CN105802834B - Closed component - Google Patents
Closed component Download PDFInfo
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- CN105802834B CN105802834B CN201610345309.3A CN201610345309A CN105802834B CN 105802834 B CN105802834 B CN 105802834B CN 201610345309 A CN201610345309 A CN 201610345309A CN 105802834 B CN105802834 B CN 105802834B
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- China
- Prior art keywords
- port
- cap
- snap cap
- stop part
- annular
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D51/00—Closures not otherwise provided for
- B65D51/002—Closures to be pierced by an extracting-device for the contents and fixed on the container by separate retaining means
Abstract
The present invention relates to a kind of closed components.The closed component includes：Snap cap, the annular sidewall that snap cap has the top for limiting opening and extends from top, wherein, the side wall of snap cap includes being configured to engage with the snap cap engagement feature of the port of cell culture apparatus to annular protruding member to annular protruding member；Stop part, stop part include distal portions, and distal portions include the distal extension for limiting inner cavity, and distal portions further include the annular flange extended from stop part, wherein the bottom surface of snap cap is configured to compress annular flange；Wherein, the distal extension of stop part is configured to extend through the opening in the top of snap cap.
Description
The application is that international filing date is on November 18th, 2011, international application no PCT/US2011/061298, enters
National Phase in China application No. is the 201180055752.2, patents of invention of entitled " closed component of cell culture apparatus "
The divisional application of application.
Cross reference to related applications
The U.S.Provisional Serial the 61/th that the application requires to submit on November 22nd, 2010 according to 35U.S.C. § 119
415,970 benefit of priority, this application it is whole using in a manner of as according to and be included in this paper.
Technical field
The present invention relates to cell culture apparatus, and relate more specifically to port and the closed component of this device.
Background technology
Cell culture apparatus has the port that cell, culture medium or other fluids are introduced to cell culture chamber.In general,
Cap be used for when fluid or cell are not introduced into sealed port to prevent contamination of cells culturing room.In many situations, cap has spiral shell
External screw thread is turning round cap onto port on line and engageable port.The advantages of this torsion cap, which is them, can easily be removed to touch
And Cytology Lab port.In the research or development of early stage, it usually needs repeatedly turn round upper cap and turn round lower cap with from culturing room
It obtains sample or reagent or fluid is introduced into culturing room.
But in the later stage developed or confirmed, for the approval of supervision department, it usually needs or culturing room is required to close
It closes or seals.Although turning round cap can be used for this purpose, it is often difficult to determine that cap is suitably sealed；Such as cannot tilt, or really
Determining cap will not unexpectedly be removed.
Correspondingly, for the research or development of early stage and the exploitation in later stage or confirmation stage, through frequently with different cultures
Device.Early stage is using the device that can easily remove cap, and the later stage is using the device for less easily removing cap.But at these
It is typically undesired to switch culture apparatus in phase process, because the result obtained in the first culture apparatus may not be able to be
It is repeated and observes in second different culture apparatus.
Invention content
The present invention particularly depicts the cell culture apparatus with the port for being configured to engagement torsion cap or snap cap.Very much
In embodiment, port and torsion cap are configured to allow for cap readily to remove and attach relative to port.On the contrary, snap cap and end
Outlet structure is at making when the snap cap and port are fully engaged, if any, the snap cap cannot pass through independence
Manpower and easily removed from port.By by port configuration at engaging removable cap and not removable cap, it is identical
Culture apparatus can be used for wherein needing the early stage research of opening and closing system and wherein may need or require for good and all
The purpose of the tightened up confirmation of closing system.
In various embodiments described herein, cell culture apparatus includes cell culture chamber and port, and port has annular side
Wall, the annular sidewall limit the opening being connected to cell culture chamber.There is (i) to be configured to and turn round the interior of cap for the annular sidewall of port
The external screw thread of screw-thread fit and (ii) are configured to protrude snap cap engagement feature with the annular for turning round cap cooperation.The connected structure has
There is top surface and bottom surface, the structure which is configured to engagement snap cap is drawn to resist snap cap from port
Fall.The bottom surface of connected structure is extended with the angle less than 110 degree from the side wall of port.
In multiple embodiments as described herein, the closed component of cell culture apparatus includes port and snap cap.The end
There is mouth annular sidewall, the annular sidewall to limit the opening being connected to the cell culture chamber of cell culture apparatus.The side wall includes
(i) snap cap engagement feature is protruded with the external screw thread of the screw-internal thread fit of torsion cap and (ii) annular.The snap cap has top
Portion and the annular sidewall extended from the top.The side wall of snap cap has to annular protruding member, this is prominent to annular
Component is configured to engage with the prominent snap cap engagement feature of the annular of port so that when fully engaged, snap cap is not allowed
Easily removed from port by individual manpower.The prominent snap cap engagement feature of annular of the port has top surface and bottom
Portion, wherein the bottom surface of connected structure is extended with the angle less than 110 degree from the side wall of port.Snap cap to annular
Protruding member has top surface and bottom surface, wherein snap cap is configured to the top surface of annular protruding member when drawing
The bottom surface of connected structure when power is applied to cap with port engages.The top surface to annular protruding member of snap cap can
To be extended from the surface of side wall with the angle less than 110 degree.
Closed component may also include stop part, which has proximal part and distal portions, proximal part construction
At the opening receiving by port, and the distal portions are configured to be maintained at the outside of port.Distal portions have flange, the flange
With the outer diameter bigger in the internal diameter of opening than the side wall of port.The bottom surface at the top of snap cap is configured to work as snap cap
The flange of stop part is compressed when being fully engaged with port against the top of port.In some embodiments, this can also force stop part
Against the side wall of port.
Component, device, object and method as described herein can provide one or much better than previous cell culture system
A advantage.For example, can study and open to be used for early stage using the Single cell culture device with the cap that can easily remove
It puts, and approves acquisition really and for these using the same apparatus with the different caps that can not easily remove to be used for the later stage
The associated potential abnormal results of use of the different culture apparatus of purpose.For those skilled in the art, this paper institutes are being read
After the disclosure of presentation, these and other of each embodiment of component as described herein, device, object and method will readily appreciate that
Advantage.
Description of the drawings
Fig. 1 is the exemplary diagrammatic top view of cell culture apparatus.
Fig. 2 is the schematic cross sectional views of the slave line 2-2 interceptions of the embodiment shown in FIG. 1 in device.
Fig. 3 is the diagrammatic side view of the port of cell culture apparatus.
Fig. 4 is the schematic cross sectional views of the slave line 4-4 interceptions of the embodiment shown in FIG. 1 in the port of device.
Fig. 5 is the diagrammatic side view of the port of cell culture apparatus.
Fig. 6 A and 6B are the signals of the embodiment of the stop part for the port that can be at least partially inserted into cell culture apparatus
Property side view and vertical view.
Fig. 7 be stop part and by stop part inner cavity receive pipeline embodiment schematic cross sectional views.
Fig. 8 is structured to the schematic side elevation of the embodiment of the snap cap of the port of zygoneure culture apparatus.
Fig. 9 is the schematic plan of the embodiment of snap cap shown in Fig. 8.
Figure 10 is the schematic cross sectional views of the embodiment of cap shown in Fig. 8.
Figure 11 is the signal of the closed component for the cell culture apparatus for showing port, stop part, snap cap and pipeline
Property side view.
Figure 12 is the cooperation connected structure of the snap cap and port that show to be completely placed on port and engaged with port
Schematic partial enlarged view.
Explanatory view presented herein does not need proportional.The same reference numerals instruction used in attached drawing is identical
Component, step etc..It is understood, however, that it is another attached to indicate that the component in given attached drawing is not limiting as using reference numeral
The component of same reference numerals is marked in figure.In addition, indicating that each component is not intended to instruction not isolabeling using different reference numerals
Each component cannot make it is same or analogous.
Specific implementation mode
In the following detailed description, with reference to formed this part and device, system and side are wherein illustratively shown
The attached drawing of multiple specific embodiments of method.It should be appreciated that also contemplating for and other embodiments can be made without departing from the present invention's
Range or spirit.It therefore, should not be described in detail below to understand with the meaning of restriction.
All scientific and technical terms used herein have the meaning usually used in this field, unless referring in particular to
Go out.Definition provided in this article is easy for the understanding of some terms frequently used herein and is not intended to limit the present invention's
Range.
As used in the specification and the appended claims, the "a", "an" and "the" packet of singulative
The embodiment with plural reference is included, unless the context clearly indicates otherwise.
As used in this specification and the appended claims, term "or" be generally adopted as in its meaning include
"and/or", unless the context clearly indicates otherwise
As it is used herein, " having ", " containing ", " composition ", " composition ", "include", "comprise" etc. are open at its
It is used in meaning, and generally means that " including, but are not limited to ".It will be understood that term " by ... constitute " and " substantially by ... constitute "
It is contained in term " comprising " etc..For example, the closed component including port, cap and stop part of cell culture apparatus can by or it is basic
On be made of port, cap and stop part.
" substantially by ... constitute ", when it is related to component, object, system, device or method, means the component, object
Part, system, device or method only include each component, object, system, each component of device or method or the step being previously mentioned, with
And do not influence each component, object, system, basic and novel characteristics other components of device or method or step substantially optionally
Suddenly.
Any direction cited herein, such as " top ", " bottom ", "left", "right", "upper", "lower", " top ", " under
Side " and other directions and orientation, are herein defined as in order to which refer to the attached drawing understands and describes, it is no intended to limit actual device
Or the use of system or the device or system.Many devices, object or system described herein can be in multiple directions and fixed
It is used up.
The present invention particularly depicts the cell culture apparatus with the port for being configured to engagement torsion cap or snap cap.Very much
In embodiment, port and torsion cap are configured to allow for cap to be easy to remove and attach relative to port, and snap cap and port configuration
At making when the snap cap and port are fully engaged, if any, the snap cap cannot pass through independent manpower
And it is easily removed from port.By by port configuration at engaging removable cap and relatively not removable cap, identical training
Foster device can be used for wherein needing the early stage research of opening and closing system and may wherein need or require for good and all to close
Close the purpose of the tightened up confirmation of system.
Closed component described herein can be used in any suitable cell culture system.For example, tank, flask, bottle,
Disk, beaker, test tube, bag, perfusion compartment, bioreactor, Corning IncorporatedCultivate chamber device, and fermentation
Tank can be easily adapted to include closed component or its component.
By way of example and with reference to figure 1, be shown showing for the cell culture apparatus 10 containing port 100 as described herein
The schematic plan of example.Shown cell culture apparatus 10 have top surface 15 and from the top surface extend two ports
100.Although showing two ports, it will be appreciated that cell culture apparatus 10 may include any appropriate number of port 100.To also
Although what is understood is that port 100 is shown as extending from the top surface 15 of device 100, one or more ports 100 can be positioned on dress
It sets at 15 any suitable position to provide the access of the interior chamber of device.
Referring now to Fig. 2, the schematic cross sectional views of the line 2-2 interceptions of the embodiment of the cell culture apparatus along Fig. 1 are shown.Institute
The device 10 shown includes the cell culture chamber 20 limited by roof 12, bottom wall 14 and side wall 16.Shown in embodiment, training
The roof 12 for supporting room 20 also serves as the top of the device.(see, for example, Fig. 1) is connected to room 20 to carry for one or more ports 100
It is supplied to the access of the room.Certainly, device 10 shown in Fig. 2 is very simple device, it will be appreciated that, more complicated device,
The room of introducing fluid such as with multiple cell culture chambers, tracheae room, or adjacent to cell culture chamber, can be modified to include
Port as described herein or closed component.In any situation, port as described herein provide to one of cell culture apparatus or
The access of multiple rooms.
Referring now to Fig. 3-4, the embodiment for being configured to the cell culture apparatus port 100 that cap or snap cap are turned round in engagement is shown
Schematic side elevational and sectional view.There is annular sidewall 130, annular sidewall 130 to have the interior spiral shell for being configured to and turning round cap for port 100
The external screw thread 110 that line engages and coordinates.Annular snap cap engagement feature 120 outstanding extend from side wall 130 and be configured to
Snap cap engages and coordinates.In embodiment shown in Fig. 3-4, snap cap engagement feature 120 is than screw thread 110 closer to cell
The surface 15 of culture apparatus positions.In addition, the maximum outside diameter (od2) of annular snap cap engagement feature 120 outstanding is more than outer
The maximum outside diameter (od1) of screw thread 110.This allow snap cap cooperation joint element 110 top row of screw thread into without with card
The advance thread interference for entering formula cap, as further discussed below with reference to Figure 10.
In embodiment shown in Fig. 4, the annular sidewall 130 of port 100 limits inner cavity 140, the outside of inner cavity 140 and port
It is connected to one or more rooms of culture apparatus.Shown in inner cavity 140 it is tapered so that its at the outward opening closer to port
With larger internal diameter (id1) closer at cell culture apparatus with smaller internal diameter (id2).When need use backstop
When part, this taper can provide the improved sealing engagement between the inner surface and stop part of the side wall 130 of port 100.Certainly,
For the port not with this conical inner side wall, many suitable stop parts can be used.
Referring now to Fig. 5, the embodiment for being configured to the cell culture apparatus port 100 that cap or snap cap are turned round in engagement is shown
Schematic side elevation.Shown in embodiment, external screw thread 110 is configured to engage and coordinate with the internal thread for turning round cap, than being caught in
Formula cap connected structure 120 closer to cell culture apparatus surface position, snap cap engagement feature 120 be configured to it is snap-in
Cap engages and coordinates.In the embodiment, the maximum outside diameter (od2) of annular snap cap engagement feature 120 outstanding is less than outer spiral shell
The maximum outside diameter (od1) of line 110.This allows the internal thread for turning round cap, is configured to coordinate and engage with external screw thread 110, be caught in
120 top of formula connected structure is advanced, and traveling of the structure 120 not with torsion cap around port 100 is interfered.Certainly, screw thread 110 and card
Entering formula cap connected structure 120 can be placed in any suitable position relative to each other, as long as snap cap can be suitably
Connected structure 120 and turn round cap can suitably engage screw thread 110.
Line shows the embodiment for the stop part 200 that can be used together with closed component as described herein with reference to figure 6A, 6B
Schematic side elevation and vertical view.Stop part 200 is configured to be accepted in and be sealingly engaged to the port of cell culture apparatus.
Shown in stop part 200 have be configured to by port inner cavity receive proximal part 210 and be configured to be maintained at outside port
Distal portions 220.Proximal part 210 may include from the outwardly extending one or more annular rings of the ontology 240 of stop part 200
250 (showing two).Annular ring 250 is configured to sealingly engage the inner surface of the annular sidewall of port.
Shown in the distal portions 220 of stop part 200 include the annular flange radially extended from the ontology 240 of stop part
230.The bottom surface of flange 230 is configured to sealingly engage the top surface of port.Correspondingly, the outer diameter of flange 230 is more than port
Internal diameter of the annular sidewall at the outward opening of port.Such as snap cap (for example, as further described below with reference to Figure 11)
The bottom surface of cap can be configured to the top surface press flange when cap and port are fully engaged against port.
The distal portions 220 of stop part 200 shown in embodiment shown in Fig. 6 A include distal extension 260, distal end
Extension 260 limits inner cavity 270 (referring to Fig. 6 B), and inner cavity 270 is configured to seal when stop part 200 is hermetically engaged with port
Pipeline for gas or fluid to be introduced to culture apparatus by port.Any suitable pipeline, such as glass tube, metal tube or
Plastic tube can be used in this purpose.
Referring now to Fig. 7, show that the stop part 200 shown in Fig. 6 A and 6B is the schematic cross sectional views of embodiment.In Fig. 7,
Pipeline 300 is shown as being inserted into the inner cavity 270 limited by the distal extension 260 of stop part 200 (referring to Fig. 6 B).Shown in it is real
Apply in example, the inside of stop part 200 formed charge into the annular shoulder of inner cavity with prevent pipeline 300 further towards being inserted into backstop
Part 200.Pipeline 300 can be inserted into stop part 200 by the terminal user of cell culture apparatus or can be stopped by manufacturer's pre-inserted
Block piece 200.Frictional force can keep pipeline 300 or pipeline glued, welding or otherwise can be labelled to backstop relative to stop part 200
Part 200.In some embodiments, pipeline 300 is molded as a part for stop part 200, reduce stop part 200 and pipeline 300 it
Between potential leakage point.In embodiment shown in Fig. 7, the interior shape of stop part 300 is designed to reduce fluid in stop part 300
It is stayed with region near port 100, allows completely or nearly complete fluid removal.
Referring now to Fig. 8-10, the schematic side elevation, vertical view and sectional view of the embodiment of snap cap 400 are shown.Institute
There is the snap cap shown the top 405 for limiting opening 410, the distal extension of stop part may pass through the opening 410 protrusion.Cap
400 include the annular sidewall 415 or skirt extended from top 405.Contain in the end at the top 405 far from cap in side wall 415
There are one or multiple gaps 430.Extension 425 is formed between adjacent gap 430.Extension 425 be elasticity and it is radial to
It can deviate outside.Annular protruding member 440 extends internally from side wall 415, in the situation shown in, from side wall extension 425 to
Interior extension.Such as will be discussed in detail below in relation to Figure 11-12, to the snap-in of annular component 440 outstanding and port
Cap connected structure coordinates.Preferably, when snap cap 400 and port are fully engaged, cap 400 cannot pass through independent manpower quilt
It removes.That is, snap cap 400 is used as providing the permanent cap of the cell culture apparatus of permanent seal.
In various embodiments, snap cap and port configuration at prevent snap cap from by being less than 50,75,100,
125 or 150 pounds of pulling force and removed from port.In some embodiments, snap cap and port configuration are at making snap cap not
It can be pulled off from port without destroying snap cap or port or part of it.
Snap cap 400 may include flange 420, and in an illustrated embodiment, flange 420 extends together with top 405, can
For providing a surface, the surface drag cap 400 can be resisted to determine whether cap is fully engaged with port (that is, suitably
It is caught in place around port).In addition, snap cap 400 may include that annular positioning groove 435, locating slot 435 are dashed forward with to annular
Go out the identical outside (side wall extension 425 in the situation) for surrounding side wall 415 at top 405 of component 440 to extend.Positioning
Slot 435 or other suitable structures, are combined with gap 430, can be used for visually verifying to annular protruding member 440 whether with
The snap cap engagement feature of port suitably engages and (is fully engaged and does not tilt for example, verifying snap cap).
The internal diameter (id) bigger than the externally threaded outer diameter of port is limited to annular component 440 outstanding shown in Figure 10,
To which cap 400 can external screw thread not be interfered with the traveling of cap in turn in port top row.(not shown) in some embodiments, it is snap-in
Cap may also include the internal thread for being configured to coordinate with the external screw thread of port, to which snap cap can be rotated being caught in until cap
Component and port engagement.But in some situations, it may be necessary to this internal whorl is omitted, because for this twist mechanism,
Tilt often problem.
Referring now to figure 11, the schematic cross sectional views of the closed component of cell culture apparatus, identical reference numeral table are shown
Show and about the same parts described shown in Fig. 1-10.Shown in component include port (i) 100, which is attached to,
It is formed integrally or otherwise be labelled to cell culture apparatus 10, (ii) stop part 200, the stop part 200 be received in and with end
It engages to the bore seal of mouth 400, (iii) pipeline 300, the pipeline 300 is received in, and with the bore seal of stop part 200
It engages, and the cap 400 that (iv) is fully engaged with port 100.When cap 400 and port 100 are fully engaged, the top 405 of cap
Bottom surface be engaged in and against the top surface of port 100 press stop part 200 flange 230 to form Fluid Sealing and also force
Side wall of the stop part against port.May need sealed port 100 without provide via pipeline 300 to port access it is each
In embodiment, the top 405 of cap can be solid (imperforation), and washer or other compressible materials can substitute stop part
Flange to provide Fluid Sealing.
As shown in figure 11, the prominent snap cap engagement feature 120 of annular of port 100 and cap to annular structure outstanding
Part 440 coordinates to prevent cap 400 from being pulled off port 100.
Shown in Figure 12 port the prominent snap cap engagement feature 120 of annular and cap to annular protruding member 440
Embodiment other details.In an illustrated embodiment, the prominent snap cap engagement feature 120 of the annular of port has from end
The bottom surface 122 and top surface 124 that the side wall 130 of mouth extends.For the purposes of the present invention, top surface 124 is to be substantially dorsal to
The surface on the surface (such as surface 15 of Fig. 4) of cell culture apparatus, and bottom surface 122 is to be substantially facing cell culture apparatus
Surface surface.
Snap cap has the bottom surface 442 extended from the side wall 425 of cap and top table to annular component 440 outstanding
Face 444.For the purposes of the present invention, bottom surface 442 is the opening for being configured to advance above port for being substantially facing cap
Surface, and top surface 444 is the surface for the opening for being substantially dorsal to cap.
Still referring to FIG. 12, the bottom surface 112 of the prominent snap cap engagement feature of the annular of port 120 is with angle (α1) from end
Mouth side wall 130 extends.Snap cap to annular component 440 outstanding with about α1Angle from cap side wall 425 extend.In order to
Preventing between annular protruding member 440 and the annular prominent snap cap engagement feature 120 of port for snap cap is provided
The interaction that only cap is pulled out from port, α190 ± 20 degree (i.e. 70 and 110 degree between, between such as 100 and 80 degree).
Snap cap is engaged to the bottom surface 442 of annular protruding member 440 snap cap prominent with the annular of port
The top surface 124 of structure 120 coordinates in order to which cap surrounds the placement of port and is fully engaged.It is pressed downward with port when cap is opposite
When, the annular prominent snap cap engagement feature that port is engaged to the inclined bottom face 442 of annular protruding member 440 of cap
120 top surface 124, causes side wall extension 425 radially outward to deviate.Once cap is pushed away enough distances, side relative to port
Wall extension 425, which restores it, to be loosened the annular protruding member 440 of construction and cap and not to contact the prominent snap cap of annular of port and connect
Close structure 120 (being fully engaged in construction that is, being in shown in Figure 12).It is connect completely to help cap to be moved into relative to port
Close position, the bottom surface 442 to annular protruding member 440 of cap is from cap side wall 415 with the angle [alpha] of 135 ± 15 degree of angle2
(i.e. between 120 and 150 degree, such as between 130 and 140 degree) extends from cap side wall 415.The annular of port protrudes snap-in
The top surface 124 of cap connected structure 120 is with about α2Angle from port side wall 130 extend.
Certainly, the cooperation of cap and port be caught in the top surface and bottom surface of structure can structure in any other suitable way
It causes to be convenient for placement and engagement of the cap relative to port.For example, each surface can be rounded corner, polishing or coating in order to put
It sets.By another exemplary mode, each surface can be coarse, containing latch and gripper or other fit structures in order to complete
Full engagement (and thus prevent or cap is forbidden to be removed from port).
Port as described herein can be made of any suitable material, hard plastic material, glass, metal etc..
The example of suitable plastic material includes high density polyethylene (HDPE) (HDPE), polypropylene, makrolon, polystyrene etc..Preferably,
Port is formed by bio-compatible material.In some embodiments, port by with the same or analogous material of the shell of cell culture apparatus
Material is formed.The port can be a part of integrally moulded with the shell of cell culture apparatus, can weld, be bonded or otherwise
It is labelled to cell culture apparatus.
Stop part as described herein can be made of any suitable material, such as rubber material.Suitable rubber material
Example include butyl rubber, silicon rubber etc..Preferably, stop part is formed by bio-compatible material.Stop part can be by suitable
When technique formed, such as mould.In some embodiments, the annular ring or flange of stop part are by suitable rubber material system
At, and ontology is formed by another material, such as hard plastic material.
Cap as described herein can be made of any suitable material, hard plastic material, metal etc..Suitable modeling
Expect that the example of material includes high density polyethylene (HDPE) (HDPE), polypropylene, makrolon etc..Preferably, cap is by bio-compatible material
It is formed.In some embodiments, cap with the same or analogous material in port by forming.Cap can be formed by technique appropriate,
Such as mould.
Various aspects of the present invention describe object, component and method.
In first aspect, cell culture apparatus is described.The cell culture apparatus includes cell culture chamber and port.Port
With annular sidewall, annular sidewall limits the opening being connected to cell culture chamber.Side wall includes the interior spiral shell that (i) is configured to and turns round cap
The external screw thread and the prominent snap cap engagement feature of (ii) annular of line cooperation.The connected structure has top surface and bottom surface, should
The structure that connected structure is configured to engagement snap cap is pulled off with resisting snap cap from port.The bottom surface of connected structure with
Angle less than 110 degree extends from side wall.
Second aspect is the cell culture apparatus of first aspect, wherein the bottom surface of connected structure with 105 degree and 75 degree it
Between angle from side wall extend.
The third aspect is the cell culture apparatus of first aspect, wherein the bottom surface of connected structure with 100 degree and 80 degree it
Between angle from side wall extend.
Fourth aspect is the cell culture apparatus of either side in the first to three aspect, wherein the top surface of connected structure
Extended from side wall with the angle between 120 degree and 150 degree.
5th aspect be first to either side in the third aspect cell culture apparatus, wherein the top table of connected structure
Face is extended with the angle between 130 degree and 140 degree from side wall.
6th aspect is the cell culture apparatus of either side in the first to the 5th aspect, wherein annular protrusion is snap-in
Cap connected structure limits the maximum outside diameter bigger than the maximum outside diameter limited by external screw thread.
7th aspect is the cell culture apparatus of the 6th aspect, wherein the prominent snap cap engagement feature of annular is along side wall
It is located in than external screw thread closer at the position of cell culture chamber.
Eighth aspect is the closed component of cell culture apparatus.The closed component includes port and snap cap.The port
With annular sidewall, annular sidewall limits the opening that is connected to the cell culture chamber of cell culture apparatus, wherein side wall includes
(i) external screw thread and the prominent snap cap engagement feature of (ii) annular with the screw-internal thread fit for turning round cap are configured to.The snap cap
The annular sidewall extended with top and from top, wherein the side wall of snap cap includes to annular protruding member, this is inwardly
Annular protruding member is configured to engage with the prominent snap cap engagement feature of the annular of port so that when fully engaged, is caught in
Formula cap is easily detected by independent manpower and is removed from port.
9th aspect is the closed component of eighth aspect, wherein snap cap and port configuration are at making snap cap not
It can be removed from port by pulling force less than 75 pounds.
Tenth aspect is the closed component of eighth aspect, wherein snap cap is configured so that snap cap cannot pass through
Drawn on cap on the axis direction along the opening of port the annular to be removed from port without destroying port protrude it is snap-in
Cap connected structure or snap cap to annular protruding member.
Tenth one side is the closed component of either side in 8-10 aspects, wherein the annular of port protrudes snap-in
Cap connected structure has top surface and bottom surface, wherein the bottom surface of connected structure is with the angle less than 110 degree from the side of port
Wall extends, and wherein, and snap cap has top surface and bottom surface to annular protruding member, wherein snap cap
The bottom surface that the connected structure when pulling force is applied to cap with port is configured to the top surface of annular protruding member engages, with
And wherein, the top surface to annular protruding member of snap cap is extended with the angle less than 110 degree from the surface of side wall.
12nd aspect be the tenth one side closed component, wherein the bottom surface of the connected structure of port with 100 degree with
Angle between 80 degree extends from the side wall of port, and wherein, the top surface to annular protruding member of snap cap with
Angle between 100 degree and 80 degree extends from the surface of side wall.
13rd aspect is the closed component of either side in 8-11 aspects, wherein the top surface of connected structure is with 120
Spend the angle between 150 degree from side wall extension, and wherein, the bottom surface to annular protruding member of snap cap with
Angle between 120 degree and 150 degree extends from the surface of side wall.
Fourteenth aspect is the closed component of either side in 8-13 aspects, wherein the annular of port protrudes snap-in
Cap connected structure limits the maximum outside diameter bigger than the maximum outside diameter limited by the external screw thread of port.
15th aspect is the closed component of fourteenth aspect, wherein the prominent snap cap engagement feature of annular is along side wall
It is located in than external screw thread closer at the position of cell culture chamber.
16th aspect be in terms of 8-15 in either side closed component, wherein further include with proximal part and
The stop part of distal portions, the proximal part are configured to be received by the opening of port, which is configured to be maintained at port
It is external and include with than the side wall of port the big outer diameter of the internal diameter of opening flange, wherein the top of snap cap
Bottom surface is configured to the flange that the top when snap cap is fully engaged with port against port compresses stop part.Some are implemented
In example, this also can force stop part against the side wall of port.
17th aspect is the closed component of the 16th aspect, wherein stop part includes being configured to compressiblly abutting end
The annular ring of the inner surface of the side wall of mouth.
18th aspect is the closed component of the 16th aspect, wherein stop part includes being configured to compressiblly abutting end
Multiple annular rings of the inner surface of the side wall of mouth.
19th aspect is the closed component of either side in 8-16 aspects, wherein side wall is included in far from top
Two or more gaps at part, wherein the distal portions of side wall form extension between two gaps, the extension
It is elasticity and can deviates outward.
20th aspect be the 19th aspect closed component, wherein snap cap to annular protruding member from this
Extension extends.
20th one side is the cell culture system for including the closed component of either side in 8-20 aspects.
Each embodiment of the closed component of cell culture apparatus is disclosed as a result,.It will be apparent to one skilled in the art that this
Cell culture apparatus and method described in text can be implemented by those embodiments except disclosed.The disclosed embodiments
For illustration purposes rather than limit purpose.
Claims (7)
1. a kind of closed component, including：
Snap cap, the annular sidewall that the snap cap has the top for limiting opening and extends from the top, wherein institute
The side wall for stating snap cap include to annular protruding member, it is described to be configured to annular protruding member and cell culture apparatus
Port snap cap engagement feature engagement；
Stop part, the stop part include distal portions, and the distal portions include the distal extension for limiting inner cavity, described remote
End part further includes the annular flange extended from the stop part, wherein the bottom surface of the snap cap is configured to compress institute
State annular flange；
Wherein, the distal extension of the stop part is configured to extend through the opening in the top of the snap cap.
2. closed component as described in claim 1, which is characterized in that the snap cap further includes internal thread, the interior spiral shell
Line is configured to match with the external screw thread of the port of the cell culture apparatus.
3. closed component as described in claim 1, which is characterized in that the side wall of the snap cap further include it is multiple can be radial
The extension deviated outward, the extension are separated by one or more gaps.
4. closed component as described in claim 1, which is characterized in that the distal extension of the stop part is configured to hermetically
Receive pipeline.
5. closed component as claimed in claim 4, which is characterized in that the stop part further includes annular shoulder, the annular
Shoulder charges into the interior intracavitary and is inserted into the inner cavity to limit the pipeline.
6. the closed component as described in any one of claim 4-5, which is characterized in that the pipeline is labelled to the stop part.
7. the closed component as described in any one of claim 4-5, which is characterized in that frictional force is protected relative to the stop part
Hold the pipeline.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41597010P | 2010-11-22 | 2010-11-22 | |
| US61/415,970 | 2010-11-22 | ||
| CN201180055752.2A CN103221134B (en) | 2010-11-22 | 2011-11-18 | The closed component of cell culture system |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180055752.2A Division CN103221134B (en) | 2010-11-22 | 2011-11-18 | The closed component of cell culture system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105802834A CN105802834A (en) | 2016-07-27 |
| CN105802834B true CN105802834B (en) | 2018-07-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180055752.2A Active CN103221134B (en) | 2010-11-22 | 2011-11-18 | The closed component of cell culture system |
| CN201610345309.3A Active CN105802834B (en) | 2010-11-22 | 2011-11-18 | Closed component |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180055752.2A Active CN103221134B (en) | 2010-11-22 | 2011-11-18 | The closed component of cell culture system |
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| US (2) | US10100273B2 (en) |
| EP (2) | EP3210670B1 (en) |
| JP (2) | JP5961178B2 (en) |
| CN (2) | CN103221134B (en) |
| DK (1) | DK3210670T3 (en) |
| ES (1) | ES2788500T3 (en) |
| PL (1) | PL2643088T3 (en) |
| PT (1) | PT2643088T (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11578296B2 (en) | 2016-11-30 | 2023-02-14 | Corning Incorporated | Reinforced barbed closure |
| EP3501658B1 (en) * | 2019-03-19 | 2021-04-21 | Ace Medical Technology Co., Ltd. | Device for sorting bio-particles using a force generated from light-induced dielectrophoresis and operating method thereof |
| WO2022190261A1 (en) * | 2021-03-10 | 2022-09-15 | 株式会社サンプラテック | Cell culture vessel |
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- 2011-11-17 US US13/298,679 patent/US10100273B2/en active Active
- 2011-11-18 DK DK17166452.7T patent/DK3210670T3/en active
- 2011-11-18 PT PT117968537T patent/PT2643088T/en unknown
- 2011-11-18 PL PL11796853T patent/PL2643088T3/en unknown
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- 2011-11-18 EP EP17166452.7A patent/EP3210670B1/en active Active
- 2011-11-18 CN CN201610345309.3A patent/CN105802834B/en active Active
- 2011-11-18 JP JP2013540976A patent/JP5961178B2/en active Active
- 2011-11-18 EP EP11796853.7A patent/EP2643088B1/en active Active
- 2011-11-18 WO PCT/US2011/061298 patent/WO2012071249A1/en active Application Filing
- 2011-11-18 ES ES11796853T patent/ES2788500T3/en active Active
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2016
- 2016-03-14 JP JP2016049365A patent/JP6149318B2/en active Active
- 2016-08-23 US US15/244,657 patent/US10626362B2/en active Active
Also Published As
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|---|---|
| US20160362651A1 (en) | 2016-12-15 |
| JP2013543738A (en) | 2013-12-09 |
| US10100273B2 (en) | 2018-10-16 |
| EP2643088B1 (en) | 2020-04-22 |
| JP6149318B2 (en) | 2017-06-21 |
| US10626362B2 (en) | 2020-04-21 |
| EP2643088A1 (en) | 2013-10-02 |
| EP3210670A1 (en) | 2017-08-30 |
| JP5961178B2 (en) | 2016-08-02 |
| ES2788500T3 (en) | 2020-10-21 |
| CN105802834A (en) | 2016-07-27 |
| JP2016105732A (en) | 2016-06-16 |
| PL2643088T3 (en) | 2020-08-24 |
| CN103221134B (en) | 2016-06-01 |
| DK3210670T3 (en) | 2019-03-11 |
| CN103221134A (en) | 2013-07-24 |
| PT2643088T (en) | 2020-05-06 |
| WO2012071249A1 (en) | 2012-05-31 |
| EP3210670B1 (en) | 2019-01-23 |
| US20120295343A1 (en) | 2012-11-22 |
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