WO2017214865A1

WO2017214865A1 - Reagent kit having timer function, loading reminder function, and wireless management function

Info

Publication number: WO2017214865A1
Application number: PCT/CN2016/085767
Authority: WO
Inventors: 彭鹏
Original assignee: 彭鹏
Priority date: 2016-06-15
Filing date: 2016-06-15
Publication date: 2017-12-21

Abstract

A reagent kit having a timer function, loading reminder function, and wireless management function. By providing a timer system consisting of a control unit, a timer device (40), and a storage battery, and providing a sensor (6), a pipette tip collector (32), a reagent turntable (36), and a power mechanism, the present invention facilitates timing during an experiment for lab personnel, thus improving smoothness of an experiment, reducing potential problems during the experiment, and ultimately improving quality of the experiment result. When the timer function is not activated, a timer display screen can display the current time to prevent lab personnel from missing important tasks, and provide an indicator to remind lab personnel to load a reagent. In addition, by employing contactless end-to-end data transmission between an intelligent management terminal and the reagent kit within a five-centimeter range, the present invention solves numerous problems of manufacturing management methods of conventional reagent kits, thus improving efficiency and precision of management, and reducing a management cost of the manufacturer.

Description

Kit with timing, sample reminder and wireless management function

[0001] The present invention relates to the field of biotechnology, and more particularly to a kit having a metering, sample reminder, and wireless management function.

Background technique

[0002] In the field of biotechnology, in addition to technical breakthroughs, it is more important to improve the efficiency of research and experimentation. Among them, the emergence of kits plays an important role. The kit standardizes the steps of common biological experiments, providing the experimenter with the reagents to be prepared and the optimized loading method, which greatly increases the speed of the experiment and the standardization of the results.

technical problem

[0003] When experimenting, the experimenter often encounters steps like "stand for 10 minutes" and "incubate for 5 minutes." At this time, the experimenter usually uses a timer, and the possible situation is that There is no timer available in the laboratory. At this point, the experimenter needs to leave the experimental console to find it. This will not only distract the experimenter, but also affect the subsequent experiment. More importantly, the ongoing experiment will The presence of a standstill or incubation timeout may have an impact on the final experimental results. In addition, most of the laboratories do not have a clock, which also causes the experimenter to grasp the current time to a certain extent, and may also affect its other aspects.

[0004] In addition, as the complexity of the experiment is increasing, the number of reagents in the existing kits is also increasing, and the step of adding samples is becoming more and more complicated. Take the commonly used real-time PCR kit as an example. The general kit contains Taq enzyme, buffer, chromogenic dye and sterile ultrapure water. In addition, the reagents required by the experimenter include at least the upstream primer. Downstream primers and template, a total of 7 reagents together to prepare a fluorescent quantitative PCR reaction system. In many cases, the experimenter will have the phenomenon of missing, adding, adding, etc. during the process of adding samples. The reason is that there are many kinds of reagents, and the loading process cannot be well identified and marked. When other urgent matters cause the ongoing experiment to be interrupted, an error is inevitable.

[0005] With the development of biology and the interweaving of biology and other closely related disciplines, the number of interdisciplinary studies has increased. The types of kits are also constantly increasing. For the manufacturers of kits, especially for large manufacturers that produce a wide variety of widely used kits, the management of the kits produced has always been a very challenging task. At present, most manufacturers use the management method to record the relevant information of the kit in the paper document, and find the relevant documents to check if necessary. This manual recording and comparison management mode results in inefficient and high error rates for kit management, and the additional expenditure for "correction" generated each year amounts to millions or even tens of millions.

Problem solution

Technical solution

In order to solve the above problems in the prior art, the present invention provides a kit having a timing, a sample reminder, and a wireless management function.

[0007] The technical solution adopted by the present invention is: a kit with a metering, sample reminding and wireless management function, including a kit body, a lid, a liner, a timing device for implanting a kit, and a close range Wireless communication N FC tag. The counting device is composed of a control unit, a display module, a regulating button and a small battery. The display module and the regulating button are respectively electrically connected to the control unit, and the adjusting button adjusts the counting unit of the control unit according to the demand, and passes through the display module. Display relevant information. In the non-counting state, the display module can display the current time. The NFC tag of the short-range wireless communication is composed of a data storage module and a data transmission module. The output end of the data storage module is electrically connected to the data transmission module, and the data transmission module wirelessly transmits the signal to the intelligent management terminal used by the staff through the NFC chip.

[0008] The liner of the kit body is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicator light is further disposed between the two. The lining plate is spaced apart from the opening of the box body such that the lining plate forms an open receiving space with the box body; the reagent rotating disk is provided with a plurality of reagent holes, and A power mechanism is connected.

[0009] the reagent box body is further provided with an inductor, the sensor is disposed corresponding to a bottom of the pipette tip head collecting hole, and the sensor receives the hole by sensing the pipette tip head The bottom controls the rotation of the reagent rotating disk and the color of the arrow indicator light.

[0010] Further, the control unit is a single chip microcomputer.

[0011] Further, the display module is a liquid crystal digital display with a backlight.

[0012] Further, the small battery is a lithium ion battery. [0013] Further, the number of the control buttons is four, wherein three control the adjustment of hours, minutes, and seconds, and the other one controls the start/pause/end timing.

[0014] Further, the inductor is connected to the bottom of the pipette tip collecting hole by a spring.

[0015] Further, the reagent rotating disc is provided with 8 reagent holes; the power mechanism connected thereto is a stepping motor, and is connected to the inductor and controlled by the inductor.

[0016] Further, the arrow indicator is made of LED, connected to the sensor, and controlled by the sensor.

[0017] Further, the smart terminal used by the staff is set on the mobile phone and displays related information of the kit through the mobile phone interface.

Advantageous effects of the invention

Beneficial effect

[0018] The invention implants a timing module in the kit, which facilitates the experimenter to carry out the calibration step in the experiment process, enhances the continuity of the experiment, reduces the troubles that may be encountered in the experiment process, and finally improves the quality of the experimental result; In the non-timed state, the timer display can be used to display the current time to prevent the experimenter from missing other important matters. The reagent hole can be rotated by the reagent disk, which can help the experimenter to add the sample reminder to avoid the mistake of adding the wrong sample. The experiment failed; at the same time, the intelligent management terminal and the kit can realize the non-contact point-to-point data transmission within a distance of five centimeters, solving many problems of the traditional kit production management method, improving the efficiency and accuracy of management, for production Enterprises reduce management costs. Brief description of the drawing

DRAWINGS

1 is an overall configuration diagram of the present invention.

2 is a schematic view showing the structure of a reagent rotating disk, an arrow indicator lamp, and a pipette dp head collecting hole as shown in FIG. 1.

[0021] FIG. 3 is a schematic structural diagram of the short-range wireless communication NFC tag.

BEST MODE FOR CARRYING OUT THE INVENTION

[0022] The present invention will be further described below in conjunction with the accompanying drawings. [0023] As shown in FIG. 1, a kit having a timing, a sample reminder, and a wireless management function includes a kit body 10 and a lid 20, and the kit body is provided with a liner 30 and a timing device 40. And NFC tag 80 for short-range wireless communication. The timing device is composed of a control unit, a display module, a control button and a small battery. The display module and the control button are respectively electrically connected to the control unit, and the control button adjusts the timing of the control unit according to the demand, and the related information is displayed through the display module. display. In the non-timed state, the display module can display the current time.

[0024] As shown in FIG. 2, the lining plate 30 is provided with a pipette tip collecting hole 32 and a reagent rotating disk 36, and an arrow indicator 34 is further disposed between the two, and the reagent rotating disk 36 is disposed on the reagent rotating plate 36. Several reagent wells 38. The sensor 60 is disposed in the casing 10, and the inductor 60 is disposed corresponding to the bottom of the pipette tip collecting hole 32, and the inductor 60 controls the stepping motor 70 through the bottom of the inductive pipette tip collecting hole 32. The rotation of the reagent rotating disk 36 and the color of the arrow indicator light 34 are illuminated. With the above arrangement, the inductor 60 is connected to the bottom of the pipette tip head collecting hole 32 by a spring 50. When the loading operation is completed, the discarded tip is driven into the inductive pipette tip head collecting hole 32, and the sensor 60 passes through the inductive pipette tip to collect the bottom of the hole 32, and controls the breaking motor 70 to drive the reagent rotation. The color of the disk 36 is rotated and the arrow indicator light 34 is illuminated. The color of the arrow indicator light 34 is in one-to-one correspondence with the reagent holes on the reagent rotating disk 36. The eight colors of red, orange, yellow, green, cyan, blue, purple, and white correspond to 1-8 on the reagent rotating disk 36, respectively. Hole. Each time the sensor senses that the discarded tip is driven into the pipette tip collection hole, the control stepper motor 70 drives the reagent rotating disk 36 to rotate 45° to its next reagent hole, and controls the arrow indicator. 34 lights up the color of the corresponding reagent hole.

As shown in FIG. 3, the short-range wireless communication NFC tag mainly includes a data storage module 801, a data transmission module 802, and an NFC chip 803. The output of the data storage module is electrically connected to the data transmission module, and the data transmission module wirelessly transmits the signal to the intelligent management terminal (usually the mobile phone 90) used by the user through the NFC chip.

[0026] The kit with the function of counting and the reminding function of the sample is composed of a control unit, a metering device and a battery, and a sensor, a pipette tip collecting hole, and a reagent rotating disk. And the power mechanism can facilitate the experimenter to carry out the timing steps in the experiment process, improve the continuity of the experiment, reduce the troubles that may be encountered during the experiment, and finally improve the quality of the experimental results; in the non-timed state, the meter display It can be used to display the current time, to prevent the experimenter from missing other important matters; the peer can also function to instruct the experimenter to add reminders. [0027] The present invention is not limited to the above-described preferred embodiments, and any other form of product can be derived by anyone of the present invention, but without any change in its shape or structure, The same or similar technical solutions are all within the scope of the present invention.

Industrial applicability

[0028] The invention implants a timing module in the kit, which facilitates the experimenter to carry out the calibration step in the experiment process, enhances the continuity of the experiment, reduces the troubles that may be encountered in the experiment process, and finally improves the quality of the experimental result; In the non-counting state, the meter display can be used to display the current time to prevent the experimenter from missing other important matters. The reagent hole can be rotated by the reagent disk, which can help the experimenter to add the sample reminder. The experiment caused by the failure of the sample; at the same time, the intelligent management terminal and the kit can realize the non-contact point-to-point data transmission within a distance of five centimeters, solving many problems of the traditional kit production management method, and improving the management efficiency and accuracy. , reducing management costs for production companies.

Claims

[Claim 1] A test kit with timing, sample addition reminder and wireless management functions, characterized by including a test kit body, a lid, a liner, a measuring device implanted in the test kit and a short-range wireless communication NFC tag .

[Claim 2] A test kit with timing, sample addition reminder and wireless management functions according to claim 1, characterized in that the timing device is composed of a control unit, a display module, a control button and a small battery, and the display The module and the control button are electrically connected to the control unit respectively. The control button adjusts the timing of the control unit according to the needs, and displays the relevant information through the display module. In the non-counting state, the display module can display the current time.

[Claim 3] A reagent kit with timing, sample addition reminder and wireless management functions according to claim 1, characterized in that the lining plate is provided with a pipette tip collection hole and a reagent rotating disk, There is an arrow indicator light between the two. There is a certain distance between the lining plate and the opening of the box body, so that the lining plate and the box body form an open accommodation space; the reagent rotating disk is provided with a plurality of reagent holes, and is connected with the opening of the box body. A power mechanism is connected; a reagent tube arranged in the reagent hole;

A sensor is provided in the box body, the sensor is arranged corresponding to the bottom of the pipette tip collection hole, and the sensor controls the pipette tip by sensing the bottom of the pipette tip collection hole. The power mechanism drives the rotation of the reagent rotating disk and the color of the arrow indicator light.

[Claim 4] A test kit with timing, sample addition reminder and wireless management functions according to claim 1, the material of the arrow indicator light is LED, characterized in that, the arrow indicator light and the The sensor is connected and can light up eight colors of light: red, orange, yellow, green, cyan, blue, purple and white respectively as needed.

[Claim 5] A test kit with timing, sample addition reminder and wireless management functions according to claim 1, characterized in that the short-range wireless communication NFC tag mainly consists of a data storage module, a data transmission module and a Composed of NFC chip.

[Claim 6] A test kit with timing, sample addition reminder and wireless management functions according to claim 1, characterized in that the data storage module is electrically connected to the data transmission module, The data transmission module is electrically connected to the NFC chip and sends relevant data of the test kit to the intelligent management terminal through wireless signals.