CN213835371U - Cell culture centrifuge tube for enzyme-linked immunosorbent assay experiment - Google Patents

Abstract

The utility model discloses a cell culture centrifuge tube for enzyme-linked immunosorbent assay experiment, a plurality of puncture holes are arranged on a sealing plug, when adding sample into a culture medium, the sample can be directly added by an injector without unscrewing a connecting cover, and the contact part of the connecting cover and a tube body is provided with a thread structure, so that the connecting cover and the tube body can be fixedly connected by screwing; through setting up the elastic gasket, can make and connect lid and body close coupling. The utility model discloses a cell culture centrifuging tube for ELISA experiments, the application of sample is convenient when transferring the cultured cell, need not open the connection lid during the application of sample, and avoids the pollution at the transferred cell in-process, improves the cell culture success rate, ensures the result of ELISA experiments.

Description

Cell culture centrifuge tube for enzyme-linked immunosorbent assay experiment

Technical Field

The utility model belongs to the technical field of cell culture experimental device, in particular to cell culture centrifuging tube for ELISA experiments.

Background

The chicken immunoglobulin G (IgG) is obtained by taking chicken serum as a raw material and adopting ion exchange chromatography and the like, and when the IgG level in the chicken serum is measured by adopting an enzyme-linked immunosorbent assay (ELISA), a chicken serum sample needs to be subjected to cell culture and then to centrifugal operation.

The culture medium packaged by the existing glass bottle is mainly used in the glass bottle in the culture process, and after the culture is finished, cells are transferred into a centrifugal tube for operation. The problem of insufficient contact between cells and a culture medium exists in the process of cell culture in a glass bottle, and in addition, the process of transferring the cells to a centrifuge tube is complicated, so that the possibility of error and cross contamination exists, and the experimental result is influenced.

The culture medium of current standard centrifugation tube package, cell culture and later stage chromosome preparation all carry out in same tube, do not have the operation of transfer culture cell, nevertheless have the inconvenient problem of application of sample, need open the lid during the application of sample, carry out application of sample operation in superclean bench or biosafety cabinet, and there is the possibility of pollution.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a cell culture centrifuging tube for ELISA adsorption experiment, the application of sample is convenient when transferring the cultured cell, need not open the connection lid during the application of sample, and avoids the pollution at the transfer cell in-process, improves the cell culture success rate, ensures the result of ELISA experiment.

In order to achieve the above purpose, the solution of the present invention is:

a cell culture centrifuge tube for enzyme-linked immunosorbent assay experiments comprises a tube body, a connecting cover, a sealing plug and a top cap, wherein an opening is formed in the top end of the tube body, a culture medium is contained in the tube body, and scale marks and labels are arranged on the outer surface of the tube body; the connecting cover is annularly sleeved at the opening of the pipe body, and a buckle is arranged on the periphery of the connecting cover; the sealing plug is embedded into the opening of the pipe body and is provided with a plurality of puncture holes; the top cap is fixed at the bottom of the sealing plug, and the inner wall of the top cap is provided with a clamping groove matched with the buckle of the connecting cover.

An elastic gasket is arranged between the connecting cover and the pipe body, the elastic gasket is in a ring shape, the outer diameter of the elastic gasket is matched with the diameter of the inner wall of the connecting cover, and the inner diameter of the elastic gasket is matched with the diameter of the opening.

The outside cover of above-mentioned body has anti-skidding cover, and anti-skidding cover surface is equipped with anti-skidding line.

The inner wall of the connecting cover is provided with an internal thread, the pipe body is correspondingly provided with an external thread, and the connecting cover and the pipe body are connected through the thread.

The sealing plug is a butyl rubber sealing plug.

The bottom surface of the inner wall of the pipe body is an arc surface.

The inner cavity of the pipe body is provided with a pre-vacuum layer.

After the scheme is adopted, the utility model discloses compare prior art's beneficial effect is:

(1) the utility model discloses a set up a plurality of puncture holes at the sealing plug, can use the syringe to add the sample directly when adding the sample to the culture medium, need not to unscrew the connecting cover, and can not pollute the culture medium, improve the cell culture success rate, ensure the result of ELISA experiment;

(2) the utility model can lead the connecting cover and the pipe body to be fixedly connected by screwing through arranging the thread structures at the contact part of the connecting cover and the pipe body;

(3) the utility model can make the connecting cover and the pipe body combined closely by arranging the elastic gasket;

(4) the utility model discloses a preset a certain amount of vacuum negative pressure at the centrifuging tube, absorb the sample automatically, make the sample be in airtight state completely to the culture tube, both can pollution abatement, also increased the convenience of use, prevent that dust and bacterium from falling into the sealing plug top.

Drawings

Fig. 1 is a perspective view of the present invention;

FIG. 2 is an exploded view of the present invention;

FIG. 3 is a structural diagram of the connecting cover of the present invention;

fig. 4 is a structural view of the middle top hat of the present invention.

Reference numerals:

1. a pipe body; 101. an anti-slip sleeve;

2. a culture medium; 3. scale lines; 4. a label;

5. a connecting cover; 6. buckling; 7. an elastic pad;

8. an opening; 9. a sealing plug; 10. puncturing a hole;

11. a top cap; 12. a clamping groove.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.

As shown in fig. 1 to 4, the utility model provides a cell culture centrifuge tube for enzyme-linked immunosorbent assay, which comprises a tube body 1, wherein a culture medium 2 is arranged at the bottom of the tube body 1, a scale mark 3 is arranged on one side of the surface of the tube body 1, a label 4 is arranged on the other side of the tube body 1, a connecting cover 5 is fixedly arranged at the top end of the tube body 1, a buckle 6 is arranged on the outer side of the connecting cover 5, and an elastic gasket 7 is arranged between the connecting cover 5 and the tube body 1; an opening 8 is formed in the top end of the tube body 1, a sealing plug 9 is fixedly installed in the opening 8, and a puncture hole 10 is formed in the top end of the sealing plug 9; a top cap 11 is fixedly arranged at the top of the sealing plug 9, and a clamping groove 12 is formed in the inner wall of the top cap 11.

As a preferred embodiment of the present invention, as shown in fig. 2, the outer sleeve of the pipe body 1 has an anti-slip sleeve 101, the surface of the anti-slip sleeve 101 is provided with anti-slip lines, and the anti-slip sleeve 101 is a rubber anti-slip sleeve, and can prevent the anti-slip sleeve from slipping during the carrying process.

As a preferred embodiment of the present invention, as shown in fig. 2, the contact portion between the connection cover 5 and the pipe body 1 is provided with a threaded structure, the inner wall side of the connection cover 5 is provided with an internal threaded structure, the outer threaded structure is provided on one side of the pipe body 1, the connection cover 5 and the pipe body 1 are fixedly connected through the threaded structure, the connection cover and the pipe body contact portion are provided with a threaded structure, and the connection cover and the pipe body can be screwed and fixedly connected through the threaded structure.

As a preferred embodiment of the present invention, the elastic pad 7 is a rubber ring-shaped pad, the inner diameter of the elastic pad 7 is equal to the diameter of the opening 8, the outer diameter of the elastic pad 7 is equal to the diameter of the inner wall of the connection cover 5, and the connection cover 5 can be tightly combined with the pipe body 1 by providing the elastic pad 7.

As a preferred embodiment of the utility model, as shown in fig. 1, the sealing plug 9 is inlayed in body 1 through opening 8, and sealing plug 9 is a butyl rubber sealing plug, and sealing plug 9 top is provided with a plurality of puncture holes 10, through setting up a plurality of puncture holes, can need not to unscrew when adding appearance to culture medium 2 and connect lid 5, can use the direct application of sample of syringe, and can not pollute the culture medium.

As a preferred embodiment of the present invention, as shown in fig. 3 and 4, four sets of the slots 12 and the buckles 6 are provided, the slots 12 and the buckles 6 are engaged with each other, and the connecting cover 5 is fixedly connected to the top cap 11 by engaging the slots 12 and the buckles 6 with each other; through setting up draw-in groove 12 and buckle 6, can make connecting cover 5 and hood 11 fixed connection, prevent that dust and bacterium from falling into the sealing plug top.

As a preferred embodiment of the present invention, as shown in fig. 2, the bottom surface of the inner wall of the tube body 1 is an arc surface, the inner cavity of the tube body 1 is provided with a pre-vacuum layer, and the volume of the tube body 1 is 10-20 ml; through setting up the pre-vacuum layer, preset a certain amount of vacuum negative pressure through the centrifuging tube, absorb the sample automatically, make the sample be in airtight state completely to the centrifuging tube, both can pollution abatement, also increased the convenience in use.

The utility model discloses a theory of operation is: when the injector is in work, the top cap 11 is firstly opened, the injector is directly injected with sample through the puncture hole 10, and the anti-slip sleeve 101 is arranged, so that the tube body can be effectively prevented from slipping caused by hand slip in the carrying process; the contact part of the connecting cover 5 and the pipe body 1 is provided with the thread structures, so that the connecting cover 5 and the pipe body 1 can be fixedly connected through screwing of the thread structures; the connecting cover 5 can be tightly combined with the pipe body 1 by arranging the elastic gasket 7; by arranging the plurality of puncture holes 10, the sample can be directly added by using an injector without unscrewing a cover when the sample is added to the culture medium, and the culture medium is not polluted; the connecting cover 5 and the top cap 11 can be fixedly connected by arranging the clamping groove 12 and the buckle 6; preset a quantitative vacuum negative pressure through the culture tube, absorb the sample automatically, make the sample be in airtight state completely to the culture tube, both can the pollution abatement, also increased the convenience of use, prevent that dust and bacterium from falling into the sealing plug top. The utility model discloses the chromosome preparation that can effectively solve cell culture and later stage all goes on in same pipe, does not have the operation of transfer culture cell, nevertheless has the inconvenient problem of application of sample to and need open the lid during the application of sample in the current structure, carry out the application of sample operation in superclean bench or biosafety cabinet, and have the possibility of polluting, have higher practical value.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description of the present invention and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.

Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.

Claims (7)

1. A cell culture centrifuge tube for enzyme linked immunosorbent assay experiment, which is characterized in that: the culture medium container comprises a tube body, a connecting cover, a sealing plug and a top cap, wherein an opening is formed in the top end of the tube body, a culture medium is contained in the tube body, and scale marks and labels are arranged on the outer surface of the tube body; the connecting cover is annularly sleeved at the opening of the pipe body, and a buckle is arranged on the periphery of the connecting cover; the sealing plug is embedded into the opening of the pipe body and is provided with a plurality of puncture holes; the top cap is fixed at the bottom of the sealing plug, and the inner wall of the top cap is provided with a clamping groove matched with the buckle of the connecting cover.

2. The cell culture centrifuge tube for enzyme-linked immunosorbent assay of claim 1, wherein: an elastic gasket is arranged between the connecting cover and the pipe body, the elastic gasket is in a ring shape, the outer diameter of the elastic gasket is matched with the diameter of the inner wall of the connecting cover, and the inner diameter of the elastic gasket is matched with the diameter of the opening.

3. The cell culture centrifuge tube for enzyme-linked immunosorbent assay of claim 1, wherein: the outside cover of body has anti-skidding cover, and anti-skidding cover surface is equipped with anti-skidding line.

4. The cell culture centrifuge tube for enzyme-linked immunosorbent assay of claim 1, wherein: the inner wall of the connecting cover is provided with an internal thread, the pipe body is correspondingly provided with an external thread, and the connecting cover and the pipe body are connected through the thread.

5. The cell culture centrifuge tube for enzyme-linked immunosorbent assay of claim 1, wherein: the sealing plug is a butyl rubber sealing plug.

6. The cell culture centrifuge tube for enzyme-linked immunosorbent assay of claim 1, wherein: the bottom surface of the inner wall of the pipe body is an arc surface.

7. The cell culture centrifuge tube for enzyme-linked immunosorbent assay of claim 1, wherein: the inner cavity of the pipe body is provided with a pre-vacuum layer.

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