WO2017214835A1

WO2017214835A1 - Reagent kit having cooling function and loading reminder function

Info

Publication number: WO2017214835A1
Application number: PCT/CN2016/085640
Authority: WO
Inventors: 彭鹏
Original assignee: 彭鹏
Priority date: 2016-06-14
Filing date: 2016-06-14
Publication date: 2017-12-21

Abstract

A reagent kit having a cooling function and loading reminder function. By providing a control unit, a temperature sensor and cooling device (40), and a storage battery to form a cooling system, and providing a sensor (60), a pipette tip collector (32), a reagent turntable (36), and a power mechanism, the present invention provides continuous and uniform refrigeration of the reagent kit when the reagent kit is not in a low-temperature environment, thus providing a favorable low-temperature environment for reagent loading, and preventing a reagent from degrading and becoming unsuitable for use due to loss of a low-temperature environment. At the same time, by driving rotation of reagent recesses (38) with the reagent turntable (36), the present invention achieves an effect of a loading reminder for lab personnel, thereby preventing an experiment failure due to a loading error.

Description

Kit with cold keeping function and sample reminding function

[0001] The present invention relates to the field of biotechnology, and in particular to a reagent cartridge having a cooling function and a sample reminding function.

Background technique

[0002] In the field of biotechnology, in addition to technical breakthroughs, it is more important to improve the efficiency of research and experimentation. Among them, the emergence of kits plays an important role. The kit standardizes the steps of common biological experiments, providing the experimenter with the reagents to be prepared and the optimized loading method, which greatly increases the speed of the experimental development and the degree of standardization of the results.

technical problem

[0003] Due to the biological characteristics, many reagents in the use of hydrazine to ensure the effectiveness of the reagents and the accuracy of the experiment, need to be completed in a low temperature (2-8 ° C) environment, and currently, such operations are usually used " The ice box is "completed, the ice cubes are added to the appropriate size of the foam box, and the reagent tube is inserted into the ice to ensure that the reagents are at the desired low temperature. However, because many biological experiments require aseptic processing, and ice cubes are easily stained with bacteria in the air, which is a potential source of bacterial contamination in experiments, low-temperature loading operations using "ice boxes" are used in these experiments.吋 The requirements for the operation of the experimenter are higher, the burden on the experimenter is increased, and the experimental success rate cannot be guaranteed.

[0004] In addition, as the complexity of the experiment is increasing, the number of reagents in the existing kits is also increasing, and the step of adding samples is becoming more and more complicated. Take the commonly used real-time PCR kit as an example. The general kit contains Taq enzyme, buffer, chromogenic dye and sterile ultrapure water. In addition, the reagents required by the experimenter include at least the upstream primer. Downstream primers and template, a total of 7 reagents together to prepare a fluorescent quantitative PCR reaction system. In many cases, the experimenter will have the phenomenon of missing, adding, adding, etc. during the process of adding samples. The reason is that there are many kinds of reagents, and the process of loading cannot be well identified and marked.吋 Handling other urgent matters and causing ongoing experiments to be interrupted, errors are inevitable.

Problem solution Technical solution

In order to solve the above problems in the prior art, the present invention provides a kit having a cold keeping function and a loading and lifting function.

[0006] The technical solution adopted by the present invention is: a kit having a cold keeping function and a sample reminding function, comprising a kit body and a lid, wherein the kit body is provided with a plurality of reagent holes, a temperature sensor and a plurality of In the cooling device, a control unit and a small storage battery are disposed in the reagent body, and the control unit, the cooling device, the temperature sensor and the storage battery are electrically connected.

[0007] The inner layer of the kit body is further provided with a lining plate, and the lining plate is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicator light is further disposed between the two. The lining plate is spaced apart from the mouth of the box body such that the lining plate forms a clogging space with the box body; the reagent rotating disk is provided with a plurality of reagent holes, And connected to a power mechanism.

[0008] the reagent box body is further provided with an inductor, the sensor is disposed corresponding to a bottom of the pipette tip head collecting hole, and the sensor receives the hole by sensing the pipette tip head The bottom controls the rotation of the reagent rotating disk and the color of the arrow indicator light.

Further, the kit body has a two-layer structure, and the cooling device is disposed at an equal interval between the inner layer and the outer layer of the kit body.

[0010] Further, the kit body is provided with four cooling devices at equal intervals on each side.

[0011] Further, the inductor is connected to the bottom of the pipette tip collecting hole by a spring.

[0012] Further, the reagent rotating disk is provided with 8 reagent holes; the power mechanism connected thereto is a stepping motor, and is connected to the inductor and controlled by the inductor.

[0013] Further, the arrow indicator is made of LED, connected to the inductor, and controlled by the inductor.

Beneficial effect

[0014] The present invention provides a two-layer structure of the kit body, and a cooling device is disposed between the inner layer and the outer layer of the kit body, and the cooling system is composed of a control unit, a temperature sensor cooling device and a battery, and the kit can be used in the kit. Loss of low temperature environment, continuous and even cooling of the kit, providing a good low temperature environment for reagent loading, and avoiding the deterioration of reagents due to loss of low temperature environment. Peer, passing reagent The disk drives the reagent hole to rotate, which can serve as an additive reminder for the experimenter, and avoid the experiment failure caused by the wrong sample.

Brief description of the drawing

DRAWINGS

1 is an overall configuration diagram of the present invention.

2 is a schematic view showing the structure of a reagent rotating disk, an arrow indicator lamp, and a pipette tip head collecting hole as shown in FIG. 1. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

BEST MODE FOR CARRYING OUT THE INVENTION

[0017] The present invention will be further described below in conjunction with the accompanying drawings.

[0018] As shown in FIG. 1 , a kit having a cold keeping function and a sample reminding function includes a kit body 10 and a lid 20 , and the kit body is provided with a liner 30 and a temperature sensor (in the figure) Not shown) and a number of cooling devices 40, a control unit (not shown) and a small battery (not shown) are provided in the kit body. The control unit, the cooling device, the temperature sensor and the battery are electrically connected.

[0019] As shown in FIG. 2, the lining plate 30 is provided with a pipette tip collecting hole 32 and a reagent rotating disk 36, and an arrow indicator light 34 is disposed between the two, and the reagent rotating disk 36 is disposed on the reagent rotating plate 36. Several reagent wells 38. The sensor 60 is disposed in the casing 10, and the inductor 60 is disposed corresponding to the bottom of the pipette tip collecting hole 32, and the inductor 60 controls the stepping motor 70 through the bottom of the inductive pipette tip collecting hole 32. The rotation of the reagent rotating disk 36 and the color of the arrow indicator light 34 are illuminated. With the above arrangement, the inductor 60 is connected to the bottom of the pipette tip head collecting hole 32 by a spring 50. When the loading operation is completed, the discarded tip is driven into the inductive pipette tip head collecting hole 32, and the sensor 60 controls the stepping motor 70 to drive the reagent rotation by inducing the pipette tip to collect the bottom of the hole 32. The color of the disk 36 is rotated and the arrow indicator light 34 is illuminated. The color of the arrow indicator light 34 is in one-to-one correspondence with the reagent holes on the reagent rotating disk 36. The eight colors of red, orange, yellow, green, cyan, blue, purple, and white correspond to 1-8 on the reagent rotating disk 36, respectively. Hole. Each time the sensor senses that the discarded tip is driven into the pipette tip collection hole, the control stepper motor 70 drives the reagent rotating disk 36 to rotate 45° to its next reagent hole, and controls the arrow indicator. 34 lights up the color of the corresponding reagent hole.

[0020] The kit with the cold keeping function and the sample reminding function comprises a cooling unit composed of a control unit, a temperature sensor cooling device and a battery, and a sensor, a pipette tip collecting hole, and a reagent. The rotating disc and the power mechanism can continuously and uniformly cool the kit after the kit loses the low temperature environment, providing a good low temperature environment for reagent loading, and avoiding the reagent being deteriorated due to loss of low temperature environment; The disk drives the reagent hole to rotate, which can serve as an additive reminder for the experimenter, and avoid the experiment failure caused by the wrong sample.

[0021] The present invention is not limited to the above-described preferred embodiments, and any other form of product can be derived by anyone of the present invention, but without any change in its shape or structure, The same or similar technical solutions are all within the scope of the present invention.

Industrial applicability

[0022] The present invention provides a double-layer structure, and a cooling device is disposed between the inner layer and the outer layer of the reagent cartridge body, and a cooling system is formed by the control unit, the temperature sensor cooling device, and the storage battery, and the reagent cartridge can be Loss of low temperature environment, continuous and even cooling of the kit, providing a good low temperature environment for reagent loading, and avoiding the deterioration of reagents due to loss of low temperature environment. At the same time, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, and avoid the experiment failure caused by the wrong sample.

Claims

claims

[Claim 1] A reagent kit with a cold preservation function and a sample addition reminder function, which is characterized in that it includes a reagent kit body and a lid. The reagent kit body is provided with a liner, a temperature sensor and several cooling devices. The reagent kit The main body is provided with a control unit and a small battery, and the control unit, cooling device, temperature sensor and battery are electrically connected.

[Claim 2] A reagent kit with a cold preservation function and a sample addition reminder function according to claim 1, characterized in that the lining plate is provided with a pipette tip collection hole and a reagent rotating disk, both of which are There is also an arrow indicator light between them. There is a certain distance between the lining plate and the opening of the box body, so that the lining plate and the box body form an open accommodation space; a plurality of reagent holes are provided on the reagent rotating disk, And connected to a power mechanism; a reagent tube arranged in the reagent hole;

A sensor is provided in the box body, the sensor is arranged corresponding to the bottom of the pipette tip collection hole, and the sensor controls the pipette tip by sensing the bottom of the pipette tip collection hole. The power mechanism drives the rotation of the reagent rotating disk and the color of the arrow indicator light.

[Claim 3] A test kit with a cold preservation function and a sample addition reminder function according to claim 1, the material of the arrow indicator light is LED, characterized in that, the arrow indicator light and the sensor connected, and can light up eight colors of red, orange, yellow, green, cyan, blue, purple and white respectively as needed.

[Claim 4] A reagent kit with a cold preservation function and a sample addition reminder function according to claim 1, characterized in that the reagent kit body has a double-layer structure, and the cooling devices are arranged at equal intervals on the reagent kit body. between the inner and outer layers.

[Claim 5] A reagent kit with a cold preservation function and a sample addition reminder function according to claim 1, characterized in that four cooling devices are provided at equal intervals on each side of the reagent kit body.