CN216550413U - Tube assembly for one-tube reagent freeze-drying and PCR reaction - Google Patents
Tube assembly for one-tube reagent freeze-drying and PCR reaction Download PDFInfo
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- CN216550413U CN216550413U CN202122568976.7U CN202122568976U CN216550413U CN 216550413 U CN216550413 U CN 216550413U CN 202122568976 U CN202122568976 U CN 202122568976U CN 216550413 U CN216550413 U CN 216550413U
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Abstract
The utility model provides a tube assembly for carrying out reagent freeze-drying and PCR reaction in a tube type, wherein a first tube plug of a PCR cover is vertically inserted into the cylindrical upper part of a tube body, the outer side surface of the first tube plug abuts against the inner wall of the cylindrical upper part, a first cover plate is horizontally arranged on the first tube plug and abuts against the top end of the cylindrical upper part and an annular flange, and a clamping hook is vertically arranged, positioned below the first cover plate and hooked on the bottom surface of the annular flange; the second stopcock of freeze-drying stopper is used for vertical the inserting to be established in cylindrical upper portion, and the lateral surface is used for leaning on the inner wall on cylindrical upper portion, and the second apron level sets up on the second stopcock and is used for leaning on top and the annular flange on cylindrical upper portion. The utility model can realize one-tube operation from reagent freeze-drying to PCR reaction, can be used for reagent freeze-drying, meets the freeze-drying requirement, is suitable for long-distance transportation, can be used for PCR reaction, reduces reagent loss and material consumption cost in the detection process, prevents liquid overflow and gas volatilization, and avoids pollution.
Description
Technical Field
The utility model relates to the technical field of biochemical experiment consumables, in particular to the technical field of reagent freeze-drying and PCR reaction pipe fittings, and specifically relates to a pipe assembly for carrying out reagent freeze-drying and PCR reaction in a pipe type.
Background
PCR (Polymerase Chain Reaction) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and can be regarded as special DNA replication in vitro, and the biggest characteristic of PCR is that a trace amount of DNA can be greatly increased.
PCR has been widely used due to its advantages of high sensitivity, good specificity, convenient operation, rapid reaction, etc., but it is very sensitive, and trace contamination sources may cause false positive of the detection result, so how to avoid laboratory contamination is especially important for PCR experiments.
The PCR experiment is gone on usually in the PCR pipe, current PCR pipe generally includes body and tube cap, the vertical setting of body, divide into upper portion and lower part, upper portion sets up on the lower part, upper portion is cylindrical, the lower part is the obconic shape, the lateral surface level on upper portion top is provided with the annular flange, the tube cap includes stopcock and apron, the apron level sets up and supports on top and the annular flange that leans on the upper portion of body, the stopcock is vertical to be inserted and to be established in the upper portion of body and lie in under the apron and connect the apron, the inner wall on the upper portion of the lateral surface laminating body of stopcock, the apron passes through connecting strip and connects the annular flange, PCR pipe integrated into one piece usually. However, the existing PCR tube has poor sealing performance of the tube cover and the tube body, the leakage rate of the liquid in the tube body is only ensured to be not higher than 3% at high temperature, and the gas in the tube body is easy to overflow to cause aerosol pollution; in addition, the tube cover risks contamination due to incorrect opening.
In addition, the PCR reaction reagent is usually lyophilized in a lyophilization tube or vial, and when performing the PCR reaction, the PCR reaction reagent in the lyophilization tube or vial needs to be thawed and then transferred into the PCR tube, which causes reagent loss.
Therefore, it is desirable to provide a tube assembly for performing reagent lyophilization and PCR reaction in one tube type, which can use the same tube body from reagent lyophilization to PCR reaction without loss due to transfer, while ensuring stability of lyophilized reagents, facilitating long-distance transportation, and direct use in PCR reaction, preventing liquid overflow and gas volatilization, and avoiding contamination.
SUMMERY OF THE UTILITY MODEL
In order to overcome the disadvantages in the prior art, an object of the present invention is to provide a tube assembly for performing reagent lyophilization and PCR reaction in a tube type, which can perform one-tube operation from reagent lyophilization to PCR reaction, and can be used for reagent lyophilization preparation, satisfying lyophilization requirements, being suitable for long-distance transportation, being directly used for PCR reaction, reducing reagent loss and consumable material cost in a detection process, preventing liquid overflow and gas volatilization in a PCR reaction process, avoiding pollution, and being suitable for large-scale popularization and application.
Another object of the present invention is to provide a tube assembly for performing reagent lyophilization and PCR reaction in a single tube type, which has smart design, simple structure, easy manufacture, low cost, and is suitable for large-scale popularization and application.
In order to achieve the above objects, the present invention provides a tube assembly for performing reagent lyophilization and PCR reaction in one tube type, comprising a tube body vertically disposed, the tube body including a cylindrical upper portion, an inverted conical lower portion, and an annular flange, the cylindrical upper portion being disposed on the inverted conical lower portion, the annular flange being horizontally sleeved on an outer side surface of a top end of the cylindrical upper portion, wherein the tube assembly for performing reagent lyophilization and PCR reaction in one tube type further comprises a PCR cap and a lyophilization plug, wherein:
the PCR cover comprises a first cover plate, a first pipe plug and a clamping hook, wherein the first pipe plug is vertically arranged, the first cover plate is horizontally arranged on the first pipe plug, the clamping hook is vertically arranged and positioned below the first cover plate, the upper end of the clamping hook is connected with the first cover plate, the lower end of the clamping hook is a hook part, the hook part is arranged towards the first pipe plug, the first pipe plug is vertically inserted into the cylindrical upper part, the outer side surface of the first pipe plug abuts against the inner wall of the cylindrical upper part, the first cover plate abuts against the top end of the cylindrical upper part and the annular flange, the middle part of the clamping hook is positioned outside the annular flange, and the hook part is hooked on the bottom surface of the annular flange;
the freeze-drying plug comprises a second cover plate and a second pipe plug, the second pipe plug is vertically arranged, the second cover plate is horizontally arranged on the second pipe plug, the second pipe plug is used for being vertically inserted into the cylindrical upper portion, the outer side face of the second pipe plug is used for abutting against the inner wall of the cylindrical upper portion, and the second cover plate is used for abutting against the top end of the cylindrical upper portion and the annular flange.
Preferably, the PCR cap further comprises a first sealing ring, and the first sealing ring is horizontally sleeved on the outer side surface of the lower end of the first plug and abuts against the inner wall of the upper cylindrical part.
Preferably, a first annular groove is formed in the outer side surface of the lower end of the first pipe plug along the circumferential direction of the first pipe plug, and the first sealing ring is horizontally embedded in the first annular groove.
Preferably, the number of the hooks is multiple, and the hooks are arranged around the first pipe plug at intervals.
Preferably, the PCR cap further comprises a second sealing ring horizontally sleeved on the outer side surface of the upper end of the first plug and abutting against the inner wall of the cylindrical upper portion and lower than the annular flange.
Preferably, a second annular groove is formed in the outer side face of the upper end of the first pipe plug along the circumferential direction of the first pipe plug, and the second sealing ring is horizontally embedded in the second annular groove.
Preferably, the PCR cover further comprises a grip part disposed on the first cover plate.
Preferably, the outer side surface of the lower part of the second pipe plug is provided with a vertical groove, the lower end of the vertical groove is an opening, and the opening is arranged on the bottom surface of the second pipe plug.
Preferably, the outer side surface of the lower part of the second pipe plug is provided with a first horizontal annular protrusion, the vertical groove is arranged in the first horizontal annular protrusion, and the first horizontal annular protrusion is used for abutting against the inner wall of the cylindrical upper part.
Preferably, the outer side of the upper portion of the second plug has a second horizontal annular projection for abutting against the inner wall of the cylindrical upper portion.
The utility model has the following beneficial effects:
1. the first pipe plug of the PCR cover of the pipe assembly for carrying out reagent freeze-drying and PCR reaction in a tubular mode is vertically arranged and vertically inserted in the cylindrical upper portion of the pipe body, the outer side face of the first pipe plug abuts against the inner wall of the cylindrical upper portion, the first cover plate is horizontally arranged on the first pipe plug and abuts against the top end of the cylindrical upper portion and the annular flange, the clamping hook is vertically arranged and located below the first cover plate, the upper end of the clamping hook is connected with the first cover plate, the middle portion of the clamping hook is located outside the annular flange, the lower end of the clamping hook is a hook portion, the clamping hook faces the first pipe plug, and the clamping hook is arranged on the bottom face of the annular flange; vertical setting of second stopcock of freeze-drying stopper is used for vertical inserting to establish in cylindrical upper portion, the lateral surface is used for leaning on the inner wall on cylindrical upper portion, second apron level sets up on the second stopcock and is used for leaning on top and the annular flange on cylindrical upper portion, therefore, it can realize from reagent freeze-drying to a PCR reaction tubular operation, can enough be used for reagent freeze-drying, satisfy freeze-drying requirement, be suitable for long-distance transport, can directly be used for PCR reaction again, reduce the reagent loss and reduce the consumptive material cost among the testing process, prevent that liquid from spilling over and gas volatilizees among the PCR reaction process, avoid polluting, be suitable for extensive popularization and application.
2. The first pipe plug of the PCR cover of the pipe assembly for carrying out reagent freeze-drying and PCR reaction in a tubular mode is vertically arranged and vertically inserted in the cylindrical upper portion of the pipe body, the outer side face of the first pipe plug abuts against the inner wall of the cylindrical upper portion, the first cover plate is horizontally arranged on the first pipe plug and abuts against the top end of the cylindrical upper portion and the annular flange, the clamping hook is vertically arranged and located below the first cover plate, the upper end of the clamping hook is connected with the first cover plate, the middle portion of the clamping hook is located outside the annular flange, the lower end of the clamping hook is a hook portion, the clamping hook faces the first pipe plug, and the clamping hook is arranged on the bottom face of the annular flange; the second stopcock of freeze-drying stopper is vertical to be set up and is used for vertically inserting and establish in cylindrical upper portion, and the lateral surface is used for leaning on the inner wall on cylindrical upper portion, and the second apron level sets up on the second stopcock and is used for leaning on top and the annular flange on cylindrical upper portion, consequently, its design benefit, the structure is succinct, makes portably, and is with low costs, is suitable for extensive popularization and application.
These and other objects, features and advantages of the present invention will become more fully apparent from the following detailed description and accompanying drawings, wherein like reference numerals refer to like parts throughout the several views, and wherein like reference numerals refer to like parts throughout.
Drawings
FIG. 1 is a schematic front view of an embodiment of the tube assembly for one-tube reagent lyophilization and PCR reactions of the present invention.
FIG. 2 is a perspective view of the PCR cap of FIG. 1 covering the tube.
FIG. 3 is a perspective view of the PCR tube of the embodiment shown in FIG. 1.
FIG. 4 is a perspective view of the PCR cap of the embodiment shown in FIG. 1.
Fig. 5 is a perspective view of the freeze-dried plug of the embodiment shown in fig. 1.
Fig. 6 is a perspective view of the embodiment of fig. 1 showing the lyophilization stopper cover disposed on the tube body.
(symbol description)
1, a pipe body; 2, PCR covering; 3, freeze-drying the plug; 4 a cylindrical upper portion; 5 an inverted conical lower part; 6 an annular flange; 7 a first cover plate; 8 a first plug; 9 a first seal ring; 10 hooks; 11 a hook part; 12 a second cover plate; 13 a second plug; 14 vertical grooves; 15 is provided with an opening; 16 a first annular groove; 17 a second seal ring; 18 a second annular groove; 19 a grasping part; 20 a first horizontal annular projection; 21 second horizontal annular projection.
Detailed Description
In order to clearly understand the technical contents of the present invention, the following examples are given in detail.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1 to 6, in an embodiment of the present invention, a tube assembly for reagent lyophilization and PCR reaction in one tube type according to the present invention includes a tube body 1, a PCR cap 2, and a lyophilization plug 3, wherein:
the pipe body 1 is vertically arranged, the pipe body 1 comprises a cylindrical upper part 4, an inverted conical lower part 5 and an annular flange 6, the cylindrical upper part 4 is arranged on the inverted conical lower part 5, and the annular flange 6 is horizontally sleeved on the outer side surface of the top end of the cylindrical upper part 4;
the PCR cover 2 comprises a first cover plate 7, a first pipe plug 8 and a hook 10, wherein the first pipe plug 8 is vertically arranged, the first cover plate 7 is horizontally arranged on the first pipe plug 8, the hook 10 is vertically arranged and positioned below the first cover plate 7, the upper end of the hook 10 is connected with the first cover plate 7, the lower end of the hook 10 is a hook part 11, the hook part 11 is arranged towards the first pipe plug 8, the first pipe plug 8 is vertically inserted in the cylindrical upper part 4, the outer side surface of the first pipe plug 8 abuts against the inner wall of the cylindrical upper part 4, the first cover plate 7 abuts against the top end of the cylindrical upper part 4 and the annular flange 6, the middle part of the hook 10 is positioned outside the annular flange 6, and the hook part 11 is hooked on the bottom surface of the annular flange 6;
the freeze-drying plug 3 comprises a second cover plate 12 and a second pipe plug 13, the second pipe plug 13 is vertically arranged, the second cover plate 12 is horizontally arranged on the second pipe plug 13, the second pipe plug 13 is used for being vertically inserted into the cylindrical upper portion 4, the outer side surface of the second pipe plug 13 is used for abutting against the inner wall of the cylindrical upper portion 4, and the second cover plate 12 is used for abutting against the top end of the cylindrical upper portion 4 and the annular flange 6.
The outer side surface of the first plug 8 abuts against the inner wall of the cylindrical upper portion 4, and any suitable structure can be adopted, as shown in fig. 4, in an embodiment of the present invention, the PCR cap further includes a first sealing ring 9, and the first sealing ring 9 horizontally sleeves the outer side surface of the lower end of the first plug 8 and abuts against the inner wall of the cylindrical upper portion 4. With the arrangement, the first sealing ring 9 can further improve the sealing property between the PCR cover 2 and the tube body 1.
The first sealing ring 9 is horizontally sleeved on the outer side surface of the lower end of the first pipe plug 8, and any suitable structure can be adopted, as shown in fig. 4, in an embodiment of the present invention, a first annular groove 16 is arranged on the outer side surface of the lower end of the first pipe plug 8 along the circumferential direction of the first pipe plug 8, and the first sealing ring 9 is horizontally embedded in the first annular groove 16.
The number of the first sealing rings 9 can be determined as required, preferably, the number of the first sealing rings 9 is multiple, and the first sealing rings 9 are vertically arranged at intervals. Referring to fig. 4, in an embodiment of the present invention, the number of the first sealing rings 9 is 2.
The number of the hooks 10 can be determined according to the requirement, and preferably, the number of the hooks 10 is plural, and the hooks 10 are arranged around the first plug 8 at intervals. Referring to fig. 1 to 2 and fig. 4, in an embodiment of the present invention, the number of the hooks 10 is 2, and 2 hooks 10 are symmetrically arranged.
The outer side of the first plug 8 abuts against the inner wall of the cylindrical upper portion 4, and any suitable structure can be adopted, as shown in fig. 4, in an embodiment of the present invention, the PCR cap 2 further includes a second sealing ring 17, and the second sealing ring 17 horizontally sleeves the outer side of the upper end of the first plug 8 and abuts against the inner wall of the cylindrical upper portion 4 and is lower than the annular flange 6. With the arrangement, the second sealing ring 17 can further improve the sealing performance between the PCR cap 2 and the tube body 1.
The second sealing ring 17 is horizontally sleeved on the outer side surface of the upper end of the first pipe plug 8, and any suitable structure can be adopted, as shown in fig. 4, in an embodiment of the present invention, a second annular groove 18 is arranged on the outer side surface of the upper end of the first pipe plug 8 along the circumferential direction of the first pipe plug 8, and the second sealing ring 17 is horizontally embedded in the second annular groove 18.
The PCR cap 2 can also include any other suitable structure, as shown in fig. 1-2 and 4, in an embodiment of the present invention, the PCR cap 2 further includes a handle 19, and the handle 19 is disposed on the first cover plate 7. With the adoption of the arrangement, the grabbing and pinching part 19 is convenient for an experimenter to grab and pinch, and is convenient to operate.
The grip portion 19 may have any suitable shape, as shown in fig. 1-2 and 4, and in one embodiment of the present invention, the grip portion 19 is in the shape of an inverted truncated cone.
In order to facilitate the outflow of gas in the tube body 1 during reagent freeze-drying, referring to fig. 1 and 5, in an embodiment of the present invention, a vertical groove 14 is disposed on an outer side surface of a lower portion of the second tube plug 13, a lower end of the vertical groove 14 is an opening 15, and the opening 15 is disposed on a bottom surface of the second tube plug 13.
The number of the vertical grooves 14 can be determined as required, and preferably, the number of the vertical grooves 14 is plural, and the plural vertical grooves 14 are arranged at intervals along the circumferential direction of the second pipe plug 13. Referring to fig. 5, in an embodiment of the present invention, the number of the vertical grooves 14 is 4.
The outer side of the second pipe plug 13 is used to abut against the inner wall of the cylindrical upper portion 4, and any suitable structure can be adopted, as shown in fig. 1 and 5, in an embodiment of the present invention, the outer side of the lower portion of the second pipe plug 13 has a first horizontal annular protrusion 20, the vertical groove 14 is disposed in the first horizontal annular protrusion 20, and the first horizontal annular protrusion 20 is used to abut against the inner wall of the cylindrical upper portion 4. With the above arrangement, the first horizontal annular protrusion 20 can further improve the sealing performance between the lyophilization plug 3 and the tube body 1.
The first horizontal annular protrusion 20 may have any suitable shape, as shown in fig. 1 and 5, and in one embodiment of the present invention, the first horizontal annular protrusion 20 is an arc-shaped protrusion.
The outer side of the second pipe plug 13 is used to abut against the inner wall of the cylindrical upper portion 4, and any suitable structure can be adopted, as shown in fig. 1 and 5, in an embodiment of the present invention, the outer side of the upper portion of the second pipe plug 13 is provided with a second horizontal annular protrusion 21, and the second horizontal annular protrusion 21 is used to abut against the inner wall of the cylindrical upper portion 4. With the above arrangement, the second horizontal annular protrusion 21 can further improve the sealing property between the lyophilization plug 3 and the tube body 1.
The second horizontal annular protrusion 21 may have any suitable shape, as shown in fig. 1 and 5, and in an embodiment of the present invention, the second horizontal annular protrusion 21 is an arc-shaped protrusion.
The PCR cap 2 may be a PCR cap of any suitable material, and in one embodiment of the present invention, the PCR cap 2 is a plastic cap.
The freeze-drying plug 3 may be a freeze-drying plug made of any suitable material, and in an embodiment of the present invention, the freeze-drying plug 3 is a silica gel plug.
When the freeze-drying device is used, when reagent freeze-drying is carried out, the reagent is added into the tube body 1, then the freeze-drying plug 3 is covered on the tube body 1, specifically, the lower part of the second tube plug 13 of the freeze-drying plug 3 is vertically inserted into the cylindrical upper part 4 of the tube body 1, the first horizontal annular bulge 20 is abutted against the inner wall of the cylindrical upper part 4 to play a role in fixing the freeze-drying plug 3 and the tube body 1 and ensure that the gas inside flows out through the vertical groove 14, then freeze-drying is carried out, after the freeze-drying is finished, the upper part of the second tube plug 13 of the freeze-drying plug 3 is also vertically inserted into the cylindrical upper part 4 of the tube body 1, the second horizontal annular bulge 21 is abutted against the inner wall of the cylindrical upper part 4 until the second cover plate 12 of the freeze-drying plug 3 is abutted against the top end of the cylindrical upper part 4 and the annular flange 6, as shown in figure 6, thereby effectively ensuring the environmental humidity of freeze-drying reagent packaging, and effectively isolating the external humidity interference in the subsequent transportation and storage process, thereby ensuring the stability of the lyophilized reagent.
During the PCR reaction, the freeze-drying plug 3 is pulled out, then the re-dissolving agent (such as water, or sample preserving fluid, etc.) and the sample (such as nucleic acid) are directly added into the tube body 1, then the PCR cap 2 is covered on the tube body 1, specifically, the first tube plug 8 of the PCR cap 2 is vertically inserted into the cylindrical upper portion 4 of the tube body 1, and abuts against the inner wall of the cylindrical upper portion 4 through the first sealing ring 9 until the first cover plate 7 of the PCR cap 2 abuts against the top end of the cylindrical upper portion 4 and the annular flange 6, and abuts against the inner wall of the cylindrical upper portion 4 through the second sealing ring 17, during the process, the hook portion 11 of the hook 10 is pressed by the annular flange 6 to deform outwards, passes over the annular flange 6, and then is hooked on the bottom surface of the annular flange 6, as shown in fig. 2. Like this, establish on the bottom surface of annular flange 6 through pothook 10 hook, be convenient for carry out the buckle lock with body 1 and die, play sealed body 1's purpose, prevent that the unexpected aerosol that leads to uncaps from polluting, in addition, support through first sealing washer 9 and second sealing washer 17 and lean on the inner wall of cylindrical upper portion 4 to seal body 1, avoid PCR reaction in-process liquid to spill over and the gas volatilizees.
Therefore, the reagent freeze-drying and PCR reaction one-tube operation can be realized, the vertical groove is formed in the outer side surface of the lower part of the second tube plug of the freeze-drying plug, so that the freeze-drying plug is not compacted during freeze-drying, one half of the freeze-drying plug is kept outside to play a role of a cover, the gas inside can be ensured to flow out, the freeze-drying plug is compacted after freeze-drying, the environment humidity for packaging the freeze-drying reagent is effectively ensured, and the external humidity interference can be effectively isolated in the subsequent transportation and storage processes, so that the stability of the freeze-drying reagent is ensured; through the design of the clamping hook, after the operator adds the complex solvent and the sample, the PCR cover can not be opened again, thereby fundamentally putting an end to the potential hidden danger of secondary cover opening operation, effectively reducing the probability of pollution occurrence, and in addition, the sealing ring, especially the double sealing ring, is adopted for sealing, thereby effectively preventing the product from being leaked, and further avoiding the product pollution.
In conclusion, the tube assembly for carrying out reagent freeze-drying and PCR reaction in one tube type can realize one-tube operation from reagent freeze-drying to PCR reaction, can be used for reagent freeze-drying, meets the freeze-drying requirement, is suitable for long-distance transportation, can be directly used for PCR reaction, reduces reagent loss and material consumption cost in the detection process, prevents liquid overflow and gas volatilization in the PCR reaction process, avoids pollution, and is ingenious in design, simple in structure, simple and convenient to manufacture, low in cost and suitable for large-scale popularization and application.
It will thus be seen that the objects of the utility model have been fully and effectively accomplished. The functional and structural principles of the present invention have been shown and described in the embodiments, and the embodiments may be modified without departing from the principles. Therefore, this invention includes all modifications encompassed within the spirit and scope of the claims.
Claims (10)
1. The utility model provides a pipe assembly for one tubular carries out reagent freeze-drying and PCR reaction, includes the body, the vertical setting of body, the body includes cylindrical upper portion, obconic lower part and annular flange, cylindrical upper portion sets up on the obconic lower part, annular flange level cover is established the lateral surface on the top of cylindrical upper portion, its characterized in that, a pipe assembly for one tubular carries out reagent freeze-drying and PCR reaction still include PCR lid and freeze-drying stopper, wherein:
the PCR cover comprises a first cover plate, a first pipe plug and a clamping hook, wherein the first pipe plug is vertically arranged, the first cover plate is horizontally arranged on the first pipe plug, the clamping hook is vertically arranged and positioned below the first cover plate, the upper end of the clamping hook is connected with the first cover plate, the lower end of the clamping hook is a hook part, the hook part is arranged towards the first pipe plug, the first pipe plug is vertically inserted into the cylindrical upper part, the outer side surface of the first pipe plug abuts against the inner wall of the cylindrical upper part, the first cover plate abuts against the top end of the cylindrical upper part and the annular flange, the middle part of the clamping hook is positioned outside the annular flange, and the hook part is hooked on the bottom surface of the annular flange;
the freeze-drying plug comprises a second cover plate and a second pipe plug, the second pipe plug is vertically arranged, the second cover plate is horizontally arranged on the second pipe plug, the second pipe plug is used for being vertically inserted into the cylindrical upper portion, the outer side face of the second pipe plug is used for abutting against the inner wall of the cylindrical upper portion, and the second cover plate is used for abutting against the top end of the cylindrical upper portion and the annular flange.
2. The tube assembly for tube-wise reagent lyophilization and PCR reaction of claim 1, wherein the PCR cap further comprises a first sealing ring horizontally disposed on an outer side of the lower end of the first stopcock and abutting against an inner wall of the cylindrical upper portion.
3. The tube assembly for one-tube reagent lyophilization and PCR reaction according to claim 2, wherein a first annular groove is formed on an outer side surface of the lower end of the first tube stopper along a circumferential direction of the first tube stopper, and the first sealing ring is horizontally inserted into the first annular groove.
4. The tube assembly of claim 1, wherein the plurality of hooks are spaced around the first plug.
5. The tube assembly for tube-wise reagent lyophilization and PCR reaction of claim 1, wherein the PCR cap further comprises a second sealing ring horizontally fitted over an outer side of the upper end of the first plug and abutting against an inner wall of the cylindrical upper portion and below the annular flange.
6. The tube assembly for one-tube-type reagent lyophilization and PCR reaction according to claim 5, wherein a second annular groove is formed on an outer side surface of an upper end of the first tube stopper along a circumferential direction of the first tube stopper, and the second sealing ring is horizontally inserted into the second annular groove.
7. The tube assembly for one-tube reagent lyophilization and PCR reaction of claim 1, wherein the PCR lid further comprises a grip disposed on the first cover plate.
8. The tube assembly for one-tube reagent lyophilization and PCR reaction according to claim 1, wherein the outer side surface of the lower portion of the second tube stopper is provided with a vertical groove, the lower end of the vertical groove is an opening, and the opening is provided on the bottom surface of the second tube stopper.
9. The tube assembly for tube-type reagent lyophilization and PCR reaction of claim 8, wherein the outer side of the lower portion of the second plug has a first horizontal annular protrusion, the vertical groove is disposed in the first horizontal annular protrusion, and the first horizontal annular protrusion is configured to abut against the inner wall of the cylindrical upper portion.
10. The tube assembly for tube-wise reagent lyophilization and PCR reaction of claim 1, wherein an outer side of the upper portion of the second plug has a second horizontal annular protrusion for abutting against an inner wall of the cylindrical upper portion.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122568976.7U CN216550413U (en) | 2021-10-25 | 2021-10-25 | Tube assembly for one-tube reagent freeze-drying and PCR reaction |
| PCT/CN2022/127426 WO2023072090A1 (en) | 2021-10-25 | 2022-10-25 | Polymerase chain reaction tube assembly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122568976.7U CN216550413U (en) | 2021-10-25 | 2021-10-25 | Tube assembly for one-tube reagent freeze-drying and PCR reaction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216550413U true CN216550413U (en) | 2022-05-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122568976.7U Active CN216550413U (en) | 2021-10-25 | 2021-10-25 | Tube assembly for one-tube reagent freeze-drying and PCR reaction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216550413U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023072090A1 (en) * | 2021-10-25 | 2023-05-04 | 上海透景生命科技股份有限公司 | Polymerase chain reaction tube assembly |
-
2021
- 2021-10-25 CN CN202122568976.7U patent/CN216550413U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023072090A1 (en) * | 2021-10-25 | 2023-05-04 | 上海透景生命科技股份有限公司 | Polymerase chain reaction tube assembly |
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