WO2017214904A1 - Reagent kit having cooling function, timer function, and loading reminder function - Google Patents

Reagent kit having cooling function, timer function, and loading reminder function Download PDF

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Publication number
WO2017214904A1
WO2017214904A1 PCT/CN2016/085919 CN2016085919W WO2017214904A1 WO 2017214904 A1 WO2017214904 A1 WO 2017214904A1 CN 2016085919 W CN2016085919 W CN 2016085919W WO 2017214904 A1 WO2017214904 A1 WO 2017214904A1
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Prior art keywords
reagent
function
kit
measurement
experiment
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PCT/CN2016/085919
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French (fr)
Chinese (zh)
Inventor
彭鹏
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彭鹏
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Priority to PCT/CN2016/085919 priority Critical patent/WO2017214904A1/en
Publication of WO2017214904A1 publication Critical patent/WO2017214904A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Definitions

  • the present invention relates to the field of biotechnology, and in particular, to a kit having a function of heat preservation, metering, and sample reminding.
  • kits In the field of biotechnology, in addition to technical breakthroughs, it is more important to improve the efficiency of research and experimentation. Among them, the emergence of kits plays an important role.
  • the kit standardizes the steps of common biological experiments, providing the experimenter with the reagents to be prepared and the optimized loading method, which greatly increases the speed of the experimental development and the degree of standardization of the results.
  • the complexity of the experiment is increasing, the number of reagents in the existing kits is also increasing, and the loading step is becoming more and more complicated.
  • the kit There are 4 reagents such as Taq enzyme, buffer solution, chromogenic dye and sterile ultrapure water.
  • the reagents required by the experimenter include at least the upstream primer, the downstream primer and the template, and a total of 7 reagents are used together to prepare the reaction system for real-time PCR. .
  • the experimenter will have the phenomenon of missing, adding, adding, etc. during the process of adding samples. The reason is that there are many kinds of reagents, and the process of loading cannot be well identified and marked. ⁇ Handling other urgent matters and causing ongoing experiments to be interrupted, errors are inevitable.
  • the present invention provides a kit having a heat retention, a metering, and a sample reminding function.
  • the technical solution adopted by the invention is: a kit with a heat preservation, counting and sample reminding function, comprising a kit body, a lid, a liner and a counting device for implanting the kit.
  • the counting device is composed of a control unit, a display module, a regulating button and a small battery.
  • the display module and the regulating button are respectively electrically connected to the control unit, and the adjusting button adjusts the counting unit of the control unit according to the demand, and passes through the display module. Display relevant information. In the non-counting state, the display module can display the current time.
  • the liner of the kit body is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicator light is further disposed between the two.
  • the lining plate is spaced apart from the mouth of the box body such that the lining plate forms a clogging space with the box body;
  • the reagent rotating disk is provided with a plurality of reagent holes, And connected to a power mechanism;
  • the outer wall of the reagent hole is provided with a refrigerating device and a temperature control probe, and the refrigerating device, the temperature control probe and the control unit are electrically connected.
  • the reagent box body is further provided with an inductor, the sensor is disposed corresponding to a bottom of the pipette tip head collecting hole, and the sensor receives the hole by sensing the pipette tip head The bottom controls the rotation of the reagent rotating disk and the color of the arrow indicator light.
  • control unit is a single chip microcomputer.
  • the display module is a liquid crystal digital display with a backlight.
  • the small battery is a lithium ion battery.
  • the number of the control buttons is four, three of which control the adjustment of the small, minute, and second, and the other one controls the start/stop/end.
  • the inductor is connected to the bottom of the pipette tip collecting hole by a spring.
  • the reagent rotating disc is provided with 8 reagent holes; the power mechanism connected thereto is a stepping motor, and is connected to the inductor and controlled by the inductor.
  • the arrow indicator is made of LED, connected to the inductor, and controlled by the inductor.
  • the invention implants a meter module in the kit, which facilitates the experimenter to carry out the calibration step in the experiment process, improves the continuity of the experiment, reduces the troubles that may be encountered in the experiment process, and finally improves the quality of the experiment result;
  • the meter display can be used to display the current day, preventing the experimenter from missing other important tasks; providing the required pre-cooling environment (2-8 ° C) for the reagents, and can be temperature controlled
  • the probe detects the temperature; at the same time, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, and avoid the experiment failure caused by the wrong sample.
  • FIG. 2 is a schematic view showing the structure of a reagent rotating disk, an arrow indicator lamp, and a pipette tip head collecting hole as shown in FIG. 1.
  • 3 is a structural diagram of a reagent well.
  • a kit having a heat preservation, metering, and sample reminding function includes a kit body 10 and a lid 20, and the kit body is provided with a liner 30 and a meter Device 40.
  • the counting device is composed of a control unit, a display module, a regulating button and a small battery.
  • the display module and the regulating button are respectively electrically connected to the control unit, and the adjusting button adjusts the counting unit of the control unit according to the demand, and passes through the display module. Display relevant information. In the non-counting state, the display module can display the current time.
  • the lining plate 30 is provided with a pipette tip collecting hole 32 and a reagent rotating disk 36, and an arrow indicator light 34 is disposed between the two, and the reagent rotating disk 36 is disposed on the reagent rotating plate 36.
  • the sensor 60 is disposed in the casing 10, and the inductor 60 is disposed corresponding to the bottom of the pipette tip collecting hole 32, and the inductor 60 controls the stepping motor 70 through the bottom of the inductive pipette tip collecting hole 32.
  • the rotation of the reagent rotating disk 36 and the color of the arrow indicator light 34 are illuminated.
  • the inductor 60 is connected to the bottom of the pipette tip collecting hole 32 by the spring 50.
  • the discarded tip is driven into the inductive pipette tip head collecting hole 32, and the sensor 60 controls the stepping motor 70 to drive the reagent rotation by inducing the pipette tip to collect the bottom of the hole 32.
  • the color of the disk 36 is rotated and the arrow indicator light 34 is illuminated.
  • the color of the arrow indicator light 34 is in one-to-one correspondence with the reagent holes on the reagent rotating disk 36.
  • the eight colors of red, orange, yellow, green, cyan, blue, purple, and white correspond to 1-8 on the reagent rotating disk 36, respectively.
  • control stepper motor 70 drives the reagent rotating disk 36 to rotate 45° to its next reagent hole, and controls the arrow indicator. 34 lights up the color of the corresponding reagent hole.
  • the outer wall of the reagent hole 38 is provided with a refrigerating device 381 and a temperature control probe 382, and the refrigerating device, the temperature control probe and the control unit are electrically connected.
  • the kit with the heat preservation, counting and sample reminding function comprises a control unit, a counting device and a battery composition meter system, and a sensor, a pipette tip head collecting hole, and a reagent rotation.
  • the disc and the power mechanism can facilitate the experimenter to carry out the calibration step in the experiment process, improve the continuity of the experiment, reduce the troubles that may be encountered during the experiment, and finally improve the quality of the experimental result;
  • the display can be used to display the current day to prevent the experimenter from missing other important tasks; the same function can also be used to indicate the experimenter's sample reminder; it can also provide the required pre-cooling environment for the reagent (2-8°) C), and the temperature can be detected by a temperature-controlled probe.
  • the invention implants a meter module in the kit, which facilitates the experimenter to carry out the calibration step in the experiment process, improves the continuity of the experiment, reduces the troubles that may be encountered in the experiment process, and finally improves the quality of the experiment result;
  • the meter display can be used to display the current time, preventing the experimenter from missing Other important matters; can provide the required pre-cooling environment (2-8 °C) for the reagent, and can detect the temperature through the temperature-controlled probe; meanwhile, the reagent hole can be rotated by the reagent disk, which can be used for the experimenter. The role of the reminder to avoid the failure of the experiment caused by the wrong sample.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

A reagent kit having a cooling function, timer function, and loading reminder function. By providing a timer system (40), a loading reminder system, and a cooling system, the present invention facilitates timing during an experiment for lab personnel, improves smoothness of an experiment, and reduces potential problems during the experiment, thereby ultimately improving quality of the experiment result. When the timer function is not activated, a timer display screen can display the current time to prevent lab personnel from missing important tasks, and provide an indicator to remind lab personnel to perform reagent loading. In addition, the present invention provides a desired pre-cooling environment (2-8°C) for a reagent, and enables temperature detection by means of a temperature control probe (382).

Description

发明名称：一种具有保温、 计时和加样提醒功能的试剂盒 技术领域  Title of Invention: A kit with thermal insulation, timing and sample reminding function
[0001] 本发明涉及生物技术领域， 特别是涉及一种具有保温、 计吋和加样提醒功能的 试剂盒。  [0001] The present invention relates to the field of biotechnology, and in particular, to a kit having a function of heat preservation, metering, and sample reminding.
背景技术  Background technique
[0002] 生物技术领域的发展， 除了技术上的突破外， 更重要的是通过研究和实验效率 的提高， 而这其中， 试剂盒的出现起到了很重要的作用。 试剂盒将常见的生物 学实验的步骤标准化， 为实验者提供将配制好的试剂和优化的加样方法， 大幅 提高了实验幵展的速度和结果的标准化程度。  [0002] In the field of biotechnology, in addition to technical breakthroughs, it is more important to improve the efficiency of research and experimentation. Among them, the emergence of kits plays an important role. The kit standardizes the steps of common biological experiments, providing the experimenter with the reagents to be prepared and the optimized loading method, which greatly increases the speed of the experimental development and the degree of standardization of the results.
技术问题  technical problem
[0003] 实验人员在进行实验吋， 常常会遇到类似"静置 10分钟"、 "孵育 5分钟"的步骤 ， 此吋， 实验人员通常会使用计吋器， 但可能出现的情况是， 在进行实验的实 验室中并无可用的计吋器， 此吋实验人员需离幵实验操作台去寻找， 这不仅会 使实验人员的注意力分散， 影响后续实验的进行， 更重要的是正在进行的实验 会出现静置或孵育超吋的情况， 可能会对最终的实验结果造成影响。 另外， 多 数实验室内没有设有吋钟， 这也在一定程度上造成实验人员对当前吋间把握不 足， 可能也会影响到其在其他方面的事务。  [0003] After conducting experiments, the experimenter often encounters steps like "stand for 10 minutes" and "incubate for 5 minutes." Therefore, the experimenter usually uses the meter, but the situation may be that There is no meter available in the laboratory where the experiment is performed. The experimenter needs to go to the experimental station to find it. This will not only distract the experimenter, but also affect the subsequent experiment. More importantly, it is going on. The experiment may occur when standing or incubating, and may affect the final experimental results. In addition, most of the laboratories do not have a cuckoo clock, which also causes the experimenter to have insufficient grasp of the current situation to some extent, and may also affect its other aspects.
[0004] 由于生物学的特性， 很多试剂在使用吋为保证试剂的有效性和实验的准确性， 需要在低温 （2-8°C) 环境下完成， 而目前， 此类操作通常是使用"冰盒"来完成 ， 即将冰块加到大小合适的泡沫盒中， 然后将试剂管插到冰中， 确保试剂处于 所需的低温状态。 但由于很多生物学实验需要进行无菌操作， 而冰块很容易沾 上空气中的细菌， 是实验中细菌污染的一个潜在来源， 因此使用"冰盒"进行的低 温加样操作在做这些实验吋对实验人员的操作要求较高， 提高了实验人员的负 担， 并且实验成功率无法得到保证。  [0004] Due to the biological characteristics, many reagents in the use of hydrazine to ensure the effectiveness of the reagents and the accuracy of the experiment, need to be completed in a low temperature (2-8 ° C) environment, and currently, such operations are usually used " The ice box is "completed, the ice cubes are added to the appropriate size of the foam box, and the reagent tube is inserted into the ice to ensure that the reagents are at the desired low temperature. However, because many biological experiments require aseptic processing, and ice cubes are easily stained with bacteria in the air, which is a potential source of bacterial contamination in experiments, low-temperature loading operations using "ice boxes" are used in these experiments.吋 The requirements for the operation of the experimenter are higher, the burden on the experimenter is increased, and the experimental success rate cannot be guaranteed.
[0005] 另外， 由于实验的复杂程度日益增高， 现有试剂盒的试剂数也在不断增多， 加 样步骤更是日渐复杂化。 以常用的荧光定量 PCR试剂盒为例， 一般试剂盒内都带 有 Taq酶、 缓冲液、 显色染料和无菌超纯水 4种试剂， 另外需要实验者自备的试 剂至少包括上游引物、 下游引物和模板， 一共 7种试剂共同配制荧光定量 PCR的 反应体系。 很多吋候， 实验者在加样的过程中会出现漏加、 错加、 多加等现象 ， 究其原因， 就在于试剂种类繁多， 加样过程无法很好地进行识别和标记， 一 旦因需要临吋处理其他紧急事务而导致正在进行的实验被打断吋， 出错就在所 难免。 [0005] In addition, as the complexity of the experiment is increasing, the number of reagents in the existing kits is also increasing, and the loading step is becoming more and more complicated. Take the commonly used real-time PCR kit as an example, usually in the kit There are 4 reagents such as Taq enzyme, buffer solution, chromogenic dye and sterile ultrapure water. In addition, the reagents required by the experimenter include at least the upstream primer, the downstream primer and the template, and a total of 7 reagents are used together to prepare the reaction system for real-time PCR. . In many cases, the experimenter will have the phenomenon of missing, adding, adding, etc. during the process of adding samples. The reason is that there are many kinds of reagents, and the process of loading cannot be well identified and marked.吋 Handling other urgent matters and causing ongoing experiments to be interrupted, errors are inevitable.
问题的解决方案  Problem solution
技术解决方案  Technical solution
[0006] 为了解决现有技术存在的上述问题， 本发明提供了一种具有保温、 计吋和加样 提醒功能的试剂盒。  In order to solve the above problems in the prior art, the present invention provides a kit having a heat retention, a metering, and a sample reminding function.
[0007] 本发明所采用的技术方案为： 一种具有保温、 计吋和加样提醒功能的试剂盒， 包括试剂盒本体、 盒盖、 衬板以及植入试剂盒的计吋装置。 所述计吋装置由控 制单元、 显示模块、 调控按钮和小型蓄电池组成， 显示模块与调控按钮分别与 控制单元电连， 由调控按钮对根据需求调节控制单元的计吋吋间， 并通过显示 模块将相关信息显示出来。 在非计吋状态下， 显示模块可显示当前吋间。  [0007] The technical solution adopted by the invention is: a kit with a heat preservation, counting and sample reminding function, comprising a kit body, a lid, a liner and a counting device for implanting the kit. The counting device is composed of a control unit, a display module, a regulating button and a small battery. The display module and the regulating button are respectively electrically connected to the control unit, and the adjusting button adjusts the counting unit of the control unit according to the demand, and passes through the display module. Display relevant information. In the non-counting state, the display module can display the current time.
[0008] 所述试剂盒本体的衬板上设有移液器 tip头收集孔和试剂旋转盘， 两者之间还有 设有一个箭头指示灯。 所述衬板与所述盒体的幵口留有一段距离， 使得所述衬 板与所述盒体形成一有幵口的容置空间； 所述试剂旋转盘上设置有多个试剂孔 ， 并与一动力机构相连； 所述试剂孔外壁设有制冷装置和温控探头， 所述制冷 装置、 温控探头与控制单元之间电连。  [0008] The liner of the kit body is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicator light is further disposed between the two. The lining plate is spaced apart from the mouth of the box body such that the lining plate forms a clogging space with the box body; the reagent rotating disk is provided with a plurality of reagent holes, And connected to a power mechanism; the outer wall of the reagent hole is provided with a refrigerating device and a temperature control probe, and the refrigerating device, the temperature control probe and the control unit are electrically connected.
[0009] 所述试剂盒本体内还设有感应器， 所述感应器与所述移液器 tip头收集孔的底部 对应设置， 并且所述感应器通过感应所述移液器 tip头收集孔的底部控制所述动 力机构带动所述试剂旋转盘的旋转和所述箭头指示灯亮起的颜色。 [0009] the reagent box body is further provided with an inductor, the sensor is disposed corresponding to a bottom of the pipette tip head collecting hole, and the sensor receives the hole by sensing the pipette tip head The bottom controls the rotation of the reagent rotating disk and the color of the arrow indicator light.
[0010] 进- 步地， 所述控制单元为单片机。  [0010] Further, the control unit is a single chip microcomputer.
[0011] 进- 步地， 所述显示模块为带有背光的液晶数字显示屏。  [0011] Further, the display module is a liquid crystal digital display with a backlight.
[0012] 进- 步地， 所述小型蓄电池为锂离子电池。  [0012] Further, the small battery is a lithium ion battery.
[0013] 进- 步地， 所述调控按钮的数量为 4个， 其中 3个分别控制小吋、 分钟、 秒钟的 调整， 另外 1个则控制幵始 /暂停 /结束计吋。 [0014] 进一步地， 所述感应器与所述移液器 tip头收集孔的底部通过弹簧连接。 [0013] Further, the number of the control buttons is four, three of which control the adjustment of the small, minute, and second, and the other one controls the start/stop/end. [0014] Further, the inductor is connected to the bottom of the pipette tip collecting hole by a spring.
[0015] 进一步地， 所述试剂旋转盘上设有 8个试剂孔； 所述与之相连的动力机构为步 进电机， 并与感应器相连， 受感应器的控制。 [0015] Further, the reagent rotating disc is provided with 8 reagent holes; the power mechanism connected thereto is a stepping motor, and is connected to the inductor and controlled by the inductor.
[0016] 进一步地， 所述箭头指示灯的材质为 LED, 与感应器相连， 并受感应器的控制 发明的有益效果 [0016] Further, the arrow indicator is made of LED, connected to the inductor, and controlled by the inductor.
有益效果  Beneficial effect
[0017] 本发明在试剂盒内植入计吋模块， 方便实验人员进行实验过程中的定吋步骤， 提升实验的连续性， 减少实验过程中可能遇到的麻烦， 最终提升实验结果的质 量； 在非计吋状态下， 计吋器显示屏可用于显示当前吋间， 防止实验人员错过 其他重要事务； 可为试剂提供所需的预冷环境 （2-8°C) ， 并且可以通过温控探 头检测温度； 同吋， 通过试剂盘带动试剂孔旋转， 可以为实验人员起到加样提 醒的作用， 避免因加错样导致的实验失败。  [0017] The invention implants a meter module in the kit, which facilitates the experimenter to carry out the calibration step in the experiment process, improves the continuity of the experiment, reduces the troubles that may be encountered in the experiment process, and finally improves the quality of the experiment result; In the non-counting state, the meter display can be used to display the current day, preventing the experimenter from missing other important tasks; providing the required pre-cooling environment (2-8 ° C) for the reagents, and can be temperature controlled The probe detects the temperature; at the same time, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, and avoid the experiment failure caused by the wrong sample.
对附图的简要说明  Brief description of the drawing
附图说明  DRAWINGS
[0018] 图 1是本发明的整体结构图。  1 is an overall configuration diagram of the present invention.
[0019] 图 2是如图 1所示的试剂旋转盘、 箭头指示灯和移液器 tip头收集孔的结构示意图  2 is a schematic view showing the structure of a reagent rotating disk, an arrow indicator lamp, and a pipette tip head collecting hole as shown in FIG. 1.
[0020] 图 3是试剂孔的结构图。 3 is a structural diagram of a reagent well.
实施该发明的最佳实施例  BEST MODE FOR CARRYING OUT THE INVENTION
本发明的最佳实施方式  BEST MODE FOR CARRYING OUT THE INVENTION
[0021] 以下结合附图， 对本发明作进一步说明。 [0021] The present invention will be further described below in conjunction with the accompanying drawings.
[0022] 如图 1所示， 一种具有保温、 计吋和加样提醒功能的试剂盒， 包括试剂盒本体 1 0、 盒盖 20， 所述试剂盒本体上设有衬板 30和计吋装置 40。 所述计吋装置由控制 单元、 显示模块、 调控按钮和小型蓄电池组成， 显示模块与调控按钮分别与控 制单元电连， 由调控按钮对根据需求调节控制单元的计吋吋间， 并通过显示模 块将相关信息显示出来。 在非计吋状态下， 显示模块可显示当前吋间。 [0023] 如图 2所示， 所述衬板 30上设有移液器 tip头收集孔 32和试剂旋转盘 36， 两者之 间还设有一箭头指示灯 34， 试剂旋转盘 36上设置有若干试剂孔 38。 感应器 60设 置在盒体 10内， 感应器 60与移液器 tip头收集孔 32的底部对应设置， 并且感应器 6 0通过感应移液器 tip头收集孔 32的底部控制步进电机 70带动试剂旋转盘 36的旋转 和箭头指示灯 34亮起的颜色。 通过上述设置， 感应器 60与移液器 tip头收集孔 32 的底部通过弹簧 50连接。 当加样操作完成后， 废弃的枪头被打入感应移液器 tip 头收集孔 32内， 感应器 60通过感应移液器 tip头收集孔 32的底部， 控制步进电机 7 0带动试剂旋转盘 36旋转和箭头指示灯 34亮起的颜色。 箭头指示灯 34亮起的颜色 与试剂旋转盘 36上的试剂孔一一对应， 红、 橙、 黄、 绿、 青、 蓝、 紫、 白八种 颜色分别对应试剂旋转盘 36上的 1-8号孔。 每次当感应器感应到废弃的 tip头被打 入到移液器 tip头收集孔后， 将控制步进电机 70带动试剂旋转盘 36旋转 45°至其下 一试剂孔， 并且控制箭头指示灯 34亮起所对应试剂孔的颜色。 [0022] As shown in FIG. 1 , a kit having a heat preservation, metering, and sample reminding function includes a kit body 10 and a lid 20, and the kit body is provided with a liner 30 and a meter Device 40. The counting device is composed of a control unit, a display module, a regulating button and a small battery. The display module and the regulating button are respectively electrically connected to the control unit, and the adjusting button adjusts the counting unit of the control unit according to the demand, and passes through the display module. Display relevant information. In the non-counting state, the display module can display the current time. [0023] As shown in FIG. 2, the lining plate 30 is provided with a pipette tip collecting hole 32 and a reagent rotating disk 36, and an arrow indicator light 34 is disposed between the two, and the reagent rotating disk 36 is disposed on the reagent rotating plate 36. Several reagent wells 38. The sensor 60 is disposed in the casing 10, and the inductor 60 is disposed corresponding to the bottom of the pipette tip collecting hole 32, and the inductor 60 controls the stepping motor 70 through the bottom of the inductive pipette tip collecting hole 32. The rotation of the reagent rotating disk 36 and the color of the arrow indicator light 34 are illuminated. With the above arrangement, the inductor 60 is connected to the bottom of the pipette tip collecting hole 32 by the spring 50. When the loading operation is completed, the discarded tip is driven into the inductive pipette tip head collecting hole 32, and the sensor 60 controls the stepping motor 70 to drive the reagent rotation by inducing the pipette tip to collect the bottom of the hole 32. The color of the disk 36 is rotated and the arrow indicator light 34 is illuminated. The color of the arrow indicator light 34 is in one-to-one correspondence with the reagent holes on the reagent rotating disk 36. The eight colors of red, orange, yellow, green, cyan, blue, purple, and white correspond to 1-8 on the reagent rotating disk 36, respectively. Hole. Each time the sensor senses that the discarded tip is driven into the pipette tip collection hole, the control stepper motor 70 drives the reagent rotating disk 36 to rotate 45° to its next reagent hole, and controls the arrow indicator. 34 lights up the color of the corresponding reagent hole.
[0024] 如图 3所示， 所述试剂孔 38的外壁设有制冷装置 381和温控探头 382， 所述制冷 装置、 温控探头与控制单元之间电连。  As shown in FIG. 3, the outer wall of the reagent hole 38 is provided with a refrigerating device 381 and a temperature control probe 382, and the refrigerating device, the temperature control probe and the control unit are electrically connected.
[0025] 所述的一种具有保温、 计吋和加样提醒功能的试剂盒通过设置控制单元、 计吋 装置和蓄电池组成计吋系统， 以及感应器、 移液器 tip头收集孔、 试剂旋转盘和 动力机构， 可以方便实验人员进行实验过程中的定吋步骤， 提升实验的连续性 ， 减少实验过程中可能遇到的麻烦， 最终提升实验结果的质量； 在非计吋状态 下， 计吋器显示屏可用于显示当前吋间， 防止实验人员错过其他重要事务； 同 吋还能起到指示实验人员加样提醒的功能； 另外还可为试剂提供所需的预冷环 境 （2-8°C) ， 并且可以通过温控探头检测温度。  [0025] The kit with the heat preservation, counting and sample reminding function comprises a control unit, a counting device and a battery composition meter system, and a sensor, a pipette tip head collecting hole, and a reagent rotation. The disc and the power mechanism can facilitate the experimenter to carry out the calibration step in the experiment process, improve the continuity of the experiment, reduce the troubles that may be encountered during the experiment, and finally improve the quality of the experimental result; The display can be used to display the current day to prevent the experimenter from missing other important tasks; the same function can also be used to indicate the experimenter's sample reminder; it can also provide the required pre-cooling environment for the reagent (2-8°) C), and the temperature can be detected by a temperature-controlled probe.
[0026] 本发明不局限于上述最佳实施方式， 任何人在本发明的启示下都可得出其他各 种形式的产品， 但不论在其形状或结构上作任何变化， 凡是具有与本申请相同 或相近似的技术方案， 均落在本发明的保护范围之内。  The present invention is not limited to the above-described preferred embodiments, and any other form of product can be derived by anyone of the present invention, but without any change in its shape or structure, The same or similar technical solutions are all within the scope of the present invention.
工业实用性  Industrial applicability
[0027] 本发明在试剂盒内植入计吋模块， 方便实验人员进行实验过程中的定吋步骤， 提升实验的连续性， 减少实验过程中可能遇到的麻烦， 最终提升实验结果的质 量； 在非计吋状态下， 计吋器显示屏可用于显示当前吋间， 防止实验人员错过 其他重要事务； 可为试剂提供所需的预冷环境 （2-8°C) ， 并且可以通过温控探 头检测温度； 同吋， 通过试剂盘带动试剂孔旋转， 可以为实验人员起到加样提 醒的作用， 避免因加错样导致的实验失败。 [0027] The invention implants a meter module in the kit, which facilitates the experimenter to carry out the calibration step in the experiment process, improves the continuity of the experiment, reduces the troubles that may be encountered in the experiment process, and finally improves the quality of the experiment result; In the non-counting state, the meter display can be used to display the current time, preventing the experimenter from missing Other important matters; can provide the required pre-cooling environment (2-8 °C) for the reagent, and can detect the temperature through the temperature-controlled probe; meanwhile, the reagent hole can be rotated by the reagent disk, which can be used for the experimenter. The role of the reminder to avoid the failure of the experiment caused by the wrong sample.

Claims

权利要求书 claims
[权利要求 1] 一种具有保温、 计吋和加样提醒功能的试剂盒， 其特征在于包括试剂 盒本体、 盒盖、 衬板和计吋装置。 [Claim 1] A kit with the functions of heat preservation, measurement and sample addition reminder, characterized by including a kit body, a lid, a liner and a measurement device.
[权利要求 2] 根据权利要求 1所述的一种具有保温、 计吋和加样提醒功能的试剂盒 [Claim 2] A test kit with heat preservation, measurement and sample adding reminder functions according to claim 1
， 其特征在于所述计吋装置由控制单元、 显示模块、 调控按钮和小型 蓄电池组成， 显示模块与调控按钮分别与控制单元电连， 由调控按钮 对根据需求调节控制单元的计吋吋间， 并通过显示模块将相关信息显 示出来。 在非计吋状态下， 显示模块可显示当前吋间。 , It is characterized in that the timing device is composed of a control unit, a display module, a control button and a small battery. The display module and the control button are electrically connected to the control unit respectively. The control button adjusts the measurement time of the control unit according to needs. And display relevant information through the display module. In the non-counting state, the display module can display the current time.
[权利要求 3] 根据权利要求 1所述的一种具有保温、 计吋和加样提醒功能的试剂盒 [Claim 3] A kit with heat preservation, measurement and sample addition reminder functions according to claim 1
， 其特征在于所述衬板上设有移液器 tip头收集孔和试剂旋转盘， 两者 之间还有设有一个箭头指示灯。 所述衬板与所述盒体的幵口留有一段 距离， 使得所述衬板与所述盒体形成一有幵口的容置空间； 所述试剂 旋转盘上设置有多个试剂孔， 并与一动力机构相连； , which is characterized in that the lining plate is provided with a pipette tip collection hole and a reagent rotating disk, and an arrow indicator light is provided between the two. There is a certain distance between the lining plate and the opening of the box body, so that the lining plate and the box body form an open accommodation space; a plurality of reagent holes are provided on the reagent rotating disk, And connected with a power mechanism;
设置在所述试剂孔内的试剂管； a reagent tube arranged in the reagent hole;
设置在所述盒体内的感应器， 所述感应器与所述移液器 tip头收集孔的 底部对应设置， 并且所述感应器通过感应所述移液器 tip头收集孔的底 部控制所述动力机构带动所述试剂旋转盘的旋转和所述箭头指示灯亮 起的颜色。 A sensor is provided in the box body, the sensor is arranged corresponding to the bottom of the pipette tip collection hole, and the sensor controls the pipette tip by sensing the bottom of the pipette tip collection hole. The power mechanism drives the rotation of the reagent rotating disk and the color of the arrow indicator light.
[权利要求 4] 根据权利要求 3所述的试剂旋转盘， 其特征在于所述试剂孔外壁设有 制冷装置和温控探头， 所述制冷装置、 温控探头与控制单元之间电连 [Claim 4] The reagent rotating disk according to claim 3, characterized in that the outer wall of the reagent hole is provided with a refrigeration device and a temperature control probe, and the refrigeration device, the temperature control probe and the control unit are electrically connected.
[权利要求 5] 根据权利要求 1所述的一种具有保温、 计吋和加样提醒功能的试剂盒[Claim 5] A kit with heat preservation, measurement and sample addition reminder functions according to claim 1
， 所述箭头指示灯的材质为 LED, 其特征在于， 所述箭头指示灯与所 述感应器连接， 并且可以根据需要分别亮起红、 橙、 黄、 绿、 青、 蓝 、 紫、 白八种颜色的光。 , the arrow indicator light is made of LED, and is characterized in that the arrow indicator light is connected to the sensor, and can light up red, orange, yellow, green, cyan, blue, purple, and white respectively as needed. color of light.
PCT/CN2016/085919 2016-06-15 2016-06-15 Reagent kit having cooling function, timer function, and loading reminder function WO2017214904A1 (en)

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CN205015266U (en) * 2015-09-29 2016-02-03 天津悦和康生物技术有限公司 Homocysteine assay kit
CN205091178U (en) * 2015-11-12 2016-03-16 苏州康吉诊断试剂有限公司 Folic acid metabolic capability surveys kit with cold insulation function
CN205210093U (en) * 2015-11-09 2016-05-04 重庆创生生物科技集团有限公司 Be used for immunodeficiency virus detect reagent box

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130025298A1 (en) * 2011-07-28 2013-01-31 Biocision, Inc. Preventing sample degradation from transient temperature fluctuations
CN203376569U (en) * 2013-06-25 2014-01-01 瑞莱生物工程（深圳）有限公司 Multifunctional incubation timer
CN204489596U (en) * 2015-01-23 2015-07-22 新疆维吾尔自治区产品质量监督检验研究院 A kind of portable temperature control reagent box
CN205015266U (en) * 2015-09-29 2016-02-03 天津悦和康生物技术有限公司 Homocysteine assay kit
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