CN207130266U - A kind of reused RT PCR quick detection kits - Google Patents
A kind of reused RT PCR quick detection kits Download PDFInfo
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- CN207130266U CN207130266U CN201721061246.5U CN201721061246U CN207130266U CN 207130266 U CN207130266 U CN 207130266U CN 201721061246 U CN201721061246 U CN 201721061246U CN 207130266 U CN207130266 U CN 207130266U
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Abstract
A kind of reused RT PCR quick detection kits, the placement plate of Reagent Tube is carried including box body, lid, polylith, it is characterized in that, the box body is flexibly connected with the side of lid, the restricted retaining structure for placing plate ejection and the elastic construction for promoting placement plate to move up in the box body, retaining structure is arranged at box body inside side walls top, and retaining structure is located at the upside that plate is placed in top side, box body bottom inside is fixed in elastic construction one end, and plate contacts-side-down is placed in one end with lower side；Place and reagent wells are set on plate, the Reagent Tube for holding reagent is placed sequentially in reagent wells, is set on box side wall and is placed plate extraction hole.In use, the placement plate for being had been used up reagent by placing plate extraction hole is extracted out, bullet on elastic construction, place plate for second and move up, and limited by retaining structure, the like, reach a reused effect of RT PCR kits.
Description
Technical field
The utility model belongs to field of biological detection, and in particular to a kind of reused RT-PCR quick detection reagents
Box.
Background technology
RT-PCR is reverse transcription PCR or claims reverse transcription PCR, and RT-PCR is the polymerase chain by RNA reverse transcription and DNA
The technology that formula amplification is combined.RT-PCR technology has a wide range of applications in RNA virus context of detection.Using made of the technology
Kit is on sale on the market.In biological technical field, kit is to be used to hold detection chemical composition, medicament residue, virus
The box of the chemical reagent such as species, the generation of kit are in order that experimenter can break away from heavy preparation of reagents and optimization
Process.But existing kit, such as RT-PCR diagnostic kits are usually that a placement plate is placed inside kit, its
On be provided with circular hole for placing Reagent Tube, such a kit can only hold one group of RT-PCR reaction reagent, it is impossible to profit well
With the space of kit, kit can not be used for multiple times, and cause to waste, do not utilize environmental protection.
Therefore need it is a kind of simple in construction, it is easy to use, can place and multiple be used to hold multigroup RT-PCR reaction reagents
Plate is placed, and facilitates the superiors to place plate and takes out the RT-PCR quick detection kits that plate ejection is placed with its lower floor, to
Hope solve above-mentioned technical problem.
The problem of the utility model exists according to prior art, there is provided it is a kind of simple in construction, it is easy to use, it can realize
Multiple placement plates are placed to be used to hold multigroup RT-PCR reaction reagents, reused RT-PCR quick detection kits.
Utility model content
One is only set to be used to hold one group of RT- in order to overcome prior art to hold the kit of RT-PCR reaction reagents
The placement plate of PCR reaction reagents, the not reused problem of kit, the utility model provide a kind of reused
RT-PCR quick detection kits.
A kind of reused RT-PCR quick detection kits, including box body, lid, polylith putting with Reagent Tube
Put plate, it is characterised in that the box body is flexibly connected with the side of lid, the restricted screens for placing plate ejection in the box body
Structure and the elastic construction for promoting placement plate to move up, retaining structure is arranged at box body inside side walls top, and retaining structure is located at
The upside of plate is placed in top side, and box body bottom inside is fixed in elastic construction one end, and plate contacts-side-down is placed in one end with lower side；
Place and reagent wells are set on plate, the Reagent Tube for holding reagent is placed sequentially in reagent wells, sets placement plate to take out on box side wall
Portal.
Further, buckle is set on the lid, and the side wall of box body is provided with the projection being engaged with buckle.
Further, there is extraction port lid in the placement plate extraction hole, extraction hole covers setting drawing structure, extracts port lid out
Side is flexibly connected with the side of box body, and remaining position can be set with box body side-anastomosis, retaining structure with placing plate extraction hole
Not on the wall of the same side.
Further, the number for placing plate is 2~8, is vertically arranged successively in box body.
Further, the elastic construction is symmetricly set in box body bottom inside and placed in lower side on the downside of plate, elasticity knot
The number of structure is 1~6.
Further, the convenient groove for extracting placement plate out is being set close to placing for placement plate extraction hole side on plate.Groove
Setting, the convenient plate that will place extracts out.
Further, the retaining structure is the block board being symmetricly set in box body inside side walls, symmetrical block board it
Between distance be less than the size for placing plate, and can guarantee that and reagent wells are completely exposed.The setting of such a structure can will both place plate limit
When making below block board, and not influenceing lid opening, Reagent Tube is taken out.
Further, distance of the retaining structure apart from the most upper edge of box body is more than the thickness of single placement plate.Such a structure
Setting when can both prevent that lid from opening, uppermost placement plate is ejected, and can ensure to place setting for plate extraction hole again
Put.Or distance of the retaining structure apart from the most upper edge of box body is more than the height that most upper test agent pipe spills most upper measuring and puts plate
Degree, and can ensure that Reagent Tube is inelastic to lid more than the thickness of single placement plate, the setting of such a structure, can protect again
Card places the setting of plate extraction hole.
Further, the plate extraction hole of placing is arranged at the box side wall position relative with the placement plate of most upper survey, places
Thickness of the height of plate extraction hole slightly larger than single placement plate.The setting of such a structure, the convenient placement plate by most upper survey is from putting
Put in plate extraction hole and extract out.
Further, the reagent wells placed on plate do not penetrate placement plate.The setting of such a structure can be prevented effectively
Influence of the plate to upside Reagent Tube is placed in downside, and it is possible to prevente effectively from upside uses reagent to downside reagent during use
Pollution.
Further, reagent wells groove is arranged at the reagent wells bottom, and Reagent Tube polycrystalline substance just can intercalating agent hole groove
In, dynamics is moderate, the setting of such a structure, and when can both prevent that placing plate is popped up mobile, Reagent Tube departs from Reagent Tube, again
When can ensure to be extracted Reagent Tube from reagent wells with hand, it is unlikely to too laborious.
Further, the Reagent Tube is the interior Reagent Tube for having RT-PCR reaction reagents.Further, the Reagent Tube is respectively
(1) RT-PCRbuffer is managed, built-in 2 × RT-PCRbuffer；(2) primer pipe, it is built-in to detect the positive anti-primer of virus；(3) enzyme
Pipe, built-in reverse transcriptase and archaeal dna polymerase；(4) deionization water pipe, built-in aseptic deionized water；(5) negative control pipe, it is built-in
Infected biological nucleic acid；(6) positive control pipe, built-in detected viral cDNA positive colonies.
Further, the box body is lucifuge material, wherein it is preferred that black or brown polypropylene material, its light-shading effect is good,
Decomposition of the illumination to nucleic acid can be prevented.
In use, be first turned on that lid exposes most upper survey places plate and Reagent Tube thereon, according to requirement of experiment, successively
The reagent taken out in Reagent Tube is tested, and when the reagent in the Reagent Tube of most upper survey runs out, all Reagent Tubes are extracted,
Then extraction port lid is opened by pulling structure, plate extraction will be placed by placing plate extraction hole, then bullet is put on elastic construction
Plate is put, makes second to place plate and moves up, and is limited by block board, the like, reaching a RT-PCR kit can be multiple
Using effect.
Compared with prior art, the reused RT-PCR quick detection kits of the utility model are easy to use, energy
It is enough to realize that placing multiple placement plates is used to hold multigroup RT-PCR reaction reagents, be used for multiple times.
Brief description of the drawings
Fig. 1 is the utility model overall structure diagram；
Fig. 2 is the utility model extraction hole side structure schematic diagram；
Fig. 3 is the utility model buckle side structure schematic diagram；
Fig. 4, which is that the utility model is right, regards cross-sectional view；
Fig. 5 is to regard cross-sectional view on the utility model；
In figure, 1, box body；11st, retaining structure；12nd, elastic construction；13rd, plate extraction hole is placed；14th, port lid is extracted out；141、
Pull structure；2nd, lid；21st, buckle；22nd, projection；3rd, plate is placed；31st, groove；32nd, reagent wells；33rd, Reagent Tube；331、RT-
PCRbuffer is managed；332nd, primer pipe；333rd, enzyme pipe；334th, deionization water pipe；335th, negative control pipe；336th, positive control pipe.
Embodiment
A kind of reused RT-PCR quick detection kits of embodiment 1
A kind of reused RT-PCR quick detection kits, including box body 1, lid 2, polylith carry Reagent Tube 33
Placement plate 3, it is characterised in that the box body 1 is flexibly connected with the side of lid 2, on lid 2 set buckle 21, box body 1
Side wall is provided with the projection 22 being engaged with buckle 21；The retaining structure 11 and push away that restricted placement plate 3 ejects in the box body 1
The dynamic elastic construction 12 placed plate 3 and moved up, retaining structure 11 is arranged at the inside side walls top of box body 1, and retaining structure 11 is located at
The upside of plate 3 is placed in top side, and the bottom inside of box body 1 is fixed in the one end of elastic construction 12, and the downside of plate 3 is placed in one end with lower side
Contact；Place and reagent wells 32 are set on plate 3, the Reagent Tube 33 for holding reagent is placed sequentially in reagent wells 32, in the side wall of box body 1
Set and place plate extraction hole 13.
Place to have in plate extraction hole 13 and extract port lid 14 out, extract out and drawing structure 141 is set on port lid 14, extract port lid 14 out
Side is flexibly connected with the side of box body 1, and remaining position can be with the side-anastomosis of box body 1, and retaining structure 11 is with placing plate extraction hole
13 are arranged on not on the wall of the same side.
The number for placing plate 3 is 2, is vertically arranged successively in box body 1.
Elastic construction 12 is symmetricly set in the bottom inside of box body 1 and the downside of plate 3, of elastic construction 12 is placed in lower side
Number is 1.
Retaining structure 11 is the distance between the block board, symmetrical block board being symmetricly set in the inside side walls of box body 1
Less than the size of placement plate 3, and it can guarantee that and reagent wells 32 are completely exposed.The setting of such a structure can will both place plate 3 and be limited in
Below block board, but do not influence lid 2 open when, take out Reagent Tube 33.
Distance of the retaining structure 11 apart from the most upper edge of box body 1 is more than the thickness of single placement plate 3.The setting of such a structure was both
When can prevent that lid 2 from opening, uppermost placement plate 3 is ejected, and can ensure to place the setting of plate extraction hole 13 again.
Place plate extraction hole 13 and be arranged at box body 1 sidewall locations relative with the placement plate 3 of most upper survey, place plate extraction hole
Thickness of 13 height slightly larger than single placement plate 3.The setting of such a structure, the convenient placement plate 3 by most upper survey is from placing plate
Extracted out in extraction hole 13.
Place the reagent wells 32 on plate 3 and do not penetrate placement plate 3.The setting of such a structure can effectively prevent downside from placing
Influence of the plate 3 to upside Reagent Tube 33, and it is possible to prevente effectively from upside uses pollution of the reagent to downside reagent during use.
Reagent wells groove arranged at the bottom of reagent wells 32, and the polycrystalline substance of Reagent Tube 33 just can be in the groove of intercalating agent hole, dynamics
Moderate, the setting of such a structure, when can both prevent that placing plate 3 is popped up mobile, Reagent Tube 33 departs from Reagent Tube 33, again may be used
When guarantee is extracted Reagent Tube 33 with hand from reagent wells 32, it is unlikely to too laborious.
Reagent Tube 33 is the interior Reagent Tube 33 for having RT-PCR reaction reagents.Reagent Tube 33 is respectively (1) RT-PCRbuffer
Pipe 331, built-in 2 × RT-PCRbuffer；(2) primer pipe 332, it is built-in to detect the positive anti-primer of virus；(3) enzyme pipe 333, it is built-in
Reverse transcriptase and archaeal dna polymerase；(4) deionization water pipe 334, built-in aseptic deionized water；(5) negative control pipe 335, built-in quilt
The biological nucleic acid of infection；(6) positive control pipe 336, built-in detected viral cDNA positive colonies.
Box body 1 is black polypropylene material, and its light-shading effect is good, can prevent decomposition of the illumination to nucleic acid.
A kind of intoe brace of embodiment 2
A kind of reused RT-PCR quick detection kits, including box body 1, lid 2, polylith carry Reagent Tube 33
Placement plate 3, it is characterised in that the box body 1 is flexibly connected with the side of lid 2, on lid 2 set buckle 21, box body 1
Side wall is provided with the projection 22 being engaged with buckle 21；The retaining structure 11 and push away that restricted placement plate 3 ejects in the box body 1
The dynamic elastic construction 12 placed plate 3 and moved up, retaining structure 11 is arranged at the inside side walls top of box body 1, and retaining structure 11 is located at
The upside of plate 3 is placed in top side, and the bottom inside of box body 1 is fixed in the one end of elastic construction 12, and the downside of plate 3 is placed in one end with lower side
Contact；Place and reagent wells 32 are set on plate 3, the Reagent Tube 33 for holding reagent is placed sequentially in reagent wells 32, in the side wall of box body 1
Set and place plate extraction hole 13.
Place to have in plate extraction hole 13 and extract port lid 14 out, extract out and drawing structure 141 is set on port lid 14, extract port lid 14 out
Side is flexibly connected with the side of box body 1, and remaining position can be with the side-anastomosis of box body 1, and retaining structure 11 is with placing plate extraction hole
13 are arranged on not on the wall of the same side.
The number for placing plate 3 is 4, is vertically arranged successively in box body 1.
Elastic construction 12 is symmetricly set in the bottom inside of box body 1 and the downside of plate 3, of elastic construction 12 is placed in lower side
Number is 2.
The groove 31 for placing plate 3 is conveniently extracted out being set on the placement plate 3 for placing the side of plate extraction hole 13.Groove 31
Setting, the convenient plate 3 that will place extracts out.
Retaining structure 11 is the distance between the block board, symmetrical block board being symmetricly set in the inside side walls of box body 1
Less than the size of placement plate 3, and it can guarantee that and reagent wells 32 are completely exposed.The setting of such a structure can will both place plate 3 and be limited in
Below block board, but do not influence lid 2 open when, take out Reagent Tube 33.
Distance of the retaining structure 11 apart from the most upper edge of box body 1 is more than the thickness of single placement plate 3.The setting of such a structure was both
When can prevent that lid 2 from opening, uppermost placement plate 3 is ejected, and can ensure to place the setting of plate extraction hole 13 again.
Place plate extraction hole 13 and be arranged at box body 1 sidewall locations relative with the placement plate 3 of most upper survey, place plate extraction hole
Thickness of 13 height slightly larger than single placement plate 3.The setting of such a structure, the convenient placement plate 3 by most upper survey is from placing plate
Extracted out in extraction hole 13.
Place the reagent wells 32 on plate 3 and do not penetrate placement plate 3.The setting of such a structure can effectively prevent downside from placing
Influence of the plate 3 to upside Reagent Tube 33, and it is possible to prevente effectively from upside uses pollution of the reagent to downside reagent during use.
Reagent wells groove arranged at the bottom of reagent wells 32, and the polycrystalline substance of Reagent Tube 33 just can be in the groove of intercalating agent hole, dynamics
Moderate, the setting of such a structure, when can both prevent that placing plate 3 is popped up mobile, Reagent Tube 33 departs from Reagent Tube 33, again may be used
When guarantee is extracted Reagent Tube 33 with hand from reagent wells 32, it is unlikely to too laborious.
Reagent Tube 33 is the interior Reagent Tube 33 for having RT-PCR reaction reagents.Reagent Tube 33 is respectively (1) RT-PCRbuffer
Pipe 331, built-in 2 × RT-PCRbuffer；(2) primer pipe 332, it is built-in to detect the positive anti-primer of virus；(3) enzyme pipe 333, it is built-in
Reverse transcriptase and archaeal dna polymerase；(4) deionization water pipe 334, built-in aseptic deionized water；(5) negative control pipe 335, built-in quilt
The biological nucleic acid of infection；(6) positive control pipe 336, built-in detected viral cDNA positive colonies.
Box body 1 is brown polypropylene material, and its light-shading effect is good, can prevent decomposition of the illumination to nucleic acid.
A kind of reused RT-PCR quick detection kits of embodiment 3
A kind of reused RT-PCR quick detection kits, including box body 1, lid 2, polylith carry Reagent Tube 33
Placement plate 3, it is characterised in that the box body 1 is flexibly connected with the side of lid 2, on lid 2 set buckle 21, box body 1
Side wall is provided with the projection 22 being engaged with buckle 21；The retaining structure 11 and push away that restricted placement plate 3 ejects in the box body 1
The dynamic elastic construction 12 placed plate 3 and moved up, retaining structure 11 is arranged at the inside side walls top of box body 1, and retaining structure 11 is located at
The upside of plate 3 is placed in top side, and the bottom inside of box body 1 is fixed in the one end of elastic construction 12, and the downside of plate 3 is placed in one end with lower side
Contact；Place and reagent wells 32 are set on plate 3, the Reagent Tube 33 for holding reagent is placed sequentially in reagent wells 32, in the side wall of box body 1
Set and place plate extraction hole 13.
Place to have in plate extraction hole 13 and extract port lid 14 out, extract out and drawing structure 141 is set on port lid 14, extract port lid 14 out
Side is flexibly connected with the side of box body 1, and remaining position can be with the side-anastomosis of box body 1, and retaining structure 11 is with placing plate extraction hole
13 are arranged on not on the wall of the same side.
The number for placing plate 3 is 8, is vertically arranged successively in box body 1.
Elastic construction 12 is symmetricly set in the bottom inside of box body 1 and the downside of plate 3, of elastic construction 12 is placed in lower side
Number is 6.
The groove 31 for placing plate 3 is conveniently extracted out being set on the placement plate 3 for placing the side of plate extraction hole 13.Groove 31
Setting, the convenient plate 3 that will place extracts out.
Retaining structure 11 is the distance between the block board, symmetrical block board being symmetricly set in the inside side walls of box body 1
Less than the size of placement plate 3, and it can guarantee that and reagent wells 32 are completely exposed.The setting of such a structure can will both place plate 3 and be limited in
Below block board, but do not influence lid 2 open when, take out Reagent Tube 33.
Distance of the retaining structure 11 apart from the most upper edge of box body 1 spills most upper measuring more than most upper test agent pipe 33 and puts plate 3
Highly, and more than the thickness of single placement plate 3, the setting of such a structure it can ensure that Reagent Tube 33 is inelastic to lid 2, again
The setting of placement plate extraction hole 13 can be ensured.
Place plate extraction hole 13 and be arranged at box body 1 sidewall locations relative with the placement plate 3 of most upper survey, place plate extraction hole
Thickness of 13 height slightly larger than single placement plate 3.The setting of such a structure, the convenient placement plate 3 by most upper survey is from placing plate
Extracted out in extraction hole 13.
Place the reagent wells 32 on plate 3 and do not penetrate placement plate 3.The setting of such a structure can effectively prevent downside from placing
Influence of the plate 3 to upside Reagent Tube 33, and it is possible to prevente effectively from upside uses pollution of the reagent to downside reagent during use.
Reagent wells groove arranged at the bottom of reagent wells 32, and the polycrystalline substance of Reagent Tube 33 just can be in the groove of intercalating agent hole, dynamics
Moderate, the setting of such a structure, when can both prevent that placing plate 3 is popped up mobile, Reagent Tube 33 departs from Reagent Tube 33, again may be used
When guarantee is extracted Reagent Tube 33 with hand from reagent wells 32, it is unlikely to too laborious.
Reagent Tube 33 is the interior Reagent Tube 33 for having RT-PCR reaction reagents.Reagent Tube 33 is respectively (1) RT-PCRbuffer
Pipe 331, built-in 2 × RT-PCRbuffer；(2) primer pipe 332, it is built-in to detect the positive anti-primer of virus；(3) enzyme pipe 333, it is built-in
Reverse transcriptase and archaeal dna polymerase；(4) deionization water pipe 334, built-in aseptic deionized water；(5) negative control pipe 335, built-in quilt
The biological nucleic acid of infection；(6) positive control pipe 336, built-in detected viral cDNA positive colonies.
Box body 1 is black polypropylene material, and its light-shading effect is good, can prevent decomposition of the illumination to nucleic acid.
Above example only expresses a kind of embodiment of the present utility model, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the utility model patent scope can not be interpreted as.It should be pointed out that the ordinary skill for this area
For personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these belong to this
The protection domain of utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (10)
1. a kind of reused RT-PCR quick detection kits, including box body (1), lid (2), polylith carry Reagent Tube
(33) placement plate (3), it is characterised in that the box body (1) is flexibly connected with the side of lid (2), has in the box body (1)
Limitation, which is placed the retaining structure (11) of plate (3) ejection and promoted, places the elastic construction (12) that plate (3) moves up, retaining structure (11)
Box body (1) inside side walls top is arranged at, and retaining structure (11) is located at the upside that plate (3) is placed in top side, elastic construction
(12) box body (1) bottom inside is fixed in one end, and plate (3) contacts-side-down is placed in one end with lower side；Place and examination is set on plate (3)
Agent hole (32), the Reagent Tube (33) for holding reagent are placed sequentially in reagent wells (32), set placement plate to take out in box body (1) side wall
Portal (13).
2. reused RT-PCR quick detection kits according to claim 1, it is characterised in that the placement
There is extraction port lid (14) in plate extraction hole (13), extract out and drawing structure (141) is set on port lid (14), extract the one of port lid (14) out
Side is flexibly connected with the side of box body (1), and remaining position can take out with box body (1) side-anastomosis, retaining structure (11) with placing plate
Portal (13) be arranged on not on the wall of the same side.
3. reused RT-PCR quick detection kits according to claim 1, it is characterised in that the placement
The number of plate (3) is 2~8, is vertically arranged successively in box body (1).
4. reused RT-PCR quick detection kits according to claim 1, it is characterised in that the elasticity
Structure (12) is symmetricly set in box body (1) bottom inside and placed in lower side on the downside of plate (3), and the number of elastic construction (12) is
1~6.
5. reused RT-PCR quick detection kits according to claim 1, it is characterised in that put close
Put and the convenient groove (31) extracted out and place plate (3) is set on the placement plate (3) of plate extraction hole (13) side.
6. reused RT-PCR quick detection kits according to claim 1, it is characterised in that the screens
Structure (11) is that the distance between the block board, symmetrical block board being symmetricly set in box body (1) inside side walls is less than placement
The size of plate (3), and can guarantee that and reagent wells (32) are completely exposed.
A 7. reused RT-PCR quick detection kit according to claim 6, it is characterised in that the card
Distance of the bit architecture (11) apart from the most upper edge of box body (1) is more than the thickness of single placement plate (3).
A 8. reused RT-PCR quick detection kit according to claim 1, it is characterised in that described to put
Plate extraction hole (13) is put to be arranged at and relative box body (1) sidewall locations of the placement plate of most upper survey (3), placement plate extraction hole (13)
Height slightly larger than it is single placement plate (3) thickness.
A 9. reused RT-PCR quick detection kit according to claim 1, it is characterised in that described to put
Put the reagent wells (32) on plate (3) and do not penetrate placement plate (3).
A 10. reused RT-PCR quick detection kit according to claim 1, it is characterised in that described
Reagent Tube (33) is the interior Reagent Tube (33) for having RT-PCR reaction reagents.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721061246.5U CN207130266U (en) | 2017-08-23 | 2017-08-23 | A kind of reused RT PCR quick detection kits |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721061246.5U CN207130266U (en) | 2017-08-23 | 2017-08-23 | A kind of reused RT PCR quick detection kits |
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| CN207130266U true CN207130266U (en) | 2018-03-23 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109971642A (en) * | 2019-04-30 | 2019-07-05 | 江苏三和生物工程股份有限公司 | A kind of medical instrument for pathogen quick diagnosis |
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2017
- 2017-08-23 CN CN201721061246.5U patent/CN207130266U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109971642A (en) * | 2019-04-30 | 2019-07-05 | 江苏三和生物工程股份有限公司 | A kind of medical instrument for pathogen quick diagnosis |
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