patents

Medical instrument for quickly diagnosing pathogens Download PDF

Info

Publication number
CN210065787U
CN210065787U CN201920617537.0U CN201920617537U CN210065787U CN 210065787 U CN210065787 U CN 210065787U CN 201920617537 U CN201920617537 U CN 201920617537U CN 210065787 U CN210065787 U CN 210065787U
Authority
CN
China
Prior art keywords
heat preservation
box body
heat
test
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201920617537.0U
Other languages
Chinese (zh)
Inventor
景北和
刘蔚东
吴昊翔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangsu Sanhe Bioengineering Engineering Co Ltd
Original Assignee
Jiangsu Sanhe Bioengineering Engineering Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangsu Sanhe Bioengineering Engineering Co Ltd filed Critical Jiangsu Sanhe Bioengineering Engineering Co Ltd
Priority to CN201920617537.0U priority Critical patent/CN210065787U/en
Application granted granted Critical
Publication of CN210065787U publication Critical patent/CN210065787U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Landscapes

  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The utility model relates to a medical instrument for pathogen rapid diagnosis, including a box body and with the box cover that the box body articulated formed, have a chamber, a test chamber that keep distributing side by side in the box body, and keep keeping through the heat preservation baffle between chamber and the test chamber and keeping, it has a partition hole to open at the middle part of heat preservation baffle, is provided with respectively in chamber and the test chamber and keeps heat preservation, experimental heat preservation, it is provided with quick positioning mechanism to keep between heat preservation, experimental heat preservation and the box body. The utility model has the advantages that: through set up the partition hole on the thermal insulation baffle, then because save chamber and test chamber and will cool down respectively and preserve and the intensification experiment, the temperature of thermal insulation baffle both sides exists very big difference promptly, and the setting of partition hole is in order to separate thermal insulation baffle from the centre, avoids both sides temperature to influence each other, ensures going on smoothly of cooling down and intensification.

Description

Medical instrument for quickly diagnosing pathogens
Technical Field
The utility model relates to a medical device, in particular to a medical instrument for rapid pathogen diagnosis.
Background
A pathogen for human and animal diseases, Listeria monocytogenes, which can cause Listeria infectious diseases of human and animals, is mainly manifested by symptoms of meningitis, septicemia and abortion, and has a wide range of Listeria monocytogenes. The method has the advantages that the method is detected in various foods, has low requirement on environmental temperature, can survive at low temperature, and therefore refrigerated foods are also detected mostly, in recent years, Listeria monocytogenes infection events occur frequently in various countries, even cause casualties, and are widely concerned by various social circles, at present, molecular biology detection technologies based on nucleic acid amplification, such as RTPCR, PCR, real-time fluorescence quantitative PCR and the like, play a great role in diagnosis and detection of animal epidemic diseases, but the methods have high requirement on proficiency and professional level of operators, need accurate temperature control equipment and accurate and expensive analysis instruments, are long in time consumption, are not suitable for field detection or large-area use in a basic level, and can detect pathogenic microorganisms accurately and quickly in an early stage.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to provide a medical instrument for pathogen rapid diagnosis, which can detect pathogenic microorganisms early, accurately and rapidly.
In order to solve the technical problem, the utility model adopts the technical scheme that: a medical apparatus for rapid pathogen diagnosis is characterized in that: comprises that
The box body and the box cover hinged with the box body are provided with a storage cavity and a test cavity which are distributed in parallel, the storage cavity is isolated from the test cavity through a heat-insulating partition plate, a separation hole is formed in the middle of the heat-insulating partition plate, a storage heat-insulating layer and a test heat-insulating layer are respectively arranged in the storage cavity and the test cavity, and quick positioning mechanisms are arranged among the storage heat-insulating layer, the test heat-insulating layer and the box body;
a cold insulation device is arranged in the storage cavity, cold insulation agent is filled in the cold insulation device, and a plurality of accommodating holes are formed in the top end of the cold insulation device;
the test chamber is internally provided with a reagent tube placing plate, the heating tube is arranged below the reagent tube placing plate, a reflecting film is coated on the surface of the heat preservation layer below the heating tube, the side surface of the reagent tube placing plate and the heat preservation layer of the side wall of the test chamber are spirally arranged to form heating wires, the reagent tube placing plate is provided with a plurality of reagent tubes for placing holes, the inner wall of each reagent tube placing hole is provided with a temperature sensor, the reagent tube placing plate is provided with a controller, the temperature sensors are electrically connected with the controllers, and the controllers are electrically connected with the heating wires and the heating tube.
Further, the other end of box body and lid is fixed through a locking mechanism, locking mechanism is: the side of the box body is provided with a lock tongue seat, the side of the box cover is provided with a lock core, and the lock core is provided with a lock tongue matched with the lock tongue seat.
Further, the quick positioning mechanism is: be provided with in the bottom of box body and preserve the heat preservation, experimental heat preservation corresponding location is protruding, all has simultaneously in the bottom of preserving heat preservation, experimental heat preservation with the protruding corresponding positioning groove in location, is provided with several location sand grip at the side of box body, has the constant head tank with location sand grip looks one-to-one simultaneously at the side of preserving heat preservation, experimental heat preservation.
Furthermore, the top of the containing hole is provided with a shockproof ring which is a sponge layer made of sponge.
The utility model has the advantages that: adopt the heat preservation baffle to divide into the low temperature that is fit for the transportation to preserve the chamber with the box body cavity and be fit for the constant temperature test chamber of witnessed inspections, realized presenting the constant temperature environment of low temperature and certain temperature in a box body, the test chamber places the board heat conduction and reflection film reflection heating pipe heat through the reagent pipe and maintains control test chamber constancy of temperature, guarantees stability and the rate of accuracy of detection.
Through set up the partition hole on the thermal insulation baffle, then because save chamber and test chamber and will cool down respectively and preserve and the intensification experiment, the temperature of thermal insulation baffle both sides exists very big difference promptly, and the setting of partition hole is in order to separate thermal insulation baffle from the centre, avoids both sides temperature to influence each other, ensures going on smoothly of cooling down and intensification.
To locking mechanism's setting, then utilize lock tongue seat, lock core, spring bolt overlap joint complex mode, lock box body and lid, avoid in the transportation, reagent pipe drops from the box body.
Quick positioning mechanism's setting to realized preserving the heat preservation, experimental heat preservation and the box body between quick location and the relative fixed of mutual position, avoided at the in-process that removes, preserve heat preservation, experimental heat preservation and box body striking each other, also made things convenient for follow-up quick replacement heat preservation.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Fig. 1 is a schematic view of a medical device for rapid pathogen diagnosis according to the present invention.
Fig. 2 is the interior schematic view of the test cavity of the present invention.
Fig. 3 is a schematic view of the locking mechanism of the present invention.
Detailed Description
The following examples are presented to enable those skilled in the art to more fully understand the present invention and are not intended to limit the scope of the present invention.
A medical device for rapid pathogen diagnosis as shown in FIGS. 1-3 comprises
A box body 2 and box cover 1 that forms with the box body is articulated, a save chamber 3 that distributes side by side has in the box body 2, a test chamber 9, and keep keeping apart through thermal baffle 4 between chamber 3 and the test chamber 9, it has one to separate hole 41 to open at thermal baffle 4's middle part, through setting up on thermal baffle 4 and separating hole 41, then be because save chamber 3 and test chamber 9 will cool down respectively and preserve and the intensification experiment, there is very big difference in the temperature of thermal baffle 4 both sides promptly, and separate the setting of hole 41, then separate thermal baffle 4 from the middle in order to be with thermal baffle 4, avoid both sides temperature to influence each other, ensure cooling and the going on smoothly of intensification.
The other end of box body 2 and lid 1 is fixed through a locking mechanism, and locking mechanism is: a lock tongue seat 23 is arranged at the side end of the box body 2, a lock core 25 is arranged at the side end of the box cover 1, and a lock tongue 24 matched with the lock tongue seat 23 is arranged on the lock core 25. For the arrangement of the locking mechanism, the box body and the box cover are locked by using a mode that the bolt seat 23, the lock core 25 and the bolt 24 are in lap joint and matched, so that the reagent tube is prevented from falling from the box body 2 in the transportation process.
It preserves heat preservation 31, experimental heat preservation 91 to be provided with respectively in preserving chamber 3 and experimental chamber 9, preserves and is provided with quick positioning mechanism between heat preservation 31, the experimental heat preservation 91 and the box body 2. In this embodiment, vacuum heat insulating materials are used for the heat insulating partition plate 4, the storage heat insulating layer 31, and the test heat insulating layer 91.
The quick positioning mechanism is as follows: be provided with and preserve heat preservation 31 in the bottom of box body 2, it is protruding that experimental heat preservation 91 is corresponding to fix a position, simultaneously in preserving heat preservation 31, experimental heat preservation 91's bottom all has with the protruding corresponding positioning groove 21 in location, be provided with several location sand grip at the side of box body 2, simultaneously in preserving heat preservation 31, experimental heat preservation 91's side has the constant head tank 22 with location sand grip looks one-to-one, and constant head tank 22 extends to the bottom of preserving heat preservation 31 or experimental heat preservation 91. Quick positioning mechanism's setting, thereby realized preserving heat preservation 31, the relative fixation of the quick location between experimental heat preservation 91 and the box body 2 and position each other, avoid at the in-process that removes, preserve heat preservation 31, experimental heat preservation 91 strikes with box body 2 each other, also make things convenient for follow-up quick replacement heat preservation, and adopt the protruding cooperation with the location sand grip of side in the location of bottom, thereby realize fixing preserving heat preservation 31, experimental heat preservation 91 from three direction.
A cold insulation device 6 is arranged in the storage cavity 3, cold insulation agent is filled in the cold insulation device 6, a plurality of containing holes 7 are formed in the top end of the cold insulation device 6, shockproof rings 8 are arranged at the tops of the containing holes 7, and the shockproof rings 8 are sponge layers made of sponge.
The accommodating hole 7 is respectively provided with a DEPC water pipe, a BstDAN polymerase pipe, an L.M positive control pipe, a fluorescent fuel pipe, a Listeria monocytogenes LAMP detection mixed liquid 1 pipe, a Listeria monocytogenes LAMP detection mixed liquid 2 pipe and a Listeria monocytogenes LAMP detection mixed liquid 3 pipe.
As shown in fig. 2, a reagent tube placing plate 10 is provided in the test chamber 9, the reagent tube placing plate 10 is a good conductor of heat, a heating pipe 13 is provided below the reagent tube placing plate 10, a reflective film 14 is coated on the surface of a heat insulating layer 4 below the heating pipe 13, heating wires 12 are spirally arranged between the side surface of the reagent tube placing plate 10 and the heat insulating layer 4 on the side wall of the test chamber 9, four reagent tube placing holes 11 are provided on the reagent tube placing plate 10, a temperature sensor 15 is provided on the inner wall of the reagent tube placing hole 11, a controller 16 is provided on the reagent tube placing plate 11, the temperature sensor 15 is electrically connected with the controller 16, and the controller 16 is electrically connected with the heating wires 12 and the heating pipe 13.
When in use, the REX-C100 type controller 16 is utilized to adjust the temperature in the test cavity 9 to be maintained at 63 ℃, reagent tubes are respectively placed in the reagent tube placing holes 11, the labels are 1, 2 and 3 … …, 2 mu L of Listeria monocytogenes LAPM detection mixed solution 1 is added into the reagent tubes of the label 1 and the label 2, 3.5 mu L of Listeria monocytogenes LAPM detection mixed solution 2 is added, 12.5 mu L of Listeria monocytogenes LAPM detection mixed solution 3, 1 mu L of Bst DAN polymerase and 1 mu L of corresponding fluorescent dye are added, the added DEPC water is controlled to be 23 mu L, after the mixture is evenly mixed, 2 mu L of corresponding sample DNA to be detected is added into the reagent tube of the label 1, L of M positive reference substance is added into the reagent tube of the label 2, other reagent tubes of the label 3 and the like can also be added with different samples to be detected, the box cover 1 is covered, after the constant temperature amplification reaction is carried out for 45min at 63 ℃, and (4) observing whether the color of the LAMP reaction solution is obviously changed, wherein the LAMP reaction solution is positive if the color is changed into green, and is negative if the color is orange under the opposite condition.
It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (4)

1. A medical device for rapid diagnosis of pathogens, characterized by: comprises that
The box body and the box cover hinged with the box body are provided with a storage cavity and a test cavity which are distributed in parallel, the storage cavity is isolated from the test cavity through a heat-insulating partition plate, a separation hole is formed in the middle of the heat-insulating partition plate, a storage heat-insulating layer and a test heat-insulating layer are respectively arranged in the storage cavity and the test cavity, and quick positioning mechanisms are arranged among the storage heat-insulating layer, the test heat-insulating layer and the box body;
a cold insulation device is arranged in the storage cavity, cold insulation agent is filled in the cold insulation device, and a plurality of accommodating holes are formed in the top end of the cold insulation device;
the test chamber is internally provided with a reagent tube placing plate, the heating tube is arranged below the reagent tube placing plate, a reflecting film is coated on the surface of the heat preservation layer below the heating tube, the side surface of the reagent tube placing plate and the heat preservation layer of the side wall of the test chamber are spirally arranged to form heating wires, the reagent tube placing plate is provided with a plurality of reagent tubes for placing holes, the inner wall of each reagent tube placing hole is provided with a temperature sensor, the reagent tube placing plate is provided with a controller, the temperature sensors are electrically connected with the controllers, and the controllers are electrically connected with the heating wires and the heating tube.
2. The medical device for the rapid diagnosis of pathogens according to claim 1, characterized in that: the other end of box body and lid is fixed through a locking mechanism, locking mechanism is: the side of the box body is provided with a lock tongue seat, the side of the box cover is provided with a lock core, and the lock core is provided with a lock tongue matched with the lock tongue seat.
3. The medical device for the rapid diagnosis of pathogens according to claim 1, characterized in that: the quick positioning mechanism is as follows: be provided with in the bottom of box body and preserve the heat preservation, experimental heat preservation corresponding location is protruding, all has simultaneously in the bottom of preserving heat preservation, experimental heat preservation with the protruding corresponding positioning groove in location, is provided with several location sand grip at the side of box body, has the constant head tank with location sand grip looks one-to-one simultaneously at the side of preserving heat preservation, experimental heat preservation.
4. The medical device for the rapid diagnosis of pathogens according to claim 1, characterized in that: the top of the containing hole is provided with a shockproof ring which is a sponge layer made of sponge.
CN201920617537.0U 2019-04-30 2019-04-30 Medical instrument for quickly diagnosing pathogens Expired - Fee Related CN210065787U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201920617537.0U CN210065787U (en) 2019-04-30 2019-04-30 Medical instrument for quickly diagnosing pathogens

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201920617537.0U CN210065787U (en) 2019-04-30 2019-04-30 Medical instrument for quickly diagnosing pathogens

Publications (1)

Publication Number Publication Date
CN210065787U true CN210065787U (en) 2020-02-14

Family

ID=69450069

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201920617537.0U Expired - Fee Related CN210065787U (en) 2019-04-30 2019-04-30 Medical instrument for quickly diagnosing pathogens

Country Status (1)

Country Link
CN (1) CN210065787U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109971642A (en) * 2019-04-30 2019-07-05 江苏三和生物工程股份有限公司 A kind of medical instrument for pathogen quick diagnosis

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109971642A (en) * 2019-04-30 2019-07-05 江苏三和生物工程股份有限公司 A kind of medical instrument for pathogen quick diagnosis

Similar Documents

Publication Publication Date Title
US5366873A (en) Device and method for use in detecting microorganisms in a sample
CN102405402A (en) Droplet-based assay system
ES2327381T1 (en) AUTOMATED DIAGNOSTIC ANALYZER AND PROCEDURE.
CN210065787U (en) Medical instrument for quickly diagnosing pathogens
Cano et al. Fluorescent detection‐polymerase chain reaction (FD‐PCR) assay on microwell plates as a screening test for salmonellas in foods
CN206087669U (en) Pseudomonas fluorescens detect reagent box
CN115704049A (en) Nucleic acid detection method and detection reactor
CN205886956U (en) Flow cytometry test -tube rack
CN104862216A (en) High-throughput visual totally-enclosed split-type LAMP-LFD detection chip device
US10323266B2 (en) Antimicrobial susceptibility testing device, antimicrobial susceptibility testing kit, and antimicrobial susceptibility testing method
CN203728843U (en) Split type LAMP-LFD (loop-mediated isothermal amplification-lateral flow dipstick) chip detection device with characteristics of high throughput, visualization and full closing
CN208776736U (en) A kind of pathogen constant temperature quick detection kit
Stöcher et al. Automated detection of five human herpes virus DNAs by a set of LightCycler PCRs complemented with a single multiple internal control
Stöcher et al. A simple approach to the generation of heterologous competitive internal controls for real-time PCR assays on the LightCycler
CN208532801U (en) The relevant Epstein-Barr virus detection kit of cancer
Weiss et al. [16] Mass screening for mutants with altered DNases by microassay techniques
CN216525873U (en) Refrigeration structure and analysis appearance of reagent dish
CN208043782U (en) A kind of kit of detection
CN107760598A (en) A kind of portable constant temperature nucleic acid augmentative instrument
CN104488851B (en) A kind of full-automatic multi-step sequence frigorimeter
CN102719548B (en) Kit for detecting brucella and use method thereof
US11525115B2 (en) Process for the isolation and analysis of microorganisms contained in a sample
CN203569116U (en) Multiple (Polymerase Chain Reaction) detection kit
CN102605069B (en) Enterohemorrhagic Escherichia coli O104: H4 detection kit and use method thereof
CN109971642A (en) A kind of medical instrument for pathogen quick diagnosis

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20200214

Termination date: 20210430

CF01 Termination of patent right due to non-payment of annual fee