CN214916228U - Centrifuging tube for cell culture - Google Patents

Abstract

The utility model discloses a centrifuging tube for cell culture, including the pipe box, the top of pipe box outer wall bonds there is the guard circle, pipe box inner wall gomphosis has the heat preservation, the inner tube is installed to the pipe box inner wall, through threaded ring threaded connection between pipe box and the inner tube, the visor is installed at the pipe box top, sealed lid circle is installed at visor bottom center, be connected through the sealing ring between sealed lid circle and the pipe box, the ring channel has been seted up at guard circle outer wall center. The detachable inner tube is designed to be used as a container for cell culture centrifugation, and the top of the inner tube is provided with the threaded ring to connect the inner tube with the top of the tube sleeve in a threaded manner, so that the inner tube is convenient to install and detach; the sealing cover ring 7 and the sealing ring 8 which are integrally designed can ensure that the inner pipe can obtain good sealing effect.

Description

Centrifuging tube for cell culture

Technical Field

The utility model relates to a centrifuging tube technical field especially relates to a centrifuging tube for cell culture.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Centrifuge tubes have found wide use in the centrifugal separation of biological sciences, particularly in the field of biochemical and molecular biological research, where many types of centrifuges are prepared in each biochemical and molecular biological laboratory. The centrifugal technology is mainly used for separating and preparing various biological samples, a biological sample suspension is placed in a centrifugal tube and rotates at a high speed, and suspended micro particles (such as organelles, the sediment of biological macromolecules and the like) are settled at a certain speed under the action of huge centrifugal force, so that the suspended micro particles and a solution are separated.

The existing centrifugal tubes are sealed by buckling plastic tube covers or columnar rubber plugs, and the sealing mode is easy to leak, so that the cell culture and centrifugation results are influenced; the body that current centrifuging tube all adopted the integral type design simultaneously damages easily, and when centrifuging tube carried out centrifugal operation in centrifuge simultaneously, the clamping structure in the centrifuge directly carried out the centre gripping to the centrifuging tube body, did not have the pipe box of protection to protect the centrifuging tube, and the operation carelessly will cause the breakage of centrifuging tube.

Therefore, it is desirable to provide a centrifuge tube for cell culture to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problem that current centrifuging tube all adopts buckled plastics tube cap or column plug to seal the mouth of pipe, and sealed effect is not too good, and current centrifuging tube is the integral type structure simultaneously, when carrying out centrifugal operation in centrifuge, does not have the pipe box of protection usefulness, and the operation carelessly will cause the breakage of centrifuging tube.

In order to solve the technical problem, the utility model discloses a technical scheme be: the centrifuge tube for cell culture comprises a tube sleeve, wherein a protective ring is bonded to the top end of the outer wall of the tube sleeve, the protective ring can play a role in protecting when the tube sleeve is held, a heat-insulating layer is embedded in the inner wall of the tube sleeve, and the heat-insulating layer can realize certain heat-insulating capacity for the inner tube;

the inner pipe is arranged on the inner wall of the pipe sleeve and serves as a pipe body for culturing cells, the pipe sleeve and the inner pipe are in threaded connection through a threaded ring, the inner pipe and the pipe sleeve are in threaded connection through the threaded ring, so that the inner pipe is convenient to install and detach, the protective cover is arranged at the top of the pipe sleeve and serves as a protective element of the sealing cover ring and the inner pipe, the sealing cover ring is arranged at the center of the bottom of the protective cover and is used for sealing and protecting the inner pipe, the cell culture solution in the inner pipe is prevented from leaking, the sealing cover ring is connected with the pipe sleeve through a sealing ring, and the sealing ring can be used for secondarily sealing the inner pipe;

an annular groove is formed in the center of the outer wall of the protection ring, and the annular groove can be matched with a clamping ring in the centrifugal machine to clamp and fix the pipe sleeve.

Preferably, the pipe sleeve is made of polycarbonate material, and the material is transparent, so that the conditions of cell culture and centrifugation in the inner pipe arranged on the inner wall of the pipe sleeve can be observed conveniently.

Preferably, the top of the pipe sleeve is provided with a thread groove corresponding to the thread ring.

Preferably, the inner pipe and the threaded ring are of an integrated structure, the integrated structure can enable the structure to be simple, and the installation and the disassembly are more convenient.

Preferably, the center of the top of the protective cover is provided with a through hole corresponding to the convex structure at the center of the top of the sealing cover ring, and the through hole can enable the injection needle tube to penetrate through the sealing cover ring to inject cell culture solution into the inner tube.

Preferably, the sealing cover ring and the sealing ring are of an integrated structure, and the sealing performance can be maximized by the integrated structure of the sealing cover ring and the sealing ring.

Preferably, the sealing cap ring is made of butyl material.

The utility model has the advantages as follows:

1. the utility model can protect the inner tube for cell culture installed on the inner wall through the designed pipe sleeve, and the heat preservation layer embedded on the inner wall of the pipe sleeve can have a certain heat preservation effect on the inner tube;

2. the detachable inner tube is designed to be used as a container for cell culture centrifugation, and the top of the inner tube is provided with the threaded ring to connect the inner tube with the top of the tube sleeve in a threaded manner, so that the inner tube is convenient to install and detach; the sealing cover ring 7 and the sealing ring 8 which are integrally designed can ensure that the inner pipe can obtain good sealing effect.

Drawings

FIG. 1 is a front view structural diagram of the present invention;

FIG. 2 is a front view cross-sectional structure of the present invention;

FIG. 3 is an enlarged view of a portion of FIG. 2 at A;

fig. 4 is a cross-sectional view of the middle sealing cover ring of the present invention.

In the figure: 1. pipe sleeve; 2. a guard ring; 3. a heat-insulating layer; 4. an inner tube; 5. a threaded ring; 6. a protective cover; 7. sealing the cover ring; 8. a seal ring; 9. an annular groove.

Detailed Description

The following detailed description of the preferred embodiments of the present invention will be provided in conjunction with the accompanying drawings, so as to enable those skilled in the art to more easily understand the advantages and features of the present invention, and thereby define the scope of the invention more clearly and clearly.

Referring to fig. 1 and 2, a centrifugal tube for cell culture comprises a tube sleeve 1, wherein the tube sleeve 1 is made of polycarbonate material, the material is transparent, cell culture and centrifugation conditions in an inner tube 4 mounted on the inner wall of the tube sleeve 1 can be conveniently observed, a thread groove corresponding to a thread ring 5 is formed in the top of the tube sleeve 1, a protective ring 2 is bonded to the top end of the outer wall of the tube sleeve 1, the protective ring 2 can play a role in protecting the tube sleeve 1 when the tube sleeve 1 is held, a heat-insulating layer 3 is embedded in the inner wall of the tube sleeve 1, and the heat-insulating layer 3 can realize certain heat-insulating capacity for the inner tube 4;

as shown in fig. 3, an inner tube 4 is installed on the inner wall of a tube housing 1, the inner tube 4 is used as a tube body for culturing cells, the tube housing 1 is in threaded connection with the inner tube 4 through a threaded ring 5, the inner tube 4 and the tube housing 1 are in threaded connection through the threaded ring 5 to facilitate the installation and the disassembly of the inner tube 4, the inner tube 4 and the threaded ring 5 are of an integrated structure, the integrated structure can simplify the structure and facilitate the installation and the disassembly, a protective cover 6 is installed on the top of the tube housing 1, the protective cover 6 is used as a protective element for a sealing cover ring 7 and the inner tube 4, a through hole corresponding to a convex structure at the top center of the sealing cover ring 7 is formed in the top center of the protective cover 6, the through hole can enable an injection needle tube to penetrate through the sealing cover ring 7 to inject cell culture fluid into the inner tube 4, as shown in fig. 4, the sealing cover ring 7 is installed at the bottom center of the protective cover 6 to seal and protect the inner tube 4 to prevent the cell culture fluid in the inner tube 4 from leaking, the sealing cover ring 7 is made of butyl materials, the sealing cover ring 7 is connected with the pipe sleeve 1 through the sealing ring 8, the sealing ring 8 can carry out secondary sealing on the inner pipe 4, the sealing cover ring 7 and the sealing ring 8 are of an integrated structure, and the sealing cover ring 7 and the sealing ring 8 are of an integrated structure, so that the sealing performance can be maximized;

an annular groove 9 is formed in the center of the outer wall of the protection ring 2, and the annular groove 9 can be matched with a clamping ring in a centrifugal machine to clamp and fix the pipe sleeve 1.

When the utility model is used, the protection ring 2 is bonded on the outer wall of the pipe sleeve 1, the inner pipe 4 is arranged at the center of the inner wall of the pipe sleeve 1, the inner pipe 4 is in threaded connection with the pipe sleeve 1 through the threaded ring 5, then the sealing cover ring 7 is arranged at the top of the pipe sleeve 1, the sealing cover ring 7 is in sealing connection with the pipe sleeve 1 through the sealing ring 8, finally the protection cover 6 is arranged at the top of the sealing cover ring 7, and the inner wall of the protection cover 6 is tightly combined with the top of the sealing cover ring 7; the cell culture fluid can be injected into the inner tube 4 by using an injection needle tube which penetrates through the sealing cover ring 7 and enters the inner tube 4 through a through hole which is formed in the center of the top of the protective cover 6 and corresponds to a protruding structure on the top of the sealing cover ring 7, or the cell culture fluid is injected into the inner tube 4 before sealing, then the protective cover 6 and the sealing cover ring 7 are installed, and the method for injecting the cell culture fluid can be selected according to the requirements of different experiments.

The above only is the embodiment of the present invention, not limiting the patent scope of the present invention, all the equivalent structures or equivalent processes that are used in the specification and the attached drawings or directly or indirectly applied to other related technical fields are included in the patent protection scope of the present invention.

Claims (7)

1. A centrifuge tube for cell culture comprises a tube sleeve (1), and is characterized in that: a protective ring (2) is bonded to the top end of the outer wall of the pipe sleeve (1), and a heat-insulating layer (3) is embedded in the inner wall of the pipe sleeve (1);

the inner pipe (4) is installed on the inner wall of the pipe sleeve (1), the pipe sleeve (1) is in threaded connection with the inner pipe (4) through a threaded ring (5), a protective cover (6) is installed at the top of the pipe sleeve (1), a sealing cover ring (7) is installed at the center of the bottom of the protective cover (6), and the sealing cover ring (7) is connected with the pipe sleeve (1) through a sealing ring (8);

an annular groove (9) is formed in the center of the outer wall of the protection ring (2).

2. The centrifugal tube for cell culture according to claim 1, wherein: the pipe sleeve (1) is made of polycarbonate material.

3. The centrifugal tube for cell culture according to claim 1, wherein: the top of the pipe sleeve (1) is provided with a thread groove corresponding to the thread ring (5).

4. The centrifugal tube for cell culture according to claim 1, wherein: the inner pipe (4) and the threaded ring (5) are of an integrated structure.

5. The centrifugal tube for cell culture according to claim 1, wherein: the center of the top of the protective cover (6) is provided with a through hole corresponding to the convex structure of the center of the top of the sealing cover ring (7).

6. The centrifugal tube for cell culture according to claim 1, wherein: the sealing cover ring (7) and the sealing ring (8) are of an integrated structure.

7. The centrifugal tube for cell culture according to claim 6, wherein: the sealing cover ring (7) is made of butyl materials.

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