CN109971642A - A kind of medical instrument for pathogen quick diagnosis - Google Patents
A kind of medical instrument for pathogen quick diagnosis Download PDFInfo
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- CN109971642A CN109971642A CN201910362508.9A CN201910362508A CN109971642A CN 109971642 A CN109971642 A CN 109971642A CN 201910362508 A CN201910362508 A CN 201910362508A CN 109971642 A CN109971642 A CN 109971642A
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- insulating layer
- box body
- test
- chamber
- thermal
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
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- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of medical instruments for pathogen quick diagnosis, including a box body and with box body it is hinged made of box cover, have in the box body and the one of column distribution saves chamber, a test cavity, and it saves and is isolated between chamber and test cavity by thermal-insulation baffle plate, a separation hole is provided in the middle part of thermal-insulation baffle plate, insulating layer, test insulating layer are saved saving to be respectively arranged in chamber and test cavity, is provided with quick positioning mechanism between the preservation insulating layer, test insulating layer and box body.The present invention has the advantages that separating hole by being arranged on thermal-insulation baffle plate, it is then to carry out cooling preservation and elevated temperature test due to saving chamber and test cavity respectively, i.e. there is very big difference in the temperature of thermal-insulation baffle plate two sides, and separate the setting in hole, it is then to separate thermal-insulation baffle plate from centre, two sides temperature is avoided to influence each other, it is ensured that cool down and go on smoothly with what is heated up.
Description
Technical field
The present invention relates to a kind of Medical Devices, specifically a kind of medical instrument for pathogen quick diagnosis.
Background technique
A kind of infecting both domestic animals and human encephalapthy agent, Listeria monocytogenes can cause the Listeria infectious diseases of humans and animals,
It is mainly shown as the symptoms such as meningitis, septicemia and miscarriage, Listeria monocytogenes existence range is wide.Examined in a variety of foods
It measures, it is not high to ambient temperature requirements, it can survive under low temperature, therefore chilled food also has detection more, in recent years, various countries' frequency has
Listeria monocytogenes infect event, or even cause many casualties, by various circles of society's extensive concern, currently,
RTPCR, PCR, real-time fluorescence quantitative PCR etc. are diagnosed and are examined in animal epidemic based on the molecular Biological Detection technology of nucleic acid amplification
It surveys aspect and plays great function, but these methods are more demanding to the proficiency and professional standards of operator, and need
Accurate Temperature-controlled appliance and accurate expensive analysis instrument are unsuitable for carrying out on-site test or big face in base with duration
Product uses, and carries out early stage to pathogenic microorganism, quickly and accurately detects.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of pair of pathogenic microorganisms to carry out early stage, the accurate use quickly detected
In the medical instrument of pathogen quick diagnosis.
In order to solve the above technical problems, the technical solution of the present invention is as follows: a kind of Medical treatment device for pathogen quick diagnosis
Tool, innovative point are: including
One box body and with box body it is hinged made of box cover, have in the box body and the one of column distribution save chamber, a test cavity,
And save and be isolated between chamber and test cavity by thermal-insulation baffle plate, a separation hole is provided in the middle part of thermal-insulation baffle plate, in preservation chamber and
It is respectively arranged in test cavity and saves insulating layer, test insulating layer, set between the preservation insulating layer, test insulating layer and box body
It is equipped with quick positioning mechanism;
The preservation is intracavitary to be provided with cold-keeping device, cold-retaining agent is housed inside cold-keeping device, the top of cold-keeping device is provided with
Several accommodating holes;
It is provided with Reagent Tube placement plate in the test cavity, heating tube, the guarantor below heating tube are set below Reagent Tube placement plate
Warm layer surface covers reflectance coating, the heat preservation interlayer helical arrangement setting heater strip of Reagent Tube placement plate side and test cavity side wall, examination
Several Reagent Tube putting holes are set in agent pipe placement plate, temperature sensor are set on the inner wall of Reagent Tube putting hole, Reagent Tube is put
It sets and controller is set on plate, temperature sensor is electrically connected with the controller, and controller is electrically connected with heater strip and heating tube.
Further, the other end of the box body and box cover is fixed by a retaining mechanism, the retaining mechanism are as follows: in box
The side of body is equipped with lock tongue, is equipped with lock core in the side of box cover, and the lock tongue with lock tongue cooperation is provided on lock core.
Further, the quick positioning mechanism are as follows: be provided in the bottom end of box body and save insulating layer, test insulating layer
Corresponding positioning protrusion, at the same save insulating layer, test insulating layer bottom end all have it is corresponding with positioning protrusion fixed
Position groove is provided with several positioning convex strips in the side of box body, at the same save insulating layer, test insulating layer side have with
The mutually one-to-one locating slot of positioning convex strip.
Further, bump protection ring is provided at the top of the accommodating hole, bump protection ring is spongy layer made of sponge.
The present invention has the advantages that using thermal-insulation baffle plate by box body chamber be divided into be suitble to transport save cryo-conservation chamber and
It is suitble to the constant temperature test chamber of on-site test, realizes the isoperibol that low temperature and certain temperature are presented in a box body, test
Chamber is thermally conductive by Reagent Tube placement plate and reflectance coating reflection heating tubular heat maintains Control experiment chamber temperature constant, guarantees detection
Stability and accuracy rate.
Separate hole by being arranged on thermal-insulation baffle plate, then be to carry out due to saving chamber and test cavity respectively cooling preservation and
There is very big difference in elevated temperature test, the i.e. temperature of thermal-insulation baffle plate two sides, and separate the setting in hole, then is in order to by thermal-insulation baffle plate
It is separated from centre, two sides temperature is avoided to influence each other, it is ensured that cooled down and gone on smoothly with what is heated up.
Setting for retaining mechanism, then be by lock tongue, lock core, lock tongue overlap joint cooperation in the way of, to box body and box
Lid is locked, and is avoided during transportation, Reagent Tube is fallen from box body.
The setting of quick positioning mechanism, thus realize save insulating layer, test insulating layer and box body between it is quick calmly
Position and each other position it is relatively fixed, avoid in the process of moving, save insulating layer, test insulating layer and box body it is mutual
It hits, also facilitates subsequent quick-replaceable insulating layer.
Detailed description of the invention
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Fig. 1 is the schematic diagram of the medical instrument for pathogen quick diagnosis of the invention.
Fig. 2 is to test intracavitary portion's schematic diagram in the present invention.
Fig. 3 is the schematic diagram of retaining mechanism in the present invention.
Specific embodiment
The following examples can make professional and technical personnel that the present invention be more fully understood, but therefore not send out this
It is bright to be limited among the embodiment described range.
A kind of medical instrument for pathogen quick diagnosis as shown in FIG. 1 to FIG. 3, including
One box body 2 and with box body it is hinged made of box cover 1, have in box body 2 and the one of column distribution save chamber 3, a test cavity
9, and save and be isolated between chamber 3 and test cavity 9 by thermal-insulation baffle plate 4, it is provided with a separation hole 41 at the middle part of thermal-insulation baffle plate 4, is led to
It crosses the setting on thermal-insulation baffle plate 4 and separates hole 41, be then to carry out cooling preservation and heating due to saving chamber 3 and test cavity 9 respectively
There is very big difference in test, the i.e. temperature of 4 two sides of thermal-insulation baffle plate, and separate the setting in hole 41, then is in order to by thermal-insulation baffle plate 4
It is separated from centre, two sides temperature is avoided to influence each other, it is ensured that cooled down and gone on smoothly with what is heated up.
Box body 2 and the other end of box cover 1 are fixed by a retaining mechanism, retaining mechanism are as follows: are equipped in the side of box body 2
Lock tongue 23 is equipped with lock core 25 in the side of box cover 1, and the lock tongue 24 cooperated with lock tongue 23 is provided on lock core 25.For
The setting of retaining mechanism, then be by lock tongue 23, lock core 25, the overlap joint cooperation of lock tongue 24 in the way of, box body and box cover are carried out
Locking, avoids during transportation, Reagent Tube is fallen from box body 2.
Insulating layer 31, test insulating layer 91 are saved saving to be respectively arranged in chamber 3 and test cavity 9, preservation insulating layer 31,
Quick positioning mechanism is provided between test insulating layer 91 and box body 2.In the present embodiment, thermal-insulation baffle plate 4, preservation are kept the temperature
Layer 31, the test tool of insulating layer 91 use vacuum heat insulation material.
Quick positioning mechanism are as follows: be provided in the bottom end of box body 2 corresponding with insulating layer 31, test insulating layer 91 is saved
Positioning protrusion, at the same save insulating layer 31, test insulating layer 91 bottom end all have it is corresponding with positioning protrusion positioning it is recessed
Slot 21 is provided with several positioning convex strips in the side of box body 2, while having in the side for saving insulating layer 31, testing insulating layer 91
Have with positioning convex strip mutually one-to-one locating slot 22, and locating slot 22 extends to and saves insulating layer 31 or test insulating layer 91
Bottom end.The setting of quick positioning mechanism, to realize quick between preservation insulating layer 31, test insulating layer 91 and box body 2
Positioning and each other position it is relatively fixed, avoid in the process of moving, saving insulating layer 31, test insulating layer 91 and box
Body 2 is mutually hit, and also facilitates subsequent quick-replaceable insulating layer, and using the positioning convex strip of the positioning protrusion and side of bottom end
Cooperation, to realize the fixation to saving insulating layer 31, testing insulating layer 91 from three directions.
It is provided with cold-keeping device 6 saving in chamber 3, cold-retaining agent, the top of cold-keeping device 6 are housed inside cold-keeping device 6
Several accommodating holes 7 are provided with, bump protection ring 8 is provided at the top of accommodating hole 7, bump protection ring 8 is spongy layer made of sponge.
DEPC water pipe, BstDAN polymerase pipe, L.M positive control pipe, fluorochrome are respectively arranged in accommodating hole 7
Pipe, Listeria monocytogenes LAMP detection mixed liquor 1 pipe, Listeria monocytogenes LAMP detection mixed liquor 2 pipe and Listeria monocytogenes
LAMP detects mixed liquor 3 and manages.
As shown in Fig. 2, being provided with Reagent Tube placement plate 10 in test cavity 9, Reagent Tube placement plate 10 is the good conductor of heat,
Heating tube 13 is set below Reagent Tube placement plate 10, and reflectance coating 14 is covered on 4 surface of insulating layer of 13 lower section of heating tube, and Reagent Tube is placed
Heater strip 12 is arranged in helical arrangement between the insulating layer 4 of 9 side wall of 10 side of plate and test cavity, is arranged four in Reagent Tube placement plate 10
Temperature sensor 15 is arranged on the inner wall of Reagent Tube putting hole 11, control is arranged in Reagent Tube placement plate 11 for Reagent Tube putting hole 11
Device 16 processed, temperature sensor 15 are electrically connected with controller 16, and controller 16 is electrically connected with heater strip 12 and heating tube 13.
In application, adjusting temperature in test cavity 9 using REX-C100 type controller 16 maintains 63 DEG C, Reagent Tube putting hole
A Reagent Tube is respectively placed in 11, and marked as 1,2,3 ..., the mono- increasing Li Si of 2 μ L is added into the Reagent Tube of label 1 and label 2
Special bacterium LAPM detects mixed liquor 1, and 3 .5 μ L Listeria monocytogenes LAPM are added and detect mixed liquor 2,12 mono- increasing Lee of .5 μ L are added
This spy bacterium LAPM detects 3,1 μ L Bst DAN polymerase of mixed liquor, and the 1 corresponding fluorescent dye of μ L, control is so that the DEPC being added
The corresponding measuring samples DNA of 2 μ L is added into the Reagent Tube of label 1, is added into the Reagent Tube of label 2 to 23 μ L for water after mixing
L .M positive reference substance, there are also remaining Reagent Tubes such as label 3, and different samples to be tested can also be added, and covers box cover 1, constant temperature 63
After DEG C amplified reaction 45min, whether observation LAMP reaction solution color occurs significantly changing, if becoming green as the positive, phase
In anti-situation if it is orange then for feminine gender.
It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, above-described embodiment and explanation
It is merely illustrated the principles of the invention described in book, without departing from the spirit and scope of the present invention, the present invention also has
Various changes and modifications, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention
It is defined by the appending claims and its equivalent thereof.
Claims (4)
1. a kind of medical instrument for pathogen quick diagnosis, it is characterised in that: including
One box body and with box body it is hinged made of box cover, have in the box body and the one of column distribution save chamber, a test cavity,
And save and be isolated between chamber and test cavity by thermal-insulation baffle plate, a separation hole is provided in the middle part of thermal-insulation baffle plate, in preservation chamber and
It is respectively arranged in test cavity and saves insulating layer, test insulating layer, set between the preservation insulating layer, test insulating layer and box body
It is equipped with quick positioning mechanism;
The preservation is intracavitary to be provided with cold-keeping device, cold-retaining agent is housed inside cold-keeping device, the top of cold-keeping device is provided with
Several accommodating holes;
It is provided with Reagent Tube placement plate in the test cavity, heating tube, the guarantor below heating tube are set below Reagent Tube placement plate
Warm layer surface covers reflectance coating, the heat preservation interlayer helical arrangement setting heater strip of Reagent Tube placement plate side and test cavity side wall, examination
Several Reagent Tube putting holes are set in agent pipe placement plate, temperature sensor are set on the inner wall of Reagent Tube putting hole, Reagent Tube is put
It sets and controller is set on plate, temperature sensor is electrically connected with the controller, and controller is electrically connected with heater strip and heating tube.
2. the medical instrument according to claim 1 for pathogen quick diagnosis, it is characterised in that: the box body and box
The other end of lid is fixed by a retaining mechanism, the retaining mechanism are as follows: lock tongue is equipped in the side of box body, in box cover
Side is equipped with lock core, and the lock tongue with lock tongue cooperation is provided on lock core.
3. the medical instrument according to claim 1 for pathogen quick diagnosis, it is characterised in that: the quick positioning
Mechanism are as follows: the bottom end of box body is provided with positioning protrusion corresponding with insulating layer, test insulating layer is saved, while being protected saving
Warm layer, the bottom end for testing insulating layer all have positioning groove corresponding with positioning protrusion, are provided in the side of box body several
Positioning convex strip, while having and the mutually one-to-one locating slot of positioning convex strip in the side for saving insulating layer, testing insulating layer.
4. the medical instrument according to claim 1 for pathogen quick diagnosis, it is characterised in that: the accommodating hole top
Portion is provided with bump protection ring, and bump protection ring is spongy layer made of sponge.
Priority Applications (1)
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CN201910362508.9A CN109971642A (en) | 2019-04-30 | 2019-04-30 | A kind of medical instrument for pathogen quick diagnosis |
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CN201910362508.9A CN109971642A (en) | 2019-04-30 | 2019-04-30 | A kind of medical instrument for pathogen quick diagnosis |
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CN207899490U (en) * | 2017-11-28 | 2018-09-25 | 陇东学院 | A kind of bladder cancer genetic detection reagent box |
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CN208562415U (en) * | 2018-06-08 | 2019-03-01 | 江苏省肿瘤医院 | A kind of real-time fluorescence PCR assay kit of Hepcidin-gene |
CN208776736U (en) * | 2018-04-16 | 2019-04-23 | 天津迈基生物科技有限公司 | A kind of pathogen constant temperature quick detection kit |
CN210065787U (en) * | 2019-04-30 | 2020-02-14 | 江苏三和生物工程股份有限公司 | Medical instrument for quickly diagnosing pathogens |
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2019
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US20030153021A1 (en) * | 2001-12-06 | 2003-08-14 | Arbor Vita Corporation | Effective monitoring system for anthrax smallpox, or other pathogens |
JP2007093558A (en) * | 2005-09-30 | 2007-04-12 | Japan Science & Technology Agency | Heat-insulated specimen container, and heat and cold insulated container |
US20140220667A1 (en) * | 2012-08-02 | 2014-08-07 | Mohammadali Safavieh | Device for pathogen detection |
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