CN204607990U - The test kit that a kind of RET fusion gene detects - Google Patents
The test kit that a kind of RET fusion gene detects Download PDFInfo
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- CN204607990U CN204607990U CN201520302950.XU CN201520302950U CN204607990U CN 204607990 U CN204607990 U CN 204607990U CN 201520302950 U CN201520302950 U CN 201520302950U CN 204607990 U CN204607990 U CN 204607990U
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Abstract
The utility model discloses the test kit that a kind of RET fusion gene detects, comprise test kit body, main lid, Moveable lid body, fixing inner box, sponge cushion, the first sealing paste, the second sealing paste, branched EP pipe, sealed strip and top protective cover; Main lid is connected with test kit body by sealed strip; Main lid is connected with Moveable lid body, and Moveable lid body cover cap is in test kit body front end; First sealing paste is located on Moveable lid body, and the second sealing paste is located on test kit body, and the first sealing paste is connected with the second sealing paste adaptation; Fixing inner box is located in test kit body, and sponge cushion is located in fixing inner box, and EP pipe is inserted in sponge cushion; Top protective cover is located in main lid, and top protective cover cover cap is on EP pipe.The utility model has when RET gene fusion detects that susceptibility is high, high specificity, the advantage such as short consuming time, and the design of container hole makes container bottom to collide with breakage, guaranteed reagent,G.R. box sealingly secure, convenient transport.
Description
Technical field
The utility model relates to a kind of detection kit technical field, particularly relates to the test kit that a kind of RET fusion gene detects.
Background technology
In prior art, detect the multiple RT-PCR detection kit of a kind of RET transgenation fusion used, general structure comprises box body, box body top is provided with lid, permanent seat and reagent bottle groove is provided with in the cavity of box body, permanent seat is provided with 1.5ml EP tubular container hole and 0.5ml EP tubular container hole, be provided with 1.5ml EP in described 1.5ml EP tubular container hole to manage, be provided with 0.5ml EP in described 0.5ml EP tubular container hole and manage, in described reagent bottle groove, be provided with dress PCR buffer solution mixture reagent bottle.
Utility model content
The purpose of this utility model: the test kit that a kind of RET fusion gene detects is provided, structure is simple, can prevent the container bottom in test kit to collide breakage, ensure the stopping property of lid and box body, convenient mobile and transport, experimenter can being facilitated to operate when detecting to RET gene fusion, increasing work efficiency.
To achieve these goals, the technical solution of the utility model is:
The test kit that RET fusion gene detects, comprises test kit body, main lid, Moveable lid body, fixing inner box, sponge cushion, the first sealing paste, the second sealing paste, branched EP pipe, sealed strip and top protective cover; One end of described main lid is hinged on the top of described test kit body by articulated elements, described main lid cover cap is on described test kit body, described sealed strip is arranged on the lower surface of described main lid, and described main lid is tightly connected by described sealed strip and described test kit body; The other end of described main lid is connected with one end of described Moveable lid body, and described main lid and described Moveable lid body are structure as a whole, and described Moveable lid body cover cap is on the front end face of described test kit body; The first described sealing paste is fixedly installed on described Moveable lid body, and the second described sealing paste is fixedly installed on the front end face of described test kit body, and the first described sealing paste is connected with the second described sealing paste adaptation; Described fixing inner box is fixedly installed in described test kit body, and described fixing inner box and described test kit body link into an integrated entity structure; Described sponge cushion is arranged in described fixing inner box, and be provided with multiple slot in described sponge cushion, the lower end of described branched EP pipe is corresponding to be respectively inserted in the slot of described sponge cushion, the upper surface of described sponge cushion is positioned at the middle and upper part of described branched EP pipe, and the capacity of described branched EP pipe is 0.5ml and 1.5ml; Described top protective cover is arranged in described main lid, and described top protective cover cover cap is in the upper end of described branched EP pipe.
The test kit that above-mentioned RET fusion gene detects, wherein, described top protective cover is interval with multiple top groove, and the upper end correspondence of described branched EP pipe is inserted in described top groove.
The test kit that above-mentioned RET fusion gene detects, wherein, is equipped with the solution detecting different fused type respectively in described branched 1.5ml EP pipe, comprises:
KIF5B-15,16,22,23, CCD1, CCD2, ERC11, GOLGA7F, NCOA8F, HOOK11F, PCM29F, PRKAR7F, KTN29F and RET-12 gene fusion detection reaction mixed solution MIX1;
KIF5B-24 and RET-11-1 gene fusion detection reaction mixed solution MIX2;
KIF5B-15 and RET-11-2 gene fusion detection reaction mixed solution MIX3;
Actin gene test is with reference to mixed solution MIX4;
Described liquor capacity is 210ul.
The test kit that above-mentioned RET fusion gene detects, wherein, is equipped with general ddH in described branched 0.5ml EP pipe respectively
2o, reverse transcription primer and positive control plasmid and warm start enzyme.
The utility model structure is simple, reasonable in design, easy to use, easy to manufacture, testing process not vulnerable to pollution, test kit has when RET gene fusion detects that susceptibility is high, high specificity, the advantage such as short consuming time, the design of container hole makes container bottom to collide with breakage, guaranteed reagent,G.R. box sealingly secure, convenient transport.
Accompanying drawing explanation
Fig. 1 is the structural representation of the test kit that a kind of RET fusion gene of the utility model detects.
Embodiment
Embodiment of the present utility model is further illustrated below in conjunction with accompanying drawing.
Refer to shown in accompanying drawing 1, the test kit that RET fusion gene detects, comprises test kit body 1, main lid 2, Moveable lid body 3, fixing inner box 4, sponge cushion 5, first sealing paste 6, second sealing paste 7, branched EP pipe 8, sealed strip 9 and top protective cover 10; One end of described main lid 2 is hinged on the top of described test kit body 1 by articulated elements, described main lid 2 cover cap is on described test kit body 1, described sealed strip 9 is arranged on the lower surface of described main lid 2, and described main lid 2 is tightly connected with described test kit body 1 by described sealed strip 9; The other end of described main lid 2 is connected with one end of described Moveable lid body 3, and described main lid 2 is structure as a whole with described Moveable lid body 3, and described Moveable lid body 3 cover cap is on the front end face of described test kit body 1; The first described sealing paste 6 is fixedly installed on described Moveable lid body 3, and the second described sealing paste 7 is fixedly installed on the front end face of described test kit body 1, and the first described sealing paste 6 is connected with the second described sealing paste 7 adaptation; Described fixing inner box 4 is fixedly installed in described test kit body 1, and described fixing inner box 4 and described test kit body 1 link into an integrated entity structure; Described sponge cushion 5 is arranged in described fixing inner box 4, and be provided with multiple slot 11 in described sponge cushion 5, the lower end of described branched EP pipe 8 is corresponding to be respectively inserted in the slot 11 of described sponge cushion 5, the upper surface of described sponge cushion 5 is positioned at the middle and upper part of described branched EP pipe 8, and the capacity of described branched EP pipe 8 is 0.5ml and 1.5ml; Described top protective cover 10 is arranged in described main lid 2, and described top protective cover 10 cover cap is in the upper end of described branched EP pipe 8.
Described top protective cover 10 is interval with multiple top groove 12, and the upper end correspondence of described branched EP pipe 8 is inserted in described top groove 12, can be used for the upper end protecting EP pipe 8.
In described branched 1.5ml EP pipe 8, the solution detecting different fused type is housed respectively, comprises:
KIF5B-15,16,22,23, CCD1, CCD2, ERC11, GOLGA7F, NCOA8F, HOOK11F, PCM29F, PRKAR7F, KTN29F and RET-12 gene fusion detection reaction mixed solution MIX1;
KIF5B-24 and RET-11-1 gene fusion detection reaction mixed solution MIX2;
KIF5B-15 and RET-11-2 gene fusion detection reaction mixed solution MIX3;
Actin gene test is with reference to mixed solution MIX4;
Described liquor capacity is 210ul.
In described branched 0.5ml EP pipe 8, general ddH is housed respectively
2o, reverse transcription primer and positive control plasmid and warm start enzyme.
Working method of the present utility model:
1) template DNA is got
Get patients blood 1mL, traditional extraction DNA, get 1uLDNA as template.
2) reaction system
Each reactive material is carried out proportioning according to following middle reaction system;
Reaction system is 25ul, comprises qPCR mixed solution 20ul, with reference to primer or ARMS primer each 0.5ul(final concentration 0.5 uM/L), template 1ul(100ng),
Template 1ul
With reference to primer or ARMS primer final concentration 0.5 uM/L
PCR buffer Final concentration 1X
DNTPs final concentration is 0.2mM
General downstream primer final concentration is 0.5uM
TagMan probe final concentration is 0.1uM
Goldstar Best Tag enzyme final concentration is 0.05U/ul
MgCl2 final concentration is 2mM
3) system reaction
Merge multiple RT-PCR completely on ABI 7500, to detect each sample carry out 3 tube reactions, often pipe adds identical qPCR mix, general downstream primer, TaqMan probe, and each pipe difference is with reference to primer or ARMS primer.QPCR reaction conditions is: 95 ° of C denaturation 10min, 95 ° of C 15s, 60 ° of C 1min, and amplified reaction is 46 circulations.
In sum, the utility model structure is simple, reasonable in design, easy to use, easy to manufacture, testing process not vulnerable to pollution, test kit has when RET gene fusion detects that susceptibility is high, high specificity, the advantage such as short consuming time, the design of container hole makes container bottom to collide with breakage, guaranteed reagent,G.R. box sealingly secure, convenient transport.
The foregoing is only preferred embodiment of the present utility model; not thereby the scope of the claims of the present utility model is limited; every equivalent structure transformation utilizing the utility model description to do; or directly or indirectly use the technical field being attached to other related productss, be all in like manner included in scope of patent protection of the present utility model.
Claims (4)
1. a test kit for RET fusion gene detection, is characterized in that: comprise test kit body, main lid, Moveable lid body, fixing inner box, sponge cushion, the first sealing paste, the second sealing paste, branched EP pipe, sealed strip and top protective cover; One end of described main lid is hinged on the top of described test kit body by articulated elements, described main lid cover cap is on described test kit body, described sealed strip is arranged on the lower surface of described main lid, and described main lid is tightly connected by described sealed strip and described test kit body; The other end of described main lid is connected with one end of described Moveable lid body, and described main lid and described Moveable lid body are structure as a whole, and described Moveable lid body cover cap is on the front end face of described test kit body; The first described sealing paste is fixedly installed on described Moveable lid body, and the second described sealing paste is fixedly installed on the front end face of described test kit body, and the first described sealing paste is connected with the second described sealing paste adaptation; Described fixing inner box is fixedly installed in described test kit body, and described fixing inner box and described test kit body link into an integrated entity structure; Described sponge cushion is arranged in described fixing inner box, and be provided with multiple slot in described sponge cushion, the lower end of described branched EP pipe is corresponding to be respectively inserted in the slot of described sponge cushion, the upper surface of described sponge cushion is positioned at the middle and upper part of described branched EP pipe, and the capacity of described branched EP pipe is 0.5ml and 1.5ml; Described top protective cover is arranged in described main lid, and described top protective cover cover cap is in the upper end of described branched EP pipe.
2. the test kit of RET fusion gene detection according to claim 1, is characterized in that: described top protective cover is interval with multiple top groove, and the upper end correspondence of described branched EP pipe is inserted in described top groove.
3. the test kit of RET fusion gene detection according to claim 1, is characterized in that: in described branched 1.5ml EP pipe, the solution detecting different fused type is housed respectively, comprises:
KIF5B-15,16,22,23, CCD1, CCD2, ERC11, GOLGA7F, NCOA8F, HOOK11F, PCM29F, PRKAR7F, KTN29F and RET-12 gene fusion detection reaction mixed solution MIX1;
KIF5B-24 and RET-11-1 gene fusion detection reaction mixed solution MIX2;
KIF5B-15 and RET-11-2 gene fusion detection reaction mixed solution MIX3;
Actin gene test is with reference to mixed solution MIX4;
Described liquor capacity is 210ul.
4. the test kit of RET fusion gene detection according to claim 1, is characterized in that: in described branched 0.5ml EP pipe, general ddH is housed respectively
2o, reverse transcription primer and positive control plasmid and warm start enzyme.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520302950.XU CN204607990U (en) | 2015-05-12 | 2015-05-12 | The test kit that a kind of RET fusion gene detects |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520302950.XU CN204607990U (en) | 2015-05-12 | 2015-05-12 | The test kit that a kind of RET fusion gene detects |
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| Publication Number | Publication Date |
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| CN204607990U true CN204607990U (en) | 2015-09-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520302950.XU Active CN204607990U (en) | 2015-05-12 | 2015-05-12 | The test kit that a kind of RET fusion gene detects |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635775A (en) * | 2016-12-26 | 2017-05-10 | 苏州欣祥本机械科技有限公司 | Covered hermetic molecular diagnosis kit |
| CN106754307A (en) * | 2016-12-07 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of kit of RET fusions detection |
-
2015
- 2015-05-12 CN CN201520302950.XU patent/CN204607990U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754307A (en) * | 2016-12-07 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of kit of RET fusions detection |
| CN106635775A (en) * | 2016-12-26 | 2017-05-10 | 苏州欣祥本机械科技有限公司 | Covered hermetic molecular diagnosis kit |
| CN106635775B (en) * | 2016-12-26 | 2019-02-22 | 苏州欣祥本机械科技有限公司 | A kind of sealing molecule diagnosis kit of cover-type |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 314001 building 5-1, 3556 linggongtang Road, Daqiao Town, Nanhu District, Jiaxing City, Zhejiang Province Patentee after: Zhejiang Yunying Medical Technology Co.,Ltd. Address before: 201416 room 355, building 13, No. 1111, Xinlin Road, Fengxian District, Shanghai Patentee before: SHANGHAI YUNYING MEDICAL TECHNOLOGY Co.,Ltd. |
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| CP03 | Change of name, title or address |