CN215856089U

CN215856089U - Detection device with reagent

Info

Publication number: CN215856089U
Application number: CN202122112883.3U
Authority: CN
Inventors: 陈华卿; 薛立新; 潘成功
Original assignee: Zhejiang Zhiyi Medical Technology Co ltd
Current assignee: Zhejiang Zhiyi Medical Technology Co ltd
Priority date: 2021-09-02
Filing date: 2021-09-02
Publication date: 2022-02-18
Anticipated expiration: 2031-09-02

Abstract

The utility model provides a detection device filled with a reagent, and belongs to the technical field of detection. It has solved the current nucleic acid detection problem with high costs. The detection device with the reagent comprises a test tube, wherein the reagent is arranged in the test tube, the test tube is manufactured by adopting a blow molding process, the test tube comprises a tube body part, a first cover part and a second cover part, the first cover part is connected with the tube body part, and the second cover part is connected with the first cover part; the first cover part is disassembled, an opening is formed on the pipe body part, the disassembled first cover part can be buckled with the pipe body part, the first cover part covers the opening, and the first cover part and the side wall of the opening form sealing; and (4) removing the second cover part, and forming a liquid dropping hole on the first cover part. The test tube of the detection device filled with the reagent is manufactured by adopting a blow molding process, and has the advantages of high production efficiency and low manufacturing cost. Not only have the body portion of depositing reagent and sample in the test tube, still have and cover the uncovered lid of body portion, detection device alright go into reagent in the test tube when leaving the factory like this, and reagent can not leak moreover.

Description

Detection device with reagent

Technical Field

The utility model belongs to the technical field of detection, relates to a detection device, and particularly relates to a detection device filled with a reagent.

Background

The substance for nucleic acid detection is a nucleic acid of a virus. After the new coronavirus infects human body, it will propagate in respiratory tract system, so it can judge whether human body is infected by virus by detecting virus nucleic acid in sputum and nasopharyngeal swab.

The brief process of nucleic acid detection is that firstly, a nasopharynx swab collects a specimen, then the specimen is put into a test tube, an appropriate amount of reagent is put into the test tube, the required reaction time is reduced by shaking, and finally a little reagent is taken out by a dropper for nucleic acid detection.

Although conventional detection devices and detection methods can detect nucleic acids, those skilled in the art would expect to reduce the cost of viral nucleic acid detection.

Disclosure of Invention

The utility model provides a detection device filled with a reagent, and the technical problem to be solved by the utility model is how to reduce the detection cost of nucleic acid.

The technical problem to be solved by the utility model can be realized by the following technical scheme: the detection device is characterized in that the test tube is manufactured by adopting a blow molding process, and comprises a tube body part, a first cover part and a second cover part, wherein the first cover part is connected with the tube body part, and the second cover part is connected with the first cover part; the first cover part is disassembled, an opening is formed on the pipe body part, the disassembled first cover part can be buckled with the pipe body part, the first cover part covers the opening, and the first cover part and the side wall of the opening form sealing; and (4) removing the second cover part, and forming a liquid dropping hole on the first cover part.

Compared with the prior art, the test tube of the detection device filled with the reagent is manufactured by adopting a blow molding process, and has the advantages of high production efficiency and low manufacturing cost. Not only have the body portion of depositing reagent and sample in the test tube, still have and cover the uncovered lid of body portion, detection device alright go into reagent in the test tube when leaving the factory like this, and reagent can not leak moreover.

By illustrating the use method of detecting nucleic acid by using the detection device, the functions and advantages of all the parts are explained, the first step of removing the first cover part ensures that the test tube is erected when the first cover part is removed, the reagent is positioned in the tube body part, and the reagent is prevented from being lost. A second step of sample loading, namely placing the nasopharyngeal swab sample into the tube body and fastening the first cover part on the tube body, so that the possibility of reagent splashing is remarkably reduced, namely the detection device in the state is still suitable for long-distance transfer, such as transportation from a collection site to a detection laboratory through a vehicle; meanwhile, the sample is immersed in the reagent in the state, and the sample reacts with the reagent in the transportation process, so that the storage time of a laboratory is reduced, and the time required by detection is shortened. Thirdly, taking out a small amount of reagent, removing the second cover part and the inverted test tube, and dripping the reagent in a droplet shape from the dripping hole; the size of the drip hole is adjusted according to the viscosity of the reagent and the test tube is extruded adaptively.

In summary, the use of the detection device for detecting nucleic acids reduces the cost of nucleic acid detection by reducing the cost of manufacturing the test tube and eliminating the need for pipetting reagents. The detection of nucleic acids using the detection device also reduces the length of time required for detection. The detection device filled with the reagent is particularly suitable for being applied to large-scale personnel nucleic acid detection scenes.

In the above detection device containing a reagent, the tube body has a housing section, a partial area of a side wall of the housing section is a pressing area protruding outward, and the wall thickness of the pressing area is smaller than that of the rest area of the housing section.

In the above-described detection device containing a reagent, one end of the first cover portion has a first connection section extending obliquely inward, the thickness of the first connection section gradually decreases from the outside to the inside, and the inner end of the first connection section is connected to the tube body portion.

In the above detection device with a reagent, the thinnest part of the first connecting section has a thickness of 0.15mm-0.25 mm.

In the above detection device containing a reagent, the body portion has a neck-reducing section, the first connection section is connected to the neck-reducing section, and the diameter of the port in the first connection section is smaller than that of the neck-reducing section.

In the above-mentioned detecting device with reagent, the neck section has an annular limiting region protruding outwards, and pressing the first cover portion can force the inner end of the first connecting section to pass through the annular limiting region.

In the above-described detection apparatus containing a reagent, one end of the second cover portion has a second connection section extending obliquely inward, the thickness of the second connection section gradually decreases from outside to inside, and the second connection section of the second cover portion is connected to the other end of the first cover portion.

In the above device for detecting containing reagent, the test tube further comprises a sheet-shaped side ear portion, and the inner side of the side ear portion is connected to both the neck-reduced section of the tube body portion and the first cover portion.

In the above-described detection apparatus containing a reagent, the thickness of the region of the side ear portion connected to the tube body portion is gradually reduced from the outside to the inside.

Drawings

FIG. 1 is a schematic perspective view of a detecting unit containing a reagent.

FIG. 2 is a schematic front view of the detecting unit containing the reagent.

Fig. 3 is a schematic sectional structure view of a-a in fig. 2.

Fig. 4 is an enlarged view of a portion C of fig. 3.

Fig. 5 is an enlarged view of a portion of the structure at D in fig. 3.

Fig. 6 is a schematic sectional structure view of B-B in fig. 2.

Fig. 7 is a schematic view showing a structure in which the detached cap portion is fastened to the body portion.

Fig. 8 is a schematic structural view in an inverted state with the second cover portion removed.

In the figure, 1, a tube body part; 1a, opening the mouth; 1b, a containing section; 1c, a necking section; 1d, a pressing area; 1e, an annular limiting area; 2. a first cover portion; 2a, a liquid dropping hole; 2b, a first connecting section; 2c, a reduction section; 2d, an annular raised area; 3. a second cover portion; 3a, a handle area; 3b, a second connecting section; 4. a first annular groove; 5. a second annular groove; 6. a side ear; 20. and (3) a reagent.

Detailed Description

The following are specific embodiments of the present invention and are further described with reference to the drawings, but the present invention is not limited to these embodiments.

As shown in fig. 1 to 6, the detecting unit includes a test tube and a reagent 20 contained in the test tube. The detecting device is used for detecting nucleic acid, and then the reagent 20 is a nucleic acid detecting reagent 20, such as lysis solution or modified magnetic beads, see CN101481400B, CN101665785B, CN 110129312A.

The test tube is made by adopting a blow molding process and is of an integrated structure. The test tube is from supreme being body portion 1, first lid 2 and the second lid 3 in proper order down. The first cover part 2 is disassembled, an opening 1a is formed on the tube body part 1, and the disassembled first cover part 2 can be buckled with the tube body part 1 and cover the opening 1 a; the second cover part 3 is removed and the first cover part 2 is formed with a drip hole 2 a.

After the body portion 1 is blow molded, the reagent 20 is put into the test tube, and the test tube is finally sealed, so that the reagent 20 is prevented from leaking and losing, and a sheet-shaped knob area 3a is formed in the second lid portion 3. The handle area 3a is convenient for the experimenter to hold with fingers, and then detach the second cover part 3 by snapping or rotating, and of course, the second cover part 3 can also be detached by cutting with a cutter.

The tube body part 1 is sequentially provided with an accommodating section 1b and a neck-reducing section 1c from bottom to top, the neck-reducing section 1c is provided with an opening 1a, and the reagent 20 and the sample enter the accommodating section 1b through the opening 1 a. The partial area of the side wall of the containing section 1b protrudes outwards, the protruding area is called as a pressing area 1d, and as the material for forming the pressing area 1d in the tube blank is stretched to be larger, the wall thickness of the pressing area 1d is smaller than that of the rest area of the containing section 1b, namely, the pressing area 1d is easier to be pressed and deformed, so that the volume reduction of the inner cavity of the tube body part 1 is convenient to control, and the agent 20 is better controlled to be discharged from the drip holes 2a at the drip intervals.

One end of the first cover portion 2 has a first connection section 2b extending obliquely inward, and the first connection section 2b of the first cover portion 2 is connected to the neck section 1c of the pipe body portion 1. The wall thickness of the first connecting section 2b can be controlled by controlling the inward inclined extension length of the first connecting section 2b, and the wall thickness of the first connecting section 2b is gradually reduced from outside to inside as the material for forming the first connecting section 2b in the tube blank is stretched to be longer, so that the wall thickness of the first connecting section 2b is smaller than the rest area of the first cover part 2; the thinnest part of the first connecting section 2b is 0.15mm-0.25mm in thickness; that is, the first connecting section 2b is more easily torn or cut at the thinnest portion, thereby facilitating detachment of the first cover portion 2 from the body portion 1.

The diameter of the inner port of the first connecting section 2b is smaller than that of the neck section 1c, and when the first cover part 2 is sleeved on the neck section 1c, the soft first connecting section 2b is equivalent to a sealing lip, so that the sealing performance between the first cover part 2 and the pipe body part 1 is improved.

The neck section 1c has an annular limiting region 1e protruding outward, and pressing the first cover 2 can force the inner end of the first connecting section 2b to cross the annular limiting region 1e, so as to prevent the first cover 2 from falling off naturally and prevent the first cover 2 from falling off when the pressing region 1d is pressed. The annular limiting area 1e is close to the top surface, a first annular groove 4 is formed between the annular limiting area 1e and the first connecting section 2b, and when the first cover part 2 is detached in a snapping or screwing mode, the annular limiting area is usually torn at the bottom surface of the first annular groove 4 and has a smoother tearing edge; when the cutter is used for cutting, the cutter is embedded into the first annular groove 4, so that the cutting stability of the cutter is improved; the outer side surface of the annular limiting region 1e also has a guiding function, so that the first cover part 2 is sleeved on the neck section 1c more easily, and the first cover part 2 is prevented from being embedded into the neck section 1 c.

The first cap portion 2 has a tapered section 2c in a conical shape, which is advantageous in forming a small dropping hole 2a and ensuring smooth flow of the reagent 20 when discharged.

One end of the second cover part 3 is provided with a second connecting section 3b which extends obliquely inwards, the thickness of the second connecting section 3b is gradually reduced from outside to inside, and the second connecting section 3b of the second cover part 3 is connected with the other end of the first cover part 2; the thinnest part of the second connecting section 3b is 0.2mm-0.3mm in thickness. The first lid portion 2 has an annular convex region 2d protruding outward, and a second annular groove 5 is formed between the annular convex region 2d and the second coupling section 3 b. The above structure serves to improve the possibility of tearing at the second annular groove 5 when the second lid portion 3 is broken or screwed.

The test tube is still including being flaky side ear 6, and the inboard of side ear 6 is connected with necking down section 1c and first cap 2 homogeneous phase of barrel 1, and side ear 6 realizes improving the joint strength of barrel 1 and first cap 2, reduces the unexpected possibility of tearing of test tube in barrel 1 and first cap 2 junction. The figures in the specification show that the number of the side ears 6 is one, and the number of the side ears 6 can be two according to actual conditions. The thickness of the connecting area of the side ear part 6 and the tube body part 1 is gradually reduced from outside to inside, so that the connecting area of the side ear part 6 and the tube body part 1 and the first cover part 2 is small in thickness, and the side ear part 6 can be detached by being broken.

The method for detecting nucleic acid by the detection device is carried out according to the following sequence steps, wherein the first step is to remove the first cover part 2, and the test tube is erected when the first cover part 2 is removed; secondly, assembling a sample, namely putting the sample into the tube body 1 from the opening 1a of the tube body 1, sleeving the first cover part 2 on the necking section 1c of the tube body 1, and fastening the first cover part 2 and the tube body 1; and thirdly, extracting the reagent 20, namely removing the second cover part 3, inverting the test tube to enable the dropping hole 2a to face downwards, holding the test tube on the tube body part 1, squeezing the pressing area 1d by using a thumb, reasonably controlling the pressing force to enable the reagent 20 to drop from the dropping hole 2a in a droplet shape, and extracting the reagent 20 with the required dropping number according to actual conditions.

Claims

1. The detection device filled with the reagent comprises a test tube, wherein the reagent (20) is arranged in the test tube, and is characterized in that the test tube is manufactured by adopting a blow molding process, the test tube comprises a tube body part (1), a first cover part (2) and a second cover part (3), the first cover part (2) is connected with the tube body part (1), and the second cover part (3) is connected with the first cover part (2); one end of the first cover part (2) is provided with a first connecting section (2 b) which extends towards the inner side in an inclined mode, the thickness of the first connecting section (2 b) is gradually reduced from the outside to the inside, and the inner end of the first connecting section (2 b) is connected with the pipe body part (1); the first cover part (2) is removed, and an opening (1 a) is formed on the tube body part (1); the first cover part (2) which is disassembled can be buckled with the pipe body part (1), the first cover part (2) covers the opening (1 a), and the first cover part (2) and the side wall of the opening (1 a) form sealing; the second cover part (3) is removed, and a dropping hole (2 a) is formed on the first cover part (2).

2. The reagent-containing test device according to claim 1, wherein the body portion (1) has a housing section (1 b), and a partial region of a side wall of the housing section (1 b) is a pressing region (1 d) protruding outward, and a wall thickness of the pressing region (1 d) is smaller than that of the rest region of the housing section (1 b).

3. The reagent-containing test device according to claim 1, wherein the thinnest portion of the first connecting section (2 b) has a thickness of 0.15mm to 0.25 mm.

4. The reagent-containing test device according to claim 1, wherein the body portion (1) has a neck-down portion (1 c), and the first connecting portion (2 b) is connected to the neck-down portion (1 c), and the diameter of the port in the first connecting portion (2 b) is smaller than the diameter of the neck-down portion (1 c).

5. The reagent-containing test device according to claim 4, wherein the neck section (1 c) has an annular stop region (1 e) projecting to the outside, and pressing the first cap section (2) forces the inner end of the first connecting section (2 b) to pass over the annular stop region (1 e).

6. The reagent-containing measuring device according to any one of claims 1 to 5, wherein one end of the second cover part (3) has a second connecting section (3 b) extending obliquely inward, the thickness of the second connecting section (3 b) is gradually reduced from the outside to the inside, and the second connecting section (3 b) of the second cover part (3) is connected to the other end of the first cover part (2).

7. The reagent-containing detection device according to any one of claims 1 to 5, wherein the cuvette further comprises a side ear (6) in a sheet shape, and an inner side of the side ear (6) is connected to both the neck-reduced section (1 c) of the body portion (1) and the first lid portion (2).

8. The reagent-containing test device of claim 7, wherein the inner region of the side ear (6) tapers in thickness from the outside to the inside.