WO2023179635A1

WO2023179635A1 - Integrated reaction test tube, packaging method therefor, and use method and use thereof

Info

Publication number: WO2023179635A1
Application number: PCT/CN2023/082921
Authority: WO
Inventors: 易小钧; 李孟达; 文志勇; 刁维均; 肖江涛
Original assignee: 中元汇吉生物技术股份有限公司
Priority date: 2022-03-22
Filing date: 2023-03-21
Publication date: 2023-09-28
Also published as: CN114578045A

Abstract

An integrated reaction test tube, a packaging method therefor, and a use method thereof. The integrated reaction test tube comprises a tube body (10) and a tube cover (30), a first cavity (11) for placing a test strip (1) being provided in the tube body (10), a first opening (12) for the test strip (1) to pass through being provided on the top surface of the first cavity (11), a fixing plug (13) being mounted in the first opening (12), and a receding recess (14) matching a first end (2) of the test strip (1) being provided in the fixing plug (13). The integrated reaction test tube is provided with the first cavity (11) for placing the test strip (1) and is provided with the fixing plug (13) in the first opening (12) of the first cavity (11). The fixing plug (13) not only can be used for positioning the first end (2) of the test strip (1), but also can prevent, when the test tube is inverted, a splashing liquid reagent from entering the first cavity (11) and further prevent the splashing liquid reagent from polluting an NC membrane (3) of the test strip (1), thus effectively improving the success rate of chromatography assay.

Description

Integrated reaction detection tube and packaging method, use method and application thereof

This application requires a Chinese invention patent application submitted to the China Patent Office on March 22, 2022, with the application number 202210283768.9, and the application name is "Integrated reaction detection tube and packaging method, use method and application" as priority, which is related to The entire contents of this application are incorporated by reference into this application.

Technical field

This application belongs to the field of immunochromatography detection technology, specifically an integrated reaction detection tube and its packaging method, usage method and application.

Background technique

Immunochromatographic detection technology uses antigen-antibody immunological reactions and chromatographic reactions in the form of dry sheet test paper to achieve the purpose of rapid and accurate color development to detect the analyte. Generally speaking, samples for diagnosis or testing usually have some dangers, such as certain toxicity and infectiousness, and the used test tubes should be disposed of as medical waste. However, although some existing detection tubes can meet the requirements to a certain extent, they still have the following shortcomings:

1) When the detection tube is inverted, the liquid reagent mixed with the sample to be tested flows rapidly under the action of gravity, converting the gravitational potential energy of the liquid reagent into kinetic energy. Under the action of this kinetic energy, the liquid reagent will splash and come into contact with the test strip. NC membrane, causing chromatography detection to fail;

2) After the detection tube is inverted, the test strip is in a completely sealed environment, and the internal vacuum will affect the chromatographic detection effect.

Contents of the invention

In view of this, the purpose of this application is to provide an integrated reaction detection tube and its packaging method, usage method and application, which can effectively improve the success rate of chromatography detection.

In order to achieve the above purpose, this application provides the following technical solutions:

The present invention first proposes an integrated reaction detection tube, which includes a tube body and a tube cover. The tube body is provided with at least one first cavity for placing test strips. The top surface of the first cavity is provided with There is a first opening for the test strip to pass through, and a fixed plug is installed on the first opening, and a relief groove matching the first end of the test strip is provided on the fixed plug; the first There are ventilation holes in the cavity, and the A result observation area is provided on the side wall of the first cavity.

Further, a pressure rib for positioning the second end of the test strip is provided on the side wall of the first cavity.

Further, the top of the pressure rib is provided with a guide slope for guiding the test strip.

Furthermore, a high-gloss convex surface is provided in the result observation area.

Further, the ventilation hole is provided on the bottom surface of the first cavity.

Further, a second cavity for storing liquid reagent is provided in the tube body, and a second opening is provided on the top surface of the second cavity.

Further, the side wall of the second cavity is provided with ribs to facilitate elution of the sample on the swab.

Further, the bottom of the second cavity is provided with a closing section, the inner diameter of the closing section gradually decreases along the direction from top to bottom, and the bottom of the closing section is provided with injection molding ridges.

Further, the second cavity includes a lower cavity located at the lower part and a transition section located at the upper part, the second opening is square, and the inner wall of the lower cavity is cylindrical; the transition section is used to make the second cavity open. There is a smooth connection between the opening and the lower cavity.

Further, the tube cover is provided with a cover groove with an open bottom surface, and the notch of the cover groove forms a cover opening. The tube cover covers the upper end of the tube body and is between the tube cover and the tube body. A third cavity is formed therebetween for communicating with the first cavity and the second cavity.

Furthermore, the outer wall of the pipe body is provided with a limiting convex rib for limiting the position of the pipe cover. The limiting convex rib separates the pipe body into an upper part of the pipe body and a lower part of the pipe body. The pipe cover covers On the upper part of the tube body; the axial length of the upper part of the tube body is less than the depth of the cover groove.

Further, a sealing structure is provided between the pipe body and the pipe cover; the sealing structure adopts a sealing rib provided between the pipe cover and the limiting rib; the sealing rib is provided on the pipe cover. on the bottom surface, or the sealing rib is arranged on the upper side of the limiting convex rib.

Further, the upper part of the pipe body is provided with a reducing section for interference fit with the pipe cover at a position close to the limiting rib; any two planes parallel to each other and perpendicular to the axis of the pipe body are located there. The cross-sections taken on the variable diameter section are similar in graphics, and the geometric size of the cross-section taken on the lower plane is larger than the geometric size of the cross-section taken on the upper plane.

Further, a sealing structure is provided between the pipe body and the pipe cover; the sealing structure includes matching sealing ribs and sealing grooves; the sealing ribs are arranged on the outer wall of the upper part of the pipe body, and the The sealing groove is provided on the side wall of the cover groove; or, the sealing groove is provided on the outer wall of the upper part of the tube body, and the sealing rib is provided on the side wall of the cover groove.

Furthermore, a positioning structure is provided between the pipe body and the pipe cover.

Further, the positioning structure includes a positioning protrusion and a positioning groove provided in one-to-one correspondence with the positioning protrusion; the positioning protrusion is provided on the outer wall of the upper part of the tube body, and the positioning groove is provided on the outer wall of the upper part of the pipe body. on the side wall of the cover groove; or, the positioning groove is provided on the outer wall of the upper part of the pipe body, and the positioning protrusion is provided on the side wall of the cover groove.

Further, a sealing structure is provided between the pipe body and the pipe cover, and the sealing structure includes mutually matching sealing ribs and sealing grooves;

The side wall of the cover groove includes an upper section of the side wall located at the upper part and a lower section of the side wall located at the lower part. The geometric size of the lower section of the side wall is larger than the geometric size of the upper section of the side wall. The positioning protrusion or positioning groove is provided On the lower section of the side wall, the sealing rib or sealing groove is provided on the upper section of the side wall.

Further, the bottom of the cover groove is provided with a tube cover protrusion that protrudes downward to reduce the volume of the third cavity.

The present invention also proposes a packaging method for the integrated reaction detection tube as described above, which includes the following steps:

1) After injecting the liquid reagent into the second cavity, affix a sealing film on the second opening; then place a test strip in the first cavity and make the first end of the test strip extend out of the first cavity. to open one's mouth;

2) Place the tube cap on the tube body.

Further, in step 2), before placing the tube cover on the tube body, a desiccant is placed in the third cavity.

Further, in step 1), after placing the test strip in the first cavity, a fixing plug is installed on the first opening.

The present invention also proposes a method of using the integrated reaction detection tube as described above. A test strip is placed in the first cavity, a liquid reagent is stored in the second cavity, and a sticker is attached to the second opening. There is a sealing film; it includes the following steps:

Step 1: Remove the tube cover from the tube body and tear off the sealing film on the second opening;

Step 2: Insert the sampling swab into the second cavity, mix the sample to be tested with the liquid reagent, break the sampling swab and leave the broken sampling swab in the second cavity;

Step 3: Place the tube cap on the tube body;

Step 4: Invert the integrated reaction detection tube so that the liquid reagent mixed with the sample to be detected passes through the third chamber and contacts the first end of the test strip;

Step 5: Observe the test results through the test strip.

Further, in step one, when the desiccant is placed in the third cavity, remove the tube cover from After removing the tube body, first remove the desiccant, and then tear off the sealing film on the second opening.

The present invention also proposes the application of an integrated reaction detection tube as described above in the immunochromatographic detection of novel coronavirus, influenza A, influenza B, respiratory syncytial virus or rotadenovirus.

The beneficial effects of this application are:

In the integrated reaction detection tube of the present application, a first cavity is provided to place the test strip, and a fixed plug is provided on the first opening of the first cavity. The fixed plug can not only position the first end of the test strip function, and during the inversion process of the detection tube, it can effectively prevent the splashing liquid reagent from entering the first chamber, thereby preventing the splashing liquid reagent from contaminating the NC membrane of the test strip, which can effectively improve the success rate of chromatography detection.

The preferred solution of this application also has the following technical effects:

1) By setting the pressure rib, the second end of the test strip can be positioned to prevent the test strip from shaking, and the test strip can be attached to the side wall of the first cavity to enhance the chromatography effect; in the guidance of the guide slope Under the action, the test strip can be easily placed at the designated position of the first cavity;

2) By arranging a breathable hole in the first cavity, after the detection tube is inverted, the test strip can be placed in the same pressure environment as the outside world, which can effectively improve the effect of chromatography detection; in addition, during the production process, glue can be avoided The insertion of the punching and bending mold causes undesirable defects such as product perforation, and it can meet the requirements of mold ejection;

3) By setting up a second cavity for storing liquid reagents, and setting ribs on the side wall of the second cavity, the scraping effect of the ribs on the swab is used to facilitate the elution of the sample on the swab and improve the sample quality. elution rate;

4) By setting a closing section at the bottom of the second cavity and setting an injection wave point at the bottom of the closing section, the bottom of the second cavity can be made thicker to ensure that the mold is filled with glue during the production process;

5) By providing a transition section to make a smooth transition between the second opening and the lower cavity, it is possible to prevent liquid from hanging at the second opening and facilitate packaging;

6) By setting limit convex ribs on the outer wall of the pipe, the depth of the pipe cover on the pipe body can be limited to ensure the distance between the bottom of the cover groove and the top surface of the pipe body; at the same time, the limit convex ribs can be Sealing ribs are set between the ribs and the bottom surface of the tube cover to seal, to avoid leakage of liquid reagents when the detection tube is inverted; by setting a reducing section on the upper part of the tube body to interfere with the tube cover, the gap between the tube cover and the tube body is ensured the binding force;

7) Of course, the sealing structure can also be a sealing rib and a sealing groove provided between the pipe body and the pipe cover; at the same time, a positioning structure can also be provided between the pipe body and the pipe cover, and the positioning structure includes positioning protrusions and positioning Groove; in order to make it easier for the pipe cap to be inserted into the pipe body and the pipe cap to be removed from the pipe body, the side wall of the cover groove is divided into two sections. The geometric size of the lower section of the side wall is larger than the geometric size of the upper end of the side wall. The positioning structure is It is arranged on the lower section of the side wall, and the sealing structure is arranged on the upper section of the side wall. In this way, when the pipe cover is covered with the pipe body, the lower section of the side wall can easily pass through the sealing structure provided on the upper part of the pipe body and use the positioning structure to achieve positioning. At the same time, the pipe The sealing structure between the cap and the pipe body fits together exactly; when the cap is removed from the pipe body, after overcoming the structural force of the positioning structure and the sealing structure, the lower section of the side wall can easily pass through the seal provided on the upper part of the pipe body. structure and removed smoothly;

8) The bottom of the cover tank is provided with a tube cover protrusion, thereby reducing the volume of the third cavity and reducing the usage amount of liquid reagent.

Description of the drawings

In order to make the purpose, technical solutions and beneficial effects of this application clearer, this application provides the following drawings for explanation:

Figure 1 is a schematic structural diagram of Embodiment 1 of the integrated reaction detection tube of the present application;

Figure 2 is an upper isometric view of Figure 1;

Figure 3 is the lower isometric view of Figure 1;

Figure 4 is a schematic structural diagram of the pipe body in this embodiment;

Figure 5 is a perspective view of the pipe body of this embodiment;

Figure 6 is a schematic structural diagram of the tube cover of this embodiment;

Figure 7 is a perspective view of the tube cover of this embodiment;

Figure 8 is a schematic structural diagram of the fixed plug in this embodiment;

Figure 9 is an exploded view of the integrated reaction detection tube of this embodiment;

Figure 10 is a schematic structural diagram of the test strip;

Figure 11 is a schematic structural diagram of Embodiment 2 of the integrated reaction detection tube of the present application;

Figure 12 is an exploded view of the integrated reaction detection tube of this embodiment;

Figure 13 is a schematic structural diagram of the tube cover of this embodiment.

Explanation of reference symbols:
1-Test strip; 2-First end; 3-NC membrane; 4-Second end;
10-tube body; 10a-upper part of tube body; 10b-lower part of tube body; 11-first cavity; 12-first opening;
13-Fixed plug; 14-Giving groove; 15-Pressure rib; 16-Guide slope; 17-Result observation area; 18-Breathing hole; 19-Second cavity; 19a-Lower cavity; 19b-Transition section; 20 -Second opening; 21-rib; 22-closing section; 23-injection wave point; 24-limiting rib; 25-sealing convex rib; 26-positioning bulge; 27-reducing section;
30-tube cover; 31-cover groove; 31a-upper part of side wall; 31b-lower part of side wall; 32-cover opening; 33-third cavity; 34-sealing groove; 35-positioning groove; 36-tube cover Protrusion; 37-Sealing rib.

Detailed ways

The present application will be further described below with reference to the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the present application and implement it, but the examples are not intended to limit the present application.

Example 1

As shown in Figure 1, it is a schematic structural diagram of Embodiment 1 of the integrated reaction detection tube of the present application. The integrated reaction detection tube of this embodiment includes a tube body 10 and a tube cover 30. The tube body 10 is provided with a first cavity 11 for placing the test strip 1. The top surface of the first cavity 11 is provided with a test strip 1. There is a first opening 12 through which the paper strip 1 passes, and a fixed plug 13 is installed on the first opening 12. The fixed plug 13 is provided with a relief groove 14 that matches the first end 2 of the test strip 1. In the integrated reaction detection tube of this embodiment, a first cavity 11 is provided to place the test strip 1, and a fixed plug 13 is provided on the first opening 12 of the first cavity 11. The fixed plug 13 can not only position the test strip 1 The first end 2 of the paper strip 1 can effectively prevent the splashed liquid reagent from entering the first cavity 11 during the inversion process of the detection tube, thereby preventing the splashed liquid reagent from contaminating the NC film 3 of the test strip. It can effectively improve the success rate of chromatography detection.

Furthermore, a pressure rib 15 for positioning the second end 4 of the test strip 1 is provided on the side wall of the first cavity 11 . By setting the pressure rib 15, the second end 4 of the test strip 1 can be positioned to prevent the test strip 1 from shaking, and the test strip 1 can be attached to the side wall of the first cavity 11 to enhance the chromatography effect, such as As shown in Figure 4. Preferably, the top of the pressure rib 15 is provided with a guide slope 16 for guiding the test strip 1. Under the guidance of the guide slope 16, the test strip 1 can be easily placed at a designated position in the first cavity 11.

Further, as shown in Figure 2, a result observation area 17 is provided on the side wall of the first cavity 11, and a high-gloss convex surface is provided in the result observation area 17. The high-gloss convex surface is an arc convex high-gloss surface design and has a certain convex mirror. Magnification effect facilitates interpretation of results. In this embodiment, the magnification of the high-gloss convex surface for detecting the test strip is 1.3 times. In practical applications, the magnification of the high-gloss convex surface for detecting the test strip is between 1.1 and 1.8, which has a good interpretation effect.

Furthermore, as shown in Figure 3, the first cavity 11 is provided with a ventilation hole 18. After the detection tube is inverted, the test strip 1 can be placed in the same pressure environment as the outside world, which can effectively improve the effect of chromatography detection. The ventilation hole 18 in this embodiment is provided on the bottom surface of the first cavity 11 . During the production process, it can avoid undesirable defects such as product perforation caused by the insertion of the glue punching and bending mold, and can meet the requirements of mold ejection.

Further, as shown in FIG. 4 , the tube body 10 is provided with a second cavity 19 for storing liquid reagents, and a second opening 20 is provided on the top surface of the second cavity 19 . In this embodiment, ribs 21 are provided on the side wall of the second cavity 19 to facilitate elution of the sample on the swab. The scraping effect of the ribs 21 on the swab is used to facilitate elution of the sample on the swab, thereby improving the efficiency of the swab. Sample elution rate.

Further, as shown in FIG. 4 , the bottom of the second cavity 19 is provided with a closing section 22 , the inner diameter of the closing section 22 gradually decreases in the direction from top to bottom, and the bottom of the closing section 22 is provided with an injection molding wavy point 23 . By setting a closing section at the bottom of the second cavity and setting an injection molding wave point at the bottom of the closing section, the bottom of the second cavity can be made thicker to ensure that the mold is filled with glue during the production process.

Further, as shown in Figure 1, the tube cover 30 of this embodiment is provided with a cover groove 31 with an open bottom surface. The notch of the cover groove 31 forms a cover opening 32. The tube cover 30 covers the upper end of the tube body 10 and is in the tube. A third cavity 33 for communicating the first cavity 11 and the second cavity 19 is formed between the cover 30 and the tube body 10 .

As shown in Figure 5, the outer wall of the pipe body 10 in this embodiment is provided with limiting ribs 24 for limiting the pipe cover 30. The limiting ribs 24 divide the pipe body into an upper part 10a and a lower part 10b. , the tube cover 30 covers the upper part of the tube body 10a; the axial length of the upper part of the tube body 10a is less than the depth of the cover groove 31. In this way, after the tube cover 30 covers the upper part of the tube body 10a, the limiting rib 24 Under the action, there is a gap between the bottom of the cover groove 31 and the top surface of the tube body 10, thereby forming the third cavity 33.

Furthermore, a sealing structure is provided between the tube body 10 and the tube cover 30 in this embodiment. The sealing structure of this embodiment includes matching sealing ribs 25 and sealing grooves 34 . Specifically, the sealing structure may be such that the sealing rib 25 is provided on the outer wall of the upper part 10a of the pipe body, and the sealing groove 34 is provided on the side wall of the cover groove 31; the sealing structure may also be such that the sealing groove 34 is provided on the pipe body. On the outer wall of the upper part 10a, sealing ribs 25 are provided on the side walls of the cover groove 31. The sealing ribs 25 of this embodiment are provided on the outer wall of the upper part 10a of the pipe body, and the sealing groove 34 is provided on the side wall of the cover groove 31.

Furthermore, a positioning structure is provided between the tube body 10 and the tube cover 30 in this embodiment. The positioning structure of this embodiment includes positioning protrusions 26 and positioning grooves 35 provided in one-to-one correspondence with the positioning protrusions 26 . Specifically, the positioning structure may be such that the positioning protrusion 26 is provided on the outer wall of the upper part 10a of the pipe body, and the positioning groove 35 is provided on the side wall of the cover groove 31; the positioning structure may also be such that the positioning groove 35 is provided on the pipe body. On the outer wall of the upper part 10a, the positioning protrusion 26 is provided on the side wall of the cover groove 31. The positioning protrusion 26 of this embodiment is provided on the outer wall of the upper part 10a of the pipe body, and the positioning groove 35 is provided on the side wall of the cover groove 31.

The side wall of the cover groove 31 in this embodiment includes an upper side wall section 31a located at the upper part and a lower side wall section 31b located at the lower part. The geometric size of the lower side wall section 31b is larger than the geometric size of the upper side wall section 31a. The positioning protrusion 26 or the positioning recess The groove 35 is provided on the lower section 31b of the side wall, and the sealing rib 25 or the sealing groove 34 is provided Placed on the upper section 31a of the side wall. In this embodiment, the positioning protrusion 26 is provided on the lower section 31b of the side wall, and the sealing rib 25 is provided on the upper section 31a of the side wall.

Further, as shown in FIG. 6 , the bottom of the cover groove 31 is provided with a tube cover protrusion 36 that protrudes downward to reduce the volume of the third cavity 33 to reduce the usage amount of liquid reagent.

The specific implementation of the packaging method of the integrated reaction detection tube of the present application will be described in detail below in conjunction with the specific implementation of the integrated reaction detection tube mentioned above in this embodiment.

The packaging method of the integrated reaction detection tube in this embodiment includes the following steps:

1) After injecting the liquid reagent into the second cavity 19, affix a sealing film on the second opening 20; then place the test strip 1 in the first cavity 11 and extend the first end 2 of the test strip 1. out of the first opening;

2) Place the tube cover 30 on the tube body 10 .

Preferably, before placing the tube cover 30 on the tube body 10, a desiccant is placed in the third cavity 33 to keep the inside of the detection tube dry.

Preferably, after placing the test strip 1 in the first cavity 11 , the fixing plug 13 is installed on the first opening 12 . It plays the role of positioning the first end of the test strip 1, and during the inversion process of the detection tube, it can effectively prevent the splashed liquid reagent from entering the first cavity 11, thereby preventing the splashed liquid reagent from contaminating the NC of the test strip. Membrane 3 can effectively improve the success rate of chromatography detection.

The specific implementation of the method of using the integrated reaction detection tube of the present application will be described in detail below in conjunction with the specific implementation of the integrated reaction detection tube mentioned above in this embodiment.

The method of using the integrated reaction detection tube of this embodiment. Specifically, in the detection tube of this embodiment, the test strip 1 is placed in the first cavity 11, and the liquid reagent is stored in the second cavity 19. A sealing film is attached to the second opening 20 . Specifically, the method of using the integrated reaction detection tube in this embodiment includes the following steps:

Step 1: Remove the tube cover 30 from the tube body 10 and tear off the sealing film on the second opening 20; specifically, when the desiccant is placed in the third cavity 33, remove the tube cover 30 from the tube body 10. After taking it off, first remove the desiccant, and then tear off the sealing film on the second opening 20;

Step 2: Insert the sampling swab into the second cavity 19 to mix the sample to be detected with the liquid reagent, break the sampling swab and leave the broken sampling swab in the second cavity 19;

Step 3: Place the tube cover 30 on the tube body 10;

Step 4: Invert the integrated reaction detection tube of this embodiment, so that the liquid reagent mixed with the sample to be detected contacts the first end 2 of the test strip 1 through the third cavity 33;

Step 5: Observe the test results through the test strip 1.

This embodiment also proposes an application method of an integrated reaction detection tube. The integrated reaction detection tube of this embodiment can be used to detect novel coronavirus, influenza A, influenza B, respiratory syncytial virus or rotavirus. Such as in immunochromatographic detection of adenovirus.

Example 2

As shown in Figure 11, it is a schematic structural diagram of Example 2 of the integrated reaction detection tube of the present application. The integrated reaction detection tube of this embodiment includes a tube body 10 and a tube cover 30. The tube body 10 is provided with a first cavity 11 for placing the test strip 1. The top surface of the first cavity 11 is provided with a test strip 1. The first opening 12 through which the paper strip 1 passes, and a fixed plug 13 (not shown in the figure) is installed on the first opening 12, and the fixed plug 13 is provided with a relief groove that matches the first end 2 of the test strip 1 14. In the integrated reaction detection tube of this embodiment, a first cavity 11 is provided to place the test strip 1, and a fixed plug 13 is provided on the first opening 12 of the first cavity 11. The fixed plug 13 can not only position the test strip 1 The function of the first end of the paper strip 1, and during the inversion process of the detection tube, can effectively prevent the splashed liquid reagent from entering the first cavity 11, thereby preventing the splashed liquid reagent from contaminating the NC film 3 of the test strip, and can Effectively improve the success rate of chromatography detection.

Further, as shown in FIG. 11 , a pressure rib 15 for positioning the second end 4 of the test strip 1 is provided on the side wall of the first cavity 11 . By providing the pressure rib 15, the second end 4 of the test strip 1 can be positioned to prevent the test strip 1 from shaking, and the test strip 1 can be attached to the side wall of the first cavity 11 to enhance the chromatography effect. Preferably, the top of the pressure rib 15 is provided with a guide slope 16 for guiding the test strip 1. Under the guidance of the guide slope 16, the test strip 1 can be easily placed at a designated position in the first cavity 11.

Furthermore, a result observation area 17 is provided on the side wall of the first cavity 11, and a high-gloss convex surface is provided in the result observation area 17. The high-gloss convex surface is an arc convex high-gloss surface design, which has a certain convex mirror magnification effect and facilitates interpretation of the results. . In this embodiment, the magnification of the high-gloss convex surface for detecting the test strip is 1.3 times. In practical applications, the magnification of the high-gloss convex surface for detecting the test strip is between 1.1 and 1.8, which has a good interpretation effect.

Furthermore, the first cavity 11 is provided with a ventilation hole 18. After the detection tube is inverted, the test strip 1 can be placed in the same pressure environment as the outside world, which can effectively improve the effect of chromatography detection. The ventilation hole 18 in this embodiment is provided on the bottom surface of the first cavity 11 . During the production process, it can avoid undesirable defects such as product perforation caused by the insertion of the glue punching and bending mold, and can meet the requirements of mold ejection.

Furthermore, the tube body 10 is provided with a second cavity 19 for storing liquid reagents, and a second opening 20 is provided on the top surface of the second cavity 19 . In this embodiment, the side wall of the second cavity 19 is provided with a convenient The ribs 21 on the swab are used to elute the sample, and the scraping effect of the rib 21 on the swab is used to facilitate elution of the sample on the swab and improve the sample elution rate.

Further, the second cavity 19 includes a lower cavity 19a located at the lower part and a transition section 19b located at the upper part. The second opening 20 is square, and the inner wall of the lower cavity 19a is cylindrical; the transition section 19b is used to connect the second opening 20 and the lower cavity 19a. The cavities 19a are smoothly connected. By providing a transition section to make a smooth transition between the second opening and the lower cavity, liquid can be prevented from hanging at the second opening, which facilitates packaging.

Furthermore, the bottom of the second cavity 19 is provided with a closing section 22 , the inner diameter of the closing section 22 gradually decreases along the direction from top to bottom, and the bottom of the closing section 22 is provided with an injection molding wave point 23 . By setting a closing section at the bottom of the second cavity and setting an injection molding wave point at the bottom of the closing section, the bottom of the second cavity can be made thicker to ensure that the mold is filled with glue during the production process.

Further, as shown in Figure 13, the tube cover 30 of this embodiment is provided with a cover groove 31 with an open bottom surface. The notch of the cover groove 31 forms a cover opening 32. The tube cover 30 covers the upper end of the tube body 10 and is in the tube. A third cavity 33 for communicating the first cavity 11 and the second cavity 19 is formed between the cover 30 and the tube body 10 .

As shown in Figure 12, the outer wall of the pipe body 10 in this embodiment is provided with limiting ribs 24 for limiting the pipe cover 30. The limiting ribs 24 divide the pipe body into an upper part of the pipe body 10a and a lower part of the pipe body 10b. , the tube cover 30 covers the upper part of the tube body 10a; the axial length of the upper part of the tube body 10a is less than the depth of the cover groove 31. In this way, after the tube cover 30 covers the upper part of the tube body 10a, the limiting rib 24 Under the action, there is a gap between the bottom of the cover groove 31 and the top surface of the tube body 10, thereby forming the third cavity 33.

Furthermore, a sealing structure is provided between the pipe body 10 and the pipe cover 30 . The sealing structure of this embodiment adopts a sealing rib 37 provided between the pipe cover 30 and the limiting rib 24 . Specifically, the sealing rib 37 can be arranged on the bottom surface of the tube cover 30 , and the sealing rib can also be arranged on the upper side of the limiting convex rib 24 . The sealing rib 37 of this embodiment can be provided on the bottom surface of the tube cover 30 . In order to ensure sufficient bonding force between the pipe body 10 and the pipe cover 30 to ensure the sealing effect, the upper part 10a of the pipe body in this embodiment is provided with a variable joint for interference fit with the pipe cover 30 near the position of the limiting rib 24. Diameter section 27; the cross-sections taken on the variable-diameter section 27 between any two planes parallel to each other and perpendicular to the axis of the pipe are graphically similar, and the geometric size of the cross-section taken by the plane below is larger than that of the plane above. The obtained geometric dimensions of the cross section can ensure the bonding force between the tube cover 30 and the tube body 10 .

Furthermore, a positioning structure is provided between the tube body 10 and the tube cover 30 in this embodiment. The positioning structure of this embodiment includes positioning protrusions 26 and positioning grooves 35 provided in one-to-one correspondence with the positioning protrusions 26 . Specifically, the positioning structure can be that the positioning protrusion 26 is provided on the outer wall of the upper part of the tube body 10a, and the positioning groove 35 is provided on the side wall of the cover groove 31; the positioning structure can also be that the positioning groove 35 is provided On the outer wall of the upper part 10a of the tube body, the positioning protrusion 26 is provided on the side wall of the cover groove 31. The positioning protrusion 26 of this embodiment is provided on the outer wall of the upper part 10a of the pipe body, and the positioning groove 35 is provided on the side wall of the cover groove 31.

Furthermore, the bottom of the cover groove 31 is provided with a tube cover protrusion 36 that protrudes downward to reduce the volume of the third cavity 33, thereby reducing the usage amount of liquid reagent.

2) Place the tube cover 30 on the tube body 10 .

Step 3: Place the tube cover 30 on the tube body 10;

Step 5: Observe the test results through the test strip 1.

The above-described embodiments are only preferred embodiments to fully illustrate the present application, and the protection scope of the present application is not limited thereto. Equivalent substitutions or transformations made by those skilled in the art on the basis of this application are within the protection scope of this application. The scope of protection of this application shall be determined by the claims.

Claims

An integrated reaction detection tube, including a tube body and a tube cover, characterized in that: the tube body is provided with a first cavity for placing test strips, and the top surface of the first cavity is provided with a A first opening through which the test strip passes, and a fixed plug is installed on the first opening, and a relief groove matching the first end of the test strip is provided on the fixed plug; the first cavity is provided with A ventilation hole is provided, and a result observation area is provided on the side wall of the first cavity.
The integrated reaction detection tube according to claim 1, characterized in that: a pressure rib for positioning the second end of the test strip is provided on the side wall of the first cavity.
The integrated reaction detection tube according to claim 1, wherein the result observation area is provided with a high-gloss convex surface.
The integrated reaction detection tube according to claim 1, characterized in that the ventilation hole is provided on the bottom surface of the first cavity.
The integrated reaction detection tube according to any one of claims 1 to 4, characterized in that: the tube body is provided with a second cavity for storing liquid reagents, and a top surface of the second cavity is provided with The second opening.
The integrated reaction detection tube according to claim 5, wherein the side wall of the second cavity is provided with ribs to facilitate elution of the sample on the swab.
The integrated reaction detection tube according to claim 5, characterized in that: the bottom of the second cavity is provided with a closing section, the inner diameter of the closing section gradually decreases along the direction from top to bottom, and the The bottom of the closing section is provided with injection molding dots.
The integrated reaction detection tube according to claim 5, characterized in that: the second cavity includes a lower cavity located at the lower part and a transition section located at the upper part, the second opening is square, and the inner wall of the lower cavity It is cylindrical; the transition section is used to make a smooth connection between the second opening and the lower cavity.
The integrated reaction detection tube according to claim 5, characterized in that: the tube cover is provided with a cover groove with an open bottom surface, the notch of the cover groove forms a cover opening, and the tube cover covers the tube. The upper end of the body, and a third cavity for communicating the first cavity and the second cavity is formed between the tube cover and the tube body.
The integrated reaction detection tube according to claim 9, characterized in that: the outer wall of the tube body is provided with a limiting convex rib for limiting the tube cover, and the limiting convex rib holds the tube body It is divided into an upper part of the tube body and a lower part of the tube body, and the tube cover covers the upper part of the tube body; the axis of the upper part of the tube body The length is less than the depth of the cover groove.
The integrated reaction detection tube according to claim 10, characterized in that: a sealing structure is provided between the tube body and the tube cover; the sealing structure is provided between the tube cover and the limiting rib. The sealing rib is arranged on the bottom surface of the tube cover, or the sealing rib is arranged on the upper side of the limiting convex rib.
The integrated reaction detection tube according to claim 11, characterized in that: the upper part of the tube body is provided with a reducing section for interference fit with the tube cover at a position close to the limiting rib; any two The cross-sections taken on the variable diameter section of two planes parallel to each other and perpendicular to the axis of the pipe are similar in shape, and the geometric size of the cross-section taken by the plane below is larger than the cross-section taken by the plane above. geometric dimensions.
The integrated reaction detection tube according to claim 9, characterized in that: a sealing structure is provided between the tube body and the tube cover; the sealing structure includes mutually matching sealing ribs and sealing grooves; the sealing The ribs are provided on the outer wall of the upper part of the pipe body, and the sealing groove is provided on the side wall of the cover groove; or, the sealing groove is provided on the outer wall of the upper part of the pipe body, and the sealing groove is provided on the outer wall of the upper part of the pipe body. The ribs are arranged on the side walls of the cover groove.
The integrated reaction detection tube according to claim 9, characterized in that: a positioning structure is provided between the tube body and the tube cover; the positioning structure includes a positioning protrusion and a one-to-one correspondence with the positioning protrusion. The positioning groove; the positioning protrusion is provided on the outer wall of the upper part of the pipe body, the positioning groove is provided on the side wall of the cover groove; or the positioning groove is provided on the pipe body On the outer wall of the upper part, the positioning protrusion is provided on the side wall of the cover groove.
The integrated reaction detection tube according to claim 14, characterized in that: a sealing structure is provided between the tube body and the tube cover, and the sealing structure includes mutually matching sealing ribs and sealing grooves;

The side wall of the cover groove includes an upper section of the side wall located at the upper part and a lower section of the side wall located at the lower part. The geometric size of the lower section of the side wall is larger than the geometric size of the upper section of the side wall. The positioning protrusion or positioning groove is provided On the lower section of the side wall, the sealing rib or sealing groove is provided on the upper section of the side wall.
The integrated reaction detection tube according to claim 9, wherein the bottom of the cover groove is provided with a tube cover protrusion that protrudes downward to reduce the volume of the third cavity.
A packaging method for the integrated reaction detection tube according to any one of claims 9-16, characterized in that it includes the following steps:

1) After injecting the liquid reagent into the second cavity, affix a sealing film on the second opening; and then A test strip is placed in the first cavity and the first end of the test strip extends out of the first opening;

2) Place the tube cap on the tube body.
The packaging method of the integrated reaction detection tube according to claim 17, characterized in that: in the step 2), before the tube cover is placed on the tube body, a dryer is placed in the third cavity. agent.
The packaging method of the integrated reaction detection tube according to claim 17, characterized in that in step 1), after placing the test strip in the first cavity, a fixing plug is installed on the first opening.
A method of using the integrated reaction detection tube according to any one of claims 9 to 16, characterized in that: a test strip is placed in the first cavity, a liquid reagent is stored in the second cavity, and A sealing film is attached to the second opening; the steps include:

Step 1: Remove the tube cover from the tube body and tear off the sealing film on the second opening;

Step 2: Insert the sampling swab into the second cavity, mix the sample to be detected with the liquid reagent, break the sampling swab and leave the broken sampling swab in the second cavity;

Step 3: Place the tube cap on the tube body;

Step 4: Invert the integrated reaction detection tube so that the liquid reagent mixed with the sample to be detected passes through the third chamber and contacts the first end of the test strip;

Step 5: Observe the test results through the test strip.
The method of using the integrated reaction detection tube according to claim 20, characterized in that: in the step one, when the desiccant is placed in the third chamber, after the tube cover is removed from the tube body, first Remove the desiccant and peel off the sealing film on the second opening.
Application of an integrated reaction detection tube according to any one of claims 9 to 16 in the immunochromatographic detection of novel coronavirus, influenza A, influenza B, respiratory syncytial virus or rotadenovirus .