CN208532801U - The relevant Epstein-Barr virus detection kit of cancer - Google Patents
The relevant Epstein-Barr virus detection kit of cancer Download PDFInfo
- Publication number
- CN208532801U CN208532801U CN201820840703.9U CN201820840703U CN208532801U CN 208532801 U CN208532801 U CN 208532801U CN 201820840703 U CN201820840703 U CN 201820840703U CN 208532801 U CN208532801 U CN 208532801U
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- barr virus
- epstein
- neto
- reagent
- reagent tube
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Abstract
The utility model provides a kind of cancer relevant Epstein-Barr virus detection kit, which includes shell, inner cavity and Neto.Neto is movably set in inner cavity by pulley and two sliding rails, and Neto is arranged with eight detection shrinkage pools of 2 × 4 array arrangements, wherein being inserted with Reagent Tube in seven detection shrinkage pools, electronic tag is posted in the outside of each Reagent Tube.Reagent Tube includes the reaction reagent pipe equipped with RT-PCR reaction solution, the enzymatic reagent pipe equipped with enzyme mixation, the negative control reagent pipe equipped with Epstein-Barr virus feminine gender quality-control product, the positive control agent pipe equipped with Epstein-Barr virus positive quality control product, distilled water Reagent Tube, the ROX Reagent Tube equipped with ROX correcting fluid and primed probe liquid Reagent Tube equipped with sterilizing distilled water.Semiconductor temperature-control device is installed in the lumen and below Neto, for the environment temperature of inner cavity to be adjusted to Epstein-Barr virus detection required temperature.The utility model can fast and accurately detect Epstein-Barr virus, high specificity, high sensitivity, stabilization, conveniently.
Description
Technical field
The utility model relates to the technical field of Epstein-Barr virus detection more particularly to a kind of relevant Epstein-Barr virus detection examinations of cancer
Agent box.
Background technique
Epstein-Barr virus belongs to the DNA virus of the thermophilic lymphocyte virus of nerpes vinrus hominis γ subfamily, reaches in population of China infection rate
90% or more, it can be involved each organ of whole body and system；Epstein-Barr virus has infects the mankind and certain spirits in specific manner in vivo and in vitro
The biological characteristics of long class B cell；People is the host of ebv infection, main to be propagated by saliva；Epstein-Barr virus is on pars oralis pharyngis
Proliferation, then infects bone-marrow-derived lymphocyte, these cells largely enter blood circulation and cause systemic infection, and can in chrotoplast
It is hidden in human lymphoid's tissue for a long time；EBV infection can behave as proliferative infection and latent infection；Different Infection Status tables
Up to different antigen, the antigen of proliferative infection phase expression has EBV early antigens, EBV capsid protein and EBV membranous antigen, hides
The infection phase antigen of expression has EBV nuclear antigen and latent membrane protein；The virus infection diverse clinical manifestations, and be easy to fail to pinpoint a disease in diagnosis, miss
It examines.
Polymerase chain reaction (PCR) technology is widely used to animal epidemic detection, which is mainly based upon cause of disease
The specificity of microbial nucleic acids carries out exponential amplification to DNA or RNA segment for drone design specific primer；Therefore, it applies
Fluorescence PCR assay establishes Epstein-Barr virus detection method and has great importance for fast and accurately detecting Epstein-Barr virus.It is external at present
The kit of the detection EBV-DNA listed have it is several, the method for detection be mainly fluorescence immunoassay hybridization (HIGH), the country inspection
It surveys EBV and mainly uses ELISA method, there are no the kit of approval listing detection EBV-DNA.It is such based on EBV infection conditions
Seriously, developing simple, easy, the lower-cost detection kit of one kind is just particularly important.Existing kit is deposited mostly
Complex steps are being used, the problems such as detection reagent is mutually polluted, is difficult to differentiate between Reagent Tube, and because of the examination in kit
Agent storage temperature cannot save state in constant temperature and influence Epstein-Barr virus detection accuracy, and sensitivity is lower.
Utility model content
The main purpose of the utility model is to provide a kind of relevant Epstein-Barr virus detection kits of cancer, it is intended to solve existing
Have because the reagent storage temperature in kit cannot be in the problem of influencing Epstein-Barr virus detection accuracy due to constant temperature saves state.
To achieve the above object, the utility model provides a kind of cancer relevant Epstein-Barr virus detection kit, including shell,
Inner cavity and Neto, which is characterized in that it is described it is interior it is intracavitary be provided with two sliding rails, four angles bottom of the Neto is respectively set
One pulley, the Neto are movably set in inner cavity by the pulley and two sliding rails, be provided in the Neto with 2 ×
Eight detection shrinkage pools of 4 array arrangements are inserted with Reagent Tube wherein having in seven detection shrinkage pools, another detection shrinkage pool is spare
Hole, in which:
The Reagent Tube includes the reaction reagent pipe equipped with RT-PCR reaction solution, the enzymatic reagent pipe equipped with enzyme mixation, dress
There is the negative control reagent pipe of Epstein-Barr virus feminine gender quality-control product, the positive control agent pipe equipped with Epstein-Barr virus positive quality control product, be equipped with
Distilled water Reagent Tube, the ROX Reagent Tube equipped with ROX correcting fluid and the primed probe liquid Reagent Tube of sterilizing distilled water；
Electronic tag is posted in the outside of each Reagent Tube, for distinguishing the Epstein-Barr virus detection reagent in each Reagent Tube；
The adjustable semiconductor temperature-control device of temperature, the semiconductor temperature are installed in the inner cavity and below Neto
The power supply line input terminal for controlling device is electrically connected battery, which is used to for interior intracavitary environment temperature to be adjusted to EB disease
Poison detection required temperature is to realize that reagent is kept the temperature.
Preferably, the aperture for being inserted with the detection shrinkage pool of the reaction reagent pipe of the RT-PCR reaction solution is greater than other seven inspections
The aperture of shrinkage pool is surveyed, the pore size of other seven detections shrinkage pool is equal.
Preferably, the inner cavity is additionally provided with driver, and the power supply line input terminal of the driver is electrically connected to battery, institute
It states battery and provides driving electric energy for driver.
Preferably, the driver includes driving motor and electrically driven (operated) free extension bar, and the one of the free extension bar
End is mechanically attached to the side of Neto, and the other end of the free extension bar is electrically connected to driving motor, when the driving motor drives
Dynamic free extension bar drives Neto free to slide in the inner cavity along two sliding rails when stretching up and down.
Preferably, the shell of driver connection Neto opposite face side offers sliding slot, the end positions of the sliding slot
Match with the end positions of two sliding rails, the height of the sliding slot is greater than the thickness of Neto, and the length of the sliding slot is greater than interior
The width of support.
Preferably, the surface of the shell is provided with export button and warehouse entry button, the export button and warehouse entry button
It is electrically connected to the control terminal of the driver.
Preferably, the enzyme mixation that the enzymatic reagent pipe is provided with is reverse transcriptase and amplification enzyme.
Preferably, the inner wall of the shell is coated with thermal insulation layer.
Compared to the prior art, the relevant Epstein-Barr virus detection kit of cancer described in the utility model uses above-mentioned technical side
Case obtains following technical effect: it can fast and accurately detect cancer correlation Epstein-Barr virus, high specificity, and high sensitivity, surely
It is fixed, conveniently, it will not be polluted between detection reagent, and accurate detection can be made to Epstein-Barr virus；It coats and protects in reagent inboard wall of cartridge
Adiabator, and Neto is rigid foam；In addition, being convenient for reagent box body quilt by placing semiconductor temperature-control device in kit body
When tyre, it can guarantee that the intracorporal temperature of reagent of kit is in constant temperature and saves state, avoid due to temperature is more than storage temperature
Influence detection accuracy.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the relevant Epstein-Barr virus detection kit of the utility model cancer.
The embodiments will be further described with reference to the accompanying drawings for the realization, functional characteristics and advantage of the utility model aim.
Specific embodiment
Further to illustrate that the utility model is the technical means and efficacy reaching predetermined purpose of utility model and being taken,
Below in conjunction with attached drawing and preferred embodiment, to specific embodiment of the present utility model, structure, feature and its effect, specifically
It is bright as follows.It should be appreciated that specific embodiment described herein is only used to explain the utility model, it is not used to limit this reality
With novel.
Shown in referring to Fig.1, Fig. 1 is the structural schematic diagram of the relevant Epstein-Barr virus detection kit of the utility model cancer.?
In the present embodiment, the relevant Epstein-Barr virus detection kit of the cancer includes reagent box body 10, and the reagent box body 10 is pull
The rectangular shape of formula structure, the reagent box body 10 include shell 1, inner cavity 2 and Neto 3.The inner wall of the shell 1 is coated with
Thermal insulation layer 11, the thermal insulation layer 11 are made of thermal insulation material, realize reagent heat preservation, improve Epstein-Barr virus detection accuracy, avoid Yin Wen
Degree factor influences accuracy in detection.
As a preferred embodiment, two sliding rails 12 are provided in the inner cavity 2, the Neto 3 is rectangular parallelepiped structure, in institute
A pulley 13 is respectively set in four angles bottom of support 3, each pulley 13 can be with free to slide on sliding rail 12.The Neto 3
It is movably set in inner cavity 2 by the pulley 13 and two sliding rails 12.Be arranged between two sliding rails 12 in inner cavity 2 away from
From the equal length with Neto 3, so as to so that Neto 3 is existed by the pulley 13 that four angles bottom is arranged along two sliding rails 12
It is free to slide in inner cavity 2.The inner cavity 2 is additionally provided with driver 14 and battery 15, the power supply line input of the driver 14
End is electrically connected to battery 15, and the battery 15 is that driver 14 provides driving electric energy.The driver 14 includes driving electricity
Machine 140 and electrically driven (operated) free extension bar 141, one end of the free extension bar 141 are mechanically attached to the side of Neto 3, separately
One end is electrically connected to driving motor 140, drives Neto 3 when the driving motor 140 about 141 free extension bar of driving is flexible
It is free to slide in inner cavity 2 along two sliding rails 12.
As a preferred embodiment, the Neto 3 is made of rigid foam, is provided in the Neto 3 with 2 × 4 arrays row
Eight detection shrinkage pools of cloth wherein have in seven detection shrinkage pools among eight detection shrinkage pools and are inserted with Reagent Tube；The Reagent Tube packet
Include the reaction reagent pipe 31 equipped with Epstein-Barr virus RT-PCR reaction solution, the enzymatic reagent pipe 32 equipped with enzyme mixation, equipped with Epstein-Barr virus yin
Property the negative control reagent pipe 33 of quality-control product, the positive control agent pipe 34 equipped with Epstein-Barr virus positive quality control product, double equipped with sterilizing
Steam distilled water Reagent Tube 35, the ROX Reagent Tube 36 equipped with ROX correcting fluid and the primed probe liquid Reagent Tube 37 of water.Other one
A detection shrinkage pool be spare hole 38, be inserted with the reaction reagent pipe 31 of RT-PCR reaction solution detection shrinkage pool aperture be greater than other seven
The aperture of a detection shrinkage pool, the pore size of other seven detection shrinkage pools is equal, posts electronic tag outside each Reagent Tube,
For distinguishing the Epstein-Barr virus detection reagent in each Reagent Tube.
In the present embodiment, the Epstein-Barr virus RT-PCR reaction solution that the reaction reagent pipe 31 is equipped be used for by sample to be tested into
Row PCR reaction configuration Epstein-Barr virus reaction solution, the enzyme mixation that the enzymatic reagent pipe 32 is equipped with is reverse transcriptase and amplification enzyme, is used
In extracting viral nucleic acid (RNA) from Epstein-Barr virus reaction solution, and viral RNA amplification is carried out.The negative control reagent pipe 33 fills
The Epstein-Barr virus positive quality control product that some Epstein-Barr virus feminine gender quality-control products and positive control agent pipe 34 are equipped with can be from sample to be tested
Detect Epstein-Barr virus nucleic acid or its nucleic acid content.The sterilizing distilled water that the distilled water Reagent Tube 35 is equipped with is for configuring Epstein-Barr virus
The concentration of reaction solution, the ROX correcting fluid that the ROX Reagent Tube 36 is equipped with are used for the concentration to amendment Epstein-Barr virus reaction solution,
The primed probe liquid Reagent Tube 37 is for drawing sample to be tested.
As preferred embodiment, each Reagent Tube is the screw socket glass tube of sterilizing, in the inner cavity 2 and is located at
The lower section of Neto 3 is equipped with the adjustable semiconductor temperature-control device 4 of temperature, and the power supply line input terminal of the semiconductor temperature-control device 4 is electrically connected
Battery 15 is connect, temperature needed for which environment temperature in inner cavity 2 can be adjusted to Epstein-Barr virus detection
It is interior, it realizes reagent heat preservation, improves Epstein-Barr virus detection accuracy, avoid influencing accuracy in detection because of temperature factor.
As preferred embodiment, the shell 1 that the driver 14 connects 3 opposite face side of Neto offers sliding slot
16, the end positions of the end positions of the sliding slot 16 and two sliding rails 12 match, and the height of sliding slot 16 is greater than the thickness of Neto 3
Degree, length are greater than the width of Neto 3, and therefore, Neto 3 can skid off outside reagent box body 10 through sliding slot 16 from inner cavity 2.In this reality
It applies in example, 1 surface of shell of the reagent box body 10 is provided with export button 17 and warehouse entry button 18,17 He of export button
Warehouse entry button 18 is electrically connected to the control terminal of the driver 14.When tester presses export button 17, driver 14 can
It is slided out to outside reagent box body 10, is added in Reagent Tube convenient for tester to be measured molten from inner cavity 2 with driving Neto 3 to pass through sliding slot 16
Liquid (such as saliva or sweat etc.)；When tester adds solution to be measured in Reagent Tube to be finished and press warehouse entry button 18,
Neto 3 can be passed through sliding slot 16 along two sliding rails 12 and promoted in inner cavity 2 automatically by driver 14.
The relevant Epstein-Barr virus detection kit of cancer described in the utility model can fast and accurately detect cancer correlation
Epstein-Barr virus, high specificity, high sensitivity are stablized, conveniently, will not be polluted between detection reagent, and can make standard to Epstein-Barr virus
Really detection；Thermal insulation material is coated in reagent inboard wall of cartridge, and Neto is rigid foam；In addition, by being placed in kit body
Semiconductor temperature-control device when convenient for reagent box body by tyre, can guarantee that the intracorporal temperature of reagent of kit is in constant temperature and saves shape
State avoids influencing detection accuracy because temperature is more than storage temperature.
The above is only the preferred embodiments of the utility model, and therefore it does not limit the scope of the patent of the utility model, all
Equivalent structure or equivalent flow shift made based on the specification and figures of the utility model, is applied directly or indirectly in
Other related technical areas are also included in the patent protection scope of the utility model.
Claims (5)
1. a kind of relevant Epstein-Barr virus detection kit of cancer, including shell, inner cavity and Neto, which is characterized in that in described
Intracavitary to be provided with two sliding rails, a pulley is respectively set in four angles bottom of the Neto, and the Neto passes through the pulley
It is movably set in inner cavity with two sliding rails, 5 eight detection shrinkage pools being provided with 2 × 4 array arrangements in the Neto,
In have in seven detection shrinkage pools and be inserted with Reagent Tube, another detection shrinkage pool is spare hole, in which:
The Reagent Tube includes the reaction reagent pipe equipped with RT-PCR reaction solution, the enzymatic reagent pipe equipped with enzyme mixation, equipped with EB
The negative control reagent pipe of viral feminine gender quality-control product, is equipped with sterilizing at the positive control agent pipe equipped with Epstein-Barr virus positive quality control product
The distilled water Reagent Tube of distilled water, the ROX Reagent Tube and primed probe liquid Reagent Tube that 10 liquid are corrected equipped with ROX；
Electronic tag is posted in the outside of each Reagent Tube, for distinguishing the Epstein-Barr virus detection reagent in each Reagent Tube；
The adjustable semiconductor temperature-control device of temperature, the semiconductor temperature-control device are installed in the inner cavity and below Neto
Power supply line input terminal be electrically connected battery, the semiconductor temperature-control device be used for by interior intracavitary 15 environment temperature be adjusted to EB disease
Poison detection required temperature is to realize that reagent is kept the temperature.
2. the relevant Epstein-Barr virus detection kit of cancer as described in claim 1, which is characterized in that it is anti-to be inserted with the RT-PCR
The aperture of the detection shrinkage pool of the reaction reagent pipe of liquid is answered to be greater than the aperture of other seven detection shrinkage pools, other seven detections are recessed
The pore size in hole is equal.
3. the relevant Epstein-Barr virus detection kit of cancer as described in claim 1, which is characterized in that the inner cavity is additionally provided with
Driver, the power supply line input terminal of the driver are electrically connected to battery, and the battery provides driving electric energy for driver.
4. the relevant Epstein-Barr virus detection kit of cancer as claimed in claim 3, which is characterized in that the driver includes driving
Dynamic motor and electrically driven (operated) free extension bar, one end of the free extension bar are mechanically attached to the side of Neto, this is freely stretched
The other end of contracting bar is electrically connected to driving motor, drives Neto edge when driving motor driving free extension bar is flexible up and down
Two sliding rails it is free to slide in the inner cavity.
5. the relevant Epstein-Barr virus detection kit of cancer as claimed in claim 3, which is characterized in that in the driver connection
The shell of support opposite face side offers sliding slot, and the end positions of the end positions of the sliding slot and two sliding rails match, described
The height of sliding slot is greater than the thickness of Neto, and the length of the sliding slot is greater than the width of Neto.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820840703.9U CN208532801U (en) | 2018-05-31 | 2018-05-31 | The relevant Epstein-Barr virus detection kit of cancer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820840703.9U CN208532801U (en) | 2018-05-31 | 2018-05-31 | The relevant Epstein-Barr virus detection kit of cancer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208532801U true CN208532801U (en) | 2019-02-22 |
Family
ID=65382047
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820840703.9U Expired - Fee Related CN208532801U (en) | 2018-05-31 | 2018-05-31 | The relevant Epstein-Barr virus detection kit of cancer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208532801U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019228449A1 (en) * | 2018-05-31 | 2019-12-05 | 深圳市圣必智科技开发有限公司 | Cancer-related eb virus nucleic acid content measurement kit and method |
| CN112407608A (en) * | 2020-11-26 | 2021-02-26 | 玉林市动物疫病预防控制中心 | LAMP (loop-mediated isothermal amplification) detection auxiliary device for duck circovirus |
-
2018
- 2018-05-31 CN CN201820840703.9U patent/CN208532801U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019228449A1 (en) * | 2018-05-31 | 2019-12-05 | 深圳市圣必智科技开发有限公司 | Cancer-related eb virus nucleic acid content measurement kit and method |
| CN112407608A (en) * | 2020-11-26 | 2021-02-26 | 玉林市动物疫病预防控制中心 | LAMP (loop-mediated isothermal amplification) detection auxiliary device for duck circovirus |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190222 Termination date: 20210531 |