CN219991537U - Anti-pollution eight-row PCR tube - Google Patents
Anti-pollution eight-row PCR tube Download PDFInfo
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- CN219991537U CN219991537U CN202321008106.7U CN202321008106U CN219991537U CN 219991537 U CN219991537 U CN 219991537U CN 202321008106 U CN202321008106 U CN 202321008106U CN 219991537 U CN219991537 U CN 219991537U
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- pcr
- pcr tube
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- connecting strip
- pollution
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- 238000007789 sealing Methods 0.000 claims abstract description 21
- 230000008878 coupling Effects 0.000 claims description 7
- 238000010168 coupling process Methods 0.000 claims description 7
- 238000005859 coupling reaction Methods 0.000 claims description 7
- 230000002265 prevention Effects 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 9
- 238000006243 chemical reaction Methods 0.000 abstract description 7
- 239000012295 chemical reaction liquid Substances 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 2
- 238000012864 cross contamination Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000011324 bead Substances 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011403 purification operation Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 206010064571 Gene mutation Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- Devices For Use In Laboratory Experiments (AREA)
Abstract
The utility model discloses an anti-pollution eight-row PCR tube, which relates to the field of medical experimental equipment and comprises a PCR tube body and a sealing assembly, wherein a limit groove is formed in the top of the PCR tube body, the outer surface of the PCR tube body is fixedly connected with a connecting assembly, the side of the connecting assembly is fixedly provided with a flexible connecting strip, and the connecting assembly comprises a first connecting ring, an arc-shaped connecting strip and a second connecting ring. This anti-pollution eight link row PCR pipes, the volume is great, can hold in the PCR pipe is 300ul, not only can be used to 100ul PCR reaction liquid at the PCR in-process reaction, also can be used to the low reaches experiment of great volume demand, the circumstances that the lock catch design has avoided the unexpected uncapping appears and the leakproofness has obtained further promotion simultaneously, and the arc buckle of lid bottommost design can further improve the joint strength between lid and the body, when putting into the outside no side force interference of reaction liquid, the lid is difficult to open, the tight seam of lid and body has been strengthened in addition in the buckle design has increased the leakproofness of PCR pipe.
Description
Technical Field
The utility model relates to the field of medical experimental equipment, in particular to an anti-pollution eight-row PCR tube.
Background
The polymerase chain reaction technology is widely applied to molecular biology experiments such as detecting gene mutation, the PCR reaction needs to be completed in a PCR experimental device, the device is called a PCR tube, eight rows of PCR tubes are invented for higher throughput completion experiments, the common eight rows of PCR tubes consist of a tube body, a cover body and a connector, the tube body and the cover body are connected together, and the cover body can be provided with hollow cylindrical protrusions and can also be planar, however, the eight rows on the market at present mainly have the following problems:
first, the maximum volume of the eight-row PCR tube is not more than 220ul, but in some experiments, the reaction volume is more than 220ul, for example, in the magnetic bead sorting step in library construction, if the reaction volume is more than 220ul in the magnetic bead sorting step, the eight-row PCR tube cannot be used for purification operation, and the reaction solution needs to be placed in a 1.5ml centrifugal single tube, so that the purification operation cannot be performed by using a multichannel pipettor, and the purification efficiency is low.
Secondly, the tube body and the cover body are not sealed enough, accidental uncovering can not be effectively avoided, after the PCR reaction is finished, however, the operation that some experiments follow is boiling water bath, freezing, incubation, sample storage and the like, so that looseness and even direct falling-off occur between the tube body and the cover body easily, and the interference is brought to the follow-up experimental operation of experimental staff.
Thirdly, most of the eight-row PCR tubes on the market are in a tube body and cover body separation mode, and even if the eight-row PCR tubes with the tube body connected with the cover body exist, cross contamination is easy to cause when the cover is opened.
Therefore, the utility model provides an anti-pollution eight-row PCR tube which is researched and improved aiming at the defects of the existing structure.
Disclosure of Invention
The utility model aims to provide an anti-pollution eight-row PCR tube so as to solve the problems in the background technology.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides an anti-pollution eight link row PCR pipes, includes PCR body and seal assembly, the spacing groove has been seted up at the top of PCR body, and the surface fixedly connected with coupling assembling of PCR body to coupling assembling's avris fixed mounting has soft connecting strip, coupling assembling includes first go-between, arc connecting strip and second go-between, and the avris fixedly connected with arc connecting strip of first go-between, and the other end fixed mounting of arc connecting strip has the second go-between, and the inside of first go-between and the surface fixed connection of PCR body, the inside of second go-between and the surface fixed connection of another PCR body simultaneously, seal assembly fixed mounting is on the other end of soft connecting strip, and seal assembly includes connecting plate, sealed lid and arc hasp, and the top fixedly connected with sealed lid of connecting plate, and the top fixedly connected with arc hasp of connecting plate.
Further, the height of the PCR tube body is 21.8 cm, the internal height is 21.3 cm, and the internal diameter of the PCR tube body is 5.9 cm.
Further, the end of the arc-shaped connecting strip is fixedly connected with the side of the first connecting ring, the other end of the arc-shaped connecting strip is fixedly connected with the side of the second connecting ring, and the second connecting ring and the first connecting ring form a fixed structure through the arc-shaped connecting strip.
Further, the side of the connecting plate is fixedly connected with the end part of the flexible connecting strip, the other end of the flexible connecting strip is fixedly connected with the side of the PCR tube body, and the connecting plate and the PCR tube body form a fixed structure through the flexible connecting strip.
Further, the external dimension of the sealing cover is completely matched with the internal dimension of the limiting groove, and the sealing cover and the PCR tube body form a clamping structure through the limiting groove.
Further, the inner surface of the arc-shaped lock catch is connected with the side of the first connecting ring in a fitting mode, and the arc-shaped lock catch and the first connecting ring form a clamping structure.
The utility model provides an anti-pollution eight-row PCR tube, which has the following beneficial effects:
1. the utility model has larger volume, the volume which can be contained in the PCR tube is 300ul, the utility model not only can be used for the reaction of 100ul PCR reaction liquid in the PCR process, but also can be used for the downstream experiment with larger volume requirement, meanwhile, the lock catch design avoids the occurrence of unexpected uncovering and further improves the tightness, the arc-shaped buckle designed at the bottommost end of the cover body can further improve the connection strength between the cover body and the tube body, when the reaction liquid is put in, the cover body is not easy to open when the external lateral force is not interfered, the buckle design strengthens the tight seam between the cover body and the tube body, increases the tightness of the PCR tube, thereby effectively enhancing the pollution prevention effect of the PCR tube, and the connecting component is in a chain-shaped soft connection state, so that the liquid in the PCR tube can be effectively prevented from splashing out under the uncovering state (relative to the tube without soft connection), thereby further increasing the pollution prevention effect of the PCR tube, and simultaneously, the connecting component can well fix eight tube bodies on the same horizontal plane and the fixed tube body cannot break.
2. According to the utility model, each tube body is connected with an independent cover body, and the cover bodies are at a certain inclination angle, so that the occurrence of cross contamination is effectively avoided, the cover bodies and the tube bodies are at an included angle of 50 degrees, the cross contamination can be effectively avoided, the PCR centrifuge tube with the lock catch can provide the same safety as a single cover and the same convenience as a plurality of covers, can be safely used for boiling water bath, freezing, incubation and sample storage, and is suitable for important and routine experiments.
Drawings
FIG. 1 is a schematic perspective view of a PCR tube body-connecting assembly of an anti-pollution eight-row PCR tube;
FIG. 2 is a schematic top view of an anti-pollution eight-row PCR tube;
FIG. 3 is a schematic side view of a single tube of an anti-pollution eight-row PCR tube;
FIG. 4 is a schematic diagram of the front view structure of an anti-pollution eight-row PCR tube;
FIG. 5 is a schematic diagram showing the connection of the arc-shaped lock catch and the first connecting ring of the anti-pollution eight-row PCR tube.
In the figure: 1. a PCR tube body; 2. a limit groove; 3. a connection assembly; 31. a first connection ring; 32. an arc-shaped connecting strip; 33. a second connecting ring; 4. a flexible connecting strip; 5. a seal assembly; 51. a connecting plate; 52. sealing cover; 53. and (5) arc-shaped lock catches.
Description of the embodiments
Embodiments of the present utility model are described in further detail below with reference to the accompanying drawings and examples. The following examples are illustrative of the utility model but are not intended to limit the scope of the utility model.
As shown in figures 1, 2 and 4, an anti-pollution eight-row PCR tube comprises a PCR tube body 1 and a sealing assembly 5, wherein a limit groove 2 is formed in the top of the PCR tube body 1, the height of the PCR tube body 1 is 21.8 cm, the internal height is 21.3 cm, the internal diameter of the PCR tube body 1 is 5.9 cm, more detection solution can be filled in the PCR tube body 1, the outer surface of the PCR tube body 1 is fixedly connected with a connecting assembly 3, the connecting assembly 3 comprises a first connecting ring 31, an arc connecting strip 32 and a second connecting ring 33, the side of the first connecting ring 31 is fixedly connected with the arc connecting strip 32, the end part of the arc connecting strip 32 is fixedly connected with the side of the first connecting ring 31, the other end of the arc connecting strip 32 is fixedly connected with the side of the second connecting ring 33, and the second link 33 forms fixed knot through arc connecting strip 32 and first link 31 constructs fixed knot through setting up to fixed knot constructs second link 33 and first link 31 for the laboratory personnel can not appear the phenomenon that drops when taking this eight links row PCR pipes any one PCR body 1, and the other end fixed mounting of arc connecting strip 32 has second link 33, and the inside of first link 31 and the surface fixed connection of PCR body 1, the inside of second link 33 and the surface fixed connection of another PCR body 1 simultaneously, through the arc connecting strip 32 that sets up, make the connection between first link 31 and the second link 33 more firm, and whole aesthetic property is stronger, and coupling assembling 3 avris fixed mounting has soft connecting strip 4, sealing component 5 fixed mounting is on the other end of soft connecting strip 4.
As shown in fig. 1-4, a limit groove 2 is formed at the top of the PCR tube body 1, a connecting component 3 is fixedly connected to the outer surface of the PCR tube body 1, a flexible connecting strip 4 is fixedly arranged at the side of the connecting component 3, a sealing component 5 is fixedly arranged at the other end of the flexible connecting strip 4, the sealing component 5 comprises a connecting plate 51, a sealing cover 52 and an arc-shaped lock catch 53, the sealing cover 52 is fixedly connected to the top of the connecting plate 51, the outer dimension of the sealing cover 52 is completely matched with the inner dimension of the limit groove 2, the sealing cover 52 and the PCR tube body 1 form a clamping structure through the limit groove 2, the sealing cover 52 and the PCR tube body 1 which are arranged into the clamping structure are convenient for an experimenter to cover the PCR tube body 1, the side of the connecting plate 51 is fixedly connected with the end part of the flexible connecting strip 4, the other end of the flexible connecting strip 4 is fixedly connected with the side of the PCR tube body 1, the connecting plate 51 and the PCR tube body 1 form a fixed structure through the flexible connecting strip 4, the connecting plate 51 and the PCR tube body 1 which are arranged into the fixed structure ensure that an experiment person can not separate the connecting plate 51 from the PCR tube body 1 when the experiment person pulls the connecting plate 51 by accident, the arc-shaped lock catch 53 is fixedly arranged at the top of the connecting plate 51, the inner surface of the arc-shaped lock catch 53 is in fit connection with the side of the first connecting ring 31, the arc-shaped lock catch 53 and the first connecting ring 31 form a clamping structure, the arc-shaped lock catch 53 is conveniently clamped on the side of the first connecting ring 31 through the arc-shaped lock catch 53 and the first connecting ring 31 which are arranged into the clamping structure, so that the sealing property and the locking effect of the sealing cover 52 are further enhanced, so that the sealability between the sealing cap 52 and the PCR body 1 is further enhanced.
To sum up, according to the structure shown in fig. 1 to 4, firstly, experimental materials are respectively injected into the PCR tube body 1 by an experimenter, then the connecting plate 51 is grasped and the sealing cover 52 is driven to move towards the top of the PCR tube body 1 by the matching of the soft connecting strip 4, then the sealing cover 52 is clamped at the top of the PCR tube body 1 by the matching of the limiting groove 2, then the arc-shaped lock catch 53 is continuously pressed, so that the arc-shaped lock catch 53 is clamped on the side of the first connecting ring 31, the purpose of further locking the sealing cover 52 is achieved, experimental materials are injected into the other seven PCR tube bodies 1 in the same way, then the experimenter can simultaneously take up the eight PCR tube bodies 1 together by the matching of the first connecting ring 31, the arc-shaped connecting strip 32 and the second connecting ring 33, and finally the experimenter puts the PCR tube together with the experimental materials in the PCR tube to carry out subsequent operation detection.
The embodiments of the utility model have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the utility model in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments were chosen and described in order to best explain the principles of the utility model and the practical application, and to enable others of ordinary skill in the art to understand the utility model for various embodiments with various modifications as are suited to the particular use contemplated.
Claims (6)
1. The utility model provides an anti-pollution eight link row PCR pipes, includes PCR body (1) and seal assembly (5), a serial communication port, spacing groove (2) have been seted up at the top of PCR body (1), and the surface fixedly connected with coupling assembling (3) of PCR body (1), and the avris fixed mounting of coupling assembling (3) has soft connecting strip (4), coupling assembling (3) are including first go-between (31), arc connecting strip (32) and second go-between (33), and avris fixedly connected with arc connecting strip (32) of first go-between (31), and the other end fixed mounting of arc connecting strip (32) has second go-between (33), and the inside of first go-between (31) and the surface fixedly connected with of PCR body (1), the surface fixedly connected with of the inside of second go-between (33) and another PCR body (1), seal assembly (5) fixed mounting is on the other end of soft connecting strip (4), and seal assembly (5) are including connecting plate (51), sealed lid (52) and hasp (53), and arc connecting plate (51) are fixed connection top (53).
2. The eight-row PCR tube for pollution prevention according to claim 1, wherein the height of the PCR tube body (1) is 21.8 cm, the internal height is 21.3 cm, and the internal diameter of the PCR tube body (1) is 5.9 cm.
3. The anti-pollution eight-row PCR tube according to claim 1, wherein the end part of the arc-shaped connecting strip (32) is fixedly connected with the side of the first connecting ring (31), the other end of the arc-shaped connecting strip (32) is fixedly connected with the side of the second connecting ring (33), and the second connecting ring (33) and the first connecting ring (31) form a fixed structure through the arc-shaped connecting strip (32).
4. The anti-pollution eight-row PCR tube according to claim 1, wherein the side of the connecting plate (51) is fixedly connected with the end part of the flexible connecting strip (4), the other end of the flexible connecting strip (4) is fixedly connected with the side of the PCR tube body (1), and the connecting plate (51) and the PCR tube body (1) form a fixed structure through the flexible connecting strip (4).
5. The anti-pollution eight-row PCR tube according to claim 1, wherein the outer dimension of the sealing cover (52) is completely matched with the inner dimension of the limiting groove (2), and the sealing cover (52) and the PCR tube body (1) form a clamping structure through the limiting groove (2).
6. The anti-pollution eight-row PCR tube according to claim 1, wherein the inner surface of the arc-shaped lock catch (53) is in fit connection with the side of the first connecting ring (31), and the arc-shaped lock catch (53) and the first connecting ring (31) form a clamping structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321008106.7U CN219991537U (en) | 2023-09-13 | 2023-09-13 | Anti-pollution eight-row PCR tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321008106.7U CN219991537U (en) | 2023-09-13 | 2023-09-13 | Anti-pollution eight-row PCR tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219991537U true CN219991537U (en) | 2023-11-10 |
Family
ID=88611858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321008106.7U Active CN219991537U (en) | 2023-09-13 | 2023-09-13 | Anti-pollution eight-row PCR tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219991537U (en) |
-
2023
- 2023-09-13 CN CN202321008106.7U patent/CN219991537U/en active Active
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