CN113281145A - Reagent pre-filled storage tube for detection and detection method - Google Patents
Reagent pre-filled storage tube for detection and detection method Download PDFInfo
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- CN113281145A CN113281145A CN202110567043.8A CN202110567043A CN113281145A CN 113281145 A CN113281145 A CN 113281145A CN 202110567043 A CN202110567043 A CN 202110567043A CN 113281145 A CN113281145 A CN 113281145A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/56—Labware specially adapted for transferring fluids
- B01L3/561—Tubes; Conduits
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a reagent pre-filled storage tube for detection and a detection method. The reagent preassembling storage tube for detection comprises a tube body and an edge seal; a liquid outlet section is arranged at the lower section of the pipe body; a first easily-separated part is arranged on the outer side surface of the liquid outlet section; the first easily-separated part extends to the left and the right to the edge sealing respectively; the upper section of the pipe body is provided with an insertion section; the top end of the sealed edge is connected with two stripping sheets. The invention has the beneficial effects that: the reagent pre-filled storage tube for detection has the mixing function and the dripping function, so that a liquid mixing bottle and an extraction tube are not needed in the detection process, and a detection method corresponding to the reagent pre-filled storage tube for detection is developed, so that the number of components in a virus rapid detection kit is reduced, the production cost is reduced, the transfer times of the reagent are reduced, and the virus detection operation steps are simplified.
Description
Technical Field
The invention relates to the field of detection, in particular to a reagent pre-filled storage tube for detection and a detection method.
Background
One of the existing rapid virus detection methods is as follows: firstly, a sample to be detected is collected through a sampling rod, then the collected sample is mixed with a reagent in a matched reagent storage tube to obtain a mixed solution, then a plurality of drops of the mixed solution are dripped into a dripping hole of a matched virus detection box through an extraction tube, and after a certain time period, whether the sample contains corresponding virus or not is judged according to a detection result of the virus detection box.
In the detection process, a reagent storage tube, a liquid mixing bottle and an extraction tube are required to be used at the same time, and the applicant develops a reagent pre-loading storage tube for detection, which has a mixing function and a dropping function at the same time, so that the liquid mixing bottle and the extraction tube are not required to be used in the detection process, and develops a detection method corresponding to the reagent pre-loading storage tube for detection, thereby reducing the number of components in a virus rapid detection kit, lowering the production cost, reducing the transfer times of reagents and simplifying the detection operation steps of viruses.
Disclosure of Invention
In order to solve the problems in the background art, the invention discloses a reagent pre-filled storage tube for detection, which comprises a tube body and an edge sealing; a liquid outlet section is arranged at the lower section of the pipe body; a first easily-separated part is arranged on the outer side surface of the liquid outlet section; the first easily-separated part extends to the left and the right to the edge sealing respectively; the upper section of the pipe body is provided with an insertion section; the top end of the sealed edge is connected with two stripping sheets.
Preferably, a second easily-separated part is arranged on the outer side surface of the insertion section, and the second easily-separated part respectively extends to the left and the right to the sealed edge.
Preferably, the method also comprises blocking; the plugging plug is provided with a plugging part matched with the insertion section.
Preferably, the wall thickness of the first easy-separation part is smaller than that of the liquid outlet section.
Preferably, the cross section of the first easily separable portion is triangular.
Preferably, the wall thickness of the second easily separable portion is smaller than the wall thickness of the pipe body.
Preferably, the cross section of the second easily separable portion is triangular.
Preferably, the front side and the rear side of the pipe body are both provided with a plane.
Disclosed is a detection method, comprising the following steps,
the method comprises the following steps: collecting an object to be detected through a sampling rod;
step two: taking a storage tube for pre-loading the detection reagent according to claim 1 or claim 2, and separating the two stripping sheets towards each other until the sealing edges at the two sides of the insertion section are stripped to expose the insertion section;
step three: inserting the sampling part of the sampling rod into the tube body through the insertion section and stirring the sampling part in the tube body to mix the object to be detected with the reagent in the tube body, and then taking out the sampling rod through the insertion section;
step four: the inserting section or the two stripping sheets are pinched, and then the lower section of the pipe body is separated at the first easy-separation part to expose the liquid outlet section;
step five: and dripping the mixed liquid in the plurality of dropper bodies to a drip hole of a matched detection box through a liquid outlet section, and then waiting for a certain period of time, and judging the property of the object to be detected according to the detection result of the detection box.
Disclosed is a detection method characterized by comprising the steps of,
the method comprises the following steps: collecting an object to be detected through a sampling rod;
step two: taking a reagent prefilled storage tube for testing according to claim 3, and separating the two peeling sheets towards each other until the sealing edges at the two sides of the insertion section are peeled off to expose the insertion section;
step three: inserting the sampling part of the sampling rod into the tube body through the insertion section and stirring the sampling part in the tube body to mix the object to be detected with the reagent in the tube body, and then taking out the sampling rod through the insertion section;
step four: separating the upper section of the pipe body at the second easily-separated part to expose the insertion section, inserting the plugging part of the plugging plug into the insertion section to plug the insertion section, and separating the lower section of the pipe body at the first easily-separated part to expose the liquid outlet section;
step five: and dripping the mixed liquid in the plurality of dropper bodies to a drip hole of a matched detection box through a liquid outlet section, and then waiting for a certain period of time, and judging the property of the object to be detected according to the detection result of the detection box.
The invention has the beneficial effects that: the reagent pre-filled storage tube for detection has the mixing function and the dripping function, so that a liquid mixing bottle and an extraction tube are not needed in the detection process, and a detection method corresponding to the reagent pre-filled storage tube for detection is developed, so that the number of components in a virus rapid detection kit is reduced, the production cost is reduced, the transfer times of the reagent are reduced, and the virus detection operation steps are simplified.
Drawings
FIG. 1 is a schematic view showing the overall structure of a reagent prefilled storage tube for assay of the present invention.
Fig. 2 is a schematic front view of fig. 1.
Fig. 3 is a side view of fig. 1.
Fig. 4 is an enlarged view of a portion a in fig. 3.
Fig. 5 is an enlarged view at B in fig. 3.
Fig. 6 is a schematic structural view of the plug of the present invention.
Fig. 7 is a schematic front view of fig. 6.
Fig. 8 shows one of the states of use of embodiment 1.
Fig. 9 shows one of the states of use of embodiment 1.
FIG. 10 shows one of the states of use of example 2.
Fig. 11 shows one of the states of use of embodiment 2.
Detailed Description
The embodiments of the present invention will be described in detail with reference to the accompanying drawings so that the advantages and features of the invention can be more easily understood by those skilled in the art, and the scope of the invention will be clearly and clearly defined.
In the description of the present invention, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example 1
Referring to fig. 1-5, the reagent prefilled storage tube for detection comprises a tube body 1 and an edge seal 2; a liquid outlet section 3 is arranged at the lower section of the pipe body 1; a first easily-separated part 4 is arranged on the outer side surface of the liquid outlet section 3; the first easily separated part 4 respectively extends to the left and the right to the sealed edge 2; the upper section of the pipe body 1 is provided with an insertion section 5; the top end of the edge sealing 2 is connected with two stripping sheets 6.
The outer side surface of the insertion section 5 is provided with a second easily-separated part 7, and the second easily-separated part 7 respectively extends to the left and the right to the sealed edge 2.
The wall thickness of the first easy-separation part 4 is smaller than that of the liquid outlet section 3.
The cross section of the first easily separated part 4 is triangular.
The thickness of the second easily separable portion 7 is smaller than the thickness of the pipe body 1.
The cross section of the second easily separated part 7 is triangular.
The front side and the rear side of the pipe body 1 are both provided with a plane 101.
Thus, the tube body 1 is flat, so that the reagent pre-filled storage tube for detection can be stacked and placed, and the reagent pre-filled storage tube for detection is convenient to store neatly.
Referring to fig. 8-9, the detection method, includes the steps of,
the method comprises the following steps: collecting the object to be detected through the sampling rod 200;
step two: taking a storage tube for pre-filled detection reagent according to claim 1 or claim 2, separating the two strips 6 towards each other until the sealing edges 2 at the two sides of the insertion section 5 are peeled off to expose the insertion section 5;
step three: inserting the sampling part of the sampling rod into the tube body 1 through the insertion section 5 and stirring in the tube body 1 to mix the object to be detected with the reagent in the tube body 1, and then taking out the sampling rod through the insertion section 5;
step four: the inserting section 5 or the two stripping sheets 6 are pinched, and then the lower section of the pipe body 1 is separated at the first easy separation part 4 to expose the liquid outlet section 3;
step five: the mixed liquid in the plurality of liquid dripping pipe bodies 1 is dripped into the liquid dripping holes of the matched detection box through the liquid outlet section 3, and then after a certain time, the property of the object to be detected is judged according to the detection result of the detection box.
Example 2
Referring to FIGS. 1 to 7, a storage tube for a test reagent is preloaded, and example 2 is different from example 1 in that it further includes a stopper 8; the plug 8 is provided with a plug 801 adapted to the insertion section 5.
Referring to fig. 10-11, the detection method, includes the steps of,
the method comprises the following steps: collecting the object to be detected through the sampling rod 200;
step two: taking a reagent prefilled storage tube for assay according to claim 3, separating the two peel strips 6 towards each other until the sealing edges 2 on both sides of the insertion section 5 are peeled off to expose the insertion section 5;
step three: inserting the sampling part of the sampling rod into the tube body 1 through the insertion section 5 and stirring in the tube body 1 to mix the object to be detected with the reagent 100 in the tube body 1, and then taking out the sampling rod through the insertion section 5;
step four: separating the upper section of the pipe body 1 at the second easily separable part 7 to expose the insertion section 5, inserting the plugging part 801 of the plug 8 into the insertion section 5 to plug the insertion section 5, and separating the lower section of the pipe body 1 at the first easily separable part 4 to expose the liquid outlet section 3;
step five: the mixed liquid in the plurality of liquid dripping pipe bodies 1 is dripped into the liquid dripping holes of the matched detection box through the liquid outlet section 3, and then after a certain time, the property of the object to be detected is judged according to the detection result of the detection box.
Therefore, the reagent pre-filled storage tube for detection has the mixing function and the dripping function, so that a liquid mixing bottle and an extraction tube are not needed in the detection process, and a detection method corresponding to the reagent pre-filled storage tube for detection is developed, so that the number of components in a virus rapid detection kit is reduced, the production cost is reduced, the transfer times of the reagent are reduced, and the virus detection operation steps are simplified.
The invention can be used for, but is not limited to, the detection of new coronaviruses.
The production process of the invention can be realized by the following modes: after the pre-filling of the test reagent prefilled storage tube of the present invention with the reagent is performed through the insertion section 5, only the insertion section 5 and the upper portion of the insertion section 5 are sealed at the time of sealing, thereby forming two peel sheets 6. Thus, the insertion opening of the insertion section 5 can be exposed by pulling the two release sheets 6 outward in opposite directions.
The above description is only a preferred embodiment of the present invention and should not be construed as limiting the present invention, and any minor modifications, equivalent replacements and improvements made to the above embodiment according to the technical spirit of the present invention should be included in the technical scope of the present invention.
Claims (10)
1. The reagent pre-filled storage tube for detection comprises a tube body and an edge sealing; a liquid outlet section is arranged at the lower section of the pipe body; the device is characterized in that a first easily-separated part is arranged on the outer side surface of the liquid outlet section; the first easily-separated part extends to the left and the right to the edge sealing respectively; the upper section of the pipe body is provided with an insertion section; the top end of the sealed edge is connected with two stripping sheets.
2. The pre-filled testing reagent storage tube according to claim 1, wherein a second easily separable portion is provided on the outer surface of the insertion section, and the second easily separable portion extends to the left and right to the seal edge, respectively.
3. The pre-filled storage tube for a test reagent according to claim 2, further comprising a stopper; the plugging plug is provided with a plugging part matched with the insertion section.
4. The preloaded storage tube for a test reagent according to any one of claims 1 to 3, wherein the thickness of the wall of the first easily separable portion is smaller than the thickness of the liquid outlet section.
5. The pre-filled testing reagent storage tube according to claim 4, wherein the first easily separable portion has a triangular cross-section.
6. The preloaded storage tube for a test reagent according to any one of claims 2 to 3, wherein the thickness of the wall at the second easily separable portion is smaller than the thickness of the wall of the tube body.
7. The pre-filled testing reagent storage tube according to claim 6, wherein the second easily separable portion has a triangular cross-section.
8. The pre-filled testing reagent storage tube according to any of claims 1 to 3, wherein the front and rear sides of the tube body are each provided with a flat surface.
9. The detection method is characterized by comprising the following steps,
the method comprises the following steps: collecting an object to be detected through a sampling rod;
step two: taking a storage tube for pre-loading the detection reagent according to claim 1 or claim 2, and separating the two stripping sheets towards each other until the sealing edges at the two sides of the insertion section are stripped to expose the insertion section;
step three: inserting the sampling part of the sampling rod into the tube body through the insertion section and stirring the sampling part in the tube body to mix the object to be detected with the reagent in the tube body, and then taking out the sampling rod through the insertion section;
step four: the inserting section or the two stripping sheets are pinched, and then the lower section of the pipe body is separated at the first easy-separation part to expose the liquid outlet section;
step five: and dripping the mixed liquid in the plurality of dropper bodies to a drip hole of a matched detection box through a liquid outlet section, and then waiting for a certain period of time, and judging the property of the object to be detected according to the detection result of the detection box.
10. The detection method is characterized by comprising the following steps,
the method comprises the following steps: collecting an object to be detected through a sampling rod;
step two: taking a reagent prefilled storage tube for testing according to claim 3, and separating the two peeling sheets towards each other until the sealing edges at the two sides of the insertion section are peeled off to expose the insertion section;
step three: inserting the sampling part of the sampling rod into the tube body through the insertion section and stirring the sampling part in the tube body to mix the object to be detected with the reagent in the tube body, and then taking out the sampling rod through the insertion section;
step four: separating the upper section of the pipe body at the second easily-separated part to expose the insertion section, inserting the plugging part of the plugging plug into the insertion section to plug the insertion section, and separating the lower section of the pipe body at the first easily-separated part to expose the liquid outlet section;
step five: and dripping the mixed liquid in the plurality of dropper bodies to a drip hole of a matched detection box through a liquid outlet section, and then waiting for a certain period of time, and judging the property of the object to be detected according to the detection result of the detection box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110567043.8A CN113281145A (en) | 2021-05-24 | 2021-05-24 | Reagent pre-filled storage tube for detection and detection method |
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| CN202110567043.8A CN113281145A (en) | 2021-05-24 | 2021-05-24 | Reagent pre-filled storage tube for detection and detection method |
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| CN113281145A true CN113281145A (en) | 2021-08-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113604346A (en) * | 2021-09-02 | 2021-11-05 | 浙江仅一医疗科技有限公司 | Detection device with reagent and method for detecting nucleic acid by using detection device |
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| CN113604346B (en) * | 2021-09-02 | 2023-11-17 | 浙江仅一医疗科技有限公司 | Detection device containing reagent and method for detecting nucleic acid using detection device |
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